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WO2024033421A3 - Novel pore monomers and pores - Google Patents

Novel pore monomers and pores Download PDF

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Publication number
WO2024033421A3
WO2024033421A3 PCT/EP2023/072065 EP2023072065W WO2024033421A3 WO 2024033421 A3 WO2024033421 A3 WO 2024033421A3 EP 2023072065 W EP2023072065 W EP 2023072065W WO 2024033421 A3 WO2024033421 A3 WO 2024033421A3
Authority
WO
WIPO (PCT)
Prior art keywords
pores
novel pore
pore monomers
novel
monomers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2023/072065
Other languages
French (fr)
Other versions
WO2024033421A2 (en
Inventor
Elizabeth Jayne Wallace
Pratik Raj SINGH
Ranga Prabhath MALAVIARACHCHIGE RABEL
Richard George HAMBLEY
Lakmal Nishantha JAYASINGHE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Nanopore Technologies PLC
Original Assignee
Oxford Nanopore Technologies PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Nanopore Technologies PLC filed Critical Oxford Nanopore Technologies PLC
Priority to CA3262140A priority Critical patent/CA3262140A1/en
Priority to CN202380056536.2A priority patent/CN119604766A/en
Priority to KR1020257005135A priority patent/KR20250048550A/en
Priority to EP23758524.5A priority patent/EP4569328A2/en
Priority to AU2023322670A priority patent/AU2023322670A1/en
Priority to JP2025507383A priority patent/JP2025526703A/en
Publication of WO2024033421A2 publication Critical patent/WO2024033421A2/en
Publication of WO2024033421A3 publication Critical patent/WO2024033421A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to novel pore monomer conjugates, pore complexes formed from the conjugates and their uses in analyte detection and characterisation.
PCT/EP2023/072065 2022-08-09 2023-08-09 Novel pore monomers and pores Ceased WO2024033421A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CA3262140A CA3262140A1 (en) 2022-08-09 2023-08-09 Novel pore monomers and pores
CN202380056536.2A CN119604766A (en) 2022-08-09 2023-08-09 Novel pore monomers and pores
KR1020257005135A KR20250048550A (en) 2022-08-09 2023-08-09 New pore monomers and pores
EP23758524.5A EP4569328A2 (en) 2022-08-09 2023-08-09 Novel pore monomers and pores
AU2023322670A AU2023322670A1 (en) 2022-08-09 2023-08-09 Novel pore monomers and pores
JP2025507383A JP2025526703A (en) 2022-08-09 2023-08-09 Novel Pore Monomers and Pores

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB2211602.4A GB202211602D0 (en) 2022-08-09 2022-08-09 Novel pore monomers and pores
GB2211602.4 2022-08-09

Publications (2)

Publication Number Publication Date
WO2024033421A2 WO2024033421A2 (en) 2024-02-15
WO2024033421A3 true WO2024033421A3 (en) 2024-03-28

Family

ID=84546330

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2023/072065 Ceased WO2024033421A2 (en) 2022-08-09 2023-08-09 Novel pore monomers and pores

Country Status (8)

Country Link
EP (1) EP4569328A2 (en)
JP (1) JP2025526703A (en)
KR (1) KR20250048550A (en)
CN (1) CN119604766A (en)
AU (1) AU2023322670A1 (en)
CA (1) CA3262140A1 (en)
GB (1) GB202211602D0 (en)
WO (1) WO2024033421A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202405960D0 (en) 2024-04-29 2024-06-12 Oxford Nanopore Tech Plc Photo-active conjugates and their uses
GB202407247D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019002893A1 (en) * 2017-06-30 2019-01-03 Vib Vzw Novel protein pores
US20220056517A1 (en) * 2018-11-08 2022-02-24 Oxford Nanopore Technologies Limited Pore

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US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
AU2008217579A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Formation of lipid bilayers
EP2158476B8 (en) 2007-05-08 2019-10-09 Trustees of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
EP3540436B1 (en) 2007-09-12 2023-11-01 President And Fellows Of Harvard College High-resolution molecular sensor
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
CN102245760A (en) 2008-07-07 2011-11-16 牛津纳米孔技术有限公司 Enzyme-pore constructs
US9222082B2 (en) 2009-01-30 2015-12-29 Oxford Nanopore Technologies Limited Hybridization linkers
JP5781502B2 (en) 2009-04-20 2015-09-24 オックスフォード ナノポール テクノロジーズ リミテッド Lipid bilayer sensor array
CN102741430B (en) 2009-12-01 2016-07-13 牛津楠路珀尔科技有限公司 Biochemical analyzer, for first module carrying out biochemical analysis and associated method
EP2580588B1 (en) 2010-06-08 2014-09-24 President and Fellows of Harvard College Nanopore device with graphene supported artificial lipid membrane
CN104039979B (en) 2011-10-21 2016-08-24 牛津纳米孔技术公司 Hole and Hel308 unwindase is used to characterize the enzyme method of herbicide-tolerant polynucleotide
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
WO2013098562A2 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Enzyme method
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
JP6614972B2 (en) 2012-07-19 2019-12-04 オックスフォード ナノポール テクノロジーズ リミテッド Modified helicase
EP2875152B1 (en) 2012-07-19 2019-10-09 Oxford Nanopore Technologies Limited Enzyme construct
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
JP6375301B2 (en) 2012-10-26 2018-08-15 オックスフォード ナノポール テクノロジーズ リミテッド Droplet interface
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KR20170042794A (en) 2014-09-01 2017-04-19 브이아이비 브이지더블유 Mutant csgg pores
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GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
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CN113773373B (en) 2021-10-12 2024-02-06 成都齐碳科技有限公司 Mutant of porin monomer, protein hole and application thereof
CN113896776B (en) 2021-10-12 2024-02-06 成都齐碳科技有限公司 Mutant of porin monomer, protein hole and application thereof
CN113754743B (en) 2021-10-12 2024-02-02 成都齐碳科技有限公司 Mutant of porin monomer, protein hole and application thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019002893A1 (en) * 2017-06-30 2019-01-03 Vib Vzw Novel protein pores
US20220056517A1 (en) * 2018-11-08 2022-02-24 Oxford Nanopore Technologies Limited Pore

Non-Patent Citations (2)

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VAN DER VERREN SANDER E ET AL: "A dual-constriction biological nanopore resolves homonucleotide sequences with high fidelity", NATURE BIOTECHNOLOGY, vol. 38, no. 12, 1 December 2020 (2020-12-01), pages 1415 - 1420, XP037311062, ISSN: 1087-0156, DOI: 10.1038/S41587-020-0570-8 *
ZHANG MANFENG ET AL: "Cryo-EM structure of the nonameric CsgG-CsgF complex and its implications for controlling curli biogenesis in Enterobacteriaceae", PLOS BIOLOGY, vol. 18, no. 6, 19 June 2020 (2020-06-19), pages e3000748, XP055821231, Retrieved from the Internet <URL:https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pbio.3000748/1/pbio.3000748.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa@plos-prod.iam.gserviceaccount.com/20210705/auto/storage/goog4_request&X-Goog-Date=20210705T155251Z&X-Goog-Expires=86400&X-Goog-SignedHeaders=h> DOI: 10.1371/journal.pbio.3000748 *

Also Published As

Publication number Publication date
EP4569328A2 (en) 2025-06-18
KR20250048550A (en) 2025-04-09
CN119604766A (en) 2025-03-11
JP2025526703A (en) 2025-08-15
GB202211602D0 (en) 2022-09-21
WO2024033421A2 (en) 2024-02-15
CA3262140A1 (en) 2024-02-15
AU2023322670A1 (en) 2025-01-23

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