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WO2024059581A3 - Engineered dna polymerase variants - Google Patents

Engineered dna polymerase variants Download PDF

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Publication number
WO2024059581A3
WO2024059581A3 PCT/US2023/073997 US2023073997W WO2024059581A3 WO 2024059581 A3 WO2024059581 A3 WO 2024059581A3 US 2023073997 W US2023073997 W US 2023073997W WO 2024059581 A3 WO2024059581 A3 WO 2024059581A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna polymerase
engineered dna
polymerase variants
polymerase polypeptides
compositions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2023/073997
Other languages
French (fr)
Other versions
WO2024059581A2 (en
Inventor
Jason FELL
Sandy M. GOMES
Supriya Vijaykumar KADAM
Anders Matthew KNIGHT
Larson Lyle MATZDORFF
Mathew G. MILLER
Zhe RUI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Codexis Inc
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Priority to CN202380065381.9A priority Critical patent/CN119923465A/en
Priority to EP23866399.1A priority patent/EP4587562A2/en
Publication of WO2024059581A2 publication Critical patent/WO2024059581A2/en
Publication of WO2024059581A3 publication Critical patent/WO2024059581A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present disclosure relates to engineered DNA polymerase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered DNA polymerase polypeptides. The present disclosure also provides methods of using the engineered DNA polymerase polypeptides or compositions thereof for diagnostic and other purposes.
PCT/US2023/073997 2022-09-12 2023-09-12 Engineered dna polymerase variants Ceased WO2024059581A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202380065381.9A CN119923465A (en) 2022-09-12 2023-09-12 Engineered DNA polymerase variants
EP23866399.1A EP4587562A2 (en) 2022-09-12 2023-09-12 Engineered dna polymerase variants

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US202263375371P 2022-09-12 2022-09-12
US63/375,371 2022-09-12
US202363494211P 2023-04-04 2023-04-04
US63/494,211 2023-04-04

Publications (2)

Publication Number Publication Date
WO2024059581A2 WO2024059581A2 (en) 2024-03-21
WO2024059581A3 true WO2024059581A3 (en) 2024-05-10

Family

ID=90275809

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/073997 Ceased WO2024059581A2 (en) 2022-09-12 2023-09-12 Engineered dna polymerase variants

Country Status (3)

Country Link
EP (1) EP4587562A2 (en)
CN (1) CN119923465A (en)
WO (1) WO2024059581A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210309977A1 (en) * 2018-10-29 2021-10-07 Codexis, Inc. Engineered dna polymerase variants

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210309977A1 (en) * 2018-10-29 2021-10-07 Codexis, Inc. Engineered dna polymerase variants

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE UNIPROTKB [online] 11 June 2014 (2014-06-11), ANONYMOUS: "A0A023CMU9 · A0A023CMU9_9BACI", XP093171432, Database accession no. A0A023CMU9 *

Also Published As

Publication number Publication date
WO2024059581A2 (en) 2024-03-21
EP4587562A2 (en) 2025-07-23
CN119923465A (en) 2025-05-02

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