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WO2024044739A3 - Compositions and methods for glycorna imaging via proximity ligation assay - Google Patents

Compositions and methods for glycorna imaging via proximity ligation assay Download PDF

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Publication number
WO2024044739A3
WO2024044739A3 PCT/US2023/072898 US2023072898W WO2024044739A3 WO 2024044739 A3 WO2024044739 A3 WO 2024044739A3 US 2023072898 W US2023072898 W US 2023072898W WO 2024044739 A3 WO2024044739 A3 WO 2024044739A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
methods
glycorna
ligation assay
proximity ligation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2023/072898
Other languages
French (fr)
Other versions
WO2024044739A2 (en
Inventor
Yi Lu
Yuan Ma
Weijie GUO
Quanbing MOU
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Texas System
University of Texas at Austin
Original Assignee
University of Texas System
University of Texas at Austin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Texas System, University of Texas at Austin filed Critical University of Texas System
Publication of WO2024044739A2 publication Critical patent/WO2024044739A2/en
Publication of WO2024044739A3 publication Critical patent/WO2024044739A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/02—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates involving antibodies to sugar part of glycoproteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cellular glycans play important roles in biology by regulating many essential functions, including cell communications, homeostasis, immunomodulation, and embryogenesis. While proteoglycans, glycoproteins, and glycosphingolipids have been widely studied, glycosylated RNAs (glycoRNAs) have recently been discovered on the surfaces of multiple cell types, organisms, and species. The present invention describes compositions for use in a proximity assay for detecting glycosylated molecules in a sample, and methods of use of the compositions for a proximity assay for detecting glycosylated molecules.
PCT/US2023/072898 2022-08-26 2023-08-25 Compositions and methods for glycorna imaging via proximity ligation assay Ceased WO2024044739A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263373605P 2022-08-26 2022-08-26
US63/373,605 2022-08-26

Publications (2)

Publication Number Publication Date
WO2024044739A2 WO2024044739A2 (en) 2024-02-29
WO2024044739A3 true WO2024044739A3 (en) 2024-07-18

Family

ID=90014136

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/072898 Ceased WO2024044739A2 (en) 2022-08-26 2023-08-25 Compositions and methods for glycorna imaging via proximity ligation assay

Country Status (1)

Country Link
WO (1) WO2024044739A2 (en)

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000253883A (en) * 1999-03-10 2000-09-19 Nitto Denko Corp Aptamers that specifically recognize chitin
US7250556B2 (en) * 2002-05-01 2007-07-31 University Of Georgia Research Foundation, Inc. Transposable elements in rice and methods of use
US20080293051A1 (en) * 2005-08-30 2008-11-27 Board Of Regents, The University Of Texas System proximity ligation assay
US20110288159A1 (en) * 2008-04-03 2011-11-24 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Methods of expressing a polypeptide in the brain and nucleic acid constructs capable of same
US20130323729A1 (en) * 2010-10-29 2013-12-05 Olink Ab Proximity Ligation Technology for Western Blot Applications
US20140087957A1 (en) * 2012-09-25 2014-03-27 Marko Vuskovic Methods, assays and kits for cancer diagnosis and screening utilizing glycan-binding and glycan epitopes
US10465235B2 (en) * 2011-05-24 2019-11-05 Navinci Diagnostics Ab Multiplexed proximity ligation assay
US20200178540A1 (en) * 2018-12-07 2020-06-11 Institut National De La Recherche Scientifique Biological Control Agents Against Fire Blight
US20200236914A1 (en) * 2018-04-27 2020-07-30 The Curators Of The University Of Missouri Pathogen-resistant animals having modified aminopeptidase n (anpep) genes
WO2020180645A1 (en) * 2019-03-01 2020-09-10 Meso Scale Technologies, Llc. Electrochemiluminescent labeled probes for use in immunoassay methods, methods using such and kits comprising same
WO2020212541A1 (en) * 2019-04-16 2020-10-22 University Of Nottingham Fungal strains, production and uses thereof
US11041850B2 (en) * 2015-07-07 2021-06-22 The Regents Of The University Of California Method for detecting protein-specific glycosylation

