WO2024013744A1

WO2024013744A1 - Compositions for tattooing and methods for manufacturing the same

Info

Publication number: WO2024013744A1
Application number: PCT/IL2023/050725
Authority: WO
Inventors: Maor BEN SHLUSH; Izek BEN SHLUSH
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-07-12
Filing date: 2023-07-12
Publication date: 2024-01-18
Anticipated expiration: 2025-01-12

Abstract

Compositions are provided for tattooing that is configured to reduce pain, risk of inflammation and risk of infection during tattooing, and comprise a colored substance and a cannabinoid. The composition for tattooing has either a pain-killing effect, an anti-inflammatory effect, an antibiotic effect, or any combination thereof. The cannabinoid is in a lowest concentration that has an effect on a patient. Additional additives are provided.

Description

COMPOSITIONS FOR TATTOOING AND METHODS FOR MANUFACTURING

THE SAME

FIEED

[001] The present subject matter relates to compositions for tattooing. More particularly, the present subject matter relates to compositions for tattooing comprising at least one cannabinoid.

BACKGROUND

[002] Tattooing is a process of inserting a colored substance, for example ink, dyes, pigments, or mixtures thereof, into the skin to form a design. The colored substance is inserted into the dermis layer of the skin that underlies the epidermis. After initial injection, the colored substance is dispersed throughout a homogenized damaged layer down through the epidermis and upper dermis, where the presence of a foreign material activates phagocytes of the immune system to engulf the colored substance particles. As healing proceeds, the damaged epidermis eliminates colored substance from the surface, while deeper in the skin granulation tissue forms, which is later converted to connective tissue by collagen growth. This process heals the upper dermis, where the colored substance remains trapped within fibroblasts, that ultimately concentrate in a layer just below the dermis-epidermis boundary. As a result, the presence of the colored substance inside the skin tissue is permanent.

[003] Tattooing involves repeatedly piercing the top layer of the skin with a sharp needle covered with the colored substance. Therefore, getting a tattoo is generally a painful process, through people may experience different levels of pain. Inflammation and infection are additional risks of tattooing.

[004] More particularly, piercing of the skin causes cells at the site of piercing to release prostaglandins - substances involved in the induction of inflammation and pain. In addition, nociceptors (pain receptors) at the site of piercing release substance P - a neurotransmitter that transmits a pain signal to the brain. Furthermore, piercing of the skin can allow penetration of microorganisms into the skin, thus causing infection at the site of tattooing, or more severely, penetration of the microorganisms into blood vessels, and delivery of the microorganism to other parts of the body of an individual being tattooed. DESCRIPTION OF THE PREFERRED EMBODIMENTS

[005] Before explaining at least one embodiment in detail, it is to be understood that the subject matter is not limited in its application to the details of construction and the arrangement of the components set forth in the following description or illustrated in the drawings. The subject matter is capable of other embodiments or of being practiced or carried out in various ways. Also, it is to be understood that the phraseology and terminology employed herein is for the purpose of description and should not be regarded as limiting. In discussion of the various figures described herein below, like numbers refer to like parts. The drawings are generally not to scale.

COMPOSITION FOR TATTOOING

[006] One aim of the present subject matter is to provide a composition for tattooing that is configured to reduce pain, risk of inflammation and risk of infection during tattooing. According to one embodiment, the composition for tattooing has either a pain-killing effect, an anti-inflammatory effect, an antibiotic effect, or any combination thereof.

[007] Another aim of the present subject matter is to provide a composition for tattooing that comprises a mixture of a colored substance and a cannabinoid. During the tattooing process, the cannabinoid is injected into the dermis layer of a patient together with the colored substance. As a result, the cannabinoid is absorbed by blood vessels in the area of injection, either immediately, or by slow release, for example during two hours after injection. The cannabinoid has either a local effect of reducing the level of pain experienced by the patient, or a systemic effect on the patient, having a relaxing and calming effect on the patient, or both local and systems effects, during the tattooing process, after the tattooing process, and during as well as after the tattooing process.

[008] According to the present subject matter, the composition for tattooing has to fulfill several requirements. One requirement is that the composition for tattooing would be homogenous. Another requirement is that the colored substance that is in the composition for tattooing would give rise to at least a standard quality, preferably a higher quality, of tattoos compared to other tattooing mixtures used in the art of tattooing. Yet another requirement is that the cannabinoid that is in the composition for tattooing would give rise to the local effect, or the systemic effect, or both the local and systemic effects, as detailed above, when injected into the dermis layer of the skin of the patient. Still another requirement is that the composition for tattooing would be suitable for injection into the dermis layer of the skin of the patient, in terms of health concerns. In other words, the composition for tattooing should be safe for injection into the dermis layer of the skin of the patient, and would not pose any health risks to the patient.

[009] Another requirement is that the composition for tattooing has to have a shelf life that allows usage of the composition for tattooing at least immediately after the preparation of the composition. Another optional requirement is that the shelf life of the composition for tattooing is at least similar to the shelf life, in terms of time period of storage and storage conditions, compared to other tattooing mixtures used in the art of tattooing. An additional optional requirement is that the shelf life to the composition for tattooing is higher compared to other tattooing mixtures used in the art of tattooing, in terms of time period of storage and storage conditions. Thus, the aforementioned features of the composition for tattooing, namely, homogeneity, tattoo quality, effects of the cannabinoid, and safety concerns, have to remain stable during the shelf life of the composition for tattooing.

[0010] According to one embodiment, the shelf life of the composition for tattooing is at least substantially an hour after preparation of the composition. According to another embodiment, the shelf life of the composition for tattooing is up to substantially six months after preparation. According to yet another embodiment, the shelf life of the composition for tattooing is up to substantially a year after preparation. According to still another embodiment, the shelf life of the composition for tattooing is up to substantially two years after preparation. According to a further embodiment, the shelf life of the composition for tattooing is up to substantially three years after preparation. According to an additional embodiment, the composition for tattooing is stable under sunlight during its shelf life. According to yet an additional embodiment, the composition for tattooing is stable at ambient temperature during its shelf life.

