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WO2024040211A3 - Bioproduction de flavonoïdes à l'aide d'hôtes de micro-organismes - Google Patents

Bioproduction de flavonoïdes à l'aide d'hôtes de micro-organismes Download PDF

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Publication number
WO2024040211A3
WO2024040211A3 PCT/US2023/072449 US2023072449W WO2024040211A3 WO 2024040211 A3 WO2024040211 A3 WO 2024040211A3 US 2023072449 W US2023072449 W US 2023072449W WO 2024040211 A3 WO2024040211 A3 WO 2024040211A3
Authority
WO
WIPO (PCT)
Prior art keywords
flavonoid
production
bioproduction
anthocyanins
flavonoids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2023/072449
Other languages
English (en)
Other versions
WO2024040211A2 (fr
Inventor
Nicholas BRIDEAU
Jingyi Li
Joshua BRITTON
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Debut Biotechnology Inc
Original Assignee
Debut Biotechnology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Debut Biotechnology Inc filed Critical Debut Biotechnology Inc
Priority to JP2025508901A priority Critical patent/JP2025527536A/ja
Priority to EP23855703.7A priority patent/EP4573202A2/fr
Priority to CA3265299A priority patent/CA3265299A1/fr
Priority to CN202380071946.4A priority patent/CN120019153A/zh
Publication of WO2024040211A2 publication Critical patent/WO2024040211A2/fr
Publication of WO2024040211A3 publication Critical patent/WO2024040211A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11009—Flavanone 3-dioxygenase (1.14.11.9), i.e. naringenin-3-dioxygenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11023—Flavonol synthase (1.14.11.23)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés impliqués dans la production de flavonoïdes, d'anthocyanines et d'autres composés organiques. L'invention concerne des cellules modifiées pour la production de flavonoïdes, d'anthocyanines et d'autres composés organiques, les cellules modifiées comprenant une ou plusieurs modifications génétiques qui augmentent la production de flavonoïdes en augmentant le flux métabolique pour les précurseurs de flavonoïdes et/ou en réduisant les pertes de carbone résultant de la production de sous-produits.
PCT/US2023/072449 2022-08-18 2023-08-18 Bioproduction de flavonoïdes à l'aide d'hôtes de micro-organismes Ceased WO2024040211A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2025508901A JP2025527536A (ja) 2022-08-18 2023-08-18 微生物宿主を使用するフラボノイドのバイオプロダクション
EP23855703.7A EP4573202A2 (fr) 2022-08-18 2023-08-18 Bioproduction de flavonoïdes à l'aide d'hôtes de micro-organismes
CA3265299A CA3265299A1 (fr) 2022-08-18 2023-08-18 Bioproduction de flavonoïdes à l'aide d'hôtes de micro-organismes
CN202380071946.4A CN120019153A (zh) 2022-08-18 2023-08-18 使用微生物宿主的类黄酮的生物生产

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263399005P 2022-08-18 2022-08-18
US63/399,005 2022-08-18

Publications (2)

Publication Number Publication Date
WO2024040211A2 WO2024040211A2 (fr) 2024-02-22
WO2024040211A3 true WO2024040211A3 (fr) 2024-05-23

Family

ID=89942304

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/072449 Ceased WO2024040211A2 (fr) 2022-08-18 2023-08-18 Bioproduction de flavonoïdes à l'aide d'hôtes de micro-organismes

Country Status (5)

Country Link
EP (1) EP4573202A2 (fr)
JP (1) JP2025527536A (fr)
CN (1) CN120019153A (fr)
CA (1) CA3265299A1 (fr)
WO (1) WO2024040211A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120310757B (zh) * 2024-11-15 2025-11-21 贵州师范大学 一种黄酮醇合酶、编码基因、表达载体、宿主菌及应用
CN119732320B (zh) * 2025-03-04 2025-06-06 云南林业职业技术学院 大花飞燕草组培快繁方法及应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007044992A2 (fr) * 2005-10-13 2007-04-19 Cornell University Polynucleotides codant des phenylpropanoides et des enzymes dans le cafeier, et processus de biosynthese de flavonoides
CN102304533A (zh) * 2011-08-22 2012-01-04 云南省农业科学院花卉研究所 黄烷酮3’,5’-羟化酶基因及其所编码的蛋白质
WO2020141230A1 (fr) * 2019-01-06 2020-07-09 Barrit Sarl Cellules hôtes recombinantes à production améliorée de dérivés de tetraketide

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007044992A2 (fr) * 2005-10-13 2007-04-19 Cornell University Polynucleotides codant des phenylpropanoides et des enzymes dans le cafeier, et processus de biosynthese de flavonoides
CN102304533A (zh) * 2011-08-22 2012-01-04 云南省农业科学院花卉研究所 黄烷酮3’,5’-羟化酶基因及其所编码的蛋白质
WO2020141230A1 (fr) * 2019-01-06 2020-07-09 Barrit Sarl Cellules hôtes recombinantes à production améliorée de dérivés de tetraketide

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE PROTEIN 27 March 2024 (2024-03-27), ANONYMOUS: "flavonol synthase/flavanone 3-hydroxylase [Daucus carota subsp. sativus]", XP093174890, Database accession no. XP017251246.1 *
LIU WEIXIN, FENG YI; YU SUHANG; FAN ZHENGQI; LI XINLEI; LI JIYUAN; YIN HENGFU: "The Flavonoid Biosynthesis Network in Plants", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL (MDPI), BASEL, CH, vol. 22, no. 23, Basel, CH , pages 12824, XP093174891, ISSN: 1422-0067, DOI: 10.3390/ijms222312824 *
MASUKAWA TAKANORI, CHEON KYEONG-SEONG; MIZUTA DAIKI; NAKATSUKA AKIRA; KOBAYASHI NOBUO: "Insertion of a Retrotransposon into a <i>Flavonoid 3'-hydroxylase</i> Homolog Confers the Red Root Character in the Radish (<i>Raphanus sativus</i> L. var. <i>longipinnatus</i> L. H. Bailey)", THE HORTICULTURE JOURNAL, vol. 87, no. 1, 1 January 2018 (2018-01-01), pages 89 - 96, XP093174893, ISSN: 2189-0102, DOI: 10.2503/hortj.OKD-075 *

Also Published As

Publication number Publication date
JP2025527536A (ja) 2025-08-22
CN120019153A (zh) 2025-05-16
WO2024040211A2 (fr) 2024-02-22
EP4573202A2 (fr) 2025-06-25
CA3265299A1 (fr) 2024-02-22

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