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WO2024163450A3 - Methods and systems for polymer synthesis by contacting synthesis surfaces with compartmentalized liquid reagents - Google Patents

Methods and systems for polymer synthesis by contacting synthesis surfaces with compartmentalized liquid reagents Download PDF

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Publication number
WO2024163450A3
WO2024163450A3 PCT/US2024/013513 US2024013513W WO2024163450A3 WO 2024163450 A3 WO2024163450 A3 WO 2024163450A3 US 2024013513 W US2024013513 W US 2024013513W WO 2024163450 A3 WO2024163450 A3 WO 2024163450A3
Authority
WO
WIPO (PCT)
Prior art keywords
synthesis
methods
systems
nucleotides
protected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2024/013513
Other languages
French (fr)
Other versions
WO2024163450A2 (en
Inventor
Tuan Tran
Evan Foster
Joseph KOSCINSKI, Jr.
Christopher HOFF
Margarita KHARITON
Julia Swavola
Sebastian PALLUK
Urmi BHAUMIK
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ansa Biotechnologies Inc
Original Assignee
Ansa Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ansa Biotechnologies Inc filed Critical Ansa Biotechnologies Inc
Publication of WO2024163450A2 publication Critical patent/WO2024163450A2/en
Publication of WO2024163450A3 publication Critical patent/WO2024163450A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Structural Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Disclosed herein are methods and devices for improved de novo cyclic enzymatic polymer synthesis using a protected monomer. Protected monomers include those with a protecting group that prevents addition of more than one monomer during a synthesis step, including the catalytic enzyme attached to the monomer. In specific embodiments, the polymer synthesis method is an enzymatic de novo polynucleotide synthesis method using protected nucleotides, such as nucleotides comprising a reversible terminator or nucleotides bound to a catalytic polymerase.
PCT/US2024/013513 2023-01-30 2024-01-30 Methods and systems for polymer synthesis by contacting synthesis surfaces with compartmentalized liquid reagents Ceased WO2024163450A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363441969P 2023-01-30 2023-01-30
US63/441,969 2023-01-30

Publications (2)

Publication Number Publication Date
WO2024163450A2 WO2024163450A2 (en) 2024-08-08
WO2024163450A3 true WO2024163450A3 (en) 2024-09-19

Family

ID=92147548

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/013513 Ceased WO2024163450A2 (en) 2023-01-30 2024-01-30 Methods and systems for polymer synthesis by contacting synthesis surfaces with compartmentalized liquid reagents

Country Status (1)

Country Link
WO (1) WO2024163450A2 (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5592289A (en) * 1995-01-09 1997-01-07 Molecular Dynamics Self-aligning mechanism for positioning analyte receptacles
US5650327A (en) * 1990-03-02 1997-07-22 Ventana Medical Systems, Inc. Method for mixing reagent and sample mounted on a slide
US20040234970A1 (en) * 2001-01-27 2004-11-25 Yoo Jae Chern Nucleic hybridization assay method and device using a cleavage technique responsive to the complementary double strand or the single strand of nucleic acids or oligonucleotides
US6831994B2 (en) * 1997-05-23 2004-12-14 Lynx Therapeutics, Inc. System and apparatus for sequential processing of analytes
US20180094316A1 (en) * 2000-02-07 2018-04-05 Illumina, Inc. Multiplex nucleic acid reactions
US20190352327A1 (en) * 2018-05-15 2019-11-21 Illumina, Inc. Compositions and methods for chemical cleavage and deprotection of surface-bound oligonucleotides
WO2023115040A2 (en) * 2021-12-16 2023-06-22 Ansa Biotechnologies, Inc. Methods of polynucleotide synthesis

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5650327A (en) * 1990-03-02 1997-07-22 Ventana Medical Systems, Inc. Method for mixing reagent and sample mounted on a slide
US5592289A (en) * 1995-01-09 1997-01-07 Molecular Dynamics Self-aligning mechanism for positioning analyte receptacles
US6831994B2 (en) * 1997-05-23 2004-12-14 Lynx Therapeutics, Inc. System and apparatus for sequential processing of analytes
US20180094316A1 (en) * 2000-02-07 2018-04-05 Illumina, Inc. Multiplex nucleic acid reactions
US20040234970A1 (en) * 2001-01-27 2004-11-25 Yoo Jae Chern Nucleic hybridization assay method and device using a cleavage technique responsive to the complementary double strand or the single strand of nucleic acids or oligonucleotides
US20190352327A1 (en) * 2018-05-15 2019-11-21 Illumina, Inc. Compositions and methods for chemical cleavage and deprotection of surface-bound oligonucleotides
WO2023115040A2 (en) * 2021-12-16 2023-06-22 Ansa Biotechnologies, Inc. Methods of polynucleotide synthesis

Also Published As

Publication number Publication date
WO2024163450A2 (en) 2024-08-08

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