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WO2022263364A1 - Flacon pour la cryoconservation d'échantillons biologiques multiples, base de montage associée et kit de cryoconservation comprenant un tel flacon et une telle base de montage, et procédé mis en œuvre avec un tel kit - Google Patents

Flacon pour la cryoconservation d'échantillons biologiques multiples, base de montage associée et kit de cryoconservation comprenant un tel flacon et une telle base de montage, et procédé mis en œuvre avec un tel kit Download PDF

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Publication number
WO2022263364A1
WO2022263364A1 PCT/EP2022/066007 EP2022066007W WO2022263364A1 WO 2022263364 A1 WO2022263364 A1 WO 2022263364A1 EP 2022066007 W EP2022066007 W EP 2022066007W WO 2022263364 A1 WO2022263364 A1 WO 2022263364A1
Authority
WO
WIPO (PCT)
Prior art keywords
vial
container
mounting base
reception
volume
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2022/066007
Other languages
English (en)
Inventor
Antoine Gautier
Xianghua Zhang
François CHEVIRE
Patricia LEROYER
Olivier Loreal
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Universite de Rennes 1
Institut National des Sciences Appliquees de Rennes
Institut National de la Sante et de la Recherche Medicale INSERM
Ecole Nationale Superieure de Chimie de Rennes
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite de Rennes 1
Institut National des Sciences Appliquees de Rennes
Institut National de la Sante et de la Recherche Medicale INSERM
Ecole Nationale Superieure de Chimie de Rennes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Universite de Rennes 1, Institut National des Sciences Appliquees de Rennes, Institut National de la Sante et de la Recherche Medicale INSERM, Ecole Nationale Superieure de Chimie de Rennes filed Critical Centre National de la Recherche Scientifique CNRS
Publication of WO2022263364A1 publication Critical patent/WO2022263364A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/14—Mechanical aspects of preservation; Apparatus or containers therefor
    • A01N1/146—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving
    • A01N1/147—Carriers for immersion in cryogenic fluid for slow freezing or vitrification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/06—Test-tube stands; Test-tube holders
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/50—Cryostats

Definitions

  • TITLE Vial for the cryopreservation of multiple biological samples, associated mounting base and cryopreservation kit comprising such a vial and such a mounting base, and method implemented with such a kit
  • the present invention relates to a vial for the cryopreservation of biological samples, to an associated mounting base and to a cryopreservation kit comprising such a vial and such a mounting base.
  • the present invention also concerns a method implemented with such a cryopreservation kit.
  • each biological sample is placed separately in a respective cryopreservation vial, made out of a material resistant both to cold temperatures and to sudden temperature changes, such as polypropylene.
  • Each vial is referenced with a suitable coding system such as a bar code or physical marker, then several vials are placed together onto a rack, and then the rack is placed in a frozen storage cabinet.
  • a suitable coding system such as a bar code or physical marker
  • the operator extracts the corresponding rack from the storage cabinet, identifies the corresponding vial, opens the vial then retrieves the sample.
  • the operator must operate quickly in order to prevent the sample from thawing, which is not convenient since the operator usually wears protection gloves or mittens.
  • Each vial usually has a standardized shape with a given capacity, which is compatible with the rack.
  • US-2019/092555-A1 describes, for example, vials having the shape of elongated tubes.
  • WO-2016/178635-A1 describes vials having each a smaller length compared to elongated tubes, in order to save preserve biological samples with small sizes.
  • Several vials can be stacked together for easier handling.
  • the problem remains for samples whose size is still much smaller than a fraction of the size of the vial, since it is not possible to reduce indefinitely the size of the vial, as the handling of small vials while wearing gloves is not practical.
  • CN-206 565 163-U describes, for example, a vial for the cryopreservation of biological samples.
  • the vial comprises a tube and a cap.
  • the tube comprises inner partitions that divide the inner volume of the tube into several chambers.
  • US-2007/104617-A1 describes, for example, a vial for the cryopreservation of biological samples.
  • This vial comprises a container closed by a threaded cap.
  • the container comprises external protrusions configured to cooperate with a receptacle of a tube rack, in order to lock the container in rotation when the container is received in a receptacle.
  • the cap comprises external protrusions configured to cooperate with a tool, enabling an operator to single handedly de-cap or re-cap the container received in a receptacle.
