WO2024119480A1 - Focusing control method and related apparatus - Google Patents

Abstract

Disclosed in the present application are a focusing control method and a related apparatus. The focusing control method is used for a focusing platform system, which comprises a focusing device, a micro-motion platform and a macro-motion platform, the focusing device being mounted on the micro-motion platform, and the macro-motion platform carrying a test sample, or, the micro-motion platform being mounted on the macro-motion platform, and the micro-motion platform carrying the test sample. The focusing control method comprises: starting the focusing device; obtaining a first target displacement of movement of the macro-motion platform, and driving the macro-motion platform to move to a first target position; and obtaining a second target displacement of movement of the micro-motion platform, and driving the micro-motion platform to move to a second target position. In the present application, by means of large-stroke rapid adjustment of the macro-motion platform for a focusing position, combined with high-precision adjustment of the micro-motion platform, a highly stable, rapid, large-stroke, high-precision optical focusing positioning movement is implemented.

Description

Focus control method and related equipment Technical Field

The present application relates to the field of focus control technology, and in particular to a focus control method, a focus control device, an electronic device, a computer-readable medium, a nucleic acid detection system, and a focusing method for a nucleic acid detection system.

Background technique

Initially, gene sequencing technology was performed manually. However, due to the low efficiency of manual operation and the proneness to human errors, sequencing using gene sequencers has now become the mainstream sequencing technology.

The sequencing process of current gene sequencers consists of a series of mechanical, electronic communication, biological, chemical, and optical operations, which are performed by the corresponding components in the gene sequencer, replacing simple manual operations. However, gene sequencing also faces the following problems: On the one hand, due to the very high precision requirements of the corresponding platform for gene sequencing, which is at the sub-micron level, any deviation in the operation of a component will lead to an unsatisfactory sequencing structure; on the other hand, the specific steps involved in the entire sequencing process are very cumbersome, requiring the various components of the sequencer to work together. Among them, optical focusing plays a pivotal role in the entire sequencing system.

The precision motion platforms in the current mainstream high-end sequencer optical mirror detection equipment and high-end manufacturing equipment are divided into single-stage drive and two-stage drive in terms of drive mode, and their drive motors are divided into linear motors and rotary motors. The precision motion platforms driven by single-stage drive and rotary motor plus lead screw generally have a large stroke, which can easily reach several thousand millimeters, but their accuracy can only reach a few microns, which can no longer meet the high-precision requirements in many fields. The motion platforms driven by shape memory alloys, giant magnetostrictors, piezoelectric ceramics, etc. can achieve sub-nanometer accuracy, but the stroke is very small, often only a few hundred microns. These motion actuators cannot meet the requirements of large stroke and high precision at the same time.

Application Contents

The present application proposes a focus control method, a focus control device, an electronic device, a computer-readable medium, a nucleic acid detection system, and a focusing method of a nucleic acid detection system to achieve high-stability, fast, large-stroke, and high-precision optical focus positioning movement.

In order to achieve the above objectives, this application provides the following technical solutions:

In a first aspect, the present application provides a focus control method for a focus platform system, wherein the focus platform system includes a focus device, a micro-motion platform, and a macro-motion platform; the focus device is mounted on the micro-motion platform, and the macro-motion platform carries a sample to be detected; or, the micro-motion platform is mounted on the macro-motion platform, and the micro-motion platform carries a sample to be detected; the focus control method includes:

Starting the focusing device, adjusting the macro-motion platform to drive the sample to be detected to move relative to the focusing device, so that the focusing device receives the optical signal of the sample to be detected;

Determine a target focus position of the macro-motion platform along the optical axis direction of the focusing device based on the optical signal and drive the macro-motion platform to move a first target displacement amount along the optical axis direction to a first target position;

Obtaining an error value between the first target position and the target focus position;

A second target displacement is acquired based on the error value, and the micro-motion platform is driven according to the second target displacement to adjust the relative position between the focusing device and the sample to be detected.

In some possible solutions of the present application, the macro-motion platform is a linear motor control platform;

The micro-motion platform is a piezoelectric ceramic control platform.

In some possible solutions of the present application, the focus control method further includes: detecting and feeding back in real time during the movement of the micro-motion platform the relative position between the focus device and the sample to be detected and adjusting a driving signal of the micro-motion platform.

In some possible solutions of the present application, before driving the macro-motion platform to move to the first target displacement and before driving the micro-motion platform to adjust the relative position between the focusing device and the sample to be detected, the method further includes: determining whether the focusing device works normally and whether the focusing signal of the focusing device is within the working range, if so, executing the subsequent steps, if not, reporting an error.

In some possible solutions of the present application, the normal operation of the focusing device specifically means that the focusing device transmits a normal signal;

The focusing signal of the focusing device includes a sum signal and a difference and division signal.

In some possible solutions of the present application, after driving the macro-motion platform to move to the first target position, the method further includes:

Determine whether the focusing is successful;

If the focusing fails, check whether the focusing instruction to stop the focusing device is valid. If it is valid, end the process. If it is invalid, return to the step corresponding to the macro-motion platform to determine whether the focusing device works normally and whether the focusing signal of the focusing device is within the working range.

If the focusing is successful, it is determined whether the focusing of the focusing device has entered a stable state. If the focusing has not entered a stable state, the process returns to the step of checking whether the stop focusing instruction is valid. If the focusing has entered a stable state, the macro motion platform is locked.

In some possible schemes of the present application, after driving the micro-motion platform to adjust the relative position between the focusing device and the sample to be detected according to the second target displacement, it also includes: checking whether the stop focusing instruction is valid, if so, ending, if not, returning to the step corresponding to the micro-motion platform to determine whether the focusing device is working normally and whether the focusing signal of the focusing device is within the working range.

In some possible solutions of the present application, before starting the focusing device, the method further includes: initializing the micro-motion platform and driving the macro-motion platform to enter an initial focusing position.

In some possible solutions of the present application, the sample to be detected is a tissue sample.

In some possible schemes of the present application, the sample to be detected is a nucleic acid tissue library.

In a second aspect, the present application provides a focus control device, which is applied to a focus platform system, wherein the focus platform system includes a focus device, a micro-motion platform, and a macro-motion platform; the focus device is mounted on the micro-motion platform, and the macro-motion platform carries a sample to be detected; or, the micro-motion platform is mounted on the macro-motion platform, and the micro-motion platform carries a sample to be detected;

The focus control device further comprises: a focus module connected to the focus device, a micro-motion platform control module connected to the micro-motion platform, and a macro-motion platform control module connected to the macro-motion platform;

The macro-motion platform drives the sample to be detected to move relative to the focusing device, so that the focusing device receives the optical signal of the sample to be detected, and the focusing module obtains the initial displacement of the macro-motion platform according to the optical signal, and sends the initial displacement to the macro-motion platform control module;

The macro-motion platform control module controls the macro-motion platform to move to a position where the focusing device receives the optical signal of the sample to be detected;

The focusing module further obtains a first target displacement amount of the macro-motion platform required to move to a target focusing position along the optical axis direction of the focusing device, and sends the first target displacement amount to the macro-motion platform control module;

The macro-motion platform control module controls the macro-motion platform to move to a first target position based on the first target displacement;

The focusing module obtains an error value between the first target position and the target focusing position, obtains a second target displacement amount required for the micro-motion platform to move based on the error value, and sends the second target displacement amount to the micro-motion platform control module;

The micro-motion platform control module controls the movement of the micro-motion platform to adjust the relative position between the focusing device and the sample to be detected.

In some possible solutions of the present application, the focusing module obtains the relative position between the focusing device and the sample to be detected in real time during the movement of the micro-motion platform, obtains the real-time displacement of the second target based on the real-time relative position, and sends it to the micro-motion platform control module;

The micro-motion platform control module controls the micro-motion platform to move according to the real-time second target displacement.

In some possible solutions of the present application, the focus control device further includes a signal determination module;

Before the focusing module obtains the first target displacement amount required for the macro-motion platform to move, the signal judgment module determines whether the focusing device works normally and whether the focusing signal of the focusing device is within the working range. If so, the focusing device is triggered to execute the acquisition of the first target displacement amount of the macro-motion platform. If not, an error is reported.

