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WO2024096835A1 - Préparations de soins buccaux comprenant une combinaison d'huiles essentielles normalisées et d'agents antiseptiques - Google Patents

Préparations de soins buccaux comprenant une combinaison d'huiles essentielles normalisées et d'agents antiseptiques Download PDF

Info

Publication number
WO2024096835A1
WO2024096835A1 PCT/TR2023/050929 TR2023050929W WO2024096835A1 WO 2024096835 A1 WO2024096835 A1 WO 2024096835A1 TR 2023050929 W TR2023050929 W TR 2023050929W WO 2024096835 A1 WO2024096835 A1 WO 2024096835A1
Authority
WO
WIPO (PCT)
Prior art keywords
standardized
chlorhexidine
essential oils
oral formulation
antimicrobial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/TR2023/050929
Other languages
English (en)
Inventor
Ayse Esra KARADAG
Sevde Nur BILTEKIN KALELI
Ayşegül ÇAŞKURLU
İbrahim Hazar KOCAOLUK
Salih Mete ARMUTLU
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Istanbul Medipol Universitesi
Original Assignee
Istanbul Medipol Universitesi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from TR2022/016513 external-priority patent/TR2022016513A2/tr
Application filed by Istanbul Medipol Universitesi filed Critical Istanbul Medipol Universitesi
Publication of WO2024096835A1 publication Critical patent/WO2024096835A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/43—Guanidines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9755—Gymnosperms [Coniferophyta]
    • A61K8/9761—Cupressaceae [Cypress family], e.g. juniper or cypress
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses

Definitions

  • the present disclosure relates to the evaluation of the in-vitro antimicrobial effects of a triple essential oil mixture of standardized Mentha piperita, Thymus serpyllum, and Pelargonium graveolens or specifically designed formulations containing combinations of Juniperus communis or Zingiber officinalis or Pistacia lentiscus essential oils with chlorhexidine against dental caries pathogens.
  • Dental infections such as dental caries and periodontal diseases, are among the most common bacterial infections in humans.
  • Streptococcus mutans is found in biofilms, which are formed on tooth surfaces and are also known as dental plaque. They live in the oral cavity and form dental plaque.
  • S. mutans increases the acidity of the oral environment by producing organic acids from food residues, and as a result, it has a mineralizing effect on tooth structure.
  • Many antimicrobial agents are available to inhibit biofilm formation and bacterial tooth mineralization, but these agents may exhibit side effects such as fluorosis, chromatism, taste disturbance, burning sensation, ulcerative lesions, and enamel erosion.
  • communis (common juniper) is a genus that spreads naturally in Tiirkiye and has different uses ethnobotanically such as urine enhancer, diaphoretic, gastric, antiseptic, emmenagogue, anti-inflammatory, hemorrhagic inhibitor.
  • Z. officinalis is a tuber-rooted plant that grows under the ground. It is used among people to help digestion, relieve nausea, regulate bleeding, reduce rheumatism, regulate respiration, to prevent clotting and cholesterol.
  • P. lentiscus (gum tree) grows in various places in Tiirkiye. It has been used ethnobotanically for many years as a treatment for rabies, stomach, intestinal, lung, and various dental conditions.
  • a triple essential oil mixture consisting of Mentha piperita, Thymus serpyllum and Pelargonium graveolens or any of the essential oils Juniperus communis, Zingiber officinalis, Pistacia lentiscus were combined with different concentrations of the chlorhexidine substance frequently used in oral care on the market, and as a result, antimicrobial essential oil-chlorhexidine combinations were obtained. Accordingly, it is aimed to prepare a safe, innovative, and naturally sourced oral care preparation that requires less chemical use by the inventors.
  • the displayed invention intends to standardize the essential oils selected in line with their use among the public and experimental study results by gas chromatography over major components/markers, to examine the antimicrobial activities and synergistic effects of the selected essential oils both in-house and with chlorhexidine by in vitro checkerboard method, and to design the pharmaceutical formulation suitable for oral use from the effective combined doses determined as a result of the activity studies of the standardized oils.
  • the main object of the invention which is to prepare a safe, innovative and naturally sourced oral care preparation that requires less chemical use
  • the standardization process of the supplied oils is based on the given literature and the major substances given in the European Pharmacopoeia.
  • the standardization process is presented in detail with methods such as diode array detector, mass spectrometer, etc.
  • the analysis of the oils is illuminated by simultaneous analyses of the GC and GC/MS systems.
  • GC analysis is performed with the Agilent 6890N GC system.
  • the analysis is performed using the Innowax FSC column (60 m x 0.25 mm, film thickness 0.25 pm) and Helium (0.8 mL/min) as carrier gas.
  • the GC oven temperature is increased to 220°C with an increase of 4°C/min at 60°C for 10 minutes, kept for 10 minutes, and then increased to 240°C with an increase of l°C/min.
  • the FID detector temperature is set to 300°C.
  • the relative ratios of the compounds are calculated using the FID chromatogram computer.
  • the volatile compounds separated in the GC column were then identified by taking individual mass spectra of each that act as detectors. Volatile compounds are separated into GC columns and then identified by taking individual mass spectra in mass spectrometry. Here, the mass spectrum is used in the detector function.
  • GC/MS analysis is performed by using the Agilent 5975 GC/MSD system.
  • Innowax FSC column (60 m x 0.25 mm, film thickness 0.25 pm) is used for separation of components.
  • Helium 0.8 mL/min
  • the GC oven temperature is increased to 220°C with an increase of 4°C/min at 60°C for 10 minutes, kept for 10 minutes, and then increased to 240°C with an increase of l°C/min.
  • Split applied at a rate of 1 in 40 and the energy of the mass spectra is taken as 70 eV.
  • Mass spectra are taken with an energy of 70 eV. Mass values are recorded between m/z 35 and 450.
  • the adhesion indices (RRI) of the substances in the column are calculated with reference to n-Alkanes. Component analyses were carried out using "Ba ⁇ er Essential Oil Components Library", Wiley, and MassFinder 3.1 Library Scanning Software.
  • the formulations contain 1-5% volatile oils by weight.
  • the essential oils were weighed and dissolved in ethanol; sodium chloride and sodium bicarbonate were gradually added using a mechanical stirrer (500 rpm, 30 minutes), respectively.
  • the formulation was filtered, and the volume of filtrate was made up to 10 mL using distilled water.
  • Essential oils were found to be effective against dental caries microorganisms in the concentration range of 0.01-0.5 mg/mL.
  • chlorhexidine essential oil was combined separately and the chlorhexidine: essential oil ratios were determined in the range of 0.001- 0.004: 1-0.25 mg/mL.
  • chlorhexidine In single combinations with chlorhexidine, combinations such as Pistacic . chlorhexidine (0.25:0.001 mg/ml), Juniperus communis', chlorhexidine (0.0125:0.001 mg/ml), Zingiber officinalis', chlorhexidine (0.5:0.001 mg/ml) found to be effective.
  • the aim is to evaluate the efficacy, toxicity, and stability of the designed formulations and to achieve a combination of essential oils with high antimicrobial activity and a synergistic effect.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

