WO2024015095A1

WO2024015095A1 - Method of treating skin pigmentation and formulation for treatment

Info

Publication number: WO2024015095A1
Application number: PCT/US2022/054352
Authority: WO
Inventors: Jordan PLEWS
Original assignee: Elevai Labs Inc
Current assignee: Elevai Labs Inc
Priority date: 2022-07-14
Filing date: 2022-12-30
Publication date: 2024-01-18
Anticipated expiration: 2025-01-14

Abstract

A method, and formulations for the method, for the treatment of skin pigmentation issues is herein described. Typically, the method involves applying an exosome-based skincare product to skin affected by skin pigmentation issues. The exosome-based skincare product contains exosomes produced by and excreted from human umbilical mesenchymal stem cells cultured in vitro under specific conditions and then contained within a solvent. The skin pigmentation issues may be melasma, hyperpigmentation, rosacea, or vitiligo, and may be located on the face, scalp, chest, or back.

Description

Method of Treating Skin Pigmentation and Formulation for Treatment

BACKGROUND

Field of the Invention

[0001] The present invention relates generally to treatments for skin pigmentation along with products used to treat skin pigmentation. More specifically, the present invention relates to exosome-based methods and products for treating skin pigmentation disorders.

Description of the Related Art

[0002] For many individuals, the appearance of the skin is an important part of individual identity. A person’s skin changing in pigmentation from what is expected, or desired, can create a large amount of stress for the individual. The most common types of skin pigmentation changes are a result of melasma, hyperpigmentation, rosacea, or vitiligo.

[0003] Current treatments for skin pigmentation changes are facial acids, such as alpha hydroxy acids, azelaic acid, salicylic acid, and ascorbic acid, retinoids, chemical peels, laser peels, intense pulse light therapy, dermabrasion and microdermabrasion, and lightening creams. Other treatments for specific skin pigmentation changes include tranexamic acid and platelet-rich plasma injections (for melasma), oral antibiotics or acne drugs (for rosacea), and surgery (for stable vitiligo).

[0004] Many of these treatments have undesirable side effects that may even counteract the desired effects, including furthering the skin discoloration for some individuals. Acids applied incorrectly can bum the skin, resulting in more skin discoloration and severe discomfort in the burned areas; retinoids have been shown to increase sensitivity to UV light. Other treatments require expensive and time-consuming trips to a dermatologist’s office and are rarely singletreatment approaches.

[0005] It would be beneficial to have a single product that is useful in treating all of these reasons for skin discoloration that has limited side effects.

SUMMARY

[0006] In accordance with the embodiments herein, a method for treating skin discoloration, along with formulations for use, is described. The method comprises applying an exosome-based skincare product to skin that has skin pigmentation issues. The exosome-based skincare product contains exosomes produced by and excreted from human umbilical mesenchymal stem cells cultured in vitro under specific conditions and then contained within a solvent. Note that the exosome-based skincare product does not contain live human umbilical mesenchymal stem cells. Optional ingredients such as skin-conditioning agents, antioxidants, surfactants, buffering agents, viscosity decreasing agents, viscosity increasing agents, peptides, binders, and/or humectants may be included.

DETAILED DESCRIPTION OF EMBODIMENTS

[0007] In the following description, for purposes of explanation and not limitation, details and descriptions are set forth in order to provide a thorough understanding of the present invention. However, it will be apparent to those skilled in the art that the present invention may be practiced in other embodiments that depart from these details and descriptions without departing from the spirit and scope of the invention.

[0008] For the purpose of definition, within this document the term “media” is used to mean any substrate which can contain an exosome-based product and is not to be read as the plural form of “medium”. Appropriate substrates for skincare are well known within the industry and as such are not discussed in detail herein.

[0009] For the purpose of definition, within this document, the term “human umbilical mesenchymal stem cells” is used to mean any preparation of human umbilical mesenchymal stem cells, including human umbilical mesenchymal stem cells contained within human umbilical stem cell conditioned media. Within the industry, human umbilical mesenchymal stem cells are known by a number of different names, including but not limited to human Wharton’s Jelly derived mesenchymal stem cells, human Wharton’s Jelly derived mesenchymal stromal cells, human umbilical derived mesenchymal stromal cells, hUMSCs, hWJMSCs, Wharton's Jelly derived multipotent progenitor cells, and umbilical derived multipotent progenitor cells. Additionally, these stem cells can be identified by cell surface markers, such as CD90+, CD105+, CD73+, and CD45-.

