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WO2024140065A1 - Protéine cry51aa1 et son utilisation dans la lutte contre les nuisibles - Google Patents

Protéine cry51aa1 et son utilisation dans la lutte contre les nuisibles Download PDF

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Publication number
WO2024140065A1
WO2024140065A1 PCT/CN2023/136494 CN2023136494W WO2024140065A1 WO 2024140065 A1 WO2024140065 A1 WO 2024140065A1 CN 2023136494 W CN2023136494 W CN 2023136494W WO 2024140065 A1 WO2024140065 A1 WO 2024140065A1
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WO
WIPO (PCT)
Prior art keywords
protein
cry51aa1
plant
plants
pests
Prior art date
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Ceased
Application number
PCT/CN2023/136494
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English (en)
Chinese (zh)
Inventor
巫永春
郭晨亮
陈波
石德英
李华荣
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qingdao Kingagroot Chemical Compound Co Ltd
Original Assignee
Qingdao Kingagroot Chemical Compound Co Ltd
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Publication of WO2024140065A1 publication Critical patent/WO2024140065A1/fr
Anticipated expiration legal-status Critical
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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protection of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/20—Brassicaceae, e.g. canola, broccoli or rucola
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/60—Malvaceae, e.g. cotton or hibiscus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/04—Insecticides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Definitions

