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WO2024181072A1 - Fat mass composition, meat substitute, method for producing fat mass composition, and method for producing meat substitute - Google Patents

Fat mass composition, meat substitute, method for producing fat mass composition, and method for producing meat substitute Download PDF

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Publication number
WO2024181072A1
WO2024181072A1 PCT/JP2024/004147 JP2024004147W WO2024181072A1 WO 2024181072 A1 WO2024181072 A1 WO 2024181072A1 JP 2024004147 W JP2024004147 W JP 2024004147W WO 2024181072 A1 WO2024181072 A1 WO 2024181072A1
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WIPO (PCT)
Prior art keywords
meat
fat
composition
mass
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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PCT/JP2024/004147
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French (fr)
Japanese (ja)
Inventor
直樹 塚本
翔子 古岩井
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Fujifilm Corp
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Fujifilm Corp
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Publication of WO2024181072A1 publication Critical patent/WO2024181072A1/en
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Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23DEDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
    • A23D9/00Other edible oils or fats, e.g. shortenings or cooking oils
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23DEDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
    • A23D9/00Other edible oils or fats, e.g. shortenings or cooking oils
    • A23D9/007Other edible oils or fats, e.g. shortenings or cooking oils characterised by ingredients other than fatty acid triglycerides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/206Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
    • A23L29/244Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin from corms, tubers or roots, e.g. glucomannan
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/206Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
    • A23L29/256Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin from seaweeds, e.g. alginates, agar or carrageenan

