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WO2024191282A1 - Substrat d'aliment pour animaux et procédé de préparation associé - Google Patents

Substrat d'aliment pour animaux et procédé de préparation associé Download PDF

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Publication number
WO2024191282A1
WO2024191282A1 PCT/MY2024/050026 MY2024050026W WO2024191282A1 WO 2024191282 A1 WO2024191282 A1 WO 2024191282A1 MY 2024050026 W MY2024050026 W MY 2024050026W WO 2024191282 A1 WO2024191282 A1 WO 2024191282A1
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WO
WIPO (PCT)
Prior art keywords
grandiflora
leaves
fermented
paste
ranging
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/MY2024/050026
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English (en)
Inventor
Giva KUPPUSAMY
Janaranjani MANOGORAN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sento Biotech Sdn Bhd
Original Assignee
Sento Biotech Sdn Bhd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sento Biotech Sdn Bhd filed Critical Sento Biotech Sdn Bhd
Publication of WO2024191282A1 publication Critical patent/WO2024191282A1/fr
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms

Definitions

  • the present invention relates to feed substrates and method of preparing thereof, in particular the present invention relates to fermented Sesbania grandiflora leaves prepared as a feed substrate for black soldier fly and method of preparing thereof, wherein the fermented Sesbania grandiflora leaves able to develop black soldier fly with desired nutritional content as well as reduced heavy metal content.
  • BSF Black soldier fly
  • BSF larvae can consume a wider range of decomposing organic material at a quicker and more efficient rate than other insects due to the presence of numerous enzymes such as but not limited to leucine arylamidase, a- galactosidase, [3-galactosidase, a-mannosidase and a-fucosidase in their body which enable them to digest glycoproteins, glycolipids and carbohydrates.
  • enzymes such as but not limited to leucine arylamidase, a- galactosidase, [3-galactosidase, a-mannosidase and a-fucosidase in their body which enable them to digest glycoproteins, glycolipids and carbohydrates.
  • BSF larvae are fed with organic wastes such as kitchen waste and agricultural waste.
  • organic wastes such as kitchen waste and agricultural waste.
  • these wastes used for BSF rearing often result in the BSF having high heavy metal content which may pass through the food chain as animal feed and eventually cause toxicity to humans.
  • Sesbania grandiflora S. grandiflora
  • the leaves of S. grandiflora are known for having high content of essential amino acids, minerals, vitamins and other active compounds.
  • the present invention relates to a method of preparing fermented S. grandiflora leaves, wherein the method comprising the steps of (i) adding a solvent to S. grandiflora leaves in a ratio ranging between 0.5:1 by weight per volume to 1 :1 by weight per volume to produce S. grandiflora leaves mixture, (ii) grinding the S. grandiflora leaves mixture obtained from step (i) for a time duration ranging between 5 minutes to 10 minutes to produce S. grandiflora leaves paste, (iii) heating the S. grandiflora leaves paste obtained from step (ii) at a temperature ranging between 80 s C to 100 s C for a time period ranging between 3 minutes to 5 minutes to produce heated S.
  • step (iv) cooling down the heated S. grandiflora leaves paste obtained from step (iii) at a temperature ranging between 28 S C to 35 S C for a time period ranging between 15 minutes to 45 minutes to produce cooled S. grandiflora leaves paste, (v) inoculating Lactobacillus acidophilus into the cooled S. grandiflora leaves paste to produce inoculated S. grandiflora leaves and (vi) covering the inoculated S. grandiflora leaves in a container at a temperature ranging between 29 S C to 33 S C for a time period ranging between 72 hours to 96 hours to produce the fermented S. grandiflora leaves. Also, the present invention relates to fermented S.
  • the present invention relates to fermented S. grandiflora leaves obtained by the method as described above which includes at least 30.5% dry matter of protein, at least 0.8% dry matter of lipid and at least 9% dry matter of ash. Further, the present invention relates to fermented S. grandiflora leaves obtained by the method as described above which includes 72% of essential amino acid and 28% of non-essential amino acid. Still further, the present invention relates to a feed substrate comprises fermented S. grandiflora leaves obtained by the method as described above.
  • the present invention relates to feed substrates and method of preparing thereof, in particular the present invention relates to fermented Sesbania grandiflora leaves prepared as a feed substrate for black soldier fly and method of preparing thereof, wherein the fermented Sesbania grandiflora leaves able to develop black soldier fly with desired nutritional content as well as reduced heavy metal content.
  • First aspect of the present invention discloses a method of preparing fermented S. grandiflora leaves as feed substrate, wherein the method comprising the steps of: i. adding a solvent to S. grandiflora leaves in a ratio ranging between 0.5:1 by weight per volume to 1 :1 by weight per volume to produce S. grandiflora leaves mixture; ii. grinding the S. grandiflora leaves mixture obtained from step (i) for a duration ranging between 5 minutes to 10 minutes, preferably ranging between 5 minutes to 7 minutes, most preferably 5 minutes to produce S. grandiflora leaves paste; iii. heating the S.
  • step (ii) at a temperature ranging between 80 s C to 100 s C, preferably ranging between 90 s C to 100 s C, most preferably 95 S C for a duration ranging between 1 minute to 3 minutes, preferably ranging between 1 minute to 2 minutes, most preferably 1 minute to produce heated S. grandiflora leaves paste; iv. cooling the heated S. grandiflora leaves paste obtained from step (iii) to a temperature ranging between 28 S C to 35 S C, preferably ranging between 28 S C to 32 S C, most preferably 32 S C for a duration ranging between 15 minutes to 45 minutes, preferably ranging between 15 minutes to 30 minutes, most preferably 20 minutes to produce cooled S.
