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WO2023210944A1 - Lateral flow assay strip for detecting drugs using drug detection reagent, and manufacturing method therefor - Google Patents

Lateral flow assay strip for detecting drugs using drug detection reagent, and manufacturing method therefor Download PDF

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Publication number
WO2023210944A1
WO2023210944A1 PCT/KR2023/002792 KR2023002792W WO2023210944A1 WO 2023210944 A1 WO2023210944 A1 WO 2023210944A1 KR 2023002792 W KR2023002792 W KR 2023002792W WO 2023210944 A1 WO2023210944 A1 WO 2023210944A1
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Prior art keywords
reagent
detection
pad
drug detection
lateral flow
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Ceased
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PCT/KR2023/002792
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French (fr)
Korean (ko)
Inventor
이병환
민홍기
김다미
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Philmedi Co ltd
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Philmedi Co ltd
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/8483—Investigating reagent band
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips

Definitions

  • the present invention relates to a lateral flow analysis strip for detecting narcotics using a drug detection reagent and a method for manufacturing the same.
  • a drug detection reagent refers to a reagent that can detect drugs through color change using a chemical method.
  • a single drug detection reagent can detect from one to several types of drugs, and the color changes for each drug, allowing you to check what type of drug is contained in the sample.
  • the purpose of the present invention is to solve the above problems, by embedding the drug detection reagent into the detection pad of the lateral flow analysis strip, portability is increased, and a lateral flow analysis strip for drug detection has convenience of use and convenience of detection, and The purpose is to provide its manufacturing method.
  • a sample pad 100 including a first porous membrane 110 and into which a sample is input; a detection pad 200 that receives a sample from the sample pad 100 and includes a second porous membrane 210 and a drug detection reagent 220 whose color changes by a chemical reaction; and an absorption pad 300 that absorbs the sample from the detection pad 200 and includes a third porous membrane 310.
  • a lateral flow analysis strip 10 for detecting narcotics is provided.
  • sample pad 100, the detection pad 200, and the absorption pad 300 may be positioned linearly and in contact with each other in that order.
  • the narcotic detection strip 10 further includes a substrate 400, and the sample pad 100, the detection pad 200, and the absorption pad 300 are each formed on the substrate 400. It may be.
  • the first porous membrane 110 includes one or more selected from the group consisting of glass fiber, cellulose, filter paper, woven mesh, polyethersulfone (PES), and chromatography paper, and 2
  • the porous membrane 210 includes one or more selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper
  • the third porous membrane 310 is It may contain one or more types selected from the group consisting of cellulose, super absorbent polymer (SAP), and cotton pad.
  • the drug detection reagent 220 includes Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent , Van Urk reagent, Vanillin reagent, Hofmann's reagent, and Valium reagent.
  • Dragendorff reagent Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van Urk reagent and Vanillin reagent.
  • the solid acid or non-volatile acid may be a weak acid.
  • Hofmann's reagent and Valium reagent may each contain a solid base or a non-volatile base.
  • the solid base or non-volatile base may be a weak base.
  • the drug detection reagent is Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent. It may include one or more types selected from the group consisting of.
  • the Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent are each the first It includes an organic solvent and water, and the first organic solvent may include one or more selected from the group consisting of acetone, ethanol, benzene, chloroform, dimethyl sulfoxide (DMSO), dimethyl-formamide (DMF), and toluene. there is.
  • the Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent are each second It contains at least one selected from the group consisting of organic solvents or water, and the second organic solvent is ethylene glycol monobutyl ether, ethylene glycol, isopropyl alcohol, and glycerol ( It may include one or more selected from the group consisting of glycerol).
  • the drug detection reagent includes at least one selected from the group consisting of Iodoplatinate reagent, Scott reagent, and Marquis, and at least one selected from the group consisting of Iodoplatinate reagent, Scott reagent, and Marquis reacts with the drug to produce a precipitate. And, the precipitate may remain in the second porous membrane 210.
  • a method for manufacturing a lateral flow analysis strip for detecting narcotics is provided, which includes a.
  • the drug detection reagent 220 includes Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent , a first drug detection reagent (221) comprising at least one selected from the group consisting of Van Urk reagent and Vanillin reagent; A second drug detection reagent (
  • the step of manufacturing the detection pad 200 in step (b) is (1) 1 selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper.
  • PES polyethersulfone
  • chromatography paper providing a second porous membrane (210) comprising more than one species; (2) preparing a mixture by mixing the first drug detection reagent 221 and a solid acid or non-volatile acid; and (3) manufacturing a detection pad 200 containing the first drug detection reagent 221 by applying the mixture on the second porous membrane 210 and drying it.
  • the step of manufacturing the detection pad 200 in step (b) is (1') selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper.
  • PES polyethersulfone
  • chromatography paper providing a second porous membrane (210) comprising one or more species; (2') preparing a mixture by mixing the second drug detection reagent 222 with a solid base or non-volatile base; and (3') manufacturing a detection pad 200 containing a second drug detection reagent 222 by applying the mixture on the second porous membrane 210 and drying it.
  • the step of manufacturing the detection pad 200 in step (b) is (1") selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper.
  • the step of manufacturing the detection pad 200 in step (b) is (i) 1 selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper.
  • PES polyethersulfone
  • chromatography paper providing a second porous membrane (210) comprising more than one species; (ii) preparing a solution containing the fourth drug detection reagent (224) from the fourth drug detection reagent precursor diluted with water; and (iii) manufacturing a detection pad 200 containing the fourth drug detection reagent 224 by applying the solution on the second porous membrane 210 and drying it.
  • the fourth drug detection reagent precursor may be diluted 2 to 5 times with water.
  • the lateral flow analysis strip for detecting narcotics of the present invention has the effect of being conveniently portable because detection is possible with a strip made of paper instead of carrying reagent chemicals and substrates that must be carried for detection.
  • FIG. 1 is a side schematic diagram of a drug detection strip according to an embodiment of the present invention.
  • Figure 2 is an actual image of a drug detection strip manufactured according to one embodiment of the present invention.
  • Figure 3 is a schematic diagram showing the process of manufacturing a drug detection strip according to one embodiment of the present invention.
  • Figure 4 shows the drug detection results of the Dragendorff's reagent strip prepared according to Example 1.
  • Figure 5 shows the drug detection results of the Ninhydrin reagent strip prepared according to Example 2.
  • Figure 6 shows the drug detection results of the Iodoplatinate reagent strip prepared according to Example 3.
  • first, second, etc. which will be used below, may be used to describe various components, but the components are not limited by the terms. The above terms are used only for the purpose of distinguishing one component from another.
  • a first component may be named a second component, and similarly, the second component may also be named a first component without departing from the scope of the present invention.
  • a component when referred to as being “on another component,” “formed on another component,” “located on another component,” or “stacked on another component,” It may be formed by being directly attached to the front or one side of the surface of the component, positioned, or stacked, but it should be understood that other components may further exist in the middle.
  • Figure 1 is a side schematic diagram of a drug detection strip according to an embodiment of the present invention
  • Figure 2 is an actual image of a drug detection strip manufactured according to an embodiment of the present invention.
  • the present invention includes a first porous membrane 110, a sample pad 100 for inserting a sample; a detection pad 200 that receives a sample from the sample pad 100 and includes a second porous membrane 210 and a drug detection reagent 220 whose color changes by a chemical reaction; and an absorption pad 300 that absorbs the sample from the detection pad 200 and includes a third porous membrane 310.
  • a lateral flow analysis strip 10 for detecting narcotics is provided.
  • sample pad 100, the detection pad 200, and the absorption pad 300 may be positioned linearly and in contact with each other in that order.
  • the narcotic detection strip 10 further includes a substrate 400, and the sample pad 100, the detection pad 200, and the absorption pad 300 are each formed on the substrate 400. It may be.
  • the first porous membrane 110 may include one or more selected from the group consisting of glass fiber, cellulose, filter paper, woven mesh, polyethersulfone (PES), and chromatography paper, Preferably, it may include glass fiber.
  • the second porous membrane 210 may include one or more selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper, and preferably May contain nitrocellulose.
  • the third porous membrane 310 may include one or more types selected from the group consisting of cellulose, superabsorbent polymer (SAP), and cotton pad, and may preferably include cellulose.
  • the drug detection reagent 220 includes Dragendorff's reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent , Van Urk reagent, Vanillin reagent, Hofmann's reagent, and Valium reagent, and may preferably include Dragendorff's reagent.
  • Dragendorff reagent Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van Urk reagent and Vanillin reagent.
  • the solid acid or non-volatile acid may be a weak acid.
  • the solid acid or non-volatile acid is maleic acid, formic acid, butyric acid, succinic acid, pyruvic acid, sorbic acid, and citric acid. acid), oxalic acid, DL-Tartaric acid, DL-Mandelic acid, and polystyrene sulfonate (sulfonated polystyrene). and may preferably include maleic acid.
  • Hofmann's reagent and Valium reagent may each contain a solid base or a non-volatile base.
  • the solid base or non-volatile base may be a weak base.
  • the solid base or non-volatile base is selected from the group consisting of potassium hydroxide, sodium hydroxide, ammonium hydroxide, nitrous acid, and hydrogen cyanide. It may include more than one species.
  • the drug detection reagent is Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent. It may include one or more types selected from the group consisting of.
  • the Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent are each the first It may include an organic solvent and water, and the first organic solvent includes one or more selected from the group consisting of acetone, ethanol, benzene, chloroform, dimethyl sulfoxide (DMSO), dimethyl-formamide (DMF), and toluene. can do.
  • the Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent are each second It may include one or more selected from the group consisting of organic solvents and water, and the second organic solvent is ethylene glycol monobutyl ether, ethylene glycol, isopropyl alcohol, and It may include one or more selected from the group consisting of glycerol.
  • the drug detection reagent includes at least one selected from the group consisting of Iodoplatinate reagent, Scott reagent, and Marquis, and at least one selected from the group consisting of Iodoplatinate reagent, Scott reagent, and Marquis reacts with the drug to produce a precipitate. And, the precipitate may remain in the second porous membrane 210.
  • Figure 3 is a schematic diagram showing the process of manufacturing a drug detection strip according to one embodiment of the present invention.
  • the present invention includes the steps of (a) providing a substrate 400; (b) manufacturing the sample pad 100, detection pad 200, and absorption pad 300, respectively; and (c) forming the sample pad 100, the detection pad 200, and the absorption pad 300 on the substrate 400 in order to contact each other, so that the sample pad 100 and the detection pad ( 200) and manufacturing a drug analysis strip 10 in which the absorption pad 300 is positioned linearly, wherein the detection pad 200 is a drug detection reagent 220 whose color changes by a chemical reaction. It provides a method of manufacturing a lateral flow analysis strip for detecting narcotics, which includes a.
  • the drug detection reagent 220 includes Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent , a first drug detection reagent (221) comprising at least one selected from the group consisting of Van Urk reagent and Vanillin reagent; A second drug detection reagent (
  • the step of manufacturing the detection pad 200 in step (b) is (1) 1 selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper.
  • PES polyethersulfone
  • chromatography paper providing a second porous membrane (210) comprising more than one species; (2) preparing a mixture by mixing the first drug detection reagent 221 and a solid acid or non-volatile acid; and (3) manufacturing a detection pad 200 containing the first drug detection reagent 221 by applying the mixture on the second porous membrane 210 and drying it.
  • the step of manufacturing the detection pad 200 in step (b) is (1') selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper.
  • PES polyethersulfone
  • chromatography paper providing a second porous membrane (210) comprising one or more species; (2') preparing a mixture by mixing the second drug detection reagent 222 with a solid base or non-volatile base; and (3') manufacturing a detection pad 200 containing a second drug detection reagent 222 by applying the mixture on the second porous membrane 210 and drying it.
  • the step of manufacturing the detection pad 200 in step (b) is (1") selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper.
  • the step of manufacturing the detection pad 200 in step (b) is (i) 1 selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper.
  • PES polyethersulfone
  • chromatography paper providing a second porous membrane (210) comprising more than one species; (ii) preparing a solution containing the fourth drug detection reagent (224) from the fourth drug detection reagent precursor diluted with water; and (iii) manufacturing a detection pad 200 containing the fourth drug detection reagent 224 by applying the solution on the second porous membrane 210 and drying it.
  • the fourth drug detection reagent precursor may be diluted 2 to 5 times with water. If the precursor is diluted more than 2 times, it is undesirable because the manufactured fourth drug detection reagent has already formed a precipitate regardless of the reaction with the drug, making it impossible to confirm whether or not the drug has been detected. If the precursor is diluted more than five times, the prepared fourth drug detection reagent is undesirable. It is not desirable because the fourth drug detection reagent forms a small amount of precipitate after reacting with drugs, making it difficult to confirm whether drugs have been detected.
  • Figure 1 is a side schematic diagram of a drug detection strip according to an embodiment of the present invention
  • Figure 2 is an actual image of a drug detection strip manufactured according to an embodiment of the present invention
  • Figure 3 is a schematic diagram showing the process of manufacturing a drug detection strip according to one embodiment of the present invention. Referring to Figures 1 to 3, a drug detection strip using a drug detection reagent used with one or more types selected from the group consisting of strong acids and strong bases was manufactured.
  • a backing card from BORE DA BIOTECH was used as the substrate 400, and the backing card has a three-layer structure of polyvinyl chloride (PVC)/polymer adhesive/release paper.
  • the backing card was prepared by cutting it into 8 mm in width and 60 mm in height.
  • Glass fiber (BORE DA BIOTECH, 8964) was used as the first porous membrane 110 of the sample pad 100, cut to a size of 8 mm wide and 20 mm long.
  • Nitrocellulose membrane (VIV9025100R, PALL company) was used as the second porous membrane 210 of the detection pad 200 by cutting it to a size of 8 mm horizontally and 14 mm vertically.
  • Maleic acid solution was prepared by adding 1.0 g of maleic acid to 2 mL of distilled water and stirring. Add 0.06 g of Bismuth subnitrate to 1 ml of the Maleic acid solution and stir to prepare a Maleic acid + Bismuth subnitrate solution, and add 0.12 g of Potassium iodide to 1 ml of the Maleic acid solution and stir to prepare a Maleic acid + Potassium iodide solution. did. Drahendorff's solution was prepared by mixing the Maleic acid + Bismuth subnitrate solution and the Maleic acid + Potassium iodide solution in a 1:1 volume ratio and stirring until an orange color appeared.
  • a detection pad 200 containing a drug detection reagent 220 was manufactured by applying 2.5 ⁇ m of the Drahendorff's solution onto the second porous membrane 210 and drying it.
  • Cellulose membrane (BORE DA BIOTECH, Grade 222) was used as the third porous membrane 310 of the absorbent pad 300, cut to a size of 8 mm in width and 30 mm in height.
  • the release paper of the backing card was peeled off, and the detection pad 200 was placed on the adhesive surface of the backing card so that the drug detection reagent of the detection pad was facing upward. Afterwards, the sample pad 100 and the absorption pad 300 were each overlapped with the detection pad 200 by 2 mm and placed on the adhesive surface of the backing card to prepare a Dragendorff's reagent strip.
  • a solution was prepared by mixing 0.7 mL of ethyl alcohol and 0.3 mL of distilled water.
  • Ninhydrin solution was prepared by adding 100 mg of Ninhydrin to 1 ml of the above solution and stirring.
  • Ninhydrin reagent strips were manufactured in the same manner as in Example 1, except that the detection pad 200 containing the drug detection reagent 220 was manufactured using the Ninhydrin solution instead of using Drahendorff's solution.
  • Hydrogen hexachloroplatinate solution was prepared by adding 40 mg of Hydrogen hexachloroplatinate (IV) to 1 ml of distilled water and stirring.
  • Potassium iodide solution was prepared by adding 133.33 mg of Potassium iodide to 1 ml of distilled water and stirring.
  • Iodoplatinate solution was prepared by mixing the Hydrogen hexachloroplatinate solution and the Potassium iodide solution in a 1:1 volume ratio and stirring.
  • Iodoplatinate strips were prepared in the same manner as in Example 1, except that the detection pad 200 containing the drug detection reagent 220 was manufactured by applying the Iodoplatinate solution through a line dispenser instead of applying Drahendorff's solution at 2.5 ⁇ m. Manufactured.
  • Test Example 1 Checking whether drugs are detected in Dragendorff's reagent strips
  • Figure 4 shows the drug detection results of the Dragendorff's reagent strip prepared according to Example 1.
  • the Dragendorff's reagent strip before the drug detection experiment the Dragendorff's reagent strip after adding the reagent not containing the detection substance (negative)
  • the Dragendorff's reagent strip after adding the reagent containing the detection substance (0.5% methamphetamine (MA)).
  • MA methamphetamine
  • the Dragendorff's reagent strip prepared according to Example 1 clearly shows a color difference between when a sample not containing a detection substance is used (negative) and when a sample containing a detection substance is used (positive). You can see what appears.
  • the strong acid/strong base is volatile and evaporates when embedded in the strip and does not participate in detection, or the strong acid/strong base damages the paper (i.e., catches fire, etc.) There was a problem with not embedding it in the strip (which generates smoke).
  • the Dragendorff's reagent strip of the present invention can be manufactured by using maleic acid, a solid acid or non-volatile acid, instead of hydrochloric acid, a strong acid, to manufacture a strip with built-in Dragendorff's reagent, and the Dragendorff's reagent manufactured using the maleic acid is It can be confirmed that narcotics are detected well.
  • Test Example 2 Confirmation of drug detection in Ninhydrin reagent strip
  • Figure 5 shows the drug detection results of the Ninhydrin reagent strip prepared according to Example 2.
  • the Ninhydrin reagent strip before conducting the drug detection experiment the Ninhydrin reagent strip after adding a reagent not containing the detection substance (negative)
  • the Ninhydrin reagent strip prepared according to Example 2 clearly shows a color difference between when a sample not containing a detection substance is used (negative) and when a sample containing a detection substance is used (positive). You can see what appears.
  • samples using high concentrations of organic solvents such as acetone and aldehyde, such as Ninhydrin reagent had a problem in that the paper was damaged by the organic solvent and could not be embedded in the strip.
  • the Ninhydrin reagent strip of the present invention can produce a strip with Ninhydrin reagent embedded in it by reducing the amount of organic solvent using water, and the Ninhydrin reagent strip manufactured using a solution containing water and organic solvent is narcotic. It can be confirmed that it is detected well.
  • Test Example 3 Confirmation of drug detection in Iodoplatinate reagent strip
  • Figure 6 shows the drug detection results of the Iodoplatinate reagent strip prepared according to Example 3.
  • Iodoplatinate reagent strips before conducting a drug detection experiment Iodoplatinate reagent strips after adding a reagent not containing the detection substance (negative), and detection substances with different concentrations (0.03%, 0.05% methamphetamine (MA)).
  • the Iodoplatinate reagent strip prepared according to Example 3 clearly shows a color difference between when a sample not containing a detection substance is used (negative) and when a sample containing a detection substance is used (positive). You can see what appears.
  • Iodoplatinate reagent causes precipitation when the sample contains a detection substance (drug). If the precipitated solution does not remain on the second porous membrane 210 of the detection pad 200, the detection result cannot be confirmed.
  • the Iodoplatinate reagent strip of the present invention can manufacture a strip with an embedded Iodoplatinate reagent by manufacturing the Iodoplatinate reagent from a precursor of the Iodoplatinate reagent diluted using distilled water, and the solution precipitated after reaction with the detection material is deposited on the detection pad (200 ) can be confirmed to remain in the second porous membrane 210.