Before the focusing module obtains the second target displacement amount required for the micro-motion platform to move, the signal judgment module determines whether the focusing device is working normally and whether the focusing signal of the focusing device is within the working range. If so, the focusing device is triggered to execute the acquisition of the second target displacement amount of the micro-motion platform. If not, an error is reported.

In some possible solutions of the present application, after driving the macro-motion platform to move to the first target position, the signal determination module further determines whether the focusing device is successfully focused.

When focusing fails, determining whether the stop focusing instruction of the focusing device is valid; if not, determining whether the focusing device works normally and whether the focusing signal of the focusing device is within the working range; if valid, ending;

When the focusing is successful, determine whether the focus of the focusing device has entered a stable state. If the focus has entered a stable state, trigger the macro-motion platform control module to lock the macro-motion platform. If the focus has not entered a stable state, determine whether the focus stop instruction of the focusing device is valid. If not, determine whether the focusing device is working normally and whether the focus signal of the focusing device is within the working range. If valid, end.

In some possible solutions of the present application, after the micro-motion platform moves to the second target position, the signal judgment module further judges whether the stop command of the focusing device is valid. If so, the operation is terminated; if not, the control module judges whether the focusing device works normally and whether the focusing signal of the focusing device is within the working range. If so, an error is reported; if not, the micro-motion platform control module is triggered to control the micro-motion platform to move to the second target position.

In some possible solutions of the present application, the focus control device further includes an initialization control module;

The initialization control module controls the micro-motion platform to move to an initial position, and controls the macro-motion platform to move to an initial focusing position.

In a third aspect, the present application provides an electronic device, comprising a processor, a memory and a communication bus, wherein the processor and the memory are connected to each other via the communication bus, and the memory stores at least one or more programs;

The processor calls the program stored in the memory and executes the focus control method as described in any one of the above items.

In a fourth aspect, the present application provides a computer-readable medium having a computer program stored thereon, wherein the computer program, when executed by a processor, implements a focus control method as described in any one of the above.

In a fifth aspect, the present application provides a detection device, including an optical system and a focusing platform system as described in any one of the above items corresponding to the optical system.

In some possible solutions of the present application, the detection device is used for gene detection, and the focusing platform system supports the gene detection sample and focuses the position of the gene detection sample relative to the optical system.

In a sixth aspect, the present application provides a focusing method for a nucleic acid detection system, comprising:

Adjusting the macro-motion platform to drive the sequencing chip to move relative to the objective lens, so that the objective lens receives the optical signal of the sequencing chip;

Determine a target focus position of the macro-motion platform along the optical axis direction of the objective lens based on the optical signal and drive the macro-motion platform to move a first target displacement amount along the optical axis direction to a first target position;

Acquire an error value between the first target position and the target focus position; and

Acquire a second target displacement based on the error value and drive the micro-motion platform to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement;

The objective lens is mounted on the micro-motion platform, and the macro-motion platform is used to carry the sequencing chip; or the micro-motion platform is mounted on the macro-motion platform, and the micro-motion platform is used for the sequencing chip.

In some possible solutions of the present application, the macro-motion platform is a linear motor control platform;

The micro-motion platform is a piezoelectric ceramic control platform.

In some possible solutions of the present application, the focusing method further includes: detecting and feeding back the relative position between the objective lens and the sequencing chip in real time during the movement of the micro-motion platform and adjusting the driving signal of the micro-motion platform.

In some possible solutions of the present application, before driving the macro-motion platform to move to the first target displacement and before driving the micro-motion platform to adjust the relative position between the objective lens and the sequencing chip, it also includes: judging whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range, if so, executing subsequent steps, if not, reporting an error.

In some possible solutions of the present application, the normal operation of the objective lens specifically means that the objective lens transmits a normal signal;

The focus signal of the objective lens includes a sum signal and a difference and division signal.

In some possible solutions of the present application, after driving the macro-motion platform to move to the first target position, the method further includes:

Determine whether the focusing is successful;

If the focusing fails, check whether the focusing instruction to stop the objective lens is valid, if it is valid, end, if not, return to the step corresponding to the macro-motion platform to determine whether the objective lens works normally and whether the focusing signal of the objective lens is within the working range;

If the focusing is successful, it is determined whether the focusing of the objective lens enters a stable state. If the focusing does not enter a stable state, the process returns to the step of checking whether the stop focusing instruction is valid. If the focusing enters a stable state, the macro motion platform is locked.

In some possible schemes of the present application, after driving the micro-motion platform to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement, the method further includes: checking whether the stop focus instruction is valid, and if so, ending; if not, returning to the step corresponding to the micro-motion platform to determine whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range.

In some possible solutions of the present application, before starting the focusing device, the method further includes: initializing the micro-motion platform and driving the macro-motion platform to enter an initial focusing position.

In a seventh aspect, the present application provides a nucleic acid detection system, comprising a sequencing chip and an optical system, wherein the optical system comprises an objective lens for receiving an optical signal of the sequencing chip, and the nucleic acid detection system further comprises a macro-motion platform and a micro-motion platform;

The objective lens is mounted on the micro-motion platform, and the macro-motion platform is used to carry the sequencing chip; or the micro-motion platform is mounted on the macro-motion platform, and the micro-motion platform is used to carry the sequencing chip.

The nucleic acid detection system further includes a focus control mechanism associated with the macro-motion platform and the micro-motion platform, and the focus control mechanism is used to:

Adjusting the macro-motion platform to drive the sequencing chip to move relative to the objective lens, so that the objective lens receives the optical signal of the sequencing chip;

Determine a target focus position of the macro-motion platform along the optical axis direction of the objective lens based on the optical signal and drive the macro-motion platform to move a first target displacement amount along the optical axis direction to a first target position;

Acquire an error value between the first target position and the target focus position; and

A second target displacement is obtained based on the error value, and the micro-motion platform is driven according to the second target displacement to adjust the relative position between the objective lens and the sequencing chip.

In some possible solutions of the present application, during the movement of the micro-motion platform, the focus control mechanism detects and feeds back the relative position between the objective lens and the sequencing chip in real time, and drives the micro-motion platform to move according to the relative position.

In some possible solutions of the present application, the nucleic acid detection system further includes a signal determination device;

Before obtaining the first target displacement amount required for the macro-motion platform to move, the signal judgment device judges whether the objective lens works normally and whether the focus signal of the objective lens is within the working range. If so, the objective lens is triggered to execute the acquisition of the first target displacement amount of the macro-motion platform. If not, an error is reported.

Before obtaining the second target displacement amount required for the micro-motion platform to move, the signal judgment device judges whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If so, the objective lens is triggered to execute the acquisition of the second target displacement amount of the micro-motion platform movement. If not, an error is reported.

In some possible solutions of the present application, after the macro-motion platform moves to the first target position, the signal determination device further determines whether the objective lens is focused successfully.

When focusing fails, judging whether the stop focusing instruction of the objective lens is valid, if not valid, judging whether the objective lens works normally and whether the focus signal of the objective lens is within the working range, if valid, ending;

When the focusing is successful, determine whether the focus of the objective lens enters a stable state. If the focus enters a stable state, trigger the focus control mechanism to lock the macro-motion platform. If the focus does not enter a stable state, determine whether the stop focus instruction of the objective lens is valid. If not, determine whether the objective lens works normally and whether the focus signal of the objective lens is within the working range. If valid, end.

In some possible schemes of the present application, after the micro-motion platform moves to the second target position, the signal judgment device further judges whether the stop command of the objective lens is valid. If it is valid, it ends; if it is invalid, it judges whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If so, an error is reported; if not, the focus control mechanism is triggered to control the micro-motion platform to move to the second target position.

In some possible solutions of the present application, the nucleic acid detection system further includes an initialization control device;

The initialization control device controls the micro-motion platform to move to an initial position, and controls the macro-motion platform to move to an initial focusing position.

It can be seen from the above technical solution that the large-stroke rapid adjustment of the focus position is achieved through the macro-motion platform, combined with the high-precision adjustment of the micro-motion platform, to achieve high-stability, fast, large-stroke and high-precision optical focus positioning movement.