Definitions

  • the present disclosure relates to a fat chunk composition, a meat substitute, a method for producing a fat chunk composition, and a method for producing a meat substitute.
  • Fat contained in meat has elasticity and a unique texture in which oil and fat oozes out when chewed.
  • the properties of fat are a factor that greatly affects the texture, flavor, etc., of meat when eaten.
  • alternative meat In recent years, from the viewpoint of maintaining health, attempts have been made to refrain from consuming livestock meat and to consume meat foods made from vegetable proteins derived from plants such as soybeans (hereinafter sometimes referred to as "alternative meat"). In order to make the texture and flavor of alternative meat closer to that of livestock meat, attempts have been made to include ingredients having a texture similar to that of fat in the alternative meat. Accordingly, development of ingredients having a texture similar to that of fat has been underway.
  • Japanese Patent No. 6265121 proposes "an oil-in-water emulsion soy protein gel food obtained by gelling an oil-in-water emulsion slurry containing 10 to 60% by weight of oil droplets having a particle size of 50 ⁇ m to 800 ⁇ m, wherein the planar occupancy rate of the oil droplets having a diameter of 50 ⁇ m to 800 ⁇ m is 10 to 60%, a gel made of a non-myosin gelling material exists as a continuous phase, and the gel is cross-linked by a protein cross-linking enzyme.”
  • JP 2014-87316 A describes a food product similar to unheated animal tissue having the following characteristics (A) to (D).
  • the food contains vegetable protein, fats and oils, and water, and the fats and oils are dispersed in an incompletely emulsified state;
  • the food contains a protein that has been intermolecularly cross-linked by the action of a protein cross-linking enzyme;
  • the food product is a gel-like tissue;
  • Japanese Patent Publication No. 1860/1974 proposes "a method for producing artificial adipose tissue, characterized in that an alkaline salt of alginic acid, an inhibitor, fat and an alkaline earth metal salt are mixed and reacted to form an alginate gel base tissue incorporating fat.”
  • an objective of an embodiment of the present disclosure is to provide a fat chunk composition having a texture similar to the fat contained in livestock meat, and a meat substitute comprising a fat chunk composition having a texture similar to the fat contained in livestock meat.
  • the present disclosure includes the following aspects.
  • Vegetable oil and fat A polyvalent metal salt of an ionically crosslinkable polymer; A fat mass composition comprising a gelled product of glucomannan.
  • ⁇ 4> A fat block composition according to any one of ⁇ 1> to ⁇ 3>, having a toughness of 1000 gw ⁇ cm/cm2 or more and 10000 gw ⁇ cm/ cm2 or less in a multiple bite test.
  • ⁇ 5> A red meat-like portion containing protein; A meat substitute comprising the fat chunk composition described in any one of ⁇ 1> to ⁇ 4>.
  • ⁇ 6> The alternative meat described in ⁇ 5>, which contains soy protein powder at the interface between the red meat-like portion and the fat block composition.
  • ⁇ 7> The alternative meat according to ⁇ 5> or ⁇ 6>, wherein the red meat-like portion is spongy or fibrous.
  • a fat chunk composition having a texture similar to the fat contained in livestock meat a method for producing the fat chunk composition, and a meat substitute containing a fat chunk composition having a texture similar to the fat contained in livestock meat and a method for producing the meat substitute.
  • the upper or lower limit of one numerical range may be replaced with the upper or lower limit of another numerical range.
  • the upper or lower limit of the numerical range may be replaced with a value shown in the examples.
  • Each component may contain multiple types of the corresponding substance.
  • the amount refers to the total amount of those multiple substances present in the composition, unless otherwise specified.
  • the term "process” includes not only independent processes, but also processes that cannot be clearly distinguished from other processes as long as the intended effect of the process is achieved. As used herein, a combination of two or more preferred aspects is a more preferred aspect.
  • the fat mass composition of this embodiment contains vegetable oil, a polyvalent metal salt of an ionically crosslinkable polymer, and a gel of glucomannan.
  • the fat chunk composition according to the present embodiment has a texture similar to that of fat contained in livestock meat due to the above-mentioned composition. The reason for this is presumed to be as follows.
  • the fat lump composition contains a vegetable oil and a polyvalent metal salt of an ionically crosslinkable polymer, so that a hardened material containing fatty oils and fats can be obtained in the fat lump composition.
  • the fat lump composition further contains a gel of glucomannan.
  • the glucomannan gel exhibits elasticity similar to that of fat contained in livestock meat.
  • the structure of the fat lump composition breaks down when chewed, causing the fatty oils and fats contained therein to seep out.
  • the fat block composition according to the present disclosure has elasticity and has the property of causing fats and oils to seep out when chewed.
  • the fat chunk composition of this embodiment has a texture similar to that of fat contained in livestock meat.
  • Vegetable oils and fats examples include rapeseed oil, soybean oil, palm oil, olive oil, rice bran oil, corn oil, and coconut oil. Vegetable oils and fats refer to those obtained using plants as raw materials.
  • the melting point of the vegetable oil is not particularly limited, but may be, for example, between -20°C and 300°C.
  • the melting point of the fat or oil is a value measured by a thermal analysis measuring device.
  • a thermal analysis measuring device for example, SSC5000DSC200 manufactured by Seiko Electronic Industries Co., Ltd. can be used.
  • the melting point of the oil or fat is measured by adding 3 mg of a sample to the apparatus and raising the temperature at a rate of 3° C./min.
  • the vegetable oil content is preferably 1% by mass or more and 50% by mass or less, more preferably 5% by mass or more and 40% by mass or less, and even more preferably 10% by mass or more and 35% by mass or less, based on the total fat mass composition.
  • the vegetable oil may be one type or a combination of two or more types.
  • ionically crosslinkable polymer By “ionically crosslinkable polymer” is meant a polymer that crosslinks upon reaction with ions. Preferably, the ionically cross-linkable polymer is an edible ionically cross-linkable polymer.
  • edible means the property of not having adverse effects on human health when orally ingested.
  • the ionically crosslinkable polymer may be a polysaccharide having at least one group selected from the group consisting of a carboxy group, a carboxylate anion group ( --COO.sup.- ), a sulfo group, and a sulfonate anion group ( --SO.sub.3.sup.- ).
  • Examples of the ionically crosslinkable polymer include alginic acid, carrageenan, LM pectin, HM pectin, gellan gum, etc., and at least one selected from the group consisting of alginic acid and LM pectin is preferred. It should be noted that "LM” is an abbreviation for "Low methoxyl” and "HM” is an abbreviation for "High methoxyl.”
  • the viscosity of a 1% by mass aqueous solution of the ionically crosslinkable polymer is 0.01 mPa ⁇ s or more and 3000 mPa ⁇ s or more.
  • the viscosity is preferably 0.1 mPa ⁇ s or less, more preferably 0.1 mPa ⁇ s or more and 2000 mPa ⁇ s or less, and further preferably 1 mPa ⁇ s or more and 1000 mPa ⁇ s or less.
  • the viscosity of a 1% by mass aqueous solution of the ionically crosslinkable polymer is measured using a viscometer.
  • the measurement conditions are, for example, as follows. ⁇ Conditions> Viscometer: TVB10 type (Toki Sangyo) Measurement temperature: 20°C
  • alginic acid is a type of polysaccharide, which is a dehydrated polymer of mannuronic acid and its isomer, glucuronic acid.
  • Polyvalent metal salts of alginic acid are salts in which ionic bonds are formed between the carboxylate anion ( -COO- ) of alginic acid and a polyvalent metal ion. Since the polyvalent metal ion forms ionic bonds with multiple carboxylate anions, the polyvalent metal salts of alginic acid have a structure in which alginic acid is crosslinked via the polyvalent metal ions.
  • the polyvalent metal ion is preferably a metal ion having an ionic valence of divalent or higher.
  • polyvalent metal ions include divalent metal ions such as calcium ion, magnesium ion, iron (II), copper (II), zinc ion, and manganese ion; and trivalent metal ions such as aluminum ion and iron (III) ion.
  • the polyvalent metal ion is at least one selected from calcium ions, magnesium ions, and zinc ions, and it is more preferable that the polyvalent metal ion is calcium ions.
  • polyvalent metal salts of ionically cross-linkable polymers include calcium alginate, calcium carrageenan, LM pectin calcium, HM pectin calcium, gellan gum calcium, etc., with calcium alginate and LM pectin calcium being preferred.
  • the content of the ionically cross-linkable polymer is preferably 0.001% by mass or more and 5% by mass or less, more preferably 0.01% by mass or more and 4% by mass or less, and even more preferably 0.1% by mass or more and 3% by mass or less, based on the total fat mass composition.
  • Polyvalent metal ions can be obtained, for example, by stirring an aqueous solution containing a monovalent metal salt of alginic acid, such as sodium alginate, in an aqueous solution containing polyvalent metal ions.
  • a monovalent metal salt of alginic acid such as sodium alginate
  • Glucomannan is a type of konjac (Amonphophallus konjac), which is a type of konjac that is grown in the Araceae family. It is a polysaccharide isolated from the rhizome of the plant, Cannabis sativa (Konnjac), and is composed of glucose and mannose. Specifically, glucomannan is a polysaccharide in which D-glucose and D-mannose are bonded via ⁇ -1,4 bonds in a ratio of 2:3.
  • Glucomannan is dissolved in water and gelled by adding alkali.
  • the fat mass composition according to the present disclosure comprises a glucomannan gel obtained as described above.
  • the glucomannan gel refers to one that contains at least glucomannan and water and behaves as an elastic solid.
  • the glucomannan content is preferably 0.01% by mass or more and 7% by mass or less, more preferably 0.1% by mass or more and 6% by mass or less, and even more preferably 1% by mass or more and 5% by mass or less, based on the total fat mass composition.
  • the content of the glucomannan gel is preferably 10% by mass or more and 90% by mass or less, more preferably 15% by mass or more and 80% by mass or less, and even more preferably 25% by mass or more and 75% by mass or less, based on the total fat mass composition.
  • the fat mass composition may contain additives other than the vegetable oil, the polyvalent metal salt of an ionically crosslinkable polymer, and the glucomannan gelatinized product.
  • additives include seasonings, acidulants, bittering agents, spices, sweeteners, antioxidants, colorants, color formers, fragrances, stabilizers, preservatives, etc.
  • the content of the other additives is preferably from 0 to 10% by mass.
  • the fat mass composition preferably comprises vegetable oil covered with a gel of polyvalent metal alginate and glucomannan.
  • vegetable oil is covered with a gel of polyvalent metal alginate and glucomannan
  • the phrase "vegetable oil is covered with a gel of polyvalent metal alginate and glucomannan” means that the gel of polyvalent metal alginate and glucomannan contains vegetable oil inside to the extent that the vegetable oil does not seep out.
  • Fat contained in livestock meat contains fat cells containing fats and oils inside.
  • the vegetable oil is dispersed in large numbers in the fat mass composition in a nearly spherical form (hereinafter referred to as "oil droplets").
  • the fat contained in livestock meat is composed of numerous fat cells. Therefore, the fat contained in livestock meat contains numerous oil droplets.
  • the structure of the fat chunk composition becomes similar to the structure of the fat contained in livestock meat, and the fat chunk composition is more likely to have a texture similar to that of the fat contained in livestock meat.
  • the hardness of the fat block composition is evaluated by its toughness in a multiple bite test.
  • the toughness in the multiple bite test is preferably from 1000 (gw ⁇ cm/ cm2 ) to 10,000 (gw ⁇ cm/ cm2 ), more preferably from 1500 (gw ⁇ cm/ cm2 ) to 8,000 (gw ⁇ cm/ cm2 ), and even more preferably from 2000 (gw ⁇ cm/ cm2 ) to 6,000 (gw ⁇ cm/ cm2 ).
  • the toughness in the multiple bite test is a value measured by a viscoelasticity tester.
  • a viscoelasticity tester for example, a Tensipressor MyBpy2 system manufactured by Takemoto Denki Co., Ltd. can be used.
  • the method for measuring toughness in the multiple bite test will now be described in detail.
  • the toughness is measured by the multiple bite test. The sample is cut into a size of 30 mm square and 5 mm thick. The sample is set on the stage of the viscoelasticity tester and measured three times under the measurement conditions of the multiple bite test. The average value is taken as the measured value.
  • the substitute meat preferably has a lean meat-like portion containing protein and a fat mass composition.
  • the fat chunk composition the fat chunk composition already described is applied.
  • the lean meat-like portion refers to the portion of the alternative meat that corresponds to the portion that looks like lean meat.
  • the lean meat-like portion preferably contains protein, and, if necessary, contains fats and oils, binders, and other additives.
  • the lean portion contains protein.
  • the protein preferably contains at least one of a vegetable protein and an animal protein, and more preferably contains a vegetable protein.
  • Vegetable protein is protein obtained from plants.
  • the vegetable protein is not particularly limited as long as it is a protein extracted from a plant.
  • sources of vegetable proteins include grains such as wheat, barley, oats, rice, and corn; beans such as soybeans, peas, red beans, chickpeas, lentils, fava beans, mung beans, and lupins; nuts and seeds such as almonds, peanuts, cashew nuts, pistachios, hazelnuts, macadamia nuts, flaxseed, sesame, rapeseed, cottonseed, safflower, and sunflowers; tubers such as potato, sweet potato, mountain yam, Jerusalem artichoke, and cassava; vegetables such as asparagus, artichoke, cauliflower, broccoli, and edamame; fruits such as bananas, jackfruit, kiwi fruit, coconut, avocado, and olives; mushrooms such as mushrooms, king oyster mushrooms, shiitake mushrooms, shimeji mushrooms,
  • the origin of the edible protein is preferably at least one selected from the group consisting of wheat, soybeans, peas, and rice, and more preferably at least one selected from the group consisting of soybeans and wheat.
  • the vegetable protein may contain a protein derived from one type of plant, or may contain proteins derived from two or more types of plants.
  • Animal protein is a protein obtained from an animal.
  • the animal protein may be obtained from an animal, or a protein having the same amino acid sequence as the protein obtained from an animal may be produced by cell culture or enzyme reaction and extracted.
  • the animal protein is not particularly limited as long as it is a protein obtained from an animal, and examples of the animal protein include collagen, gelatin, keratin, fibroin, sericin, casein, conchiolin, elastin, protamine, egg yolk protein, and egg white protein.
  • the animal protein may contain only one type, or may contain two or more types.
  • the protein has a muscle-like tissue.
  • muscle-like tissue refers to tissue that has a structure similar to a bundle of fibers and can be split into fibers in a certain direction.
  • Red meat of livestock originates from muscle.
  • muscle is composed of muscle fiber bundles. Therefore, red meat of livestock has a structure like a fiber bundle.
  • a method for making a protein have a muscle-like structure a method of extruding a protein (water or the like may be added together with the protein, if necessary) using an extruder can be mentioned.
  • the protein By extruding a protein while applying a shear stress in the extrusion direction, the protein has a structure similar to a bundle of fibers aligned in the extrusion direction of the extruder, and has a texture that can be split into fibers in the extrusion direction of the extruder.
  • the protein content of the entire red meat-like portion is preferably 50% to 100% by mass, more preferably 60% to 95% by mass, and even more preferably 70% to 90% by mass.
  • the lean meat-like portion may contain fats and oils.
  • the oil or fat may be contained in a liquid state, or may be contained in a solid state by a method such as gelation or emulsification.
  • the oils and fats include vegetable oils and fats, animal oils and fats, and the like.
  • examples of the vegetable oil include the same ones as those mentioned in the description of the fat mass composition.
  • animal fats and oils include beef tallow, lard, whale fat, and fish oil.
  • the fat content in the red meat-like portion is preferably 0% by mass or more and 50% by mass or less, more preferably 0% by mass or more and 40% by mass or less, and even more preferably 0% by mass or more and 30% by mass or less, based on the entire red meat-like portion.
  • the lean meat-like portion preferably contains a binder as necessary.
  • the red meat-like portion can easily maintain a unified shape.
  • the binder is not particularly limited as long as it is edible and can maintain the shape of the red meat-like portion.
  • examples of the binder include proteins, thickening polysaccharides, starch, enzymes that harden proteins, etc.
  • the binders may be used alone or in combination.
  • the protein used as the binder may be the same as or different from the protein contained in the lean meat-like portion.
  • proteins used as binders include vegetable proteins and animal proteins.
  • examples of vegetable proteins used as binders include proteins derived from wheat, soybeans, rice, and the like.
  • examples of animal proteins used as binders include milk proteins and egg whites.
  • enzymes that harden proteins include transglutaminase, etc.
  • Commercially available transglutaminase products can be used, such as the Activa (registered trademark) series manufactured by Ajinomoto Co., Inc.
  • Thickening polysaccharides include, for example, carrageenan, xanthan gum, pectin, locust bean gum, curdlan, guar gum, tragacanth gum, gum arabic, gellan gum, tamarind seed gum, cassia gum, tara gum, alginic acid, agar, glucomannan, soybean polysaccharides, gelatin, pullulan, psyllium, chitosan, methylcellulose, hydroxypropylmethylcellulose, hydroxyethylcellulose, carboxymethylcellulose, dextrin, etc.
  • starches examples include wheat starch, cassava starch, rice starch, glutinous rice starch, corn starch, waxy corn starch, sago starch, potato starch, kudzu starch, lotus root starch, mung bean starch, sweet potato starch, waxy potato starch, waxy cassava starch, waxy wheat starch, etc.
  • the content of the binder contained in the red meat-like portion is preferably 0.01% by mass or more and 30% by mass or less, more preferably 0.1% by mass or more and 25% by mass or less, and even more preferably 1% by mass or more and 20% by mass or less, based on the entire red meat-like portion.
  • the lean meat-like portion preferably contains other additives other than the protein, fats and oils, and binders, as necessary.
  • other additives include water, seasonings, acidulants, bittering agents, spices, sweeteners, antioxidants, colorants, color formers, fragrances, stabilizers, preservatives, etc.
  • the content of the other additives is preferably from 0 to 20% by mass.
  • the lean portion is preferably spongy or fibrous. By making the lean meat-like portion spongy or fibrous, it becomes easier to produce a raw meat-like substitute that has a texture similar to that of a chunk of meat.
  • spongy refers to a porous structure that appears isotropic
  • fibrous refers to a fibrous structure that appears anisotropic
  • anisotropic porous structure refers to a structure in which the pore shape of any cross section is approximately elliptical and is approximately the same regardless of the direction.
  • the anisotropic fiber structure refers to a structure in which the pore shape in the cut section varies depending on the direction, being generally elliptical or generally fibrous.
  • the protein contained in the lean meat-like portion is preferably produced by adding raw material protein and water to an extruder and kneading and extruding.
  • the temperature conditions for kneading and extruding are preferably above the boiling point of water, so that when the mixture is extruded to atmospheric pressure immediately after kneading, the protein swells due to the boiling of water, has a porous structure, and the lean meat-like portion becomes spongy.
  • shear stress is applied immediately after kneading, the proteins tend to be oriented in a fibrous form, and the red meat-like portion becomes fibrous.
  • An example of a method for controlling the lean meat-like portion to be fibrous is to control the protein contained in the lean meat-like portion by using or not using a die when extruding the protein from an extruder. For example, when a die is used to extrude the protein contained in the lean meat portion from an extruder, the lean meat portion tends to become fibrous. A specific method for producing the lean meat-like portion will be described later.
  • the lean meat-like portion is spongy or fibrous, resulting in a raw meat substitute that has a texture closer to that of a whole piece of meat. Furthermore, from the viewpoint of producing a raw meat-like meat substitute having a texture similar to that of whole meat, it is further preferable that the lean meat-like portion is fibrous.
  • soy protein powder at the interface between the lean meat-like portion and the fat chunk composition. This composition improves adhesion between the lean meat-like portion and the fat chunk composition, resulting in a meat substitute that can maintain an appearance more similar to that of livestock meat.
  • Soy protein powder refers to a powder containing soy protein as the main component.
  • the term "main component” refers to a soy protein powder containing the relevant component in an amount of 90% by mass or more based on the entire powder.
  • soy protein powder examples include Fujipro FR (manufactured by Fuji Oil Co., Ltd.), Showa Fresh RF (manufactured by Showa Sangyo Co., Ltd.), and Soya Flour FT-N (manufactured by Nisshin Oillio Co., Ltd.).
  • soy protein powder is contained at the interface between the red meat-like portion and the fat chunk composition is determined by observing the interface between the red meat-like portion and the fat chunk composition under a microscope. Specifically, the procedure is as follows. The sample is frozen, cut at an angle of 10° to the interface between the lean meat portion and the fat chunk composition, and the interface portion of the cross section is observed under an optical microscope. It is observed that the components of the fat chunk composition and the soy protein powder are mixed.
  • the method for producing a fat chunk composition preferably includes a step of emulsifying vegetable oil and an aqueous solution containing an ionically cross-linkable polymer to obtain an emulsion (emulsification step), a step of mixing the emulsion with a solution containing glucomannan and calcium hydroxide to obtain a mixed liquid (mixing step), and a step of heating the mixed liquid (heating step).
  • the emulsification step is a step of emulsifying a vegetable oil and an aqueous solution containing an ionically crosslinkable polymer to obtain an emulsion.
  • the vegetable oils and fats used in the emulsification step include those already mentioned.
  • the ionically crosslinkable polymer may be the same as those mentioned above.
  • the solution containing the ionically crosslinkable polymer is preferably a solution containing a monovalent metal salt of the ionically crosslinkable polymer.
  • the monovalent metal salt of an ionically crosslinkable polymer is a salt in which at least one of a carboxylate anion ( --COO.sup.- ) and a sulfonic acid anion group ( --SO.sub.3.sup.- ) contained in the ionically crosslinkable polymer forms an ionic bond with a monovalent metal ion.
  • an alkali metal ion is preferred, a lithium ion, a sodium ion and a potassium ion are more preferred, and a sodium ion is most preferred.
  • a specific example of the monovalent metal salt of an ionically crosslinkable polymer is sodium alginate.
  • the content of the ionically cross-linkable polymer in the aqueous solution is preferably 0.1% by mass or more and 10% by mass or less based on the total amount of the aqueous solution.
  • the mass ratio of the vegetable oil to the aqueous solution containing the ionically crosslinkable polymer is preferably 1/5 or more and 5/1 or less.
  • the method for emulsifying the vegetable oil and the aqueous solution containing the ionically cross-linkable polymer is not particularly limited, and a method of emulsifying a solution containing the vegetable oil and the aqueous solution containing the ionically cross-linkable polymer using an emulsifier is preferred.
  • emulsifiers examples include rotary mixers equipped with propeller-type, anchor-type, paddle-type, or turbine-type stirring blades, static mixers such as static mixers, rotor-stator type emulsifiers such as homogenizers and Clearmix, mill-type emulsifiers equipped with a grinding function, high-pressure emulsifiers such as Manton-Gaulin pressure emulsifiers, high-pressure nozzle-type emulsifiers that generate cavitation under high pressure, high-pressure collision-type emulsifiers such as microfluidizers that apply shear force by colliding liquids together under high pressure, ultrasonic emulsifiers that generate cavitation using ultrasound, and membrane emulsifiers that perform uniform emulsification through fine pores.
  • high-pressure emulsifiers such as Manton-Gaulin pressure emulsifiers, high-pressure nozzle-type emulsifiers that generate cavitation under high pressure,
  • an edible surfactant When emulsifying the vegetable oil and the aqueous solution containing the ionically crosslinkable polymer, an edible surfactant may be added.
  • edible surfactants include glycerin fatty acid esters, polyglycerin fatty acid esters, organic acid monoglycerides, sorbitan fatty acid esters, propylene glycol fatty acid esters, sucrose fatty acid esters, polyglycerin condensed ricinoleic acid esters, and lecithin.
  • the surfactant it is preferable to add the surfactant so that the content of the surfactant in the entire emulsion is 0.1% by mass or more and 5% by mass or less.
  • the emulsion is preferably an oil-in-water emulsion, since it is easy to form a structure in which the vegetable oil is covered with a gel of polyvalent metal alginate and glucomannan.
  • the mixing step is a step of mixing the emulsion obtained through the emulsification step with a solution containing glucomannan and calcium hydroxide to obtain a mixed liquid.
  • the emulsion obtained through the emulsification process contains an ionically cross-linkable polymer. This compound undergoes an ion exchange reaction in the presence of polyvalent metal ions, producing a cross-linked polymer structure. It is assumed that this reaction mainly takes place during the mixing process.
  • the content of glucomannan in the solution containing glucomannan and calcium hydroxide is preferably 0.1% by mass or more and 10% by mass or less, more preferably 0.2% by mass or more and 8% by mass or less, and even more preferably 0.5% by mass or more and 5% by mass or less, based on the entire solution containing glucomannan and calcium hydroxide.
  • the content of calcium hydroxide in the solution containing glucomannan and calcium hydroxide is preferably 0.01% by mass or more and 1% by mass or less, more preferably 0.02% by mass or more and 0.8% by mass or less, and even more preferably 0.03% by mass or more and 0.5% by mass or less, based on the entire solution containing glucomannan and calcium hydroxide.
  • the mass ratio of the emulsion obtained through the emulsification process to the solution containing glucomannan and calcium hydroxide added when preparing the mixed liquid is preferably 1/2 or more and 2/1 or less.
  • stirrer As a method for mixing the emulsion obtained through the emulsification step with the solution containing glucomannan and calcium hydroxide, a method in which these solutions are mixed with a stirrer can be mentioned.
  • the stirrer include rotary stirrers equipped with propeller-type, anchor-type, paddle-type, or turbine-type stirring blades.
  • the heating step is a step of heating the mixed liquid obtained through the mixing step.
  • the mixture obtained through the mixing step contains glucomannan.
  • the compound gels in the presence of an alkali to produce a gel of glucomannan. It is presumed that this reaction mainly proceeds during the heating step.
  • the mixed liquid obtained through the mixing step is preferably heated to 80° C. or higher and 120° C. or lower.
  • the heating time is preferably from 5 minutes to 30 minutes.
  • the heating process involves adding the mixture obtained through the mixing process to boiling water and heating it.
  • the fat block composition obtained through the above steps tends to have a structure in which the vegetable oil is covered with a polyvalent metal salt of an ionically crosslinkable polymer and a gelled product of glucomannan.
  • the method for producing the alternative meat preferably includes a step (first step) of contacting the red meat-like portion with the fat chunk composition, and a step (second step) of fixing the mixture using a binder.
  • the method for producing alternative meat according to the present disclosure is preferably carried out as follows.
  • Step (1-1) A lean meat-like raw material containing at least protein is extruded from an extruder, and the extruded lean meat-like raw material is colored red and then molded into a shape similar to that of lean meat of livestock.
  • Step (1-2) Color commercially available soy meat with a red coloring agent, and mold the colored substitute meat into a shape similar to that of red meat from livestock.
  • Step (1-3) A raw material of a red meat-like portion containing at least a protein and a colorant is extruded from an extruder, and the extruded red meat-like portion colored red is formed into a shape resembling the shape of a block of red meat.
  • the lean meat-like portion raw material contains at least protein, but from the viewpoint of efficient extrusion of the lean meat-like portion raw material from the extruder, it is preferable that the lean meat-like portion raw material also contains water.
  • the lean meat-like portion of the raw material preferably contains 2 to 6 parts by mass of water per 10 parts by mass of protein.
  • the extruder is not particularly limited, and a known single screw extruder, non-intermeshing counter-rotating twin screw extruder, intermeshing counter-rotating twin screw extruder, or intermeshing co-rotating twin screw extruder can be used.
  • the extruder barrel temperature is preferably 60°C or higher and 100°C or lower in the front half of the barrel (from the raw material supply section for the lean part to the center of the barrel), 90°C or higher and 170°C or lower in the center of the barrel (center of the axial length of the barrel), and 140°C or higher and 180°C or lower in the rear half of the barrel (from the center of the barrel to the tip of the barrel).
  • the extruder preferably has a die attached to the end of the barrel.
  • the die is preferably one which results in a sheet-like extrudate.
  • the gap (lip clearance) of the discharge port of the die is preferably 1 mm or more and 10 mm or less.
  • the shape of the discharge port may be flat or cylindrical.
  • the length of the die is preferably 30 mm or more and 500 mm or less.
  • the die is preferably a cooling die, which means a die that is cooled, for example, by circulating a cooling liquid (such as water or glycol). The cooling may be performed by circulating cooling water or by air cooling.
  • the use of a cooling die tends to suppress the expansion of the extruded red meat-like portion raw material, and therefore the red meat-like portion produced using the red meat-like portion raw material extruded using the cooling die tends to be fibrous.
  • the temperature of the discharge port of the cooling die is preferably set to 90° C. or higher and 120° C. or lower.
  • the extruded lean meat-like portion of the raw material is preferably cut into pieces for use as required.
  • the length of the extruded red meat-like portion in the extrusion direction of the raw material is 10 mm or more and 500 mm or less, and the length in the direction perpendicular to the extrusion direction is 2 mm or more and 8 mm or less.
  • the extruded lean meat raw material is preferably coloured red with a colouring agent.
  • the coloring agent is preferably an edible red coloring agent.
  • coloring agents include natural beetroot red pigment, cochineal pigment, gardenia red pigment, etc., and among these, natural beetroot red pigment is preferred because it fades due to the heat during cooking.
  • the extruded raw material of the lean meat-like portion is collected in a block form and molded into a shape similar to that of lean livestock meat, thereby producing the lean meat-like portion of the substitute meat. From the viewpoint of obtaining a substitute meat having an appearance closer to that of livestock meat, when the extruded lean meat-like portions of the raw material are collected in a block, it is preferable to align the extrusion directions of the extruded lean meat-like portions of the raw material in approximately the same direction.
  • the procedure for producing the lean meat-like portion may involve coloring commercially available soy meat with a red coloring agent and molding the colored substitute meat into a shape that resembles the shape of lean livestock meat.
  • Soy meat is a food ingredient artificially produced using ingredients containing vegetable protein derived from soybeans, and has a texture similar to that of animal meat.
  • soy meat it is preferable to cut the soybean meat into pieces before use, as needed. From the viewpoint of obtaining a substitute meat having an appearance similar to that of livestock meat, it is preferable to adjust the size of soy meat by splitting it along a structure similar to the fiber bundles of muscle-like tissues of soy meat, in the case of general commercially available fiberized soy meat. For example, it is preferable to set the length of the soy meat to 10 mm to 50 mm, the width to 2 mm to 8 mm, and the thickness to 1 mm to 5 mm.
  • the soy meat is preferably coloured red with a colouring agent.
  • the coloring agent may be the same as those listed in step (1-1). It is also preferable to add a binder to the soy meat, and seasonings may be added as necessary.
  • the meat is collected into a lump and molded into a shape similar to that of red meat from livestock, thereby producing the red meat-like portion of the substitute meat.
  • Procedure (1-3) It is preferable to produce the red meat-like portion in the same manner as in procedure (1-1), except that instead of adding the colorant to the raw material of the red meat-like portion after extrusion molding, the colorant is added to the raw material of the red meat-like portion before extrusion molding.
  • Procedure (1-4) It is preferable to produce a red meat-like portion in the same manner as in procedure (1-2), except that instead of coloring commercially available soy meat with a coloring agent, soy meat that has already been colored red is used.
  • the first step is to contact the lean meat-like portion with a fat chunk composition.
  • the method of contacting the lean meat-like portion with the fat chunk composition is not particularly limited, but for example, when producing a substitute meat having an appearance similar to steak meat as shown in Figure 1, it is preferable to mold the lean meat-like portion into a shape similar to the lean meat of steak meat, mold the fat chunk composition into a shape similar to the fat of steak meat, and then contact the lean meat-like portion with the fat chunk composition.
  • the first step is preferably a step of attaching powdered soy protein to a portion of the fat lump composition, and adhering a red meat-like portion containing protein to the area of the fat lump composition where the powdered soy protein has been attached.
  • the lean meat-like portion is molded into a shape similar to the lean meat of steak meat, and the fat chunk composition is molded into a shape similar to the fat of steak meat.
  • powdered soy protein is attached to the area of the fat chunk composition that will come into contact with the lean meat-like portion. This is followed by a process in which the protein-containing lean meat-like portion is adhered to the area where the powdered soy protein has been attached.
  • the meat substitute produced using this process tends to contain soy protein powder at the interface between the red meat-like portion and the fat chunk composition. This makes it easier to obtain meat substitutes with improved adhesion between the red meat-like portion and the fat chunk composition.
  • the second step is a step of fixing the contact body of the lean meat-like portion obtained in the first step and the fat chunk composition.
  • a method for fixing the contact body for example, there is a method in which the contact body is left stationary while a certain pressure is applied thereto.
  • the contact body may be entirely coated with a binder and then allowed to stand.
  • the binder those already mentioned can be used, but it is preferable that the binder contains an enzyme that hardens proteins, and it is more preferable that the binder contains transglutaminase.
  • Example 1 A fat mass composition was obtained by the following procedure. (Emulsification process) 300 parts by mass of an aqueous solution containing an ionically crosslinkable polymer, in which the content of sodium alginate (Kimica Algin I-1 manufactured by Kimica) as the ionically crosslinkable polymer was 1% by mass relative to the entire solution, and 200 parts by mass of coconut oil (imported and sold by Alcapia, product name: Pia Cocona) as the vegetable oil were stirred for 1 minute at 600 rpm (revolutions per minute) using a rotary mixer equipped with a propeller-type stirring blade, to obtain emulsion 1.
  • the viscosity (20° C.) of a 1% by mass aqueous solution of sodium alginate was 71.1 mPa ⁇ s.
  • glucomannan As glucomannan, 50 parts by mass of konjac flour (glucomannan) manufactured by Asahi Foods Industries Co., Ltd. was dissolved in 1620 parts by mass of water and left for 30 minutes. After that, a dispersion of 3 parts by mass of calcium hydroxide in 180 parts by mass of water was added to this solution and thoroughly stirred to obtain a gel containing glucomannan and calcium hydroxide (gelated glucomannan). To the emulsion 1, 500 parts by mass of a gel containing glucomannan and calcium hydroxide was added, and the mixture was stirred with a stirrer to obtain a mixed gel 1.
  • the mixed gel 1 contains, in addition to the gel of coconut oil and glucomannan, a calcium salt of sodium alginate (a polyvalent metal salt of an ionically crosslinkable polymer).
  • Fat block composition 1 had a structure in which vegetable oil was covered with a gel of polyvalent metal alginate and glucomannan.
  • Fat block composition 2 was obtained in the same manner as in Example 1, except that LM pectin (manufactured by Unicook Foods, product name Milky Ribbon) was used instead of sodium alginate as the ionically cross-linkable polymer.
  • Fat block composition 2 had a structure in which vegetable oil was covered with a polyvalent metal salt of LM pectin and a gelled product of glucomannan.
  • the viscosity (20° C.) of a 1% by mass aqueous solution of LM pectin was 3.13 mPa ⁇ s.
  • Example 3 Fat block composition 3 was obtained in the same manner as in Example 1, except that 250 parts by mass of a gel containing glucomannan and calcium hydroxide was added to 750 parts by mass of emulsion 1 and stirred with a stirrer to form a mixed gel. Fat block composition 3 had a structure in which vegetable oil was covered with a gel of polyvalent metal alginate and glucomannan.
  • Example 4 Fat block composition 4 was obtained in the same manner as in Example 1, except that 333 parts by mass of a gel containing glucomannan and calcium hydroxide was added to 667 parts by mass of emulsion 1 and stirred with a stirrer to form a mixed gel. Fat block composition 4 had a structure in which vegetable oil was covered with a gel of polyvalent metal alginate and glucomannan.
  • Fat block composition 5 was obtained in the same manner as in Example 1, except that 667 parts by mass of a gel containing glucomannan and calcium hydroxide was added to 333 parts by mass of emulsion 1, and stirred with a stirrer to form a mixed gel. Fat block composition 5 had a structure in which vegetable oil was covered with a gel of polyvalent metal alginate and glucomannan.
  • Fat block composition 6 was obtained in the same manner as in Example 1, except that 750 parts by mass of a gel containing glucomannan and calcium hydroxide was added to 250 parts by mass of emulsion 1 and stirred with a stirrer to form a mixed gel. Fat block composition 6 had a structure in which vegetable oil was covered with a gel of polyvalent metal alginate and glucomannan.
  • ⁇ Comparative Example 1 75 parts by mass of a protein suspension having a defatted soybean (Fujipro FR manufactured by Fuji Oil Co., Ltd.) content of 15% relative to the total solution, and 25 parts by mass of coconut oil (imported and sold by Alcapia Co., Ltd., product name: Pia Cocona) as a vegetable oil were stirred at 600 rpm for 1 minute using a rotary mixer equipped with a propeller-type stirring blade, to obtain emulsion 2.
  • a protein suspension having a defatted soybean (Fujipro FR manufactured by Fuji Oil Co., Ltd.) content of 15% relative to the total solution and 25 parts by mass of coconut oil (imported and sold by Alcapia Co., Ltd., product name: Pia Cocona) as a vegetable oil were stirred at 600 rpm for 1 minute using a rotary mixer equipped with a propeller-type stirring blade, to obtain emulsion 2.
  • a crosslinker liquid (10% by weight aqueous solution of Supercard manufactured by Ajinomoto Co., Ltd.) was added to the emulsion 2, and the mixture was hardened at 55°C for 30 minutes, followed by an enzyme inactivation treatment at 90°C for 30 minutes to obtain fat block composition C1.
  • a cooling die (lip clearance: 3 mm) was attached, and the outlet temperature of the cooling die was stabilized at 105° C.
  • Mixed powder 1 was introduced into the extruder at 250 g/min, and discharged from the extruder while adding water in an amount of 50% by mass of the mixed powder 1 to the extruder, to obtain Raw material 1 of a lean meat-like portion having muscle-like tissue in the extrusion direction (fibrous).
  • Example 1 (Preparation of fat chunk composition) The mixed gel 1 obtained in Example 1 was molded, one side of it was sprinkled with defatted soybean flour (Fujipro FR manufactured by Fuji Oil Co., Ltd.; powdered soybean protein) and the surface was lightly kneaded, and the mixture was left to stand for 1 hour, then placed in boiling water for 5 minutes and cooled to obtain fat block composition 101.
  • defatted soybean flour Frazier FR manufactured by Fuji Oil Co., Ltd.; powdered soybean protein
  • the fat chunk composition 1 cut to a 5 mm square cross section was mixed with a part of the lean meat-like portion precursor A, and the lean meat-like portion precursor A mixed with the fat chunk composition 1 was arranged in one direction and stacked, while the fat chunk composition 101 cut to an appropriate size was placed on top of the lean meat-like portion precursor A so that the surface coated with defatted soybean flour faces the textured soybean protein of the lean meat-like portion precursor A. At this time, the defatted soybean flour is present at the interface between the lean meat-like portion and the fat chunk composition 101. Next, the whole was shaped to resemble a block of sirloin steak, and pressure was applied for 2 hours to form the block of meat-like food 1. The block of meat-like food 1 was cut to a thickness of 25 mm perpendicular to the fiber direction to obtain a steak-like marbled raw meat substitute 1 (substitute meat). A photograph of the obtained meat substitute is shown in Figure 1.
  • Example 102 Except for using commercially available fibrous soy meat (Vegetarian Butcher, What the Cluc) instead of the lean meat-like raw material 1, the lean meat-like precursor and the substitute meat were prepared in the same manner as in Example 101, and a steak-shaped marbled raw meat-like substitute meat 2 was obtained.
  • fibrous soy meat Veetarian Butcher, What the Cluc
  • Example 103 The same procedures as in Example 101 were used to prepare a lean meat precursor and a substitute meat, except that instead of the lean meat-like raw material 1, commercially available spongy soy meat (Apex-1000 manufactured by Fuji Oil Co., Ltd.) that had been rehydrated in hot water for 10 minutes was used, and a steak-like marbled raw meat-like substitute meat 3 was obtained.
  • spongy soy meat Apex-1000 manufactured by Fuji Oil Co., Ltd.
  • Example 101> Except for not performing (preparation of fat block composition) in ⁇ Example 101> and changing the procedure for (preparation of substitute meat) as follows, the same procedure as in Example 101 was followed to prepare a lean meat-like portion precursor and a substitute meat, thereby obtaining a steak-like marbled raw meat-like substitute meat C1.
  • the fat chunk composition C1 cut into a 5 mm square strip was mixed with a part of the lean meat-like portion precursor A, and the lean meat-like portion precursor A mixed with the fat chunk composition C1 was arranged in one direction and stacked. The whole was then shaped to resemble a block of sirloin steak, and pressure was applied for 2 hours to form the block of meat-like food C1.
  • the block of meat-like food 1 was cut into a thickness of 25 mm perpendicular to the fiber direction to obtain a steak-like marbled raw meat substitute C1 (substitute meat).
  • the fat block composition and steak-shaped marbled raw meat substitute obtained in each example were cooked on a hot plate.
  • the fat block composition and steak-shaped marbled raw meat substitute after cooking were evaluated as follows.
  • the contents of the table will be explained below.
  • the "polyvalent metal ion species” refers to the polyvalent metal ion contained in the polyvalent metal salt of the ionically crosslinkable polymer.
  • "Beef fat” indicates that beef fat was used. Note that only the multiple bite test was performed.
  • "Pork fat” indicates that pork fat was used. Note that only the multiple bite test was performed.
  • "Presence or absence of powdery body at the interface” indicates whether or not there is a soy protein fraction at the interface between the red meat-like portion and the fat chunk composition. If there is a powdery body, it is recorded as “present”, and if there is no powdery body, it is recorded as "-”.
  • the fat chunk composition and meat substitute of this example have a texture similar to the fat contained in livestock meat, and contain a fat chunk composition that has a texture similar to the fat contained in livestock meat.