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  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

Disclosed are a lateral flow assay strip for detecting drugs using a drug detection reagent, and a manufacturing method therefor. The lateral flow assay strip (10) for detecting drugs comprises: a sample pad (100) comprising a first porous film (110) and having a sample inputted therein; a detection pad (200) receiving the sample from the sample pad (100) and comprising a second porous film (210) and a drug detection reagent (220) which changes color as a result of a chemical reaction; and an absorption pad (300) absorbing the sample from the detection pad (200) and comprising a third porous film (310). Thus, the present invention has the effect of increasing the portability and convenience of use of the drug detection reagent.

Description

마약탐지 시약을 이용한 마약류 검출용 측방유동 분석 스트립 및 그의 제조방법Lateral flow analysis strip for drug detection using drug detection reagent and method for manufacturing the same

본 발명은 마약탐지 시약을 이용한 마약류 검출용 측방유동 분석 스트립 및 그의 제조방법에 관한 것이다.The present invention relates to a lateral flow analysis strip for detecting narcotics using a drug detection reagent and a method for manufacturing the same.

마약탐지 시약은 화학적 방법을 이용하여 색 변화를 통해 마약류를 검출할 수 있는 시약을 의미한다. 하나의 마약탐지 시약이 검출할 수 있는 마약류는 1종부터 여러 종을 검출할 수 있으며, 마약마다 변화하는 색이 달라 시료에 포함된 마약이 어떤 종류인지 확인할 수 있다.A drug detection reagent refers to a reagent that can detect drugs through color change using a chemical method. A single drug detection reagent can detect from one to several types of drugs, and the color changes for each drug, allowing you to check what type of drug is contained in the sample.

그러나, 기존 마약탐지 시약을 사용하기 위해서는 플라스틱, 유리 또는 광석으로 제작된 흰색의 반구 틀이 필요하며, 상기 마약탐지 시약을 구성하는 화학물질을 모두 들고 다녀야 하는 문제점이 있다.However, in order to use existing drug detection reagents, a white hemispherical frame made of plastic, glass, or ore is required, and there is a problem in that all chemicals constituting the drug detection reagents must be carried.

따라서, 마약탐지 시약을 간편하게 휴대할 수 있으며 상기 마약탐지 시약을 간편하게 사용할 수 있는 방법에 관한 연구가 필요하다.Therefore, research is needed on how to easily carry and use drug detection reagents.

본 발명의 목적은 상기 문제점들을 해결하기 위한 것으로, 마약탐지 시약을 측방유동 분석 스트립의 검출 패드에 내장함으로써 휴대성이 증가하고, 사용의 편의성 및 검출의 편의성을 갖는 마약류 검출용 측방유동 분석 스트립 및 그의 제조방법을 제공하는데 있다.The purpose of the present invention is to solve the above problems, by embedding the drug detection reagent into the detection pad of the lateral flow analysis strip, portability is increased, and a lateral flow analysis strip for drug detection has convenience of use and convenience of detection, and The purpose is to provide its manufacturing method.