BRIEF DESCRIPTION OF THE DRAWINGS

In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following briefly introduces the drawings required for use in the embodiments or the prior art description.

BRIEF DESCRIPTION OF THE DRAWINGS

In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only some examples or embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without creative work, and the present application can also be applied to other similar scenarios based on the provided drawings. Unless it is obvious from the language environment or otherwise explained, the same reference numerals in the figures represent the same structure or operation.

FIG1 is a flow chart of an embodiment of a focus control method provided by the present application;

FIG2 is a flow chart of another embodiment of a focus control method provided by the present application;

FIG3 is a schematic diagram of the structure of an electronic device provided by the present application;

FIG4 is a schematic diagram of a three-dimensional structure of a focusing platform system provided in the present application;

FIG5 is a schematic diagram of the main structure of the focusing platform system provided by the present application;

FIG. 6 is a block diagram corresponding to the focus control device provided in the present application.

in:

Focusing platform system 1000, macro-motion platform 100, micro-motion platform 200, focusing device 300, first moving stage 101, fixed seat 102, first moving seat 103, second moving seat 104, rotating shaft 105, sample platform 106, bracket 400, and sample to be detected 500.

Detailed ways

The present application will be further described in detail below in conjunction with the accompanying drawings and embodiments. It will be understood that the specific embodiments described herein are only used to explain the related application, rather than to limit the application. The described embodiments are only a part of the embodiments of the present application, rather than all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the field without making creative work are within the scope of protection of the present application.

In the first aspect, please refer to Figures 1-2, the focus control method in the embodiment of the present application is used for the focus platform system 1000 to achieve high stability, fast, large stroke and high precision optical focus positioning movement.

The focusing platform system 1000 includes a focusing device 300, a micro-motion platform 200, and a macro-motion platform 100. As shown in FIG3 , the focusing device 300 is mounted on the micro-motion platform 200, and the macro-motion platform 100 is used to carry the sample to be detected 500. It should be noted that it can also be set that the micro-motion platform 200 is mounted on the macro-motion platform 100, and the micro-motion platform 200 is used to carry the sample to be detected 500. It can be understood that the focusing device 300 and the sample to be detected 500 are arranged face to face and separated by a certain distance to facilitate focus adjustment.

As shown in FIG. 1 , the focus control method includes step S1 , step S2 and step S3 .

Step S1: Start the focusing device 300 and adjust the movement of the macro-motion platform 100 so that the focusing device 300 receives the optical signal of the sample 500 to be detected.

Specifically, the macro-motion platform 100 is adjusted to move, thereby driving the sample 500 to be detected to move relative to the focusing device 300 , so that the focusing device 300 can receive the optical signal of the sample 500 to be detected.

The focusing device 300 includes an objective lens, and the focusing setting is achieved by adjusting the distance between the objective lens and the sample 500 to be detected.

Here, adjusting the macro-motion platform 100 to drive the sample to be detected 500 to move means adjusting the macro-motion platform 100 to drive the sample to be detected 500 to move in the direction of the optical axis of the focusing device 300 .

Step S2: determining a target focusing position of the macro-motion platform 100 along the optical axis direction of the focusing device 300 and driving the macro-motion platform 100 to move a first target displacement to the first target position.

Specifically, the target focusing position of the macro-motion platform 100 along the optical axis direction of the focusing device 300 is determined based on the optical signal, and the macro-motion platform 100 moves along the optical axis direction.

Specifically, distance detection can be performed through a photoelectric sensor. The photoelectric sensor is a two-pixel photodiode. The two pixels of the two-pixel photodiode are symmetrically distributed with the optical axis of the objective lens as the center. The two-pixel photodiode outputs two light signals according to the photosensitivity of the two pixels. The first target displacement is calculated by dividing the difference of the two light signals by 0.

It should be noted that the first target position refers to the position reached by the macro motion platform 100 after moving according to the first target displacement amount.

Specifically, the macro-motion platform is a platform driven by a driver that realizes large-stroke, high-speed motion, such as a linear motor controlled platform.

Step S3: acquiring a second target displacement and driving the fine motion platform 200 to move according to the second target displacement.

The error value between the first target position and the target focusing position is obtained, and the second target displacement is obtained based on the error value. The micro-motion platform 200 is driven according to the second target displacement to adjust the relative position between the focusing device 300 and the sample to be detected 500.

The focusing device 300 can always be in a state where the automatic focusing program is turned on, so as to facilitate the adjustment of the micro-motion platform 200 and the macro-motion platform 100, avoid the reciprocating opening and closing of the focusing device 300, and shorten the focusing time.

The fine motion platform 200 is a platform driven by a driver for achieving precise motion, for example, a piezoelectric ceramic control platform or a magnetostrictive material control platform.

The present application realizes large-stroke rapid adjustment of the focus position through the macro-motion platform 100, and combines the high-precision adjustment of the micro-motion platform 200 to achieve high-stability, fast, large-stroke and high-precision optical focus positioning movement.

In some examples of the present application, step S3 further includes: detecting and feeding back the relative position between the focusing device 300 and the sample to be detected 500 in real time during the movement of the fine motion platform 200 and adjusting the driving signal of the fine motion platform 200 .

The present application realizes real-time adjustment of the movement of the micro-motion platform 200 according to the real-time relative position between the focusing device 300 and the sample to be detected 500 during the movement of the micro-motion platform 200, thereby realizing precise adjustment of the micro-motion platform 200.

In some examples of the present application, please refer to Figure 2, step S4 is also included between step S1 and step S2: determine whether the focusing device 300 is working normally and whether the focusing signal of the focusing device 300 is within the working range, if so, go to step S2, if not, report an error.

It should be noted that the normal operation of the focusing device 300 here means that the focusing device 300 transmits signals normally, that is, the received signals and the sent signals are normal.

Whether the focus signal of the focus device 300 is within the working range refers to whether the image captured by the focus device 300 can be switched between clear and blurred.

In some examples of the present application, the present application also discloses that the focus signal of the focus device 300 includes a sum signal and a difference and division signal.

It should be noted that the two-pixel photodiode outputs two light signals according to the photosensitivity of the two pixels, the sum of the two light signals is the sum signal, and the ratio of the difference between the two light signals and the sum of the two light signals is the difference sum division signal.

Furthermore, the present application discloses that the following steps are included between step S2 and step S3.

Step S5: Determine whether the focusing is successful, if not, go to step S6, if yes, go to step S7.

Step S6: Check whether the stop focus instruction is valid, if not, go to step S4, if yes, end.

The end here means stopping the focusing work of the entire focusing platform system 1000, so that manual maintenance can be performed, etc., avoiding invalid focusing and further saving time.

Step S7: Determine whether the focus has entered a stable state. If not, go to step S6; if so, go to step S8.

Step S8: Locking the position of the macro-motion platform 100 .

The present application locks the movement of the macro-motion platform 100 to avoid the macro-motion platform 100 being displaced after the adjustment of the macro-motion platform 100 to affect the accuracy of the adjustment.

It should be noted that locking the position of the macro motion platform 100 can be achieved by locking the macro motion driver, or by setting other locking structures that limit the movement of the macro motion platform 100 in the first direction.

In some examples of the present application, step S9 is also included between step S8 and step S3: determining whether the focusing device 300 works normally and whether the focusing signal of the focusing device 300 is within the working range, if so, go to step S3, if not, report an error.

Furthermore, after step S9, the process further includes step S10: checking whether the stop focus instruction is valid, if not, going to step S9, and ending the process if yes.

In order to improve the focusing accuracy, the present application discloses that before step S1 , the process further includes step S11 : initializing the micro-motion platform 200 , and driving the macro-motion platform 100 to enter an initial focusing position.

Specifically, initialization of the micro-motion platform 200 refers to the micro-motion platform 200 moving to an initial position, and entry of the macro-motion platform 100 into an initial focusing position refers to the macro-motion platform 100 moving to an initial position.

In some examples of the present application, the sample 500 to be detected is a tissue sample. It should be noted that it can also be a DNA chip, etc.

More specifically, the present application discloses that the sample to be detected 500 is a nucleic acid tissue library. It is understandable that the sample to be detected 500 disclosed in the present application as a nucleic acid tissue library is only a specific embodiment of the present application. In practical applications, the sample to be detected 500 can also be selected as other biochemical detection samples as needed.

