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WO2024044759A3 - Methods of using exonucleases to make tiny rnas on argonaute proteins - Google Patents

Methods of using exonucleases to make tiny rnas on argonaute proteins Download PDF

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Publication number
WO2024044759A3
WO2024044759A3 PCT/US2023/072930 US2023072930W WO2024044759A3 WO 2024044759 A3 WO2024044759 A3 WO 2024044759A3 US 2023072930 W US2023072930 W US 2023072930W WO 2024044759 A3 WO2024044759 A3 WO 2024044759A3
Authority
WO
WIPO (PCT)
Prior art keywords
exonuclease
exonucleases
methods
argonaute proteins
make tiny
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2023/072930
Other languages
French (fr)
Other versions
WO2024044759A2 (en
Inventor
Kotaro NAKANISHI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ohio State Innovation Foundation
Original Assignee
Ohio State Innovation Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ohio State Innovation Foundation filed Critical Ohio State Innovation Foundation
Publication of WO2024044759A2 publication Critical patent/WO2024044759A2/en
Publication of WO2024044759A3 publication Critical patent/WO2024044759A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/26—Endoribonucleases producing 5'-phosphomonoesters (3.1.26)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present disclosure provides a system for trimming, cleaving, or cutting a target nucleic acid, utilizing a nucleic acid trimming system comprising a non-coding ribonucleic acid (non-coding RNA) and an exonuclease enzyme, the exonuclease comprising interferon-stimulated gene 20 kDa (ISG20), three prime repair exonuclease 1 (TREX1), or ERI1 (enhanced RNAi, also known as 3'hExo), wherein the exonuclease enzyme targets and trims the non-coding RNA.
PCT/US2023/072930 2022-08-25 2023-08-25 Methods of using exonucleases to make tiny rnas on argonaute proteins Ceased WO2024044759A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263400886P 2022-08-25 2022-08-25
US63/400,886 2022-08-25

Publications (2)

Publication Number Publication Date
WO2024044759A2 WO2024044759A2 (en) 2024-02-29
WO2024044759A3 true WO2024044759A3 (en) 2024-04-04

Family

ID=90014117

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/072930 Ceased WO2024044759A2 (en) 2022-08-25 2023-08-25 Methods of using exonucleases to make tiny rnas on argonaute proteins

Country Status (1)

Country Link
WO (1) WO2024044759A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024206999A2 (en) * 2023-03-30 2024-10-03 Ohio State Innovation Foundation Methods and compositions regarding using tinyrnas as biomarkers

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110201515A1 (en) * 2010-02-11 2011-08-18 Webster Philippa J Compositions and methods for the detection of small rnas
WO2022006276A1 (en) * 2020-06-30 2022-01-06 Ohio State Innovation Foundation Methods and compositions related to catalytic activation of human argonaute-3

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110201515A1 (en) * 2010-02-11 2011-08-18 Webster Philippa J Compositions and methods for the detection of small rnas
WO2022006276A1 (en) * 2020-06-30 2022-01-06 Ohio State Innovation Foundation Methods and compositions related to catalytic activation of human argonaute-3

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANTHONY K. HENRAS, CÉLIA PLISSON-CHASTANG, MARIE-FRANÇOISE O’DONOHUE, ANIRBAN CHAKRABORTY, PIERRE-EMMANUEL GLEIZES: "processing in eukaryotes", WILEY INTERDISCIPLINARY REVIEWS: RNA, JOHN WILEY & SONS LTD, UNITED KINGDOM, vol. 6, no. 2, 1 March 2015 (2015-03-01), United Kingdom , pages 225 - 242, XP093158388, ISSN: 1757-7004, DOI: 10.1002/wrna.1269 *
ELAD ELKAYAM; CLAUS-D. KUHN; ANTE TOCILJ; ASTRIDD. HAASE; EMILYM. GREENE; GREGORYJ. HANNON; LEEMOR JOSHUA-TOR;: "The Structure of Human Argonaute-2 in Complex with miR-20a", CELL, ELSEVIER, AMSTERDAM NL, vol. 150, no. 1, 17 May 2012 (2012-05-17), Amsterdam NL , pages 100 - 110, XP028400987, ISSN: 0092-8674, DOI: 10.1016/j.cell.2012.05.017 *
GEUNYOUNG SIM: "Manganese-dependent microRNA trimming by 3 5 exonucleases generates 14-nucleotidepsilon or shorter tiny RNAs", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, XP093158397, ISSN: 0027-8424, DOI: 10.1073/pnas.2214335119 *
WEI XIE: "Structure-function analysis of microRNA 3′-end trimming by Nibbler", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 117, no. 48, 1 December 2020 (2020-12-01), pages 30370 - 30379, XP093158391, ISSN: 0027-8424, DOI: 10.1073/pnas.2018156117 *

Also Published As

Publication number Publication date
WO2024044759A2 (en) 2024-02-29

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