WO2023063545A1 - Multi-pipette provided with multiple independent chambers - Google Patents

Abstract

The present invention relates to a multi-pipette provided with multiple independent chambers and, more specifically, to a multi-pipette provided with multiple independent chambers, the multi-pipette having multiple air inlets and operation chambers corresponding thereto, so that the same amount of sample can be injected into multiple nozzle grooves simultaneously and again discharged simultaneously.

Description

Multi-pipette equipped with a plurality of independent chambers

The present invention relates to a multi-pipette equipped with a plurality of independent chambers, and more particularly, to have a plurality of air inlets and corresponding operation chambers to simultaneously inject equal amounts of samples into a plurality of nozzle grooves, It relates to a multi-pipette provided with a plurality of independent chambers to simultaneously eject the same.

Molecular diagnosis, as one of the in vitro diagnoses, is a method of detecting the cause of the disease and infection by detecting nucleic acids (DNA and RNA or variants thereof) of bacteria and viruses that cause disease.

This molecular diagnosis process largely consists of three steps, in order: a pretreatment process to obtain pure DNA or RNA from cells, a gene amplification process using polymerase chain reaction (PCR), and analysis of amplification products to determine the cause of disease and infection Including the process of detecting whether

In other words, in order to detect the cause of a disease or infection using a very small amount of a gene, it is necessary to amplify the gene, and this Polymerase Chain Reaction (PCR) repeatedly heats and cools the genetic material to generate specific bases of nucleic acid. It is a technology that can exponentially amplify genetic material having a specific nucleotide sequence region by serially copying a region having a sequence.

On the other hand, a plate-type biochip (microchip) has recently been used for polymerase chain reaction, and as shown in FIG. It is composed of a plurality of reagent chambers, an inlet for injecting a fixed amount of sample into each chamber, and a vent hole formed on one side of each chamber. to lose

In this case, the injection of the sample is generally performed using a pipette, etc., and the pipette injects the sample one by one into each inlet using a nozzle tip provided at the bottom of the pipette.

That is, in the case of a conventional pipette, the process is cumbersome as it is made to inject samples one by one into a plurality of inlets. However, when there are many objects to be inspected, there is a problem of giving considerable fatigue to the operator.

The present invention was created to solve the problems of the prior art, and the problem to be solved in the present invention is to be configured to have a plurality of air inlets and corresponding operation chambers, so that an equal amount of sample is supplied to a plurality of nozzle grooves. The present invention relates to a multi-pipette provided with a plurality of independent chambers capable of simultaneously injecting and discharging them simultaneously.

The present invention is doedoe extended in the vertical direction with an empty inside, the housing 100 configured in the form of an open top and bottom; A plurality of air inlets 213a are formed at the lower portion, and receiving grooves 212 are configured to independently receive air introduced through each of the air inlets 213a, and the air inlets 213a are connected to the housing 100. an operation chamber 200 provided on the inner lower side of the housing 100 so as to be exposed to the outside of the lower portion of the; A plurality of nozzle grooves 410 corresponding to the air inlet 213a are formed through the nozzle grooves 410 in the vertical direction so that each nozzle groove 410 communicates with each air inlet 213a. A nozzle tip 400 into which the lower side of the chamber 200 is fitted; And a piston provided on the inner upper side of the housing 100 so that the lower end protrudes into the receiving groove 212 and the upper end is exposed upward of the housing 100 so as to slide vertically by an external force. Part 300; characterized in that it includes.

At this time, the operation chamber 200 is composed of a chamber body 211 extending in the vertical direction and a hole formed downward through the upper surface of the chamber body 211, but a plurality of accommodating grooves provided to form an independent space from each other. (212) and an air inlet (213a) formed at the lower end of the chamber unit 210 including an operation nozzle 213 provided in each receiving groove 212 so that the upper end communicates with the receiving groove 212; And the lower end of the piston shaft 310 of the piston part 300 is fastened through the upper end side, and in this state, the upper end of the receiving groove 212 is made to be in an airtight state so that the upper part of the chamber part 210 To include; a sealing portion 220 provided.