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Abstract

Provided are: a fat mass composition containing a plant-based oil and fat, a polyvalent metal salt of an ion-exchanged polymer, and a gelled article of glucomannan; a meat substitute; a method for producing a fat mass composition; and a method for producing a meat substitute.

Description

脂肪塊組成物、代替肉、脂肪塊組成物の製造方法、及び代替肉の製造方法Fat chunk composition, meat substitute, method for producing fat chunk composition, and method for producing meat substitute

 本開示は、脂肪塊組成物、代替肉、脂肪塊組成物の製造方法、及び代替肉の製造方法に関する。 The present disclosure relates to a fat chunk composition, a meat substitute, a method for producing a fat chunk composition, and a method for producing a meat substitute.

 畜肉に含まれる脂身は、弾力を有し、咀嚼時に油脂が滲みだす独特の食感を有するものである。そして、脂身の性状は畜肉を食した際における食感、風味などに大きく影響を与える要素である。
 近年、健康維持の観点から、畜肉の摂取を控え、大豆等の植物由来の植物性タンパク質を原料とする肉用食品(以下、「代替肉」と称することがある)を摂取する試みがなされている。そして、代替肉の食感、風味などをより畜肉に近づけるために、脂身に似た食感を有する食材を代替肉中に含ませる試みがなされている。そしてそれに伴い、脂身に似た食感を有する食材の開発が行われている。
Fat contained in meat has elasticity and a unique texture in which oil and fat oozes out when chewed. The properties of fat are a factor that greatly affects the texture, flavor, etc., of meat when eaten.
In recent years, from the viewpoint of maintaining health, attempts have been made to refrain from consuming livestock meat and to consume meat foods made from vegetable proteins derived from plants such as soybeans (hereinafter sometimes referred to as "alternative meat"). In order to make the texture and flavor of alternative meat closer to that of livestock meat, attempts have been made to include ingredients having a texture similar to that of fat in the alternative meat. Accordingly, development of ingredients having a texture similar to that of fat has been underway.

 例えば、特許第6265121号公報には「50μm~800μmの粒子径を有する油滴を10~60重量%含有する水中油型乳化スラリーが、ゲル化処理されて得られる、水中油型乳化物大豆蛋白質ゲル食品であって、50μm~800μmの直径を有する油滴の平面占有率が10~60%であり、非ミオシンのゲル化素材によるゲルが連続相として存在し、ゲルが蛋白質架橋酵素により架橋されたものである、水中油型乳化物大豆蛋白質ゲル食品。」が提案されている。
 特開2014-87316号公報には「下記(A)~(D)の特徴を有する未加熱動物組織様の食品。
 (A)該食品中に植物性タンパク質、油脂及び水を含み、油脂が不完全乳化状態で分散していること、
 (B)該食品がタンパク質架橋酵素の作用により分子間架橋されたタンパク質を含有するものであること、
 (C)該食品がゲル状組織であること、
 (D)加熱処理済みであること。」が提案されている。
 特公昭49-1860号公報には「アルギン酸のアルカリ塩、抑制剤、脂肪及びアルカリ土類金属塩を混合して反応させ、脂肪をとり入れたアルギネートゲルの母組織を形成することを特徴とする人造の脂肪組織を製造する方法。」が提案されている。
For example, Japanese Patent No. 6265121 proposes "an oil-in-water emulsion soy protein gel food obtained by gelling an oil-in-water emulsion slurry containing 10 to 60% by weight of oil droplets having a particle size of 50 μm to 800 μm, wherein the planar occupancy rate of the oil droplets having a diameter of 50 μm to 800 μm is 10 to 60%, a gel made of a non-myosin gelling material exists as a continuous phase, and the gel is cross-linked by a protein cross-linking enzyme."
JP 2014-87316 A describes a food product similar to unheated animal tissue having the following characteristics (A) to (D).
(A) The food contains vegetable protein, fats and oils, and water, and the fats and oils are dispersed in an incompletely emulsified state;
(B) The food contains a protein that has been intermolecularly cross-linked by the action of a protein cross-linking enzyme;
(C) the food product is a gel-like tissue;
(D) It has been heat-treated."
Japanese Patent Publication No. 1860/1974 proposes "a method for producing artificial adipose tissue, characterized in that an alkaline salt of alginic acid, an inhibitor, fat and an alkaline earth metal salt are mixed and reacted to form an alginate gel base tissue incorporating fat."

 しかし、従来の脂身に似た食感を有する食材は、畜肉に含まれる脂身に似た食感を再現するためには不十分であった。
 そこで、本開示の実施形態の課題は、畜肉に含まれる脂身に似た食感を有する脂肪塊組成物、及び畜肉に含まれる脂身に似た食感を有する脂肪塊組成物を含む代替肉を提供することである。
However, conventional food ingredients that have a texture similar to that of fat have been inadequate for recreating the texture similar to that of fat contained in meat.
Therefore, an objective of an embodiment of the present disclosure is to provide a fat chunk composition having a texture similar to the fat contained in livestock meat, and a meat substitute comprising a fat chunk composition having a texture similar to the fat contained in livestock meat.

 本開示は、以下の態様を含む。
<1> 植物性油脂と、
 イオン架橋性ポリマーの多価金属塩と、
 グルコマンナンのゲル化物と、を含有する脂肪塊組成物。
<2> イオン架橋性ポリマーがアルギン酸又はLMペクチンである<1>に記載の脂肪塊組成物。
<3> 植物性油脂が、アルギン酸又はLMペクチンの多価金属塩及びグルコマンナンのゲル化物で覆われている<2>に記載の脂肪塊組成物。
<4> 多重バイト試験におけるToughnessが1000gw・cm/cm以上10000gw・cm/cm以下である<1>~<3>のいずれか1つに記載の脂肪塊組成物。
<5> タンパク質を含む赤身様部分と、
 <1>~<4>のいずれか1つに記載の脂肪塊組成物と、を有する代替肉。
<6> 赤身様部分と、脂肪塊組成物と、の界面に大豆タンパク質の粉状体を含有する<5>に記載の代替肉。
<7> 赤身様部分がスポンジ質又は繊維質である<5>又は<6>に記載の代替肉。
<8> 植物性油脂と、イオン架橋性ポリマーを含む水溶液と、を乳化して乳化物を得る工程と、
 乳化物と、グルコマンナン及び水酸化カルシウムを含む溶液と、を混合して混合液を得る工程と、
 混合液を加熱する工程と、を含む脂肪塊組成物の製造方法。
<9> <8>に記載の製造方法で得られた脂肪塊組成物の一部に粉末状の大豆タンパク質を付着し、脂肪塊組成物の粉末状の大豆タンパク質を付着した領域にタンパク質を含む赤身様部分を接触させる工程を含む<5>~<7>のいずれか1つに記載の代替肉の製造方法。
The present disclosure includes the following aspects.
<1> Vegetable oil and fat,
A polyvalent metal salt of an ionically crosslinkable polymer;
A fat mass composition comprising a gelled product of glucomannan.
<2> The fat mass composition described in <1>, wherein the ionically crosslinkable polymer is alginic acid or LM pectin.
<3> A fat block composition described in <2>, in which the vegetable oil is covered with a polyvalent metal salt of alginic acid or LM pectin and a gel of glucomannan.
<4> A fat block composition according to any one of <1> to <3>, having a toughness of 1000 gw·cm/cm2 or more and 10000 gw·cm/ cm2 or less in a multiple bite test.
<5> A red meat-like portion containing protein;
A meat substitute comprising the fat chunk composition described in any one of <1> to <4>.
<6> The alternative meat described in <5>, which contains soy protein powder at the interface between the red meat-like portion and the fat block composition.
<7> The alternative meat according to <5> or <6>, wherein the red meat-like portion is spongy or fibrous.
<8> A step of emulsifying a vegetable oil and an aqueous solution containing an ionically crosslinkable polymer to obtain an emulsion;
A step of mixing the emulsion with a solution containing glucomannan and calcium hydroxide to obtain a mixed liquid;
A method for producing a fat chunk composition comprising the step of heating the mixed liquid.
<9> A method for producing an alternative meat described in any one of <5> to <7>, comprising a step of attaching powdered soy protein to a part of the fat chunk composition obtained by the manufacturing method described in <8>, and contacting a red meat-like part containing protein with the area of the fat chunk composition to which the powdered soy protein has been attached.

 本開示の実施形態によれば、畜肉に含まれる脂身に似た食感を有する脂肪塊組成物、及び脂肪塊組成物の製造方法、並びに、畜肉に含まれる脂身に似た食感を有する脂肪塊組成物を含む代替肉及び代替肉の製造方法が提供される。 According to an embodiment of the present disclosure, there is provided a fat chunk composition having a texture similar to the fat contained in livestock meat, a method for producing the fat chunk composition, and a meat substitute containing a fat chunk composition having a texture similar to the fat contained in livestock meat and a method for producing the meat substitute.

本開示に係る代替肉の試作例である。1 is a prototype of a meat substitute according to the present disclosure.

 以下、本開示の一例である実施形態について説明する。これらの説明および実施例は、実施形態を例示するものであり、発明の範囲を制限するものではない。
 本明細書中に段階的に記載されている数値範囲において、一つの数値範囲で記載された上限値又は下限値は、他の段階的な記載の数値範囲の上限値又は下限値に置き換えてもよい。また、本明細書中に記載されている数値範囲において、その数値範囲の上限値又は下限値は、実施例に示されている値に置き換えてもよい。
 各成分は該当する物質を複数種含んでいてもよい。
 組成物中の各成分の量について言及する場合、組成物中に各成分に該当する物質が複数種存在する場合には、特に断らない限り、組成物中に存在する当該複数種の物質の合計量を意味する。
 「工程」とは、独立した工程だけではなく、他の工程と明確に区別できない場合であってもその工程の所期の作用が達成されれば、本用語に含まれる。
 本明細書において、2以上の好ましい態様の組み合わせは、より好ましい態様である。
Hereinafter, an embodiment that is an example of the present disclosure will be described. These descriptions and examples are merely illustrative of the embodiment, and are not intended to limit the scope of the present invention.
In the present specification, the upper or lower limit of one numerical range may be replaced with the upper or lower limit of another numerical range. In addition, in the present specification, the upper or lower limit of the numerical range may be replaced with a value shown in the examples.
Each component may contain multiple types of the corresponding substance.
When referring to the amount of each component in a composition, if the composition contains multiple substances corresponding to each component, the amount refers to the total amount of those multiple substances present in the composition, unless otherwise specified.
The term "process" includes not only independent processes, but also processes that cannot be clearly distinguished from other processes as long as the intended effect of the process is achieved.
As used herein, a combination of two or more preferred aspects is a more preferred aspect.

<脂肪塊組成物>
 本実施形態に係る脂肪塊組成物は、植物性油脂と、イオン架橋性ポリマーの多価金属塩
と、グルコマンナンのゲル化物と、を含有する。
 本実施形態に係る脂肪塊組成物は、上記構成により、畜肉に含まれる脂身に似た食感を有する。その理由は、次の通り推測される。
<Fat chunk composition>
The fat mass composition of this embodiment contains vegetable oil, a polyvalent metal salt of an ionically crosslinkable polymer, and a gel of glucomannan.
The fat chunk composition according to the present embodiment has a texture similar to that of fat contained in livestock meat due to the above-mentioned composition. The reason for this is presumed to be as follows.

 脂肪塊組成物が、植物性油脂及びイオン架橋性ポリマーの多価金属塩を含有することで、脂肪塊組成物中に脂肪性油脂を含む硬化物が得られる。そして、脂肪塊組成物は、更にグルコマンナンのゲル化物を含有する。グルコマンナンのゲル化物は、畜肉に含まれる脂身に近い弾力を発現する。また脂肪塊組成物は咀嚼により構造体が壊れ、内部に含まれる脂肪性油脂を染み出す。
 以上のことから、本開示に係る脂肪塊組成物は、弾力を有し、咀嚼時に油脂が滲みだす特性を有する。
The fat lump composition contains a vegetable oil and a polyvalent metal salt of an ionically crosslinkable polymer, so that a hardened material containing fatty oils and fats can be obtained in the fat lump composition. The fat lump composition further contains a gel of glucomannan. The glucomannan gel exhibits elasticity similar to that of fat contained in livestock meat. Furthermore, the structure of the fat lump composition breaks down when chewed, causing the fatty oils and fats contained therein to seep out.
From the above, the fat block composition according to the present disclosure has elasticity and has the property of causing fats and oils to seep out when chewed.

 そのため、本実施形態に係る脂肪塊組成物は畜肉に含まれる脂身に似た食感を有する。 As a result, the fat chunk composition of this embodiment has a texture similar to that of fat contained in livestock meat.