  • aspects of the invention pertains to a vial for the cryopreservation of biological samples, this vial comprising:
  • a container made in one piece, defining an inner volume and an opening provided with a thread
  • a threaded cap which cooperates with the thread of the opening so as to selectively close the inner volume, in a sealed configuration of the vial, or allow the access to the inner volume, in an unsealed configuration of the vial.
  • the container includes at least one inner partition, configured to divide the inner volume in at least two separated chambers, each chamber being accessible from the opening.
  • each vial can be stored within each vial, with one sample stored in a respective chamber.
  • a ratio of unused volume within each vial is reduced, while each vial remains big enough to be easily handled by an operator, particularly while wearing gloves or mittens.
  • the ratio of unused volume is four time smaller compared to known container according to the background art, such known container having the same inner volume, in one piece, but no inner partition.
  • the vial can be sealed or unsealed at will, the separated chambers being readily accessible from the opening when the cap is removed from the container, so that after a user retrieves a sample from one of the inner chambers, the user can seal the vial and place the vial back in the storage cabinet, which is convenient.
  • such a vial may incorporate one or more of the following features, considered alone or according to any technically allowable combination:
  • each inner partition contacts the threaded cap, so that each chamber is sealed and is separated from the other chambers.
  • the container comprises a bottom surface arranged opposite to the opening, whereas:
  • the container is configured to be inserted in a reception volume of a mounting base along an insertion axis, the reception volume having a bottom wall with a reception relief,
  • the bottom surface has a positioning relief, configured to cooperate with the reception relief
  • the positioning relief is non-circular, so that the container is locked in rotation relative to the mounting base when the container fully inserted in the reception volume.
  • the positioning relief has a shape configured so that the cooperation of the positioning relief with the reception relief is possible with only one angular position of the container relative to the mounting base around the insertion axis.
  • the container has an outer surface, whereas the outer surface comprises marks, which are distributed on the outer surface for identifying each chamber, when the vial is in the sealed configuration and when the vial is in the unsealed configuration.
  • the outer surface of the container comprises a peripheral surface, whereas the marks are reliefs, in particular parallel ribs:
  • the threaded cap has an external shape configured to cooperate with a tool, in order to screw / unscrew the cap onto / from the container by rotating the threaded cap relative to the container around a main axis of the container and wherein, preferably:
  • the threaded cap comprises a closing portion, which is orthogonal to the main axis when the cap cooperates with the thread of the opening, • the closing portion comprises a recess, which is arranged in the closing portion and is oriented opposite to the inner volume in the sealed configuration of the vial,
  • the recess is configured to cooperate with a tool to screw / unscrew the cap onto / from the container by rotating the threaded cap relative to the container around the main axis.
  • the invention also concerns a mounting base, comprising a plate with a top surface, and at least one recessed reception volume arranged in the plate, wherein each reception volume opens on the top surface and is configured to receive one container of a vial as previously defined.
  • such a mounting base may incorporate one or more of the following features, considered alone or according to any technically allowable combination:
  • the base is made out of a material with high thermal capacity and high thermal conductivity, preferably from a stainless metal, for example from stainless steel, whereas the mounting base is configured to cool the separated chambers of a container after said container is fully received in the corresponding reception volume, after being cooled down prior to the insertion of said container in the corresponding reception volume.
  • Each reception volume comprises a peripheral wall, which is configured to cover at least 50% of a peripheral surface of the container when said container is fully inserted in the corresponding reception volume, preferably at least 80%.
  • Each reception volume defines an insertion axis and is configured to receive a container of a vial as previously defined, whereas each reception volume comprises a bottom wall with a reception relief, which is configured to cooperate with the positioning relief protruding from the bottom surface of the container, so that said container can be fully inserted in the corresponding reception volume with only one possible angular orientation of the container around the insertion axis.
  • the top surface comprises sets of marks, for example a set of numbers, one set of marks being arranged around each reception volume, whereas each set of marks is arranged so that, when a vial is fully inserted in the corresponding reception volume, each chamber is facing a corresponding mark of the set of marks.
  • the invention concerns further a cryopreservation kit for the handling of biological samples, the cryopreservation kit comprising at least one vial as previously defined and the mounting base as previously defined, wherein each reception volume of the mounting base is configured to receive the container of one vial.