At this time, a fixing groove 211a is formed on the outer surface of the chamber body 211 and is inserted inwardly, and on the lower inner surface of the housing 100, a fixing protrusion inserted into the fixing groove 211a ( 110) is provided.

In addition, the sealing part 220 is made of a material having elasticity, and a plurality of sealing grooves 222 through which the lower end of the piston shaft 320 of the piston part 300 passes are formed on the upper surface of each air inlet 213a. A sealing body 221 formed through the opening to correspond to and provided at an open top of the chamber unit 210; A sealing protrusion 223 protruding downward along the lower circumference of the sealing groove 222 and having a protruding end come into contact with an outer circumferential surface of the piston shaft 320 of the piston unit 300; And a fastening groove 224 made of a groove inserted upward from the lower surface of the sealing body 221, into which the upper end of the chamber part 210 is fitted.

At this time, the sealing groove 222 is made to have a diameter corresponding to the outer circumferential diameter of the piston shaft 320, and a first sealing sphere 222a made of a groove extending downward from the upper surface of the sealing body 221 ); and a second sealing sphere 222b made of a groove extending downward from the lower end of the first sealing sphere 222a, the diameter of which gradually increases from top to bottom and then decreases again.

In addition, a locking protrusion 225 protruding in the central axis direction is provided around the lower end of the sealing body 221, and the locking protrusion 225 is forcibly fitted to the outer circumference of the top of the chamber body 211 so that a locking groove is formed.

On the other hand, the piston part 300 is provided inside the housing 100 in a state in which the piston shaft 320 is inserted and fixed to the lower end side and the upper end penetrates the top of the housing 100 and is exposed to the outside. A piston body 310 to be; And it is made in the form of a rod extending in the vertical direction, the upper end is inserted and fixed to the piston body 310, and the lower end penetrates the sealing part 220 and protrudes into the receiving groove 212 Provided in a state To include; a piston shaft 320 that is.

On the other hand, the piston body 310 has a hooking portion 211a having a cross-sectional area larger than the area of the opening at the top of the housing 100 formed on the upper side of the piston body 310 so that the lower side protrudes into the housing 100. A handle portion 311 that is fastened to be slidably movable in the vertical direction in the state; And a shaft fixing part made in the form of a column extending in the vertical direction, the upper end of which is fastened to the lower end of the handle part 311 in a state where a plurality of shaft grooves into which the piston shaft 320 is inserted and fixed are formed at the lower end ( 312); should be included.

On the other hand, the housing 100 is protruded from the inner wall of the housing 100 toward the center, and the protruding end is formed to correspond to the outer surface of the piston body 310 so as to move upward from the fixing protrusion 110. A piston fixing part 120 is further provided, and the shaft fixing part 312 is provided inside the housing 100 so as to be vertically sliding by the piston fixing part 120 .

On the other hand, the shaft fixing portion 312, the top stopper (312b) protrudes outward from the top circumference; And a lower stopper 312c protruding outward from the lower circumference; further comprising the piston so that the piston fixing part 120 is positioned between the upper stopper 312b and the lower stopper 312c. The body 310 is fastened to the inside of the housing 100.

According to the present invention, it is configured to have a plurality of air inlets and operation chambers corresponding thereto, so that samples can be simultaneously injected into a plurality of nozzle grooves and discharged at the same time.

In addition, as the plurality of operation chambers are configured to inflow and discharge air while applying an external force of equal magnitude, the same amount of sample is accommodated in each nozzle groove, thereby equal amount of sample to the plurality of targets of the PCR kit is supplied so that the target and the sample can sufficiently react.

In addition, according to the present invention, since the nozzle tip of the pipette is configured to be easily detachable, the nozzle tip can be easily changed and used when the sample is changed, thereby improving usability.

1 is a schematic diagram of a conventional biochip.

2 is a perspective view of a multi-pipette equipped with a plurality of independent chambers according to the present invention;

3 is an exploded perspective view of a multi-pipette according to the present invention;

4 is a cross-sectional view of a multi-pipette according to the present invention.

