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WO2023187038A1 - Procédé et agencement de mesure pour examiner un matériau organique - Google Patents

Procédé et agencement de mesure pour examiner un matériau organique Download PDF

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Publication number
WO2023187038A1
WO2023187038A1 PCT/EP2023/058262 EP2023058262W WO2023187038A1 WO 2023187038 A1 WO2023187038 A1 WO 2023187038A1 EP 2023058262 W EP2023058262 W EP 2023058262W WO 2023187038 A1 WO2023187038 A1 WO 2023187038A1
Authority
WO
WIPO (PCT)
Prior art keywords
radiation
measuring arrangement
arrangement according
organic material
excitation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2023/058262
Other languages
German (de)
English (en)
Inventor
Mathias Belz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lytegate GmbH
Original Assignee
Lytegate GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lytegate GmbH filed Critical Lytegate GmbH
Priority to DE112023001801.4T priority Critical patent/DE112023001801A5/de
Publication of WO2023187038A1 publication Critical patent/WO2023187038A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
    • G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N2021/4704—Angular selective
    • G01N2021/4711—Multiangle measurement

Definitions

  • the invention relates to a method for examining, in particular for determining, the vitality of organic material and a measuring arrangement for carrying out the method according to the invention.
  • NADH nicotinamide adenine dinucleotide
  • NADH has the property of showing fluorescence in the range of 450nm when irradiated with electromagnetic radiation with a wavelength of approximately 340nm.
  • the appearance of fluorescence alone is not sufficient to make quantitative statements about the condition of organic material, especially living cells.
  • the object of the present invention is to provide a method and a measuring arrangement which allows an improved determination of the condition of living cells.
  • the organic material is irradiated with excitation radiation, for example in the wavelength range of 340 nm, and the intensity of the fluorescent radiation excited by the irradiation is determined.
  • the organic material is additionally irradiated with electromagnetic scattered radiation, in which scattering occurs on the material and the scattered radiation is subsequently recorded.
  • Scattered radiation in the sense of the present application is understood to mean radiation irradiated onto the material and not the scattered radiation mentioned above.
  • the scattering measurement makes it possible to make statements about the concentration of organic material in the sample volume, so that the measured fluorescence radiation can be better interpreted based on this additional information.
  • the scattered radiation and/or the fluorescent radiation can be recorded at at least two different angles relative to an irradiation direction, with a first angle being between 160° and 200°, in particular 180°.
  • a second angle can be between 70° and 110°, in particular 90°; a third angle can be between 0° and 40°, in particular 20°.
  • This angular distribution makes it possible to make statements about vitality, for example, over a wide range of turbidity levels and thus concentrations of organic material.
  • the organic material can be irradiated alternately with excitation radiation and scattered radiation, so that a simple association of excited fluorescence and scattering is possible.
  • the scattered radiation has a wavelength in the range of 450 nm
  • a single type of detector can advantageously be used.
  • an apparatus can also be set up in which the radiation source and detectors are swapped so that one detector and multiple Radiation sources from different angles generate the same excitation/emission geometry.
  • the excitation radiation can be in the range of 340nm to determine the vitality of cells. If the organic material is irradiated with excitation radiation in the wavelength range of 260-280nm, the protein content in the sample volume can also be determined using another fluorescence measurement.
  • the organic material can be irradiated with scattered radiation in the wavelength range of 650nm or 850nm.
  • the intensities of the scattered radiation and the fluorescent radiation can advantageously be put into a mathematical relationship with one another; For example, a quotient can be formed.
  • a complex analysis models such as an adapted multivariate analysis or algorithms with artificial intelligence, to link the measurements in such a way that the vitality variable can be determined in a self-learning manner
  • the meaningfulness of the measurement can be further improved.
  • a measuring arrangement according to the invention for the optical examination of organic material includes
  • At least one first electromagnetic radiation source for irradiating the material in an irradiation direction with excitation radiation, in particular with a wavelength of 340nm or 260-280nm
