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WO2024159977A1 - Pansement dégradable ayant des effets antibactériens doubles, son procédé de préparation et son utilisation - Google Patents

Pansement dégradable ayant des effets antibactériens doubles, son procédé de préparation et son utilisation Download PDF

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Publication number
WO2024159977A1
WO2024159977A1 PCT/CN2023/141678 CN2023141678W WO2024159977A1 WO 2024159977 A1 WO2024159977 A1 WO 2024159977A1 CN 2023141678 W CN2023141678 W CN 2023141678W WO 2024159977 A1 WO2024159977 A1 WO 2024159977A1
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Prior art keywords
dressing
degradable
wound dressing
preparing
film
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Ceased
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PCT/CN2023/141678
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English (en)
Chinese (zh)
Inventor
王怀雨
梁嘉琪
童丽萍
高昂
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Shenzhen Institute of Advanced Technology of CAS
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Shenzhen Institute of Advanced Technology of CAS
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/26—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A61L15/62—Compostable, hydrosoluble or hydrodegradable materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • the present invention relates to the technical field of biomedical materials, and in particular to a degradable wound dressing with dual antibacterial effects, a preparation method thereof and an application thereof.
  • Wound healing is a sequential multi-level process, including hemostasis and inflammation, proliferation and remodeling.
  • wound infection caused by bacteria is one of the main reasons for delayed wound healing.
  • the surface environment of the wound site exposed to the external environment is generally relatively moist, providing a favorable environment for bacterial growth in terms of moisture, temperature and nutrition.
  • the colonization and growth of bacteria in the wound site can cause the wound to enter an inflammatory state, the wound site expands and becomes hypoxic, and the blood vessels are occluded. If there is no effective treatment, the wound will become a chronic healing wound. Severe bacterial colonization and infection will lead to tissue death in the local wound site and even endanger life. Therefore, debridement surgery is often required to clean up necrotic tissue after wound infection.
  • the present invention provides a degradable wound dressing with dual antibacterial efficacy, and a preparation method and application thereof.
  • a first aspect of the present invention provides a method for preparing a degradable wound dressing with dual antibacterial efficacy, the method comprising: firstly incorporating a substance with photothermal conversion performance into a dressing substrate, and then preparing a dressing film loaded with a photothermal agent by a solvent volatilization method; then treating the surface of the dressing film by plasma to form active groups on the surface; and incubating the plasma-treated dressing film in a solution containing antimicrobial peptides to covalently graft the antimicrobial peptides to the surface of the dressing film through the active groups formed on the surface, thereby obtaining a degradable wound dressing with dual antimicrobial efficacy.
  • the step of first incorporating a substance having photothermal conversion properties into a dressing base material and then obtaining a dressing film loaded with a photothermal agent by a solvent volatilization method includes: adding a photothermal agent having a photothermal conversion effect into a volatile organic solvent, then adding the organic solvent to a mold, and after the organic solvent evaporates, transferring the mold to a vacuum drying oven, and after the organic solvent completely evaporates, obtaining a dressing film loaded with a photothermal agent.
  • the dressing substrate is a degradable polymer; wherein the degradable polymer is one or more copolymers of polylactic acid (PLA), polycaprolactone (PCL), polylactic acid polyglycolic acid (PLGA), aliphatic polyester polymers, and aromatic-aliphatic copolyesters, and the molecular weight of the degradable polymer is 50,000 Daltons to 200,000 Daltons.
  • the degradable polymer is one or more copolymers of polylactic acid (PLA), polycaprolactone (PCL), polylactic acid polyglycolic acid (PLGA), aliphatic polyester polymers, and aromatic-aliphatic copolyesters, and the molecular weight of the degradable polymer is 50,000 Daltons to 200,000 Daltons.
  • the process parameters of the plasma treatment include: power of 30 W to 300 W, gas pressure of 0.2 mbar to 1.0 mbar, and treatment time of 5 min to 120 min.
  • the gas used for the plasma treatment is one or more of argon, nitrogen, oxygen, hydrogen, and ammonia.
  • the grafting time for grafting the antimicrobial peptides to the surface of the dressing film is 6 h to 72 h.