[0011] Another aim of the present subject matter is to provide a method for manufacturing a composition for tattooing that comprises a mixture of a colored substance and a cannabinoid, that fulfills the aforementioned requirements. [0012] The term “patient” used herein refers to an individual that is being tattooed, for example an animal, and more particularly a human being.

[0013] The term “colored substance” used herein refers to any type of colored substance that is used for tattooing. Synonymous terms for "colored substance" are pigment, dye, colorant, and the like. Generally, a colored substance is made of at least one metal salt, or plastic material (synthetic material), or organic material (a material from an organic origin - animals, plants, algae, and the like), and any combination thereof.

[0014] Colored substances for tattooing are provided in several colors. Here are some example of tattoo colors and materials of which the corresponding colored substances are made:

[0015] A black colored substance is made, for example, from either iron oxide (FC3O4 or FeO), carbon, logwood, or any combination thereof.

[0016] A brown colored substance is made, for example, from either ochre, iron (ferric) oxides mixed with clay, or a combination thereof.

[0017] A red colored substance is made, for example, from either cinnabar, cadmium red, iron oxide (Fe2O3), napthol-AS pigment, or any combination thereof.

[0018] An orange colored substance is made, for example, from either disazodiarylide, disazopyrazolone, cadmium selenosulfide, or any combination thereof.

[0019] A yellow colored substance is made, for example, from either cadmium yellow, ochres, curcuma yellow, chrome yellow, disazodiarylide, or any combination thereof.

[0020] A green colored substance is made, for example, from either chromium oxide (CnCh), anadomis green, malachite, ferrocyanides, lead chromate, monoazo pigment, Cu/Al phthalocyanine, Cu phthalocyanine, or any combination thereof.

[0021] A blue colored substance is made, for example, from either azure blue, cobalt blue, Cu- phthalocyanine, or any combination thereof.

[0022] A violet colored substance is made, for example, from either manganese ammonium pyrophosphate, various aluminum salts, quinacridone, dioxazine, carbazole, or any combination thereof. [0023] A white colored substance is made, for example, from either lead carbonate, titanium dioxide, barium sulfate, or zinc oxide, or any combination thereof.

[0024] The term “cannabinoid” used herein refers to any compound that is found in a cannabis plant, or hemp, in any part of the cannabis or hemp plant, and a derivative of the compound, for example synthetic derivative. Some examples of cannabinoids include, but not limited to: tetrahydrocannabinol (THC), tetrahydrocannabinolic acid (THCA), cannabidiol (CBD), cannabidiolic acid (CBDA), cannabigerol (CBG), cannabigerolic acid (CBGA), cannabinol (CBN), cannabichromene (CBC), cannabicyclol (CBL), cannabivarin (CBV), tetrahydrocannbivarin (THCV), cannabidivarin (CBDV), cannabichromevarin (CBCV), cannabigerovarin (CBGV), cannabigerol monomethyl ether (CBGM), cannabielsoin (CBE), cannabicitran (CBT). and the like. Thus, the term “cannabinoid” refers to a single compound that is found in cannabis, or to a mixture of a plurality of compounds that are found in cannabis, or hemp.

[0025] According to some embodiments, the term “cannabinoid” refers to an extract of an entire cannabis, or hemp plant. According to some other embodiments, the term “cannabinoid” refers to an extract of a part of cannabis, or hemp plant, for example, flowers extract, leaves extracts, stems extracts, or any combination thereof. An extract of an entire cannabis, or hemp, plant, or a part of the plant, comprises the entire array of cannabinoids that are present in the plant, or part of the plant, as well as other substances from the plant that can increase the effect of the cannabinoids, for example terpenes.

[0026] Any type of extract of the cannabis, or hemp, plant, or the plant parts, is under the scope of the present subject matter. Some exemplary extracts include: oil extract, winterization (oil refinement) extract alcohol extract, butane extract, butane honey oil extract, isopropyl extract, aqueous extract (with water), CO2 extract, supercritical CO2 extract, heat and pressure extract, an extract which is a product of a distillation process, and the like.

[0027] The term "cannabinoid" further refers to an extract from of an entire cannabis, or hemp plant, or at least one plant part, when the plant, or plant part, is at any condition that is suitable for extraction of a cannabinoid, for example, live plant, dried plant, frozen plant, and the like. [0028] Furthermore, any state of the cannabinoid that is used for the preparation of the composition for tattooing is under the scope of the present subject matter, for example, liquid state; solid state, like powder, and the like.

[0029] Additionally, any manufacturing source of the cannabinoid is under the scope of the present subject matter, for example commercially available cannabinoid, self-manufactured cannabinoid, and the like.

[0030] Cannabinoids are known for their psychological and physiological effects on humans that consume them. For example, cannabinoids are known as having anti-inflammatory effects, pain relief effects, antibiotic effects, relaxing and calming effects, and other additional therapeutic effects.

[0031] To summarize, any known type of cannabinoid that fulfills the requirements described here, is under the scope of the present subject matter.

[0032] The term “delivery agent” as used herein refers to any liquid that is configured to mix with a colored substance and a cannabinoid to form a homogenous liquid, in a manner that allows delivery of a mixture of the colored substance and the cannabinoid into the dermis of the patient during tattooing. The delivery agent mixes with the colored substance and the cannabinoid to form a homogenous liquid in any mechanism, for example, dissolving, suspending, encapsulating, encircling, surrounding, linking to, or connecting to the colored substance and the cannabinoid, and any combination thereof. According to one embodiment, the delivery agent comprises one type of liquid, for example an alcohol, oil and the like. According to another embodiment, the delivery agent is a mixture of a plurality of liquids, in other words more than one type of liquid, for example water and alcohol, water and oil and the like. More details about the delivery agent of the present subject matter are disclosed hereinafter.