La présente divulgation concerne l'évaluation des effets antimicrobiens in vitro de mélanges d'huiles essentielles triples normalisées de Mentha piperita, Thymus serpyllum et Pelargonium graveolens ou des formulations plus particulièrement conçues contenant des combinaisons d'huiles essentielles de Juniperus communis ou Zingiber officinalis ou Pistacia lentiscus avec de la chlorhexidine contre des agents pathogènes de caries dentaires.
PCT/TR2023/050929 2022-11-01 2023-09-07 Préparations de soins buccaux comprenant une combinaison d'huiles essentielles normalisées et d'agents antiseptiques Ceased WO2024096835A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
TR2022/016513 TR2022016513A2 (tr) 2022-11-01 Mentha piperita & Thymus serpyllum & Pelargonium graveolens Uçucu Yağlarını İçeren Ağız Bakım Preparatları
TR2022016513 2022-11-01
TR2022/016569 TR2022016569A2 (tr) 2022-11-02 Juniperus communis & Zingiber officinalis & Pistacia lentiscus Uçucu Yağlarını İçeren Ağız Bakım Preparatları
TR2022016569 2022-11-02

Publications (1)

Publication Number Publication Date
WO2024096835A1 true WO2024096835A1 (fr) 2024-05-10

Family

ID=90931209

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/TR2023/050929 Ceased WO2024096835A1 (fr) 2022-11-01 2023-09-07 Préparations de soins buccaux comprenant une combinaison d'huiles essentielles normalisées et d'agents antiseptiques

Country Status (1)

Country Link
WO (1) WO2024096835A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6264926B1 (en) * 1999-02-12 2001-07-24 Council Of Scientific And Industrial Research Formulation useful as a natural herbal tooth powder
JP2008143870A (ja) * 2006-12-13 2008-06-26 Lion Corp 口腔用組成物
WO2011068811A1 (fr) * 2009-12-04 2011-06-09 Colgate-Palmolive Company Compositions orales contenant des extraits de zingiber officinale et procedes associes
WO2014203263A1 (fr) * 2013-06-18 2014-12-24 Colgate-Palmolive Company Compositions antimicrobiennes comprenant des combinaisons d'huiles essentielles

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6264926B1 (en) * 1999-02-12 2001-07-24 Council Of Scientific And Industrial Research Formulation useful as a natural herbal tooth powder
JP2008143870A (ja) * 2006-12-13 2008-06-26 Lion Corp 口腔用組成物
WO2011068811A1 (fr) * 2009-12-04 2011-06-09 Colgate-Palmolive Company Compositions orales contenant des extraits de zingiber officinale et procedes associes
WO2014203263A1 (fr) * 2013-06-18 2014-12-24 Colgate-Palmolive Company Compositions antimicrobiennes comprenant des combinaisons d'huiles essentielles

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SABZGHABAEE ALI MOHAMMAD, SHIRDARE ZAHRA; EBADIAN BEHNAZ; ASLANI ABOLFAZL; GHANNADI ALIREZA; : "Clinical evaluation of the essential oil of Pelargonium graveolens for the treatment of denture stomatitis", DENTAL RESEARCH JOURNAL, 1 January 2011 (2011-01-01), XP093171079, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556280/pdf/DRJ-8-105.pdf> *

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