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WO2023086938A3 - Type v nucleases - Google Patents

Type v nucleases Download PDF

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Publication number
WO2023086938A3
WO2023086938A3 PCT/US2022/079715 US2022079715W WO2023086938A3 WO 2023086938 A3 WO2023086938 A3 WO 2023086938A3 US 2022079715 W US2022079715 W US 2022079715W WO 2023086938 A3 WO2023086938 A3 WO 2023086938A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleases
rna guides
type
nucleic acids
acids encoding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2022/079715
Other languages
French (fr)
Other versions
WO2023086938A2 (en
Inventor
David A. Scott
David R. Cheng
Winston X. YAN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arbor Biotechnologies Inc
Original Assignee
Arbor Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arbor Biotechnologies Inc filed Critical Arbor Biotechnologies Inc
Publication of WO2023086938A2 publication Critical patent/WO2023086938A2/en
Publication of WO2023086938A3 publication Critical patent/WO2023086938A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to nucleases or nucleic acids encoding the nucleases, RNA guides or nucleic acids encoding the RNA guides, processes for characterizing the nucleases and/or RNA guides, compositions comprising the nucleases and/or RNA guides, and kits and/or methods for preparing and/or using the nucleases and/or RNA guides.
PCT/US2022/079715 2021-11-12 2022-11-11 Type v nucleases Ceased WO2023086938A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163278685P 2021-11-12 2021-11-12
US63/278,685 2021-11-12

Publications (2)

Publication Number Publication Date
WO2023086938A2 WO2023086938A2 (en) 2023-05-19
WO2023086938A3 true WO2023086938A3 (en) 2023-06-15

Family

ID=86336655

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2022/079715 Ceased WO2023086938A2 (en) 2021-11-12 2022-11-11 Type v nucleases

Country Status (1)

Country Link
WO (1) WO2023086938A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20240376451A1 (en) * 2021-01-07 2024-11-14 Inscripta, Inc. Mad nucleases
WO2024163585A1 (en) * 2023-02-01 2024-08-08 Arbor Biotechnologies, Inc. Gene editing systems comprising type v crispr nuclease and engineered guide rna
WO2025051140A1 (en) * 2023-09-04 2025-03-13 中国农业大学 NEW CRISPR-CASσ ENZYME AND SYSTEM

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200087640A1 (en) * 2017-11-01 2020-03-19 The Regents Of The University Of California Casz compositions and methods of use
US20210324356A1 (en) * 2019-03-07 2021-10-21 The Regents Of The University Of California Crispr-cas effector polypeptides and methods of use thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200087640A1 (en) * 2017-11-01 2020-03-19 The Regents Of The University Of California Casz compositions and methods of use
US20210324356A1 (en) * 2019-03-07 2021-10-21 The Regents Of The University Of California Crispr-cas effector polypeptides and methods of use thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE UNIPROTKB ANONYMOUS : "A0A4R0W5F4 · A0A4R0W5F4_BIFLN", XP093072988, retrieved from UNIPROT *

Also Published As

Publication number Publication date
WO2023086938A2 (en) 2023-05-19

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