5 is a cross-sectional view of an operation chamber applied to the present invention.

Figure 6 is an enlarged view of area A of Figure 4 according to the present invention, a cross-sectional view of the sealing portion.

Figure 7a is a cross-sectional view of a nozzle tip applied to the present invention.

Figure 7b is a cross-sectional view of an embodiment of separating the nozzle tip applied to the present invention.

8 is a cross-sectional view of a piston unit applied to the present invention.

*Detailed description of the main symbols in the drawing*

100: housing 110: fixing protrusion

120: piston fixing part 130: shaft support part

131: seating groove

200: operating chamber 210: chamber unit

211: chamber body 211a: fixing groove

212: receiving groove 213: operating nozzle

213a: air inlet 220: sealing part

221: sealing body 222: sealing groove

222a: first sealing hole 222b: second sealing hole

223: sealing protrusion 224: fastening groove

225: stumbling block

300: piston part 310: piston body

311: handle part 311a: hooking part

312: shaft fixing part

312b: top stopper 312c: bottom stopper

320: piston axis

400: nozzle tip 410: nozzle groove

H1: sample inlet H2: vent hole

R: target reagent

Hereinafter, preferred embodiments of the present invention will be described in more detail with reference to the accompanying drawings.

The present invention relates to a multi-pipette equipped with a plurality of independent chambers, and more particularly, to have a plurality of air inlets and corresponding operation chambers to simultaneously inject equal amounts of samples into a plurality of nozzle grooves, It relates to a multi-pipette provided with a plurality of independent chambers to simultaneously eject the same.

As shown in FIGS. 2 and 3 , the present invention includes a housing 100 , an operation chamber 200 , a nozzle tip 400 and a piston part 300 .

The housing 100 is configured to form the outer shape of the pipette, and extends vertically with an empty inside, but has an open top and bottom.

At this time, the housing 100 is formed in a form in which a pair of bodies formed symmetrically with each other are assembled and fastened by protrusions and grooves.

On the other hand, as shown in FIGS. 4 and 5, the operation chamber 200 is for accepting and discharging samples into the nozzle tip 400, and has a plurality of air inlets 213a formed at the bottom thereof. The housing ( 100) to be provided on the inner lower side.

To this end, the operation chamber 200 includes a chamber part 210 and a sealing part 220 .

The chamber part 210 is composed of a chamber body 211 extending in the vertical direction and a hole formed downward through the upper surface of the chamber body 211, but a plurality of accommodating grooves 212 provided to form a space independent of each other. and operation nozzles 213 provided in each receiving groove 212 so that the upper end communicates with the receiving groove 212 with the air inlet 213a formed at the lower end.

That is, the operating nozzle 213 is formed in communication with the receiving groove 212 so that air is introduced into the receiving groove 212 through the air inlet 213a or, conversely, is expelled from the receiving groove 212 to the outside. allow it to be released.

As described above, the chamber unit 210 is formed in a form in which the number of operating nozzles 213 corresponding to the plurality of accommodating grooves 212 is provided to correspond to each accommodating groove 212, and each operating nozzle 213 The air introduced through is allowed to be accommodated in each corresponding receiving groove 212 .

At this time, the operation nozzle 213 is formed in a shape in which the outer diameter gradually narrows downward, so that the nozzle tip 400 to be described later can be easily fitted.

On the other hand, a fixing groove 211a is formed on the outer surface of the chamber body 211 and is inserted inwardly, and a fixing protrusion 110 inserted into the fixing groove 211a is formed on the lower inner surface of the housing 100. As it is provided, the operation chamber 200 can maintain a stable state fixed inside the housing 100 .

At this time, it is preferable that two or more pairs of fixing grooves 211a and fixing protrusions 110 are spaced apart in the vertical direction so that the upper and lower ends of the chamber body 211 can be fixed.