(植物性油脂)
 植物性油脂としては、例えば、ナタネ油、大豆油、パーム油、オリーブ油、米油、コーン油、ココナッツ油などが挙げられる。
 植物性油脂とは、植物を原料として得られたものであることを指す。
(Vegetable oils and fats)
Examples of vegetable oils include rapeseed oil, soybean oil, palm oil, olive oil, rice bran oil, corn oil, and coconut oil.
Vegetable oils and fats refer to those obtained using plants as raw materials.

 植物性油脂の融点は特に限定されないが、例えば-20℃以上300℃以下であってもよい。 The melting point of the vegetable oil is not particularly limited, but may be, for example, between -20°C and 300°C.

 油脂の融点は、熱分析測定装置によって測定される値である。
 熱分析測定装置としては、例えば、セイコー電子工業社製SSC5000DSC200が使用可能である。
 油脂の融点の測定は、試料3mgを装置に加え、昇温速度3℃/minにて測定する。
The melting point of the fat or oil is a value measured by a thermal analysis measuring device.
As the thermal analysis measuring device, for example, SSC5000DSC200 manufactured by Seiko Electronic Industries Co., Ltd. can be used.
The melting point of the oil or fat is measured by adding 3 mg of a sample to the apparatus and raising the temperature at a rate of 3° C./min.

 植物性油脂の含有量は、脂肪塊組成物全体に対して、1質量%以上50質量%以下であることが好ましく、5質量%以上40質量%以下であることがより好ましく、10質量%以上35質量%以下であることが更に好ましい。 The vegetable oil content is preferably 1% by mass or more and 50% by mass or less, more preferably 5% by mass or more and 40% by mass or less, and even more preferably 10% by mass or more and 35% by mass or less, based on the total fat mass composition.

 植物性油脂は1種でもよいし、2種以上の組合せでもよい。 The vegetable oil may be one type or a combination of two or more types.

(イオン架橋性ポリマーの多価金属塩)
 「イオン架橋性ポリマー」とは、イオンとの反応により架橋するポリマーを意味する。
 イオン架橋性ポリマーは可食性のイオン架橋性ポリマーであることが好ましい。
 ここで、「可食性」とは、ヒトが経口摂取した際に健康状態に対して悪影響を及ぼさない性質を意味する。
(Polyvalent metal salt of ionically crosslinkable polymer)
By "ionically crosslinkable polymer" is meant a polymer that crosslinks upon reaction with ions.
Preferably, the ionically cross-linkable polymer is an edible ionically cross-linkable polymer.
Here, "edible" means the property of not having adverse effects on human health when orally ingested.

 イオン架橋性ポリマーとしては、カルボキシ基、カルボン酸陰イオン基(-COO)、スルホ基、及びスルホン酸陰イオン基(-SO )からなる群より選ばれる少なくとも一つを有する多糖類が挙げられる。
 イオン架橋性ポリマーとしては、例えば、アルギン酸、カラギナン、LMペクチン、HMペクチン、ジェランガム等が挙げられ、アルギン酸、及びLMペクチンからなる群から選択される少なくとも1種が好ましい。
 なお、「LM」は「低メトキシル(Low methoxyl)」の略称であり、「HM」は「高メトキシル(High methoxyl)」の略称である。
The ionically crosslinkable polymer may be a polysaccharide having at least one group selected from the group consisting of a carboxy group, a carboxylate anion group ( --COO.sup.- ), a sulfo group, and a sulfonate anion group ( --SO.sub.3.sup.- ).
Examples of the ionically crosslinkable polymer include alginic acid, carrageenan, LM pectin, HM pectin, gellan gum, etc., and at least one selected from the group consisting of alginic acid and LM pectin is preferred.
It should be noted that "LM" is an abbreviation for "Low methoxyl" and "HM" is an abbreviation for "High methoxyl."

 イオン架橋性ポリマーの1質量%水溶液(水溶液全体に対して、イオン架橋性ポリマーを1質量%含有する水溶液)における粘度は、0.01mPa・s以上3000mPa・
s以下であることが好ましく、0.1mPa・s以上2000mPa・s以下であることがより好ましく、1mPa・s以上1000mPa・s以下であることが更に好ましい。
The viscosity of a 1% by mass aqueous solution of the ionically crosslinkable polymer (an aqueous solution containing 1% by mass of the ionically crosslinkable polymer relative to the entire aqueous solution) is 0.01 mPa·s or more and 3000 mPa·s or more.
The viscosity is preferably 0.1 mPa·s or less, more preferably 0.1 mPa·s or more and 2000 mPa·s or less, and further preferably 1 mPa·s or more and 1000 mPa·s or less.

 イオン架橋性ポリマーの1質量%水溶液の粘度は、粘度計により測定される。測定条件は、例えば、下記の通りである。
<条件>
・粘度計:TVB10形(東機産業)
・測定温度:20℃
The viscosity of a 1% by mass aqueous solution of the ionically crosslinkable polymer is measured using a viscometer. The measurement conditions are, for example, as follows.
<Conditions>
Viscometer: TVB10 type (Toki Sangyo)
Measurement temperature: 20°C

 グルコマンナンのゲル化物との親和性を高め、より畜肉に含まれる脂身に似た食感を有する脂肪塊組成物とする観点から、イオン架橋性ポリマーとしてはアルギン酸又はLMペクチンを用いることが好まく、アルギン酸を用いることが更に好ましい。
 アルギン酸とは、多糖類の1種であり、マンヌロン酸及びその異性体であるグルクロン酸の脱水重合体である。
 アルギン酸の多価金属塩は、アルギン酸が有するカルボン酸陰イオン(-COO)と多価金属イオンとがイオン結合を形成している塩である。そして、多価金属イオンは複数のカルボン酸陰イオンとイオン結合を形成するため、アルギン酸の多価金属塩はアルギン酸が多価金属イオンを介して架橋する構造を有する。
From the viewpoint of increasing the affinity with the gelled glucomannan and producing a fat block composition having a texture more similar to that of the fat contained in livestock meat, it is preferable to use alginic acid or LM pectin as the ionically cross-linkable polymer, and it is even more preferable to use alginic acid.
Alginic acid is a type of polysaccharide, which is a dehydrated polymer of mannuronic acid and its isomer, glucuronic acid.
Polyvalent metal salts of alginic acid are salts in which ionic bonds are formed between the carboxylate anion ( -COO- ) of alginic acid and a polyvalent metal ion. Since the polyvalent metal ion forms ionic bonds with multiple carboxylate anions, the polyvalent metal salts of alginic acid have a structure in which alginic acid is crosslinked via the polyvalent metal ions.

 多価金属イオンは、イオン価数が2価以上の金属イオンであることが好ましい。
 多価金属イオンとしては、例えば、カルシウムイオン、マグネシウムイオン、鉄イオン(II)、銅イオン(II)、亜鉛イオン、マンガンイオン等の2価金属イオン;アルミニウムイオン、鉄イオン(III)等の3価金属イオンが挙げられる。
 畜肉に含まれる脂身に似た食感を有する脂肪塊組成物を得る観点から、多価金属イオンとしてはカルシウムイオン、マグネシウムイオン、及び亜鉛イオンから選択される少なくとも1種であることが好ましく、カルシウムイオンであることがより好ましい。
The polyvalent metal ion is preferably a metal ion having an ionic valence of divalent or higher.
Examples of polyvalent metal ions include divalent metal ions such as calcium ion, magnesium ion, iron (II), copper (II), zinc ion, and manganese ion; and trivalent metal ions such as aluminum ion and iron (III) ion.
From the viewpoint of obtaining a fat block composition having a texture similar to that of the fat contained in livestock meat, it is preferable that the polyvalent metal ion is at least one selected from calcium ions, magnesium ions, and zinc ions, and it is more preferable that the polyvalent metal ion is calcium ions.

 イオン架橋性ポリマーの多価金属塩としては、アルギン酸カルシウム、カラギナンカルシウム、LMペクチンカルシウム、HMペクチンカルシウム、ジェランガムカルシウム等が挙げられ、アルギン酸カルシウム、LMペクチンカルシウムが好ましい。 Examples of polyvalent metal salts of ionically cross-linkable polymers include calcium alginate, calcium carrageenan, LM pectin calcium, HM pectin calcium, gellan gum calcium, etc., with calcium alginate and LM pectin calcium being preferred.

 イオン架橋性ポリマーの含有量は、脂肪塊組成物全体に対して、0.001質量%以上5質量%以下であることが好ましく、0.01質量%以上4質量%以下であることがより好ましく、0.1質量%以上3質量%以下であることが更に好ましい。 The content of the ionically cross-linkable polymer is preferably 0.001% by mass or more and 5% by mass or less, more preferably 0.01% by mass or more and 4% by mass or less, and even more preferably 0.1% by mass or more and 3% by mass or less, based on the total fat mass composition.

 多価金属イオンは、例えば、アルギン酸ナトリウムなどのアルギン酸の1価金属塩を含む水溶液を、多価金属イオンを含有する水溶液中で撹拌することで得られる。 Polyvalent metal ions can be obtained, for example, by stirring an aqueous solution containing a monovalent metal salt of alginic acid, such as sodium alginate, in an aqueous solution containing polyvalent metal ions.

(グルコマンナンのゲル化物)
 グルコマンナンとは、主に、サトイモ科コンニャク(Amonphophallus 
 Konjac)の根茎から分離して得られる多糖類であり、グルコース及びマンノースで構成される。
 具体的には、グルコマンナンとは、D-グルコースとD-マンノースとが2:3の割合でβ-1,4結合している多糖類である。
(Glucomannan gel)
Glucomannan is a type of konjac (Amonphophallus konjac), which is a type of konjac that is grown in the Araceae family.
It is a polysaccharide isolated from the rhizome of the plant, Cannabis sativa (Konnjac), and is composed of glucose and mannose.
Specifically, glucomannan is a polysaccharide in which D-glucose and D-mannose are bonded via β-1,4 bonds in a ratio of 2:3.

 グルコマンナンは水に溶かして、アルカリを添加することによりゲル化する。
 本開示に係る脂肪塊組成物は、上述の様にして得られるグルコマンナンのゲル化物を含む。
 ここで、グルコマンナンのゲル化物は、少なくとも、グルコマンナン、及び水を含有し、弾性固体としての挙動を示すものを指す。
Glucomannan is dissolved in water and gelled by adding alkali.
The fat mass composition according to the present disclosure comprises a glucomannan gel obtained as described above.
Here, the glucomannan gel refers to one that contains at least glucomannan and water and behaves as an elastic solid.

 グルコマンナンの含有量は、脂肪塊組成物全体に対して、0.01質量%以上7質量%以下であることが好ましく、0.1質量%以上6質量%以下であることがより好ましく、1質量%以上5質量%以下であることが更に好ましい。 The glucomannan content is preferably 0.01% by mass or more and 7% by mass or less, more preferably 0.1% by mass or more and 6% by mass or less, and even more preferably 1% by mass or more and 5% by mass or less, based on the total fat mass composition.

 グルコマンナンのゲル化物の含有量は、脂肪塊組成物全体に対して、10質量%以上90質量%以下であることが好ましく、15質量%以上80質量%以下であることがより好ましく、25質量%以上75質量%以下であることが更に好ましい。 The content of the glucomannan gel is preferably 10% by mass or more and 90% by mass or less, more preferably 15% by mass or more and 80% by mass or less, and even more preferably 25% by mass or more and 75% by mass or less, based on the total fat mass composition.

(その他の添加剤)
 脂肪塊組成物は、植物性油脂、イオン架橋性ポリマーの多価金属塩、及びグルコマンナンのゲル化物以外のその他の添加剤を含有してもよい。
 その他の添加剤としては、例えば、調味料、酸味料、苦味料、香辛料、甘味料、酸化防止剤、着色料、発色料、香料、安定剤、保存料等が挙げられる。
 その他の添加剤の含有量としては、0質量以上10質量%以下であることが好ましい。
(Other additives)
The fat mass composition may contain additives other than the vegetable oil, the polyvalent metal salt of an ionically crosslinkable polymer, and the glucomannan gelatinized product.
Examples of other additives include seasonings, acidulants, bittering agents, spices, sweeteners, antioxidants, colorants, color formers, fragrances, stabilizers, preservatives, etc.
The content of the other additives is preferably from 0 to 10% by mass.

(脂肪塊組成物の態様)
 脂肪塊組成物は、植物性油脂が、アルギン酸多価金属塩及びグルコマンナンのゲル化物で覆われていることが好ましい。
 ここで、植物性油脂が、アルギン酸多価金属塩及びグルコマンナンのゲル化物で覆われているとは、アルギン酸多価金属塩及びグルコマンナンのゲル化物が、植物性油脂が染み出さない程度に植物性油脂を内部に含むことをいう。
 畜肉に含まれる脂身は、内部に油脂を含む脂肪細胞を含む。本開示に係る脂肪塊組成物を上述の構成とすることで、脂肪塊組成物の構造が、畜肉に含まれる脂身の構造と類似した構造となるため、より畜肉に含まれる脂身に似た食感を有する脂肪塊組成物となりやすい。
(Embodiments of fat chunk composition)
The fat mass composition preferably comprises vegetable oil covered with a gel of polyvalent metal alginate and glucomannan.
Here, the phrase "vegetable oil is covered with a gel of polyvalent metal alginate and glucomannan" means that the gel of polyvalent metal alginate and glucomannan contains vegetable oil inside to the extent that the vegetable oil does not seep out.
Fat contained in livestock meat contains fat cells containing fats and oils inside. By configuring the fat chunk composition according to the present disclosure as described above, the structure of the fat chunk composition becomes similar to the structure of the fat contained in livestock meat, and the fat chunk composition is likely to have a texture more similar to that of the fat contained in livestock meat.

 植物性油脂は球状に近い状態(以下、「油滴」と称する)で脂肪塊組成物中に多数分散していることがより好ましい。 It is more preferable that the vegetable oil is dispersed in large numbers in the fat mass composition in a nearly spherical form (hereinafter referred to as "oil droplets").

 畜肉に含まれる脂身は、多数の脂肪細胞を含んで構成されている。よって、畜肉に含まれる脂身は、多数の油滴を含んだ状態である。本開示に係る脂肪塊組成物を上述の構成とすることで、脂肪塊組成物の構造が、更に畜肉に含まれる脂身の構造と類似した構造となるため、更に畜肉に含まれる脂身に似た食感を有する脂肪塊組成物となりやすい。 The fat contained in livestock meat is composed of numerous fat cells. Therefore, the fat contained in livestock meat contains numerous oil droplets. By configuring the fat chunk composition according to the present disclosure as described above, the structure of the fat chunk composition becomes similar to the structure of the fat contained in livestock meat, and the fat chunk composition is more likely to have a texture similar to that of the fat contained in livestock meat.

(脂肪塊組成物の硬さ)
 脂肪塊組成物の硬さは、多重バイト試験におけるToughness(靭性)によって評価する。多重バイト試験におけるToughnessが1000(gw・cm/cm)以上10000(gw・cm/cm)以下であることが好ましく、1500(gw・cm/cm)以上8000(gw・cm/cm)以下であることがより好ましく、2000(gw・cm/cm)以上6000(gw・cm/cm)以下であることが更に好ましい。
 脂肪塊組成物の硬さ(多重バイト試験におけるToughness)を上記数値範囲内とすることで、脂肪塊組成物が畜肉に含まれる脂身に近い弾力を有しやすくなる。そのため、より畜肉に含まれる脂身に似た食感を有する脂肪塊組成物となりやすく、噛み応えが良好となりやすい。
(Hardness of fat mass composition)
The hardness of the fat block composition is evaluated by its toughness in a multiple bite test. The toughness in the multiple bite test is preferably from 1000 (gw·cm/ cm2 ) to 10,000 (gw·cm/ cm2 ), more preferably from 1500 (gw·cm/ cm2 ) to 8,000 (gw·cm/ cm2 ), and even more preferably from 2000 (gw·cm/ cm2 ) to 6,000 (gw·cm/ cm2 ).
By setting the hardness of the fat block composition (toughness in a multiple bite test) within the above numerical range, the fat block composition tends to have elasticity similar to that of the fat contained in livestock meat. Therefore, the fat block composition tends to have a texture more similar to that of the fat contained in livestock meat, and tends to have a good chewability.

 多重バイト試験におけるToughnessは、粘弾性試験装置で測定される値である。粘弾性試験装置としては、例えば、タケモト電機社製、品名:テンシプレッサーMyBpy2systemを使用することができる。
 以下、多重バイト試験におけるToughnessの測定方法について、具体的に説明する。
 多重バイト試験におけるToughnessの測定方法は、多重バイト試験を用いる。サンプルを30mm角で厚み5mmの大きさに裁断する。粘弾性試験装置のステージにセットし、多重バイト試験の測定条件にて3回ずつ測定し、その平均値を測定値とする。
The toughness in the multiple bite test is a value measured by a viscoelasticity tester. As the viscoelasticity tester, for example, a Tensipressor MyBpy2 system manufactured by Takemoto Denki Co., Ltd. can be used.
The method for measuring toughness in the multiple bite test will now be described in detail.
The toughness is measured by the multiple bite test. The sample is cut into a size of 30 mm square and 5 mm thick. The sample is set on the stage of the viscoelasticity tester and measured three times under the measurement conditions of the multiple bite test. The average value is taken as the measured value.

<代替肉>
 代替肉は、タンパク質を含む赤身様部分と、脂肪塊組成物と、を有することが好ましい。
 ここで、脂肪塊組成物としては、既述の脂肪塊組成物が適用される。
<Meat substitutes>
The substitute meat preferably has a lean meat-like portion containing protein and a fat mass composition.
Here, as the fat chunk composition, the fat chunk composition already described is applied.

(赤身様部分)
 赤身様部分とは、代替肉中の、赤身に見える部分に相当する部分を指す。
 赤身様部分は、タンパク質を含有し、必要に応じて、油脂、結着剤、及びその他の添加剤を含有することが好ましい。
(Red meat part)
The lean meat-like portion refers to the portion of the alternative meat that corresponds to the portion that looks like lean meat.
The lean meat-like portion preferably contains protein, and, if necessary, contains fats and oils, binders, and other additives.

-タンパク質-
 赤身様部分は、タンパク質を含有する。
 タンパク質は、植物性タンパク質及び動物性タンパク質の少なくとも1種を含有することが好ましく、植物性タンパク質を含有することがより好ましい。
-protein-
The lean portion contains protein.
The protein preferably contains at least one of a vegetable protein and an animal protein, and more preferably contains a vegetable protein.