  • the invention also concerns a method for the preservation of biological samples, the method being implemented with a cryopreservation kit as previously defined and comprising the following steps:
  • figure 1 is a perspective view of a cryopreservation kit according to the invention and comprising a mounting base and two vials, shown respectively in a sealed and in an unsealed configuration;
  • figure 2 is an enlarged perspective view, along arrow II on figure 1 , of a detail of the mounting base of Figure 1 ;
  • figure 3 is a perspective view of a vial of the cryopreservation kit of figure 1 , shown in the unsealed configuration, this vial being according to the invention;
  • figure 4 is a perspective view according to another angle, along arrow IV on figure 3, of the container of the vial of figure 3, and
  • figure 5 is an enlarged cross-sectional view, along section V-V on figure 1 , of the cryopreservation kit of Figure 1 .
  • a cryopreservation kit 10 according to the invention is shown on figure 1 .
  • the cryopreservation kit 10 also simply called “kit” 10 in short, comprises a mounting base 20 and at least one vial 100.
  • the mounting base 20 comprises a plate 22 with a top surface 24. At least one recessed reception volume 26 is arranged in the base 22. Each reception volume 26 is configured to receive one vial 100.
  • the top surface 24 is preferably flat.
  • the plate 22 has a parallelipedic shape and the top surface 24 has a rectangular shape.
  • the mounting base 20 is usually installed on a support member so that the reception volumes 26 are accessible for an operator.
  • the top surface 24 is facing upwards and is preferably horizontal.
  • the mounting base 20 comprises preferably several reception volumes 26, which are advantageously regularly arranged on the top surface 24.
  • the mounting base 20 comprises twelve reception volumes 26.
  • one of the twelve reception volumes 26 receives a closed vial 100
  • another one of the twelve reception volumes 26 receives an open vial 100
  • the other ten reception volumes 26 are empty.
  • All reception volumes 26 of the mounting base 20 work in a similar way.
  • all the reception volumes 26 of the same mounting base 20 are identical to each other.
  • the reception volume 26 has a cylindrical shape centered on an insertion axis A26 with a circular cross-section. Each insertion axis A26 is preferably orthogonal to the top surface 24. Each reception volume 26 opens on the top surface 24 and is configured to receive one vial 100.
  • Each reception volume 26 comprises a cylindrical peripheral wall 28 and a bottom wall 30.
  • a reception relief 32 is arranged in each bottom wall 30, each reception relief 32 being configured to cooperate with a vial 100, as explained here below.
  • the reception volumes 26 are preferably regularly arranged on the top surface 24, so that each reception volume 26 can be easily accessed by an operator without interfering with the adjacent reception volumes 26.
  • the twelve reception volumes 26 are arranged in a rectangular array, with four columns 34 and three rows 36 in the illustrated example.
  • the columns 34 and the rows 36 are preferably identified by alphanumeric signs.
  • the four columns 34 are here identified by alphabet letters from “A” to “D”, while the three rows 36 are identified here by a Roman numbers from ⁇ ” to “III”.
  • the top surface 24 comprises several sets of marks 38, one set of marks 38 being arranged around each reception volume 26.
  • Each set of marks 38 comprises here Arabic numbers from 1 to 4, that are arranged radially to the insertion axis A26 around each reception volume 26 so that, when a vial 100 is fully inserted in the corresponding reception volume 26, each mark of the set of marks 38 is facing a corresponding chamber of this vial 100, as explained here below.
  • the set of marks 38 are engravings made onto the top surface 24.
  • the set of marks 38 are printed onto the top surface 24.
  • the top surface 24 is made by a protection layer 40 applied on the plate 22, each reception volume 26 extending through the protection layer 40.
  • the protection layer 40 is made out of cold resistant and non sticky material, preferably made from polymer material, for example from polytetrafluoroethylene, or PTFE in short and also known under the brand name “Teflon”.
  • a vial 100 is illustrated on figure 3.
  • the vial 100 comprises a container 110 and a cap 150.
  • the container 110 is made in one piece, that is monobloc, from a material suitable for long term cryopreservation, such as polypropylene.
  • Each container 110 presents a cylindrical shape extending along a main axis A110 and delimits an inner volume 112 with an opening 114.
  • each container 110 as an external shape that remains compatible with the handling tools used to handle known cryopreservation vials.
  • handling tools may include racks, for example used to store cryopreservation vials within a storage cabinet, tongs, etc.
  • the opening 114 is located within a plane that is orthogonal to the main axis A110.