On the other hand, as shown in FIG. 6, the sealing part 220 is fastened through the lower end of the piston part 300 through the upper end, and in this state, the upper end of the receiving groove 212 is airtight. It is made so that it is provided on the upper part of the chamber part 210, and includes a sealing body 221, a sealing protrusion 223 and a fastening groove 224.

The sealing body 221 is made of a material having elasticity, so that a plurality of sealing grooves 222 through which the lower end of the piston shaft 320 of the piston part 300 passes on the upper surface correspond to each air inlet 213a It is formed through and provided at the open top of the chamber part 210 .

At this time, the sealing groove 222 includes a first sealing sphere 222a and a second sealing sphere 222b.

The first sealing sphere 222a has a diameter corresponding to the outer circumferential diameter of the piston shaft 320 and is formed of a groove extending downward from the upper surface of the sealing body 221 .

In addition, the circumference of the upper end of the first sealing sphere 222a is formed in a rounded or tapered shape, so that when the piston shaft 320 is fastened, it can guide its entry.

Meanwhile, the second sealing sphere 222b is made of a groove extending downward from the lower end of the first sealing sphere 222a, and the diameter gradually increases from top to bottom and then decreases again.

In detail, the second sealing sphere 222b has an upper end in communication with the first sealing sphere 222a and a lower end of a groove passing through the lower part of the sealing body 221, and an inner circumferential surface thereof in an outer circumferential direction It is made in a concave round shape.

Meanwhile, the sealing protrusion 223 protrudes downward along the lower circumference of the sealing groove 222, and the protruding end comes into contact with the outer circumferential surface of the piston shaft 320.

In detail, the sealing protrusion 223 protrudes obliquely in the direction of the central axis so that the protruding end comes into close contact with the outer circumferential surface of the piston shaft 320 so that the mutual components are airtight.

On the other hand, the fastening groove 224 is made of a groove inserted upward from the lower surface of the sealing body 221, so that the upper end of the chamber portion 210 is fitted.

At this time, a locking protrusion 225 protruding in the central axis direction is provided around the lower end of the sealing body 221, and the locking protrusion 225 is forcibly fitted to the upper outer circumference of the chamber body 211. to form a groove.

In addition, the protruding protrusion 225 is gradually formed into a pointed shape with a protruding end.

According to the configuration described above, the sealing part 220 and the chamber part 210 are fastened in an airtight state, and at the same time, the piston part 300 is also configured to slide up and down in an airtight state, so that the chamber part While the piston shaft 320 is moved up and down while the inside of the 210 is in a vacuum state, the sample can be introduced into the nozzle tip 400 .

At this time, in the present invention, the outer circumferential surface of the piston shaft 320 penetrated through the sealing part 220 is supported by the first sealing sphere 222a and the sealing protrusion 223, and in this state, the up and down sliding operation This is done, that is, as the piston shaft 320 is operated in a state in which it is stably supported by a pair of protrusions disposed in the vertical direction, the airtightness between the mutual components is made more stable.

In addition, the second sealing hole 222b does not come into close contact with the outer circumferential surface of the piston shaft 320, and a predetermined space is formed around the outer circumferential surface of the piston shaft 320, so that the piston shaft 320 is moved up and down. It is possible to prevent a phenomenon in which the sealing part 220 is deformed by an external force generated at the same time.

On the other hand, the nozzle tip 400 is for substantially accommodating the sample, and as shown in FIG. 7A, a plurality of nozzle grooves 410 corresponding to the air inlet 213a are vertically formed through each The lower side of the operation chamber 200 is fitted into the nozzle groove 410 so that the nozzle groove 410 communicates with each air inlet 213a.

In detail, the nozzle tip 400 is formed such that a plurality of nozzle grooves 410 are coaxial with the air inlet 213a, so that the operating nozzle 213 is located at the upper end of the nozzle groove 410. The nozzle tip 400 can be fastened to the lower end of the housing 100 as it is fitted through the .

At this time, the nozzle groove 410 is made to have a larger volume than the operating nozzle 213, and when the nozzle tip 400 is fastened to the lower end of the operating chamber 200, the operating nozzle 213 A free space is formed in the nozzle groove 410 at the lower end of the.