  • At least one excitation detector which is set up to detect fluorescent radiation excited by the excitation radiation, in particular only to detect electromagnetic radiation in a wavelength range of 430nm to 470nm, preferably in a wavelength range of 450nm, or in a wavelength range of 280nm-350nm - at least one second radiation source, which is set up to additionally irradiate the organic material with electromagnetic scattered radiation
  • the measuring arrangement can be implemented in a simple manner in that the detector for detecting the scattered radiation is the excitation detector.
  • the second radiation source is suitable for emitting electromagnetic radiation in a wavelength range of 450nm or 650nm or 850nm.
  • the sample volume can be arranged in a traditionally manufactured and cleanable flow cell made of classic materials (stainless steel, PEEK, Teflon, sapphire, etc.) or in the form of a single-use flow cell with a supply line and a discharge line for the material to be examined ;
  • classic materials stainless steel, PEEK, Teflon, sapphire, etc.
  • a further radiation source is present which is designed to emit only electromagnetic radiation with a wavelength of 260nm-280nm; There is also at least one detector that is set up to only detect electromagnetic radiation in a wavelength range of 280nm-350nm.
  • the transmission direction of a radiation source and the reception direction of a corresponding detector can in particular include an angle of 170°-200°, in particular 180°.
  • the transmission direction of a radiation source and the reception direction of a corresponding detector can include an angle of 70°-110°, in particular 90°. Additionally or alternatively, the transmission direction of a radiation source and the reception direction of a corresponding detector can include an angle of 0°-40°, in particular 20°.
  • a further radiation source which is set up to emit only electromagnetic radiation with a wavelength of, for example, 650nm or 850nm and at least one detector which is set up to only emit electromagnetic radiation in a wavelength range of 650nm or 850nm to detect. Any fluorescence artifacts can be avoided, particularly when using scattered radiation in the range of 850 nm.
  • a deflection element which is designed to direct at least part of the electromagnetic radiation emitted by the radiation source directly onto a detector, a reference measurement can be carried out.
  • the deflection element on the light source or sources can in particular be a beam splitter.
  • the beam splitter can, for example, comprise a quartz glass film tilted relative to the direction of incidence of the radiation;
  • the quartz glass film can be arranged tilted by approximately 45° relative to the direction of incidence of the radiation.
  • an optical element for example a dichroic mirror, can be present, which is designed to direct the excitation radiation and the scattered radiation at least in sections towards the sample volume along the same path to steer.
  • Figure 1 shows a possible embodiment of a measuring arrangement according to the invention.
  • Figure 1 shows a schematic representation of a first possible embodiment of a measuring arrangement according to the invention. Shown is a sample volume 1, which contains, for example, a nutrient solution to be examined with organic Material, in particular a number of cells. It goes without saying that in a real application the sample volume 1 can be arranged, for example, in a flow measuring cell, an immersion probe or a cuvette.
  • a sample volume 1 contains, for example, a nutrient solution to be examined with organic Material, in particular a number of cells. It goes without saying that in a real application the sample volume 1 can be arranged, for example, in a flow measuring cell, an immersion probe or a cuvette.
  • the vitality of the cells in the sample volume is determined by emitting excitation radiation 3 in the direction of the sample volume 1 by means of an excitation radiation source 2, which can be designed, for example, as an LED.
  • the direction of irradiation is indicated by an arrow in the figure.
  • the excitation radiation 3 can in particular be in a wavelength range of approximately 340nm -360nm or also in the range of approximately 260-280nm.
  • the excitation radiation 3 passes through an optical element designed as a dichroic mirror 4, which is designed in such a way that it largely transmits the excitation radiation 3.
  • the excitation radiation 3 passes through a deflection element designed as a beam splitter 5, in which it is divided into a reference component 6 and a measurement component 7.
  • the measuring portion 7 subsequently reaches the sample volume 1, where it interacts with the nutrient solution and the biological material contained therein.
  • the incident excitation radiation causes fluorescence at approx.
  • excitation radiation in the range of 470 nm can be used; the corresponding fluorescence radiation is in the range of 420nm.
  • a change statement can be generated about the quotient of the two signals, in which the quotient is invariant compared to the absolute intensities.
  • the detectors 8, 9, 10 are arranged at angles of 180°, 90° and 20° to the incident excitation radiation 3 and with filters 11, 12, 13 provided, which only show transmission for a wavelength of 450 nm, so that only the excited fluorescence radiation 14 can pass through and reach the detectors 8,9,10.
