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WO2024190607A1 - Appareil de culture et système de culture - Google Patents

Appareil de culture et système de culture Download PDF

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Publication number
WO2024190607A1
WO2024190607A1 PCT/JP2024/008788 JP2024008788W WO2024190607A1 WO 2024190607 A1 WO2024190607 A1 WO 2024190607A1 JP 2024008788 W JP2024008788 W JP 2024008788W WO 2024190607 A1 WO2024190607 A1 WO 2024190607A1
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WO
WIPO (PCT)
Prior art keywords
container
culture
pipe
recovery pipe
culture solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/JP2024/008788
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English (en)
Japanese (ja)
Inventor
政城 遠藤
ホセ ロメル フルーガリダッド マラパスクア
ピーター ヤップ セン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chitose Laboratory Corp
Original Assignee
Chitose Laboratory Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chitose Laboratory Corp filed Critical Chitose Laboratory Corp
Publication of WO2024190607A1 publication Critical patent/WO2024190607A1/fr
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology

Definitions

  • the present invention relates to a culture device and a culture system for culturing aquatic organisms.
  • a culture device for culturing aquatic organisms such as algae, nekton, plankton, and microorganisms requires a container capable of storing a culture solution.
  • Patent Document 1 discloses a culture device for culturing microalgae, which are photosynthetic organisms, that includes a pair of net sections, a number of supports supporting the net sections, and a light-transmitting container disposed between the net sections.
  • this culture device light is irradiated onto the microalgae in the container, and carbon dioxide is supplied to the culture solution in the container, thereby allowing the microalgae to grow by photosynthesis.
  • the culture medium in the container is discharged through a recovery pipe connected to the container, but because the recovery pipe extends horizontally from the container, there is a problem in that it is difficult for the culture medium to flow through the recovery pipe.
  • the objective of the present invention is to solve the above problems and provide a culture device and culture system that can smoothly discharge the culture medium in the container through a recovery pipe connected to the container and efficiently recover the organisms contained in the culture medium.
  • the first invention is a culture device that includes a pair of mesh sections arranged facing each other on the left and right, a support that supports the mesh sections, a container arranged between the two mesh sections, and a recovery pipe connected to the container.
  • the container is capable of storing an organism culture solution.
  • One end of the recovery pipe is inserted into a discharge hole formed in the bottom of the container, and the recovery pipe is inclined so that the other end is positioned lower than the one end.
  • the second invention is a culture system using the culture device, and includes a plurality of the culture devices and a collecting pipe to which the collecting pipes of each of the culture devices are connected.
  • the collecting pipe is disposed below the discharge hole of the container of each of the culture devices.
  • the culture medium in the container flows smoothly through the recovery pipe and is discharged, so that the organisms contained in the culture medium can be recovered efficiently.
  • the culture medium flows down inside the collection pipe toward the collecting collection pipe located below the discharge hole of the container, so that the culture medium can be efficiently collected from a plurality of culture devices.
  • FIG. 1 is an overall configuration diagram of a culture system using a culture device according to an embodiment of the present invention.
  • FIG. 1 is a side cross-sectional view showing a culture device according to an embodiment of the present invention.
  • the culture system 1 is configured by arranging multiple culture devices 10 on the ground.
  • the culture system 1 is installed near a facility that emits carbon dioxide, such as a power plant or factory.
  • the culture device 10 stores culture solution W containing algae in a light-transmitting container 20, irradiates the culture solution W in the container 20 with sunlight, and supplies gas (exhaust gas) containing a large amount of carbon dioxide emitted from the facility to the culture solution W in the container 20, causing the algae to photosynthesize and grow.
  • gas exhaust gas
  • the culture device 10 includes a pair of mesh sections 30, 30 arranged facing each other on the left and right, a support 50 supporting both mesh sections 30, 30, and a container 20 arranged between both mesh sections 30, 30.
  • the culture device 10 also includes an aeration pipe 21 arranged in the container 20, and a recovery pipe 22 connected to the container 20.
  • the length of the culture device 10 in the front-rear direction is, for example, 50 m to 200 m, and the height of the culture device 10 is, for example, 1.5 m.
  • the mesh portion 30 is a rectangular mesh extending in the front-rear direction in a side view.
  • the mesh portion 30 has a rectangular mesh (lattice) formed all over it by metal wires.
  • the material and dimensions of the mesh portion are not limited, but if the wire is made of metal, the diameter of the wire should be approximately 3 mm and the size of each mesh should be approximately 50 mm in length and 100 mm in width, thereby ensuring sufficient strength while reducing the material costs of the mesh portion.
  • the left and right mesh portions 30, 30 are disposed with their inner surfaces facing each other. The distance between the left and right mesh portions 30, 30 in the left-right direction is, for example, 60 mm.