본 발명의 일 측면에 따르면, 제1 다공성 막(110)을 포함하고, 시료가 투입되기 위한 시료 패드(100); 상기 시료 패드(100)로부터 시료를 공급받고, 제2 다공성 막(210) 및 화학적 반응에 의해 색이 변화되는 마약탐지 시약(220)을 포함하는 검출 패드(200); 및 상기 검출 패드(200)로부터 시료를 흡수하고, 제3 다공성 막(310)을 포함하는 흡수 패드(300);를 포함하는 마약류 검출용 측방유동 분석 스트립(10)이 제공된다.According to one aspect of the present invention, a sample pad 100 including a first porous membrane 110 and into which a sample is input; a detection pad 200 that receives a sample from the sample pad 100 and includes a second porous membrane 210 and a drug detection reagent 220 whose color changes by a chemical reaction; and an absorption pad 300 that absorbs the sample from the detection pad 200 and includes a third porous membrane 310. A lateral flow analysis strip 10 for detecting narcotics is provided.

또한, 상기 시료 패드(100), 상기 검출 패드(200) 및 상기 흡수 패드(300)가 순서대로 서로 접하여 선형으로 위치하는 것일 수 있다.Additionally, the sample pad 100, the detection pad 200, and the absorption pad 300 may be positioned linearly and in contact with each other in that order.

또한, 상기 마약류 검출 스트립(10)이 기판(400)을 추가로 포함하고, 상기 샘플 패드(100), 상기 검출 패드(200) 및 상기 흡수 패드(300)가 각각 상기 기판(400) 상에 형성된 것일 수 있다.In addition, the narcotic detection strip 10 further includes a substrate 400, and the sample pad 100, the detection pad 200, and the absorption pad 300 are each formed on the substrate 400. It may be.

또한, 상기 제1 다공성 막(110)이 글래스 파이버, 셀룰로오스, 필터 페이퍼, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하고, 상기 제2 다공성 막(210)이 니트로셀룰로오스, 셀룰로오스, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하고, 상기 제3 다공성 막(310)이 셀룰로오스, 고흡수성수지(SAP) 및 Cotton pad으로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있다.In addition, the first porous membrane 110 includes one or more selected from the group consisting of glass fiber, cellulose, filter paper, woven mesh, polyethersulfone (PES), and chromatography paper, and 2 The porous membrane 210 includes one or more selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper, and the third porous membrane 310 is It may contain one or more types selected from the group consisting of cellulose, super absorbent polymer (SAP), and cotton pad.

또한, 상기 마약탐지 시약(220)이 Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van Urk reagent, Vanillin reagent, Hofmann's reagent 및 Valium reagent로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있다.In addition, the drug detection reagent 220 includes Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent , Van Urk reagent, Vanillin reagent, Hofmann's reagent, and Valium reagent.

또한, 상기 Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van Urk reagent 및 Vanillin reagent가 각각 고체산 또는 비휘발성 산을 포함할 수 있다.In addition, the Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van Urk reagent and Vanillin reagent. may each include a solid acid or a non-volatile acid.

또한, 상기 고체산 또는 비휘발성 산이 약산(weak acid)일 수 있다.Additionally, the solid acid or non-volatile acid may be a weak acid.

또한, 상기 Hofmann's reagent 및 Valium reagent가 각각 고체염기 또는 비휘발성 염기를 포함할 수 있다.Additionally, the Hofmann's reagent and Valium reagent may each contain a solid base or a non-volatile base.

또한, 상기 고체염기 또는 비휘발성 염기가 약염기(weak base)일 수 있다.Additionally, the solid base or non-volatile base may be a weak base.

또한, 상기 마약탐지 시약이 Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent 및 Zwikker reagent로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있다.In addition, the drug detection reagent is Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent. It may include one or more types selected from the group consisting of.

또한, 상기 Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent 및 Zwikker reagent가 각각 제1 유기용매 및 물을 포함하고, 상기 제1 유기용매는 아세톤, 에탄올, 벤젠, 클로로포름, 디메틸 설폭사이드(DMSO), 디메틸-포름아미드(DMF) 및 톨루엔으로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있다.In addition, the Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent are each the first It includes an organic solvent and water, and the first organic solvent may include one or more selected from the group consisting of acetone, ethanol, benzene, chloroform, dimethyl sulfoxide (DMSO), dimethyl-formamide (DMF), and toluene. there is.

또한, 상기 Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent 및 Zwikker reagent가 각각 제2 유기용매 또는 물로 이루어진 군으로부터 선택된 1종 이상을 포함하고, 상기 제2 유기용매는 에틸렌 글리콜 모노부틸 에더(ethylene glycol monobutyl ether), 에틸렌 글리콜(ethylene glycol), 이소프로필알코올(isopropyl alcohol) 및 글리세롤(glycerol)로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있다.In addition, the Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent are each second It contains at least one selected from the group consisting of organic solvents or water, and the second organic solvent is ethylene glycol monobutyl ether, ethylene glycol, isopropyl alcohol, and glycerol ( It may include one or more selected from the group consisting of glycerol).

또한, 상기 마약탐지 시약이 Iodoplatinate reagent, Scott reagent 및 Marquis로 이루어진 군으로부터 선택된 1종 이상을 포함하고, 상기 Iodoplatinate reagent, Scott reagent 및 Marquis로 이루어진 군으로부터 선택된 1종 이상이 마약과 반응하여 침전물이 생성되고, 상기 침전물이 상기 제2 다공성 막(210)에 남는 것일 수 있다.In addition, the drug detection reagent includes at least one selected from the group consisting of Iodoplatinate reagent, Scott reagent, and Marquis, and at least one selected from the group consisting of Iodoplatinate reagent, Scott reagent, and Marquis reacts with the drug to produce a precipitate. And, the precipitate may remain in the second porous membrane 210.

본 발명의 다른 하나의 측면에 따르면, (a) 기판(400)을 제공하는 단계; (b) 샘플 패드(100), 검출 패드(200) 및 흡수 패드(300)를 각각 제조하는 단계; 및 (c) 상기 기판(400) 상에 상기 샘플 패드(100), 상기 검출 패드(200) 및 상기 흡수 패드(300)를 서로 접하도록 순서대로 형성하여 상기 샘플 패드(100), 상기 검출 패드(200) 및 상기 흡수 패드(300)가 선형으로 위치하는 마약류 분석 스트립(10)을 제조하는 단계;를 포함하고, 상기 검출 패드(200)는 화학적 반응에 의해 색이 변화되는 마약탐지 시약(220)을 포함하는 것인, 마약류 검출용 측방유동 분석 스트립의 제조방법이 제공된다.According to another aspect of the present invention, (a) providing a substrate 400; (b) manufacturing the sample pad 100, detection pad 200, and absorption pad 300, respectively; and (c) forming the sample pad 100, the detection pad 200, and the absorption pad 300 on the substrate 400 in order to contact each other, so that the sample pad 100 and the detection pad ( 200) and manufacturing a drug analysis strip 10 in which the absorption pad 300 is positioned linearly, wherein the detection pad 200 is a drug detection reagent 220 whose color changes by a chemical reaction. A method for manufacturing a lateral flow analysis strip for detecting narcotics is provided, which includes a.

또한, 상기 마약탐지 시약(220)이 Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van Urk reagent 및 Vanillin reagent로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제1 마약탐지 시약(221); Hofmann's reagent 및 Valium reagent로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제2 마약탐지 시약(222); Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent 및 Zwikker reagent로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제3 마약탐지 시약(223); 및 Iodoplatinate reagent, Scott reagent, 및 Marquis로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제4 마약탐지 시약(224);으로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있다.In addition, the drug detection reagent 220 includes Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent , a first drug detection reagent (221) comprising at least one selected from the group consisting of Van Urk reagent and Vanillin reagent; A second drug detection reagent (222) comprising at least one selected from the group consisting of Hofmann's reagent and Valium reagent; One selected from the group consisting of Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent A third drug detection reagent (223) including the above; and a fourth drug detection reagent 224 including one or more selected from the group consisting of Iodoplatinate reagent, Scott reagent, and Marquis.

또한, 상기 단계 (b)의 상기 검출 패드(200)를 제조하는 단계가 (1) 니트로셀룰로오스, 셀룰로오스, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제2 다공성 막(210)을 제공하는 단계; (2) 상기 제1 마약탐지 시약(221)과 고체산 또는 비휘발성 산을 혼합하여 혼합물을 제조하는 단계; 및 (3) 상기 제2 다공성 막(210) 상에 상기 혼합물을 도포 후 건조시켜 제1 마약탐지 시약(221)을 포함하는 검출 패드(200)를 제조하는 단계;를 포함할 수 있다.In addition, the step of manufacturing the detection pad 200 in step (b) is (1) 1 selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper. providing a second porous membrane (210) comprising more than one species; (2) preparing a mixture by mixing the first drug detection reagent 221 and a solid acid or non-volatile acid; and (3) manufacturing a detection pad 200 containing the first drug detection reagent 221 by applying the mixture on the second porous membrane 210 and drying it.

또한, 상기 단계 (b)의 상기 검출 패드(200)를 제조하는 단계가 (1') 니트로셀룰로오스, 셀룰로오스, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제2 다공성 막(210)을 제공하는 단계; (2') 상기 제2 마약탐지 시약(222)과 고체염기 또는 비휘발성 염기를 혼합하여 혼합물을 제조하는 단계; 및 (3') 상기 제2 다공성 막(210) 상에 상기 혼합물을 도포 후 건조시켜 제2 마약탐지 시약(222)을 포함하는 검출 패드(200)를 제조하는 단계;를 포함할 수 있다.In addition, the step of manufacturing the detection pad 200 in step (b) is (1') selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper. providing a second porous membrane (210) comprising one or more species; (2') preparing a mixture by mixing the second drug detection reagent 222 with a solid base or non-volatile base; and (3') manufacturing a detection pad 200 containing a second drug detection reagent 222 by applying the mixture on the second porous membrane 210 and drying it.

또한, 상기 단계 (b)의 상기 검출 패드(200)를 제조하는 단계가 (1") 니트로셀룰로오스, 셀룰로오스, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제2 다공성 막(210)을 제공하는 단계; (2") 유기용매 및 물을 포함하는 용액에 상기 제3 마약탐지 시약(223)을 혼합하여 혼합물을 제조하는 단계; 및 (3") 상기 제2 다공성 막(210) 상에 상기 혼합물을 도포 후 건조시켜 제3 마약탐지 시약(223)을 포함하는 검출 패드(200)를 제조하는 단계;를 포함할 수 있다.In addition, the step of manufacturing the detection pad 200 in step (b) is (1") selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper. Providing a second porous membrane 210 containing one or more species; (2") preparing a mixture by mixing the third drug detection reagent 223 with a solution containing an organic solvent and water; and (3") manufacturing a detection pad 200 containing a third drug detection reagent 223 by applying the mixture on the second porous membrane 210 and drying it.