  • the filters can of course be adapted accordingly with regard to their transmission properties for other variants of the measurement or can be designed to be switchable.
  • a scattered light measurement in order to provide the radiation used for this purpose, hereinafter referred to as scattered radiation, a further radiation source 15 is present, which emits electromagnetic radiation at a wavelength in the range of 450 nm. After emerging from the radiation source 15, this scattered radiation 16 first hits the dichroic mirror 4, where it is reflected in the direction of the sample volume 1 due to the transmission properties of the dichroic mirror 4. It is advantageous if the scattered radiation 16 is reflected in that area of the dichroic mirror 4 in which the excitation radiation 3 also passes through the mirror 4, since in this case largely identical conditions are created for the geometric beam path of the scattered radiation 16 and the excitation radiation 3 .
  • the scattered radiation 16 also passes through the beam splitter 5, where a reference component 17 is also branched off from the scattered radiation 16.
  • the reference components 6 and 17 of the excitation and scattered radiation 3 and 16 are recorded on a reference detector 18 and are used, for example, to detect intensity fluctuations in the radiation emitted by the radiation sources 2 or 15 and to take them into account when evaluating the measurements.
  • the measurement portion of the scattered radiation 16 subsequently enters the sample volume 1, where scattering occurs at the cells present in the sample volume 1 as scattering centers, so that the radiation 16 that passes through unscattered and the scattered one Radiation 19 can be recorded by the already mentioned detectors 8,9,10 at different angular ranges.
  • the extent to which scattering occurs will depend on the degree of turbidity of the nutrient solution, and in particular on the concentration of the cells present in the nutrient solution. If the concentration of cells in the nutrient solution is low, it can be assumed that a significant proportion of the scattered radiation 16 passes through the nutrient solution unscattered, i.e. reaches the detector 8 at 180°. As the concentration of cells in the nutrient solution increases, the intensity of the scattered radiation 19 will increase in the detectors 9 and 10 arranged at 90° and 20°, respectively.
  • the sample volume 1 is advantageously irradiated either with excitation radiation 3 or with scattered radiation 16.
  • the vitality of the cells present in the nutrient solution is determined by recording the excited fluorescence.
  • the significance of this measurement is increased in that the concentration of the cells in the nutrient solution is determined by means of the scatter measurement.
  • a high measured intensity of the fluorescence radiation 14 at a low concentration of cells in the nutrient solution is an indicator of a high proportion of vital cells.
  • a low intensity of the fluorescent radiation 14 is an indicator of low cell vitality.
  • the invention makes it possible to simultaneously analyze dissolved substances and particles in a liquid. Particle scattering, fluorescence or absorption can be detected forwards, sideways or backwards.
  • the measuring range of the scattered light measurement of particles can be expanded in particular by measuring depending on the angle, for example first forwards, then sideways and finally, when intensity can no longer be measured in the forward direction, backwards.
  • the combined and angle-dependent measurement of scattered light and fluorescence can be used to - to simultaneously measure scattered light and fluorescence of particles
  • the described concept of angle-dependent measurement and thus extension of the measuring range can be used for all spectroscopic methods, such as Raman, luminescence, etc., especially in those cases where the light to be detected has a Lambertian character, i.e. radiates uniformly in all directions .
  • the radiation excited by the effects described above, i.e. in particular Raman radiation and luminescence radiation would replace or be added to the fluorescent radiation described above.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Engineering & Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne un procédé pour examiner un matériau organique, le matériau organique étant irradié par un rayonnement d'excitation (3), et l'intensité du rayonnement fluorescent (14) excité par l'irradiation étant déterminée. Le matériau organique est en outre irradié par un rayonnement diffusé électromagnétique (16), la diffusion se produisant sur le matériau et le rayonnement diffusé (19) étant absorbé. L'invention concerne en outre un dispositif de mesure correspondant.
PCT/EP2023/058262 2022-03-31 2023-03-30 Procédé et agencement de mesure pour examiner un matériau organique Ceased WO2023187038A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE112023001801.4T DE112023001801A5 (de) 2022-03-31 2023-03-30 Verfahren und Messanordnung zur Untersuchung organischen Materials