On the second aspect, the present application provides an implementation of the methods shown in some of the above figures. The present application provides an embodiment of a focus control device. The device embodiment corresponds to the method embodiment shown in Figure 2, and the device can be specifically applied to various electronic devices.

Taking the application of the focus control device to the focus platform system 1000 as an example, specifically, the focus platform system 1000 includes a focus device 300, a micro-motion platform 200 and a macro-motion platform 100, the focus device 300 is installed on the micro-motion platform 200, and the macro-motion platform 100 is used to carry the sample to be detected 500. It should be noted that the focus device 300 is installed on the micro-motion platform 200, and the macro-motion platform 100 is used to carry the sample to be detected 500 is only a specific implementation of the present application. In actual applications, the micro-motion platform 200 can also be installed on the macro-motion platform 100, and the micro-motion platform 200 is used to carry the sample to be detected 500.

The focus control device includes a focus module, a micro-motion platform control module and a macro-motion platform control module. The focus module obtains the initial displacement of the sample 500 to be detected driven by the macro-motion platform 100 relative to the focus device 300, so that the focus device 300 receives the initial displacement of the optical signal of the sample 500 to be detected, and sends the initial displacement to the macro-motion platform control module.

The focusing module is also used to obtain a first target displacement amount required for the macro-motion platform 100 to move to a target focusing position along the optical axis direction of the focusing device 300, and send the first target displacement amount to the macro-motion platform control module.

The focusing module specifically calculates the first target displacement through the difference, sum and difference and division signals transmitted by the two-pixel photodiode.

The macro-motion platform control module is used to control the macro-motion platform 100 to move to the position where the focusing device 300 receives the optical signal of the sample 500 to be detected, and controls the macro-motion platform 100 to move to the first target position according to the first target displacement signal sent by the focusing module.

Specifically, the macro-motion platform control module controls the macro-motion driver to drive the macro-motion platform 100 to move to the first target position according to the first target displacement signal transmitted by the focus module.

The focusing module is also used to obtain an error value between the first target position and the target focusing position, and obtain a second target displacement amount required to be moved by the micro-motion platform 200 based on the error value, and send the second target displacement amount to the micro-motion platform control module.

It should be noted that the focusing module calculates the second target displacement through the difference, sum and difference and division signals transmitted by the two-pixel photodiode.

The micro-motion platform control module is used to control the micro-motion platform 200 to move to the second target position, so as to adjust the relative position between the focusing device 300 and the sample 500 to be detected.

FIG6 is a block diagram corresponding to the focus control device, wherein G1 is a macro-motion platform, C1 and P1 are the compensator and controller of the linear motor in the macro-motion platform, G2 is a micro-motion platform, C2 and P2 are the compensator and controller of the piezoelectric ceramic, RC is the input position command (i.e., target position information), P is the absolute position of the end of the entire macro-micro platform, and D is an external interference signal (such as electromagnetic interference). Two feedback signals are used to feedback the total position output of the entire focus platform system, one feedback signal is feedback signal S1, and the other feedback signal is feedback signal S2. The error signal between the feedback signal S1 and the input target position signal will be fed back to the compensator C1 of the macro-motion platform and the compensator C2 of the micro-motion platform at the same time, without the need to observe each actuator separately. When the error signal is greater than the preset error value (the maximum stroke of the micro-motion platform), the macro-motion platform moves further to control the error value within the stroke of the micro-motion platform; when the error signal is less than or equal to the stroke of the micro-motion platform, the micro-motion platform is started, and another feedback signal S2 is fed back to the control end of the micro-motion platform in real time, and the drive signal of the micro-motion platform is adjusted in real time based on the feedback signal S2 (negative feedback).

In order to improve the accuracy of the movement of the micro-motion platform 200, the present application discloses that the focusing module is also used to obtain the relative position between the focusing device 300 and the sample to be detected 500 in real time during the movement of the micro-motion platform 200, obtain the real-time second target displacement based on the real-time relative position, and send it to the micro-motion platform control module; the micro-motion platform control module is also used to control the micro-motion platform 200 to move according to the real-time second target displacement.

In order to improve the focusing efficiency, the focusing control device also includes a signal judgment module, which is used to determine whether the focusing device 300 is working normally before obtaining the first target displacement required for the macro-motion platform 100 to move, and whether the focusing signal of the focusing device 300 is within the working range. If so, the focusing device 300 is triggered to execute the acquisition of the first target displacement of the macro-motion platform 100. If not, an error is reported and the process ends, which is convenient for timely maintenance and inspection.

It should be noted that the error reporting here can be connected to a display and/or an alarm through the judgment module to display error information and/or generate sound error reporting and/or emit light error reporting.

In order to obtain whether the focusing device 300 is operating normally in a timely manner, the signal judgment module is also used to determine whether the focusing device 300 is operating normally before obtaining the second target displacement required for the micro-motion platform 200 to move, and whether the focusing signal of the focusing device 300 is within the working range. If so, the focusing device 300 is triggered to execute the acquisition of the second target displacement of the micro-motion platform 200. If not, an error is reported.

In some possible schemes of the present application, the signal judgment module is also used to drive the macro-motion platform 100 to move to the first target position, and then judge whether the focusing device 300 is focused successfully. When the focusing fails, judge whether the stop focusing instruction of the focusing device 300 is valid. If not, judge whether the focusing device 300 is working normally and whether the focus signal of the focusing device 300 is within the working range. If valid, end; when the focusing is successful, judge whether the focus of the focusing device 300 enters a stable state. If the focus enters a stable state, trigger the macro-motion platform control module to execute the locking of the position of the macro-motion platform 100. If the focus does not enter a stable state, judge whether the stop focusing instruction of the focusing device 300 is valid. If not, judge whether the focusing device 300 is working normally and whether the focus signal of the focusing device 300 is within the working range. If valid, end.

The present application locks the macro-motion platform 100 to avoid the macro-motion platform 100 from being displaced after the adjustment of the macro-motion platform 100 is completed, thereby preventing the accuracy of the adjustment from being affected.

It should be noted that the position of the macro-motion platform 100 can be locked by a macro-motion driver, or other locking structures that restrict the movement of the macro-motion platform 100 can be provided.

The signal judgment module is also used to judge whether the stop command of the focusing device 300 is valid after the micro-motion platform 200 moves to the second target position. If it is valid, it ends; if it is invalid, it further judges whether the focusing device 300 works normally and whether the focusing signal of the focusing device 300 is within the working range. If so, it reports an error; if not, it triggers the micro-motion platform control module to control the micro-motion platform 200 to move to the second target position.

After the signal determination module moves to the second target position through the micro-motion platform 200 , it determines the focusing status of the focusing device 300 and triggers the execution of the corresponding status in time, thereby further improving the efficiency.

In order to improve the focusing accuracy, the focusing control device further includes an initialization control module, which is used to control the micro-motion platform 200 to move to an initial position and control the macro-motion platform 100 to move to an initial focusing position.

It should be noted that, in addition to moving along the first direction, the macro motion platform 100 can also move along a second direction perpendicular to the first direction. Of course, it can also move along the first direction, the second direction and the third direction respectively, and the second direction intersects with the third direction and is perpendicular to the first direction, so as to achieve adjustment of different position points in space. Take the first direction as the Z direction, the second direction as the X direction, and the third direction as the Y direction as an example.

In the third aspect, referring to Figure 3, a schematic diagram of the structure of an electronic device suitable for implementing some embodiments of the present application is shown. The electronic device shown in Figure 3 is only an example and should not bring any limitation to the functions and scope of use of the embodiments of the present application.

As shown in FIG3 , the electronic device includes a processor, a memory and a communication bus, wherein the processor and the memory are connected to each other through the communication bus, and the processor can be a central processing unit (CPU), an application-specific integrated circuit (ASIC), a digital signal processor (DSP), an application-specific integrated circuit (ASIC), a field-programmable gate array (FPGA) or other programmable logic devices, etc.

The memory may include a program storage area and a data storage area, wherein the program storage area may store an operating system and an application required for at least one function (such as a focus function, etc.); the data storage area may store data created during the use of the computer, such as a first target displacement amount of the macro-motion platform 100, a second target displacement amount of the micro-motion platform 200, and the like.