[0010] Within this document, the exosomes discussed are much smaller than a typical mesenchymal stem cell, about 1/100th the size of a cell, and have a diameter of approximately 30- 150 nanometers (run). Exosomes are roughly spherical and made up of a lipid bilayer produced by the cell they originated from. This lipid bilayer forms a protective ‘shell’ or outer casing, and within the ‘shell’ or outer casing is the exosome pay load which contains molecules deposited there by the cell that generated the exosome. While exosomes are generated using some of the origin cell’s own cellular material, the exosomes do not contain cells, nor are they explicitly cellular material.

[0011] In an illustrative embodiment of the invention, the method comprises the step of applying an exosome-based skincare product to skin that has skin pigmentation issues. The exosome-based skincare product contains exosomes in or derived from a media conditioned by human umbilical mesenchymal stem cells within a solvent. In optional embodiments, skinconditioning agents, antioxidants, surfactants, buffering agents, viscosity decreasing agents, viscosity increasing agents, peptides, binders, and/or humectants may be included. Other optional ingredients are contemplated and described herein. Frequently, all of these optional ingredients are included with the human umbilical mesenchymal stem cell derived exosomes, or media, and solvent. Additional optional ingredients beyond those explicitly described herein are contemplated.

[0012] In most embodiments, the solvent is water. However, oil-based solvents are contemplated.

[0013] Skin-conditioning agents within the skincare industry are vast and well documented. Any of the well-known skin-conditioning agents work well with the invention and is contemplated. Specifically, glycerin, sodium hyaluronate, niacinamide, panthenol, Caesalpinia spinosa gum, ceramide NP, ceramide AP, ceramide EOP, yeast extract, Citrullus ianatus fruit extract, Pyrus malus fruit extract, Lens esculenta fruit extract, sodium PCA, caprylyl glycol, ethylhexylglycerin, hydrolyzed sodium hyaluronate, and phytosphingosine are frequently used with the invention. [0014] As with skin-conditioning agents, antioxidants are well known and well documented. With the invention, the antioxidants most frequently used are ascorbic acid, magnesium ascorbyl phosphate, ascorbyl glucoside, sodium ascorbyl phosphate, tetrahexyldecyl ascorbate, glutathione, and Camellia sinensis leaf extract. Any other well-known antioxidants in the skincare industry can be and is contemplated.

[0015] Surfactants frequently used with the invention include polysorbate 20, polysorbate 60, and sodium lauroyl lactylate. Any other well-known surfactant in the skincare industry can be used and is contemplated. While not a surfactant itself, cholesterol is commonly used to stabilize the emulsions that are achieved with surfactants. Some embodiments of the exosome-based skincare product include cholesterol.

[0016] Buffering agents, such as sodium lactate, disodium phosphate, and sodium phosphate most frequently used with the invention, are common in the skincare industry. As many skincare products have harsh pH, both acidic and basic, buffers are frequently used to ensure that the final product is of a neutral pH for the skin. Other well-known buffering agents in the skincare industry are acceptable and are contemplated.

[0017] Viscosity increasing and decreasing agents are frequently used in the skincare industry. Those most frequently used with the invention are hydroxyethylcellulose, Caesalpinia spinosa gum, dextran, carbomer, hexylene glycol, and butylene glycol. Any other well-known viscosity increasing or decreasing agent in the skincare industry can be used and is contemplated.

[0018] Peptides are commonly added to skincare products. Peptides such as bis(tripeptide-l) copper acetate, acetyl octapeptide-3, palmitoyl tripeptide-37, palmitoyl pripeptide-1, palmitoyl tetrapeptide-7, acetyl hexapeptide- 8, and trifluoroacetyl tripeptide-2 are most frequently used with the invention. Other peptides well-known in the skincare industry can be used and are contemplated.

[0019] Binders and bulking agents are frequently used in the skincare industry to improve the usability of a product for the consumer. Binders such as hydroxyethylcellulose, dextran, pullulan, and xanthan gum are the binders and bulking agents most frequently used. However, other binders and bulking agents are contemplated, especially those well-known within the skincare industry.

[0020] The benefits of Aloe barbadensis leaf juice for the skin are well studied, well known, and well documented. Some embodiments include Aloe barbadensis leaf juice for its skincare related properties.

[0021] Humectants are frequently used in the skincare industry to prevent the loss of moisture from the skin. Glycerin, sodium hyaluronate, arginine in all of its forms, Nannochloropsis oculate extract, sodium PCA, and hydrolyzed sodium hyaluronate are the most common humectants used. Other humectants well-known within the skincare industry are contemplated.

