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WO2023079539A1 - Méthode de diagnostic d'une infection par le virus sars-cov-2 par l'intermédiaire de capacités d'olfaction canines - Google Patents

Méthode de diagnostic d'une infection par le virus sars-cov-2 par l'intermédiaire de capacités d'olfaction canines Download PDF

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Publication number
WO2023079539A1
WO2023079539A1 PCT/IB2022/060746 IB2022060746W WO2023079539A1 WO 2023079539 A1 WO2023079539 A1 WO 2023079539A1 IB 2022060746 W IB2022060746 W IB 2022060746W WO 2023079539 A1 WO2023079539 A1 WO 2023079539A1
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WO
WIPO (PCT)
Prior art keywords
samples
covid
infection
olfaction
canine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2022/060746
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English (en)
Inventor
Dominique Grandjean
Clothilde LECOQ-JULIEN
Riad Sarkis
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MINISTRY OF INTERIOR
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MINISTRY OF INTERIOR
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Publication date
Application filed by MINISTRY OF INTERIOR filed Critical MINISTRY OF INTERIOR
Priority to EP22889561.1A priority Critical patent/EP4430393A4/fr
Publication of WO2023079539A1 publication Critical patent/WO2023079539A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements
    • A61B10/0045—Devices for taking samples of body liquids
    • A61B10/0064—Devices for taking samples of body liquids for taking sweat or sebum samples
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements
    • A61B10/0096—Casings for storing test samples