 植物性タンパク質とは、植物から採取されるタンパク質である。
 植物性タンパク質としては、植物から採取されるタンパク質であれば特に限定されない。植物性タンパク質の由来としては、例えば、小麦、大麦、オーツ麦、米、トウモロコシ等の穀類;大豆、えんどう豆、小豆、ひよこ豆、レンズ豆、そら豆、緑豆、ハウチワ豆等の豆類; アーモンド、落花生、カシューナッツ、ピスタチオ、ヘーゼルナッツ、マカデミアンナッツ、アマニ、ゴマ、菜種、綿実、サフラワー、向日葵等の種実類;じゃがいも、さつまいも、山のいも、きくいも、キャッサバ等のいも類;アスパラガス、アーティチョーク、カリフラワー、ブロッコリー、枝豆等の野菜類;バナナ、ジャックフルーツ、キウイフルーツ、ココナッツ、アボカド、オリーブ等の果実類;マッシュルーム、エリンギ、しいたけ、しめじ、まいたけ等のきのこ類;クロレラ、スピルリナ、ユーグレナ、のり、こんぶ、わかめ、ひじき、てんぐさ、もずく等の藻類等が挙げられる。これらの中でも、塊肉に似た外観及び食感を有する生肉様代替肉を得る観点から、食部タンパク質の由来としては、小麦、大豆、えんどう豆、及び米からなる群から選択される少なくとも1種であることが好ましく、大豆及び小麦からなる群から選択される少なくとも1種であることがより好ましい。
 植物性タンパク質は、1種の植物由来のタンパク質を含有してもよいし、2種以上の植物由来のタンパク質を含有してもよい。
Vegetable protein is protein obtained from plants.
The vegetable protein is not particularly limited as long as it is a protein extracted from a plant. Examples of sources of vegetable proteins include grains such as wheat, barley, oats, rice, and corn; beans such as soybeans, peas, red beans, chickpeas, lentils, fava beans, mung beans, and lupins; nuts and seeds such as almonds, peanuts, cashew nuts, pistachios, hazelnuts, macadamia nuts, flaxseed, sesame, rapeseed, cottonseed, safflower, and sunflowers; tubers such as potato, sweet potato, mountain yam, Jerusalem artichoke, and cassava; vegetables such as asparagus, artichoke, cauliflower, broccoli, and edamame; fruits such as bananas, jackfruit, kiwi fruit, coconut, avocado, and olives; mushrooms such as mushrooms, king oyster mushrooms, shiitake mushrooms, shimeji mushrooms, and maitake mushrooms; and algae such as chlorella, spirulina, euglena, nori, kelp, wakame seaweed, hijiki, tengusa, and mozuku seaweed. Among these, from the viewpoint of obtaining a raw meat-like meat substitute having an appearance and texture similar to that of a chunk of meat, the origin of the edible protein is preferably at least one selected from the group consisting of wheat, soybeans, peas, and rice, and more preferably at least one selected from the group consisting of soybeans and wheat.
The vegetable protein may contain a protein derived from one type of plant, or may contain proteins derived from two or more types of plants.

 動物性タンパク質とは、動物から採取されるタンパク質である。動物性タンパク質は、動物から採取してもよく、動物から採取されるタンパク質と同じアミノ酸配列のタンパク質を細胞培養又は酵素反応によって生産し抽出してもよい。
 動物性タンパク質としては、動物から採取されるタンパク質であれば特に限定されない。動物性タンパク質としては、例えば、コラーゲン、ゼラチン、ケラチン、フィブロイン、セリシン、カゼイン、コンキオリン、エラスチン、プロタミン、卵黄タンパク質、卵白タンパク質等が挙げられる。
 動物性タンパク質は、1種のみを含有してもよいし、2種以上含有してもよい。
Animal protein is a protein obtained from an animal. The animal protein may be obtained from an animal, or a protein having the same amino acid sequence as the protein obtained from an animal may be produced by cell culture or enzyme reaction and extracted.
The animal protein is not particularly limited as long as it is a protein obtained from an animal, and examples of the animal protein include collagen, gelatin, keratin, fibroin, sericin, casein, conchiolin, elastin, protamine, egg yolk protein, and egg white protein.
The animal protein may contain only one type, or may contain two or more types.

 より塊肉に近い食感を有する生肉様代替肉を得る観点から、タンパク質は筋肉様の組織を有していることが好ましい。
 ここで筋肉様の組織とは、繊維の束に類似した構造を有し、一定の方向に繊維状に割くことができる組織をいう。
 畜肉の赤身は筋肉に由来する。そして筋肉は、筋繊維の束により構成される。よって畜肉の赤身は繊維の束のような構造を有している。本実施形態に係る生肉様代替の赤身様部分が含有するタンパク質が、筋肉様の組織を有することで、畜肉を食した際に感じられる筋繊維の存在によってもたらされる食感の演出が可能となる。
In order to obtain a raw meat-like meat substitute having a texture closer to that of whole meat, it is preferable that the protein has a muscle-like tissue.
Here, muscle-like tissue refers to tissue that has a structure similar to a bundle of fibers and can be split into fibers in a certain direction.
Red meat of livestock originates from muscle. And muscle is composed of muscle fiber bundles. Therefore, red meat of livestock has a structure like a fiber bundle. By having the protein contained in the red meat-like portion of the raw meat substitute according to this embodiment have muscle-like tissue, it is possible to produce the texture brought about by the presence of muscle fiber felt when eating livestock meat.

 タンパク質が筋肉様の組織を有するようにする方法としては、タンパク質(必要に応じて、タンパク質と共に水等を添加してもよい)を押出機によって押出成形する方法が挙げられる。
 タンパク質を押出方向に剪断応力をかけながら押出成形することで、タンパク質は押出機の押出方向に沿った繊維の束に類似した構造を有し、押出機の押出方向に繊維状に割くことができる組織を有する。
As a method for making a protein have a muscle-like structure, a method of extruding a protein (water or the like may be added together with the protein, if necessary) using an extruder can be mentioned.
By extruding a protein while applying a shear stress in the extrusion direction, the protein has a structure similar to a bundle of fibers aligned in the extrusion direction of the extruder, and has a texture that can be split into fibers in the extrusion direction of the extruder.

 タンパク質の含有量は、赤身様部分全体に対して、50質量%以上100質量%以下であることが好ましく、60質量%以上95質量%以下であることがより好ましく、70質量%以上90質量%以下であることが更に好ましい。 The protein content of the entire red meat-like portion is preferably 50% to 100% by mass, more preferably 60% to 95% by mass, and even more preferably 70% to 90% by mass.

-油脂-
 赤身様部分は、油脂を含有してもよい。
 油脂は、液状の状態で含有されていてもよく、ゲル化や乳化などの方法で固体状態で含有されていてもよい。
 油脂としては、植物性油脂、動物性油脂などが挙げられる。
 植物性油脂としては、脂肪塊組成物の説明において述べたものと同一のものが挙げられる。
 動物性油脂としては、例えば、牛脂、豚脂、鯨脂、魚油などが挙げられる。
-Oils and fats-
The lean meat-like portion may contain fats and oils.
The oil or fat may be contained in a liquid state, or may be contained in a solid state by a method such as gelation or emulsification.
Examples of the oils and fats include vegetable oils and fats, animal oils and fats, and the like.
Examples of the vegetable oil include the same ones as those mentioned in the description of the fat mass composition.
Examples of animal fats and oils include beef tallow, lard, whale fat, and fish oil.

 赤身様部分に含有される油脂の含有量は、赤身様部分全体に対して、0質量%以上50質量%以下であることが好ましく、0質量%以上40質量%以下であることがより好ましく、0質量%以上30質量%以下であることが更に好ましい。 The fat content in the red meat-like portion is preferably 0% by mass or more and 50% by mass or less, more preferably 0% by mass or more and 40% by mass or less, and even more preferably 0% by mass or more and 30% by mass or less, based on the entire red meat-like portion.

-結着剤-
 赤身様部分は、必要に応じて結着剤を含有することが好ましい。
 赤身様部分が結着剤を含有することで、赤身様部分が一つのまとまった形状を維持しやすくなる。
- Binding agent -
The lean meat-like portion preferably contains a binder as necessary.
When the red meat-like portion contains a binder, the red meat-like portion can easily maintain a unified shape.

 結着剤としては、可食性であり、かつ赤身様部分の形状を維持できるものであれば特に限定されない。
 結着剤としては、例えば、タンパク質、増粘多糖類、澱粉、タンパク質を硬化させる酵素等が挙げられる。結着剤は単独で用いてもよく、これらを混合して用いてもよい。
 結着剤として用いられるタンパク質は、赤身様部分に含有されるタンパク質と同一であってもよいし、異なっていてもよい。
The binder is not particularly limited as long as it is edible and can maintain the shape of the red meat-like portion.
Examples of the binder include proteins, thickening polysaccharides, starch, enzymes that harden proteins, etc. The binders may be used alone or in combination.
The protein used as the binder may be the same as or different from the protein contained in the lean meat-like portion.

 結着剤として用いられるタンパク質としては、例えば、植物性タンパク質、動物性タンパク質などが挙げられる。
 結着剤として用いられる植物性タンパク質としては、例えば、小麦、大豆、米などを由来とするタンパク質が挙げられる。
 結着剤として用いられる動物性タンパク質としては、例えば、乳タンパク質、卵白などが挙げられる。
 タンパク質を硬化させる酵素としては、例えば、トランスグルタミナーゼなどが挙げられる。トランスグルタミナーゼは市販品を用いることができ、例えば、味の素株式会社製
 アクティバ(登録商標)シリーズが挙げられる。
Examples of proteins used as binders include vegetable proteins and animal proteins.
Examples of vegetable proteins used as binders include proteins derived from wheat, soybeans, rice, and the like.
Examples of animal proteins used as binders include milk proteins and egg whites.
Examples of enzymes that harden proteins include transglutaminase, etc. Commercially available transglutaminase products can be used, such as the Activa (registered trademark) series manufactured by Ajinomoto Co., Inc.

 増粘多糖類としては、例えば、カラギナン、キサンタンガム、ペクチン、ローカストビーンガム、カードラン、グアガム、トラガントガム、アラビアガム、ジェランガム、タマリンドシードガム、カシアガム、タラガム、アルギン酸、寒天、グルコマンナン、大豆多糖類、ゼラチン、プルラン、サイリウム、キトサン、メチルセルロース、ヒドロキシプロピルメチルセルロース、ヒドロキシエチルセルロース、カルボキシメチルセルロース、デキストリン等が挙げられる。 Thickening polysaccharides include, for example, carrageenan, xanthan gum, pectin, locust bean gum, curdlan, guar gum, tragacanth gum, gum arabic, gellan gum, tamarind seed gum, cassia gum, tara gum, alginic acid, agar, glucomannan, soybean polysaccharides, gelatin, pullulan, psyllium, chitosan, methylcellulose, hydroxypropylmethylcellulose, hydroxyethylcellulose, carboxymethylcellulose, dextrin, etc.

 澱粉としては、例えば、小麦澱粉、キャッサバ澱粉、米澱粉、もち米澱粉、コーンスターチ、ワキシーコーンスターチ、サゴ澱粉、馬鈴薯澱粉、葛澱粉、蓮根澱粉、緑豆澱粉、甘藷澱粉、ワキシー馬鈴薯澱粉、ワキシーキャッサバ澱粉、ワキシー小麦澱粉等が挙げられる。 Examples of starches include wheat starch, cassava starch, rice starch, glutinous rice starch, corn starch, waxy corn starch, sago starch, potato starch, kudzu starch, lotus root starch, mung bean starch, sweet potato starch, waxy potato starch, waxy cassava starch, waxy wheat starch, etc.

 赤身様部分に含有される結着剤の含有量は、赤身様部分全体に対して、0.01質量%以上30質量%以下であることが好ましく、0.1質量%以上25質量%以下であることがより好ましく、1質量%以上20質量%以下であることが更に好ましい。 The content of the binder contained in the red meat-like portion is preferably 0.01% by mass or more and 30% by mass or less, more preferably 0.1% by mass or more and 25% by mass or less, and even more preferably 1% by mass or more and 20% by mass or less, based on the entire red meat-like portion.

-その他の添加剤-
 赤身様部分は必要に応じて、タンパク質、油脂及び結着剤以外のその他の添加剤を含有することが好ましい。
 その他の添加剤としては、例えば、水、調味料、酸味料、苦味料、香辛料、甘味料、酸化防止剤、着色料、発色料、香料、安定剤、保存料等が挙げられる。
 その他の添加剤の含有量としては、0質量以上20質量%以下であることが好ましい。
-Other additives-
The lean meat-like portion preferably contains other additives other than the protein, fats and oils, and binders, as necessary.
Examples of other additives include water, seasonings, acidulants, bittering agents, spices, sweeteners, antioxidants, colorants, color formers, fragrances, stabilizers, preservatives, etc.
The content of the other additives is preferably from 0 to 20% by mass.

(赤身様部分の態様(1))
 赤身様部分はスポンジ質又は繊維質であることが好ましい。
 赤身様部分をスポンジ質又は繊維質とすることで、塊肉に似た食感を有する生肉様代替肉となりやすい。
(Embodiment of red meat-like portion (1))
The lean portion is preferably spongy or fibrous.
By making the lean meat-like portion spongy or fibrous, it becomes easier to produce a raw meat-like substitute that has a texture similar to that of a chunk of meat.

 ここでスポンジ質であるとは、外観上、当方的な多孔構造であることを指し、一方、繊維質とは、外観上、異方性を有した繊維的構造であることを指す。 In this context, "spongy" refers to a porous structure that appears isotropic, while "fibrous" refers to a fibrous structure that appears anisotropic.

 等方的な多孔構造とは、任意の切断面の孔形状が略楕円形状で方向によらず略同一である構造を示す。
 異方的な繊維構造とは、切断面の孔形状が略楕円状と略繊維状で方向によって異なる構造を示す。
The term "isotropic porous structure" refers to a structure in which the pore shape of any cross section is approximately elliptical and is approximately the same regardless of the direction.
The anisotropic fiber structure refers to a structure in which the pore shape in the cut section varies depending on the direction, being generally elliptical or generally fibrous.

 赤身様部分に含有されるタンパク質は、原料タンパク質及び水を押出機に加えて、混練及び押出しを行うことで製造することが好ましい。混練及び押出しの温度条件は、水の沸点以上で行うことが好ましいため、混錬直後に大気圧に押し出されるとタンパク質は水の沸騰により膨化し、タンパク質は多孔質構造を有し、赤身様部分はスポンジ状となる。
 一方、混錬直後に剪断応力を加えると、タンパク質が繊維状に配向しやすくなり、赤身様部分は繊維質となる。
The protein contained in the lean meat-like portion is preferably produced by adding raw material protein and water to an extruder and kneading and extruding. The temperature conditions for kneading and extruding are preferably above the boiling point of water, so that when the mixture is extruded to atmospheric pressure immediately after kneading, the protein swells due to the boiling of water, has a porous structure, and the lean meat-like portion becomes spongy.
On the other hand, if shear stress is applied immediately after kneading, the proteins tend to be oriented in a fibrous form, and the red meat-like portion becomes fibrous.

 赤身様部分を繊維質に制御する方法としては、例えば、赤身様部分に含有されるタンパク質を押出機から押し出す際に、ダイを使用するか否かによって制御する方法が挙げられる。
 例えば、赤身様部分に含有されるタンパク質を押出機から押し出す際に、ダイを使用すると、赤身様部分は繊維質となりやすい。
 具体的な赤身様部分の製造方法については後述する。
An example of a method for controlling the lean meat-like portion to be fibrous is to control the protein contained in the lean meat-like portion by using or not using a die when extruding the protein from an extruder.
For example, when a die is used to extrude the protein contained in the lean meat portion from an extruder, the lean meat portion tends to become fibrous.
A specific method for producing the lean meat-like portion will be described later.

 赤身様部分がスポンジ質又は繊維質であることにより、より塊肉に近い食感を有する生肉様代替肉となる。
 更に塊肉に近い食感を有する生肉様代替肉とする観点から、赤身様部分は繊維質であることが更に好ましい。
The lean meat-like portion is spongy or fibrous, resulting in a raw meat substitute that has a texture closer to that of a whole piece of meat.
Furthermore, from the viewpoint of producing a raw meat-like meat substitute having a texture similar to that of whole meat, it is further preferable that the lean meat-like portion is fibrous.

(代替肉の態様)
 赤身様部分と、脂肪塊組成物と、の界面に大豆タンパク質の粉状体を含有することが好ましい。
 当該構成とすることで、赤身様部分及び脂肪塊組成物の密着性が向上し、畜肉に似た外観をより維持できる代替肉となる。
(Aspects of Alternative Meat)
It is preferable to contain soy protein powder at the interface between the lean meat-like portion and the fat chunk composition.
This composition improves adhesion between the lean meat-like portion and the fat chunk composition, resulting in a meat substitute that can maintain an appearance more similar to that of livestock meat.

 大豆タンパク質の粉状体とは、大豆タンパク質を主成分として含む粉状体を指す。
 ここで「主成分」とは、該当する成分を大豆タンパク質の粉状体全体に対して90質量%以上含有することをいう。
Soy protein powder refers to a powder containing soy protein as the main component.
Here, the term "main component" refers to a soy protein powder containing the relevant component in an amount of 90% by mass or more based on the entire powder.

 大豆タンパク質の粉状体としては、例えば、フジプロFR(不二製油社製)、昭和フレッシュRF(昭和産業社製)、ソーヤフラワーFT-N(日清オイリオ社製)等が挙げられる。 Examples of soy protein powder include Fujipro FR (manufactured by Fuji Oil Co., Ltd.), Showa Fresh RF (manufactured by Showa Sangyo Co., Ltd.), and Soya Flour FT-N (manufactured by Nisshin Oillio Co., Ltd.).

 大豆タンパク質の粉状体が、赤身様部分と、脂肪塊組成物と、の界面に含まれているか否かについては、赤身様部分と、脂肪塊組成物と、の界面を顕微鏡で観察することで行う。
 具体的には、以下の手順にて行う。
 サンプルを凍結させ、赤身部分と脂肪塊組成物の界面に対して10°の角度でサンプルを切断し、断面のうち光学顕微鏡にて界面部分を観察する。脂肪塊組成物の成分と大豆タンパク質の粉状体の混合部分があることを観察する。
Whether or not soy protein powder is contained at the interface between the red meat-like portion and the fat chunk composition is determined by observing the interface between the red meat-like portion and the fat chunk composition under a microscope.
Specifically, the procedure is as follows.
The sample is frozen, cut at an angle of 10° to the interface between the lean meat portion and the fat chunk composition, and the interface portion of the cross section is observed under an optical microscope. It is observed that the components of the fat chunk composition and the soy protein powder are mixed.