[0022] Antimicrobials, such as Leuconostoc root ferment filtrate most commonly used, are vitally important to the skincare industry. Other antimicrobials are contemplated, especially those well-known in the skincare industry. [0023] Chelating agents are commonly used within the skincare industry to stabilize the metal ions found in many skincare products. For the exosome-based skincare product, sodium phytate is the most frequently used chelating agent. Other chelating agents, such as ethylenediaminetetraacetic acid, etidronic acid, galactaric acid, sodium metasilicate, and phosphate derivatives, as well as derivatives of each of the previously listed acids, are also used in some embodiments. Other chelating agents known within the skincare industry are contemplated. [0024] Preservatives, such as phenoxyethanol, sodium benzoate, and potassium sorbate, are key to preserving the life of skincare products. Other preservatives, especially those known in the skincare industry, are contemplated.

[0025] In another illustrative embodiment the exosome-based skincare product contains human umbilical mesenchymal stem cell conditioned media between 25.0 and 50.0 percent by mass, water between 0.1 and 60.0 percent by mass, glycerin between 3.0 and 10.0 percent by mass, Aloe barbadensis leaf juice between 1.0 and 5.0 percent by mass, sodium hyaluronate between 1.0 and 5.0 percent by mass, niacinamide between 1.0 and 5.0 percent by mass, panthenol between 1.0 and 5.0 percent by mass, magnesium ascorbyl phosphate between 1.0 and 5.0 percent by mass, ascorbyl glucoside between 0.5 and 3.0 percent by mass, sodium ascorbyl phosphate between 0.1 and 3.0 percent by mass, hydroxyethylcellulose between 0.1 and 5.0 percent by mass, Caesalpinia spinosa gum between 0.1 and 3.0 percent by mass, bis(tripeptide-l) copper acetate between 0.1 and 5.0 percent by mass, polysorbate 20 between 0.1 and 3.0 percent by mass, polysorbate 60 between 0.1 and 3.0 percent by mass, ceramide NP between 0.1 and 3.0 percent by mass, ceramide AP between 0.1 and 3.0 percent by mass, ceramide EOP between 0.1 and 3.0 percent by mass, acetyl octapeptide-3 between 0.1 and 3.0 percent by mass, palmitoyl tripeptide-37 between 0.1 and 3.0 percent by mass, palmitoyl tripeptide- 1 between 0.1 and 3.0 percent by mass, palmitoyl tetrapeptide-7 between 0.1 and 3.0 percent by mass, acetyl hexapeptide-8 between 0.1 and 3.0 percent by mass, trifluoroacetyl tripeptide-2 between 0.1 and 3.0 percent by mass, dextran between 0.1 and 3.0 percent by mass, tetrahexyldecyl ascorbate between 0.1 and 10.0 percent by mass, pullulan between 0.1 and 3.0 percent by mass, arginine between 0.1 and 2.0 percent by mass, glutathione between 0.1 and 2.0 percent by mass, yeast extract between 0.1 and 3.0 percent by mass, Nannochloropsis oculata extract between 0.1 and 3.0 percent by mass, Camellia sinensis leaf extract between 0.1 and 3.0 percent by mass, Citrullus lanatus fruit extract between 0.1 and 3.0 percent by mass, Pyrus malus fruit extract between 0.1 and 3.0 percent by mass, Lens esculenta fruit extract between 0.1 and 3.0 percent by mass, sodium PCA between 0.1 and 3.0 percent by mass, sodium lactate between 0.1 and 3.0 percent by mass, sodium phytate between 0.1 and 3.0 percent by mass, Leuconostoc root ferment filtrate between 0.1 and 3.0 percent by mass, caprylyl glycol between 0.1 and 3.0 percent by mass, ethylhexylglycerin between 