또한, 상기 단계 (b)의 상기 검출 패드(200)를 제조하는 단계가 (i) 니트로셀룰로오스, 셀룰로오스, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제2 다공성 막(210)을 제공하는 단계; (ii) 물에 의해 희석된 제4 마약탐지 시약 전구체로부터 제4 마약탐지 시약(224)을 포함하는 용액을 제조하는 단계; 및 (iii) 상기 제2 다공성 막(210) 상에 상기 용액을 도포 후 건조시켜 제4 마약탐지 시약(224)을 포함하는 검출 패드(200)를 제조하는 단계;를 포함할 수 있다.In addition, the step of manufacturing the detection pad 200 in step (b) is (i) 1 selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper. providing a second porous membrane (210) comprising more than one species; (ii) preparing a solution containing the fourth drug detection reagent (224) from the fourth drug detection reagent precursor diluted with water; and (iii) manufacturing a detection pad 200 containing the fourth drug detection reagent 224 by applying the solution on the second porous membrane 210 and drying it.

또한, 상기 제4 마약탐지 시약 전구체가 물에 의해 2 내지 5배 희석된 것일 수 있다.Additionally, the fourth drug detection reagent precursor may be diluted 2 to 5 times with water.

본 발명의 마약류 검출용 측방유동 분석 스트립은 검출을 위해 들고 다녀야 하는 Reagent 구성 화학물질 및 기판을 들고 다니지 않고 종이로 구성된 스트립이 있으면 검출이 가능하여 휴대성이 편리한 효과가 있다.The lateral flow analysis strip for detecting narcotics of the present invention has the effect of being conveniently portable because detection is possible with a strip made of paper instead of carrying reagent chemicals and substrates that must be carried for detection.

이 도면들은 본 발명의 예시적인 실시예를 설명하는데 참조하기 위함이므로, 본 발명의 기술적 사상을 첨부한 도면에 한정해서 해석하여서는 아니 된다.Since these drawings are for reference in explaining exemplary embodiments of the present invention, the technical idea of the present invention should not be interpreted as limited to the attached drawings.

도 1은 본원발명 하나의 실시예에 따른 마약류 검출 스트립의 측면 모식도이다.1 is a side schematic diagram of a drug detection strip according to an embodiment of the present invention.

도 2는 본원발명 하나의 실시예에 따라 제조된 마약류 검출 스트립의 실제 이미지이다.Figure 2 is an actual image of a drug detection strip manufactured according to one embodiment of the present invention.

도 3은 본원발명 하나의 실시예에 따른 마약류 검출 스트립을 제조하는 과정을 나타낸 모식도이다.Figure 3 is a schematic diagram showing the process of manufacturing a drug detection strip according to one embodiment of the present invention.

도 4는 실시예 1에 따라 제조된 Dragendorff's reagent 스트립의 마약검출 결과를 나타낸 것이다.Figure 4 shows the drug detection results of the Dragendorff's reagent strip prepared according to Example 1.

도 5는 실시예 2에 따라 제조된 Ninhydrin reagent 스트립의 마약검출 결과를 나타낸 것이다.Figure 5 shows the drug detection results of the Ninhydrin reagent strip prepared according to Example 2.

도 6은 실시예 3에 따라 제조된 Iodoplatinate reagent 스트립의 마약검출 결과를 나타낸 것이다.Figure 6 shows the drug detection results of the Iodoplatinate reagent strip prepared according to Example 3.

본 발명은 다양한 변환을 가할 수 있고 여러 가지 실시예를 가질 수 있는 바, 특정 실시예들을 예시하고 상세한 설명에 상세하게 설명하고자 한다. 그러나, 이는 본 발명을 특정한 실시 형태에 대해 한정하려는 것이 아니며, 본 발명의 사상 및 기술 범위에 포함되는 모든 변환, 균등물 내지 대체물을 포함하는 것으로 이해되어야 한다. 본 발명을 설명함에 있어서 관련된 공지 기술에 대한 구체적인 설명이 본 발명의 요지를 흐릴 수 있다고 판단되는 경우 그 상세한 설명을 생략한다.Since the present invention can be modified in various ways and can have various embodiments, specific embodiments will be illustrated and described in detail in the detailed description. However, this is not intended to limit the present invention to specific embodiments, and should be understood to include all transformations, equivalents, and substitutes included in the spirit and technical scope of the present invention. In describing the present invention, if it is determined that a detailed description of related known technologies may obscure the gist of the present invention, the detailed description will be omitted.

또한, 이하에서 사용될 제1, 제2 등과 같이 서수를 포함하는 용어는 다양한 구성요소들을 설명하는데 사용될 수 있지만, 상기 구성요소들은 상기 용어들에 의해 한정되지는 않는다. 상기 용어들은 하나의 구성요소를 다른 구성요소로부터 구별하는 목적으로만 사용된다. 예를 들어, 본 발명의 권리 범위를 벗어나지 않으면서 제1 구성요소는 제2 구성요소로 명명될 수 있고, 유사하게 제2 구성요소도 제1 구성요소로 명명될 수 있다. Additionally, terms including ordinal numbers, such as first, second, etc., which will be used below, may be used to describe various components, but the components are not limited by the terms. The above terms are used only for the purpose of distinguishing one component from another. For example, a first component may be named a second component, and similarly, the second component may also be named a first component without departing from the scope of the present invention.

또한, 어떤 구성요소가 “다른 구성요소 상에", "다른 구성요소 상에 형성되어", "다른 구성요소 상에 위치하여"또는 "다른 구성요소 상에 적층되어" 있다고 언급된 때에는, 그 다른 구성요소의 표면 상의 전면 또는 일면에 직접 부착되어 형성되어, 위치하여 있거나 또는 적층되어 있을 수도 있지만, 중간에 다른 구성요소가 더 존재할 수도 있다고 이해되어야 할 것이다.Additionally, when a component is referred to as being “on another component,” “formed on another component,” “located on another component,” or “stacked on another component,” It may be formed by being directly attached to the front or one side of the surface of the component, positioned, or stacked, but it should be understood that other components may further exist in the middle.

단수의 표현은 문맥상 명백하게 다르게 뜻하지 않는 한, 복수의 표현을 포함한다. 본 출원에서, "포함하다" 또는 "가지다" 등의 용어는 명세서상에 기재된 특징, 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것이 존재함을 지정하려는 것이지, 하나 또는 그 이상의 다른 특징들이나 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것들의 존재 또는 부가 가능성을 미리 배제하지 않는 것으로 이해되어야 한다.Singular expressions include plural expressions unless the context clearly dictates otherwise. In this application, terms such as “comprise” or “have” are intended to designate the presence of features, numbers, steps, operations, components, parts, or combinations thereof described in the specification, but are not intended to indicate the presence of one or more other features. It should be understood that this does not exclude in advance the possibility of the existence or addition of elements, numbers, steps, operations, components, parts, or combinations thereof.

이하, 본 발명의 마약탐지 시약을 이용한 마약류 검출용 측방유동 분석 스트립 및 그의 제조방법에 대하여 상세히 설명하기로 한다. 다만, 이는 예시로서 제시되는 것으로, 이에 의해 본 발명이 제한되지는 않으며 본 발명은 후술할 청구범위의 범주에 의해 정의될 뿐이다.Hereinafter, the lateral flow analysis strip for drug detection using the drug detection reagent of the present invention and its manufacturing method will be described in detail. However, this is presented as an example, and the present invention is not limited thereby, and the present invention is only defined by the scope of the claims to be described later.

도 1은 본원발명 하나의 실시예에 따른 마약류 검출 스트립의 측면 모식도이고, 도 2는 본원발명 하나의 실시예에 따라 제조된 마약류 검출 스트립의 실제 이미지이다. Figure 1 is a side schematic diagram of a drug detection strip according to an embodiment of the present invention, and Figure 2 is an actual image of a drug detection strip manufactured according to an embodiment of the present invention.

도 1 및 2를 참고하면, 본 발명은 제1 다공성 막(110)을 포함하고, 시료가 투입되기 위한 시료 패드(100); 상기 시료 패드(100)로부터 시료를 공급받고, 제2 다공성 막(210) 및 화학적 반응에 의해 색이 변화되는 마약탐지 시약(220)을 포함하는 검출 패드(200); 및 상기 검출 패드(200)로부터 시료를 흡수하고, 제3 다공성 막(310)을 포함하는 흡수 패드(300);를 포함하는 마약류 검출용 측방유동 분석 스트립(10)을 제공한다.Referring to Figures 1 and 2, the present invention includes a first porous membrane 110, a sample pad 100 for inserting a sample; a detection pad 200 that receives a sample from the sample pad 100 and includes a second porous membrane 210 and a drug detection reagent 220 whose color changes by a chemical reaction; and an absorption pad 300 that absorbs the sample from the detection pad 200 and includes a third porous membrane 310. A lateral flow analysis strip 10 for detecting narcotics is provided.

또한, 상기 시료 패드(100), 상기 검출 패드(200) 및 상기 흡수 패드(300)가 순서대로 서로 접하여 선형으로 위치하는 것일 수 있다.Additionally, the sample pad 100, the detection pad 200, and the absorption pad 300 may be positioned linearly and in contact with each other in that order.

또한, 상기 마약류 검출 스트립(10)이 기판(400)을 추가로 포함하고, 상기 샘플 패드(100), 상기 검출 패드(200) 및 상기 흡수 패드(300)가 각각 상기 기판(400) 상에 형성된 것일 수 있다.In addition, the narcotic detection strip 10 further includes a substrate 400, and the sample pad 100, the detection pad 200, and the absorption pad 300 are each formed on the substrate 400. It may be.

또한, 상기 제1 다공성 막(110)이 글래스 파이버, 셀룰로오스, 필터 페이퍼, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있고, 바람직하게는 글래스 파이버를 포함할 수 있다.In addition, the first porous membrane 110 may include one or more selected from the group consisting of glass fiber, cellulose, filter paper, woven mesh, polyethersulfone (PES), and chromatography paper, Preferably, it may include glass fiber.

또한, 상기 제2 다공성 막(210)이 니트로셀룰로오스, 셀룰로오스, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있고, 바람직하게는 니트로셀룰로오스를 포함할 수 있다.In addition, the second porous membrane 210 may include one or more selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper, and preferably May contain nitrocellulose.

또한, 상기 제3 다공성 막(310)이 셀룰로오스, 고흡수성수지(SAP) 및 Cotton pad로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있고, 바람직하게는 셀룰로오스를 포함할 수 있다.Additionally, the third porous membrane 310 may include one or more types selected from the group consisting of cellulose, superabsorbent polymer (SAP), and cotton pad, and may preferably include cellulose.

또한, 상기 마약탐지 시약(220)이 Dragendorff's reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van Urk reagent, Vanillin reagent, Hofmann's reagent 및 Valium reagent로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있고, 바람직하게는 Dragendorff's reagent를 포함할 수 있다.In addition, the drug detection reagent 220 includes Dragendorff's reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent , Van Urk reagent, Vanillin reagent, Hofmann's reagent, and Valium reagent, and may preferably include Dragendorff's reagent.

또한, 상기 Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van Urk reagent 및 Vanillin reagent가 각각 고체산 또는 비휘발성 산을 포함할 수 있다.In addition, the Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van Urk reagent and Vanillin reagent. may each include a solid acid or a non-volatile acid.

또한, 상기 고체산 또는 비휘발성 산이 약산(weak acid)일 수 있다.Additionally, the solid acid or non-volatile acid may be a weak acid.