[0033] According to one embodiment, the delivery agent is configured to dissolve the colored substance and the cannabinoid. Since cannabinoids are soluble in fat, the delivery agent has to be configured to dissolve fatty compounds, as well as the colored substance. Exemplary delivery agents that are configured to dissolve cannabinoids are ethyl alcohol, glycerol, propylene glycol, and the like. Accordingly, the colored substance is also configured to dissolve in these delivery agents that are configured to dissolve cannabinoids. Additional delivery agents can be used as they are commonly used in the art of tattooing, even though they are toxic to some extent, for example, denatured alcohols, methyl alcohol, isopropyl alcohol, ethylene glycol, aldehydes like formaldehyde and gluteraldehyde, as well as various surfactants and detergents.

[0034] According to one embodiment, the colored substance is provided as a solution of the colored substance in a delivery agent. Therefore, the composition for tattooing comprises, in this embodiment, a colored substance and a cannabinoid, wherein the colored substance is dissolved in a delivery agent, for example, a priori dissolved in a delivery agent.

[0035] According to another embodiment, the delivery agent is an aqueous delivery agent, for example, water. There are colored substances that are soluble in water. However, cannabinoids are not soluble in water because of the oily nature of the cannabinoids. Therefore, in some embodiments, the water is mixed with at least one another delivery agent that is configured to dissolve a colored substance and a cannabinoid, for example at least one of the aforementioned delivery agents that are configured to dissolve a colored substance and a cannabinoid, like ethyl alcohol, glycerol, propylene glycol, denatured alcohols, methyl alcohol, isopropyl alcohol, ethylene glycol, aldehydes like formaldehyde and gluteraldehyde, as well as various surfactants and detergents. Furthermore, some additional examples of the delivery agent include: Quillaja extract, citric acid, and sodium benzoate. Any ratio of the aforementioned types of the delivery agent in a mixture thereof is under the scope of the present subject matter.

[0036] However, there are cases when there is a need to use a delivery agent that is only water, or a mixture of water and at least one another delivery agent. For example, when the colored substance is provided as a solution of the colored substance in water, or in a mixture of water and at least one another delivery agent, for example a mixture of water and ethanol, when, for example, the concentration of the ethanol is low. In such cases, the cannabinoid cannot dissolve in the delivery agent. In such cases, another type of a delivery agent is included in the composition for tattooing - an emulsifier, for stabilizing the composition for tattooing in a form of an emulsion. In other words, according to some embodiments, the delivery agent is an emulsifier.

[0037] The term “emulsion” as used herein refers to a mixture of two or more liquids, which do not naturally mix together. For example, an emulsion according to the present subject matter, is a mixture of water and an oily substance, for example a cannabinoid as mentioned above, an oil, or a colored substance that has an oily nature.

[0038] The term “emulsifier” as used herein refers to a substance that stabilizes an emulsion. More specifically, the emulsifier enables a substance having an oily nature to be uniformly dispersed in an aqueous liquid, for example water, as an emulsion. Some exemplary emulsifiers include lecithin - extracted, for example, from soybean, sunflower, olives, eggs, and the like; algin, carrageenan and agar that are extracted from algae; egg yolk, bile extract, soap, albumin, alginates, sodium stearoyl lactylate, glycerol, ammonium phosphatide, monosodium phosphate, locust bean gum, xanthan gum, mustard, monoglycerides, diglycerides, casein, cholic acid, deoxycholic acid, polysorbates, carrageenan, propylene glycol, cetyl alcohol, Irish moss (Chondrus crispus), diacetyl tartaric acid esters, monostearate, guar gum, canola, alcohol, acacia, tragacanth, pectin, starch, taurocholic acid, and the like. Thus, the delivery agent as well.

[0039] According to some embodiments, the delivery agent is an oil. Thus, according to one embodiment, the cannabinoid is provided a priori dissolved in an oil, whereas according to another embodiment, the oil is added to the composition for tattooing during the preparation of the composition for tattooing. Any type of oil is under the scope of the present subject matter, for example, but not limited to: olive oil, flax oil (also known as linen oil), palm oil, soybean oil, canola oil, medium-chain triglyceride (MCT) oil, avocado oil, grape seed oil, hemp oil, coconut oil, fractionated coconut oil, sesame oil, and the like, and any combination thereof.

[0040] According to the embodiment described above, the composition for tattooing comprises at least one cannabinoid. According to another embodiment, the composition for tattooing comprises at least one terpene. Each of cannabinoid and terpene, separately, can reduce pain, risk of inflammation and risk of infection during tattooing. In other words, each of cannabinoid and terpene, separately, has either a pain-killing effect, an anti-inflammatory effect, an antibiotic effect, or any combination thereof. According to a further embodiment, the composition for tattooing comprises a combination of at least one cannabinoid and at least one terpene.

[0041] The term “terpene” as used herein refers to a highly volatile unsaturated hydrocarbon having the formula (CsHsjn, that is produced predominantly by plants. More than 30,000 terpenes are known. Terpenes are also added to cannabinoids, as it has been found that cannabinoid-terpene interactions can produce synergy with respect to various effects on a consumer, for example the treatment of pain, inflammation, depression, anxiety, fungal and bacterial infections, and the like. According to one embodiment, the terpene is from any plant. According to another embodiment, the terpene is from cannabis. According to yet another embodiment, the terpene is from hemp.

[0042] Some exemplary terpenes that can be added to the composition for tattooing include: myrcene, linalool, terpinolene and caryophyllene. These terpenes have a calming effect, and in addition they have a disinfecting effect and a therapeutic effect on the skin, thus rendering them suitable for the purposes of the composition for tattooing of the present subject matter. Additional effects of the terpenes include an awaking, or arousing effect; an anesthetic effect, which is well suitable for tattooing, and the like. In addition, integration of terpenes with a cannabinoid can give rise to increased beneficial effects on the patient during, or after, or during and after, tattooing.

[0043] According to one embodiment, the composition for tattooing further comprises at least one flavonoid.