0.1 and 3.0 percent by mass, phytosphingosine between 0.1 and 3.0 percent by mass, cholesterol between 0.1 and 3.0 percent by mass, sodium lauroyl lactylate between 0.1 and 3.0 percent by mass, carbomer between 0.1 and 3.0 percent by mass, xanthan gum between 0.1 and 3.0 percent by mass, phenoxyethanol between 0.5 and 3.0 percent by mass, hexylene glycol between 0.1 and 1.0 percent by mass, butylene glycol between 0.1 and 1.0 percent by mass, disodium phosphate between 0.1 and 1.0 percent by mass, sodium phosphate between 0.1 and 1.0 percent by mass, sodium benzoate between 0.1 and 1.0 percent by mass, and potassium sorbate between 0.1 and 1.0 percent by mass. [0026] In a further illustrative embodiment the exosome-based skincare product contains human umbilical mesenchymal stem cell conditioned media between 10.0 and 55.0 percent by mass, water between 1.0 and 30.0 percent by mass, glycerin between 0.1 and 5.0 percent by mass, Aloe barbadensis leaf juice between 0.1 and 5.0 percent by mass, hydroxyethylcellulose between 0.1 and 5.0 percent by mass, hydrolyzed sodium hyaluronate between 0.1 and 5.0 percent by mass, sodium hyaluronate between 1.0 and 5.0 percent by mass, bis(tripeptide-l) copper acetate between 0.1 and 5.0 percent by mass, polysorbate 20 between 0.1 and 3.0 percent by mass, polysorbate 60 between 0.1 and 3.0 percent by mass, ceramide NP between 0.1 and 5.0 percent by mass, ceramide AP between 0.1 and 5.0 percent by mass, ceramide EOP between 0.1 and 5.0 percent by mass, acetyl octapeptide-3 between 0.1 and 5.0 percent by mass, palmitoyl tripeptide-37 between 0.1 and 5.0 percent by mass, palmitoyl tripeptide-1 between 0.1 and 5.0 percent by mass, palmitoyl tetrapeptide-7 between 0.1 and 5.0 percent by mass, acetyl hexapeptide-8 between 0.1 and 5.0 percent by mass, trifluoroacetyl tripeptide-2 between 0.1 and 5.0 percent by mass, dextran between 0.1 and 5.0 percent by mass, pullulan between 0.1 and 3.0 percent by mass, arginine between 0.1 and 3.0 percent by mass, glutathione between 0.1 and 3.0 percent by mass, yeast extract between 0.1 and 3.0 percent by mass, Nannochloropsis oculata extract between 0.1 and 3.0 percent by mass, Camellia sinensis leaf extract between 0.1 and 3.0 percent by mass, Citrullus lanatus fruit extract between 0.1 and 3.0 percent by mass, Pyrus malus fruit extract between 0.1 and 3.0 percent by mass, Lens esculenta fruit extract between 0.1 and 3.0 percent by mass, sodium PCA between 0.1 and 3.0 percent by mass, sodium lactate between 0.1 and 3.0 percent by mass, sodium phytate between 0.1 and 3.0 percent by mass, Leuconostoc root ferment filtrate between 0.1 and 3.0 percent by mass, caprylyl glycol between 0.1 and 3.0 percent by mass, ethylhexylglycerin between 0.1 and 3.0 percent by mass, phytosphingosine between 0.1 and 3.0 percent by mass, cholesterol between 0.1 and 3.0 percent by mass, sodium lauroyl lactylate between 0.1 and 3.0 percent by mass, carbomer between 0.1 and 3.0 percent by mass, xanthan gum between 0.1 and 3.0 percent by mass, phenoxyethanol between 0.5 and 3.0 percent by mass, hexylene glycol between 0.1 and 1.0 percent by mass, butylene glycol between 0.1 and 1.0 percent by mass, disodium phosphate between 0.1 and 1.0 percent by mass, sodium phosphate between 0.1 and 1.0 percent by mass, sodium benzoate between 0.1 and 1.0 percent by mass, and potassium sorbate between 0.1 and 1.0 percent by mass.