<脂肪塊組成物の製造方法>
 脂肪塊組成物の製造方法は、植物性油脂と、イオン架橋性ポリマーを含む水溶液と、を乳化して乳化物を得る工程(乳化工程)と、上記乳化物と、グルコマンナン及び水酸化カルシウムを含む溶液と、を混合して混合液を得る工程(混合工程)と、上記混合液を加熱する工程(加熱工程)と、を含むことが好ましい。
<Method of producing fat chunk composition>
The method for producing a fat chunk composition preferably includes a step of emulsifying vegetable oil and an aqueous solution containing an ionically cross-linkable polymer to obtain an emulsion (emulsification step), a step of mixing the emulsion with a solution containing glucomannan and calcium hydroxide to obtain a mixed liquid (mixing step), and a step of heating the mixed liquid (heating step).

(乳化工程)
 乳化工程は、植物性油脂と、イオン架橋性ポリマーを含む水溶液と、を乳化して乳化物を得る工程である。
 乳化工程で用いられる植物性油脂としては、既述のものと同様のものが挙げられる。
(Emulsification process)
The emulsification step is a step of emulsifying a vegetable oil and an aqueous solution containing an ionically crosslinkable polymer to obtain an emulsion.
The vegetable oils and fats used in the emulsification step include those already mentioned.

 イオン架橋性ポリマーとしては、既述のものと同様のものが挙げられる。
 イオン架橋性ポリマーを含む溶液は、イオン架橋性ポリマーの1価金属塩を含む溶液であることが好ましい。
 イオン架橋性ポリマーの1価金属塩とは、イオン架橋性ポリマーが有するカルボン酸陰イオン(-COO)及びスルホン酸陰イオン基(-SO )の少なくとも一方と1価金属イオンとがイオン結合を形成している塩である。
 1価金属イオンとしては、アルカリ金属イオンが好ましく、リチウムイオン、ナトリウムイオン及びカリウムイオンがより好ましく、ナトリウムイオンが最も好ましい。
 イオン架橋性ポリマーの1価金属塩としては、具体的には、アルギン酸ナトリウムが挙
げられる。
The ionically crosslinkable polymer may be the same as those mentioned above.
The solution containing the ionically crosslinkable polymer is preferably a solution containing a monovalent metal salt of the ionically crosslinkable polymer.
The monovalent metal salt of an ionically crosslinkable polymer is a salt in which at least one of a carboxylate anion ( --COO.sup.- ) and a sulfonic acid anion group ( --SO.sub.3.sup.- ) contained in the ionically crosslinkable polymer forms an ionic bond with a monovalent metal ion.
As the monovalent metal ion, an alkali metal ion is preferred, a lithium ion, a sodium ion and a potassium ion are more preferred, and a sodium ion is most preferred.
A specific example of the monovalent metal salt of an ionically crosslinkable polymer is sodium alginate.

 水溶液中における、イオン架橋性ポリマーの含有量は、水溶液全体に対して0.1質量%以上10質量%以下であることが好ましい。 The content of the ionically cross-linkable polymer in the aqueous solution is preferably 0.1% by mass or more and 10% by mass or less based on the total amount of the aqueous solution.

 植物性油脂と、イオン架橋性ポリマーを含む水溶液と、の質量比(植物性油脂の質量/イオン架橋性ポリマーを含む水溶液の質量)は、1/5以上5/1以下であることが好ましい。
 植物性油脂と、イオン架橋性ポリマーを含む水溶液と、を乳化させる方法としては、特に限定されず、植物性油脂と、イオン架橋性ポリマーを含む水溶液と、を含む溶液を乳化機により乳化する方法が好ましい。
 乳化機としては、例えば、プロペラ型、アンカー型、パドル型、又はタービン型の撹拌羽根を備えた回転式攪拌機、スタティックミキサー等の静止型混合器、ホモジナイザー、クレアミックス等のローター・ステーター型乳化機、磨砕機能を備えたミル型乳化機、マントンゴーリン式圧力乳化機等の高圧乳化機、高圧下でキャビテーションを発生させる高圧ノズル型乳化機、マイクロフルイダイザー等の高圧下で液同士を衝突させることによりせん断力を与える高圧衝突型乳化機、超音波でキャビテーションを発生させる超音波乳化機、細孔を通して均一乳化を行う膜乳化機等が例示される。
The mass ratio of the vegetable oil to the aqueous solution containing the ionically crosslinkable polymer (mass of vegetable oil/mass of aqueous solution containing the ionically crosslinkable polymer) is preferably 1/5 or more and 5/1 or less.
The method for emulsifying the vegetable oil and the aqueous solution containing the ionically cross-linkable polymer is not particularly limited, and a method of emulsifying a solution containing the vegetable oil and the aqueous solution containing the ionically cross-linkable polymer using an emulsifier is preferred.
Examples of emulsifiers include rotary mixers equipped with propeller-type, anchor-type, paddle-type, or turbine-type stirring blades, static mixers such as static mixers, rotor-stator type emulsifiers such as homogenizers and Clearmix, mill-type emulsifiers equipped with a grinding function, high-pressure emulsifiers such as Manton-Gaulin pressure emulsifiers, high-pressure nozzle-type emulsifiers that generate cavitation under high pressure, high-pressure collision-type emulsifiers such as microfluidizers that apply shear force by colliding liquids together under high pressure, ultrasonic emulsifiers that generate cavitation using ultrasound, and membrane emulsifiers that perform uniform emulsification through fine pores.

 植物性油脂と、イオン架橋性ポリマーを含む水溶液と、を乳化させる際、可食性の界面活性剤を添加してもよい。
 可食性の界面活性剤としては、グリセリン脂肪酸エステル、ポリグリセリン脂肪酸エステル、有機酸モノグリセリド、ソルビタン脂肪酸エステル、プロピレングリコール脂肪酸エステル、ショ糖脂肪酸エステル、ポリグリセリン縮合リシノレイン酸エステル、レシチン等が挙げられる。
 界面活性剤を添加する際、乳化物全体に対する界面活性剤の含有量が、0.1質量%以上5質量%以下となる様に添加することが好ましい。
When emulsifying the vegetable oil and the aqueous solution containing the ionically crosslinkable polymer, an edible surfactant may be added.
Examples of edible surfactants include glycerin fatty acid esters, polyglycerin fatty acid esters, organic acid monoglycerides, sorbitan fatty acid esters, propylene glycol fatty acid esters, sucrose fatty acid esters, polyglycerin condensed ricinoleic acid esters, and lecithin.
When the surfactant is added, it is preferable to add the surfactant so that the content of the surfactant in the entire emulsion is 0.1% by mass or more and 5% by mass or less.

 植物性油脂が、アルギン酸多価金属塩及びグルコマンナンのゲル化物で覆われている構造としやすい観点から、乳化物は水中油型乳化物とすることが好ましい。 The emulsion is preferably an oil-in-water emulsion, since it is easy to form a structure in which the vegetable oil is covered with a gel of polyvalent metal alginate and glucomannan.

(混合工程)
 混合工程は、乳化工程を経て得られる乳化物と、グルコマンナン及び水酸化カルシウムを含む溶液と、混合して混合液を得る工程である。
(Mixing process)
The mixing step is a step of mixing the emulsion obtained through the emulsification step with a solution containing glucomannan and calcium hydroxide to obtain a mixed liquid.

 乳化工程を経て得られる乳化物は、イオン架橋性ポリマーを含有する。当該化合物は、多価金属イオンの存在下でイオン交換反応を起こし、ポリマーの架橋構造を生成する。混合工程においては当該反応が主に進行していると推測される。 The emulsion obtained through the emulsification process contains an ionically cross-linkable polymer. This compound undergoes an ion exchange reaction in the presence of polyvalent metal ions, producing a cross-linked polymer structure. It is assumed that this reaction mainly takes place during the mixing process.

 グルコマンナン及び水酸化カルシウムを含む溶液中の、グルコマンナンの含有量は、グルコマンナン及び水酸化カルシウムを含む溶液全体に対して、0.1質量%以上10質量%以下であることが好ましく、0.2質量%以上8質量%以下であることがより好ましく、0.5質量%以上5質量%以下であることが更に好ましい。
 グルコマンナン及び水酸化カルシウムを含む溶液中の、水酸化カルシウムの含有量は、グルコマンナン及び水酸化カルシウムを含む溶液全体に対して、0.01質量%以上1質量%以下であることが好ましく、0.02質量%以上0.8質量%以下であることがより好ましく、0.03質量%以上0.5質量%以下であることが更に好ましい。
The content of glucomannan in the solution containing glucomannan and calcium hydroxide is preferably 0.1% by mass or more and 10% by mass or less, more preferably 0.2% by mass or more and 8% by mass or less, and even more preferably 0.5% by mass or more and 5% by mass or less, based on the entire solution containing glucomannan and calcium hydroxide.
The content of calcium hydroxide in the solution containing glucomannan and calcium hydroxide is preferably 0.01% by mass or more and 1% by mass or less, more preferably 0.02% by mass or more and 0.8% by mass or less, and even more preferably 0.03% by mass or more and 0.5% by mass or less, based on the entire solution containing glucomannan and calcium hydroxide.

 混合液を作製する際に添加される、乳化工程を経て得られる乳化物と、グルコマンナン及び水酸化カルシウムを含む溶液と、の質量比(乳化工程を経て得られる乳化物/グルコ
マンナン及び水酸化カルシウムを含む溶液)は、1/2以上2/1以下であることが好ましい。
The mass ratio of the emulsion obtained through the emulsification process to the solution containing glucomannan and calcium hydroxide added when preparing the mixed liquid (emulsion obtained through the emulsification process/solution containing glucomannan and calcium hydroxide) is preferably 1/2 or more and 2/1 or less.

 乳化工程を経て得られる乳化物と、グルコマンナン及び水酸化カルシウムを含む溶液と、を混合する方法としては、これらの溶液を撹拌機によって混合する方法が挙げられる。
 撹拌機としては、例えば、プロペラ型、アンカー型、パドル型、又はタービン型の撹拌羽根を備えた回転式攪拌機が挙げられる。
As a method for mixing the emulsion obtained through the emulsification step with the solution containing glucomannan and calcium hydroxide, a method in which these solutions are mixed with a stirrer can be mentioned.
Examples of the stirrer include rotary stirrers equipped with propeller-type, anchor-type, paddle-type, or turbine-type stirring blades.

(加熱工程)
 加熱工程は、混合工程を経て得られる混合液を加熱する工程である。
 混合工程を経て得られる混合液は、グルコマンナンを含有する。当該化合物は、アルカリの存在下でゲル化し、グルコマンナンのゲル化物を生成する。加熱工程においては当該反応が主に進行していると推測される。
(Heating process)
The heating step is a step of heating the mixed liquid obtained through the mixing step.
The mixture obtained through the mixing step contains glucomannan. The compound gels in the presence of an alkali to produce a gel of glucomannan. It is presumed that this reaction mainly proceeds during the heating step.

 加熱工程において、混合工程を経て得られる混合液を80℃以上120℃以下に加熱することが好ましい。
 加熱時間としては、5分以上30分以下とすることが好ましい。
In the heating step, the mixed liquid obtained through the mixing step is preferably heated to 80° C. or higher and 120° C. or lower.
The heating time is preferably from 5 minutes to 30 minutes.

 加熱工程は具体的には、混合工程を経て得られる混合液を、沸騰した水の中に加えて加熱する工程が挙げられる。 Specifically, the heating process involves adding the mixture obtained through the mixing process to boiling water and heating it.

 以上の工程を経て脂肪塊組成物を製造することが好ましい。
 なお、以上の工程を経て得られる脂肪塊組成物は、植物性油脂がイオン架橋性ポリマーの多価金属塩及びグルコマンナンのゲル化物で覆われている構造となりやすい。
It is preferable to produce a fat chunk composition through the above steps.
Furthermore, the fat block composition obtained through the above steps tends to have a structure in which the vegetable oil is covered with a polyvalent metal salt of an ionically crosslinkable polymer and a gelled product of glucomannan.

<代替肉の製造方法>
 代替肉の製造方法としては、赤身様部分と、脂肪塊組成物と、を接触させる工程(第1工程)と、結着剤を用いて固定する工程(第2工程)と、を含むことが好ましい。
<Method of producing alternative meat>
The method for producing the alternative meat preferably includes a step (first step) of contacting the red meat-like portion with the fat chunk composition, and a step (second step) of fixing the mixture using a binder.

(赤身様部分の製造方法)
 本開示に係る代替肉の製造方法は、以下の通り行うことが好ましい。
(Method of producing lean meat-like portion)
The method for producing alternative meat according to the present disclosure is preferably carried out as follows.

 赤身様部分を製造する手順の例としては、下記手順が挙げられる。
 手順(1-1)少なくともタンパク質を含有する赤身様部分の原料を押出機から押し出し、押し出された赤身様部分の原料を赤く着色した後、畜肉の赤身肉の形状に似た形状に成型する。
 手順(1-2)市販の大豆肉を赤色の着色剤で着色し、着色した代替肉を畜肉の赤身肉の形状に似た形状に成型する。
 手順(1-3)少なくともタンパク質、及び着色剤を含有する赤身様部分の原料を押出機から押し出し、押し出された赤く着色した赤身様部分を塊肉の赤身肉の形状に似せた形状に成形する。
 手順(1-4)赤く着色した市販の大豆肉を、塊肉の赤身肉の形状に似せた形状に成形する。
 以下に上記手順(1-1)~手順(1-4)の手順について詳細に説明する。
An example of a procedure for producing lean meat-like portions is as follows.
Step (1-1): A lean meat-like raw material containing at least protein is extruded from an extruder, and the extruded lean meat-like raw material is colored red and then molded into a shape similar to that of lean meat of livestock.
Step (1-2): Color commercially available soy meat with a red coloring agent, and mold the colored substitute meat into a shape similar to that of red meat from livestock.
Step (1-3): A raw material of a red meat-like portion containing at least a protein and a colorant is extruded from an extruder, and the extruded red meat-like portion colored red is formed into a shape resembling the shape of a block of red meat.
Step (1-4): Red-colored commercially available soy meat is molded into a shape resembling that of a block of lean meat.
The above steps (1-1) to (1-4) will be described in detail below.

-手順(1-1)-
・赤身肉様部分の原料
 赤身様部分の原料としては、少なくともタンパク質を含有するが、押出機からの赤身様部分の原料の押し出し効率化の観点から、水も含有することが好ましい。
 赤身様部分の原料としては、タンパク質10質量部に対して水を2質量部以上6質量部以上含有することが好ましい。
- Procedure (1-1) -
The lean meat-like portion raw material contains at least protein, but from the viewpoint of efficient extrusion of the lean meat-like portion raw material from the extruder, it is preferable that the lean meat-like portion raw material also contains water.
The lean meat-like portion of the raw material preferably contains 2 to 6 parts by mass of water per 10 parts by mass of protein.

・押出条件
 押出機は、特に限定されず、公知の単軸スクリュー押出機、非噛み合い型異方向回転二軸スクリュー押出機、噛み合い型異方向回転二軸スクリュー押出機、および、噛み合い型同方向回転二軸スクリュー押出機を用いることができる。
Extrusion Conditions The extruder is not particularly limited, and a known single screw extruder, non-intermeshing counter-rotating twin screw extruder, intermeshing counter-rotating twin screw extruder, or intermeshing co-rotating twin screw extruder can be used.

 押出機のバレル温度は、バレル前半部(赤身様部分の原料供給部から、バレル中央までの部分)の温度を60℃以上100℃以下とすることが好ましく、バレル中央(バレルの軸方向長さ中央)の温度を90℃以降170℃以下とすることが好ましく、バレル後半部(バレル中央からバレルの先端までの部分)の温度を140℃以上180℃以下とすることが好ましい。 The extruder barrel temperature is preferably 60°C or higher and 100°C or lower in the front half of the barrel (from the raw material supply section for the lean part to the center of the barrel), 90°C or higher and 170°C or lower in the center of the barrel (center of the axial length of the barrel), and 140°C or higher and 180°C or lower in the rear half of the barrel (from the center of the barrel to the tip of the barrel).

 押出機は、バレルの先端にダイを装着していることが好ましい。
 ダイはシート状の押出物が得られるダイであることが好ましい。
 ダイの吐出口の隙間(リップクリアランス)は1mm以上10mm以下であることが好ましい。吐出口の形状は、平板状でもよく、円筒状でもよい。
 ダイの長さは、30mm以上500mm以下であることが好ましい。
 ダイは冷却ダイであることが好ましい。ここで冷却ダイとは、例えば冷却液(水またはグリコールなど)の循環により冷却されるダイをいう。冷却は、冷却水を循環させることで行ってもよく、空冷することで行ってもよい。
 冷却ダイを用いることで、押し出された赤身様部分の原料の膨化が抑制されやすくなる。そのため、冷却ダイを用いて押し出された赤身様部分の原料を用いて製造した赤身様部分は繊維質となりやすい。
 冷却ダイを用いる場合、冷却ダイの吐出口の温度を90℃以上120℃以下とすることが好ましい。
The extruder preferably has a die attached to the end of the barrel.
The die is preferably one which results in a sheet-like extrudate.
The gap (lip clearance) of the discharge port of the die is preferably 1 mm or more and 10 mm or less. The shape of the discharge port may be flat or cylindrical.
The length of the die is preferably 30 mm or more and 500 mm or less.
The die is preferably a cooling die, which means a die that is cooled, for example, by circulating a cooling liquid (such as water or glycol). The cooling may be performed by circulating cooling water or by air cooling.
The use of a cooling die tends to suppress the expansion of the extruded red meat-like portion raw material, and therefore the red meat-like portion produced using the red meat-like portion raw material extruded using the cooling die tends to be fibrous.
When a cooling die is used, the temperature of the discharge port of the cooling die is preferably set to 90° C. or higher and 120° C. or lower.

・成形
 押し出された赤身様部分の原料は、必要に応じて、切断して使用することが好ましい。
 塊肉に似た外観を有する生肉様代替とする観点からは、例えば、押し出された赤身様部分の原料の押出方向の長さを10mm以上500mmの長さとし、押出方向と直交する方向の長さを2mm以上8mm以下とすることが好ましい。
Molding The extruded lean meat-like portion of the raw material is preferably cut into pieces for use as required.
From the viewpoint of producing a raw meat substitute having an appearance similar to that of a chunk of meat, it is preferable that the length of the extruded red meat-like portion in the extrusion direction of the raw material is 10 mm or more and 500 mm or less, and the length in the direction perpendicular to the extrusion direction is 2 mm or more and 8 mm or less.