[0044] The term “flavonoid” as used herein refers to a polyphenolic secondary metabolite found in plants. Chemically, flavonoids have a general structure of a 15-carbon skeleton, which has two phenyl rings (A and B) and a heterocyclic ring (C, the ring containing and embedded oxygen). This carbon structure is also known as C6-C3-C6. The term "flavonoid" a used herein include any type of known flavonoid from any plant, one flavonoid, and a mixture of multiple flavonoids. More particularly, the composition for tattooing comprises at least one flavonoid from a cannabis plant. Some exemplary flavonoids from cannabis include: cannflavine A, cannflavine B, cannflavine C, vitexin, isovitexin, apigenin, kaempferol, quercetin, luteolin, and orientin.

[0045] According to some embodiments, the composition for tattooing has any form that is suitable for delivery into the dermis layer of the skin during tattooing. Some exemplary forms of the composition for tattooing include, but not limited to: liquid solution, liquid suspension, lotion, gel, cream, ointment, paste, and the like.

[0046] According to one embodiment, the composition for tattooing comprises a colored substance and a cannabinoid. According to another embodiment, the composition for tattooing comprises the lowest concentration of cannabinoid that has an effect on the patient, as described above, while the rest of the composition for tattooing is the colored substance. In other words, the composition of tattooing comprises a microdosage of cannabinoid, while the rest of the composition for tattooing is the colored substance. Any concentration of cannabinoid that has an effect on the patient, as described above, is under the scope of the present subject matter. Some exemplary microdosages of cannabinoid in the composition for tattooing can be less than substantially 10 mg, or less than substantially 5 mg, or less than substantially 3 mg, or less than substantially 1 mg, or less than substantially 0.5 mg, or substantially 0.1 mg, or substantially 0.01 mg or less, or substantially 0.001 mg or less, or substantially 0.0001 mg or less, total amount of cannabinoid injected into the patient's skin. It should be noted that different patients are affected differently one from the other to a cannabinoid. On the other hand, the effect of cannabinoid that is desired with the composition for tattooing is in some embodiments local, namely a local effect on the site of injection in the skin, for example to relief pain. Therefore, the amount of cannabinoid in the composition for tattooing is an amount that gives rise to a local effect on the patient.

[0047] According to one embodiment, the concentration of cannabinoid in the composition for tattooing is at least substantially, 0.001% 0.01%, or at least substantially 0.05%, or at least substantially 0.1%, or at least substantially 0.5%, or at least substantially 1%.

[0048] According to one embodiment, the concentration of the cannabinoid in the composition for tattooing is maximal, as long as the amount of the colored substance in the composition for tattooing is high enough to give rise to a desired coloring of the tattoo. Thus, according to this embodiment, the concentration of cannabinoid in the composition for tattooing is up to substantially 99.99%, or to up to substantially 99.95%, or up to substantially 99.9%, or up to substantially 99.5%, or up to substantially 99%.

[0049] According to one embodiment, any concentration of the colored substance in the range of substantially 0.01-99.99 % colored substance, and any concentration of the cannabinoid in the range of substantially 99.99-0.01%, in the composition for tattooing, is under the scope of the present subject matter.

[0050] According to one embodiment, the composition for tattooing further comprises a delivery agent. In other words, according to this embodiment, the composition for tattooing comprises: a colored substance, a cannabinoid, and a delivery agent. Any type of delivery agent as detailed above, and any mixture thereof, is under the scope of the present subject matter. According to another embodiment, the concentration of the delivery agent in the composition for tattooing is suitable for allowing the delivery agent to have its desired at least one effect, as described above.

[0051] According to some embodiments, the composition for tattooing further comprises a terpene. Thus, according to one embodiment, the composition for tattooing comprises a colored substance, a cannabinoid, and a terpene. According to another embodiment, the composition for tattooing comprises a colored substance, a cannabinoid, a delivery agent, and a terpene. Any type of terpene as detailed above, and any mixture thereof, is under the scope of the present subject matter. According to yet another embodiment, the concentration of the terpene in the composition for tattooing is suitable for allowing the terpene to have its desired at least one effect. As described above.

[0052] According to one embodiment, any aforementioned embodiment of the composition for tattooing further comprises water. The addition of water to the composition for tattooing, can be, for example, in order to dilute the colored substance, or to make the composition for tattooing less viscous, for example in case when the composition for tattooing is too viscous, and the like.

[0053] Following are some embodiments of the composition for tattooing.

[0054] According to one embodiment, the composition for tattooing comprises: substantially 98% colored substance; substantially 1% cannabinoid; and substantially 1% emulsifier.

[0055] According to one embodiment, the composition for tattooing comprises: substantially 50% colored substance; substantially 20% emulsifier; and substantially 30% cannabinoid.

[0056] According to one embodiment, the composition for tattooing comprises: substantially 50% colored substance; substantially 48% oil; substantially 1% emulsifier; substantially 1% terpene; and substantially 1% cannabinoid.

[0057] According to one embodiment, the composition for tattooing comprises: substantially 1% colored substance; substantially 98% terpene; and substantially 1% cannabinoid.

[0058] It has been found during experimentation that an addition of maltodextrin, for example Tapioca maltodextrin, and more particularly, Tapioca maltodextrin N-Zorbit M, to the composition for tattooing gives satisfying results in terms of homogeneity and fluidity of the composition for tattooing. Maltodextrin, in general, and Tapioca maltodextrin N-Zorbit M, in particular, among its various effects, stabilizes fatty substances by transforming the fatty substances into powders. The maltodextrin, and Tapioca maltodextrin N-Zorbit M to a better extent, was found to improve the quality of the composition for tattooing, as detailed hereinafter. Therefore, according to one embodiment, the composition for tattooing further comprises maltodextrin. According to another embodiment, the composition for tattooing further comprises Tapioca maltodextrin N-Zorbit M.

[0059] Another aspect of the present subject matter is a method for preparing a composition for tattooing according to embodiments of the present subject matter.