  • the antimicrobial peptides used in the solution containing antimicrobial peptides are one or more of HHC36 antimicrobial peptide, LL-37 antimicrobial peptide, and GL13K antimicrobial peptide; wherein the polypeptide sequence of the HHC36 antimicrobial peptide contains KRWWKWWRR; the polypeptide sequence of the LL-37 antimicrobial peptide contains LLGFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES; and the polypeptide sequence of the GL13K antimicrobial peptide contains GKIIKLKASLKLL.
  • the solution concentration of the antimicrobial peptide-containing solution is 100 ⁇ M/mL - 10000 ⁇ M/mL.
  • the second aspect of the present invention provides a degradable wound dressing with dual antibacterial efficacy prepared by the above-mentioned method for preparing a degradable wound dressing with dual antibacterial efficacy.
  • the third aspect of the present invention provides the use of the above-mentioned degradable wound dressing with dual antibacterial efficacy in the medical field.
  • the prepared wound dressing has a dual antibacterial effect.
  • the antibacterial effect of the dressing comes from the antibacterial peptide on the one hand, and from the local thermal effect generated by the photothermal agent loaded inside the dressing under near-infrared light irradiation on the other hand. Therefore, compared with the dressing with a single antibacterial effect, the wound dressing prepared by the present invention has a more excellent anti-infection performance;
  • the present invention requires less antimicrobial peptide loading, and the local thermal effect required to achieve the same antibacterial effect is also lower, thereby avoiding toxicity and damage to tissue cells;
  • the prepared wound dressing has better biosafety.
  • the dressing substrate is made of degradable polymer materials and the degradation products have no toxic side effects; the thermal effect produced by the photothermal agent loaded in the dressing can be regulated by the irradiation intensity of near-infrared light, and will not affect tissue cells under appropriate parameters; the antimicrobial peptide grafted on the surface of the dressing has excellent biosafety and will not produce biological toxicity;
  • the preparation method is simple, efficient and low-cost, suitable for large-scale industrial production.
  • FIG1 is a macroscopic image and a scanning electron microscope surface morphology image of PLGA and BP@PLGA samples in Example 1;
  • FIG2 is a macroscopic image and a scanning electron microscope surface morphology image of the BP@PLGA+P sample in Example 2;
  • FIG3 is a macroscopic image and a scanning electron microscope surface morphology image of the BP@PLGA+A sample in Example 3;
  • FIG4 is the contact angles of PLGA, BP@PLGA, BP@PLGA+P and BP@PLGA+A samples in Example 4;
  • FIG5a is a thermal imaging diagram of the change in surface temperature of the BP@PLGA sample during near-infrared light irradiation for 10 min in Example 5;
  • Figure 5b is the variation trend of the surface temperature of PLGA, BP@PLGA and BP@PLGA+A samples in Example 5 during near-infrared light irradiation for 10 min;
  • FIG6 is a macroscopic photograph of PLGA and BP@PLGA in Example 6 during immersion in phosphate buffer for 14 days;
  • Figure 7a shows the coating conditions of BP@PLGA, BP@PLGA+A and BP@PLGA+A+L flat plates in Example 7;
  • FIG7b is the antibacterial rate of BP@PLGA, BP@PLGA+A and BP@PLGA+A+L samples in Example 7;
  • Figure 8 is the cck-8 results of mouse fibroblast L929 cells cultured on the surfaces of PLGA, BP@PLGA, BP@PLGA+A, and BP@PLGA+A+L samples in Example 8 after 1, 3, and 5 days;
  • FIG9 is a flow chart of a method for preparing a degradable wound dressing with dual antibacterial efficacy provided by the present invention.
  • the inventors have found that by directly or indirectly incorporating some common antibiotics, such as vancomycin, ciprofloxacin, amoxicillin and gentamicin, into dressings, a dressing with antibacterial properties can be obtained.
  • some common antibiotics such as vancomycin, ciprofloxacin, amoxicillin and gentamicin
  • incorporating some metal ions with excellent antibacterial activity such as silver, zinc, copper ions, etc.
  • incorporating some metal ions with excellent antibacterial activity such as silver, zinc, copper ions, etc.
  • the release of these metal ions is difficult to control, and at high concentrations, they are prone to potential cytotoxicity, inflammatory reactions, genetic toxicity and other adverse effects. Therefore, how to prepare a wound dressing that not only has excellent biosafety but also has more efficient antibacterial properties remains a technical problem.
  • the present invention provides a degradable wound dressing with dual antibacterial effects.
  • the dressing uses antimicrobial peptides covalently grafted on the surface to achieve antibacterial properties.
  • Antimicrobial peptides are cationic amphiphilic compounds whose surface contains high-density positive charges and a large number of hydrophobic amino acids, so they can interact electrostatically with the surface of bacteria or biofilms, thereby achieving antibacterial effects.
  • antimicrobial peptides Compared with antibiotics, antimicrobial peptides have the advantages of broad-spectrum and rapid bactericidal activity, inhibiting the formation of bacterial biofilms, and regulating host inflammatory responses, which makes antimicrobial peptides an ideal candidate drug for treating drug-resistant strains.