 押し出された赤身様部分の原料は、着色剤を用いて赤く着色することが好ましい。
 着色剤としては、可食性かつ赤色の着色剤であることが好ましい。
 着色剤としては、例えば、天然ビーツ赤色色素、コチニール色素、クチナシ赤色素等が挙げられ、中でも、調理時の熱によって退色することから天然ビーツ赤色色素であることが好ましい。
The extruded lean meat raw material is preferably coloured red with a colouring agent.
The coloring agent is preferably an edible red coloring agent.
Examples of coloring agents include natural beetroot red pigment, cochineal pigment, gardenia red pigment, etc., and among these, natural beetroot red pigment is preferred because it fades due to the heat during cooking.

 押し出された赤身様部分の原料に対して、結着剤を添加することが好ましく、必要に応じて調味料を添加してもよい。
 押し出された赤身様部分の原料を塊状に集めて、畜肉の赤身肉の形状に似た形状に成型することで、代替肉の赤身様部分を製造する。
 より畜肉に近い外観を有する代替肉を得る観点から、押し出された赤身様部分の原料を塊状に集める際、押し出された赤身様部分の原料の押出方向をそれぞれ同一に近い方向にそろえることが好ましい。
It is preferable to add a binder to the extruded lean meat-like portion raw material, and a seasoning may be added as necessary.
The extruded raw material of the lean meat-like portion is collected in a block form and molded into a shape similar to that of lean livestock meat, thereby producing the lean meat-like portion of the substitute meat.
From the viewpoint of obtaining a substitute meat having an appearance closer to that of livestock meat, when the extruded lean meat-like portions of the raw material are collected in a block, it is preferable to align the extrusion directions of the extruded lean meat-like portions of the raw material in approximately the same direction.

-手順(1-2)-
 赤身様部分を製造する手順としては、市販の大豆肉を赤色の着色剤で着色し、着色した代替肉を畜肉の赤身肉の形状に似せた形状に成型する手順であってもよい。
 大豆肉とは、大豆由来の植物性タンパク質を含む原料を用いて人工的に製造される食材であり、畜肉に近い食感を有する物を指す。
- Procedure (1-2) -
The procedure for producing the lean meat-like portion may involve coloring commercially available soy meat with a red coloring agent and molding the colored substitute meat into a shape that resembles the shape of lean livestock meat.
Soy meat is a food ingredient artificially produced using ingredients containing vegetable protein derived from soybeans, and has a texture similar to that of animal meat.

 大豆肉は、必要に応じて、切断して使用することが好ましい。
 畜肉に似た外観を有する代替肉とする観点からは、繊維化されている一般的な市販の大豆肉においては、大豆肉の有する筋肉様の組織が有する繊維の束に類似した構造に沿って割くことで調整することが好ましい。例えば、大豆肉の縦幅を10mm以上50mmとし、横幅を2mm以上8mm以下とし、厚さを1mm以上5mm以下の寸法とすることが好ましい。
It is preferable to cut the soybean meat into pieces before use, as needed.
From the viewpoint of obtaining a substitute meat having an appearance similar to that of livestock meat, it is preferable to adjust the size of soy meat by splitting it along a structure similar to the fiber bundles of muscle-like tissues of soy meat, in the case of general commercially available fiberized soy meat. For example, it is preferable to set the length of the soy meat to 10 mm to 50 mm, the width to 2 mm to 8 mm, and the thickness to 1 mm to 5 mm.

 大豆肉は、着色剤を用いて赤く着色することが好ましい。
 着色剤としては、手順(1-1)において挙げたものと同様の着色剤が挙げられる。
 また、大豆肉に対して、結着剤を添加することが好ましく、必要に応じて調味料を添加してもよい。
 塊状に集めて、畜肉の赤身肉の形状に似た形状に成型することで、代替肉の赤身様部分を製造する。
 より畜肉に近い外観を有する代替肉を得る観点から、大豆肉を塊状に集める際、大豆肉の有する筋肉様の組織が有する繊維の束に類似した構造の方向をそれぞれ同一に近い方向にそろえることが好ましい。
The soy meat is preferably coloured red with a colouring agent.
The coloring agent may be the same as those listed in step (1-1).
It is also preferable to add a binder to the soy meat, and seasonings may be added as necessary.
The meat is collected into a lump and molded into a shape similar to that of red meat from livestock, thereby producing the red meat-like portion of the substitute meat.
In order to obtain a substitute meat having an appearance closer to that of livestock meat, when the soy meat is collected into chunks, it is preferable to align the directions of the fiber bundle-like structures of the muscle-like tissue of the soy meat in approximately the same direction.

-手順(1-3)-
 着色剤を押出成形後の赤身様部分の原料に添加することに代えて、押出成形前の赤身様部分の原料に着色剤を添加するようにすること以外は、手順(1-1)と同様に赤身様部分を製造することが好ましい。
- Procedure (1-3) -
It is preferable to produce the red meat-like portion in the same manner as in procedure (1-1), except that instead of adding the colorant to the raw material of the red meat-like portion after extrusion molding, the colorant is added to the raw material of the red meat-like portion before extrusion molding.

-手順(1-4)-
 市販の大豆肉を着色剤で着色することに代えて、あらかじめ赤く着色された大豆肉を用いること以外は、手順(1-2)と同様に赤身様部分を製造することが好ましい。
- Procedure (1-4) -
It is preferable to produce a red meat-like portion in the same manner as in procedure (1-2), except that instead of coloring commercially available soy meat with a coloring agent, soy meat that has already been colored red is used.

(第1工程)
 第1工程は、赤身様部分と、脂肪塊組成物と、を接触させる工程である。
 赤身様部分と、脂肪塊組成物と、を接触させる方法としては、特に限定されないが、例えば、図1の様なステーキ肉に似た外観を有する代替肉を製造する場合、赤身様部分をステーキ肉の赤身に似た形状に成型し、脂肪塊組成物をステーキ肉の脂身に似た形状に成型した後、赤身様部分及び脂肪塊組成物を接触させることが好ましい。
(First step)
The first step is to contact the lean meat-like portion with a fat chunk composition.
The method of contacting the lean meat-like portion with the fat chunk composition is not particularly limited, but for example, when producing a substitute meat having an appearance similar to steak meat as shown in Figure 1, it is preferable to mold the lean meat-like portion into a shape similar to the lean meat of steak meat, mold the fat chunk composition into a shape similar to the fat of steak meat, and then contact the lean meat-like portion with the fat chunk composition.

 ここで、第1工程は、脂肪塊組成物の一部に粉末状の大豆タンパク質を付着し、脂肪塊組成物の粉末状の大豆タンパク質を付着した領域にタンパク質を含む赤身様部分を接着させる工程であることが好ましい。 Here, the first step is preferably a step of attaching powdered soy protein to a portion of the fat lump composition, and adhering a red meat-like portion containing protein to the area of the fat lump composition where the powdered soy protein has been attached.

 具体的には、図1の様なステーキ肉に似た外観を有する代替肉を製造する場合、先ず赤身様部分をステーキ肉の赤身に似た形状に成型し、脂肪塊組成物をステーキ肉の脂身に似た形状に成型する。続いて、脂肪塊組成物のうち、赤身様部分と接触させる領域に粉末状の大豆タンパク質を付着する。その後、粉末状の大豆タンパク質を付着した領域にタンパク質を含む赤身様部分を接着させる工程である。 Specifically, when producing a substitute meat that has an appearance similar to steak meat as shown in Figure 1, first, the lean meat-like portion is molded into a shape similar to the lean meat of steak meat, and the fat chunk composition is molded into a shape similar to the fat of steak meat. Next, powdered soy protein is attached to the area of the fat chunk composition that will come into contact with the lean meat-like portion. This is followed by a process in which the protein-containing lean meat-like portion is adhered to the area where the powdered soy protein has been attached.

 当該工程を含んで製造された代替肉は、赤身様部分と、脂肪塊組成物と、の界面に大豆タンパク質の粉状体を含有する状態となりやすい。そのため、赤身様部分及び脂肪塊組成物の密着性が向上した代替肉が得られやすい。 The meat substitute produced using this process tends to contain soy protein powder at the interface between the red meat-like portion and the fat chunk composition. This makes it easier to obtain meat substitutes with improved adhesion between the red meat-like portion and the fat chunk composition.

(第2工程)
 第2工程は、第1工程で得られた赤身様部分と、脂肪塊組成物と、の接触体を固定する工程である。
 接触体を固定する方法としては、例えば、接触体に一定の圧力を付与した状態で静置する方法が挙げられる。
 ここで、接触体を固定する際、結着剤を接触体全体にまぶした後、静置してもよい。
 ここで結着剤としては、既述のものが適用可能であるが、タンパク質を硬化させる酵素を含むことが好ましく、トランスグルタミナーゼを含むことがより好ましい。
(Second step)
The second step is a step of fixing the contact body of the lean meat-like portion obtained in the first step and the fat chunk composition.
As a method for fixing the contact body, for example, there is a method in which the contact body is left stationary while a certain pressure is applied thereto.
Here, when fixing the contact body, the contact body may be entirely coated with a binder and then allowed to stand.
As the binder, those already mentioned can be used, but it is preferable that the binder contains an enzyme that hardens proteins, and it is more preferable that the binder contains transglutaminase.

 以上の工程を経て代替肉が製造されることが好ましい。 It is preferable that meat substitutes are produced through the above process.

 以下に実施例について説明するが、本開示はこれらの実施例に何ら限定されるものではない。なお、以下の説明において、特に断りのない限り、「%」はすべて質量基準である。 The following examples are provided, but the present disclosure is in no way limited to these examples. In the following description, all percentages are by mass unless otherwise specified.

<実施例1>
 以下の手順で脂肪塊組成物を得た。
(乳化工程)
 イオン架橋性ポリマーとしてアルギン酸ナトリウム(キミカ社製のキミカアルギンI-1)の含有量が溶液全体に対して1質量%であるイオン架橋性ポリマーを含む水溶液を300質量部、及び植物性油脂としてココナッツオイル(アルカピア社輸入販売品、品名:ピアココナ)200質量部を、プロペラ型の撹拌羽根を備えた回転式攪拌機によって、600rpm(revolutions per minutes)で1分間撹拌を行い、乳化物1を得た。
 アルギン酸ナトリウムの1質量%水溶液における粘度(20℃)は、71.1mPa・sであった。
Example 1
A fat mass composition was obtained by the following procedure.
(Emulsification process)
300 parts by mass of an aqueous solution containing an ionically crosslinkable polymer, in which the content of sodium alginate (Kimica Algin I-1 manufactured by Kimica) as the ionically crosslinkable polymer was 1% by mass relative to the entire solution, and 200 parts by mass of coconut oil (imported and sold by Alcapia, product name: Pia Cocona) as the vegetable oil were stirred for 1 minute at 600 rpm (revolutions per minute) using a rotary mixer equipped with a propeller-type stirring blade, to obtain emulsion 1.
The viscosity (20° C.) of a 1% by mass aqueous solution of sodium alginate was 71.1 mPa·s.

(混合溶液)
 グルコマンナンとして、アサヒ食品工業社製のこんにゃく粉(グルコマンナン)50質量部を水1620質量部に溶解させ30分放置した。その後、この溶液に、水酸化カルシウム3質量部を水180質量部に分散させたものを加えよくかき混ぜることでグルコマンナン及び水酸化カルシウムを含むゲル(グルコマンナンのゲル化物)を得た。
 乳化物1に対して、グルコマンナン及び水酸化カルシウムを含むゲル500質量部を加え、撹拌機で撹拌を行い、混合ゲル1を得た。混合ゲル1には、ココナッツオイル及びグルコマンナンのゲルに加え、アルギン酸ナトリウムのカルシウム塩(イオン架橋性ポリマーの多価金属塩)が含まれている。
(Mixed solution)
As glucomannan, 50 parts by mass of konjac flour (glucomannan) manufactured by Asahi Foods Industries Co., Ltd. was dissolved in 1620 parts by mass of water and left for 30 minutes. After that, a dispersion of 3 parts by mass of calcium hydroxide in 180 parts by mass of water was added to this solution and thoroughly stirred to obtain a gel containing glucomannan and calcium hydroxide (gelated glucomannan).
To the emulsion 1, 500 parts by mass of a gel containing glucomannan and calcium hydroxide was added, and the mixture was stirred with a stirrer to obtain a mixed gel 1. The mixed gel 1 contains, in addition to the gel of coconut oil and glucomannan, a calcium salt of sodium alginate (a polyvalent metal salt of an ionically crosslinkable polymer).

(加熱工程)
 混合ゲル1を1時間静置した後、沸騰した水の中に混合ゲル1を加えて5分間加熱した。その後、固形分を取り出すことで、脂肪塊組成物1を得た。
 脂肪塊組成物1は植物性油脂が、アルギン酸多価金属塩及びグルコマンナンのゲル化物で覆われている構造を有していた。
(Heating process)
After leaving the mixed gel 1 for 1 hour, the mixed gel 1 was added to boiling water and heated for 5 minutes. Then, the solid content was taken out to obtain a fat chunk composition 1.
Fat block composition 1 had a structure in which vegetable oil was covered with a gel of polyvalent metal alginate and glucomannan.

<実施例2>
 イオン架橋性ポリマーとして、アルギン酸ナトリウムの替わりに、LMペクチン(ユニクックフーズ社製、品名ミルキーリボン)を用いたこと以外は、実施例1と同様にして脂肪塊組成物2を得た。
 脂肪塊組成物2は植物性油脂が、LMペクチンの多価金属塩及びグルコマンナンのゲル化物で覆われている構造を有していた。
 LMペクチンの1質量%水溶液における粘度(20℃)は、3.13mPa・sであった。
Example 2
Fat block composition 2 was obtained in the same manner as in Example 1, except that LM pectin (manufactured by Unicook Foods, product name Milky Ribbon) was used instead of sodium alginate as the ionically cross-linkable polymer.
Fat block composition 2 had a structure in which vegetable oil was covered with a polyvalent metal salt of LM pectin and a gelled product of glucomannan.
The viscosity (20° C.) of a 1% by mass aqueous solution of LM pectin was 3.13 mPa·s.

<実施例3>
 乳化物1を750質量部に対して、グルコマンナン及び水酸化カルシウムを含むゲル250質量部を加え、撹拌機で撹拌を行い、混合ゲルとしたこと以外は実施例1と同様の手順にて脂肪塊組成物3を得た。
 脂肪塊組成物3は植物性油脂が、アルギン酸多価金属塩及びグルコマンナンのゲル化物で覆われている構造を有していた。
Example 3
Fat block composition 3 was obtained in the same manner as in Example 1, except that 250 parts by mass of a gel containing glucomannan and calcium hydroxide was added to 750 parts by mass of emulsion 1 and stirred with a stirrer to form a mixed gel.
Fat block composition 3 had a structure in which vegetable oil was covered with a gel of polyvalent metal alginate and glucomannan.

<実施例4>
 乳化物1を667質量部に対して、グルコマンナン及び水酸化カルシウムを含むゲル333質量部を加え、撹拌機で撹拌を行い、混合ゲルとしたこと以外は実施例1と同様の手順にて脂肪塊組成物4を得た。
 脂肪塊組成物4は植物性油脂が、アルギン酸多価金属塩及びグルコマンナンのゲル化物で覆われている構造を有していた。
Example 4
Fat block composition 4 was obtained in the same manner as in Example 1, except that 333 parts by mass of a gel containing glucomannan and calcium hydroxide was added to 667 parts by mass of emulsion 1 and stirred with a stirrer to form a mixed gel.
Fat block composition 4 had a structure in which vegetable oil was covered with a gel of polyvalent metal alginate and glucomannan.

<実施例5>
 乳化物1を333質量部に対して、グルコマンナン及び水酸化カルシウムを含むゲル667質量部を加え、撹拌機で撹拌を行い、混合ゲルとしたこと以外は実施例1と同様の手順にて脂肪塊組成物5を得た。
 脂肪塊組成物5は植物性油脂が、アルギン酸多価金属塩及びグルコマンナンのゲル化物で覆われている構造を有していた。
Example 5
Fat block composition 5 was obtained in the same manner as in Example 1, except that 667 parts by mass of a gel containing glucomannan and calcium hydroxide was added to 333 parts by mass of emulsion 1, and stirred with a stirrer to form a mixed gel.
Fat block composition 5 had a structure in which vegetable oil was covered with a gel of polyvalent metal alginate and glucomannan.

<実施例6>
 乳化物1を250質量部に対して、グルコマンナン及び水酸化カルシウムを含むゲル750質量部を加え、撹拌機で撹拌を行い、混合ゲルとしたこと以外は実施例1と同様の手順にて脂肪塊組成物6を得た。
 脂肪塊組成物6は植物性油脂が、アルギン酸多価金属塩及びグルコマンナンのゲル化物で覆われている構造を有していた。
Example 6
Fat block composition 6 was obtained in the same manner as in Example 1, except that 750 parts by mass of a gel containing glucomannan and calcium hydroxide was added to 250 parts by mass of emulsion 1 and stirred with a stirrer to form a mixed gel.
Fat block composition 6 had a structure in which vegetable oil was covered with a gel of polyvalent metal alginate and glucomannan.

<比較例1>
 脱脂大豆(不二製油社製フジプロFR)の含有量が、溶液全体に対して15%であるタンパク質懸濁溶液を75質量部、及び植物性油脂としてココナッツオイル(アルカピア社輸入販売品、品名:ピアココナ)25質量部を、プロペラ型の撹拌羽根を備えた回転式攪拌機によって、600rpmで1分間撹拌を行い、乳化物2を得た。
 乳化物2に対して、架橋剤液(味の素社製スーパーカード10質量%水溶液)を加え、55℃条件下で30分間硬化させ、続いて90℃条件下で30分間酵素の失活処理をすることで、脂肪塊組成物C1を得た。
<Comparative Example 1>
75 parts by mass of a protein suspension having a defatted soybean (Fujipro FR manufactured by Fuji Oil Co., Ltd.) content of 15% relative to the total solution, and 25 parts by mass of coconut oil (imported and sold by Alcapia Co., Ltd., product name: Pia Cocona) as a vegetable oil were stirred at 600 rpm for 1 minute using a rotary mixer equipped with a propeller-type stirring blade, to obtain emulsion 2.
A crosslinker liquid (10% by weight aqueous solution of Supercard manufactured by Ajinomoto Co., Ltd.) was added to the emulsion 2, and the mixture was hardened at 55°C for 30 minutes, followed by an enzyme inactivation treatment at 90°C for 30 minutes to obtain fat block composition C1.

<実施例101>
 以下の手順で代替肉を得た。
<Example 101>
The substitute meat was obtained by the following procedure.

