WO2023127055A1 - Concentrated natural hot spring water and method for producing same - Google Patents

Abstract

Provided is a liquid of highly absorbable minerals, the liquid being able to supply necessary amounts of minerals containing metal elements that are essential trace elements for living organisms such as animals and plants.　A metal-containing acidic solution comprises a natural hot spring component and a mineral-containing material of an inorganic substance treated with an inorganic acid, and is characterized by comprising an organic substance and an inorganic substance in hot spring water and being capable of oxidizing the organic and inorganic substances.

Description

Natural hot spring concentrate and its production method

In the present invention, by adding mineral-containing ore to a natural hot spring and treating it with an inorganic acid, the hot spring components contained in the natural hot spring are enhanced, resulting in a warm bathing effect, replenishment of mineral nutrients in the body tissue, and elimination of bacteria in the natural hot spring. Bacteria, sterilization, deodorization, deodorization, sleep induction by hot bath effect in a state of low water temperature, improvement of taste and nutritional supplementation by adding to various foods as a seasoning.

Natural minerals contain large amounts of various metal elements as minerals, ranging from major metals such as calcium, silica, and iron to trace metals. These minerals are generally useful for living organisms such as animals and plants. It is an essential trace element.

Conventionally, these metal elements are generally provided in the form of natural hot springs, metals, or salts. This does not necessarily mean that it will be easily absorbed by the living body, and its possible absorption rate was only low.

Japanese Patent No. 3872501

Physician's Desk Reference, NPPDR No.18: 676, 1997

The inventor of the present invention, as a result of extensive research aimed at enhancing minerals, which are active ingredients contained in natural hot springs, found that when mineral acids are applied to mineral-containing ores, many minerals are eluted in a form that is easily absorbed by the body. And this mineral content not only strengthens the hot spring ingredients, but also enhances the effect of hot baths, balances mineral ingredients in the body, kills bacteria in the bath, deodorizes, deodorizes, and the water temperature is low. The inventors have found that it is useful for inducing sleep due to the warm bath effect in a state of low burden and can be used as a seasoning by adding it to various foods, and have completed the present invention. In particular, Non-Patent Document 1 shows that this mineral is absorbed by intraoral or sublingual spraying. According to FIG. 1, the absorption rate by oral or sublingual spray is 95%, while intravenous injection is the highest, 99%. This is higher than tablets at 10%, capsules at 20%, transdermal patches at 45%, sublingual fluid at 50% and intramuscular injection at 80%.

Accordingly, an object of the present invention is to provide minerals composed of metal elements, which are essential trace elements for living organisms such as animals and plants, in the form of liquids that can be ingested in amounts necessary for living organisms and have a high possible absorbability. That's what it is.

Another object of the present invention is to produce safe cleaning products that are useful for cleaning solutions and the like in hot bath facilities and restaurants, and that do not use chemical substances that are harmful to the human body.

A further object of the present invention is to provide a natural hot spring concentrate that produces hair care products using minerals and has the property of oxidizing organic and inorganic substances.

A further object of the present invention is to provide a natural hot spring concentrate that produces oral care products using minerals and has the property of oxidizing organic and inorganic substances.

A further object of the present invention is to provide a natural hot spring concentrate that produces body care products using minerals and has the property of oxidizing organic and inorganic substances.

In order to achieve the object of the present invention, the natural hot spring concentrate for oxidizing organic matter in water and inorganic matter according to the present invention is a metal-containing acidic solution containing natural hot spring components and inorganic acid-treated minerals containing minerals. It is characterized by having the property of containing and oxidizing organic matter and inorganic matter in the hot spring liquid.

The natural hot spring concentrate contains 1-20g/liter of iron, 1-10g/liter of magnesium and 1-10g/liter of potassium as main components, and its specific gravity is 1.1-1.5 and pH is 0. .1 to 2.0.

Natural hot spring concentrate contains a large amount of iron, magnesium and potassium as metal components, and a large amount of metal consisting of phosphorus, titanium, calcium, manganese, sodium, silica, cobalt, lithium, copper, nickel, zinc and barium. containing parts and free of arsenic, lead, mercury and chromium.

In addition, the virus inactivating agent according to the present invention is a metal-containing acidic solution containing natural hot spring ingredients and inorganic acid-treated minerals containing minerals, and contains organic substances and inorganic substances in the hot spring liquid and oxidizes them. It consists of a natural hot spring concentrate for oxidizing organic matter in water and inorganic matter, characterized by having the property of

Furthermore, the virus inactivating agent contains 1-20 g/liter iron, 1-10 g/liter magnesium and 1-10 g/liter potassium as main components, and its specific gravity is 1.1-1.5, pH is 0.1 to 2.0.

In addition, the virus inactivating agent contains large amounts of iron, magnesium and potassium as metal components, and consists of phosphorus, titanium, calcium, manganese, sodium, silica, cobalt, lithium, copper, nickel, zinc and barium. Contains most of the metals and is free of arsenic, lead, mercury and chromium.

Next, the liquid fertilizer composed of natural hot spring concentrates for oxidizing organic substances in water and inorganic substances according to the present invention is a metal-containing acidic solution containing natural hot spring components and inorganic acid-treated minerals containing minerals. It is characterized by having the property of containing and oxidizing both organic matter and inorganic matter.

Further, the liquid fertilizer contains 1-20 g/liter of iron, 1-10 g/liter of magnesium and 1-10 g/liter of potassium as main components, and has a specific gravity of 1.1-1.5 and a pH of 0.5. 1 to 2.0.

This liquid fertilizer contains large amounts of iron, magnesium and potassium as metal components, and most of the metals consisting of phosphorus, titanium, calcium, manganese, sodium, silica, cobalt, lithium, copper, nickel, zinc and barium. Contains and does not contain arsenic, lead, mercury or chromium.

In addition, the natural hot spring concentrated drinking water according to the present invention is a metal-containing acidic solution containing natural hot spring ingredients and inorganic acid-treated minerals containing minerals, and contains organic and inorganic substances in the hot spring liquid. It is characterized by having the property of being oxidized.

Furthermore, the natural hot spring concentrated drinking water contains 1-20 g/liter of iron, 1-10 g/liter of magnesium and 1-10 g/liter of potassium as main components, and its specific gravity is 1.1-1.5, The pH is 0.1-2.0.