  • the main axis A110 is considered to be vertical, with the opening 114 facing upwards.
  • the container 110 comprises an outer surface 116, which is opposite to the inner volume 112.
  • the outer surface 116 comprises a peripheral surface 118 and a bottom surface 120.
  • the peripheral surface 118 is globally cylindrical with a circular section around the main axis A110 and extends along the main axis A110, while the bottom surface 120 is arranged opposite to the opening 114 and perpendicular to the main axis A110.
  • Each container 110 is configured to be inserted in one reception volume 26 of the mounting base 20 along the corresponding insertion axis A26, in an inserted configuration of the container 110.
  • a vial 100 is also said to be “in the inserted configuration” when the container 110 of this vial 100 is in the inserted configuration.
  • the peripheral surface 118 is facing the peripheral wall 28
  • the bottom surface 120 is facing the bottom wall 30
  • the main axis A110 is aligned with the insertion axis A26.
  • the container 110 comprises a positioning relief 122, which is arranged on the bottom surface 120.
  • the positioning relief 122 has a shape that is complementary to the reception relief 32 of the bottom wall 30 and is configured to cooperate with the reception relief 32, preferably by male-female cooperation, in order to increase a contact surface between the container 110 and the plate 22, as explained here below.
  • the positioning relief 122 is a protrusion, which protrudes from the bottom surface 120, so as to avoid encroaching on the inner volume 112 of the container 110, while the reception relief 32 is a recess arranged in the bottom wall 30.
  • the positioning relief 122 has a non-circular shape relatively to the main axis A110 so that, when the positioning relief 122 cooperates with the reception relief 32, the rotation movement of the container 110 relative to the mounting base 20 around the insertion axis A26 is prevented.
  • the container 110 is said to be “fully inserted” in the corresponding reception volume 26 when the positioning relief 122 cooperates with the reception relief 32 and prevents the rotation of the container 110 relative to the base 20 around the insertion axis A26.
  • the positioning relief 122 has a shape configured so that the cooperation of the positioning relief 122 with the reception relief 32 is possible with only one angular position of the container 110 relative to the mounting base 20 around the insertion axis A26.
  • the positioning relief 122 has the shape of a “T” and, as shown on figure 2, the reception relief 32 also has the shape of a “T”.
  • the container 110 includes at least one inner partition 124.
  • Each inner partition 124 is configured to divide the inner volume 112 in at least two separated chambers 126, the two chambers 126 being accessible from the opening 114.
  • Each chamber 126 is configured to receive one biological sample.
  • the biological samples are not represented.
  • Biological samples include samples that are solid at ambient temperatures, such as pieces of vegetal tissue or animal tissue collected by biopsy, or samples that are liquid at ambient temperature, such as blood, plasma, etc.
  • the chambers 126 belonging to the same container 110 may receive biological samples of the same type, or alternatively biological samples of different types.
  • the container 110 comprises two inner partitions 124, which extend radially to the main axis A110 and cross each other orthogonally, so that the inner volume 112 is divided in four separated chambers 126.
  • the separated chambers 126 of a container 110 have the same shape.
  • the outer surface 116 of the container 110 comprises marks 128, which are distributed on the outer surface 116 radially to the main axis A110 in order to identify each chamber 126 when the vial 100 is in the sealed configuration and when the vial 100 is in the unsealed configuration.
  • each mark 128 is aligned, along a direction radial to the main axis A110, with a chamber 126 that it identifies.
  • the marks 128 are made of protruding reliefs 130.
  • the protruding reliefs 130 are ribs, which are arranged on the peripheral surface 118 and parallel to the main axis A110.
  • the number of protruding reliefs 130 is equal to a ranking number of said chamber 126.
  • each container 110 comprises four separated chambers 126 that are ranked from one to four, and the protruding reliefs 130 are arranged in groups from one rib to four ribs, to constitute the marks 128, as shown on figure 4.
  • the marks of the set of marks 38 are arranged around each reception volume 26 so that when a vial 100 is fully inserted in said reception volume 26, each mark of the set of marks 38 is facing one of the chambers 126 of said vial 100 and indicates the ranking number of the corresponding chamber 126.