[0060] According to one embodiment, the method for preparing a composition for tattooing comprises: mixing a cannabinoid and colored substance.

[0061] According to another embodiment, the method for preparing a composition for tattooing further comprises: mixing with a delivery agent.

[0062] According to yet another embodiment, the method for preparing a composition for tattooing further comprises: mixing with a terpene. [0063] According to still another embodiment, the method for preparing a composition for tattooing further comprises: mixing with maltodextrin, for example Tapioca maltodextrin N- Zorbit M.

[0064] According to a further embodiment, the method for preparing a composition for tattooing further comprises: mixing with a flavonoid.

[0065] According to one embodiment, when the cannabinoid is in an acidic form it requires decarboxylation, in order to activate the cannabinoid. The decarboxylation of the cannabinoid can be achieved for example by heating the cannabinoid at high temperature. Any heating method is under the scope of the present subject matter, for example cooking, baking and the like. Some exemplary acidic forms of cannabinoids and their respective decarboxylated forms include THCA that is carboxylated to THC, THC can be further decarboxylated to CBN, CBDA that is decarboxylated to CDB, and CBGA that is decarboxylated to CBG.

[0066] Some exemplary decarboxylation processes of THCA to THC include: heating at substantially 121°C, for substantially 25-30 minutes; heating at substantially 115°C for substantially 40 minutes; heating at substantially 107°C, for substantially 45 minutes; heating at substantially 104°C, for substantially 90 minutes; heating at substantially 80-85°C for substantially 20-30 minutes, and the like. THC can be decarboxylated to CBN by heating at substantially 115°C for substantially 180 minutes. CBDA can be dcarboxylated to CBD by heating at substantially 115°C for substantially 90 minutes. CBGA can be decarboxylated to CBG by heating at substantially 104°C for substantially 60 minutes. It should be noted, though, that some decarboxylated forms of cannabinoids are commercially available, for example, CBN. CBD, and CBG. To summarize, any decarboxylation method that gives rise to an active cannabinoid is under the scope of the present subject matter. Nevertheless, it should be further noted that the decarboxylation of THCA to THC can occur at ambient temperature, for a long period of time, for example several months, a year, and the like.

[0067] Thus, according to one embodiment, the method for preparing a composition for tattooing further comprises decarboxylating the cannabinoid, when the cannabinoid is in an acidic form. According to another embodiment, the decarboxylating the cannabinoid comprises heating the cannabinoid. According to yet another embodiment, the heating of the cannabinoid

1 J is at substantially 104°C. According to still another embodiment, the heating of the cannabinoid is for substantially 90 minutes.

[0068] According to one embodiment, the delivery agent is an oil. According to another embodiment, the delivery agent is an emulsifier. According to yet another embodiment, the delivery agent is a mixture of an oil and an emulsifier.

[0069] Thus, when the delivery agent is a mixture of an oil and an emulsifier, there is a need to infuse the cannabinoid with the oil and the emulsifier. This can be done by heating the mixture of the cannabinoid, oil and emulsifier, for example at substantially 90°C. Any time of heating that allow infusion of the cannabinoid with the oil and emulsifier is under the scope of the present subject matter. Some exemplary heating times include: substantially 30 minutes, at least substantially 30 minutes, substantially 90 minutes, substantially 2 hours, at least substantially 2 hours, substantially 4-6 hours, substantially 6-8 hours, and the like.

[0070] Thus, according to one embodiment, when the method for preparing a composition for tattooing comprises mixing the cannabinoid with an oil and an emulsifier, the method further comprises infusing the cannabinoid with the oil and the emulsifier. According to another embodiment, the infusing comprises heating of the mixture of the cannabinoid, the oil and the emulsifier. According to yet another embodiment, the heating is at substantially 30 minutes, or at least substantially 30 minutes, or substantially 90 minutes, or substantially 2 hours, or at least substantially 2 hours, or substantially 4-6 hours, or substantially 6-8 hours,

[0071] Therefore, according to one embodiment, the method for preparing a composition for tattooing comprises: mixing a cannabinoid with an oil and an emulsifier to obtain a mixture of a cannabinoid, an oil and an emulsifier; infusing the mixture of the cannabinoid, the oil and the emulsifier, as described above; and mixing the mixture with a colored substance.

[0072] According to another embodiment, the method further comprises decarboxylating the cannabinoid, for example when the cannabinoid is THCA, as described above. COMPOSITION FOR TATTOOING - EXAMPLES

Exemplary composition for tattooing No. 1

[0073] An exemplary composition for tattooing No. 1 comprises:

0.1 gr THCA - 1.6%

0.24 gr olive oil - 3.9%

0.02 gr soybean lecithin - 0.3%

0.02 gr myrcene - 0.3%

0.02 gr linalool - 0.3%

0.02 gr terpinolene - 0.3%

0.02 gr caryophyllene 0.3%

5.736 gr black tattoo ink - 92.9%

Total weight: 6.176 gr

[0074] Composition for tattooing No. 1 was used for tattooing and gave rise to satisfactory results in terms of homogeneity and fluidity of the composition for tattooing, the tattoo that was obtained, calming during tattooing, and healing after the tattooing. More particularly, After tattooing with the exemplary composition for tattooing No. 1, local pain at the site of tattooing was lower than with a prior art composition, thus implicating that the composition for tattooing had a pain relief effect; and the patient felt relaxed and calm during and after tattooing. These effects lasted for several hours after tattooing, thus indicating that there was slow release of the cannabinoid from the site of injection to the bloodstream of the patient.