In addition, natural hot spring concentrated drinking water contains a large amount of iron, magnesium and potassium as metal components, and contains phosphorus, titanium, calcium, manganese, sodium, silica, cobalt, lithium, copper, nickel, zinc and barium. contains most of the most common metals and does not contain arsenic, lead, mercury or chromium.

Further, the natural hot spring concentrate according to the present invention is a metal-containing acidic solution containing natural hot spring ingredients and inorganic acid-treated minerals containing minerals, and contains organic substances and inorganic substances in the hot spring liquid and oxidizes them. It is characterized by having the characteristic of

Furthermore, the natural hot spring concentrate contains 1 to 20 g/liter iron, 1 to 10 g/liter magnesium and 1 to 10 g/liter potassium as main components, and its specific gravity is 1.1 to 1.5, pH is 0.1 to 2.0.

In addition, the natural hot spring concentrate contains a large amount of iron, magnesium and potassium as metal components, and consists of phosphorus, titanium, calcium, manganese, sodium, silica, cobalt, lithium, copper, nickel, zinc and barium. Contains most of the metals and is free of arsenic, lead, mercury and chromium.

As described in detail above, the natural hot spring concentrate for oxidizing organic matter and inorganic matter in water according to the present invention has the effect of promoting the growth of animals, plants, and fish and shellfish, and also has the property of generating OH radicals and oxidizing organic matter. Therefore, it has the effect of reducing organic substances and bacteria in sewage and service water, especially for cleaning various sewage or service water, antibacterial bacteria, deodorizing, deodorizing, removing blue-green algae, sludge treatment, plant It can be widely used as an active ingredient of various additives in the agricultural and horticultural fields, the fisheries field, the wastewater treatment field, the water supply business, etc., mainly for the promotion of seed germination and the growth promotion of animals and plants.

In addition, drinking natural hot spring concentrated drinking water affects blood sugar levels in the body. In diabetics, especially non-insulin dependent diabetics, blood sugar levels begin to drop immediately upon ingestion of the drinking water of the present invention. Diabetes is associated with insulin production, receptor problems, numerous sugar metabolisms and myriad enzymes. Natural hot spring concentrate (registered trademark: craft hot spring) has a multi-characteristic effect that can be applied to any event by replenishing metal ions.

It is a graph of the inhalation rate according to the method of internal intake. 1 is a block chart of a method for producing a natural hot spring concentrate according to the present invention. 4 is a measurement chart of the natural hot spring concentrate according to the present invention by oligoscan. 4 is a measurement chart of the natural hot spring concentrate according to the present invention by oligoscan. 4 is a measurement chart of the natural hot spring concentrate according to the present invention by oligoscan. 4 is a measurement chart of the natural hot spring concentrate according to the present invention by oligoscan. 4 is a measurement chart of the natural hot spring concentrate according to the present invention by oligoscan. 4 is a measurement chart of the natural hot spring concentrate according to the present invention by oligoscan. It is an influenza infection titer measurement graph of the natural hot spring concentrate according to the present invention. Fig. 2 is a comparison photograph of rice grown with liquid fertilizer from the natural hot spring concentrate according to the present invention and normal rice. It is a graph regarding the inactivation evaluation by the natural hot spring concentrated inactivating agent according to the present invention. It is a graph regarding the inactivation evaluation by the natural hot spring concentrated inactivating agent according to the present invention. It is a graph regarding the inactivation evaluation by the natural hot spring concentrated inactivating agent according to the present invention. It is a graph regarding the inactivation evaluation by the natural hot spring concentrated inactivating agent according to the present invention.

The present invention, which achieves such objects, consists of the following technical means (1) to (3).

(1) A metal-containing acidic solution made of corrosive minerals treated with an inorganic acid, which, when added to a natural hot spring, has the property of oxidizing organic and inorganic substances in the aqueous solution. Natural hot spring inorganic blend for organic and inorganic oxidation treatment.

Here, the natural hot spring concentrate according to the present invention is preferably sodium: 100 ml/L to 200 ml/L, magnesium: 2000 ml/L to 4000 ml/L, silica: 10 ml/L to 40 ml/L, phosphorus: 100 ml/L. L~400ml/L, potassium: 1000ml/L~4000ml/L, calcium: 100ml/L~500ml/L, titanium: 500ml/L~1000ml/L, chromium: 3ml/L~20ml/L, manganese: 50ml/L L~200ml/L, Iron: 5000ml/L~30000ml/L, Cobalt: 2ml/L~10ml/L, Copper: 2ml/L~20ml/L, Zinc: 10ml/L~30ml/L, Germanium: 10ml/L L or less, selenium: 1 ml/L to 10 ml/L, molybdenum: 5 ml/L or less.

The natural hot spring concentrate according to the present invention is more preferably sodium: 170 ml/L, magnesium: 3000 ml/L, silica: 28 ml/L, phosphorus: 300 ml/L, potassium: 2500 ml/L, calcium: 400 ml/L. , Titanium: 860ml/L, Chromium: 8ml/L, Manganese: 140ml/L, Iron: 10000ml/L, Cobalt: 4ml/L, Copper: 8ml/L, Zinc: 17ml/L, Germanium: 1ml/L or less, Selenium: 5ml/L Molybdenum: 0.5ml/L or less is desirable.

(2) Contains 1 to 20 g/liter of iron, 1 to 10 g/liter of magnesium and 1 to 10 g/liter of potassium as main components, and has a specific gravity of 1.1 to 1.5 and a pH of 0.1 to 0.1. 2.0 of the natural hot spring inorganic blend for the inorganic substance oxidation treatment.

(3) Contains a large amount of iron, magnesium and potassium as metal components, and contains most of the metals consisting of phosphorus, titanium, calcium, manganese, sodium, silica, cobalt, lithium, copper, nickel, zinc and barium. , and the natural hot spring concentrate according to the above (1), which does not contain arsenic, lead, mercury, or chromium.