Meanwhile, the nozzle tip 400 is detachably made.

In detail, as shown in FIG. 7A, the upper circumference of the nozzle tip 400 is further provided with a wing part 420 protruding outwardly. At this time, the wing part 420 is the housing ( 100) to protrude outward from the lower end side.

Accordingly, when force is applied to the nozzle tip 400 from the top to the bottom of the wing 420, the nozzle tip 400 can be separated while the fitting with the operation chamber 200 is released. .

At this time, the nozzle tip 400 can be separated by the operator's hand. Since the nozzle tip 400 is used while being drawn into the container containing the sample, the tip of the nozzle tip 400 is buried with the sample, When the operator separates the sample by hand, the sample is buried in the hand, and this may cause a problem of contamination when replacing another nozzle tip 400.

Therefore, in the present invention, in order to easily separate the nozzle tip 400 using a separate tool, as shown in FIG. 7B, a predetermined distance between the upper end of the wing 420 and the lower end of the housing 100 A space of , and after inserting a plate-shaped tool into the space, the nozzle tip 400 can be separated by applying an external force to the tool.

Meanwhile, as shown in FIG. 8 , the piston part 300 slides up and down by an external force in a state where the lower end protrudes into the receiving groove 212 and the upper end is exposed upward of the housing 100. It is provided on the inner upper end of the housing 100 to be movable.

The piston part 300 includes a piston body 310 and a piston shaft 320.

The piston body 310 is provided inside the housing 100 in a state in which the piston shaft 320 is inserted and fixed to the lower side, and the upper end penetrates the top of the housing 100 and is exposed to the outside.

At this time, the piston body 310 includes a handle part 311 and a shaft fixing part 312.

The handle part 311 has a hooking part 211a having a cross-sectional area larger than the area of the opening at the top of the housing 100 formed on the upper side of the handle part 311 so that the lower side protrudes into the housing 100. It is fastened to be able to slide in the direction.

In detail, the lower end of the handle part 311 passes through the open top of the housing 100 and is inserted and fastened inwardly, and in this state, when the upper end of the handle part 311 is pressed, the handle part ( 311) slides downward, and at this time, further entry is restricted by the hooking portion 211a of the handle portion 311.

On the other hand, the shaft fixing part 312 is made in the form of a pillar extending in the vertical direction, and the upper end is the lower end of the handle part 311 in a state in which a plurality of shaft grooves into which the piston shaft 320 is inserted and fixed are formed at the lower end side. Fastening is provided on the side.

At this time, the shaft fixing part 312 and the handle part 311 are detachably fastened by protrusions and grooves so that they can be separated if necessary.

Meanwhile, the number of the shaft grooves corresponding to the air inlets 213a is provided to be located on the same vertical axis.

On the other hand, the shaft fixing part 312 is provided inside the housing 100 to be able to slide up and down by the piston fixing part 120 provided on the inner wall of the housing 100 .

In detail, the piston fixing part 120 protrudes from the inner wall of the housing 100 toward the center, and the protruding end is formed to correspond to the outer surface of the piston body 310 so that the fixing protrusion 110 It is provided in the upper direction, and the shaft fixing part 312 is configured to further include an upper stopper 312b protruding outward from the upper circumference and a lower stopper 312c protruding outward from the lower circumference. The piston body 310 is fastened to the inside of the housing 100 so that the piston fixing part 120 is located between the stopper 312b and the lower stopper 312c.

Accordingly, the movement of the piston body 310 upward and downward is restricted by the upper stopper 312b and the lower stopper 312c.

On the other hand, the piston shaft 320 is formed in the form of a rod extending in the vertical direction, the upper end is inserted and fixed into the shaft groove, and the lower end penetrates the sealing portion 220 and protrudes into the receiving groove 212 to be made available.

At this time, the piston shaft 320 allows the vertical sliding movement to be supported by the shaft support 130 provided inside the housing 100 .