  • a pair of mesh sections 30, 30 is formed by bending a single mesh member into a U-shape, but a pair of mesh sections 30, 30 may be formed by opposing separate members.
  • the portion connecting the pair of mesh sections 30, 30 is formed in a U-shape (arc-shaped), but it may also be formed in a V-shape or a straight line.
  • the support body 50 has a plurality of support columns 51.
  • the support columns 51 are metal members that are erected vertically to the ground.
  • the support columns 51 are erected on the upper surface of a foundation 60 that is installed on the ground.
  • the support body 50 of this embodiment has two columns, one on the left and one on the right.
  • Each column row is made up of a plurality of support columns 51 that are arranged at intervals in the front-rear direction.
  • the support pillars 51 are disposed on the outer surface side of the mesh portion 30. A part of the mesh portion 30 is connected to the support pillars 51, and the connected support pillars 51 position the mesh portion 30 at a predetermined height.
  • the foundation 60 is a member made of precast concrete.
  • the foundation 60 is formed in a rectangular parallelepiped extending in the front-rear direction.
  • the lower part of the foundation 60 is buried in the ground.
  • the foundation 60 may be made of cast-in-place concrete, in which concrete is poured at the installation site, or may be made of steel.
  • the foundation 60 may be a spread foundation placed directly on the ground, or a pile foundation having piles buried in the ground.
  • the container 20 is a flat, bag-like container made of a plastic sheet.
  • the material of the plastic sheet is not limited, but when culturing photosynthetic organisms, a light-transmitting material (e.g., polyethylene terephthalate, polyethylene, polypropylene, etc.) is used.
  • a light-transmitting material e.g., polyethylene terephthalate, polyethylene, polypropylene, etc.
  • the bottom edge and the front and rear edges of the container 20 are closed, and the culture solution W can be stored within the container 20.
  • the entire upper end of the container 20 is open, but the entire upper end of the container 20 or a part of the upper end may be closed.
  • a discharge hole 20a penetrates the lower end of the front edge of the container 20.
  • the lower end of the front edge of the container 20 protrudes forward from between the supports 51, 51 (see Figure 1) at the front end of the culture device 10, and the discharge hole 20a is formed at the tip.
  • the container 20 is formed to be transparent or translucent, allowing light to pass from the outside to the inside. Because the container 20 is thus translucent, sunlight can be irradiated onto the algae in the container 20 by installing the culture device 10 outdoors.
  • the container 20 is placed between the left and right mesh sections 30, 30.
  • culture solution W containing algae is stored in the container 20
  • the container 20 expands in the left and right directions due to water pressure, and the sides of the container 20 are pressed against the inner surfaces of both mesh sections 30, 30. In this way, the container 20 is sandwiched between both mesh sections 30, 30.
  • the container 20 contains an aeration pipe 21 for supplying gas to the culture solution W.
  • the aeration pipe 21 enters the container 20 from outside the culture device 10 through an opening at the top end of the container 20, and extends in the front-to-rear direction within the container 20.
  • the aeration pipe 21 has multiple exhaust holes (not shown).
  • the recovery pipe 22 is a cylindrical pipe body made of resin or steel. As shown in FIG. 2, the recovery pipe 22 passes from the discharge hole 20a above the base 60 to the collecting recovery pipe 3 described below. One end 22a of the recovery pipe 22 is connected to the container 20 from the front side of the culture device 10. The other end 22b of the recovery pipe 22 is connected to the collecting recovery pipe 3 from above.
  • One end 22a of the recovery pipe 22 is inserted into the discharge hole 20a of the container 20.
  • One end 22a of the recovery pipe 22 protrudes into the lower end of the container 20. Additionally, the inner circumferential surface of the discharge hole 20 a of the container 20 is joined to the outer circumferential surface of one end 22 a of the recovery pipe 22 .
  • the recovery pipe 22 is inclined so that the other end 22b (front end) is positioned lower than the other end 22a (rear end). In other words, the recovery pipe 22 extends at an incline downward as it goes forward from the one end 22a on the container 20 side. Moreover, the recovery pipe 22 is bent downward at the other end 22b and extends vertically downward.
  • An on-off valve 22c is provided in the middle of the recovery pipe 22.
  • the on-off valve 22c is closed, the flow of the culture solution W from one end 22a to the other end 22b is blocked.
  • the on-off valve 22c is opened, the culture solution W can flow from the one end 22a to the other end 22b.
  • the on-off valve 22c is opened, the culture solution W in the container 20 is discharged to the outside through the recovery pipe 22.
  • a suction pump (not shown) is disposed downstream of the collecting recovery pipe 3.
  • a culture system 1 using the above-mentioned culture device 10 will be described.
  • a culture system 1 of this embodiment a plurality of culture devices 10 are arranged on the ground as shown in Fig. 1.
  • Each culture device 10 extends in the front-rear direction and is arranged at intervals in the left-right direction.
  • the culture system 1 is provided with an air pipe 2 extending from a facility that emits carbon dioxide (e.g., a power plant, a factory, etc.).
  • the air pipe 2 branches off on the culture system 1 side and is connected to an aeration pipe 21 of each culture device 10. Then, exhaust gas generated within the facility is supplied to the aeration pipe 21 of each culture device 10.