  • the dressing uses the thermal effect generated by the loaded photothermal agent under near-infrared light irradiation to further improve the antibacterial properties of the dressing.
  • the photothermal agent loaded in the dressing converts light energy into thermal energy, causing local heat to rise and diffuse to the surroundings, forming a high-temperature microenvironment.
  • This high temperature environment can destroy the structural integrity of bacterial cell walls and cell membranes, causing the outflow of important intracellular substances and the destruction of the internal structure of bacteria; at the same time, it causes protein and enzyme denaturation and even DNA damage, interferes with cell metabolic activities, and causes irreversible damage to bacteria, thereby achieving the effect of photothermal sterilization.
  • the thermal effect When the thermal effect is used alone for sterilization, a very high temperature is required.
  • the local temperature required to achieve the same antibacterial effect is lower, thereby avoiding the damage of local high temperature to human tissue cells.
  • the present invention provides a degradable wound dressing with excellent biosafety and dual antibacterial effects.
  • the first aspect of the present invention provides a method for preparing a degradable wound dressing with dual antibacterial efficacy, the method comprising: firstly incorporating a substance with photothermal conversion performance into a dressing substrate, and then preparing a dressing film loaded with a photothermal agent by a solvent volatilization method; then treating the surface of the dressing film by plasma, thereby forming active groups on its surface; and incubating the plasma-treated dressing film in a solution containing antimicrobial peptides, so as to covalently graft the antimicrobial peptides to the surface of the dressing film through the active groups formed on the surface, thereby obtaining a degradable wound dressing with dual antimicrobial efficacy.
  • the wound dressing achieves a dual antibacterial effect by, on the one hand, generating a local thermal effect of the internally loaded photothermal agent under near-infrared light irradiation, and, on the other hand, by the antimicrobial peptides grafted on the surface. And under the superposition of the dual antibacterial effects, the local thermal effect required to achieve the same antibacterial effect is lower, thereby avoiding the damage of local high temperature to tissue cells.
  • the second aspect of the present invention provides a degradable wound dressing with dual antibacterial efficacy prepared by the above-mentioned method for preparing a degradable wound dressing with dual antibacterial efficacy.
  • the third aspect of the present invention provides the use of the above-mentioned degradable wound dressing with dual antibacterial efficacy in the medical field.
  • the biodegradable polymer material polylactic acid-co-glycolic acid (PLGA) was selected as the base material of the dressing, black phosphorus nanosheets were used as the photothermal agent, and the HHC36 antimicrobial peptide was covalently grafted onto the surface of the dressing to demonstrate the feasibility of the present invention.
  • PLGA polylactic acid-co-glycolic acid
  • PLGA poly(lactic-co-glycolic acid)
  • BP black phosphorus nanosheets
  • 375 ⁇ g of black phosphorus nanosheets were redispersed in the chloroform solution of PLGA, and ultrasonication was performed for 10 min to evenly disperse black phosphorus in the mixture to obtain a PLGA/BP mixture.
  • a circular cover glass with a diameter of 14 mm was used as a mold.
  • the cover glass was ultrasonically cleaned with alcohol and then blown dry for later use.
  • 250 ⁇ L of the above PLGA/BP mixture was dripped on the surface of the disc, and then placed in a sealed container at room temperature for 24 h before being transferred to a vacuum drying oven.
  • the solvent was fully evaporated under a vacuum environment to remove the residual organic solvent.
  • a PLGA dressing wrapped with black phosphorus was obtained by film formation, which was named BP@PLGA.
  • a PLGA dressing was prepared using a PLGA solution without black phosphorus doping by the same solvent evaporation method.
  • the sample was named PLGA.
  • the macroscopic and surface microscopic morphologies of the samples were observed using a digital camera and a scanning electron microscope.
  • the appearance of the prepared PLGA film is colorless and transparent, and it is a dense film.
  • the appearance of the PLGA film wrapped with black phosphorus is a light brown film with a smooth and flat surface.
  • a small amount of black phosphorus nanosheets exposed on the surface can be observed by observing the surface morphology through an electron microscope, indicating that black phosphorus is successfully loaded in the PLGA film.
  • the BP@PLGA prepared in Example 1 was treated with nitrogen plasma.
  • the sample was placed in the sample chamber of the plasma treatment machine, evacuated, and then the RF power was set to 300 W. Argon was introduced to make the pressure in the sample chamber reach about 0.4 mbar, and the plasma treatment was activated for 10 minutes. The sample was taken out after the treatment.