  • Corn and cotton are important food crops and fiber crops in China, respectively. Every year, the food losses and economic losses caused by pests, especially thrips and green stink bugs, are huge, and even worse, they affect the survival of the local population.
  • the main control methods commonly used by people are: agricultural control, chemical control, physical control and biological control.
  • scientists have found through research that by transferring insect-resistant genes encoding insecticidal proteins into plants, some insect-resistant transgenic plants can be obtained to prevent and control plant pests.
  • the Cry51Aa1 protein is present in at least a host cell that produces the Cry51Aa1 protein, and the pest comes into contact with at least the Cry51Aa1 protein by ingesting the host cell.
  • the Cry51Aa1 protein exists in at least the bacteria or transgenic plants that produce the Cry51Aa1 protein, and the pest comes into contact with at least the Cry51Aa1 protein by feeding on the bacteria or the tissues of the transgenic plants, and the growth of the pest is inhibited and/or the pest dies after the contact, thereby controlling the damage to plants.
  • the nucleotide sequence of the Cry51Aa1 protein in Escherichia coli has the nucleotide sequence shown in SEQ ID NO:12-20; the nucleotide sequence of the Cry51Aa1 protein in transgenic Arabidopsis has the nucleotide sequence shown in SEQ ID NO:22-30.
  • the transgenic plant further comprises at least one second nucleotide different from the nucleotide encoding the Cry51Aa1 protein.
  • the second nucleotide encodes other Cry insecticidal proteins, Vip insecticidal proteins, protease inhibitors, lectins, ⁇ -amylases or peroxidases; or the second nucleotide is a dsRNA that inhibits important genes in target insect pests.
  • the present invention also provides a use of the Cry51Aa1 protein in controlling pests.
  • the present invention also provides a method for producing a plant for controlling pests, which comprises introducing into the genome of the plant a gene encoding The nucleotide sequence of the Cry51Aa1 protein.
  • the present invention also provides a method for producing plant seeds for controlling pests, which comprises hybridizing a first plant obtained by the method with a second plant, thereby producing seeds containing a nucleotide sequence encoding the Cry51Aa1 protein.
  • the present invention also provides a method for cultivating a plant for controlling pests, comprising: planting at least one plant seed, wherein the genome of the plant seed comprises a nucleotide sequence encoding the Cry51Aa1 protein;
  • the scutellum is triangular and slightly protruding, yellow-green, with 1 shallow longitudinal stripe in the center.
  • the forewing membrane is translucent dark gray, and the remaining green.
  • the foot is yellow-green, the end of the intestinal segment and the financial segment are darker, and the end of the hind leg segment has brown ring spots.
  • the hind leg segment of the female insect is shorter than that of the male insect, does not exceed the end of the abdomen, and the tarsus segment has 3 sections and the end is black.
  • the egg is 1mm long, yellow-green, long pocket-shaped, and the egg cover is creamy yellow, concave in the center, protruding at both ends, and there is no appendage on the edge.
  • the nymphs are in the fifth instar, similar to the adults.
  • the Cry51Aa1 protein of the present invention is toxic to pests.
  • the plants of the present invention especially corn, soybeans and cotton, contain exogenous DNA in their genomes, wherein the exogenous DNA comprises a nucleotide sequence encoding the Cry51Aa1 protein, and the pests come into contact with the protein by feeding on plant tissues, and the growth of the pests is inhibited and/or death is caused after contact. Inhibition refers to lethality or sublethality.
  • the plants should be morphologically normal and can be cultured under conventional methods for consumption and/or production of the product.
  • the plants can substantially eliminate the need for chemical or biological pesticides (the chemical or biological pesticides are pesticides for the pests targeted by the Cry51Aa1 protein).
  • the target insects in the present invention are mainly green stink bug pests or thrips pests.
  • the Cry51Aa1 protein may have an amino acid sequence as shown in SEQ ID NO: 2-10 in the sequence table.
  • other elements may also be included, such as a protein encoding a selectable marker.
  • exogenous DNA is introduced into plants, such as introducing the gene or expression cassette or recombinant vector encoding the Cry51Aa1 protein into plant cells.
  • Conventional transformation methods include, but are not limited to, Agrobacterium-mediated transformation, microemission bombardment, direct DNA uptake into protoplasts, electroporation or silicon whisker-mediated DNA introduction.
  • FIG. 1 is a construction diagram of the recombinant expression vector pET15b-Cry51Aa1-V1 containing the Cry51Aa1 nucleotide sequence and having insecticidal protein use of the present invention.
  • FIG. 5 is a comparison of feeding of western flower thrips on transgenic Arabidopsis T2 generation (3712) and non-transgenic Arabidopsis (COL).
  • the nucleotide sequences of Cry51Aa1 wild type and its mutants optimized in Escherichia coli and Arabidopsis thaliana were synthesized by Nanjing GenScript Biotechnology Co., Ltd. (SEQ ID NO:11-30).
  • Example 2 Construction of recombinant expression vector and transformation of Escherichia coli with the recombinant expression vector to obtain Cry51Aa1 protein
  • the recombinant expression vector pET15b-Cry51Aa1-V1 constructed in step 1 was transformed into Escherichia coli BL21 (DE3) competent cells (Transgen, China, CAT: CD501) by heat shock method, and the positive clones were picked and cultured in LB liquid medium (tryptone 10g/L, yeast extract 5g/L, NaCl 10g/L, ampicillin 100mg/L) at 37°C and 200r/min for 16h. Then the culture solution was transferred to LB medium at a ratio of 1:20 and cultured at 37°C and 200r/min.
  • LB liquid medium tryptone 10g/L, yeast extract 5g/L, NaCl 10g/L, ampicillin 100mg/L
  • the temperature was lowered to 25°C, 0.5mM IPTG was used to induce expression overnight, and the bacteria were collected by centrifugation at 5000xg.
  • the collected cells were resuspended in Ni-buffer A: 50mM Na 2 CO 3 /NaHCO 3 pH 10, 150mM NaCl, 20mM imidazole, and PMSF with a final concentration of 1mM and 250ul protease inhibitor cocktail were added and mixed. After being disrupted by a high-pressure homogenizer, centrifuged at 43000xg, 4°C for 30min, the supernatant was passed through a Ni column.
  • Example 3 Identification of the anti-insect effect of feeding Cry51Aa1 protein on green stink bugs
  • Cry51Aa1-V1, Cry51Aa1-V2, Cry51Aa1-V4, Cry51Aa1-V6, and Cry51Aa1-V7 proteins all showed resistance activity against green stink bug, and were higher than Cry51Aa1-WT. This activity was sufficient to have an adverse effect on the growth of green stink bug, thereby controlling it in the field.
  • Example 4 Test on resistance of transgenic Arabidopsis thaliana to second-instar nymphs of western flower thrips
  • the transgenic vectors pQY3709 and pQY3711-pQY3719 containing At-Cry51Aa1-WT and V1-V9 were constructed according to conventional methods and successfully transformed into Arabidopsis thaliana.
  • Each Arabidopsis plant was inoculated with 50 healthy and consistent second-instar nymphs of western flower thrips. The observation was continued until the 15th day. The number of surviving adults was investigated and recorded, and the leaf feeding was recorded by taking photos.
  • Non-transgenic Arabidopsis (COL) and transgenic Arabidopsis pQY3709 (At-Cry51Aa1-WT) were used as the control group, and the differences in survival rates between different treatments were compared by univariate analysis.
  • transgenic Arabidopsis were evaluated according to the resistance evaluation criteria of transgenic Arabidopsis to the second-instar nymphs of western flower thrips in Table 2, and the transgenic Arabidopsis plants with a resistance grade of "resistant” were counted as resistant plants. Finally, the proportion of resistant plants to the total number of plants was counted (Table 3).
  • the T2 generation Arabidopsis transformants of typical pQY3712 and pQY3715 were selected for resistance to western flower thrips.
  • Each Arabidopsis plant was inoculated with 50 healthy and consistent second-instar nymphs of western flower thrips. The number of surviving adults was investigated and recorded, and the leaf feeding situation was recorded by taking photos.
  • Non-transgenic Arabidopsis (COL) was used as the control group, and the resistance level was determined according to the resistance evaluation criteria shown in Table 2.
  • Table 4 The resistance ratio of T2 transgenic Arabidopsis to western flower thrips
  • Example 5 Test on resistance of transgenic cotton to second-instar nymphs of western flower thrips
  • the transgenic vectors pQY4340 and pQY5112 containing Cry51Aa1-WT (SEQ ID NO: 1) and Cry51Aa1-V2 (SEQ ID NO: 3) were constructed according to conventional methods and successfully transformed into cotton.
  • Non-transgenic cotton (TM-1) and transgenic cotton pQY4340 (Cry51Aa1-WT) were used as control groups, and the differences in survival rates among different treatments were compared using univariate analysis.
  • the survival of western flower thrips on transgenic cotton is shown in Table 5 (three representative plants were selected for each vector).
  • the T2 generation cotton transformants of the typical representative pQY5112 were selected for the western flower thrips resistance test. 100 healthy and consistent second-instar nymphs of western flower thrips were inoculated into the detached leaves of each cotton plant. The number of surviving adults was recorded and observed until the 7th day. The resistance ratio of cotton plants was calculated.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physiology (AREA)
  • Molecular Biology (AREA)
  • Pest Control & Pesticides (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Insects & Arthropods (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La présente invention concerne l'utilisation d'une protéine insecticide, en particulier d'une protéine Cry51Aa1 et son utilisation dans la lutte contre les nuisibles, en particulier l'Apolygus lucorum ou les thrips. La protéine Cry51Aa1 comprend une séquence d'acides aminés choisie dans le groupe constitué par : SEQ ID NO : 2-10, et génère une excellente résistance aux insectes, et peut en particulier protéger l'ensemble de la plante pendant toute la période de croissance des plantes, de façon à empêcher et à contrôler l'invasion de nuisibles, y compris les thrips et l'Apolygus lucorum. De plus, la protéine Cry51Aa1 est exempte de pollution, exempte de résidus, présente des effets stables et approfondis, et est simple, pratique et économique.
PCT/CN2023/136494 2022-12-29 2023-12-05 Protéine cry51aa1 et son utilisation dans la lutte contre les nuisibles Ceased WO2024140065A1 (fr)