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000253883A (en) * 1999-03-10 2000-09-19 Nitto Denko Corp Aptamers that specifically recognize chitin
US7250556B2 (en) * 2002-05-01 2007-07-31 University Of Georgia Research Foundation, Inc. Transposable elements in rice and methods of use
US20080293051A1 (en) * 2005-08-30 2008-11-27 Board Of Regents, The University Of Texas System proximity ligation assay
US20110288159A1 (en) * 2008-04-03 2011-11-24 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Methods of expressing a polypeptide in the brain and nucleic acid constructs capable of same
US20130323729A1 (en) * 2010-10-29 2013-12-05 Olink Ab Proximity Ligation Technology for Western Blot Applications
US10465235B2 (en) * 2011-05-24 2019-11-05 Navinci Diagnostics Ab Multiplexed proximity ligation assay
US20140087957A1 (en) * 2012-09-25 2014-03-27 Marko Vuskovic Methods, assays and kits for cancer diagnosis and screening utilizing glycan-binding and glycan epitopes
US11041850B2 (en) * 2015-07-07 2021-06-22 The Regents Of The University Of California Method for detecting protein-specific glycosylation
US20200236914A1 (en) * 2018-04-27 2020-07-30 The Curators Of The University Of Missouri Pathogen-resistant animals having modified aminopeptidase n (anpep) genes
US20200178540A1 (en) * 2018-12-07 2020-06-11 Institut National De La Recherche Scientifique Biological Control Agents Against Fire Blight
WO2020180645A1 (en) * 2019-03-01 2020-09-10 Meso Scale Technologies, Llc. Electrochemiluminescent labeled probes for use in immunoassay methods, methods using such and kits comprising same
WO2020212541A1 (en) * 2019-04-16 2020-10-22 University Of Nottingham Fungal strains, production and uses thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FLYNN RYAN A.; PEDRAM KAYVON; MALAKER STACY A.; BATISTA PEDRO J.; SMITH BENJAMIN A.H.; JOHNSON ALEX G.; GEORGE BENSON M.; MAJZOUB : "Small RNAs are modified with N-glycans and displayed on the surface of living cells", CELL, ELSEVIER, AMSTERDAM NL, vol. 184, no. 12, 17 May 2021 (2021-05-17), Amsterdam NL , pages 3109, XP086614102, ISSN: 0092-8674, DOI: 10.1016/j.cell.2021.04.023 *
JALILI ROXANA, HORECKA JOE, SWARTZ JAMES R., DAVIS RONALD W., PERSSON HENRIK H. J.: "Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 115, no. 5, 30 January 2018 (2018-01-30), pages E925 - E933, XP055800525, ISSN: 0027-8424, DOI: 10.1073/pnas.1718283115 *
LI QINYING, LI GUOYUN, ZHOU YU, ZHANG XIN, SUN MEI, JIANG HAO, YU GUANGLI: "Comprehensive N -Glycome Profiling of Cells and Tissues for Breast Cancer Diagnosis", JOURNAL OF PROTEOME RESEARCH, AMERICAN CHEMICAL SOCIETY, vol. 18, no. 6, 7 June 2019 (2019-06-07), pages 2559 - 2570, XP093196282, ISSN: 1535-3893, DOI: 10.1021/acs.jproteome.9b00073 *
MA YUAN, GUO WEIJIE, MOU QUANBING, SHAO XIANGLI, LYU MINGKUAN, GARCIA VALERIA, KONG LINGGEN, LEWIS WHITNEY, WARD CARSON, YANG ZHEN: "Spatial imaging of glycoRNA in single cells with ARPLA", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 42, no. 4, 1 April 2024 (2024-04-01), New York, pages 608 - 616, XP093196286, ISSN: 1087-0156, DOI: 10.1038/s41587-023-01801-z *

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