[0027] Typically, the skin where the exosome-based skincare product is applied is facial skin, as this is the skin most commonly affected by melasma, problematic hyperpigmentation, and rosacea. However, the skin may also be on the scalp, back, chest, or other areas of skin, such as arms, hands, legs, feet, groin, or buttocks.

[0028] The skin pigmentation issues may be as a result of melasma, hyperpigmentation, rosacea, or vitiligo.

Claims

CLAIMS What is claimed is:

1. A method, comprising: applying an exosome-based skincare product to skin that has skin pigmentation issues.

2. The method of claim 1, wherein the exosome-based skincare product is exosomes created by human umbilical mesenchymal stem cells; and solvent.

3. The method of claim 2, wherein the exosome-based skincare product further comprises at least one skin-conditioning agent.

4. The method of claim 3, wherein the at least one skin-conditioning agent is selected from the group consisting of glycerin, sodium hyaluronate, niacinamide, panthenol, Caesalpinia spinosa gum, ceramide NP, ceramide AP, ceramide EOP, yeast extract, Citrullus ianatus fruit extract, Pyrus malus fruit extract, Lens esculenta Suit extract, sodium PCA, caprylyl glycol, ethylhexylglycerin, hydrolyzed sodium hyaluronate, and phytosphingosine.

5. The method of claim 2, wherein the exosome-based skincare product further comprises at least one antioxidant.

6. The method of claim 5, wherein the at least one antioxidant is selected from the group consisting of ascorbic acid, magnesium ascorbyl phosphate, ascorbyl glucoside, sodium ascorbyl phosphate, tetrahexyldecyl ascorbate, glutathione, and Camellia sinensis leaf extract.

7. The method of claim 2, wherein the exosome-based skincare product further comprises at least one surfactant.

8. The method of claim 7, wherein the at least one surfactant is selected from the group consisting of polysorbate 20, polysorbate 60, and sodium lauroyl lactylate.

9. The method of claim 2, wherein the exosome-based skincare product further comprises at least one buffering agent.

10. The method of claim 9, wherein the at least one buffering agent is selected from the group consisting of sodium lactate, disodium phosphate, and sodium phosphate.

11. The method of claim 2, wherein the exosome-based skincare product further comprises at least one viscosity decreasing agent.

12. method of claim 11, wherein the at least one viscosity decreasing agent is selected from the group consisting of hexylene glycol and butylene glycol.

13. The method of claim 2, wherein the exosome-based skincare product further comprises at least one viscosity increasing agent.

14. The method of claim 13, wherein the at least one viscosity increasing agent is selected from the group consisting of hydroxyethylcellulose, Caesalpinia spinosa gum, dextran, and carbomer.

15. The method of claim 2, wherein the exosome-based skincare product further comprises at least one peptide.

16. The method of claim 15, wherein the at least one peptide is selected from the group consisting of bis(tripeptide-l) copper acetate, acetyl octapeptide-3, palmitoyl tripeptide-37, palmitoyl pripeptide-1, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, and trifluoroacetyl tripeptide-2.

17. The method of claim 2, wherein the exosome-based skincare product further comprises at least one binder.

18. The method of claim 17, wherein the at least one binder is selected from the group consisting of hydroxy ethylcellulose, dextran, pullulan, and xanthan gum.

19. The method of claim 2, wherein the exosome-based skincare product further comprises at least one humectant.

20. The method of claim 19, wherein the at least one humectant is selected from the group consisting of glycerin, sodium hyaluronate, arginine, Nannochlor opsis oculate extract, sodium PCA, and hydrolyzed sodium hyaluronate.

21. The method of claim 1, wherein an individual uses the exosome-based skincare product on the skin of themselves or another individual.

22. The method of claim 1, wherein the exosome-based skincare product comprises exosomes created by human umbilical mesenchymal stem cells wherein the exosomes are between 25.0 and 50.0 percent by mass; water between 0.1 and 60.0 percent by mass; glycerin between 3.0 and 10.0 percent by mass;

Aloe barbadensis leaf juice between 1.0 and 5.0 percent by mass; sodium hyaluronate between 1.0 and 5.0 percent by mass; niacinamide between 1.0 and 5.0 percent by mass; panthenol between 1.0 and 5.0 percent by mass; magnesium ascorbyl phosphate between 1.0 and 5.0 percent by mass; ascorbyl glucoside between 0.5 and 3.0 percent by mass; sodium ascorbyl phosphate between 0.1 and 3.0 percent by mass; hydroxyethylcellulose between 0.1 and 5.0 percent by mass;

Caesalpinia spinosa gum between 0.1 and 3.0 percent by mass; bis(tripeptide-l) copper acetate between 0.1 and 5.0 percent by mass; polysorbate 20 between 0.1 and 3.0 percent by mass; polysorbate 60 between 0.1 and 3.0 percent by mass; ceramide NP between 0.1 and 3.0 percent by mass; ceramide AP between 0.1 and 3.0 percent by mass; ceramide EOP between 0.1 and 3.0 percent by mass; acetyl octapeptide-3 between 0.1 and 3.0 percent by mass; palmitoyl tripeptide-37 between 0.1 and 3.0 percent by mass; palmitoyl tripeptide- 1 between 0.1 and 3.0 percent by mass; palmitoyl tetrapeptide-7 between 0.1 and 3.0 percent by mass; acetyl hexapeptide-8 between 0.1 and 3.0 percent by mass; trifluoroacetyl tripeptide-2 between 0.1 and 3.0 percent by mass; dextran between 0.1 and 3.0 percent by mass; tetrahexyldecyl ascorbate between 0.1 and 10.0 percent by mass; pullulan between 0.1 and 3.0 percent by mass; arginine between 0.1 and 2.0 percent by mass; glutathione between 0.1 and 2.0 percent by mass; yeast extract between 0.1 and 3.0 percent by mass;