The natural hot spring concentrate of the present invention is obtained by using corrosive minerals such as biotite, pretreating it if necessary, reacting it with a mineral acid, and separating it. In addition, pretreatment of biotite is adopted as necessary in order to allow mineral acid to act efficiently, and the pretreatment is, for example, pulverizing the weathered soil of biotite into an appropriate size. performed by

For the treatment with mineral acid after the pretreatment, the mineral acid solution should be applied to the weathered biotite soil to perform elution treatment. Sulfuric acid is used as the mineral acid. Mineral acid treatment is preferably performed at a heating temperature if necessary, and is continued until necessary mineral components are eluted from the biotite.

Furthermore, the mineral acid-treated product is separated into a liquid and a solid by solid-liquid separation. As a solid-liquid separation means, for example, a filter press or the like is used, and if necessary, organic substances in water having the action of further separating and purifying them to oxidize organic substances and inorganic substances and natural hot spring concentrates for oxidizing inorganic substances are fractionated. Specifically, the manufacturing process is shown in FIG.

The first step of the manufacturing process of the present invention is the step of excavating the weathered biotite soil as the original soil (S2).

The second step is to accumulate the mineral acid in the tank to dissolve the original soil (S4). Mineral acids include hydrochloric acid, nitric acid, sulfuric acid, acetic acid, and carbonic acid.

Subsequently, in the third step, the mineral acid is diluted with a dilution layer (S6). Although it is diluted with pure water, it may also be diluted with hot spring diving here.

In the fourth step, the raw soil is stirred in the raw soil hopper with diluted acid (S8).

In the fifth step, the raw soil that has been stirred with acid is separated into finer-grained raw soil by the raw soil separation equipment to be used as raw materials (S10).

In the sixth step, the sorted raw materials are further stored in a raw material hopper, and dropped from a bottom-opening funnel-shaped mouth to be taken out (S12).

In the seventh step, the raw material taken from the raw material hopper is transferred to the extraction tank (S14).

In the eighth step, the classifier separates gravel and rocks with a large grain size of 4 mm or more (S16). The classified pebbles and rocks having a grain size larger than the standard size of 4 mm or more are discarded as residual rocks (S18). Sand, silt, minerals and moisture smaller than the standard are stored in the relay tank (S20).

In the ninth step, small sand, silt, minerals and moisture in the relay tank are filtered by a filter press, and small sand and silt are discarded as residual soil (S24).

In the tenth step, the minerals, moisture, and residue are deposited in the filtrate tank, and the mineral mixture of minerals and water is stored as the supernatant (S26).

In the eleventh step, the hot spring water containing the components unique to the hot spring area, taken from the source of the specific hot spring area, is stored in advance in the hot spring water tank (S30). For example, if the hot spring is Kusatsu hot spring, the source water of Kusatsu hot spring water is stored.

In the twelfth step, the mineral mixture of minerals and water stored in the filtrate tank and the hot spring water are agitated and mixed in the mixing tank to produce a natural hot spring concentrate in the mixing tank (S28).

In the thirteenth step, the natural hot spring concentrate in the mixing tank is stored in containers such as bottles, PET bottles, cans, plastic bags, etc. depending on the application and commercialized (S32).

The properties of the natural hot spring concentrate of the present invention thus obtained are as follows.
1. Hot spring component content: Contains 20% or more of mineral content in terms of salt content.
2. Hot spring component breakdown: (1) Contains a large amount of iron, magnesium and potassium.
(2) Contains most of phosphorus, titanium, calcium, manganese, sodium, silica, cobalt, lithium, copper, nickel, zinc and barium.
(3) Does not contain arsenic, lead, mercury or chromium.

3. Specific gravity: 1.1-1.54. pH: 0.1-2.05. Function: ■ By adding hot spring minerals to natural hot springs, the quality of the hot springs is greatly improved, and effects such as heat retention, moisturizing, lower back pain, and rough skin can be seen.
(Example 1)

The natural hot spring concentrate formed by the above method was used as a hot spring mist in a hot spring mist suction facility shown in patent No. 6523960, which was invented separately by the inventor. 3-8. All of them were measured using Oligoscan equipment using Raman spectroscopy in a non-invasive and short-time measurement of harmful heavy metals and essential/reference minerals manufactured by Oligoscan. Measurement targets were four palms of each subject. The measurement light is a UV/tungsten lamp with a wavelength of 380 nm to 730 nm. Regarding the intake of essential minerals, when not using the natural hot spring concentrate (Fig. 3), when using the natural hot spring concentrate for 6 months (Fig. 4). After using the natural hot spring concentrate for 6 months (Fig. 6), the immune strength of each subject was measured by 4 palms when not using the natural hot spring concentrate (Fig. 7), and after using the natural hot spring concentrate for 6 months (Fig. 8). Each component of the results of measurement using an oligoscan device from the factory is shown. Measurement results are displayed as measured values (unit: μg/mg Tissues) and graphs. In the graph of essential and reference minerals, based on the database, the standard range of ±1SD is indicated as the green zone, ±1SD<±2SD as the yellow zone, and ±2SD<red zone.

Comparing Figures 3 and 4 for the intake of essential minerals, the absorption of major minerals by using the natural hot spring concentrate increased potassium by 265%, calcium by 25%, and sodium by 6%. It can be seen that sulfur also increases by 0.6%.

The trace minerals are 198% copper, 139% molybdenum, 158% vanadium, 50% zinc, 31% chromium, 25% cobalt, 11% iodine, 10% iron, and 9% boron. increased by .8% each.

Comparing Figures 5 and 6 for harmful mineral emissions, barium decreased by 72%, thallium decreased by 66%, thorium decreased by 65%, arsenic decreased by 62%, bismuth decreased by 49%, and antimony decreased by 28%. % reduction, 26% reduction in cadmium, 22% reduction in mercury and 2.5% reduction in beryllium. It became clear that 9 types of harmful minerals were reduced.

7 and 8, as potential issues, total heavy metal non-toxicity improved from 75% requiring caution to 65% within the acceptable range, acidosis/acidosis improved from 79% requiring caution to 33% good, and diabetes Predisposition improved from acceptable 50% to good 27%, and allergy risk improved from acceptable 53% to good 33%.