Exemplary composition for tattooing No. 2

[0075] An exemplary composition for tattooing No. 2 comprises: cannabinoid ethanol colored substance

Exemplary composition for tattooing No. 3

[0076] An exemplary composition for tattooing No. 3 comprises: cannabinoid ethanol terpene colored substance

Exemplary composition for tattooing No. 4

[0077] An exemplary composition for tattooing No. 2 comprises: cannabinoid ethanol terpene colored substance

Exemplary composition for tattooing No. 5

[0078] An exemplary composition for tattooing No. 5 comprises:

0.101 gr cannabinoid - 0.9%

0.283 gr olive oil - 2.4%

0.04 gr soybean lecithin - 0.3%

0.1045 gr Tapioca maltodextrin N-Zorbit M - 0.9%

11.174 gr black tattoo ink - 95.5%

Total weight: 11.7025 gr

[0079] In exemplary composition for tattooing No. 5, in one version the cannabinoid was CBD, in another version the cannabinoid was THC, and in yet another version the cannabinoid was a mixture of CBD, CBG, and CBN.

[0080] Composition for tattooing No. 5, in its three versions, was used for tattooing and gave rise to satisfactory results in terms of homogeneity and fluidity of the composition for tattooing, the tattoo that was obtained, calming during tattooing, and healing after the tattooing.

Exemplary composition for tattooing No. 6

[0081] An exemplary composition for tattooing No. 6 comprises:

0.101 gr THCA - 0.90%

0.283 gr olive oil - 2.40%

0.1045 gr Tapioca maltodextrin N-Zorbit M - 0.89%

0.04 gr soybean lecithin - 0.34%

0.02 myrcene - 0.17%

0.02 gr linalool - 0.17% 0.02 gr terpinolene - 0.17%

0.02 gr caryophyllene - 0.17%

11.174 gr tattoo ink - 94.84%

Total weight: 11.7825 gr

Exemplary composition for tattooing No. 7

[0082] An exemplary composition for tattooing No. 7 comprises:

0.1 gr CBD - 0.84%

0.05 gr CBG - 0.42%

0.1 gr CBN - 0.84%

0.283 gr olive oil - 2.37%

0.1045 gr Tapioca maltodextrin N-Zorbit M - 0.88%

0.04 gr soybean lecithin - 0.34%

0.02 myrcene - 0.17%

0.02 gr linalool - 0.17%

0.02 gr terpinolene - 0.17%

0.02 gr caryophyllene - 0.17%

11.174 tattoo ink - 93.65%

Total weight: 11.9315 gr

Exemplary composition for tattooing No. 8

[0083] An exemplary composition for tattooing No. 8 comprises:

0.1 gr CBD - 0.84%

005 gr CBG - 0.42%

0.1 gr CBN - 0.84%

0.283 gr olive oil - 2.39%

0.1045 gr Tapioca maltodextrin N-Zorbit M - 0.88%

0.04 gr soybean lecithin - 0.34%

11.174 gr tattoo ink - 94.28%

Total weight: 11.8515 gr

Exemplary composition for tattooing No. 9

[0084] An exemplary composition for tattooing No. 9 comprises:

0.101 gr CBD - 0.90% 0.283 gr olive oil - 2.40%

0.1045 gr Tapioca maltodextrin N-Zorbit M - 0.90%

0.04 gr soybean lecithin - 0.30%

11.174 gr tattoo ink - 95.50%

Total weight: 11.7025 gr

Exemplary composition for tattooing No. 10

[0085] An exemplary composition for tattooing No. 10 comprises:

0.101 gr THCA - 0.90%

0.283 gr olive oil - 2.40%

0.1045 gr Tapioca maltodextrin N-Zorbit M - 0.90%

0.04 gr soybean lecithin - 0.30%

11.174 gr tattoo ink - 97.60%

Total weight: 11.558 gr

Exemplary composition for tattooing No. 11

[0086] An exemplary composition for tattooing No. 11 comprises:

0.1020 gr CBD

0.0512 gr CBG

0.1013 gr CBN

0.5433 gr olive oil

0.0515 gr soybean lecithin

The mixture was infused at substantially 90°C for substantially 110 minutes.

The mixture was briefly vortexed.

0.2022 gr Tapioca maltodextrin N-Zorbit M was added to the mixture, and the mixture was mixed until it became solid.

[0087] Solid pieces of the composition were dissolved in water by repeated mixing to obtain a solution of the composition. When the solution was let to stand two phases of liquid were created. However, after remixing the solution became homogenous. After adding tattoo ink to the solution, the tattooing composition is ready for injection.

Exemplary composition for tattooing No. 12

[0088] An exemplary composition for tattooing No. 12 comprises: 0.1013 gr CBD

0.0507 gr CBG

0.1028 gr CBN

0.6351 gr olive oil

0.0562 gr soybean lecithin

The mixture was infused at substantially 90°C for substantially 110 minutes.

The mixture was briefly vortexed.

0.2215 gr Tapioca maltodextrin N-Zorbit M was added to the mixture, and the mixture was mixed until it became solid.

The solid mixture was dissolved in 3.9550 gr tattoo ink.

Exemplary method No. 1 for preparing a composition for tattooing

[0089] An exemplary method No. 1 for preparing a composition for tattooing comprises: obtaining 0.1 gr THCA (cannabinoid); decarboxylating the THCA by heating at substantially 104°C for substantially 90 minutes, to form decarboxylated THCA (THC); mixing the decarboxylated THCA (THC) with 0.24 gr olive oil (oil as a delivery agent) and 0.02 gr soybean lecithin (emulsifier as a delivery agent) for obtaining a mixture of THCA, olive oil and soybean lecithin; infusing the mixture of THCA, olive oil and soybean lecithin by heating at substantially 90°C for substantially 90 minutes; adding to the mixture 0.02 gr myrcene (terpene), 0.02 gr linalool (terpene), 0.02 gr terpinolene (terpene) and 0.02 gr caryophyllene (terpene), and mixing; adding to the mixture 5.73 gr black tattoo ink (colored substance), and mixing.

A total amount of 6.89 gr composition for tattooing was obtained.