Nannochlor opsis oculata extract between 0.1 and 3.0 percent by mass;

Camellia sinensis leaf extract between 0.1 and 3.0 percent by mass;

Citrullus lanatus fruit extract between 0.1 and 3.0 percent by mass;

Pyrus malus fruit extract between 0.1 and 3.0 percent by mass;

Lens esculenta fruit extract between 0.1 and 3.0 percent by mass; sodium PCA between 0.1 and 3.0 percent by mass; sodium lactate between 0.1 and 3.0 percent by mass; sodium phytate between 0.1 and 3.0 percent by mass;

Leuconostoc root ferment filtrate between 0.1 and 3.0 percent by mass; caprylyl glycol between 0.1 and 3.0 percent by mass; ethylhexylglycerin between 0.1 and 3.0 percent by mass; phytosphingosine between 0.1 and 3.0 percent by mass; cholesterol between 0.1 and 3.0 percent by mass; sodium lauroyl lactylate between 0.1 and 3.0 percent by mass; carbomer between 0.1 and 3.0 percent by mass; xanthan gum between 0.1 and 3.0 percent by mass; phenoxyethanol between 0.5 and 3.0 percent by mass; hexylene glycol between 0.1 and 1.0 percent by mass; butylene glycol between 0.1 and 1.0 percent by mass; disodium phosphate between 0.1 and 1.0 percent by mass; sodium phosphate between 0.1 and 1.0 percent by mass; sodium benzoate between 0.1 and 1.0 percent by mass; and potassium sorbate between 0.1 and 1.0 percent by mass.

23. The method of claim 1, wherein the exosome-based skincare product comprises: exosomes created by human umbilical mesenchymal stem cell wherein the exosomes are between 10.0 and 55.0 percent by mass; water between 1.0 and 30.0 percent by mass; glycerin between 0.1 and 5.0 percent by mass;

Aloe barbadensis leaf juice between 0.1 and 5.0 percent by mass; hydroxyethylcellulose between 0.1 and 5.0 percent by mass; hydrolyzed sodium hyaluronate between 0.1 and 5.0 percent by mass; sodium hyaluronate between 1.0 and 5.0 percent by mass; bis(tripeptide-l) copper acetate between 0.1 and 5.0 percent by mass; polysorbate 20 between 0.1 and 3.0 percent by mass; polysorbate 60 between 0.1 and 3.0 percent by mass; ceramide NP between 0.1 and 5.0 percent by mass; ceramide AP between 0.1 and 5.0 percent by mass; ceramide EOP between 0.1 and 5.0 percent by mass; acetyl octapeptide-3 between 0.1 and 5.0 percent by mass; palmitoyl tripeptide-37 between 0.1 and 5.0 percent by mass; palmitoyl tripeptide-1 between 0.1 and 5.0 percent by mass; palmitoyl tetrapeptide-7 between 0.1 and 5.0 percent by mass; acetyl hexapeptide-8 between 0.1 and 5.0 percent by mass; trifluoroacetyl tripeptide-2 between 0.1 and 5.0 percent by mass; dextran between 0.1 and 5.0 percent by mass; pullulan between 0.1 and 3.0 percent by mass; arginine between 0.1 and 3.0 percent by mass; glutathione between 0.1 and 3.0 percent by mass; yeast extract between 0.1 and 3.0 percent by mass;

Nannochlor opsis oculata extract between 0.1 and 3.0 percent by mass;

Camellia sinensis leaf extract between 0.1 and 3.0 percent by mass;

Citrullus lanatus fruit extract between 0.1 and 3.0 percent by mass;

Pyrus malus fruit extract between 0.1 and 3.0 percent by mass;

24. The method of claim 1, wherein the skin is facial skin.

25. The method of claim 1, wherein the skin is selected from the group consisting of scalp, back, and chest skin.

26. The method of claim 1, wherein the skin pigmentation issues are selected from the group consisting of melasma, hyperpigmentation, rosacea, and vitiligo.

27. The method of claim 26, wherein the skin pigmentation issues are located on facial skin.

28. The method of claim 26, wherein the rosacea is located on the skin selected from the group consisting of scalp, back, and chest skin.