In terms of physiological functions, enzyme status improved from 21% requiring caution to 97% good, green digestive organs improved from 25% requiring caution to 89% good, and metabolism improved from 27% requiring caution to 50% acceptable. Immune system improved from 21% needing caution to 88% good Cognitive function improved from 33% needing caution to 79% good Hormone status improved from 33% needing caution to 79% good Tissue repair improved from acceptable 50% to good 74%; emotional state improved from caution 27% to good 75%; cardiovascular system improved from caution 28% to good 72%; nervous system caution Improved from 32% to good 87%. From the above, potential problems and physiological functions are totally improved by the natural hot spring concentrate according to the present invention.
(Example 2)

A second embodiment using the natural hot spring concentrate formed by the above method will be described. A virus inactivation test was conducted using a natural hot spring concentrate. Using a 500 ppm aqueous solution of natural hot spring concentrate and a phosphate buffer (PBS(-)) as a test control, the inactivation effect on influenza virus was evaluated. The virus strain used was Influenza virus H1N1 A/PR/8/34 (ATCC VR-1469). In addition, MDCK cells (canine kidney cells) (ATCC CCL-34) were used as host cells.

The test method was to first add 0.01 mL of natural hot spring concentrate to 0.99 mL of purified water to create a 10000 ppm aqueous solution. Further, 0.15 mL of this natural hot spring concentrate 10000 ppm aqueous solution was added to 2.85 mL of purified water to prepare a 500 ppm aqueous solution.

After dispensing 1.08 mL of a 500 ppm aqueous solution of natural hot spring concentrate into a tube, 0.12 mL of an influenza solution adjusted to 5-10 x 105 pfu/ml using phosphate buffer (PBS) was mixed and stirred to obtain the test solution.

The test solution was allowed to stand at room temperature for reaction. 0.12 ml of the solution was collected from the test solution at predetermined time intervals (immediately, 1 minute, 5 minutes) and mixed with 1.08 ml of Dulbecco's modified Eagle's medium containing 0.2% bovine serum (FBS-DMEM medium). Dilution with 0.2% FBS-DMEM medium was repeated multiple times to prepare a 10-fold serial dilution series. Each 1 ml/WELL of the 10-fold serial dilution series was dropped onto the host cells prepared in advance, and infected at 37° C., 5% CO ₂ for 1 hour.

After virus infection, the cell supernatant was replaced with a 0.8% oxoid agar solution and cultured for 2 days at 37°C and 5% _CO2 . After visually confirming the formation of plaques, the cells were fixed with a 5% glutaraldehyde solution, stained with methylene blue, and the virus infectivity was measured based on the number of plaques formed.

This test was conducted twice, and based on the average value of the two times, the inactivation rate with a 500 ppm aqueous solution of natural hot spring concentrate was evaluated.

As shown in Table 1 and Figure 9, the phosphate buffer solution (PBS(-)) showed no change over time in the influenza infection titer, whereas the 500 ppm aqueous solution of the natural hot spring concentrate showed influenza infection in about 30 seconds. The infectious titer has dropped sharply to less than 10 pfu/ml. This is also clear from the table.

Therefore, the inactivating effect of the 500 ppm aqueous solution of the natural hot spring concentrate on the influenza virus was evaluated. The influenza virus used was the H1N1 serotype A/PR/8/34 strain. A 500 ppm natural hot spring concentrate aqueous solution was confirmed to have a virus inactivation effect of 99.988% or more immediately after the action.
(Example 3)

About natural hot spring concentrated liquid fertilizer

The natural hot spring concentrated liquid fertilizer functions as a photosynthesis-enhancing material rather than a fertilizer. Increases crop photosynthesis by 20-30%, mainly through foliar application. Rice seed disinfection. An example of use in raising seedlings will be described. The cultivar is Koshihikari, and 500-fold diluted natural hot spring concentrated liquid fertilizer is used for paddy disinfection, germinated for 3 days before sowing, immersed, and sprayed with 2000-fold hydrogen surface three times after germination.

By cultivating the natural hot spring concentrated liquid fertilizer under the above conditions, the germination rate is extremely good. In addition, there is no generation of poorly grown seedlings called so-called stupid seedlings. In addition, it was possible to grow without using any conventional seed disinfectant. Furthermore, the start of epiphytic growth immediately after rice planting was earlier than the conventional start date by 4 to 5 days. Furthermore, natural hot spring concentrated liquid fertilizer can be used as dilution water for other liquid fertilizers. Fig. 10 shows half-cropping of seedlings before shipment. The half-cropping of seedlings using the conventional method on the right side, and the half-cropping of seedlings using the natural hot spring concentrated liquid fertilizer on the left side clogged the internodes, resulting in thicker stems and better root tension.
(Example 4)

Next, the usefulness of the natural hot spring concentrate of the present invention will be described.
1. Use in the field of increasing the amount of natural hot spring ingredients. For example, by diluting a 50-5,000 ppm aqueous solution to about 0.1 ml/L, the natural hot spring concentrate of the present invention is converted to a hot spring rich in mineral ingredients to keep warm and moisturize. It is possible to increase the effect and replenish minerals by permeating the water-solubilized minerals through the skin. This makes it possible to treat hot springs anywhere without mining or transporting hot springs.
(Example 5)

（実施例６） Use of Natural Hot Springs in the Beverage Field By adding the natural hot spring concentrate of the present invention to drinking water or the like, it is possible to generate hot spring water containing high-quality multi-minerals, thereby resolving the lack of minerals in modern people. Over the past five years, it has been found that depending on the physical condition of the day, it may taste sour, bitter, or salty like blood. It is speculated that this is due to the lack of sublingual mucosa, that is, the fact that the body tries to absorb the minerals that it needs first, and this appears as a change in taste. In fact, immediate effects were observed for hangovers, constipation, and the like.