[0090] In another version of method No. 1 for preparing a composition for tattooing, Tapioca maltodextrin N-Zorbit M was added to the composition before adding the tattoo ink. After adding the Tapioca maltodextrin N-Zorbit M, and before adding the tattoo ink, the mixture was mixed until the texture of the mixture changed from oily liquid to paste. After further mixing the mixture transformed to powder. Then, the paste, or the powder (both forms where appropriate), was diluted with the tattoo ink and was ready for injection. Exemplary method No. 2 for preparing a composition for tattooing

[0091] An exemplary method No. 2 for preparing a composition for tattooing comprises: obtaining a cannabinoid; mixing the cannabinoid with oil and lecithin;

[0092] infusing the mixture of cannabinoid, oil and lecithin by heating at substantially 90°C for substantially 90 minutes; further mixing with a terpene; further mixing with maltodextrin, preferably Tapioca maltodextrin N-Zorbit M, until a mixture in a form of paste, or powder after further mixing, is formed; and mixing the paste, or powder, with a colored substance.

[0093] The embodiments and examples described above relate to a composition for tattooing that is ready-to-use, namely ready for tattooing injection, as the composition for tattooing comprises a tattoo ink.

RELIEF ADDITIVE FOR TATTOOING

[0094] The present subject matter additionally provides a relief additive for tattooing that comprises the components of the composition for tattooing, according to the various embodiments described above, except the tattoo ink. The relief additive for tattooing is configured to be mixed with a tattoo ink and used for tattooing. In other words, mixing the relief additive for tattooing with a tattoo ink gives rise to a composition for tattooing, as described herein. It should be mentioned that for the sake of simplicity only, the term “relief additive to tattooing” will be occasionally referred to hereinafter as “the additive”, or “the relief additive”.

[0095] According to one embodiment, the relief additive is liquid. According to another embodiment, the relief additive is a paste, or ointment. According to yet another embodiment, the relief additive is solid.

[0096] An advantage of the additive is that the additive allows the user to determine himself the amount of the cannabinoids, or terpenes, or cannabinoids and terpenes that are added to a tattoo ink, by choosing the amount of the additive that is added to the tattoo ink by the user. This is advantageous over the composition for tattooing as described herein, which is manufactured in certain concentrations of the cannabinoids, or terpenes, or cannabinoids and terpenes, and other components that are pre-determined by the manufacture. This embodiment increases the flexibility of the user in terms of the concentration of the cannabinoids, or terpenes, or cannabinoids and terpenes, and the additional components in the mixture of the additive and the tattoo ink.

[0097] Another advantage of the additive is that the additive provides the user the freedom to choose any type, or color, of tattoo ink that is to be mixed with the additive. This is advantageous over the composition for tattooing as described herein, which is manufactured a priori with tattoo inks of certain types and of certain colors that are pre-determined by the manufacturer.

[0098] An advantage of the solid additive over the composition for tattooing is that the shelflife of the solid additive is much higher than the shelf-life of the composition for tattooing. This is due to the solid phase nature and dryness of the additive that allow the additive to be kept for long periods, compared to the liquid composition for tattooing.

[0099] Any type and texture of the solid additive is under the scope of the present subject matter. For example, the solid additive can be in a form of a powder, and the powder can be in any form, for example in a form of a coarse-grained powder, a fine-grained powder, a nanopowder, and the like, according to the size of the grains of the powder. Preferably, the powder is a nano-powder. The term “nano-powder” as disclosed herein refers to a powder comprising nano-particles. The term “nano-particle” as disclosed herein refers to a particle that is in a size of less than substantially 100 nm. An advantage of the nano-powder is greater homogeneity of the nano-powder compared to the homogeneity of a powder comprising larger particles. Another advantage of the additive in a form of nano-powder, paste, ointment, or liquid, is greater homogeneity after mixing each one of them with tattoo ink, compared to the homogeneity of a powdered additive comprising particles larger than nano-particles that is mixed with the tattoo ink. Another advantage of the additive in a form of nano-powder, paste, ointment, or liquid, after mixing with a tattoo ink, is improved absorption of active ingredients, like cannabinoids, or terpenes, or both cannabinoids and terpenes, and the like, during injection of the mixture of tattoo ink with the aforementioned forms of the additive, compared to a mixture of tattoo ink with an additive in a form of a powder comprising particles that are larger than nano-particles. [00100] Another exemplary form of the additive that is under the scope of the present subject matter, is a capsule. In this example, the powder is contained in a capsule of any type. The additive that is contained in the capsule can be solid, a paste, an ointment, or a liquid. Yet another exemplary form of the additive is a pill, which can be manufactured by compressing the powder, and optionally encapsulating the compressed powder.

[00101] Any method for preparing the relief additive for tattooing is under the scope of the present subject matter. Some exemplary methods and examples for preparing the relief additive, and mixing the relief additive with tattoo ink are described hereinafter.

[00102] The present subject matter further provides a method for preparing a liquid relief additive for tattooing, the method comprising: providing a relief agent; heating the relief agent at a first temperature for a first period of time; adding to the relief agent at least one emulsifier, at least one delivery agent and water to provide a mixture; mixing the mixture while heating at a second temperature for a second period of time; and sonicating the mixture to provide a liquid relief additive.

[00103] The present subject matter further provides a method for preparing a relief additive for tattooing in a form of paste or ointment, the method comprising: providing a relief agent; heating the relief agent at a first temperature for a first period of time; adding to the relief agent at least one emulsifier, at least one delivery agent and water to provide a mixture; mixing the mixture while heating at a second temperature for a second period of time; sonicating the mixture; and evaporating the mixture to provide a relief additive in a form of a paste, or ointment.

[00104] The present subject matter further provides a method for preparing a solid relief additive for tattooing, the method comprising: providing a relief agent; heating the relief agent at a first temperature for a first period of time; adding to the relief agent at least one emulsifier, at least one delivery agent and water to provide a mixture; mixing the mixture while heating at a second temperature for a second period of time; sonicating the mixture; evaporating the mixture, and drying the mixture to provide a solid relief additive for tattooing.

