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WO2023001839A3 - A detection kit and methods of detection of infectious agents by fluorescent aptamer-magnetic bead sandwich assay - Google Patents

A detection kit and methods of detection of infectious agents by fluorescent aptamer-magnetic bead sandwich assay Download PDF

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Publication number
WO2023001839A3
WO2023001839A3 PCT/EP2022/070244 EP2022070244W WO2023001839A3 WO 2023001839 A3 WO2023001839 A3 WO 2023001839A3 EP 2022070244 W EP2022070244 W EP 2022070244W WO 2023001839 A3 WO2023001839 A3 WO 2023001839A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
infectious agent
kit
methods
magnetic bead
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2022/070244
Other languages
French (fr)
Other versions
WO2023001839A2 (en
Inventor
Anna GUILDFORD
Sophia KHAN
Flavia BONALUMI
Michela BUONOCORE
Anna Maria D'URSI
Matteo Santin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tissue Click Ltd
Original Assignee
Tissue Click Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tissue Click Ltd filed Critical Tissue Click Ltd
Publication of WO2023001839A2 publication Critical patent/WO2023001839A2/en
Publication of WO2023001839A3 publication Critical patent/WO2023001839A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The present invention relates to a kit for the antibody-free concentration and detection of specific biomarkers of viral and microbial infection diseases. More specifically, the kit relies on the capture of the infectious agent or its compounds by superparamagnetic nanoparticles functionalised with biomarker-specific peptide aptamers. Magnets enable the concentration of the infectious agent or its fragments in a biological test sample thus enhancing the sensitivity of the detection. The detection is then achieved by the recognition of separate domains of the same or different biomarkers of the infectious agent by different peptide aptamers conjugated with one or more detectable labels, for example fluorophore molecules. The presence of the infectious agent and/or of its fragments may be rapidly detected by the naked eye using a portable illumination apparatus equipped with a fluorescent torch.
PCT/EP2022/070244 2021-07-19 2022-07-19 A detection kit and methods of detection of infectious agents Ceased WO2023001839A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB2110391.6 2021-07-19
GBGB2110391.6A GB202110391D0 (en) 2021-07-19 2021-07-19 A detection kit and methods of detection of infectious agents

Publications (2)

Publication Number Publication Date
WO2023001839A2 WO2023001839A2 (en) 2023-01-26
WO2023001839A3 true WO2023001839A3 (en) 2023-03-16

Family

ID=77443526

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2022/070244 Ceased WO2023001839A2 (en) 2021-07-19 2022-07-19 A detection kit and methods of detection of infectious agents

Country Status (2)

Country Link
GB (1) GB202110391D0 (en)
WO (1) WO2023001839A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
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WO2005004798A2 (en) * 2003-06-10 2005-01-20 The J. David Gladstone Institutes Methods for treating lentivirus infections

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US20090291508A1 (en) 2008-05-20 2009-11-26 Rapid Pathogen Screening Inc. Nanoparticles in diagnostic tests
WO2007117444A2 (en) 2006-03-31 2007-10-18 Yinghe Hu Protein detection by aptamers
US20130137090A1 (en) 2010-07-06 2013-05-30 Aptateck Bio Ltd. Nucleic acid aptamer-based diagnostic methods with novel techniques for signal enhancement
US9891227B2 (en) 2014-05-02 2018-02-13 Academia Sinica Ultrasensitive detection of a biological target by aptamer-conjugated gold nanoparticles
US10308989B2 (en) 2014-06-13 2019-06-04 North Carolina State University Aptamers with binding affinity to norovirus
KR102064961B1 (en) 2017-12-13 2020-01-10 주식회사 포스코 Magnetic nanoparticles and method for amplification of signal in lateral flow assay by using the same
WO2020055348A2 (en) 2018-03-01 2020-03-19 Sentuerk Erol Production system of a cast aluminum saucepan with an induction base
US20220307080A1 (en) 2019-06-07 2022-09-29 Simon Fraser University Methods and reagents for nucleic acid amplification and/or detection
WO2021007249A1 (en) 2019-07-08 2021-01-14 Armitstead Annie Pitts Paper lateral flow immunoassay
BR102021003012A2 (en) 2020-02-19 2021-11-30 Univ Berlin Charite Methods for diagnosing sars-cov-2 infection, kit and uses
US10948490B1 (en) 2020-04-01 2021-03-16 Institut Pasteur Severe acute respiratory syndrome (SARS)—associated coronavirus diagnostics
US10787501B1 (en) 2020-04-02 2020-09-29 Regeneron Pharmaceuticals, Inc. Anti-SARS-CoV-2-spike glycoprotein antibodies and antigen-binding fragments
US10845336B2 (en) 2020-04-21 2020-11-24 Mohammad Abdolahad Electrochemical approach for COVID-19 detection