  • the treated sample was named BP@PLGA+P.
  • a digital camera and a scanning electron microscope were used to observe the macroscopic and surface microscopic morphology of the sample after plasma treatment.
  • Example 2 The sample (BP@PLGA+P) in Example 2 was immersed in a sterile aqueous solution containing 500 ⁇ M antimicrobial peptide HHC36 (the polypeptide sequence contains KRWWKWWRR) and stored at 4°C for 24 hours. The sample was then taken out of the antimicrobial peptide solution and rinsed three times with sterile water to remove the ungrafted antimicrobial peptide. The sample was labeled as BP@PLGA+A. The macroscopic and surface microscopic morphology of the sample after grafting the antimicrobial peptide was observed using a digital camera and a scanning electron microscope. As shown in Figure 3, compared with the sample without grafted antimicrobial peptide, the grafted antimicrobial peptide had no significant effect on the macroscopic and microscopic morphology of the material surface.
  • the hydrophilicity of the BP@PLGA+P sample was significantly improved, and the contact angle was greatly reduced to 12°.
  • the contact angle of the sample BP@PLGA+A increased to 30°, which indirectly indicates that the antimicrobial peptide was successfully grafted onto the surface of the material.
  • the samples in Examples 1 and 3 were tested for photothermal performance.
  • the samples were immersed in 200 ⁇ L phosphate buffered saline (PBS), and the PBS solution was used as a control.
  • PBS phosphate buffered saline
  • the samples were irradiated with a near-infrared laser with a wavelength of 808 nm and a power of 1.0 W/cm 2.
  • the distance between the near-infrared light source and the sample was 10 cm, and the irradiation time was 10 min.
  • an infrared thermal imaging camera was used to monitor the temperature change.
  • Figure 5a shows the temperature change of the sample surface detected by the infrared thermal imaging camera during the 10 min irradiation of the BP@PLGA sample.
  • Figure 5b shows the temperature change trend of each sample surface within 10 min of near-infrared light irradiation.
  • the horizontal axis is the time of light irradiation, and the vertical axis is the sample surface temperature.
  • the PLGA sample itself has no photothermal conversion ability.
  • the sample surface temperature was below 34°C.
  • the black phosphorus nanosheets added to the BP@PLGA sample can produce a local thermal effect under the irradiation of near-infrared light, increasing the surface temperature of the sample from room temperature to nearly 40°C within 1 minute, and the surface temperature of the sample reached a maximum of 54°C after 10 minutes of irradiation.
  • the surface temperature of the BP@PLGA+A sample can still reach a maximum of about 53°C after 10 minutes of near-infrared light irradiation, indicating that this series of operations of covalently grafting antimicrobial peptides after plasma treatment has little effect on the photothermal performance of the loaded black phosphorus sample.
  • Example 1 The samples (PLGA, BP@PLGA) in Example 1 were subjected to degradation performance tests. The samples were immersed in 1 mL PBS and placed at room temperature. A digital camera was used to record the surface changes of the samples at different immersion days.
  • Figure 6 is a macroscopic image of the surface of the sample after immersion for 14 days. It can be seen from the results in the figure that as the immersion days increase, some holes gradually form on the surface of the film layer, indicating that the film layer is slowly degrading.
  • Figure 7a is a photograph of the survival of Staphylococcus aureus cultured on the surface of each group of samples detected by the plate coating method.
  • the horizontal axis is the sample name
  • the vertical axis is the antibacterial efficiency of the sample against Staphylococcus aureus.
  • Mouse fibroblast L929 cells were inoculated on the surfaces of samples PLGA, BP@PLGA and BP@PLGA+A. After the cell adhesion was stable, some samples of the BP@PLGA+A group were treated with near-infrared light irradiation. The surface temperature of the samples was lower than 42°C under near-infrared light irradiation. The irradiation time was 3 min, and the treatment group was named BP@PLGA+A+L. The treated samples continued to be cultured in the incubator. After culturing for 1 day, 3 days, and 5 days, the proliferation of fibroblasts was detected using the CCK-8 method to detect the biosafety of each sample.
  • the horizontal axis is the cell culture time
  • the vertical axis is the absorbance value at a wavelength of 450 nm.
  • the present invention provides a method for preparing a degradable wound dressing with dual antibacterial efficacy.
  • the flow chart of the method is shown in FIG9 .
  • the antimicrobial peptide grafted on the surface of the dressing not only has excellent antibacterial effect, but also the photothermal agent added to the dressing will produce a thermal effect under the irradiation of near-infrared light, further enhancing the antibacterial effect of the dressing.
  • the dressing Under the superposition of the two antibacterial effects, the dressing has excellent efficacy in resisting wound infection.