Definitions

  • the subject matter in general relates to SARS-CoV-2. More particularly, but not exclusively, the subject matter relates to detection of CO VID- 19 infection caused by SARS-CoV-2 by trained canines.
  • COVID-19 which is caused by SARS-CoV-2 is an enveloped single and positive stranded RNA virus.
  • the RNA of the virus codes for non-structural replicase polyprotein and structural proteins which includes spike (S), envelope (E), membrane (M) and nucleocapsid proteins among others.
  • SARS-CoV-2 is highly contagious and has a latent period of up to 24 days. Even in the latent phase, the virus is highly contagious and the virus carriers do not show any obvious symptoms.
  • RT-PCR reverse transcription polymer chain reaction
  • an individual may be infected with SARS-CoV-2 but may not develop any symptoms as compared to symptomatic individual, in such scenario the individual may not be aware of being infected by the SARS-CoV-2. Hence, the individual may be in contact with others instead of isolating themselves increasing the chances to infect others. Even in public places, it is difficult to figure out individuals who are infected by SARS-CoV-2 increasing the risk of the spread of the infection. [0006] In view of the foregoing discussion, there is a need for improved method for detection of Covid 19 infection to reduce the risk of infection.
  • a method to detect covid 19 infection comprises collecting underarms sweat samples from various individuals. The samples are then stored at a cool temperature, which are then introduced into an apparatus configured to accommodate the samples to enable detection of covid 19 positive infection by a canine.
  • the apparatus comprises an olfaction component detachably connected to a holder.
  • the holder is configured to accommodate a container comprising the samples.
  • the apparatus further comprises a handle, wherein a distal end of the handle is detachably connected to the holder and proximal end of the handle is connected to a platform.
  • the canines are trained to sniff the samples provided in the apparatus to detect the specific odour of the samples.
  • FIG. 1 illustrates a method flow chart that enables detection of covid 19 infection, in accordance with an embodiment
  • FIG.2A illustrates a plurality of polymer tubes 202, in accordance with an embodiment
  • FIG.2B illustrates a container 204 configured to accommodate the polymer tube 202, in accordance with an embodiment
  • FIG.3 illustrates an apparatus 300 configured to accommodate the container 204, in accordance with an embodiment
  • FIG.4 illustrates a plurality of apparatus 300 arranged in a room, in accordance with an embodiment. DETAILED DESCRIPTION OF THE INVENTION
  • a method flowchart 100 for detection of covid 19 infection is disclosed.
  • the method disclosed may be employed to train canines or dogs to detect Covid 19 infection.
  • axillary sweat sample may be collected from different individuals.
  • the sweat sample may be collected from a right and left armpit of the individuals.
  • the samples may be collected from the individuals that are tested positive for Covid 19 infection and from the individuals that are tested negative for Covid 19 infection. Further, samples are not collected from covid 19 positive individuals who are receiving a medical treatment for more than 36 hours to avoid possible interferences with long-term medical treatments in the sweat volatile organic compounds (VOCs).
  • VOCs sweat volatile organic compounds
  • a polymer tube 202 may be used to collect the sweat samples from various individuals.
  • the polymer tube 202 may be placed under the armpit of the individual for a specific time, for example but not limited, to sixty seconds.
  • a sterile gauze swab or a sterile gauze filter may be placed under the armpit of the individual to collect the sweat sample from the individual.
  • the polymer tube 202 adsorbs both polar and a polar molecule.
  • the chemical analysis of the collected samples may also be carried out identify sweat biomarkers of Covid-19.
  • the collected samples are stored in a cool place prior to using the samples to train canines for detection of Covid 19 positive infection.
  • the collected samples are covid 19 positive, such samples may be stored and used for almost six months. However, if the collected samples are covid 19 negative then such samples may be stored and used for no more than two weeks.
  • the samples collected at step 102 may be collected from various hospitals.
  • the information of each of the collected samples may be recorded.
  • the information may pertain to hospital name, reference number, date of sampling, type of material used for sampling, sweat collected from left or right armpit of the individual, and the details if the sample collected is from covid 19 positive or negative individual.
  • a sampler collecting the samples that may be covid positive or covid negative needs to be cautious to avoid contamination of the collected samples.
  • the collected samples may be kept in anti- sterile containers, coded the information as discussed in the previous paragraph and placed in a plastic envelope.
  • the polymer tube 202 that comprises sweat samples collected from different individuals may be placed inside a container 204.
  • the container 204 may be a glass container or any container that serves the purpose of the invention.
  • the container 204 comprising the polymer tube 202 may be introduced into an apparatus 300 at step 106.
  • the apparatus 300 comprises an olfaction component 302, a holder 310, a platform 304 and a stand or handle 306.
  • the olfaction component 302 of the apparatus 300 comprises a first portion and a second portion.
  • the olfaction component 302 may be of a cone shaped configuration.
  • the first portion of the olfaction component 302 may define an opening that tapers towards the second portion.
  • the second portion of the olfaction component 302 may be attached to the holder 310, while the first portion may be facing away from the holder 310.
  • the holder 310 may comprise a base, a pair of supporting arms 308 and a cover portion or a cover element (not shown). Each of the supporting arms 308 may extend from the base and may be parallel to each other.
  • the cover portion may be a plate configured to connect to the proximal ends of each of the supporting arms 308.
  • the cover portion may comprise of a first side and a second side.
  • the second portion of the olfaction component 302 may be attached to the first side of the cover portion of the holder 310.
  • the holder 310 may be configured to accommodate the container 204 comprising the polymer tube 202.
  • the polymer tube 202 comprises the sweat samples collected from various individuals.
  • the container 204 may be positioned in such a way that a top surface of a lid of the container 204 may be attached to a second side of the cover portion of the holder 310.
  • the handle 306 may be provided on the platform 304. A proximal end of the handle 306 may be connected to the platform 304, while a distal end of the handle 306 may be detachably connected to the holder 310.
  • the distal end of the handle 306 may be detachably connected to the first side of the cover element.
  • the canine or dog 402 may be trained to detect covid 19 infection.
  • a series of the apparatus 300 may be arranged in a room. Each of the apparatus 300 may be provided with a single sample that may be covid 19 positive or negative covid 19 sample.
  • the canine 402 may be instructed to sniff each of the samples provided in each of the apparatus 300.
  • the canine 402 may be instructed to place their nose closer to the olfaction component 302 to sniff the sample provided in the holder 310.
  • the canine 402 may be first trained to work on a series of apparatus 300 and further instructed to sit in front of the specific apparatus 300 comprising the covid 19 positive sample.
  • the acquisition of the specific odour of CO VID-19 sweat samples required from one to four hours, with a number of positive samples sniffing ranging from four to ten.
  • sweat odour of the individuals infected with covid 19 have a different odour compared to covid 19 negative individuals due to the virus signature. This difference in the odour of the sweat enables the canine to distinguish between the existence of the covid 19 virus signature in positive individuals compared to the covid negative individuals.
  • the trained canine can discriminate easily between the covid positive and covid negative individual in relation to the excretion of specific catabolites induced by the SARS-CoV-2 virus cellular actions or replications in the sweat.
  • a cleaning protocol may be implemented to avoid any residual odors, as some canines tend to detect the odour of the sample on the apparatus even after the removal of the sample from the apparatus.
  • the cleaning session may be carried out with steamed water after each session, and with a mixture of acetone and water after each training session.
  • an online tool may be implemented to record each canine’s development.
  • the data collected may be used to perform a real-time analysis of performance and accuracy for each canine.
  • the trained canines may be used for detection of covid 19 infected individuals in public places.
  • the method of detection of Covid 19 infection by canine olfaction has detection sensitivities greater than real-time polymerase chain reaction (RT-PCR). Further, employing the method of the present invention, the covid 19 infection may be detected instantly and in a non-invasive way in comparison with the well- known polymerase chain reaction techniques. Moreover, the spread of covid 19 infection can be detected quickly and easily by the method disclosed in the present invention without undergoing the RCT PCR testing. The method disclosed in the present invention allows easy detection of covid 19 positive individuals in public places in a quick manner.
  • RT-PCR real-time polymerase chain reaction
  • the example embodiments described herein may be implemented in an operating environment comprising software installed on a computer, in hardware, or in a combination of software and hardware.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Medical Informatics (AREA)
  • Molecular Biology (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne une méthode de détection d'une infection à covid-19. La méthode comprend la collecte d'échantillons de sueur d'aisselles d'individus. Les échantillons sont stockés dans un récipient stérilisé, et sont ensuite soumis à un appareil. L'appareil est conçu pour recevoir les échantillons afin de permettre la détection d'une infection positive à covid-19 par un canidé. L'appareil comprend un composant d'olfaction relié à un support conçu pour recevoir un récipient comprenant les échantillons collectés. Une extrémité proximale d'une poignée est reliée à une plateforme, tandis qu'une extrémité distale de la poignée est reliée de façon détachable au support. Les canidés sont entraînés à renifler les échantillons fournis dans l'appareil pour détecter une infection à covid-19.
PCT/IB2022/060746 2021-11-08 2022-11-08 Méthode de diagnostic d'une infection par le virus sars-cov-2 par l'intermédiaire de capacités d'olfaction canines Ceased WO2023079539A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP22889561.1A EP4430393A4 (fr) 2021-11-08 2022-11-08 Méthode de diagnostic d'une infection par le virus sars-cov-2 par l'intermédiaire de capacités d'olfaction canines