  • step (v) inoculating Lactobacillus acidophilus into the cooled S. grandiflora leaves paste obtained from step (iv) to produce inoculated S. grandiflora leaves; and vi. covering the inoculated S. grandiflora leaves obtained from step (v) in a container at a temperature ranging between 29 S C to 33 S C, preferably ranging between 30 s C to 32 S C, most preferably 31 S C for a duration ranging between 72 hours to 96 hours, preferably ranging between 84 hours to 96 hours, most preferably 96 hours to produce the fermented S. grandiflora leaves of the present invention.
  • the method of preparing fermented S. grandiflora leaves of the present invention includes a step of soaking the fresh S. grandiflora leaves in water for a duration ranging between 2 hours to 24 hours, preferably ranging between 2 hours to 12 hours, most preferably 2 hours, followed by a step of sieving the soaked S. grandiflora leaves to remove water as well as dirt and toxin found on the surface of the leaves using a mesh with a size ranging between 2 mm to 5 mm, preferably ranging between 2 mm to 3 mm, most preferably 2 mm prior to the step (i) of the method mentioned above.
  • the method of the present invention comprises a first step (i) of adding a solvent to S. grandiflora leaves, wherein the solvent is selected from the group consisting of distilled water, sterilized distilled water, ionized water, tap water and mixtures therefrom.
  • the method of the present invention comprises a second step (ii) of grinding the S. grandiflora leaves mixture, wherein the grinding process is carried out using but not limited to a blender and wherein the S. grandiflora leaves paste produced have a particle size ranging between 1 mm to 3 mm and moisture content ranging between 80% to 90% by weight of the S. grandiflora leaves paste.
  • the method of the present invention comprises a third step (iii) of heating the S. grandiflora leaves paste, wherein the heating process is carried out using but not limited to an induction cooker and wherein the heated S. grandiflora leaves paste produced have a moisture content ranging between 80% to 90% by weight of the heated S. grandiflora leaves paste.
  • the method of the present invention comprises a fourth step (iv) of cooling the heated S. grandiflora leaves paste, wherein the cooling process is carried out using but not limited to the method of air drying.
  • the method of the present invention comprises a fifth step (v) of inoculating the Lactobacillus acidophilus into the cooled S. grandiflora leaves paste, wherein the Lactobacillus acidophilus is inoculated in a concentration ranging between 10 5 CFU/g to 10 9 CFU/g, preferably 10 6 CFU/g and wherein the inoculation process is carried out by mixing the Lactobacillus acidophilus to the cooled S. grandiflora leave paste.
  • the method of the present invention comprises a sixth step (vi) of covering the inoculated S. grandiflora leaves in a container, wherein the container can be any container such as but not limited to container made of metal, glass and wood and wherein the walls of the container may have holes for the purpose of aeration. Further, the sixth step (vi) of the method of the present invention is crucial to allow the fermentation process to take place.
  • the fermented S. grandiflora leaves prepared using the method of the present invention has a particle size ranging between 1 mm to 3 mm, moisture content ranging between 80% to 85% by weight of the fermented S. grandiflora leaves and pH ranging between 5.4 to 6.3.
  • Table 1 shows the nutritional content of fermented S. grandiflora leaves of the present invention.
  • the fermented S. grandiflora leaves of the present invention has total amino acid content comprising 72% of essential amino acid and 28% of non-essential amino acid.
  • Second aspect of the present invention discloses the use of the fermented S. grandiflora leaves prepared from the method as disclosed in the first aspect of the present invention as feed substrates for insects such as but not limited to black soldier fly (BSF) and mealworm, especially BSF.
  • BSF black soldier fly
  • the fermented S. grandiflora leaves as feed substrate for insects of the present invention is prepared using the method as disclosed in the first aspect of the present invention.
  • Table 2 displays the nutritional content of BSF prepupae fed with putrefied S. grandiflora leaves and BSF prepupae fed with fermented S. grandiflora leaves, wherein the putrefied S. grandiflora leaves are prepared by allowing the leaves to naturally putrefy for a temperature ranging between 27 S C to 31 S C for a duration ranging between 13 days to 15 days and wherein the fermented S. grandiflora leaves are prepared using the method as disclosed in the first aspect of the present invention.
  • Table 2 Nutritional content of BSF prepupae fed with putrefied S. grandiflora leaves and BSF prepupae fed with fermented S. grandiflora leaves
  • Table 3 shows the total amino acid content of BSF prepupae fed with putrefied S. grandiflora leaves and BSF prepupae fed with fermented S. grandiflora leaves.
  • Table 3 Total amino acid content of BSF prepupae fed with putrefied S. grandiflora leaves and BSF prepupae fed with fermented S. grandiflora leaves The higher the protein and essential amino acid content of BSF prepupae, the better the nutritional content of the BSF prepupae. Based on results obtained in Table 2 and Table 3, it is noticeable that the BSF prepupae fed with fermented S. grandiflora leaves has higher protein and essential amino acid content as compared to the BSF prepupae fed with putrefied S. grandiflora leaves.
  • Table 4 displays the heavy metal content of BSF prepupae fed with putrefied S. grandiflora leaves and BSF prepupae fed with fermented S. grandiflora leaves.
  • Table 4 Heavy metal content of BSF prepupae fed with putrefied S. grandiflora leaves and BSF prepupae fed with fermented S. grandiflora leaves
  • the fermented S. grandiflora leaves prepared in accordance with the method of the present invention is able to overcome the conventional shortcomings since the fermented S. grandiflora leaves of the present invention able to develop BSF with desired nutritional content as well as reduced heavy metal content.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Physiology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Insects & Arthropods (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)