In addition, the memory may include a high-speed random access memory, and may also include a non-volatile memory, such as at least one disk storage device or other volatile solid-state storage device. The processor may call a program stored in the memory.

The memory is used to store one or more programs, and the program may include a program code, and the program code includes computer operation instructions. In the embodiment of the present application, the memory at least stores and executes instructions for the focus control method in Example 1.

In a fourth aspect, an embodiment of the present application provides a processor, which is used to run a program, wherein the program, when running, implements the focus control method described in the above method embodiments.

In a fifth aspect, the present application provides a computer-readable medium having a computer program stored thereon, wherein when the computer program is executed by a processor, the focus control method described in the above method embodiment is implemented.

In a sixth aspect, the present application provides a computer program product which, when executed on an electronic device, enables the electronic device to implement the focus control method as described in the above method embodiment.

In the seventh aspect, please refer to FIG. 4 and FIG. 5 , the present application provides a focusing platform system 1000 , wherein the focusing platform system 1000 includes a macro-motion platform 100 , a micro-motion platform 200 , and a focusing device 300 .

The focusing device 300 is mounted on the micro-motion platform 200, and realizes position adjustment along the first direction with the micro-motion platform 200. The macro-motion platform 100 is used to carry the sample 500 to be detected. Specifically, the macro-motion platform 100 can fix the sample 500 to be detected on the macro-motion platform 100 by adsorption or the like.

Of course, the micro-motion platform 200 can also be installed on the macro-motion platform 100, and the micro-motion platform 200 is used to carry the sample to be detected 500. In this embodiment, the focusing device 300 is installed on the micro-motion platform 200, and the macro-motion platform 100 is used to carry the sample to be detected 500.

The micro-motion platform 200 and the macro-motion platform 100 can be moved along the first direction respectively to adjust the distance between the sample 500 to be detected and the focusing device 300, thereby achieving focusing.

The focusing platform system 1000 provided in the present application combines macro-motion and micro-motion together to achieve high-stability, fast, large-stroke and high-precision optical focusing positioning movement.

In some possible solutions of the present application, the macro motion platform 100 includes a macro motion driver and a first motion platform 101, wherein the first motion platform 101 is fixed on the macro motion driver, and the first motion platform 101 is driven by the macro motion driver to move along a first direction.

In order to realize the spatial movement of the macro motion platform 100, the macro motion platform 100 further includes a fixed seat 102, a first moving seat 103, a second moving seat 104, a first driver and a second driver. The fixed seat 102, the first moving seat 103, the second moving seat 104 and the first moving platform 101 are stacked in sequence along the first direction, and the first moving seat 103 moves along the second direction relative to the fixed seat 102, and the second moving seat 104 moves along the third direction relative to the first moving seat 103. The second direction intersects with the third direction, and both are arranged perpendicular to the first direction.

The first moving seat 103 can not only serve as a moving end that moves in the second direction relative to the fixed seat 102, but also serves as a fixed end of the second moving seat 104 that moves along the third direction. Compared with the technical solution of stacking components in two directions, the structure is simplified, which is equivalent to reducing the application volume of the macro motion platform 100.

The macro-motion driver includes a macro-motion stator and a macro-motion mover, wherein the macro-motion stator is mounted on the second motion seat 104, and the macro-motion mover is mounted on the first motion stage 101. Of course, the macro-motion mover can also be mounted on the second motion seat 104, and the macro-motion stator is mounted on the first motion stage 101. The macro-motion stator and the macro-motion mover cooperate to drive the first motion stage 101 to move along the first direction.

The first driver includes a first stator and a first rotor, wherein the first stator is mounted on the first moving seat 103, and the first rotor is mounted on the fixed seat 102. Of course, it can also be configured that the first stator is mounted on the fixed seat 102, and the first rotor is mounted on the first moving seat 103. The first stator and the first rotor cooperate to drive the first moving seat 103 to move relative to the fixed seat 102 along the second direction.

The second driver includes a second stator and a second rotor, wherein the second stator is mounted on the second moving seat 104, and the second rotor is mounted on the first moving seat 103. Of course, it can also be configured that the second stator is mounted on the first moving seat 103, and the second rotor is mounted on the second moving seat 104. The second stator and the second rotor cooperate to drive the second moving seat 104 to move relative to the first moving seat 103 along the third direction.

In order to reduce the friction force when the first moving seat 103 moves relative to the fixed seat 102, a first slide rail is provided on the first moving seat 103. Of course, the first slide rail can also be provided on the fixed seat 102. It can be understood that the first slide rail is extended along the second direction.

In order to reduce the friction force when the second moving seat 104 moves relative to the first moving seat 103, a second slide rail is provided on the first moving seat 103. Of course, the second slide rail can also be provided on the second moving seat 104. It can be understood that the second slide rails are all extended along the third direction.

In order to detect the position of the first moving seat 103, the present application discloses that the macro-motion platform 100 also includes a first position detection device, and the first position detection device includes a first scale member and a first displacement collector. The first scale member is installed on the first stator, and the first displacement collector is installed on the first mover. Alternatively, the first scale member is installed on the first mover, and the first displacement collector is installed on the first stator. The first scale member is extended along the second direction. After the first mover moves relative to the first stator, the first displacement collector can read the value on the first scale member to obtain the displacement value of the first mover, and then obtain the displacement of the first moving seat 103.

In order to detect the position of the second moving seat 104, the present application discloses that the macro-motion platform 100 also includes a second position detection device, the second position detection device includes a second scale member and a second displacement collector, the second scale member is installed on the second stator, and the second displacement collector is installed on the second mover, or the second scale member is installed on the second mover, and the second displacement collector is installed on the second stator, the second scale member is extended along a third direction, after the second mover moves relative to the second stator, the second displacement collector can read the value on the second scale member to obtain the displacement value of the second mover, and then obtain the displacement of the second moving seat 104.

In order to detect the position of the first moving table 101, the present application discloses that the macro-motion platform 100 also includes a third position detection device, and the third position detection device includes a third scale member and a third displacement collector. The third scale member is installed on the macro-motion stator, and the third displacement collector is installed on the macro-motion mover. Alternatively, the third scale member is installed on the macro-motion mover, and the third displacement collector is installed on the macro-motion stator. The third scale member is extended along the first direction. After the macro-motion mover moves relative to the macro-motion stator, the third displacement collector can read the value on the third scale member to obtain the displacement value of the macro-motion mover, and then obtain the displacement of the first moving table 101.

In order to further reduce the volume of the macro-motion platform 100, a sink for installing the first motion platform 101 is provided on the second motion seat 104, thereby reducing the overall height of the macro-motion platform 100.

In order to adjust different angles of the sample 500 to be detected, the macro motion platform 100 also includes a rotating shaft 105 and a sample platform 106. The rotating shaft 105 is rotatably mounted on the first motion platform 101, and the sample platform 106 is mounted on the rotating shaft 105. The sample platform 106 is used to load the sample 500 to be detected.

In order to facilitate driving the rotating shaft 105 to rotate, the present application discloses that the macro-motion platform 100 also includes a rotating driver for driving the rotating shaft 105 to rotate. Specifically, the rotating driver includes a rotating stator and a rotating rotor. The rotating stator is fixed on the first moving platform 101, and the rotating rotor is fixed on the rotating shaft 105. Of course, it can also be arranged that the rotating stator is fixed on the rotating shaft 105, and the rotating rotor is fixed on the first moving platform 101.

In order to detect the rotation angle of the rotating shaft 105, the present application discloses that the macro-motion platform 100 also includes an angle detection device, and the angle position detection device includes an angle scale and an angle collector. The angle scale is installed on the rotating stator, and the angle collector is installed on the rotating mover. Alternatively, the angle scale is installed on the rotating mover, and the angle collector is installed on the rotating stator. The angle scale is arranged around the rotating shaft 105 with a point on the axis line of the rotating shaft 105 as the center of the circle. After the rotating mover moves relative to the rotating stator, the angle collector can read the value on the angle scale to obtain the displacement value of the rotating mover, and then obtain the rotation angle of the rotating shaft 105.