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Abstract

L'invention concerne un pansement dégradable ayant des effets antibactériens doubles, son procédé de préparation et son utilisation. Le procédé consiste en : tout d'abord le dopage d'une substance ayant une performance de conversion photo-thermique en un matériau de base de pansement, puis la préparation, au moyen d'un procédé d'évaporation de solvant, d'un film de pansement chargé d'un agent photo-thermique ; la réalisation d'un traitement au plasma sur la surface du film de pansement ; et l'incubation du film de pansement ayant subi le traitement au plasma dans une solution contenant un peptide antibactérien, de façon à greffer le peptide antibactérien à la surface du film de pansement pour obtenir un pansement dégradable ayant des effets antibactériens doubles. Tandis que le pansement présente une bonne sécurité biologique, le peptide antibactérien greffé à la surface du film de pansement a un excellent effet antibactérien, et l'agent photo-thermique ajouté peut générer un effet thermique sous l'irradiation de lumière proche infrarouge, ce qui permet d'améliorer l'effet antibactérien du pansement. Par la combinaison des deux effets antibactériens, le pansement a un excellent effet de résistance à une infection par lésion.
PCT/CN2023/141678 2023-02-01 2023-12-25 Pansement dégradable ayant des effets antibactériens doubles, son procédé de préparation et son utilisation Ceased WO2024159977A1 (fr)

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CN202310049722.5A CN116212084A (zh) 2023-02-01 2023-02-01 具有双重抗菌功效的可降解伤口敷料及其制备方法和应用

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119656366A (zh) * 2024-11-28 2025-03-21 东华大学 一种集成恒温光热效应和可控释药功能的抗菌水凝胶敷料及其制备方法和应用