(Example 6)

Use of natural hot springs in the field of seasonings By adding the natural hot spring concentrate of the present invention as a seasoning to various dishes, it is possible to (1) soften meat, and (2) use it in dressings, etc. (3) tightening the taste by adding sourness; and (4) eliminating the fishy smell of fish and the like.
(Example 7)

Use in the cleaning field of natural hot springs Sterilization and cleaning can be performed by spraying about 0.1-100 ml/m ² of a 50-5,000 ppm aqueous solution of the natural hot spring concentrate of the present invention. It is possible to develop products that are safe for both the environment and the human body by switching from the chlorine-based cleaning and deodorizing materials that have been used in the past.
(Example 8)

Use of natural hot springs in the field of cosmetics By diluting the natural hot spring concentrate of the present invention into a 50-5,000 ppm aqueous solution and spraying it on the hair, face and body, the minerals permeate the hair, reducing damage to the hair, creating volume, It is possible to keep the skin smooth.
(Example 9)

Use in the field of deodorization and deodorization Bad odors can be removed by spraying the natural hot spring concentrate of the present invention, for example, about 0.1 to 100 ml/m ² of a 50 to 5,000 ppm aqueous solution against the odor source. can. The deodorant and deodorant composition containing the natural hot spring concentrate of the present invention as an active ingredient is in the form of solution, solid, aerosol, etc., and is used to prevent bad odors from human waste itself, toilets, garbage disposal sites, poultry and pig farms, etc. In addition, it can be widely used for deodorizing refrigerators, shoeboxes, pet breeding houses, etc., and for preventing body odors such as bad breath and armpits.
(Example 10)

Use in the field of culturing and accelerating the growth of microorganisms and plants 　The natural hot spring concentrate of the present invention can be diluted to a 50-5,000ppm aqueous solution, applied to the culture of microorganisms and plants, soaked, and sprayed to accelerate growth. . In particular, it has an excellent effect in hydroponics. Also, it becomes possible to increase the amount of minerals in the tissue.

Microorganisms for which the natural hot spring concentrate of the present invention can be advantageously used include euglena, chlorella, koji enzyme, cucumber, tomato, eggplant, green pepper, green onion, and broccoli, regardless of hydroponics, alley cultivation, greenhouse cultivation, or soil cultivation. , peas, kidney beans, burdock, radish sprouts, mitsuba, spinach, green onions, strawberries, watermelons, melons, and other vegetables; fruit trees; Chrysanthemum, Rose, Denphale, Oncidium, Phalaenopsis, Bougainvillea, Black tree, Monstera, Hibiscus, Arecacia, Golden Banyan, Happiness Tree, Carnation, Gerbera, Cyclamen, Cineraria, Julian, Begonia, Stock, Rapeseed flowers, etc. ; turf, leaf tobacco, tea, shiitake mushrooms, and the like. Furthermore, it is useful for preserving the freshness of vegetables and fruits and preserving the life of flowering plants.
(Example 11)

Use in aquaculture By adding about 20 to 500 ppm of the natural hot spring concentrate of the present invention to a water tank, the feeding of farmed fish and shellfish is improved, and effects such as promotion of growth are exhibited. In addition, microscopic animals such as rotifers and daphnids that serve as live food proliferate, and aquatic plants grow rapidly.

Examples of cultured fish whose growth is promoted by using the natural hot spring concentrate of the present invention include coho salmon, king salmon, yamame trout, rainbow trout, dace, haya, tanago, carp, crucian carp, eel, catfish, and loach. freshwater cultured fish such as squid; seawater cultured fish such as medina, rockfish, soy, flounder, sea bream, and filefish; tropical fish such as guppy, oscar, angelfish, and neon tetra; shellfish such as pearl oysters, oysters, and scallops.
(Example 12)

Application in the field of sewage treatment By adding about 100 to 50,000 ppm of the natural hot spring concentrate of the present invention to sewage, organic substances contained in sewage are decomposed, precipitated, aggregated, and sedimented, and the sewage is converted into clean water. becomes possible. Examples of sewage that can be treated with the natural hot spring concentrate of the present invention include marine processing wastewater, livestock manure, poultry farming wastewater, human manure, and chemical industry wastewater.
(Example 13)

Use in the field of water purification By adding about 20 to 500 ppm of the natural hot spring concentrate of the present invention to tap water, it is possible to remove the precipitation of soluble organic matter, sodium hypochlorite itself, and trihalomethanes produced as by-products during chlorine sterilization. can be decomposed and precipitated to be detoxified and deodorized, and further minerals are replenished, so that tap water can be changed into sterile mineral water. In addition, by adding the natural hot spring concentrate of the present invention to lake water and river water, organic substances and microorganisms contained in these waters are decomposed, precipitated, aggregated, and filtered to make safe drinking water. can.
(Example 14)

Use in the field of sterilization and disinfection By adding 100 to 1,000 ppm of the natural hot spring concentrate of the present invention to water, it is possible to make an aqueous solution with antibacterial action, so it can be used, for example, in hot water such as baths, hot springs, and pools. It is useful in processing and is applicable to all applications that currently use sodium hypochlorite for disinfection.
(Example 15)

Biotite was pulverized and sieved to powder of 4 mesh or less. 1 m 3 of 25-50% sulfuric acid was added to 1 m 3 of this powder and stirred at 80° C. for 4 hours at 100 rpm (rotations per minute) and then at 50 rpm for 1 hour. Then, this mixture was subjected to a filter press for solid-liquid separation and purification to obtain 1.5 m ³ of a liquid having the action of generating OH radicals and oxidizing organic matter. The resulting natural hot spring concentrate had a pH of 0.2.

As is clear from the results in Table 3, the natural hot spring concentrate of the present invention contained a large amount of various minerals.
(Example 16)

Vermiculite, which is a weathered granite, was pulverized and sieved to powder of 4 mesh or less. 1 m ³ of 25-50% sulfuric acid was added to 1 m ³ of this powder and stirred at 80° C. for 4 hours at 100 rpm (rotations per minute) and then at 50 rpm for 1 hour. Then, this mixture was subjected to a filter press for solid-liquid separation and purification to obtain 1.5 m ³ of a liquid having the action of generating OH radicals and oxidizing organic substances.

As is clear from these results, the half-value width of tap water was 132 Hz, while the half-value width of tap water after adding the natural hot spring concentrate was 74 Hz. It was shown that At present, water with smaller clusters is said to be of better quality, more delicious, more active, and more beneficial to living organisms.
(Example 17)

Use for farmed fish: The natural hot spring concentrate of the present invention was added at a concentration of 100 ppm to the water used for farming eels (Imizu Shizen), and the effect was investigated. A culture tank used for cultivating eels is 250 tons, and 32,000 juvenile eels were bred in this tank. The breeding method was feeding in exactly the same way as before.