The first displacement collector, the second displacement collector, the third displacement collector and the angle collector are but not limited to photoelectric sensor readers, and the first scale device, the second scale device, the third scale device and the angle scale are but not limited to grating rulers.

In order to facilitate the installation of the fine-tuning platform, the focusing platform system 1000 also includes a bracket 400, the bracket 400 is covered on the fixing seat 102, and the fine-tuning platform is installed on the bracket 400, and the focusing device 300 is installed on the fine-tuning platform.

In an eighth aspect, the present application provides a detection device, including an optical system and a focusing platform system 1000 corresponding to the optical system, such as the one in the above embodiment.

Specifically, the detection device is used for gene detection, and the focusing platform system 1000 supports the gene detection sample and focuses the position of the gene detection sample relative to the optical system.

Ninthly, the present application provides a focusing method for a nucleic acid detection system, including: adjusting the macro-motion platform 100 to drive the sequencing chip to move relative to the objective lens, so that the objective lens receives the optical signal of the sequencing chip; determining the target focusing position of the macro-motion platform 100 along the optical axis direction of the objective lens based on the optical signal and driving the macro-motion platform 100 to move a first target displacement amount along the optical axis direction to the first target position; obtaining an error value between the first target position and the target focusing position; and obtaining a second target displacement amount based on the error value and driving the micro-motion platform 200 to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement amount.

The objective lens is mounted on the micro-motion platform 200, and the macro-motion platform 100 is used to carry the sequencing chip; or, the micro-motion platform 200 is mounted on the macro-motion platform 100, and the micro-motion platform 200 is used for the sequencing chip.

It should be noted that the sequencing chip can be adsorbed on the micro-motion platform 200 by negative pressure, and can also be fixed on the micro-motion platform 200 by other methods.

Specifically, the macro-motion platform 100 is a linear motor control platform, and the micro-motion platform 200 is a piezoelectric ceramic control platform. Taking the micro-motion platform 200 installed on the macro-motion platform 100 and used for a sequencing chip as an example, the macro-motion platform 100 realizes a large-stroke rapid adjustment of the position of the sequencing chip, and the micro-motion platform 200 realizes a precise adjustment of the position of the sequencing chip.

In order to facilitate further precise control of the movement of the micro-motion platform 200, the focusing method also includes real-time detection and feedback of the relative position between the objective lens and the sequencing chip during the movement of the micro-motion platform 200 and adjustment of the driving signal of the micro-motion platform 200. The present application achieves rapid and precise position adjustment of the micro-motion platform 200 by real-time adjustment of the driving signal driving the micro-motion platform 200.

In some embodiments, before driving the macro-motion platform 100 to move to the first target displacement and before driving the micro-motion platform 200 to adjust the relative position between the objective lens and the sequencing chip, it also includes: judging whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If so, executing subsequent steps, if not, reporting an error.

It should be noted that the normal operation of the objective lens here means that the objective lens transmits signals normally, that is, both the received signals and the sent signals are normal.

Whether the focus signal of the objective lens is within the working range means that the image captured by the objective lens can be switched between clear and blurred.

The focusing signal of the objective lens includes difference, sum and difference and division signals.

It should be noted that the two-pixel photodiode outputs two light signals according to the photosensitivity of the two pixels, the difference of the two light signals is the difference signal, the sum of the two light signals is the sum signal, and the ratio of the difference of the two light signals to the sum of the two light signals is the difference sum division signal.

Furthermore, the present application discloses that after driving the macro-motion platform 100 to move to the first target position, it also includes: judging whether the focusing is successful; if the focusing fails, checking whether the focus instruction of stopping the objective lens is valid, if it is valid, ending, if not, returning to the step corresponding to the macro-motion platform 100 of judging whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range; if the focusing is successful, judging whether the focus of the objective lens enters a stable state, if the focus does not enter a stable state, returning to the step of checking whether the stop focus instruction is valid, and if the focus enters a stable state, locking the macro-motion platform 100 along the position.

It should be noted that the end here refers to stopping the focusing work of the entire nucleic acid detection system, so that manual maintenance can be carried out, etc., avoiding invalid focusing and further saving time.

The present application locks the movement of the macro-motion platform 100 to avoid the macro-motion platform 100 being displaced after the adjustment of the macro-motion platform 100 to affect the accuracy of the adjustment.

It should be noted that the position of the macro-motion platform 100 can be locked by a macro-motion driver, or other locking structures that restrict the movement of the macro-motion platform 100 can be provided.

Furthermore, after driving the micro-motion platform 200 to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement, the method also includes: checking whether the stop focus instruction is valid, if so, ending, and if not, returning to the step of determining whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range corresponding to the micro-motion platform 200.

In order to improve the focusing accuracy, the present application discloses that before starting the focusing device 300 , the process further includes: initializing the micro-motion platform 200 , and driving the macro-motion platform 100 to enter an initial focusing position.

Specifically, initialization of the micro-motion platform 200 refers to the micro-motion platform 200 moving to an initial position, and entry of the macro-motion platform 100 into an initial focusing position refers to the macro-motion platform 100 moving to an initial position.

In a tenth aspect, the present application provides a nucleic acid detection system, wherein the nucleic acid detection system includes a sequencing chip, an optical system, a macro-motion platform 100, a micro-motion platform 200, and a focus control mechanism.

The optical system includes an objective lens for receiving optical signals from the sequencing chip.

The objective lens is mounted on the micro-motion platform 200, and the macro-motion platform 100 is used to carry the sequencing chip. Of course, the micro-motion platform 200 can also be mounted on the macro-motion platform 100, and the micro-motion platform 200 is used to carry the sequencing chip.

The focus control mechanism is respectively associated with the macro-motion platform 100 and the micro-motion platform 200. Specifically, the focus control mechanism is used to: adjust the macro-motion platform 100 to drive the sequencing chip to move relative to the objective lens, so that the objective lens receives the optical signal of the sequencing chip; determine the target focus position of the macro-motion platform 100 along the optical axis of the objective lens based on the optical signal and drive the macro-motion platform 100 to move a first target displacement amount along the optical axis to the first target position; obtain the error value between the first target position and the target focus position; and obtain the second target displacement amount based on the error value and drive the micro-motion platform 200 to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement amount.

Specifically, the focus control mechanism specifically includes a micro-motion platform control device and a macro-motion platform control device. The macro-motion platform control device is used to control the movement of the macro-motion platform 100 to adjust the macro-motion platform 100 to drive the sequencing chip to move relative to the objective lens so that the objective lens receives the optical signal of the sequencing chip. The micro-motion platform control device is used to obtain the error value between the first target position and the target focus position, obtain the second target displacement based on the error value, and drive the micro-motion platform 200 to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement.

Furthermore, the micro-motion platform control device of the focus control mechanism is also used to detect and feedback the relative position between the objective lens and the sequencing chip in real time during the movement of the micro-motion platform 200, and drive the micro-motion platform 200 to move according to the relative position.

In order to improve the focusing efficiency, the nucleic acid detection system also includes a signal judgment device, which is used to determine whether the objective lens is working normally and whether the focusing signal of the objective lens is within the working range before obtaining the first target displacement required for the macro-motion platform 100 to move. If so, the objective lens is triggered to execute the acquisition of the first target displacement of the macro-motion platform 100. If not, an error is reported.

The error reporting here can be achieved by connecting a display and/or an alarm, etc., to display error information and/or generate sound error reporting and/or emit light error reporting.

It should be noted that the normal operation of the objective lens here means that the objective lens transmits signals normally, that is, both the received signals and the sent signals are normal.

Whether the focus signal of the objective lens is within the working range means that the image captured by the objective lens can be switched between clear and blurred.

The focus signal includes a sum signal and a difference and division signal.

It should be noted that the two-pixel photodiode outputs two light signals according to the photosensitivity of the two pixels, the sum of the two light signals is the sum signal, and the ratio of the difference between the two light signals and the sum of the two light signals is the difference sum division signal.

The signal judgment device is also used to judge whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range before obtaining the second target displacement required for the micro-motion platform 200 to move. If so, the objective lens is triggered to execute the acquisition of the second target displacement of the micro-motion platform 200. If not, an error is reported.