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116212084A (zh) * 2023-02-01 2023-06-06 中国科学院深圳先进技术研究院 具有双重抗菌功效的可降解伤口敷料及其制备方法和应用
WO2025097421A1 (fr) * 2023-11-10 2025-05-15 松阳生技股份有限公司 Composition pharmaceutique pour favoriser la prolifération des cellules cutanées et la cicatrisation des plaies et son procédé de préparation
CN120661439B (zh) * 2025-08-22 2025-10-28 湖北随州双星生物科技有限公司 一种皮肤愈合用抗菌止血药物组合物及其制备方法

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107189096A (zh) * 2017-04-28 2017-09-22 深圳先进技术研究院 一种高分子材料表面改性方法及其产品和用途
CN109456389A (zh) * 2018-12-25 2019-03-12 苏州大学 一种抗菌肽、抗菌肽水凝胶及其制备方法
CN112316137A (zh) * 2020-09-24 2021-02-05 南京斯泰尔医药科技有限公司 一种抗菌纳米材料Au-AMP-Cy的制备方法和应用
CN112316136A (zh) * 2020-09-23 2021-02-05 南京斯泰尔医药科技有限公司 一种抗菌纳米敷料AuNS-AMP-PEG的制备方法和应用
CN113018507A (zh) * 2021-04-02 2021-06-25 常州大学 一种具有光热性能的抗菌水凝胶及其制备方法和应用
WO2021146771A1 (fr) * 2020-01-20 2021-07-29 Royal Melbourne Institute Of Technology Revêtement antimicrobien
CN113908330A (zh) * 2021-10-14 2022-01-11 中国人民解放军陆军军医大学第二附属医院 一种具有光热抗菌止血特性的复合凝胶的制备方法及其产品和应用
CN116212084A (zh) * 2023-02-01 2023-06-06 中国科学院深圳先进技术研究院 具有双重抗菌功效的可降解伤口敷料及其制备方法和应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108042565B (zh) * 2017-11-02 2020-07-03 深圳先进技术研究院 一种具有抗菌功效的黑磷纳米材料及其制备方法
CN110464882A (zh) * 2019-07-29 2019-11-19 东华大学 一种亲水抗菌可降解的输尿管支架管及其制备方法
GB2588442A (en) * 2019-10-24 2021-04-28 Io Cyte Ltd Antimicrobial material
CN114099759B (zh) * 2020-08-28 2022-07-12 北京化工大学 负载相变材料微粒的纤维创面修复支架及其制备方法和应用
CN114318680B (zh) * 2022-01-18 2023-03-28 郑州大学 一种抗菌可降解薄膜材料及其制备方法和应用

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107189096A (zh) * 2017-04-28 2017-09-22 深圳先进技术研究院 一种高分子材料表面改性方法及其产品和用途
CN109456389A (zh) * 2018-12-25 2019-03-12 苏州大学 一种抗菌肽、抗菌肽水凝胶及其制备方法
WO2021146771A1 (fr) * 2020-01-20 2021-07-29 Royal Melbourne Institute Of Technology Revêtement antimicrobien
CN112316136A (zh) * 2020-09-23 2021-02-05 南京斯泰尔医药科技有限公司 一种抗菌纳米敷料AuNS-AMP-PEG的制备方法和应用
CN112316137A (zh) * 2020-09-24 2021-02-05 南京斯泰尔医药科技有限公司 一种抗菌纳米材料Au-AMP-Cy的制备方法和应用
CN113018507A (zh) * 2021-04-02 2021-06-25 常州大学 一种具有光热性能的抗菌水凝胶及其制备方法和应用
CN113908330A (zh) * 2021-10-14 2022-01-11 中国人民解放军陆军军医大学第二附属医院 一种具有光热抗菌止血特性的复合凝胶的制备方法及其产品和应用
CN116212084A (zh) * 2023-02-01 2023-06-06 中国科学院深圳先进技术研究院 具有双重抗菌功效的可降解伤口敷料及其制备方法和应用

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119656366A (zh) * 2024-11-28 2025-03-21 东华大学 一种集成恒温光热效应和可控释药功能的抗菌水凝胶敷料及其制备方法和应用

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