In detail, the shaft support part 130 protrudes from the inner wall of the housing 100 toward the center, and at the protruding end, a seating groove 131 through which the piston shaft 320 is inserted is formed, and the sealing It is provided between the part 220 and the piston fixing part 120.

At this time, the shaft support part 130 is provided so that its lower surface comes into contact with the upper surface of the sealing part 220 to more stably support the vertical movement of the piston shaft 320 .

Meanwhile, an elastic body (not shown) for elastically supporting the piston body 310 upward is provided between the piston body 310 and the sealing part 220 .

In detail, the elastic body is composed of a spring that is compressed when the piston body 310 is pressed downward and tension is restored again when the external force applied to the piston body 310 is removed.

At this time, one of the elastic bodies may be provided to surround the circumferences of the plurality of piston shafts 320, or a plurality of elastic bodies having the same elastic force may be provided to surround the circumferences of each piston shaft 320.

According to the configuration described above, when the piston unit 300 presses the upper end of the piston body 310, the piston shaft 320 is moved downward by interlocking with this, and accordingly, the lower end of the piston shaft 320 While being gradually drawn into the receiving groove 212, the air accommodated in the receiving groove 212 can be discharged through the air inlet 213a.

At this time, in the present invention, a plurality of piston shafts 320 are fastened so as to interlock with the operation of the piston body 310, and each piston shaft 320 is horizontally symmetrical with respect to the center of the piston body 310. As such, when an external force is applied to the piston body 310, the same force is applied to each piston shaft 320 so that air is discharged from each receiving groove 212 to the outside.

On the other hand, when the nozzle tip 400 is submerged in the container containing the sample in the above state, and the external force applied to the piston axis 320 is removed in this state, the elastic body is restored while the piston axis ( 320) is moved upward, and accordingly, the sample is introduced into the nozzle tip 400 and accommodated in the free space of the plurality of nozzle grooves 410, respectively.

At this time, since the same force is continuously applied to the piston shaft 320, the same amount of sample can be accommodated in each free space.

Then, when the piston shaft 320 is pressed again, an equal amount of samples accommodated in the free space of each nozzle groove 410 is discharged to the outside, so that equal amounts of samples are supplied to a plurality of targets of the PCR chip. Thus, the target and the sample can sufficiently react.

In addition, in the present invention, since the sample is introduced into the nozzle tip 400, when a different sample needs to be used, a simple process of separating and discharging the nozzle tip 400 and then fastening a new nozzle tip 400. This makes it easy to handle a variety of samples.

Although preferred embodiments of the present invention have been described above, the scope of the present invention is not limited thereto, and it should be understood that the scope of the present invention extends to those within the scope substantially equivalent to the embodiments of the present invention. Various modifications and implementations are possible by those skilled in the art to which the invention pertains without departing from the spirit of the invention.

Claims (10)

