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WO2024173380A3 - Method for acquiring cellular spatial information using light-sensitive dna barcodes - Google Patents

Method for acquiring cellular spatial information using light-sensitive dna barcodes Download PDF

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Publication number
WO2024173380A3
WO2024173380A3 PCT/US2024/015575 US2024015575W WO2024173380A3 WO 2024173380 A3 WO2024173380 A3 WO 2024173380A3 US 2024015575 W US2024015575 W US 2024015575W WO 2024173380 A3 WO2024173380 A3 WO 2024173380A3
Authority
WO
WIPO (PCT)
Prior art keywords
photocleavable
oligonucleotides
light
spatial information
dna barcodes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2024/015575
Other languages
French (fr)
Other versions
WO2024173380A2 (en
Inventor
Robert PISCOPIO
Alex CHIALASTRI
Max Wilson
Siddharth DEY
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Publication of WO2024173380A2 publication Critical patent/WO2024173380A2/en
Publication of WO2024173380A3 publication Critical patent/WO2024173380A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Many embodiments described herein employ photocleavable oligonucleotides that can be used to encode cells for spatial locality within a field, which can be assessed during downstream nucleic acid analysis (e.g., sequencing). Such oligonucleotides can be referred to as Photocleavable Hashtag Oligos (PHOs). Further embodiments attach these photocleavable oligonucleotides to a cell membrane. Such attachment can include permanent labelling of cells, such as through covalent bonding, strong antibody interactions or strong biotin-streptavidin linkage, or less permanent labelling through the use of cholesterol anchors.
PCT/US2024/015575 2023-02-13 2024-02-13 Method for acquiring cellular spatial information using light-sensitive dna barcodes Ceased WO2024173380A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363484701P 2023-02-13 2023-02-13
US63/484,701 2023-02-13

Publications (2)

Publication Number Publication Date
WO2024173380A2 WO2024173380A2 (en) 2024-08-22
WO2024173380A3 true WO2024173380A3 (en) 2024-12-19

Family

ID=92420653

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/015575 Ceased WO2024173380A2 (en) 2023-02-13 2024-02-13 Method for acquiring cellular spatial information using light-sensitive dna barcodes

Country Status (1)

Country Link
WO (1) WO2024173380A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190316182A1 (en) * 2016-12-23 2019-10-17 Cs Genetics Limited Reagents and methods for molecular barcoding of nucleic acids of single cells
US20220403452A1 (en) * 2019-09-06 2022-12-22 10X Genomics, Inc. Systems and methods for barcoding cells and cell beads

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190316182A1 (en) * 2016-12-23 2019-10-17 Cs Genetics Limited Reagents and methods for molecular barcoding of nucleic acids of single cells
US20220403452A1 (en) * 2019-09-06 2022-12-22 10X Genomics, Inc. Systems and methods for barcoding cells and cell beads

Also Published As

Publication number Publication date
WO2024173380A2 (en) 2024-08-22

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