Furthermore, the signal judgment device is also used to judge whether the objective lens is focused successfully after the macro-motion platform 100 moves to the first target position. When the focusing fails, it is judged whether the stop focusing instruction of the objective lens is valid. If not, it is judged whether the objective lens works normally and whether the focus signal of the objective lens is within the working range. If it is valid, it ends; when the focusing is successful, it is judged whether the focus of the objective lens enters a stable state. If the focus enters a stable state, the focus control mechanism is triggered to lock the position of the macro-motion platform 100 along the first direction. If the focus does not enter a stable state, it is judged whether the stop focusing instruction of the objective lens is valid. If not, it is judged whether the objective lens works normally and whether the focus signal of the objective lens is within the working range. If it is valid, it ends.

The end here refers to stopping the focusing work of the entire nucleic acid detection system, so that manual maintenance can be carried out, etc., avoiding invalid focusing and further saving time.

Furthermore, the signal judgment device is also used to judge whether the stop command of the objective lens is valid after the micro-motion platform 200 moves to the second target position. If it is valid, it ends; if it is invalid, it judges whether the objective lens works normally and whether the focus signal of the objective lens is within the working range. If so, it reports an error; if not, it triggers the focus control mechanism to control the micro-motion platform 200 to move to the second target position.

In order to improve the focusing accuracy, the nucleic acid detection system also includes an initialization control device, which is used to control the micro-motion platform 200 to move to an initial position and control the macro-motion platform 100 to move to an initial focusing position.

It should be noted that, for the convenience of description, only the parts related to the relevant application are shown in the drawings. In the absence of conflict, the embodiments and features in the embodiments of this application can be combined with each other.

It should be understood that the "system", "device", "unit" and/or "module" used in this application is a method for distinguishing different components, elements, parts, parts or assemblies at different levels. However, if other words can achieve the same purpose, the word can be replaced by other expressions.

As shown in this application and claims, unless the context clearly indicates an exception, the words "a", "an", "a kind" and/or "the" do not refer to the singular, but also include the plural. Generally speaking, the terms "include" and "comprise" only indicate the inclusion of clearly identified steps and elements, and these steps and elements do not constitute an exclusive list. The method or device may also include other steps or elements. The elements defined by the sentence "includes a..." do not exclude the existence of other identical elements in the process, method, commodity or device that includes the elements.

In the description of the embodiments of the present application, unless otherwise specified, "/" means or, for example, A/B can mean A or B; "and/or" in this article is only a description of the association relationship of associated objects, indicating that there can be three relationships, for example, A and/or B can mean: A exists alone, A and B exist at the same time, and B exists alone. In addition, in the description of the embodiments of the present application, "multiple" means two or more than two.

In the following, the terms "first" and "second" are used for descriptive purposes only and are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features shown. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features.

Flowcharts are used in the present application to illustrate the operations performed by the system according to the embodiments of the present application. It should be understood that the preceding or following operations are not necessarily performed accurately in order. On the contrary, the various steps may be processed in reverse order or simultaneously. At the same time, other operations may also be added to these processes, or one or more operations may be removed from these processes.

The above description is only a preferred embodiment of the present application and an explanation of the technical principles used, and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. The scope of application involved in the present application is not limited to the technical solution formed by a specific combination of the above-mentioned technical features, but should also cover other technical solutions formed by any combination of the above-mentioned technical features or their equivalent features without departing from the above-mentioned application concept. For example, the above-mentioned features are replaced with the technical features with similar functions disclosed in this application (but not limited to) to form a technical solution.

Claims (31)