A housing 100 extending vertically with an empty interior and having an open top and bottom; A plurality of air inlets 213a are formed at the lower portion, and receiving grooves 212 are configured to independently receive air introduced through each of the air inlets 213a, and the air inlets 213a are connected to the housing 100. an operation chamber 200 provided on the inner lower side of the housing 100 so as to be exposed to the outside of the lower portion of the; A plurality of nozzle grooves 410 corresponding to the air inlet 213a are formed through the nozzle grooves 410 in the vertical direction so that each nozzle groove 410 communicates with each air inlet 213a. A nozzle tip 400 into which the lower side of the chamber 200 is fitted; And a piston provided on the inner upper side of the housing 100 so that the lower end protrudes into the receiving groove 212 and the upper end is exposed upward of the housing 100 so as to slide vertically by an external force. A multi-pipette equipped with a plurality of independent chambers, characterized in that it comprises a; part (300). The method according to claim 1, wherein the operation chamber (200) is composed of a chamber body (211) extending in a vertical direction and a hole formed downward through a top surface of the chamber body (211), and a plurality of holes are provided to form an independent space. A chamber unit 210 including operating nozzles 213 provided in each receiving groove 212 so that the upper end communicates with the receiving groove 212 with the receiving groove 212 and the air inlet 213a formed at the lower end thereof. ; And the lower end of the piston shaft 310 of the piston part 300 is fastened through the upper end side, and in this state, the upper end of the receiving groove 212 is made to be in an airtight state so that the upper part of the chamber part 210 A multi-pipette equipped with a plurality of independent chambers, characterized in that it includes; a sealing unit 220 provided. The method according to claim 2, on the outer surface of the chamber body (211), a fixing groove (211a) is formed in the inner direction, and on the lower inner surface of the housing (100), the fixing groove (211a) is inserted A multi-pipette having a plurality of independent chambers, characterized in that a fixing protrusion 110 is provided. The method according to claim 2, wherein the sealing part 220 is made of a material having elasticity, and a plurality of sealing grooves 222 through which the lower end of the piston shaft 320 of the piston part 300 passes through the upper surface of each air inlet a sealing body 221 formed to correspond to (213a) and provided at an open top of the chamber part 210; A sealing protrusion 223 protruding downward along the lower circumference of the sealing groove 222 and having a protruding end come into contact with an outer circumferential surface of the piston shaft 320 of the piston unit 300; and a fastening groove 224 formed of a groove inserted upward from the lower surface of the sealing body 221 and into which the upper end of the chamber part 210 is fitted. A plurality of independent chambers are provided multi-pipette. The method according to claim 4, wherein the sealing groove 222 is made to have a diameter corresponding to the outer circumferential diameter of the piston shaft 320, the first sealing consisting of a groove extending downward from the upper surface of the sealing body 221 sphere 222a; and a second sealing sphere (222b) made of a groove extending downward from the lower end of the first sealing sphere (222a), the diameter of which gradually increases from top to bottom and then decreases again. Multi-pipette equipped with two independent chambers. The method according to claim 4, around the lower end of the sealing body 221, the locking protrusion 225 protrudes in the direction of the central axis is provided, the outer circumference of the upper end of the chamber body 211, the locking protrusion 225 A multi-pipette provided with a plurality of independent chambers, characterized in that a locking groove is formed into which is forcibly fitted. The method according to claim 2, wherein the piston part 300, the piston shaft 320 is inserted and fixed to the lower side, the upper end of the housing 100 in a state exposed to the outside through the top of the housing 100 A piston body 310 provided therein; And it is made in the form of a rod extending in the vertical direction, the upper end is inserted and fixed to the piston body 310, and the lower end penetrates the sealing part 220 and protrudes into the receiving groove 212 Provided in a state A multi-pipette equipped with a plurality of independent chambers, characterized in that it comprises a; piston shaft 320. The method according to claim 7, wherein the piston body (310) has a hooking portion (211a) having a larger cross-sectional area than the area of the opening at the upper end of the housing (100) formed on the upper side, so that the lower side extends to the inside of the housing (100). A handle portion 311 coupled to be slidably moved in the vertical direction while protruding; And a shaft fixing part made in the form of a column extending in the vertical direction, the upper end of which is fastened to the lower end of the handle part 311 in a state where a plurality of shaft grooves into which the piston shaft 320 is inserted and fixed are formed at the lower end ( 312); A multi-pipette comprising a plurality of independent chambers. The piston fixing part 120 of claim 8, wherein the housing 100 protrudes from the inner wall of the housing 100 toward the center, and the protruding end is formed to correspond to the outer surface of the piston body 310. Is further provided, and the shaft fixing part 312 is provided with a plurality of independent chambers, characterized in that provided inside the housing 100 to enable up and down sliding movement by the piston fixing part 120 multi pipette. The method according to claim 9, wherein the shaft fixing part 312, the top stopper (312b) protrudes outward from the top circumference; And a lower stopper 312c protruding outward from the lower circumference; further comprising the piston so that the piston fixing part 120 is positioned between the upper stopper 312b and the lower stopper 312c. A multi-pipette having a plurality of independent chambers, characterized in that the body 310 is fastened to the inside of the housing 100.

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