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005004798A2 (en) * 2003-06-10 2005-01-20 The J. David Gladstone Institutes Methods for treating lentivirus infections

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
"Biobased Surfactants", 1 January 2019, ELSEVIER, UK, ISBN: 978-0-12-812705-6, article CLENDENNEN STEPHANIE K. ET AL: "Betaine Amphoteric Surfactants-Synthesis, Properties, and Applications", pages: 447 - 469, XP055981655, DOI: 10.1016/B978-0-12-812705-6.00014-9 *
BRUNO JOHN G ET AL: "Development of a Fluorescent Enzyme-Linked DNA Aptamer-Magnetic Bead Sandwich Assay and Portable Fluorometer for Sensitive and RapidListeriaDetection", JOURNAL OF FLUORESCENCE, SPRINGER US, NEW YORK, vol. 25, no. 1, 17 December 2014 (2014-12-17), pages 173 - 183, XP035446001, ISSN: 1053-0509, [retrieved on 20141217], DOI: 10.1007/S10895-014-1495-8 *
BRUNO JOHN G ET AL: "Infectious Diseases and Epidemiology Fluorescent DNA Aptamer-Magnetic Bead Sandwich Assays and Portable Fluorometer for Sensitive and Rapid Foodborne Pathogen Detection and Epidemiology", JOURNAL OF INFECTIOUS DISEASES AND EPIDEMIOLOGY, vol. 2, no. 1, 28 March 2016 (2016-03-28), XP055980899 *
BRUNO JOHN G. ET AL: "Development of a Fluorescent Enzyme-Linked DNA Aptamer-Magnetic Bead Sandwich Assay and Portable Fluorometer for Sensitive and Rapid Leishmania Detection in Sandflies", JOURNAL OF FLUORESCENCE, vol. 24, no. 1, 1 January 2014 (2014-01-01), New York, pages 267 - 277, XP055980903, ISSN: 1053-0509, Retrieved from the Internet <URL:https://link.springer.com/content/pdf/10.1007/s10895-013-1315-6.pdf> DOI: 10.1007/s10895-013-1315-6 *
DEVI ARPITA ET AL: "Designing of peptide aptamer targeting the receptor-binding domain of spike protein of SARS-CoV-2: an in silico study", MOLECULAR DIVERSITY, SPRINGER INTERNATIONAL PUBLISHING, CHAM, vol. 26, no. 1, 2 January 2021 (2021-01-02), pages 157 - 169, XP037705206, ISSN: 1381-1991, [retrieved on 20210102], DOI: 10.1007/S11030-020-10171-6 *
TEMUR ERHAN ET AL: "Attomole Sensitivity of Staphylococcal Enterotoxin B Detection Using an Aptamer-Modified Surface-Enhanced Raman Scattering Probe", ANALYTICAL CHEMISTRY, vol. 84, no. 24, 27 November 2012 (2012-11-27), US, pages 10600 - 10606, XP055981174, ISSN: 0003-2700, DOI: 10.1021/ac301924f *
XU W ET AL: "Anti-peptide aptamers recognize amino acid sequence and bind a protein epitope", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 93, 1 January 1996 (1996-01-01), pages 7475 - 7480, XP002182269, ISSN: 0027-8424, DOI: 10.1073/PNAS.93.15.7475 *

Also Published As

Publication number Publication date
GB202110391D0 (en) 2021-09-01
WO2023001839A2 (en) 2023-01-26

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