(赤身様部分前駆体の作製)
-赤身様部分の原料の作製-
 タンパク質として脱脂大豆粉(昭和フレッシュRF、昭和産業社製)と、タンパク質として小麦グルテン(PRO-グル65、鳥越製粉社製)と、を7:3(質量比)で混ぜ合わせ、混合粉末1を得た。
 スクリュー長が1100mmでスクリュー先端部の最高温度が155℃になるよう設定した2軸エクストルーダーの吐出部に、長さが350mmの冷却ダイ(ダイ幅:50mm
、リップクリアランス:3mm)を取り付け、冷却ダイの出口温度を105℃で安定化した。250g/minで混合粉末1を該エクストルーダーに導入し、混合粉末1の質量の50質量%の水を該エクストルーダーに加えながら押出機から吐出させ、押出方向に筋肉様の組織を有する(繊維化した)赤身様部分の原料1を得た。
(Preparation of lean meat-like portion precursor)
- Preparation of lean meat-like raw materials -
Defatted soy flour (Showa Fresh RF, Showa Sangyo Co., Ltd.) as a protein and wheat gluten (PRO-Glu 65, Torigoe Flour Milling Co., Ltd.) as a protein were mixed in a mass ratio of 7:3 to obtain mixed powder 1.
The screw length was 1100 mm and the maximum temperature at the tip of the screw was set to 155°C. A cooling die with a length of 350 mm (die width: 50 mm) was attached to the discharge section of the twin-screw extruder.
A cooling die (lip clearance: 3 mm) was attached, and the outlet temperature of the cooling die was stabilized at 105° C. Mixed powder 1 was introduced into the extruder at 250 g/min, and discharged from the extruder while adding water in an amount of 50% by mass of the mixed powder 1 to the extruder, to obtain Raw material 1 of a lean meat-like portion having muscle-like tissue in the extrusion direction (fibrous).

-赤身様部分前駆体の作製-
 赤身様部分の原料1を3L(リットル)の沸騰水で10分間茹で、水気を切った。
 赤身様部分の原料1を300mmの長さに切断し、幅5mm程度になるように繊維方向に沿って裂いた。着色剤としてビーツエキス(三栄源エフエスアイ)を用いて赤く着色し、調味料として塩、胡椒及びナツメグを揉みこみ、短冊状繊維化大豆タンパク1を得た。
 その後、300gの短冊状繊維化大豆タンパク1に結着剤としてスーパーカード(味の素社製トランスグルタミナーゼ)30g及びフジプロFR(不二製油社製大豆粉)30gを添加し、均等になるよう混ぜ合わせ、赤身様部分前駆体Aを得た。
- Preparation of lean meat-like precursors -
The lean meat-like portion of raw material 1 was boiled in 3 L (liters) of boiling water for 10 minutes and then drained.
The red meat-like portion of raw material 1 was cut into a length of 300 mm and torn along the fiber direction to a width of about 5 mm. The raw material was colored red using beet extract (Sanei Gen FSI) as a coloring agent, and salt, pepper, and nutmeg were rubbed in as seasonings to obtain strip-shaped fibrous soy protein 1.
Then, 30 g of Supercard (transglutaminase manufactured by Ajinomoto Co., Ltd.) and 30 g of Fujipro FR (soybean flour manufactured by Fuji Oil Co., Ltd.) were added as binders to 300 g of the strip-shaped fibrous soybean protein 1, and mixed uniformly to obtain a lean meat-like portion precursor A.

(脂肪塊組成物の作製)
 実施例1で得た混合ゲル1を成形し、その一面に脱脂大豆粉(不二製油社製フジプロFR;大豆タンパク質の粉状体)をまぶして表面を軽く練りこみ、1時間静置後、5分間沸騰水に入れ、冷まして脂肪塊組成物101を得た。
(Preparation of fat chunk composition)
The mixed gel 1 obtained in Example 1 was molded, one side of it was sprinkled with defatted soybean flour (Fujipro FR manufactured by Fuji Oil Co., Ltd.; powdered soybean protein) and the surface was lightly kneaded, and the mixture was left to stand for 1 hour, then placed in boiling water for 5 minutes and cooled to obtain fat block composition 101.

(代替肉の作製)
 断面が5mm角の短冊状になるよう切断した脂肪塊組成物1を赤身様部分前駆体Aの一部に混ぜ合わせ、脂肪塊組成物1を混ぜ合わせた赤身様部分前駆体Aを一方向に並べて重ね合わせながら、適切な大きさに切断した脂肪塊組成物101を脱脂大豆粉をまぶした面が、赤身様部分前駆体Aの組織化大豆タンパクを向くように乗せた。このとき、赤身様部分と脂肪塊組成物101との界面に脱脂大豆粉が存在する。次いで、全体がサーロインステーキのブロック肉様になるように形を整え、2時間圧力を加え続けて成形し、ブロック状食肉様食品1を得た。繊維の方向と垂直になるようにブロック状食肉様食品1を25mmの厚みに切断し、ステーキ状霜降り生肉様代替肉1(代替肉)を得た。
 得られた代替肉の写真を図1に示す。
(Production of meat substitutes)
The fat chunk composition 1 cut to a 5 mm square cross section was mixed with a part of the lean meat-like portion precursor A, and the lean meat-like portion precursor A mixed with the fat chunk composition 1 was arranged in one direction and stacked, while the fat chunk composition 101 cut to an appropriate size was placed on top of the lean meat-like portion precursor A so that the surface coated with defatted soybean flour faces the textured soybean protein of the lean meat-like portion precursor A. At this time, the defatted soybean flour is present at the interface between the lean meat-like portion and the fat chunk composition 101. Next, the whole was shaped to resemble a block of sirloin steak, and pressure was applied for 2 hours to form the block of meat-like food 1. The block of meat-like food 1 was cut to a thickness of 25 mm perpendicular to the fiber direction to obtain a steak-like marbled raw meat substitute 1 (substitute meat).
A photograph of the obtained meat substitute is shown in Figure 1.

 <実施例102>
 赤身様部分の原料1に代えて、市販の繊維質の大豆肉(ベジタリアンブッチャー社製、What the cluck)を用いたこと以外は、実施例101と同様の手順にて、赤身様部分前駆体の作製、及び代替肉の作製を行い、ステーキ状霜降り生肉様代替肉2を得た。
<Example 102>
Except for using commercially available fibrous soy meat (Vegetarian Butcher, What the Cluc) instead of the lean meat-like raw material 1, the lean meat-like precursor and the substitute meat were prepared in the same manner as in Example 101, and a steak-shaped marbled raw meat-like substitute meat 2 was obtained.

<実施例103>
 赤身様部分の原料1に代えて、市販のスポンジ質の大豆肉(不二製油社製アペックスー1000)を10分間熱湯で湯戻ししたものを用いたこと以外は、実施例101と同様の手順にて、赤身様部分前駆体の作製、及び代替肉の作製を行い、ステーキ状霜降り生肉様代替肉3を得た。
<Example 103>
The same procedures as in Example 101 were used to prepare a lean meat precursor and a substitute meat, except that instead of the lean meat-like raw material 1, commercially available spongy soy meat (Apex-1000 manufactured by Fuji Oil Co., Ltd.) that had been rehydrated in hot water for 10 minutes was used, and a steak-like marbled raw meat-like substitute meat 3 was obtained.

<比較例101>
 <実施例101>における(脂肪塊組成物の作製)を行わず、(代替肉の作製)の手順を下記の通りに変更したこと以外は実施例101と同一の手順にて、赤身様部分前駆体の作製、及び代替肉の作製を行い、ステーキ状霜降り生肉様代替肉C1を得た。
<Comparative Example 101>
Except for not performing (preparation of fat block composition) in <Example 101> and changing the procedure for (preparation of substitute meat) as follows, the same procedure as in Example 101 was followed to prepare a lean meat-like portion precursor and a substitute meat, thereby obtaining a steak-like marbled raw meat-like substitute meat C1.

(代替肉の作製)
 断面が5mm角の短冊状になるよう切断した脂肪塊組成物C1を赤身様部分前駆体Aの一部に混ぜ合わせ、脂肪塊組成物C1を混ぜ合わせた赤身様部分前駆体Aを一方向に並べ
て重ね合わせた。次いで、全体がサーロインステーキのブロック肉様になるように形を整え、2時間圧力を加え続けて成形し、ブロック状食肉様食品C1を得た。繊維の方向と垂直になるようにブロック状食肉様食品1を25mmの厚みに切断し、ステーキ状霜降り生肉様代替肉C1(代替肉)を得た。
(Production of meat substitutes)
The fat chunk composition C1 cut into a 5 mm square strip was mixed with a part of the lean meat-like portion precursor A, and the lean meat-like portion precursor A mixed with the fat chunk composition C1 was arranged in one direction and stacked. The whole was then shaped to resemble a block of sirloin steak, and pressure was applied for 2 hours to form the block of meat-like food C1. The block of meat-like food 1 was cut into a thickness of 25 mm perpendicular to the fiber direction to obtain a steak-like marbled raw meat substitute C1 (substitute meat).

<試験・評価>
 各例で得られた脂肪塊組成物、及びステーキ状霜降り生肉様代替肉をホットプレートで加熱調理を行った。加熱調理後の脂肪塊組成物及びステーキ状霜降り生肉様代替肉について以下の評価を行った。
<Testing and Evaluation>
The fat block composition and steak-shaped marbled raw meat substitute obtained in each example were cooked on a hot plate. The fat block composition and steak-shaped marbled raw meat substitute after cooking were evaluated as follows.

(多重バイト試験)
 既述の方法で多重バイト試験におけるToughnessを測定した。
(Multibyte test)
The toughness in the multiple bite test was measured by the method already described.

(外観評価)
 加熱調理を行った後の脂肪塊組成物、又はステーキ状霜降り生肉様代替肉に含まれる脂肪様部分を10人のパネラーが目視評価し、加熱された畜肉由来の脂身に似た外観を有するか否かを評価し、人数の集計を行った。
 肯定的な回答を行った人数を基に下記評価基準により評価した。
-評価基準-
A:肯定的な回答を行った人が7人以上
B:肯定的な回答を行った人が5人以上6人以下
C:肯定的な回答を行った人が4人以下
(Appearance Evaluation)
Ten panelists visually evaluated the fat-like portions contained in the fat chunk composition or steak-like marbled raw meat substitute meat after cooking, evaluating whether they had an appearance similar to fat derived from cooked livestock meat, and the number of people who evaluated them was then tallied.
The number of people who answered affirmatively was used to evaluate the results according to the following criteria.
-Evaluation criteria-
A: 7 or more people answered affirmatively. B: 5 to 6 people answered affirmatively. C: 4 or less people answered affirmatively.

(食感評価)
 加熱調理を行った後の脂肪塊組成物、又はステーキ状霜降り生肉様代替肉を、10人のパネラーが食し、加熱された畜肉由来の脂身の弾力に似た食感及び咀嚼時に油が流出する油感を有するか否かを評価し、人数の集計を行った。
 肯定的な回答を行った人数を基に評価した。なお、評価基準は外観評価と同一とした。
(Texture evaluation)
Ten panelists ate the fat block composition after cooking or the steak-like marbled raw meat substitute, and evaluated whether it had a texture similar to the elasticity of fat derived from cooked meat and an oily feel in which oil flowed out when chewing, and the number of people who ate it was then tallied.
The evaluation was based on the number of people who answered affirmatively. The evaluation criteria were the same as those for the appearance evaluation.

(切断時の密着性評価)
 加熱調理を行った後の代替肉を、10人のパネラーがナイフで切断を行った。この時、切断面が赤身様部分及び脂肪様部分の界面を含むようにした。切断後の代替肉において、赤身様部分からの脂肪様部分の剥がれが生じたか否かについて評価し、人数の集計を行った。
 肯定的な回答(剥がれが生じなかったと回答)を行った人数を基に評価した。なお、評価基準は外観評価と同一とした。
(Adhesion evaluation when cut)
Ten panelists cut the alternative meat after cooking with a knife. At this time, the cut surface included the interface between the lean meat-like portion and the fat-like portion. After cutting, the alternative meat was evaluated for whether or not the fat-like portion peeled off from the lean meat-like portion, and the number of people who cut the alternative meat was counted.
The evaluation was based on the number of people who answered affirmatively (answered that peeling did not occur). The evaluation criteria were the same as those for the appearance evaluation.


 

 

 表中の記載について説明する。
 「多価金属イオン種」は、イオン架橋性ポリマーの多価金属塩に含有される多価金属イオンを示す。
 「牛脂身」は、牛肉の脂身を用いたことを示す。なお、多重バイト試験のみ評価を行った。
 「豚脂身」は、豚肉の脂身を用いたことを示す。なお、多重バイト試験のみ評価を行った。
 「界面での粉状体の有無」は、赤身様部分と、脂肪塊組成物と、の界面に大豆タンパク質の分状態を有するか否かを示す。粉状体を有する場合「有」と記載し、粉状体を有しない場合「-」と記載する。
The contents of the table will be explained below.
The "polyvalent metal ion species" refers to the polyvalent metal ion contained in the polyvalent metal salt of the ionically crosslinkable polymer.
"Beef fat" indicates that beef fat was used. Note that only the multiple bite test was performed.
"Pork fat" indicates that pork fat was used. Note that only the multiple bite test was performed.
"Presence or absence of powdery body at the interface" indicates whether or not there is a soy protein fraction at the interface between the red meat-like portion and the fat chunk composition. If there is a powdery body, it is recorded as "present", and if there is no powdery body, it is recorded as "-".

 上記結果から、本実施例の脂肪塊組成物及び代替肉は、畜肉に含まれる脂身に似た食感を有すること、及び畜肉に含まれる脂身に似た食感を有する脂肪塊組成物を含むことがわかる。 The above results show that the fat chunk composition and meat substitute of this example have a texture similar to the fat contained in livestock meat, and contain a fat chunk composition that has a texture similar to the fat contained in livestock meat.

 2023年3月1日に出願された日本国特許出願2023-031435号の開示は、その全体が参照により本明細書に取り込まれる。また、本明細書に記載された全ての文献、特許出願および技術規格は、個々の文献、特許出願、及び技術規格が参照により取り込まれることが具体的かつ個々に記された場合と同程度に、本明細書中に参照により取り込まれる。 The disclosure of Japanese Patent Application No. 2023-031435, filed on March 1, 2023, is incorporated herein by reference in its entirety. In addition, all documents, patent applications, and technical standards described herein are incorporated herein by reference to the same extent as if each individual document, patent application, and technical standard was specifically and individually indicated to be incorporated by reference.

Claims (9)

 植物性油脂と、
 イオン架橋性ポリマーの多価金属塩と、
 グルコマンナンのゲル化物と、を含有する脂肪塊組成物。
Vegetable oils and fats,
A polyvalent metal salt of an ionically crosslinkable polymer;
A fat mass composition comprising a gelled product of glucomannan.
 前記イオン架橋性ポリマーがアルギン酸又はLMペクチンである請求項1に記載の脂肪塊組成物。 The fat mass composition according to claim 1, wherein the ionically cross-linkable polymer is alginic acid or LM pectin.  前記植物性油脂が、前記アルギン酸又はLMペクチンの多価金属塩及び前記グルコマンナンのゲル化物で覆われている請求項2に記載の脂肪塊組成物。 The fat block composition according to claim 2, wherein the vegetable oil is covered with the polyvalent metal salt of alginic acid or LM pectin and the gel of glucomannan.  多重バイト試験におけるToughnessが1000gw・cm/cm以上10000gw・cm/cm以下である請求項1~請求項3のいずれか1項に記載の脂肪塊組成物。 A fat block composition according to any one of claims 1 to 3, having a toughness in a multiple bite test of 1000 gw·cm/ cm2 or more and 10000 gw·cm/ cm2 or less.  タンパク質を含む赤身様部分と、
 請求項1に記載の脂肪塊組成物と、
を有する代替肉。
A red meat-like portion containing protein;
The fat mass composition according to claim 1 ,
A meat substitute having the above properties.
 前記赤身様部分と、前記脂肪塊組成物と、の界面に大豆タンパク質の粉状体を含有する請求項5に記載の代替肉。 The meat substitute according to claim 5, which contains soy protein powder at the interface between the red meat-like portion and the fat mass composition.  前記赤身様部分がスポンジ質又は繊維質である請求項5又は請求項6に記載の代替肉。 The meat substitute according to claim 5 or 6, wherein the red meat-like portion is spongy or fibrous.  植物性油脂と、イオン架橋性ポリマーを含む水溶液と、を乳化して乳化物を得る工程と、
 前記乳化物と、グルコマンナン及び水酸化カルシウムを含む溶液と、を混合して混合液を得る工程と、
 前記混合液を加熱する工程と、を含む脂肪塊組成物の製造方法。
A step of emulsifying a vegetable oil and an aqueous solution containing an ionically crosslinkable polymer to obtain an emulsion;
A step of mixing the emulsion with a solution containing glucomannan and calcium hydroxide to obtain a mixed liquid;
A method for producing a fat chunk composition comprising the steps of: heating the mixture.
 請求項8に記載の製造方法で得られた脂肪塊組成物の一部に粉末状の大豆タンパク質を付着し、前記脂肪塊組成物の前記粉末状の大豆タンパク質を付着した領域にタンパク質を含む赤身様部分を接触させる工程を含む請求項5に記載の代替肉の製造方法。 The method for producing the meat substitute according to claim 5, comprising the steps of attaching powdered soy protein to a portion of the fat chunk composition obtained by the method according to claim 8, and contacting a red meat-like portion containing protein with the area of the fat chunk composition to which the powdered soy protein has been attached.
PCT/JP2024/004147 2023-03-01 2024-02-07 Fat mass composition, meat substitute, method for producing fat mass composition, and method for producing meat substitute Pending WO2024181072A1 (en)

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JP2010200627A (en) * 2009-02-27 2010-09-16 House Foods Corp Pseudo meat food and method for producing the same
CN102845764A (en) * 2012-09-04 2013-01-02 湖南唐人神西式肉制品有限公司 Low fat Chinese sausage and production method thereof
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Publication number Priority date Publication date Assignee Title
WO2007013146A1 (en) * 2005-07-27 2007-02-01 Stc System Japan Co., Ltd Composition for processed soy protein food, pate for processed food with or without meat, and food resembling dried meat
JP2010200627A (en) * 2009-02-27 2010-09-16 House Foods Corp Pseudo meat food and method for producing the same
CN102845764A (en) * 2012-09-04 2013-01-02 湖南唐人神西式肉制品有限公司 Low fat Chinese sausage and production method thereof
JP2018130102A (en) * 2017-02-17 2018-08-23 Jbridge株式会社 Method for producing vegetarian and vegan substitute meat
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