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102022107746.2 2022-03-31
DE102022107746.2A DE102022107746A1 (de) 2022-03-31 2022-03-31 Verfahren und Messanordnung zur Untersuchung organischen Materials

Publications (1)

Publication Number Publication Date
WO2023187038A1 true WO2023187038A1 (fr) 2023-10-05

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Application Number Title Priority Date Filing Date
PCT/EP2023/058262 Ceased WO2023187038A1 (fr) 2022-03-31 2023-03-30 Procédé et agencement de mesure pour examiner un matériau organique

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DE (2) DE102022107746A1 (fr)
WO (1) WO2023187038A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5895922A (en) * 1996-03-19 1999-04-20 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence Fluorescent biological particle detection system
US6532067B1 (en) * 1999-08-09 2003-03-11 The United States Of America As Represented By The Secretary Of The Army Aerosol fluorescence spectrum analyzer for rapid measurement of single airborne particles
US20060197033A1 (en) * 2005-02-08 2006-09-07 Northrop Grumman Corporation Systems and methods for use in detecting harmful aerosol particles
US9423350B2 (en) * 2013-02-15 2016-08-23 Vwm Gmbh Method and device for determining a concentration

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4650336A (en) 1985-09-20 1987-03-17 Advanced Genetic Sciences, Inc. Measurement of variable fluorescence of plants
NL1002870C2 (nl) 1996-04-15 1997-10-17 Inst Voor Agrotech Onderzoek Werkwijze en stelsel voor het bepalen van de kwaliteit van een gewas.
DE19845883B4 (de) 1997-10-15 2007-06-06 LemnaTec GmbH Labor für elektronische und maschinelle Naturanalytik Verfahren zur Bestimmung der Phytotoxizität einer Testsubstanz
US7116354B2 (en) 2001-06-20 2006-10-03 Xenogen Corporation Absolute intensity determination for a light source in low level light imaging systems
NL1021476C2 (nl) 2002-09-17 2004-03-18 Plant Res Int Bv Werkwijze en inrichting voor het bepalen van de kwaliteit van plantaardig materiaal en werkwijze en inrichting voor het sorteren van plantaardig materiaal.
WO2009043050A2 (fr) 2007-09-27 2009-04-02 Duke University Système de bioessais optiques à réseau d'imagerie à sondes multiples
US9820655B2 (en) 2007-09-28 2017-11-21 Duke University Systems and methods for spectral analysis of a tissue mass using an instrument, an optical probe, and a Monte Carlo or a diffusion algorithm
EP2056094A1 (fr) 2007-10-12 2009-05-06 Universiteit Hasselt Système d'imagerie par fluorescence
GB0808340D0 (en) 2008-05-08 2008-06-18 Univ Edinburgh Remote sensing system
DE102010047237B4 (de) 2010-08-13 2021-07-01 Leica Microsystems Cms Gmbh Verfahren zum Trennen von Detektionssignalen im Strahlengang einer optischen Einrichtung
DE102011100507B4 (de) 2011-04-29 2020-05-14 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Tragbares optisches Analysegerät
DE102011101934B4 (de) 2011-05-18 2017-07-06 Christian-Albrechts-Universität Zu Kiel Großflächiger Biofilmsensor
DE102011118619A1 (de) 2011-11-16 2013-05-16 Forschungszentrum Jülich GmbH Vorrichtung und Verfahren zur Erfassung von Wachstumsprozessen und simultanen Messung von chemisch-physikalischen Parametern
EP2856118B1 (fr) 2012-05-30 2017-10-25 Board Of Trustees Of Michigan State University Systèmes de phénométrie végétale et procédé
EP2710883A1 (fr) 2012-09-24 2014-03-26 Heliospectra AB Optimisation de spectre pour éclairage artificiel
EP2887053A1 (fr) 2013-12-18 2015-06-24 Basf Se Détermination d'une infection fongique d'une plante par fluorescence de chlorophylle induite par longueurs d'onde d'excitation différentes
DE102014212657B4 (de) 2014-06-30 2016-03-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. System und Verfahren zur bedarfsgerechten Zuführung von Beleuchtungsenergie an Pflanzen
DE102014218202A1 (de) 2014-09-11 2016-03-17 Iwasaki Electric Co., Ltd. Abbildungssystem
GB2545843B (en) 2014-10-16 2020-10-07 Hitachi High Tech Corp Fixed position controller and method
AU2017370681A1 (en) 2016-12-06 2019-06-27 Pendulum Therapeutics, Inc. Methods and compositions relating to isolated and purified microbes
DE102017001588B4 (de) 2017-02-17 2018-09-06 aquila biolabs GmbH Verfahren und Vorrichtung zur Abstimmung optischer Messungen an kontinuierlich durchmischten Reaktoren
US10852236B2 (en) 2017-09-12 2020-12-01 Curadel, LLC Method of measuring plant nutrient transport using near-infrared imaging
DE102019208833A1 (de) 2019-06-18 2020-12-24 Robert Bosch Gmbh Verfahren zum Kennzeichnen von Pflanzenbildinformationen, insbesondere für landwirtschaftliche Zwecke
DE102019131650A1 (de) 2019-11-22 2021-05-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zum Ermitteln und Optimieren des Gehalts von wenigstens einem Pflanzeninhaltsstoff von wenigstens einem Teil einer Pflanze
CN212780522U (zh) 2020-07-16 2021-03-23 南京大学 一种便携式溶解性有机物与浊度水质分析仪

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5895922A (en) * 1996-03-19 1999-04-20 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence Fluorescent biological particle detection system
US6532067B1 (en) * 1999-08-09 2003-03-11 The United States Of America As Represented By The Secretary Of The Army Aerosol fluorescence spectrum analyzer for rapid measurement of single airborne particles
US20060197033A1 (en) * 2005-02-08 2006-09-07 Northrop Grumman Corporation Systems and methods for use in detecting harmful aerosol particles
US9423350B2 (en) * 2013-02-15 2016-08-23 Vwm Gmbh Method and device for determining a concentration

Also Published As

Publication number Publication date
DE112023001801A5 (de) 2025-02-20
DE102022107746A1 (de) 2023-10-05

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