As a result, juvenile fish changed to adult fish in 160 to 180 days after the start of breeding, and the growth period was greatly shortened compared to the conventional method. Moreover, during the breeding period, the pH was stable at 6 to 6.7, the amount of dissolved oxygen (DO) was large, and the amount of microorganisms was about 1.5×10 ³ on average. Furthermore, the chromaticity of the breeding water was about 1.4 to 1.8, and the bottom was now visible, whereas the transparency was about 10 cm in the past. Furthermore, the amount of calcium ions in breeding water was reduced from about 2,600 ppm to 550-580 ppm. No disease was observed during the breeding period, and the survival rate was almost 100%.
(Example 18)

The natural hot spring concentrate obtained in Example 14 was dissolved in water to a concentration of 500 ppm, and this was filled in an aerosol container together with a propellant gas (LPG) to prepare a deodorant spray. When this deodorizing spray was sprayed on an ordinary household refrigerator, the putrid smell (bad smell) in the refrigerator disappeared immediately. The effect lasted until the next day. Also, when this deodorizing spray was sprayed on a toilet with a bad smell, the bad smell disappeared and the effect lasted for 3 hours. In addition, when this deodorant spray was sprayed on the mouth of a person suffering from bad breath caused by alveolar pyorrhea, the bad breath disappeared and the effect continued for 30 minutes. Furthermore, when this deodorizing spray was sprayed on a dog house with animal odor, the animal odor disappeared and the effect lasted for 6 hours.

In addition, we selected bird cages with fecal odors, infant diapers, circulating toilets attached to trains, waste disposal sites, and tropical fish tanks as odor sources, and treated them in the same way, and the same results were obtained. .
(Example 19)

For the water of Koya Pond (Itami City, Hyogo Prefecture) where the occurrence of blue-green algae is recognized, by using the same natural hot spring concentrate as the algae-removing agent obtained in Example 14, it is possible to use it as domestic water and drinking water. We examined whether it is possible to purify the

In the test, 10 tons of pond water was pumped into a rectangular 10-ton water tank, which was made of iron plate and had a transparent acrylic window with a diameter of about 30 cm attached to the side so that conditions inside the tank could be observed during work. , Install an automatic chemical addition device and a floating sludge collection device to this, treat the pond water with the same natural hot spring concentrate as obtained in Example 1, and remove COD, SS, total phosphorus, total nitrogen and blue-green algae. The change in the amount of various algae contained was examined. Q323
(Example 20)

Sludge accumulated over several decades in a pond of a certain shrine was sucked up by a slurry pump and collected in a 200-liter polyethylene tank. To this, 200 ml of an inorganic acid-treated product derived from vermiculite (trade name: Craft Onsen, manufactured by Shimanishi Kaken Co., Ltd.) was added, stirred, and allowed to stand for 30 minutes. After 30 minutes, the slurry separated into 100 liters of supernatant liquid and 100 liters of concentrated slurry. The supernatant water was siphoned off to obtain a concentrated sludge slurry (water content 30%). Since the supernatant had a pH of 6.5, it was directly refluxed into the pond. A slurry of concentrated sludge could be mixed with fertilizer, dried and used as a soil conditioner.
(Example 21)

Next, 100 ppm (10 g on a dry basis) of a polymer flocculant (neutral anionic, ACOFLOC A-110, manufactured by Mitsui Cyanamit Co., Ltd.) was added to the concentrated sludge slurry obtained in Example 20, and the mixture was stirred. A slurry of concentrated sludge was agglomerated into gelatin.

Next, another 200-liter plastic tank (with a grid placed 20 cm higher than the bottom and a synthetic fiber filter cloth spread over it) was prepared, and the above-mentioned gelatinous and agglomerated slurry was put into it. After leaving it for 16 hours, it was filtered and dehydrated. As a result, the water content in the slurry was dehydrated to 20%, and clear, odorless water with no turbidity was obtained as the filtrate. was taken. The filtrate was directly returned to the pond.

Further, this slurry was dried in the sun for 7 days, and as a result, the water content was dehydrated to 12% and a solid dried sludge was obtained.
(Example 22)

By dissolving the natural hot spring concentrate of the present invention in water at a concentration of 50 to 100 ppm and applying this aqueous solution by soaking the seeds of agricultural crops and spraying them on the foliage, it is possible to promote germination and rooting, and to improve the growth of leaves, stems, and branches. It showed excellent results in elongation, ripening of fruit, increase in sugar content, strengthening of tree vigor, etc. In particular, in hydroponics, it showed excellent results compared to the conventional method. In addition, since the natural hot spring concentrate of the present invention contains various minerals in a well-balanced and well-absorbed state, it has the effect of protecting crops from various agricultural diseases caused by mineral deficiency. I understand.

In addition, (1) hot spring concentrated undiluted solution is produced by adding a large amount of active minerals of hot springs, (2) natural minerals are added to natural hot springs to produce high-quality drinking mineral water, and (3) table use. (4) Cleaning solution using bactericidal action, (5) Cosmetics containing natural minerals, (6) Oxidizing organic and inorganic substances by generating OH radicals. (7) It has the effect of reducing clusters of water. (8) It has deodorizing and deodorizing action. (9) It has the effect of culturing and accelerating the growth of microorganisms and plants. (10) It has an action of flocculating suspended solids.
(Example 23)

　The natural hot spring concentrated deactivator of the present invention was dissolved in water at concentrations of 200 and 400 ppm to evaluate virus inactivation. In the inactivation evaluation, a sample is mixed with a virus solution, the virus solution is sampled at each reaction time, cells are infected, and the amount of infectious virus is measured.

The control was a virus solution with a reaction time of 0 minutes, and the reference was tap water (Kiryu City, Gunma Prefecture). The titer (stock solution) is 3.6 x ¹⁰⁴ _TCID50 /mL. The cells used are Vero cells (strain number is JCRB0111, and the medium composition is 10% serum (FBS) DMEM.

The test method is as follows. First, prepare the virus. After adjusting the Vero cells to 3.0 x 105 cells/mL, pour 10 mL into a 75 cm ² tissue culture and incubate for 1 day at 37°C in a 5% CO2 incubator. 2 was seeded at 100 uL. Then, after culturing for 3 days in an incubator with a CO2 concentration of 5%, the virus titer was measured based on the TCID50 method.