A focus control method, characterized in that it is used for a focus platform system, the focus platform system includes a focus device, a micro-motion platform and a macro-motion platform; the focus device is installed on the micro-motion platform, and the macro-motion platform carries a sample to be detected; or the micro-motion platform is installed on the macro-motion platform, and the micro-motion platform carries a sample to be detected; the focus control method includes: Starting the focusing device, adjusting the macro-motion platform to drive the sample to be detected to move relative to the focusing device, so that the focusing device receives the optical signal of the sample to be detected; Determine a target focus position of the macro-motion platform along the optical axis direction of the focusing device based on the optical signal and drive the macro-motion platform to move a first target displacement amount along the optical axis direction to a first target position; Obtaining an error value between the first target position and the target focus position; A second target displacement is acquired based on the error value, and the micro-motion platform is driven according to the second target displacement to adjust the relative position between the focusing device and the sample to be detected. The focus control method according to claim 1, wherein the macro-motion platform is a linear motor control platform; The micro-motion platform is a piezoelectric ceramic control platform. The focus control method according to claim 1 is characterized in that the focus control method further comprises: detecting and feeding back in real time the relative position between the focus device and the sample to be detected during the movement of the micro-motion platform and adjusting the driving signal of the micro-motion platform. The focus control method as described in claim 1 is characterized in that before driving the macro-motion platform to move to the first target displacement and before driving the micro-motion platform to adjust the relative position between the focus device and the sample to be detected, it also includes: judging whether the focus device is working normally and whether the focus signal of the focus device is within the working range, if so, executing subsequent steps, if not, reporting an error. The focus control method according to claim 4, wherein the normal operation of the focus device is specifically that the focus device transmits a normal signal; The focusing signal of the focusing device includes a sum signal and a difference and division signal. The focus control method according to claim 4, characterized in that after driving the macro-motion platform to move to the first target position, the method further comprises: Determine whether the focusing is successful; If the focusing fails, check whether the focusing instruction to stop the focusing device is valid. If it is valid, end the process. If it is invalid, return to the step corresponding to the macro-motion platform to determine whether the focusing device works normally and whether the focusing signal of the focusing device is within the working range. If the focusing is successful, it is determined whether the focusing of the focusing device has entered a stable state. If the focusing has not entered a stable state, the process returns to the step of checking whether the stop focusing instruction is valid. If the focusing has entered a stable state, the macro motion platform is locked. The focus control method as described in claim 4 is characterized in that after driving the micro-motion platform to adjust the relative position between the focus device and the sample to be detected according to the second target displacement, it also includes: checking whether the stop focus instruction is valid, if so, ending, if not, returning to the step corresponding to the micro-motion platform to determine whether the focus device is working normally and whether the focus signal of the focus device is within the working range. The focus control method according to any one of claims 1 to 7, characterized in that before starting the focus device, it also includes: initializing the micro-motion platform and driving the macro-motion platform to enter an initial focus position. The focus control method according to any one of claims 1 to 7, wherein the sample to be detected is a tissue sample. The focus control method according to claim 9, wherein the sample to be detected is a nucleic acid tissue library. A focus control device, characterized in that it is applied to a focus platform system, wherein the focus platform system comprises a focus device, a micro-motion platform and a macro-motion platform; the focus device is mounted on the micro-motion platform, and the macro-motion platform carries a sample to be detected; or the micro-motion platform is mounted on the macro-motion platform, and the micro-motion platform carries a sample to be detected; The focus control device further comprises: a focus module connected to the focus device, a micro-motion platform control module connected to the micro-motion platform, and a macro-motion platform control module connected to the macro-motion platform; The macro-motion platform drives the sample to be detected to move relative to the focusing device, so that the focusing device receives the optical signal of the sample to be detected, and the focusing module obtains the initial displacement of the macro-motion platform according to the optical signal, and sends the initial displacement to the macro-motion platform control module; The macro-motion platform control module controls the macro-motion platform to move to a position where the focusing device receives the optical signal of the sample to be detected; The focusing module further acquires a first target displacement amount required for the macro-motion platform to move to a target focusing position along the optical axis direction of the focusing device, and sends the first target displacement amount to the macro-motion platform control module; The macro-motion platform control module controls the macro-motion platform to move to a first target position based on the first target displacement; The focusing module obtains an error value between the first target position and the target focusing position, obtains a second target displacement amount required for the micro-motion platform to move based on the error value, and sends the second target displacement amount to the micro-motion platform control module; The micro-motion platform control module controls the movement of the micro-motion platform to adjust the relative position between the focusing device and the sample to be detected. The focus control device according to claim 11, characterized in that during the movement of the micro-motion platform, the focus module obtains the relative position between the focus device and the sample to be detected in real time, obtains the real-time displacement of the second target based on the real-time relative position, and sends it to the micro-motion platform control module; The micro-motion platform control module controls the micro-motion platform to move according to the real-time second target displacement. The focus control device according to claim 11, further comprising a signal determination module; Before the focusing module obtains the first target displacement amount required for the macro-motion platform to move, the signal judgment module determines whether the focusing device works normally and whether the focusing signal of the focusing device is within the working range. If so, the focusing device is triggered to execute the acquisition of the first target displacement amount of the macro-motion platform. If not, an error is reported. Before the focusing module obtains the second target displacement amount required for the micro-motion platform to move, the signal judgment module 2 determines whether the focusing device is working normally and whether the focusing signal of the focusing device is within the working range. If so, the focusing device is triggered to execute the acquisition of the second target displacement amount of the micro-motion platform. If not, an error is reported. The focus control device according to claim 11, characterized in that after the macro platform moves to the first target position, the signal judgment module further judges whether the focus device is focused successfully, When focusing fails, determining whether the stop focusing instruction of the focusing device is valid; if not, determining whether the focusing device works normally and whether the focusing signal of the focusing device is within the working range; if valid, ending; When the focusing is successful, determine whether the focus of the focusing device has entered a stable state. If the focus has entered a stable state, trigger the macro-motion platform control module to lock the macro-motion platform. If the focus has not entered a stable state, determine whether the focus stop instruction of the focusing device is valid. If not, determine whether the focusing device is working normally and whether the focus signal of the focusing device is within the working range. If valid, end. The focus control device as described in claim 11 is characterized in that, after the micro-motion platform moves to the second target position, the signal judgment module further judges whether the stop instruction of the focus device is valid, and if so, ends; if not, judges whether the focus device works normally and whether the focus signal of the focus device is within the working range; if so, reports an error; if not, triggers the micro-motion platform control module to control the micro-motion platform to move to the second target position. The focus control device according to any one of claims 11 to 15, further comprising an initialization control module; The initialization control module controls the micro-motion platform to move to an initial position, and controls the macro-motion platform to move to an initial focusing position. An electronic device, characterized in that it comprises a processor, a memory and a communication bus, wherein the processor and the memory are connected to each other via the communication bus, and the memory stores at least one or more programs; The processor calls the program stored in the memory and executes the focus control method according to any one of claims 1 to 10. A computer-readable medium, characterized in that a computer program is stored thereon, wherein when the computer program is executed by a processor, the focus control method according to any one of claims 1 to 10 is implemented. A focusing method for a nucleic acid detection system, characterized by comprising: Adjusting the macro-motion platform to drive the sequencing chip to move relative to the objective lens, so that the objective lens receives the optical signal of the sequencing chip; Determine a target focus position of the macro-motion platform along the optical axis direction of the objective lens based on the optical signal and drive the macro-motion platform to move a first target displacement amount along the optical axis direction to a first target position; Acquire an error value between the first target position and the target focus position; and Acquire a second target displacement based on the error value and drive the micro-motion platform to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement; The objective lens is mounted on the micro-motion platform, and the macro-motion platform is used to carry the sequencing chip; or the micro-motion platform is mounted on the macro-motion platform, and the micro-motion platform is used for the sequencing chip. The focusing method of the nucleic acid detection system according to claim 19, characterized in that the macro-motion platform is a linear motor control platform; The micro-motion platform is a piezoelectric ceramic control platform. The focusing method as described in claim 19 is characterized in that the focusing method further includes: detecting and feeding back the relative position between the objective lens and the sequencing chip in real time during the movement of the micro-motion platform and adjusting the driving signal of the micro-motion platform. The focusing method as described in claim 19 is characterized in that before driving the macro-motion platform to move to the first target displacement and before driving the micro-motion platform to adjust the relative position between the objective lens and the sequencing chip, it also includes: judging whether the objective lens is working normally and whether the focusing signal of the objective lens is within the working range, if so, executing subsequent steps, if not, reporting an error. The focusing method according to claim 22, characterized in that after driving the macro-motion platform to move to the first target position, the method further comprises: Determine whether the focusing is successful; If the focusing fails, check whether the focusing instruction to stop the objective lens is valid, if it is valid, end, if not, return to the step corresponding to the macro-motion platform to determine whether the objective lens works normally and whether the focusing signal of the objective lens is within the working range; If the focusing is successful, it is determined whether the focusing of the objective lens enters a stable state. If the focusing does not enter a stable state, the process returns to the step of checking whether the stop focusing instruction is valid. If the focusing enters a stable state, the macro motion platform is locked. The focusing method as described in claim 22 is characterized in that after driving the micro-motion platform to adjust the relative position between the objective lens and the sequencing chip according to the second target displacement, it also includes: checking whether the stop focusing instruction is valid, if so, ending, if not, returning to the step corresponding to the micro-motion platform to determine whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. The focusing method according to any one of claims 19 to 24, characterized in that before starting the focusing device, it also includes: initializing the micro-motion platform and driving the macro-motion platform to enter an initial focusing position. A nucleic acid detection system, comprising a sequencing chip and an optical system, wherein the optical system comprises an objective lens for receiving an optical signal of the sequencing chip, wherein the nucleic acid detection system further comprises a macro-motion platform and a micro-motion platform; The objective lens is mounted on the micro-motion platform, and the macro-motion platform is used to carry the sequencing chip; or the micro-motion platform is mounted on the macro-motion platform, and the micro-motion platform is used to carry the sequencing chip. The nucleic acid detection system further includes a focus control mechanism associated with the macro-motion platform and the micro-motion platform, and the focus control mechanism performs: Adjusting the macro-motion platform to drive the sequencing chip to move relative to the objective lens, so that the objective lens receives the optical signal of the sequencing chip; Determine a target focus position of the macro-motion platform along the optical axis direction of the objective lens based on the optical signal and drive the macro-motion platform to move a first target displacement amount along the optical axis direction to a first target position; Acquire an error value between the first target position and the target focus position; and A second target displacement is obtained based on the error value, and the micro-motion platform is driven according to the second target displacement to adjust the relative position between the objective lens and the sequencing chip. The nucleic acid detection system as described in claim 26 is characterized in that during the movement of the micro-motion platform, the focus control mechanism detects and feeds back the relative position between the objective lens and the sequencing chip in real time, and drives the micro-motion platform to move according to the relative position. The nucleic acid detection system according to claim 27, further comprising a signal determination device; Before obtaining the first target displacement amount required for the macro-motion platform to move, the signal judgment device judges whether the objective lens works normally and whether the focus signal of the objective lens is within the working range. If so, the objective lens is triggered to execute the acquisition of the first target displacement amount of the macro-motion platform. If not, an error is reported. Before obtaining the second target displacement amount required for the micro-motion platform to move, the signal judgment device judges whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If so, the objective lens is triggered to execute the acquisition of the second target displacement amount of the micro-motion platform movement. If not, an error is reported. The nucleic acid detection system according to claim 26, characterized in that after the macro-motion platform moves to the first target position, the signal judgment device further judges whether the objective lens is focused successfully, When focusing fails, judging whether the stop focusing instruction of the objective lens is valid, if not valid, judging whether the objective lens works normally and whether the focus signal of the objective lens is within the working range, if valid, ending; When the focusing is successful, determine whether the focus of the objective lens enters a stable state. If the focus enters a stable state, trigger the focus control mechanism to lock the macro-motion platform. If the focus does not enter a stable state, determine whether the stop focus instruction of the objective lens is valid. If not, determine whether the objective lens works normally and whether the focus signal of the objective lens is within the working range. If valid, end. The nucleic acid detection system as described in claim 26 is characterized in that after the micro-motion platform moves to the second target position, the signal judgment device further judges whether the stop command of the objective lens is valid. If valid, it ends; if invalid, it judges whether the objective lens is working normally and whether the focus signal of the objective lens is within the working range. If so, it reports an error; if not, it triggers the focus control mechanism to control the micro-motion platform to move to the second target position. The nucleic acid detection system according to any one of claims 26 to 30, further comprising an initialization control device; The initialization control device controls the micro-motion platform to move to an initial position, and controls the macro-motion platform to move to an initial focusing position.

Images