  • the culture system 1 utilizes exhaust gases emitted from facilities such as power plants and factories, thereby reducing the amount of carbon dioxide emitted into the atmosphere and contributing to the prevention of global warming.
  • the culture system 1 is provided with a collecting pipe 3.
  • the collecting pipe 3 is a cylindrical pipe body made of resin or steel, and is formed with an inner diameter larger than that of the collecting pipe 22.
  • One end of the collecting collection pipe 3 extends in the left-right direction in front of each culture device 10.
  • the other end of the collecting collection pipe 3 is connected to a collection container (not shown).
  • the collecting pipe 3 is positioned below the discharge hole 20a of the container 20 of the culture device 10. In other words, the collecting pipe 3 is positioned below the bottom of the container 20.
  • the other end 22b of the collection pipe 22 is connected to the collection pipe 3. As shown in FIG. 1, the culture fluid W stored in the container 20 of each culture device 10 flows into the collection pipe 3 through each collection pipe 22, and is stored in the collection container from the collection pipe 3.
  • each culture device 10 In addition, in the culture system 1, multiple sprinklers (not shown) are installed between each culture device 10. By spraying water from each sprinkler, it is possible to prevent the temperature of the culture solution W in the container 20 from rising, and to adjust the temperature to a level suitable for photosynthesis of the algae.
  • the culture system 1 In order to promote photosynthesis of algae, it is preferable to install the culture system 1 in a low-latitude region. When installing the culture system 1 in such a region, it is preferable to design the lengths of the various pipes taking into account that they will expand and contract due to the influence of the outside temperature.
  • a culture solution W containing algae is stored in the container 20 of each culture device 10 installed outdoors. At this time, the opening/closing valve 22c of the recovery pipe 22 is kept closed.
  • the container 20 is irradiated with sunlight and gas containing a large amount of carbon dioxide is supplied to the culture solution W from the aeration pipe 21 inside the container 20, the algae inside the container 20 produce oxygen and useful components through photosynthesis.
  • the on-off valve 22c of the recovery pipe 22 is opened to allow the culture solution W in the container 20 to flow from the recovery pipe 22 into the collecting recovery pipe 3.
  • the culture solution W stored in the container 20 of each culture device 10 is allowed to flow from the recovery pipe 22 into the collecting recovery pipe 3, and the culture solution W containing the algae is stored in the recovery container.
  • components such as lipids, carbohydrates, and proteins are separated from the algae recovered from the culture solution W, and various products such as biomass fuel and health foods are produced from these components.
  • the culture device 10 of this embodiment includes a pair of left and right mesh sections 30, 30, a support 50 that supports the mesh sections 30, a container 20 disposed between the mesh sections 30, 30, and a recovery pipe 22 connected to the container 20.
  • the container 20 is translucent and capable of storing an algae culture solution W. 2
  • one end 22a of the recovery pipe 22 is inserted into a discharge hole 20a formed in the lower part of the container 20.
  • the recovery pipe 22 is inclined so that the other end 22b is positioned lower than the one end 22a.
  • the recovery pipe 22 is inclined downward as it moves away from the container 20. This allows the culture solution W in the container 20 to flow smoothly through the recovery pipe 22 and be discharged, making it possible to efficiently recover the algae contained in the culture solution W. In addition, the load on the suction pump connected to the collection recovery pipe 3 can be reduced.
  • an on-off valve 22c is provided in the recovery pipe 22, and by opening and closing the on-off valve 22c, it is possible to easily switch between a state in which the culture solution W is stored in the container 20 and a state in which the culture solution W is discharged from the container 20.
  • the culture system 1 of this embodiment includes a plurality of culture devices 10 and a collecting pipe 3 to which the collecting pipes 22 of each culture device 10 are connected.
  • the collecting pipe 3 is disposed below the discharge hole 20a of the container 20 of each culture device 10.
  • the culture solution W flows down inside the collection pipe 22 toward the collection pipe 3 below the discharge hole 20a of the container 20, so the culture solution W can be efficiently collected from multiple culture devices 10.
  • one recovery pipe 22 is connected to the container 20 , but a plurality of recovery pipes 22 may be connected to the container 20 .
  • a culture device 10 for culturing algae as shown in FIG. 1 has been described, but the organisms that can be cultured in the culture device of the present invention are not limited to photosynthetic organisms such as microalgae, and in addition to aquatic photosynthetic organisms other than algae, nekton, plankton, etc. can also be cultured.
  • the container 20 has translucency.
  • the degree of translucency may be appropriately adjusted according to the characteristics of the organism to be cultured, and the container 20 may be transparent so that the inside of the container 20 can be seen, or may be translucent or slightly light-transmitting.
  • the color and material of the container 20 are not limited.
  • the container 20 may be made of a material (color) that absorbs or reflects a specific wavelength range, or a material that has a light-diffusing effect.
  • the container does not have to be light-transmitting.
  • wastewater culture solution
  • the wastewater may be treated by microorganisms (organisms).