Next, we prepared the cells. After adjusting the Vero cells to 1.0×10 5 cells/mL, 100 μL/well was seeded in a 96-well plate and cultured for 1 day at 37° C. in a 5% CO 2 incubator.

Next, I prepared the subject. 975 uL of each specimen was seeded in a 24-well plate.

In addition, we confirmed the reaction between the virus and the sample. 25 μL of the virus liquid was mixed with the subject and allowed to react. The reaction time was 1 minute. After that, 100 uL of the virus mixture was added to 900 uL of serum-free DMEM medium to stop the reaction. The reaction between the virus and the sample was confirmed in a cabinet at room temperature of 23°C and humidity of 44%.

In addition, it was added to Vero cells and cultured. 100 uL/well of the solution diluted in the sample preparation was seeded on the cells prepared in the virus preparation step, 3 wells per sample per reaction time. After culturing for 1 day in an incubator at 37°C and 5% CO2 concentration, the amount of infectious virus was measured to evaluate the antiviral activity of each subject.

Based on the above, the results are as follows, with the viral infectious titer of each sample as 100 for the control.

The 400 ppm natural hot spring concentrate is shown in Fig. 11 and Table 4, and it was confirmed that the virus decreased to about 8% after 1 minute with respect to the virus infectivity titer at 0 minutes of reaction time.

With 200 ppm of the natural hot spring concentrate, it was confirmed that the virus decreased to about 22% after 1 minute with respect to the virus infectivity titer at the reaction time of 0 minutes. FIG. 11 shows the virus infectivity titer of tap water, 400 ppm natural hot spring concentrate, and 200 ppm natural hot spring concentrate when the virus infectivity titer of the control is set to 100. Numerical values are shown in Table 4.

Next, Table 5 and Fig. 12 show the virus reduction rate for each sample of tap water, natural hot spring concentrate 400 ppm, and natural hot spring concentrate 200 ppm relative to the control.

With 400 ppm natural hot spring concentrate, a 91.9% decrease was confirmed after 1 minute compared to the virus infectivity titer at 0 minute reaction time.

A natural hot spring concentrate of 200 ppm decreased the virus infectivity by 77.6% after 1 minute compared to the virus infectivity titer at 0 minute reaction time.

In addition, Table 6 and Figure 13 show the inactivation rate for the reference.

　The 400ppm natural hot spring concentrate confirmed 90.1% inactivation compared to the reference (tap water) from the virus reduction rate after 1 minute.

200 ppm of natural hot spring concentrate was confirmed to inactivate 72.5% of the reference (tap water) from the virus reduction rate after 1 minute.

From this result, it was confirmed that the virus infection titer was reduced to 1/10 or less after 1 minute with 400 ppm natural hot spring concentrate. In addition, since the virus reduction in the reference was also confirmed, it is appropriate to use the value of the virus inactivation rate against the reference (Fig. 13, Table 6) as an evaluation index of the inactivation ability of the natural hot spring concentrate. be judged. In other words, the inactivation ability of 400ppm natural hot spring concentrate is 90.1% in 1 minute.

In carrying out this example, the following cytotoxicity evaluation (MTT assay) was performed in order to confirm that the test subject did not show toxicity to cells in this example.

In the outline of the cytotoxicity evaluation, the "virus-specimen reaction" test was tested with 25 uL of serum-free EM instead of the virus solution, a diluted solution of the solution that contacted the subject was prepared, and cytotoxicity was evaluated.

This test optically measures the enzymatic activity that reduces the MTT dye that occurs in cells to a formazan dye. As a result, when the number of cells decreases due to death or the like, the amount of luminescence decreases.

First, to prepare the cells, Vero cells were adjusted to 1.0 x 105 cells/mL, seeded in 100uL/well on a 96-well plate, and cultured for 1 day at 37°C in a 5% CO2 incubator.

Subsequently, 975 uL of each test sample was seeded in a 24-well plate to prepare the test sample.

Furthermore, as a reaction between serum-free DMEM and the specimen, 25 µL of serum-free DMEM was mixed with the specimen and allowed to react. The reaction time was 1 minute. Then, 100 uL of the serum-free DMEM mixture was added to 900 uL of serum-free DMEM medium to stop the reaction. The reaction between serum-free DMEM and the sample was performed in a cabinet at room temperature of 23°C and humidity of 20%.

Next, for addition and culture of Vero cells, 10-fold and 100-fold diluted solutions were added to the cells prepared in Cell preparation at 100 µL/well, 3 wells per sample. sown. After that, it was cultured for 1 day in an incubator at 37°C and a CO2 concentration of 5%.

Then, as a cytotoxicity test, 10 uL of MTT solution was added to each well and allowed to undergo color reaction for 2 hours in an incubator at 37°C and 5% CO2 concentration. After that, 100 uL of the solubilizing solution was added, and the absorbance at 595 nm was measured using a microplate reader.

（実施例２４） In addition, as a result of the cytotoxicity test, the absorbance value of the cells exposed to the serum-free DMEM medium that was not in contact with the subject (Fig. 14, Table 7), the Kraft hot spring 400 ppm and the Kraft hot spring 200 ppm. No decrease was observed, confirming that the solution in contact with each subject was not toxic to the cells.

(Example 24)

An antibacterial test was conducted by dissolving the natural hot spring concentrate of the present invention in water. In the antibacterial test, towels were washed with natural hot spring concentrate, and the antibacterial activity value was measured before and after washing. Towels were washed according to the method for washing SEK mark textile products and the standard washing method established by the Textile Evaluation Technology Council. In the washing, a test solution was used, which was obtained by adding a test bacteria solution containing 0.05% of a surfactant (Tweena80) to a natural hot spring concentrate. The test was performed on the surface of a handkerchief.