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US202163276676P 2021-11-08 2021-11-08
US63/276,676 2021-11-08
US202263423531P 2022-11-08 2022-11-08
US63/423,531 2022-11-08

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WO2023079539A1 true WO2023079539A1 (fr) 2023-05-11

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180092335A1 (en) * 2016-10-04 2018-04-05 David R. Hall Animal Olfactory Detection of Disease as Control for Health Metrics Collected by Medical Toilet
US20200008395A1 (en) * 2017-01-14 2020-01-09 Neurrx, SPC Method of using human excrement and secretions for early detection of parkinson's disease

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180092335A1 (en) * 2016-10-04 2018-04-05 David R. Hall Animal Olfactory Detection of Disease as Control for Health Metrics Collected by Medical Toilet
US20200008395A1 (en) * 2017-01-14 2020-01-09 Neurrx, SPC Method of using human excrement and secretions for early detection of parkinson's disease

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GRANDJEAN DOMINIQUE, SARKIS RIAD, LECOQ-JULIEN CLOTHILDE, BENARD AYMERIC, ROGER VINCIANE, LEVESQUE ERIC, BERNES-LUCIANI ERIC, MAES: "Can the detection dog alert on COVID-19 positive persons by sniffing axillary sweat samples? A proof-of-concept study", PLOS ONE, vol. 15, no. 12, 10 December 2020 (2020-12-10), pages e0243122, XP093063593, DOI: 10.1371/journal.pone.0243122 *
MAIA RITA DE CÁSSIA CARVALHO, ALVES LEUCIO CÂMARA, SILVA JEINE EMANUELE SANTOS DA, CZYBA FRANÇOIS RÉMI, PEREIRA JORGE ANTONIO, SOI: "Canine Olfactory Detection of SARS-COV2-Infected Patients: A One Health Approach", FRONTIERS IN PUBLIC HEALTH, vol. 9, XP093063595, DOI: 10.3389/fpubh.2021.647903 *
PAULA JENDRNY;FRIEDERIKE TWELE;SEBASTIAN MELLER;CLAUDIA SCHULZ;MAREN KöCKRITZ-BLICKWEDE;ALBERTUSDOMINICUSMARCELLINUSERAS OSTE: "Scent dog identification of SARS-CoV-2 infections in different body fluids", BMC INFECTIOUS DISEASES, BIOMED CENTRAL LTD, LONDON, UK, vol. 21, no. 1, 27 July 2021 (2021-07-27), London, UK , pages 1 - 14, XP021294339, DOI: 10.1186/s12879-021-06411-1 *

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