또한, 상기 고체산 또는 비휘발성 산이 말레산(maleic acid), 포름산(formic acid), 부티르산(butyric acid), 석신산(succinic acid), 피루브산(pyruvic acid), 소르빈산(sorbic acid), 시트르산(citric acid), 옥살산(oxalic acid), DL-타타르산(DL-Tartaric acid), DL-만델산(DL-Mandelic acid) 및 폴리스티렌 설포네이트(sulfonated polystyrene)로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있고, 바람직하게는 말레산(maleic acid)을 포함할 수 있다.In addition, the solid acid or non-volatile acid is maleic acid, formic acid, butyric acid, succinic acid, pyruvic acid, sorbic acid, and citric acid. acid), oxalic acid, DL-Tartaric acid, DL-Mandelic acid, and polystyrene sulfonate (sulfonated polystyrene). and may preferably include maleic acid.

또한, 상기 Hofmann's reagent 및 Valium reagent가 각각 고체염기 또는 비휘발성 염기를 포함할 수 있다.Additionally, the Hofmann's reagent and Valium reagent may each contain a solid base or a non-volatile base.

또한, 상기 고체염기 또는 비휘발성 염기가 약염기(weak base)일 수 있다.Additionally, the solid base or non-volatile base may be a weak base.

또한, 상기 고체염기 또는 비휘발성 염기가 수산화 칼륨(potassium hydroxide), 수산화 나트륨(sodium hydroxide), 수산화 암모늄(ammonium hydroxide), 아질산(nitrous acid) 및 사이안화 수소(hydrogen cyanide)로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있다.In addition, the solid base or non-volatile base is selected from the group consisting of potassium hydroxide, sodium hydroxide, ammonium hydroxide, nitrous acid, and hydrogen cyanide. It may include more than one species.

또한, 상기 마약탐지 시약이 Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent 및 Zwikker reagent로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있다.In addition, the drug detection reagent is Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent. It may include one or more types selected from the group consisting of.

또한, 상기 Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent 및 Zwikker reagent가 각각 제1 유기용매 및 물을 포함할 수 있고, 상기 제1 유기용매가 아세톤, 에탄올, 벤젠, 클로로포름, 디메틸 설폭사이드(DMSO), 디메틸-포름아미드(DMF) 및 톨루엔으로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있다.In addition, the Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent are each the first It may include an organic solvent and water, and the first organic solvent includes one or more selected from the group consisting of acetone, ethanol, benzene, chloroform, dimethyl sulfoxide (DMSO), dimethyl-formamide (DMF), and toluene. can do.

또한, 상기 Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent 및 Zwikker reagent가 각각 제2 유기용매 및 물로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있고, 상기 제2 유기용매가 에틸렌 글리콜 모노부틸 에더(ethylene glycol monobutyl ether), 에틸렌 글리콜(ethylene glycol), 이소프로필알코올(isopropyl alcohol) 및 글리세롤(glycerol)로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있다.In addition, the Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent are each second It may include one or more selected from the group consisting of organic solvents and water, and the second organic solvent is ethylene glycol monobutyl ether, ethylene glycol, isopropyl alcohol, and It may include one or more selected from the group consisting of glycerol.

또한, 상기 마약탐지 시약이 Iodoplatinate reagent, Scott reagent 및 Marquis로 이루어진 군으로부터 선택된 1종 이상을 포함하고, 상기 Iodoplatinate reagent, Scott reagent 및 Marquis로 이루어진 군으로부터 선택된 1종 이상이 마약과 반응하여 침전물이 생성되고, 상기 침전물이 상기 제2 다공성 막(210)에 남는 것일 수 있다.In addition, the drug detection reagent includes at least one selected from the group consisting of Iodoplatinate reagent, Scott reagent, and Marquis, and at least one selected from the group consisting of Iodoplatinate reagent, Scott reagent, and Marquis reacts with the drug to produce a precipitate. And, the precipitate may remain in the second porous membrane 210.

도 3은 본원발명 하나의 실시예에 따른 마약류 검출 스트립을 제조하는 과정을 나타낸 모식도이다.Figure 3 is a schematic diagram showing the process of manufacturing a drug detection strip according to one embodiment of the present invention.

도 3을 참고하면, 본 발명은 (a) 기판(400)을 제공하는 단계; (b) 샘플 패드(100), 검출 패드(200) 및 흡수 패드(300)를 각각 제조하는 단계; 및 (c) 상기 기판(400) 상에 상기 샘플 패드(100), 상기 검출 패드(200) 및 상기 흡수 패드(300)를 서로 접하도록 순서대로 형성하여 상기 샘플 패드(100), 상기 검출 패드(200) 및 상기 흡수 패드(300)가 선형으로 위치하는 마약류 분석 스트립(10)을 제조하는 단계;를 포함하고, 상기 검출 패드(200)는 화학적 반응에 의해 색이 변화되는 마약탐지 시약(220)을 포함하는 것인, 마약류 검출용 측방유동 분석 스트립의 제조방법을 제공한다.Referring to Figure 3, the present invention includes the steps of (a) providing a substrate 400; (b) manufacturing the sample pad 100, detection pad 200, and absorption pad 300, respectively; and (c) forming the sample pad 100, the detection pad 200, and the absorption pad 300 on the substrate 400 in order to contact each other, so that the sample pad 100 and the detection pad ( 200) and manufacturing a drug analysis strip 10 in which the absorption pad 300 is positioned linearly, wherein the detection pad 200 is a drug detection reagent 220 whose color changes by a chemical reaction. It provides a method of manufacturing a lateral flow analysis strip for detecting narcotics, which includes a.

또한, 상기 마약탐지 시약(220)이 Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van Urk reagent 및 Vanillin reagent로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제1 마약탐지 시약(221); Hofmann's reagent 및 Valium reagent로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제2 마약탐지 시약(222); Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent 및 Zwikker reagent로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제3 마약탐지 시약(223); 및 Iodoplatinate reagent, Scott reagent 및 Marquis로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제4 마약탐지 시약(224);으로 이루어진 군으로부터 선택된 1종 이상을 포함할 수 있다.In addition, the drug detection reagent 220 includes Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent , a first drug detection reagent (221) comprising at least one selected from the group consisting of Van Urk reagent and Vanillin reagent; A second drug detection reagent (222) comprising at least one selected from the group consisting of Hofmann's reagent and Valium reagent; One selected from the group consisting of Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent A third drug detection reagent (223) including the above; and a fourth drug detection reagent 224 including one or more selected from the group consisting of Iodoplatinate reagent, Scott reagent, and Marquis.

또한, 상기 단계 (b)의 상기 검출 패드(200)를 제조하는 단계가 (1) 니트로셀룰로오스, 셀룰로오스, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제2 다공성 막(210)을 제공하는 단계; (2) 상기 제1 마약탐지 시약(221)과 고체산 또는 비휘발성 산을 혼합하여 혼합물을 제조하는 단계; 및 (3) 상기 제2 다공성 막(210) 상에 상기 혼합물을 도포 후 건조시켜 제1 마약탐지 시약(221)을 포함하는 검출 패드(200)를 제조하는 단계;를 포함할 수 있다.In addition, the step of manufacturing the detection pad 200 in step (b) is (1) 1 selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper. providing a second porous membrane (210) comprising more than one species; (2) preparing a mixture by mixing the first drug detection reagent 221 and a solid acid or non-volatile acid; and (3) manufacturing a detection pad 200 containing the first drug detection reagent 221 by applying the mixture on the second porous membrane 210 and drying it.

또한, 상기 단계 (b)의 상기 검출 패드(200)를 제조하는 단계가 (1') 니트로셀룰로오스, 셀룰로오스, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제2 다공성 막(210)을 제공하는 단계; (2') 상기 제2 마약탐지 시약(222)과 고체염기 또는 비휘발성 염기를 혼합하여 혼합물을 제조하는 단계; 및 (3') 상기 제2 다공성 막(210) 상에 상기 혼합물을 도포 후 건조시켜 제2 마약탐지 시약(222)을 포함하는 검출 패드(200)를 제조하는 단계;를 포함할 수 있다.In addition, the step of manufacturing the detection pad 200 in step (b) is (1') selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper. providing a second porous membrane (210) comprising one or more species; (2') preparing a mixture by mixing the second drug detection reagent 222 with a solid base or non-volatile base; and (3') manufacturing a detection pad 200 containing a second drug detection reagent 222 by applying the mixture on the second porous membrane 210 and drying it.

또한, 상기 단계 (b)의 상기 검출 패드(200)를 제조하는 단계가 (1") 니트로셀룰로오스, 셀룰로오스, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제2 다공성 막(210)을 제공하는 단계; (2") 유기용매 및 물을 포함하는 용액에 상기 제3 마약탐지 시약(223)을 혼합하여 혼합물을 제조하는 단계; 및 (3") 상기 제2 다공성 막(210) 상에 상기 혼합물을 도포 후 건조시켜 제3 마약탐지 시약(223)을 포함하는 검출 패드(200)를 제조하는 단계;를 포함할 수 있다.In addition, the step of manufacturing the detection pad 200 in step (b) is (1") selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper. Providing a second porous membrane 210 containing one or more species; (2") preparing a mixture by mixing the third drug detection reagent 223 with a solution containing an organic solvent and water; and (3") manufacturing a detection pad 200 containing a third drug detection reagent 223 by applying the mixture on the second porous membrane 210 and drying it.

또한, 상기 단계 (b)의 상기 검출 패드(200)를 제조하는 단계가 (i) 니트로셀룰로오스, 셀룰로오스, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제2 다공성 막(210)을 제공하는 단계; (ii) 물에 의해 희석된 제4 마약탐지 시약 전구체로부터 제4 마약탐지 시약(224)을 포함하는 용액을 제조하는 단계; 및 (iii) 상기 제2 다공성 막(210) 상에 상기 용액을 도포 후 건조시켜 제4 마약탐지 시약(224)을 포함하는 검출 패드(200)를 제조하는 단계;를 포함할 수 있다.In addition, the step of manufacturing the detection pad 200 in step (b) is (i) 1 selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper. providing a second porous membrane (210) comprising more than one species; (ii) preparing a solution containing the fourth drug detection reagent (224) from the fourth drug detection reagent precursor diluted with water; and (iii) manufacturing a detection pad 200 containing the fourth drug detection reagent 224 by applying the solution on the second porous membrane 210 and drying it.

또한, 상기 제4 마약탐지 시약 전구체가 물에 의해 2 내지 5배 희석된 것일 수 있다. 상기 전구체가 2배 이하로 희석될 경우 제조된 제4 마약탐지 시약이 마약류와 반응과 무관하게 이미 침전이 형성되어 마약류 검출 여부를 확인할 수 없어 바람직하지 않고, 5배를 초과하여 희석될 경우 제조된 제4 마약탐지 시약이 마약류와 반응 후 침전물을 적게 형성하여 마약류 검출 여부를 확인하기 어려우므로 바람직하지 않다. Additionally, the fourth drug detection reagent precursor may be diluted 2 to 5 times with water. If the precursor is diluted more than 2 times, it is undesirable because the manufactured fourth drug detection reagent has already formed a precipitate regardless of the reaction with the drug, making it impossible to confirm whether or not the drug has been detected. If the precursor is diluted more than five times, the prepared fourth drug detection reagent is undesirable. It is not desirable because the fourth drug detection reagent forms a small amount of precipitate after reacting with drugs, making it difficult to confirm whether drugs have been detected.