In the antibacterial test, bacteria obtained from the surface of towels before washing (0 times of washing) and 10 times of washing were inoculated on the entire surface of the agar medium, and samples were placed on the surface and cultured. The antibacterial activity was qualitatively examined from the presence or absence of a growth inhibition zone (halo) around the cultured sample. This antibacterial test used Staphylococcus aureus using the JIS-L-1902 bacterial liquid absorption method. Either can be used to have a growth inhibitor. All of the measurement results revealed that growth inhibitors grew and had sufficient antibacterial properties (Table 8).

action

The mechanism of action of the natural hot spring concentrate of the present invention is still unclear, but it is speculated as follows. That is, when the mineral liquid (trade name: Kraft Onsen (registered trademark)) extracted with mineral acid from the hot spring shale layer, which is the raw material for the natural hot spring concentrate of the present invention, is added to wastewater, gas is generated and two-phase separation occurs. Decreases COD. Analysis revealed that the gas produced was CO2. It is considered that this is because the organic matter in the wastewater was oxidized by the Kraft Hot Springs (registered trademark). From the ESR measurement results, it was measured that the addition of Kraft Hot Springs (registered trademark) generated OH radicals with strong oxidizing power.

In addition, biotite, which is one of the preferred starting materials for natural hot spring concentrates, is a compound belonging to phyllosilicates and has a layered structure. Various ions, including these ions, are eluted in the form of multiple ions, complex ions, or water-soluble oxides, in addition to simple ions, by pretreatment, if necessary, followed by mineral acid treatment. Conceivable. The reason why biotite is particularly good among phyllosilicates is that biotite itself contains a wide variety of minerals compared to other minerals, and in the case of biotite, it is easy to extract these minerals due to its structure. is. The same can be said for vermiculite, which is a weathered body of granite.

These minerals contained in the obtained natural hot spring concentrate are the same as those that are gradually eluted naturally, so they are essential elements for living organisms such as animals and plants, and are presumed to promote their growth. be.

Claims (16)

A metal-containing acidic solution containing natural hot spring ingredients and inorganic acid-treated minerals containing minerals, characterized by containing organic and inorganic substances in the hot spring liquid and having the property of oxidizing them. Natural hot spring concentrate for inorganic oxidation treatment. It contains 1 to 20 g/liter of iron, 1 to 10 g/liter of magnesium and 1 to 10 g/liter of potassium as main components, and has a specific gravity of 1.1 to 1.5 and a pH of 0.1 to 2.0. The natural hot spring concentrate according to claim 1. As metal components, it contains large amounts of iron, magnesium and potassium, and contains most of the metals consisting of phosphorus, titanium, calcium, manganese, sodium, silica, cobalt, lithium, copper, nickel, zinc and barium, and arsenic The natural hot spring concentrate according to claim 1, which does not contain , lead, mercury and chromium. A metal-containing acidic solution containing natural hot spring ingredients and inorganic acid-treated minerals containing minerals, characterized by containing organic and inorganic substances in the hot spring liquid and having the property of oxidizing them. A virus inactivating agent consisting of a natural hot spring concentrate for inorganic substance oxidation treatment. It contains 1 to 20 g/liter of iron, 1 to 10 g/liter of magnesium and 1 to 10 g/liter of potassium as main components, and has a specific gravity of 1.1 to 1.5 and a pH of 0.1 to 2.0. The virus inactivating agent according to claim 4, wherein As metal components, it contains large amounts of iron, magnesium and potassium, and contains most of the metals consisting of phosphorus, titanium, calcium, manganese, sodium, silica, cobalt, lithium, copper, nickel, zinc and barium, and arsenic 5. The virus inactivating agent according to claim 4, which does not contain , lead, mercury or chromium. A metal-containing acidic solution containing natural hot spring ingredients and inorganic acid-treated minerals containing minerals, characterized by containing organic and inorganic substances in the hot spring liquid and having the property of oxidizing them. Liquid fertilizer made from natural hot spring concentrate for inorganic substance oxidation treatment. It contains 1 to 20 g/liter of iron, 1 to 10 g/liter of magnesium and 1 to 10 g/liter of potassium as main components, and has a specific gravity of 1.1 to 1.5 and a pH of 0.1 to 2.0. The liquid fertilizer according to claim 7. - As metal components, it contains large amounts of iron, magnesium and potassium, and contains most of the metals consisting of phosphorus, titanium, calcium, manganese, sodium, silica, cobalt, lithium, copper, nickel, zinc and barium, and arsenic 8. The liquid fertilizer according to claim 7, which does not contain lead, mercury or chromium. A metal-containing acidic solution containing natural hot spring ingredients and inorganic acid-treated minerals containing minerals, characterized by containing organic and inorganic substances in the hot spring liquid and having the property of oxidizing them. Natural hot spring concentrated drinking water for inorganic oxidation treatment. It contains 1 to 20 g/liter of iron, 1 to 10 g/liter of magnesium and 1 to 10 g/liter of potassium as main components, and has a specific gravity of 1.1 to 1.5 and a pH of 0.1 to 2.0. The natural hot spring concentrated drinking water according to claim 10. As metal components, it contains large amounts of iron, magnesium and potassium, and contains most of the metals consisting of phosphorus, titanium, calcium, manganese, sodium, silica, cobalt, lithium, copper, nickel, zinc and barium, and arsenic 11. The natural hot spring concentrated drinking water according to claim 10, which does not contain , lead, mercury and chromium. A metal-containing acidic solution containing natural hot spring ingredients and inorganic acid-treated minerals containing minerals, characterized by containing organic and inorganic substances in the hot spring liquid and having the property of oxidizing them. Natural hot spring concentrate for inorganic oxidation treatment. It contains 1 to 20 g/liter of iron, 1 to 10 g/liter of magnesium and 1 to 10 g/liter of potassium as main components, and has a specific gravity of 1.1 to 1.5 and a pH of 0.1 to 2.0. 14. The natural hot spring concentrate according to claim 13. As metal components, it contains large amounts of iron, magnesium and potassium, and contains most of the metals consisting of phosphorus, titanium, calcium, manganese, sodium, silica, cobalt, lithium, copper, nickel, zinc and barium, and arsenic 14. The natural hot spring concentrate according to claim 13, which does not contain , lead, mercury and chromium. A method for producing a metal-containing acidic solution containing natural hot spring ingredients and inorganic acid-treated minerals containing minerals, characterized by containing organic and inorganic substances in the hot spring liquid and having the property of oxidizing them. A method for producing a natural hot spring concentrate for oxidizing organic matter and inorganic matter in water.

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