[00105] According to one embodiment, the relief agent is at least one cannabinoid. According to another embodiment, the relief agent is at least one terpene. According to yet another embodiment, the relief agent is a combination of at least one cannabinoid and at least one terpene. It should be noted that the embodiment of relief agent pertains also to the composition for tattooing described above.

[00106] According to one embodiment, any type of cannabinoid, terpene, emulsifier and delivery agent is under the scope of the present subject matter, particularly as described herein.

[00107] According to one embodiment, when at least one of the at least one cannabinoid is in an acidic form, for example THCA, the at least one cannabinoid that is in an acidic form is decarboxylated before adding to the mixture. Embodiments of the decarboxylation process are described above.

[00108] According to one embodiment, the first temperature is substantially 45°C.

[00109] According to one embodiment, the first period of time is substantially 5 to 10 minutes.

[00110] According to another embodiment, the first period of time is substantially 6 minutes.

[00111] According to one embodiment, the adding to the relief agent at least one emulsifier, at least one delivery agent and water, further comprising adding to the relief agent at least one humectant, known as a substance that absorbs water. In some experiments preformed by the inventor it was found that the addition of the at least one humectant improves the homogeneity of the relief additive for tattooing. Any type of humectant is under the scope of the present subject matter, for example, birch xylitol, xylitol and the like. According to one embodiment, the at least one humectant is birch xylitol and xylitol.

[00112] According to one embodiment, the second temperature is in a range of substantially 50-65°C.

[00113] According to another embodiment, the second temperature does not exceed substantially 65°C.

[00114] According to yet another embodiment, the second temperature is substantially 62°C.

[00115] According to one embodiment, the second period of time is in a range of substantially 5-10 minutes.

[00116] According to another embodiment, the second period of time is substantially 10 minutes.

[00117] According to one embodiment, the sonicating the mixture comprising at least one round of sonication, the round of sonication comprising: sonication for a third period of time at a third temperature; cooling to a fourth temperature while mixing the mixture.

[00118] According to another embodiment, the sonicating the mixture comprising one round of sonication.

[00119] According to yet another embodiment, the sonicating the mixture comprising multiple rounds of sonication.

[00120] According to still another embodiment, the sonicating the mixture comprising three rounds of sonication.

[00121] According to a further embodiment, the third period of time is up to substantially 3 minutes.

[00122] According to yet a further embodiment, the third period of time is substantially 3 minutes. [00123] According to still a further embodiment, the third temperature does not exceed substantially 70°C.

[00124] According to an additional embodiment, the fourth temperature is in a range of substantially 25-35°C.

[00125] According to yet an additional embodiment, the fourth temperature is substantially 35°C.

[00126] According to still an additional embodiment, the fourth temperature is ambient temperature.

[00127] According to one embodiment, the method further comprising filtering the mixture, before evaporating the mixture.

[00128] Any method for evaporating the mixture is under the scope of the present subject matter.

[00129] According to one embodiment, the evaporating the mixture is under vacuum.

[00130] Any method for drying the mixture is under the scope of the present subject matter, for example heating, spray drying, freeze-drying (lyophilizing) and the like.

[00131] The present subject matter additionally provides a method for preparing a tattoo ink comprising a relief agent, the method comprising: mixing a relief additive for tattooing with tattoo ink.

[00132] According to one embodiment, the relief additive is liquid.

[00133] According to another embodiment, the relief additive is in a form of a paste, or ointment.

[00134] According to yet another embodiment, the relief additive is solid.

[00135] It is appreciated that certain features of the subject matter, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the subject matter, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable sub combination.

[00136] Although the subject matter has been described in conjunction with specific embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art. Accordingly, it is intended to embrace all such alternatives, modifications and variations that fall within the spirit and broad scope of the appended claims.

Claims

CLAIMS A composition for tattooing, the composition comprising: a colored substance; and a cannabinoid. The composition for tattooing according to claim 1, wherein the cannabinoid is in a lowest concentration that has an effect on a patient. The composition for tattooing according to claim 1 or 2, further comprising a delivery agent. The composition for tattooing according to claim 3, wherein the delivery agent is an oil. The composition for tattooing according to claim 3, wherein the delivery agent is an emulsifier. The composition for tattooing according to claim 3, wherein the delivery agent is a mixture of an oil and an emulsifier. The composition for tattooing according to any one of claims 1 to 6, further comprising a terpene. The composition for tattooing according to any one of claims 1 to 7, further comprising water. The composition for tattooing according to any one of claims 1 to 8, further comprising maltodextrin. A method for preparing a composition for tattooing, the method comprising: mixing a cannabinoid and colored substance. The method for preparing a composition for tattooing according to claim 10, further comprising mixing with a delivery agent. The method for preparing a composition for tattooing according to claim 11, wherein the delivery agent is an oil. The method for preparing a composition for tattooing according to claim 11, wherein the delivery agent is an emulsifier. The method for preparing a composition for tattooing according to claim 11, wherein the delivery agent is a mixture of an oil and an emulsifier. The method for preparing a composition for tattooing according to claim 14, further comprising infusing the cannabinoid with the oil and the emulsifier. The method for preparing a composition for tattooing according to claim 15, wherein the infusing comprising heating of the mixture of the cannabinoid, the oil and the emulsifier. The method for preparing a composition for tattooing according any one of claims 10 to

16, further comprising mixing with a terpene. The method for preparing a composition for tattooing according to any one of claims 10 to

17, further comprising mixing with maltodextrin. The method for preparing a composition for tattooing according to any one of claims 10 to

18, further comprising mixing with a flavonoid. The method for preparing a composition for tattooing according to any one of claims 10 to

19, further comprising decarboxylating the cannabinoid, when the cannabinoid is in an acidic form. The method for preparing a composition for tattooing according to 20, the decarboxylating the cannabinoid comprises heating the cannabinoid. A relief additive for tattooing as herein described. A method for preparing a relief additive for tattooing as herein described. A method for preparing a tattoo ink comprising a relief agent as herein described.