Abstract

L'invention concerne un procédé de préparation de feuilles de S. grandiflora fermentées comprenant les étapes consistant à (i) ajouter un solvant à des feuilles de S. grandiflora, (ii) broyer le mélange de feuilles de S. grandiflora, (iii) chauffer la pâte de feuilles de S. grandiflora, (iv) refroidir la pâte de feuilles de S. grandiflora chauffée, (v) inoculer Lactobacillus acidophilus dans la pâte de feuilles de S. grandiflora refroidie et (vi) recouvrir les feuilles de S. grandiflora inoculées dans un contenant. L'invention concerne des feuilles de S. grandiflora fermentées obtenues par le procédé tel que décrit ci-dessus, lesquelles contiennent au moins 30,5% de matière sèche de protéine, au moins 0,8% de matière sèche de lipide et au moins 9% de matière sèche de cendres. L'invention concerne des feuilles de S. grandiflora fermentées obtenues par le procédé tel que décrit ci-dessus, lesquelles contiennent 72% d'acide aminé essentiel et 28% d'acide aminé non essentiel. Un substrat d'aliment pour animaux contient des feuilles de S. grandiflora fermentées obtenues par le procédé tel que décrit ci-dessus.
PCT/MY2024/050026 2023-03-15 2024-03-15 Substrat d'aliment pour animaux et procédé de préparation associé Pending WO2024191282A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MYPI2023001409 2023-03-15
MYPI2023001409A MY208003A (en) 2023-03-15 2023-03-15 Feed substrate and method of preparing thereof

Publications (1)

Publication Number Publication Date
WO2024191282A1 true WO2024191282A1 (fr) 2024-09-19

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Family Applications (1)

Application Number Title Priority Date Filing Date
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WO (1) WO2024191282A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002066625A1 (fr) * 2001-02-21 2002-08-29 Commonwealth Scientific And Industrial Research Organisation Nouveau gene et ses utilisations pour modifier les qualites du tapis pastoral de cultures
KR20110027735A (ko) * 2011-02-21 2011-03-16 건국대학교 산학협력단 병원성미생물에 대하여 항균활성을 나타내는 식물 또는 그 추출물을 유효성분으로 하는 항생용 조성물
WO2019035143A2 (fr) * 2017-08-16 2019-02-21 Muniyal Ayurvedic Research Centre Miel médicamenteux et son procédé de préparation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002066625A1 (fr) * 2001-02-21 2002-08-29 Commonwealth Scientific And Industrial Research Organisation Nouveau gene et ses utilisations pour modifier les qualites du tapis pastoral de cultures
KR20110027735A (ko) * 2011-02-21 2011-03-16 건국대학교 산학협력단 병원성미생물에 대하여 항균활성을 나타내는 식물 또는 그 추출물을 유효성분으로 하는 항생용 조성물
WO2019035143A2 (fr) * 2017-08-16 2019-02-21 Muniyal Ayurvedic Research Centre Miel médicamenteux et son procédé de préparation

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HAMEED R. SAHUL, PRIYA P. MANJU: "​Thermal Processing and Fermentation Improves the Physicochemical and Functional Properties of Dehydrated Agathi Leaves (Sesbania grandiflora L.)", JOURNAL OF DAIRYING FOODS AND HOME SCIENCES, AGRICULTURAL RESEARCH COMMUNICATION CENTRE, KARNAL,, IN, no. Of, IN , XP093209689, ISSN: 0971-4456, DOI: 10.18805/ajdfr.DR-1602 *
MAZZEO MARIA FIORELLA, LIPPOLIS ROSA, SORRENTINO ALIDA, LIBERTI SARAH, FRAGNITO FEDERICA, SICILIANO ROSA ANNA: "Lactobacillus acidophilus—Rutin Interplay Investigated by Proteomics", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 10, no. 11, US , pages e0142376, XP093209691, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0142376 *

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