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WO2023138680A8 - Method for inducing cell differentiation - Google Patents

Method for inducing cell differentiation Download PDF

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Publication number
WO2023138680A8
WO2023138680A8 PCT/CN2023/073316 CN2023073316W WO2023138680A8 WO 2023138680 A8 WO2023138680 A8 WO 2023138680A8 CN 2023073316 W CN2023073316 W CN 2023073316W WO 2023138680 A8 WO2023138680 A8 WO 2023138680A8
Authority
WO
WIPO (PCT)
Prior art keywords
cells
hematopoietic
progenitor cells
pluripotent stem
cell differentiation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2023/073316
Other languages
French (fr)
Chinese (zh)
Other versions
WO2023138680A9 (en
WO2023138680A1 (en
Inventor
李宗海
贾倩倩
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carsgen Therapeutics Ltd
Original Assignee
Carsgen Therapeutics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carsgen Therapeutics Ltd filed Critical Carsgen Therapeutics Ltd
Priority to CN202380017256.0A priority Critical patent/CN118829724A/en
Publication of WO2023138680A1 publication Critical patent/WO2023138680A1/en
Publication of WO2023138680A9 publication Critical patent/WO2023138680A9/en
Publication of WO2023138680A8 publication Critical patent/WO2023138680A8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Provided are a culture platform, a cell culture medium, and a method for differentiating pluripotent stem cells into a hematopoietic lineage. A pluripotent stem cell-derived hematopoietic lineage produced by using the present culture platform and method can be produced by means of a monolayer culture without serum or trophoblasts, and without forming embryoid bodies. The pluripotent stem cell-derived hematopoietic lineage comprises hematopoietic endothelial cells, hematopoietic stem cells, hematopoietic pluripotent progenitor cells, hematopoietic progenitor cells, T progenitor cells, NK progenitor cells, T cells, and NK cells.
PCT/CN2023/073316 2022-01-20 2023-01-20 Method for inducing cell differentiation Ceased WO2023138680A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202380017256.0A CN118829724A (en) 2022-01-20 2023-01-20 Methods for inducing cell differentiation

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
CN202210066079 2022-01-20
CN202210066079.2 2022-01-20
CN202210935441.5 2022-08-03
CN202210935441 2022-08-03
CN202210932654 2022-08-04
CN202210932654.2 2022-08-04

Publications (3)

Publication Number Publication Date
WO2023138680A1 WO2023138680A1 (en) 2023-07-27
WO2023138680A9 WO2023138680A9 (en) 2023-08-31
WO2023138680A8 true WO2023138680A8 (en) 2023-11-02

Family

ID=87347876

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2023/073316 Ceased WO2023138680A1 (en) 2022-01-20 2023-01-20 Method for inducing cell differentiation

Country Status (2)

Country Link
CN (1) CN118829724A (en)
WO (1) WO2023138680A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117448269B (en) * 2023-10-25 2025-06-17 中国科学院香港创新研究院再生医学与健康创新中心 A method for inducing rapid differentiation of human pluripotent stem cells into macrophages and its application
CN118421559B (en) * 2024-07-04 2024-09-27 成都赛济元生物医药有限公司 Small molecule combination, kit and method for directed differentiation of lymphohematopoietic precursor cells
CN120137891B (en) * 2025-03-13 2025-10-17 广东恒达嘉生物科技有限公司 Stem cell exosomes and application thereof in preparation of anti-aging and beautifying cosmetics or drugs
CN120173879A (en) * 2025-05-13 2025-06-20 细胞生态海河实验室 A culture medium composition for differentiating pluripotent stem cells into hematopoietic stem and progenitor cells, and its use method and application

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114058582A (en) * 2015-01-26 2022-02-18 菲特治疗公司 Methods and compositions for inducing hematopoietic cell differentiation
EP3371301A4 (en) * 2015-11-04 2019-06-26 Fate Therapeutics, Inc. METHODS AND COMPOSITIONS FOR INDUCING THE DIFFERENTIATION OF HEMATOPOIETIC CELLS
CN113462642A (en) * 2021-08-12 2021-10-01 呈诺再生医学科技(珠海横琴新区)有限公司 Rapid induced differentiation method and kit of mesenchymal stem cells and application of kit

Also Published As

Publication number Publication date
WO2023138680A9 (en) 2023-08-31
CN118829724A (en) 2024-10-22
WO2023138680A1 (en) 2023-07-27

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