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CN202211708367 2022-12-29
CN202211708367.X 2022-12-29

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011075586A1 (fr) * 2009-12-16 2011-06-23 Dow Agrosciences Llc Combinaisons de protéines insecticides pour contrôler la légionnaire d'automne et la pyrale du maïs, et procédés de gestion de la résistance des insectes
CN104271595A (zh) * 2012-04-06 2015-01-07 孟山都技术公司 对半翅目昆虫种类有毒的蛋白质
CN106146628A (zh) * 2015-03-27 2016-11-23 中国农业科学院棉花研究所 一种人工合成的抗虫蛋白及其相关生物材料与应用
CN107573410A (zh) * 2017-10-25 2018-01-12 中国科学院微生物研究所 一种人工合成的植物抗虫蛋白及其制备方法和应用

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011075586A1 (fr) * 2009-12-16 2011-06-23 Dow Agrosciences Llc Combinaisons de protéines insecticides pour contrôler la légionnaire d'automne et la pyrale du maïs, et procédés de gestion de la résistance des insectes
CN104271595A (zh) * 2012-04-06 2015-01-07 孟山都技术公司 对半翅目昆虫种类有毒的蛋白质
CN106146628A (zh) * 2015-03-27 2016-11-23 中国农业科学院棉花研究所 一种人工合成的抗虫蛋白及其相关生物材料与应用
CN107573410A (zh) * 2017-10-25 2018-01-12 中国科学院微生物研究所 一种人工合成的植物抗虫蛋白及其制备方法和应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
XU CHENGCHEN; CHINTE UNMESH; CHEN LIRONG; YAO QINGQING; MENG YING; ZHOU DAYONG; BI LI-JUN; ROSE JOHN; ADANG MICHAEL J.; WANG BI-CH: "Crystal structure of Cry51Aa1: A potential novel insecticidal aerolysin-type β-pore-forming toxin fromBacillus thuringie", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ELSEVIER, AMSTERDAM NL, vol. 462, no. 3, 7 May 2015 (2015-05-07), Amsterdam NL , pages 184 - 189, XP029141460, ISSN: 0006-291X, DOI: 10.1016/j.bbrc.2015.04.068 *
ZHU LEICHENG: "Insecticidal Effect of Bt Toxin Cry51Aa1 to Apolygus Lucorum and Adelphocoris Suturalis", CHINESE MASTER'S THESES FULL-TEXT DATABASE, 1 June 2016 (2016-06-01), pages 1 - 55, XP093186974 *

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