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WO2024080892A3 - Perfusion filtration system for continuous cultivation of cell cultures - Google Patents

Perfusion filtration system for continuous cultivation of cell cultures Download PDF

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Publication number
WO2024080892A3
WO2024080892A3 PCT/RU2023/000301 RU2023000301W WO2024080892A3 WO 2024080892 A3 WO2024080892 A3 WO 2024080892A3 RU 2023000301 W RU2023000301 W RU 2023000301W WO 2024080892 A3 WO2024080892 A3 WO 2024080892A3
Authority
WO
WIPO (PCT)
Prior art keywords
ability
automated control
valves
cell cultures
control system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/RU2023/000301
Other languages
French (fr)
Other versions
WO2024080892A2 (en
Inventor
Artem Evgenevich NIKULIN
Nikolay Nikolaevich POPLAVKA
Dmitriy Vyacheslavovich MAKAROV
Maksim Sergeevich BENEVOLENSKIY
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
"biotechno" LLC
Original Assignee
"biotechno" LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from RU2022126480A external-priority patent/RU2800874C1/en
Application filed by "biotechno" LLC filed Critical "biotechno" LLC
Publication of WO2024080892A2 publication Critical patent/WO2024080892A2/en
Publication of WO2024080892A3 publication Critical patent/WO2024080892A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/16—Hollow fibers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a perfusion filtration system for continuous cultivation of cell cultures, including a pump (1) for supplying circulating liquid to the system; a filter holder with replaceable filter elements (2); an automated control system of membrane valves with software, which consists of four separate filtrate withdrawal lines with pressure sensors (3), which allow recording the pressure changes and the rate of pressure change, which indicate the degree of contamination of the filter element, wherein the automated control system has the ability to regulate the frequency of opening and closing of membrane valves (4.1-7.3) and start the self- cleaning mode of the filter elements in automatic mode, where there are three membrane valves on each filtrate withdrawal line, and the valves (4.1; 5.1; 6.1; 7.1) and (4.3; 5.3; 6.3; 7.3) operate with the ability to ensure flow is cut off, and the valves (4.2; 5.2; 6.2; 7.2) operate with the ability to ensure the creation of flow, and wherein the automated control system has the ability to ensure cleaning of filter elements in automatic mode with automated control of filtrate withdrawal with replacement of the culture medium in the bioreactor to the fresh culture medium. The technical result consists in increasing the density cell cultures and in increasing the product yield.
PCT/RU2023/000301 2022-10-11 2023-10-11 Perfusion filtration system for continuous cultivation of cell cultures Ceased WO2024080892A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2022126480 2022-10-11
RU2022126480A RU2800874C1 (en) 2022-10-11 Perfusion filtration method of continuous culturing of cell cultures

Publications (2)

Publication Number Publication Date
WO2024080892A2 WO2024080892A2 (en) 2024-04-18
WO2024080892A3 true WO2024080892A3 (en) 2024-10-17

Family

ID=89222874

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/RU2023/000301 Ceased WO2024080892A2 (en) 2022-10-11 2023-10-11 Perfusion filtration system for continuous cultivation of cell cultures

Country Status (1)

Country Link
WO (1) WO2024080892A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012086720A1 (en) * 2010-12-22 2012-06-28 東レ株式会社 Method for producing chemical by continuous fermentation
WO2017029305A1 (en) * 2015-08-20 2017-02-23 Ge Healthcare Bio-Sciences Ab Re-circulation loop in cff/tff single use flow path
US20210261900A1 (en) * 2018-08-29 2021-08-26 Cytiva Sweden Ab System for Filtration and Associated Method Thereof
US20220073861A1 (en) * 2018-05-04 2022-03-10 Genzyme Corporation Perfusion Bioreactor With Filtration Systems

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9365876B2 (en) 2010-12-09 2016-06-14 Toray Industries, Inc. Method for producing chemical by continuous fermentation

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012086720A1 (en) * 2010-12-22 2012-06-28 東レ株式会社 Method for producing chemical by continuous fermentation
WO2017029305A1 (en) * 2015-08-20 2017-02-23 Ge Healthcare Bio-Sciences Ab Re-circulation loop in cff/tff single use flow path
US20220073861A1 (en) * 2018-05-04 2022-03-10 Genzyme Corporation Perfusion Bioreactor With Filtration Systems
US20210261900A1 (en) * 2018-08-29 2021-08-26 Cytiva Sweden Ab System for Filtration and Associated Method Thereof

Also Published As

Publication number Publication date
WO2024080892A2 (en) 2024-04-18

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