[실시예] [Example]

이하, 본 발명을 실시예를 들어 더욱 상세하게 설명하도록 한다. 그러나 이는 예시를 위한 것으로서 이에 의하여 본 발명의 범위가 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail through examples. However, this is for illustrative purposes only and does not limit the scope of the present invention.

실시예 1: Dragendorff's reagent 스트립 제조Example 1: Preparation of Dragendorff's reagent strips

도 1은 본원발명 하나의 실시예에 따른 마약류 검출 스트립의 측면 모식도이고, 도 2는 본원발명 하나의 실시예에 따라 제조된 마약류 검출 스트립의 실제 이미지이다. 도 3은 본원발명 하나의 실시예에 따른 마약류 검출 스트립을 제조하는 과정을 나타낸 모식도이다. 도 1 내지 3을 참고하여 강산 및 강염기로 이루어진 군으로부터 선택된 1종 이상과 함께 사용하는 마약탐지 시약 사용하는 마약류 검출 스트립을 제조하였다.Figure 1 is a side schematic diagram of a drug detection strip according to an embodiment of the present invention, and Figure 2 is an actual image of a drug detection strip manufactured according to an embodiment of the present invention. Figure 3 is a schematic diagram showing the process of manufacturing a drug detection strip according to one embodiment of the present invention. Referring to Figures 1 to 3, a drug detection strip using a drug detection reagent used with one or more types selected from the group consisting of strong acids and strong bases was manufactured.

BORE DA BIOTECH 사의 Backing card를 기판(400)으로 사용하였으며 상기 backing card는 폴리비닐클로라이드(PVC)/고분자 점착제/이형지의 3층 구조로 구성된 것이다. 상기 Backing card를 가로 8 mm, 세로 60 mm로 잘라 준비하였다.A backing card from BORE DA BIOTECH was used as the substrate 400, and the backing card has a three-layer structure of polyvinyl chloride (PVC)/polymer adhesive/release paper. The backing card was prepared by cutting it into 8 mm in width and 60 mm in height.

샘플 패드(100)의 제1 다공성 막(110)으로 글래스 파이버(BORE DA BIOTECH 사, 8964)를 가로 8 mm, 세로 20 mm 크기로 재단하여 사용하였다.Glass fiber (BORE DA BIOTECH, 8964) was used as the first porous membrane 110 of the sample pad 100, cut to a size of 8 mm wide and 20 mm long.

검출 패드(200)의 제2 다공성 막(210)으로 Nitrocellulose membrane(PALL 사, VIV9025100R)를 가로 8mm, 세로 14mm 크기로 재단하여 사용하였다.Nitrocellulose membrane (VIV9025100R, PALL company) was used as the second porous membrane 210 of the detection pad 200 by cutting it to a size of 8 mm horizontally and 14 mm vertically.

증류수 2 mL에 Maleic acid를 1.0 g 첨가 후 교반하여 Maleic acid 용액을 제조하였다. 상기 Maleic acid 용액 1 ml에 Bismuth subnitrate를 0.06 g 첨가 후 교반하여 Maleic acid + Bismuth subnitrate 용액을 제조하고, 상기 Maleic acid 용액 1 ml에 Potassium iodide를 0.12 g 첨가 후 교반하여 Maleic acid + Potassium iodide 용액을 제조하였다. 상기 Maleic acid + Bismuth subnitrate 용액과 상기 Maleic acid + Potassium iodide 용액을 1:1 부피비율로 혼합 후 주황색이 나올 때까지 교반하여 Drahendorff’s 용액을 제조하였다.Maleic acid solution was prepared by adding 1.0 g of maleic acid to 2 mL of distilled water and stirring. Add 0.06 g of Bismuth subnitrate to 1 ml of the Maleic acid solution and stir to prepare a Maleic acid + Bismuth subnitrate solution, and add 0.12 g of Potassium iodide to 1 ml of the Maleic acid solution and stir to prepare a Maleic acid + Potassium iodide solution. did. Drahendorff's solution was prepared by mixing the Maleic acid + Bismuth subnitrate solution and the Maleic acid + Potassium iodide solution in a 1:1 volume ratio and stirring until an orange color appeared.

상기 제2 다공성 막(210) 상에 상기 Drahendorff’s 용액을 2.5 μm 인가 후 건조하여 마약탐지 시약(220)을 포함하는 검출 패드(200)를 제조하였다.A detection pad 200 containing a drug detection reagent 220 was manufactured by applying 2.5 μm of the Drahendorff's solution onto the second porous membrane 210 and drying it.

흡수 패드(300)의 제3 다공성 막(310)으로 Cellulose membrane(BORE DA BIOTECH 사, Grade 222)을 가로 8 mm, 세로 30 mm 크기로 재단하여 사용하였다.Cellulose membrane (BORE DA BIOTECH, Grade 222) was used as the third porous membrane 310 of the absorbent pad 300, cut to a size of 8 mm in width and 30 mm in height.

상기 Backing card의 이형지를 박리하고, 상기 검출 패드(200)를 상기 검출 패드의 마약탐지 시약이 위로 향하도록 상기 Backing card 점착면 위에 위치시켰다. 이후, 샘플 패드(100) 및 흡수 패드(300)를 상기 검출 패드(200)와 각각 2 mm 겹치기(Overlapping)하여 상기 Backing card 점착면 위에 위치시켜 Dragendorff's reagent 스트립을 제조하였다.The release paper of the backing card was peeled off, and the detection pad 200 was placed on the adhesive surface of the backing card so that the drug detection reagent of the detection pad was facing upward. Afterwards, the sample pad 100 and the absorption pad 300 were each overlapped with the detection pad 200 by 2 mm and placed on the adhesive surface of the backing card to prepare a Dragendorff's reagent strip.

실시예 2: Ninhydrin reagent 스트립 제조Example 2: Preparation of Ninhydrin reagent strips

Ethyl alcohol 0.7 mL와 증류수 0.3 ml을 혼합하여 용액을 제조하였다. 1 ml의 상기 용액에 Ninhydrin을 100 mg 첨가 후 교반하여 Ninhydrin 용액을 제조하였다.A solution was prepared by mixing 0.7 mL of ethyl alcohol and 0.3 mL of distilled water. Ninhydrin solution was prepared by adding 100 mg of Ninhydrin to 1 ml of the above solution and stirring.

Drahendorff’s 용액을 사용한 것 대신에 상기 Ninhydrin 용액을 사용하여 마약탐지 시약(220)을 포함하는 검출 패드(200)를 제조한 것을 제외하고는 실시예 1과 동일한 방법으로 Ninhydrin reagent 스트립을 제조하였다.Ninhydrin reagent strips were manufactured in the same manner as in Example 1, except that the detection pad 200 containing the drug detection reagent 220 was manufactured using the Ninhydrin solution instead of using Drahendorff's solution.

실시예 3: Iodoplatinate reagent 스트립 제조Example 3: Preparation of Iodoplatinate reagent strips

증류수 1 ml에 Hydrogen hexachloroplatinate(IV)를 40 mg 첨가 후 교반하여 Hydrogen hexachloroplatinate 용액을 제조하고, 증류수 1 ml에 Potassium iodide 133.33 mg 첨가 후 교반하여 Potassium iodide 용액을 제조하였다. 상기 Hydrogen hexachloroplatinate 용액과 상기 Potassium iodide 용액을 1:1 부피비율로 혼합 후 교반하여 Iodoplatinate 용액을 제조하였다.Hydrogen hexachloroplatinate solution was prepared by adding 40 mg of Hydrogen hexachloroplatinate (IV) to 1 ml of distilled water and stirring. Potassium iodide solution was prepared by adding 133.33 mg of Potassium iodide to 1 ml of distilled water and stirring. Iodoplatinate solution was prepared by mixing the Hydrogen hexachloroplatinate solution and the Potassium iodide solution in a 1:1 volume ratio and stirring.

Drahendorff’s 용액을 2.5 μm 인가한 것 대신에 상기 Iodoplatinate 용액을 Line dispensor를 인가하여 마약탐지 시약(220)을 포함하는 검출 패드(200)를 제조한 것을 제외하고는 실시예 1과 동일한 방법으로 Iodoplatinate 스트립을 제조하였다.Iodoplatinate strips were prepared in the same manner as in Example 1, except that the detection pad 200 containing the drug detection reagent 220 was manufactured by applying the Iodoplatinate solution through a line dispenser instead of applying Drahendorff's solution at 2.5 μm. Manufactured.

[시험예][Test example]

시험예 1: Dragendorff's reagent 스트립의 마약검출 여부 확인Test Example 1: Checking whether drugs are detected in Dragendorff's reagent strips

도 4는 실시예 1에 따라 제조된 Dragendorff's reagent 스트립의 마약검출 결과를 나타낸 것이다. 상세하게는 마약검출 실험을 진행하기 전의 Dragendorff's reagent 스트립, 검출물질을 포함하지 않는 시약을 투입한 후의 Dragendorff's reagent 스트립(음성) 및 검출물질(0.5 %의 메스암페타민(MA))을 포함하는 시약을 투입한 후의 Dragendorff's reagent 스트립(양성)의 이미지를 나타낸 것이다.Figure 4 shows the drug detection results of the Dragendorff's reagent strip prepared according to Example 1. In detail, the Dragendorff's reagent strip before the drug detection experiment, the Dragendorff's reagent strip after adding the reagent not containing the detection substance (negative), and the Dragendorff's reagent strip after adding the reagent containing the detection substance (0.5% methamphetamine (MA)). This shows an image of the subsequent Dragendorff's reagent strip (positive).

도 4를 참고하면, 실시예 1에 따라 제조된 Dragendorff's reagent 스트립은 검출물질을 포함하지 않은 시료를 사용할 경우(음성)와, 검출물질을 포함하는 시료를 사용할 경우(양성)의 색 차이가 명확하게 나타나는 것을 확인할 수 있다.Referring to FIG. 4, the Dragendorff's reagent strip prepared according to Example 1 clearly shows a color difference between when a sample not containing a detection substance is used (negative) and when a sample containing a detection substance is used (positive). You can see what appears.

또한, Dragendorff's reagent와 같은 강산/강염기를 함께 사용하는 시료는 상기 강산/강염기가 휘발성이여서 스트립에 내장할 시 휘발되어 검출에 관여하지 못하거나, 상기 강산/강염기가 종이를 손상시켜(불이 붙거나 연기를 발생시키는) 스트립에 내장하지 못한 문제점이 있었다.In addition, for samples using a strong acid/strong base together, such as Dragendorff's reagent, the strong acid/strong base is volatile and evaporates when embedded in the strip and does not participate in detection, or the strong acid/strong base damages the paper (i.e., catches fire, etc.) There was a problem with not embedding it in the strip (which generates smoke).

그러나, 본 발명의 Dragendorff's reagent 스트립은 강산인 Hydrochloric acid 대신 고체산 또는 비휘발성 산인 Maleic acid를 사용하여 제작함으로써 Dragendorff's reagent가 내장된 스트립을 제조할 수 있으며, 상기 Maleic acid를 사용하여 제조된 Dragendorff's reagent가 마약류를 잘 검출하는 것을 확인할 수 있다.However, the Dragendorff's reagent strip of the present invention can be manufactured by using maleic acid, a solid acid or non-volatile acid, instead of hydrochloric acid, a strong acid, to manufacture a strip with built-in Dragendorff's reagent, and the Dragendorff's reagent manufactured using the maleic acid is It can be confirmed that narcotics are detected well.