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Appareil de culture (10) comprenant : des parties réticulées gauche et droite (30) ; un corps de soutien (50) soutenant les parties réticulées (30) ; un récipient (20) situé entre les deux parties réticulées (30, 30) ; et un tuyau de récupération (22) couplé au récipient (20). Le récipient (20) peut stocker une solution de culture (W). Une extrémité (22a) du tuyau de récupération (22) est insérée dans un trou d'évacuation (20a) dans une partie inférieure du récipient (20). Le tuyau de récupération (22) est incliné de manière à ce qu'une autre de ses extrémités (22b) soit située en dessous de l'une des extrémités (22a). Dans cette configuration, la solution de culture (W) dans le récipient (20) peut être évacuée sans à-coups à travers le tuyau de récupération (22), et des organismes inclus dans la solution de culture (W) peuvent être efficacement récupérés.
PCT/JP2024/008788 2023-03-14 2024-03-07 Appareil de culture et système de culture Pending WO2024190607A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2023-040143 2023-03-14
JP2023040143A JP2024130440A (ja) 2023-03-14 2023-03-14 培養装置および培養システム

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014176361A (ja) * 2013-03-15 2014-09-25 Kobelco Eco-Solutions Co Ltd 微細藻類の培養装置
WO2015087858A1 (fr) * 2013-12-09 2015-06-18 電源開発株式会社 Appareil dédié à la culture d'algues et système de culture d'algues
WO2020136208A1 (fr) * 2018-12-28 2020-07-02 Global Biotech, S.L. Système à base de microalgues pour la production de produits et son procédé d'utilisation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014176361A (ja) * 2013-03-15 2014-09-25 Kobelco Eco-Solutions Co Ltd 微細藻類の培養装置
WO2015087858A1 (fr) * 2013-12-09 2015-06-18 電源開発株式会社 Appareil dédié à la culture d'algues et système de culture d'algues
WO2020136208A1 (fr) * 2018-12-28 2020-07-02 Global Biotech, S.L. Système à base de microalgues pour la production de produits et son procédé d'utilisation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TAKAHITO HOSHINO , XIANGTING HUANG , HIROYUKI AOYAGI , SHINICHI AOKI , JUNPEI NOMURA , TAKUMI MATSUZAKI , MASAKI ENDO , JOS: "Possibility of industrial use of microalgae to achieve carbon neutrality: Introduction of Chitose Group's activities for industrial use of microalgae", JOURNAL OF ENVIRONMENTAL BIOTECHNOLOGY, vol. 22, no. 1, 1 January 2022 (2022-01-01), pages 47 - 51, XP009557349, DOI: 10.50963/jenvbio.22.1_47 *

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