시험예 2: Ninhydrin reagent 스트립의 마약검출 여부 확인Test Example 2: Confirmation of drug detection in Ninhydrin reagent strip

도 5는 실시예 2에 따라 제조된 Ninhydrin reagent 스트립의 마약검출 결과를 나타낸 것이다. 상세하게는 마약검출 실험을 진행하기 전의 Ninhydrin reagent 스트립, 검출물질을 포함하지 않는 시약을 투입한 후의 Ninhydrin reagent 스트립(음성) 및 검출물질(1 %의 암페타민)을 포함하는 시약을 투입한 후의 Ninhydrin reagent 스트립(양성)의 이미지를 나타낸 것이다.Figure 5 shows the drug detection results of the Ninhydrin reagent strip prepared according to Example 2. In detail, the Ninhydrin reagent strip before conducting the drug detection experiment, the Ninhydrin reagent strip after adding a reagent not containing the detection substance (negative), and the Ninhydrin reagent strip after adding a reagent containing the detection substance (1% amphetamine). This shows an image of a strip (positive).

도 4를 참고하면, 실시예 2에 따라 제조된 Ninhydrin reagent 스트립은 검출물질을 포함하지 않은 시료를 사용할 경우(음성)와, 검출물질을 포함하는 시료를 사용할 경우(양성)의 색 차이가 명확하게 나타나는 것을 확인할 수 있다.Referring to FIG. 4, the Ninhydrin reagent strip prepared according to Example 2 clearly shows a color difference between when a sample not containing a detection substance is used (negative) and when a sample containing a detection substance is used (positive). You can see what appears.

또한, Ninhydrin reagent와 같이 고 농도의 아세톤, 알데히드와 같은 유기용매를 함께 사용하는 시료는 상기 유기용매에 의해 종이가 손상되어 스트립에 내장할 수 없는 문제점이 있었다.In addition, samples using high concentrations of organic solvents such as acetone and aldehyde, such as Ninhydrin reagent, had a problem in that the paper was damaged by the organic solvent and could not be embedded in the strip.

그러나, 본 발명의 Ninhydrin reagent 스트립은 물을 사용하여 유기용매의 양을 줄임으로써 Ninhydrin reagent가 내장된 스트립을 제조할 수 있으며, 물 및 유기용매를 포함하는 용액을 사용하여 제조된 Ninhydrin reagent 스트립이 마약류를 잘 검출하는 것을 확인할 수 있다.However, the Ninhydrin reagent strip of the present invention can produce a strip with Ninhydrin reagent embedded in it by reducing the amount of organic solvent using water, and the Ninhydrin reagent strip manufactured using a solution containing water and organic solvent is narcotic. It can be confirmed that it is detected well.

시험예 3: Iodoplatinate reagent 스트립의 마약검출 여부 확인Test Example 3: Confirmation of drug detection in Iodoplatinate reagent strip

도 6은 실시예 3에 따라 제조된 Iodoplatinate reagent 스트립의 마약검출 결과를 나타낸 것이다. 상세하게는 마약검출 실험을 진행하기 전의 Iodoplatinate reagent 스트립, 검출물질을 포함하지 않는 시약을 투입한 후의 Iodoplatinate reagent 스트립(음성), 농도가 다른 검출물질(0.03 %, 0.05 %의 메스암페타민(MA))을 포함하는 시약을 각각 투입한 후의 Ninhydrin reagent 스트립(양성)의 이미지를 나타낸 것이다.Figure 6 shows the drug detection results of the Iodoplatinate reagent strip prepared according to Example 3. In detail, Iodoplatinate reagent strips before conducting a drug detection experiment, Iodoplatinate reagent strips after adding a reagent not containing the detection substance (negative), and detection substances with different concentrations (0.03%, 0.05% methamphetamine (MA)). This shows an image of a Ninhydrin reagent strip (positive) after each reagent containing it was added.

도 6을 참고하면, 실시예 3에 따라 제조된 Iodoplatinate reagent 스트립은 검출물질을 포함하지 않은 시료를 사용할 경우(음성)와, 검출물질을 포함하는 시료를 사용할 경우(양성)의 색 차이가 명확하게 나타나는 것을 확인할 수 있다.Referring to FIG. 6, the Iodoplatinate reagent strip prepared according to Example 3 clearly shows a color difference between when a sample not containing a detection substance is used (negative) and when a sample containing a detection substance is used (positive). You can see what appears.

또한, Iodoplatinate reagent는 시료에 검출물질(마약)을 포함할 경우, 침전이 발생하는 것이다. 침전된 용액이 검출 패드(200)의 제2 다공성 막(210)에 남지 않으면 검출 결과를 확인할 수 없다.Additionally, Iodoplatinate reagent causes precipitation when the sample contains a detection substance (drug). If the precipitated solution does not remain on the second porous membrane 210 of the detection pad 200, the detection result cannot be confirmed.

따라서, 본 발명의 Iodoplatinate reagent 스트립은 증류수를 이용해 희석된 Iodoplatinate reagent의 전구체로부터 Iodoplatinate reagent를 제조함으로써 Iodoplatinate reagent가 내장된 스트립을 제조할 수 있으며, 검출물질과의 반응 이후 침전된 용액이 검출 패드(200)의 제2 다공성 막(210)에 남는 것을 확인할 수 있다.Therefore, the Iodoplatinate reagent strip of the present invention can manufacture a strip with an embedded Iodoplatinate reagent by manufacturing the Iodoplatinate reagent from a precursor of the Iodoplatinate reagent diluted using distilled water, and the solution precipitated after reaction with the detection material is deposited on the detection pad (200 ) can be confirmed to remain in the second porous membrane 210.

본 발명의 범위는 상기 상세한 설명보다는 후술하는 특허청구범위에 의하여 나타내어지며, 특허청구범위의 의미 및 범위 그리고 그 균등 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.The scope of the present invention is indicated by the claims described below rather than the detailed description above, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts should be construed as being included in the scope of the present invention. do.

Claims (20)

제1 다공성 막(110)을 포함하고, 시료가 투입되기 위한 샘플 패드(100);A sample pad 100 including a first porous membrane 110 and into which a sample is introduced; 상기 샘플 패드(100)로부터 시료를 공급받고, 제2 다공성 막(210) 및 화학적 반응에 의해 색이 변화되는 마약탐지 시약(220)을 포함하는 검출 패드(200); 및a detection pad 200 that receives a sample from the sample pad 100 and includes a second porous membrane 210 and a drug detection reagent 220 whose color changes by a chemical reaction; and 상기 검출 패드(200)로부터 시료를 흡수하고, 제3 다공성 막(310)을 포함하는 흡수 패드(300);를an absorption pad 300 that absorbs the sample from the detection pad 200 and includes a third porous membrane 310; 포함하는 마약류 검출용 측방유동 분석 스트립(10).Lateral flow analysis strip (10) for detecting narcotics, including: 제1항에 있어서,According to paragraph 1, 상기 샘플 패드(100), 상기 검출 패드(200) 및 상기 흡수 패드(300)가 순서대로 서로 접하여 선형으로 위치하는 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립.A lateral flow analysis strip for detecting narcotics, characterized in that the sample pad 100, the detection pad 200, and the absorption pad 300 are positioned linearly in contact with each other in that order. 제2항에 있어서,According to paragraph 2, 상기 마약류 검출 스트립(10)이 기판(400)을 추가로 포함하고,The drug detection strip 10 further includes a substrate 400, 상기 샘플 패드(100), 상기 검출 패드(200) 및 상기 흡수 패드(300)가 각각 상기 기판(400) 상에 형성된 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립.A lateral flow analysis strip for detecting narcotics, wherein the sample pad 100, the detection pad 200, and the absorption pad 300 are each formed on the substrate 400. 제1항에 있어서,According to paragraph 1, 상기 제1 다공성 막(110)이 글래스 파이버, 셀룰로오스, 필터 페이퍼, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하고,The first porous membrane 110 includes one or more selected from the group consisting of glass fiber, cellulose, filter paper, woven mesh, polyethersulfone (PES), and chromatography paper, 상기 제2 다공성 막(210)이 니트로셀룰로오스, 셀룰로오스, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하고,The second porous membrane 210 includes one or more selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper, 상기 제3 다공성 막(310)이 셀룰로오스, 고흡수성수지(SAP) 및 Cotton pad로 이루어진 군으로부터 선택된 1종 이상을 포함하는 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립.A lateral flow analysis strip for detecting narcotics, wherein the third porous membrane 310 includes at least one selected from the group consisting of cellulose, superabsorbent polymer (SAP), and cotton pad. 제1항에 있어서,According to paragraph 1, 상기 마약탐지 시약(220)이 Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van Urk reagent, Vanillin reagent, Hofmann's reagent 및 Valium reagent로 이루어진 군으로부터 선택된 1종 이상을 포함하는 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립.The drug detection reagent 220 includes Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, and Libermann reagent. , Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van A lateral flow analysis strip for detecting narcotics, comprising at least one selected from the group consisting of Urk reagent, Vanillin reagent, Hofmann's reagent, and Valium reagent. 제5항에 있어서,According to clause 5, 상기 Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van Urk reagent 및 Vanillin reagent가 각각 고체산 또는 비휘발성 산을 포함하는 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립.The Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van Urk reagent and Vanillin reagent respectively. A lateral flow analysis strip for detecting narcotics, comprising a solid acid or a non-volatile acid. 제6항에 있어서,According to clause 6, 상기 고체산 또는 비휘발성 산이 약산(weak acid)인 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립.A lateral flow analysis strip for detecting narcotics, characterized in that the solid acid or non-volatile acid is a weak acid. 제5항에 있어서,According to clause 5, 상기 Hofmann's reagent 및 Valium reagent가 각각 고체염기 또는 비휘발성 염기를 포함하는 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립.A lateral flow analysis strip for detecting narcotics, wherein the Hofmann's reagent and Valium reagent each contain a solid base or a non-volatile base. 제8항에 있어서,According to clause 8, 상기 고체염기 또는 비휘발성 염기가 약염기(weak base)인 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립.A lateral flow analysis strip for detecting narcotics, characterized in that the solid base or non-volatile base is a weak base. 제1항에 있어서,According to paragraph 1, 상기 마약탐지 시약이 Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent 및 Zwikker reagent로 이루어진 군으로부터 선택된 1종 이상을 포함하는 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립.The drug detection reagent consists of Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent. A lateral flow analysis strip for detecting narcotics, comprising at least one selected from the group. 제10항에 있어서,According to clause 10, 상기 Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent 및 Zwikker reagent가 각각 제1 유기용매 및 물을 포함하고,The Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent are each used as the first organic solvent. and water, 상기 제1 유기용매는 아세톤, 에탄올, 벤젠, 클로로포름, 디메틸 설폭사이드(DMSO), 디메틸-포름아미드(DMF) 및 톨루엔으로 이루어진 군으로부터 선택된 1종 이상을 포함하는 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립.The first organic solvent is a lateral flow for detecting narcotics, characterized in that it contains at least one selected from the group consisting of acetone, ethanol, benzene, chloroform, dimethyl sulfoxide (DMSO), dimethyl-formamide (DMF), and toluene. Analysis strips. 제10항에 있어서,According to clause 10, 상기 Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent 및 Zwikker reagent가 각각 제2 유기용매 또는 물로 이루어진 군으로부터 선택된 1종 이상을 포함하고, The Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent are each used as a second organic solvent. or at least one selected from the group consisting of water, 상기 제2 유기용매는 에틸렌 글리콜 모노부틸 에더(ethylene glycol monobutyl ether), 에틸렌 글리콜(ethylene glycol), 이소프로필알코올(isopropyl alcohol) 및 글리세롤(glycerol)로 이루어진 군으로부터 선택된 1종 이상을 포함하는 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립.The second organic solvent is characterized in that it contains at least one selected from the group consisting of ethylene glycol monobutyl ether, ethylene glycol, isopropyl alcohol, and glycerol. Lateral flow analysis strip for drug detection. 제1항에 있어서,According to paragraph 1, 상기 마약탐지 시약이 Iodoplatinate reagent, Scott reagent 및 Marquis로 이루어진 군으로부터 선택된 1종 이상을 포함하고, The drug detection reagent includes at least one selected from the group consisting of Iodoplatinate reagent, Scott reagent, and Marquis, 상기 Iodoplatinate reagent, Scott reagent 및 Marquis로 이루어진 군으로부터 선택된 1종 이상이 마약과 반응하여 침전물이 생성되고,At least one selected from the group consisting of Iodoplatinate reagent, Scott reagent and Marquis reacts with the drug to produce a precipitate, 상기 침전물이 상기 제2 다공성 막(210)에 남는 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립.A lateral flow analysis strip for detecting narcotics, characterized in that the sediment remains on the second porous membrane (210). (a) 기판(400)을 제공하는 단계;(a) providing a substrate 400; (b) 샘플 패드(100), 검출 패드(200) 및 흡수 패드(300)를 각각 제조하는 단계; 및(b) manufacturing the sample pad 100, detection pad 200, and absorption pad 300, respectively; and (c) 상기 기판(400) 상에 상기 샘플 패드(100), 상기 검출 패드(200) 및 상기 흡수 패드(300)를 서로 접하도록 순서대로 형성하여 상기 샘플 패드(100), 상기 검출 패드(200) 및 상기 흡수 패드(300)가 선형으로 위치하는 마약류 분석 스트립(10)을 제조하는 단계;를 포함하고,(c) The sample pad 100, the detection pad 200, and the absorption pad 300 are sequentially formed on the substrate 400 so that the sample pad 100, the detection pad 200 are in contact with each other. ) and manufacturing a narcotic analysis strip (10) in which the absorbent pad (300) is positioned linearly, 상기 검출 패드(200)는 화학적 반응에 의해 색이 변화되는 마약탐지 시약(220)을 포함하는 것인, 마약류 검출용 측방유동 분석 스트립의 제조방법.The detection pad 200 includes a drug detection reagent 220 whose color changes by a chemical reaction. 제14항에 있어서,According to clause 14, 상기 마약탐지 시약(220)이 The drug detection reagent (220) is Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van Urk reagent 및 Vanillin reagent로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제1 마약탐지 시약(221);Dragendorff reagent, Bath Salts/MDPV reagent, Diazotization reagent, Dille-koppanyi reagent, Duquenois-Levine reagent, Ehrlich reagent, Fentanyl reagent, Froehde reagent, KN (Fast Blue B Salt) reagent, Libermann reagent, Mandelin reagent, Marquis reagent, Mecke A group consisting of reagent, Methamphetamine reagent, Methaqualone/PCP reagent, Mollies reagent, Morris reagent, Nitric acid reagent, Opiates reagent, p-DMAB reagent, Psilocybin reagent, Scott reagent, Sulfuric acid reagent, Talwin reagent, Van Urk reagent and Vanillin reagent. A first drug detection reagent (221) containing one or more types selected from; Hofmann's reagent 및 Valium reagent로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제2 마약탐지 시약(222);A second drug detection reagent (222) comprising at least one selected from the group consisting of Hofmann's reagent and Valium reagent; Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent 및 Zwikker reagent로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제3 마약탐지 시약(223); 및One selected from the group consisting of Ninhydrin reagent, PCP reagent, Bath Salts/Mephedrone reagent, Bzd reagent, Chen-Kao reagent, Cannabis reagent, folin reagent, Robadope reagent, Rohypnol reagent, Simon reagent, TBPE reagent, Zimmerman reagent and Zwikker reagent A third drug detection reagent (223) including the above; and Iodoplatinate reagent, Scott reagent 및 Marquis로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제4 마약탐지 시약(224);으로 이루어진 군으로부터 선택된 1종 이상을 포함하는 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립의 제조방법.A fourth drug detection reagent (224) containing at least one selected from the group consisting of Iodoplatinate reagent, Scott reagent and Marquis; of a lateral flow analysis strip for drug detection, characterized in that it contains at least one selected from the group consisting of Manufacturing method. 제15항에 있어서,According to clause 15, 상기 단계 (b)의 상기 검출 패드(200)를 제조하는 단계가 The step of manufacturing the detection pad 200 in step (b) is (1) 니트로셀룰로오스, 셀룰로오스, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제2 다공성 막(210)을 제공하는 단계;(1) providing a second porous membrane 210 comprising one or more selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper; (2) 상기 제1 마약탐지 시약(221)과 고체산 또는 비휘발성 산을 혼합하여 혼합물을 제조하는 단계; 및(2) preparing a mixture by mixing the first drug detection reagent 221 and a solid acid or non-volatile acid; and (3) 상기 제2 다공성 막(210) 상에 상기 혼합물을 도포 후 건조시켜 제1 마약탐지 시약(221)을 포함하는 검출 패드(200)를 제조하는 단계;를(3) manufacturing a detection pad 200 containing the first drug detection reagent 221 by applying the mixture on the second porous membrane 210 and drying it; 포함하는 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립의 제조방법.A method of manufacturing a lateral flow analysis strip for detecting narcotics, comprising: 제15항에 있어서,According to clause 15, 상기 단계 (b)의 상기 검출 패드(200)를 제조하는 단계가 The step of manufacturing the detection pad 200 in step (b) is (1') 니트로셀룰로오스, 셀룰로오스, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제2 다공성 막(210)을 제공하는 단계;(1') providing a second porous membrane 210 comprising at least one selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper; (2') 상기 제2 마약탐지 시약(222)과 고체염기 또는 비휘발성 염기를 혼합하여 혼합물을 제조하는 단계; 및(2') preparing a mixture by mixing the second drug detection reagent 222 with a solid base or non-volatile base; and (3') 상기 제2 다공성 막(210) 상에 상기 혼합물을 도포 후 건조시켜 제2 마약탐지 시약(222)을 포함하는 검출 패드(200)를 제조하는 단계;를(3') manufacturing a detection pad 200 containing a second drug detection reagent 222 by applying the mixture on the second porous membrane 210 and drying it; 포함하는 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립의 제조방법.A method of manufacturing a lateral flow analysis strip for detecting narcotics, comprising: 제15항에 있어서,According to clause 15, 상기 단계 (b)의 상기 검출 패드(200)를 제조하는 단계가 The step of manufacturing the detection pad 200 in step (b) is (1") 니트로셀룰로오스, 셀룰로오스, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제2 다공성 막(210)을 제공하는 단계;(1") providing a second porous membrane 210 comprising at least one member selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper; (2") 유기용매 및 물을 포함하는 용액에 상기 제3 마약탐지 시약(223)을 혼합하여 혼합물을 제조하는 단계; 및(2") preparing a mixture by mixing the third drug detection reagent 223 with a solution containing an organic solvent and water; and (3") 상기 제2 다공성 막(210) 상에 상기 혼합물을 도포 후 건조시켜 제3 마약탐지 시약(223)을 포함하는 검출 패드(200)를 제조하는 단계;를(3") manufacturing a detection pad 200 containing a third drug detection reagent 223 by applying the mixture on the second porous membrane 210 and drying it; 포함하는 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립의 제조방법.A method of manufacturing a lateral flow analysis strip for detecting narcotics, comprising: 제15항에 있어서,According to clause 15, 상기 단계 (b)의 상기 검출 패드(200)를 제조하는 단계가 The step of manufacturing the detection pad 200 in step (b) is (i) 니트로셀룰로오스, 셀룰로오스, 직조 메쉬(woven mesh), 폴리에테르설폰(PES) 및 크로마토그래피 페이퍼로 이루어진 군으로부터 선택된 1종 이상을 포함하는 제2 다공성 막(210)을 제공하는 단계;(i) providing a second porous membrane (210) comprising at least one selected from the group consisting of nitrocellulose, cellulose, woven mesh, polyethersulfone (PES), and chromatography paper; (ii) 물에 의해 희석된 제4 마약탐지 시약 전구체로부터 제4 마약탐지 시약(224)을 포함하는 용액을 제조하는 단계;(ii) preparing a solution containing the fourth drug detection reagent (224) from the fourth drug detection reagent precursor diluted with water; (iii) 상기 제2 다공성 막(210) 상에 상기 용액을 도포 후 건조시켜 제4 마약탐지 시약(224)을 포함하는 검출 패드(200)를 제조하는 단계;를(iii) manufacturing a detection pad 200 containing a fourth drug detection reagent 224 by applying the solution on the second porous membrane 210 and drying it; 포함하는 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립의 제조방법.A method of manufacturing a lateral flow analysis strip for detecting narcotics, comprising: 제19항에 있어서,According to clause 19, 상기 제4 마약탐지 시약 전구체가 물에 의해 2 내지 5배 희석된 것을 특징으로 하는 마약류 검출용 측방유동 분석 스트립의 제조방법.A method of manufacturing a lateral flow analysis strip for drug detection, characterized in that the fourth drug detection reagent precursor is diluted 2 to 5 times with water.
PCT/KR2023/002792 2022-04-29 2023-02-28 Lateral flow assay strip for detecting drugs using drug detection reagent, and manufacturing method therefor Ceased WO2023210944A1 (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR19990015344A (en) * 1997-08-05 1999-03-05 허일섭 Instant drug detection site and its manufacturing method
US20040151624A1 (en) * 2003-01-30 2004-08-05 Abe Erdman Apparatus and method for drug testing
US20140141519A1 (en) * 2012-11-21 2014-05-22 Jpp Chromatography Limited Novel Reagent and Method Using the Same
US20150017732A1 (en) * 2013-07-12 2015-01-15 Tsunghsueh Wu Colorimetric method to detect illicit drugs
JP2015535078A (en) * 2012-10-16 2015-12-07 ボディテックメド インコーポレイテッドＢｏｄｉｔｅｃｈｍｅｄ． Ｉｎｃ Side flow analysis strip with subpad and side flow analysis cartridge used therefor

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR19990015344A (en) * 1997-08-05 1999-03-05 허일섭 Instant drug detection site and its manufacturing method
US20040151624A1 (en) * 2003-01-30 2004-08-05 Abe Erdman Apparatus and method for drug testing
JP2015535078A (en) * 2012-10-16 2015-12-07 ボディテックメド インコーポレイテッドＢｏｄｉｔｅｃｈｍｅｄ． Ｉｎｃ Side flow analysis strip with subpad and side flow analysis cartridge used therefor
US20140141519A1 (en) * 2012-11-21 2014-05-22 Jpp Chromatography Limited Novel Reagent and Method Using the Same
US20150017732A1 (en) * 2013-07-12 2015-01-15 Tsunghsueh Wu Colorimetric method to detect illicit drugs

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