  • the shape and orientation of the positioning relief 122 and the reception relief 32 are such that, when they cooperate in the fully inserted configuration of the container 110 within the reception volume 26, the mark 128 made of one rib 130 faces the mark ⁇ ” of the set of marks 38, while the mark 128 made of two ribs 130 faces the mark 2 of the set of marks 38, the mark 128 made of three ribs 130 faces the mark “3” of the set of marks 38 and the mark 128 made of four ribs 130 faces the mark “4” of the set of marks 38. This allows identifying the four chambers 126 also when the vial 100 is mounted on the mounting base 20.
  • each container 110 is provided with a thread 132, while the cap 150 comprises a threaded portion 152 that cooperates with the thread 132, so as to selectively close the inner volume 112 in a sealed configuration of the vial 100, as shown on figure 5.
  • the threaded cap 150 is tightly screwed onto the thread 132 of the opening 112.
  • the threaded cap 150 is not mounted on the container 110, and the vial 100 is in an unsealed configuration.
  • the thread 132 is an external thread, that is oriented outward the main axis A110, while the threaded portion 152 comprises an internal thread and an outer surface 153 with a cylindrical shape of a circular section that extends along a cap axis A150.
  • the cap 150 also comprises a closing wall 154, which closes the inner volume 112 in the sealed configuration of the vial 100.
  • the closing wall 154 extends orthogonally to the cap axis A150.
  • the cap axis A150 is aligned with the main axis A110.
  • the closing wall 154 is then orthogonal to the main axis A110 of the container 110.
  • Each inner partition 124 extends up to the opening 114 so that, when the vial 100 in the sealed configuration, each inner partition 124 contacts the closing wall 154 of the cap 150, as shown on figure 5. Thus, each separated chamber 126 is sealed and is separated from the other chambers 126.
  • the top of the container 110 is flat. More precisely, a top surface of the inner partition 124 and a top surface of the peripheral surface 118 are borne by a geometrical plane P112 perpendicular to the main axis A110. On the other hand, a contact surface 156 of the closing wall 154, configured to close the inner volume 112, is also flat and borne by a geometrical plane P156 perpendicular to the cap axis A110.
  • a sealing member is arranged between the container 110 and the cap 150 so as to improve the sealing of each chamber 126 of the vial 100 in the sealed configuration of the vial 100.
  • the sealing member is made out of a material that remains flexible both at ambient temperature and when submitted long term at low temperature, for example in liquid nitrogen at -176°C.
  • the sealing member is made out of PTFE or out of silicone elastomer.
  • the sealing member comprises a flat portion, sandwiched between the inner partitions 124 and the closing wall 154 when the vial 100 is in the sealed configuration, so that each chamber 126 is sealed and separated from the other chambers 126.
  • the sealing member comprises a ring, which is arranged at the periphery of the closing wall 154 and which cooperates with the thread 132 when the vial 100 is in the sealed configuration
  • the cap 150 has an external shape configured to cooperate with a tool, in order to screw / unscrew the cap 150 onto / from the container 110, by rotating the threaded cap 150 relative to the container 110 around the main axis A110 of the container 110.
  • the closing wall 154 is provided with a recess 158, which is oriented opposite to the contact surface 156.
  • the recess 158 is oriented opposite to the inner volume 112.
  • the recess 158 is configured to cooperate with a tool, not shown, to screw/unscrew the cap 150 onto/from the container 110 by rotating the threaded cap 150 relative to the container 110 around the main axis A110.
  • the recess 158 is a groove, which extends radially to the cap axis A150, the groove being configured to cooperate with a tip end of a screw driver.
  • the recess 158 has a different shape, configured to cooperate with different types of tools, for example a cross-headed screw driver, a hexagonal spanner, etc.
  • a first example of method implemented with the cryopreservation kit 10 is described hereinafter.
  • a vial 100, initially in the sealed configuration, is taken out by an operator from a storage cabinet, not shown.
  • the operator selects an empty reception volume 26 of the mounting base 20 and presents the vial 100 with its bottom surface 120 facing the selected reception volume 26, after having aligned the main axis A110 of the container 110 with the insertion axis A26.
  • the operator By approaching the container 110 to the chosen reception volume 26 along the insertion axis A26, the operator inserts the container 110 into the reception volume 26, until the positioning relief 122 of the bottom surface 120 comes into contact with the bottom wall 30.
  • the vial 100 can be further inserted into the reception volume 26, in the fully inserted configuration shown on figure 5.
  • the positioning relief 122 of the container 110 cooperates with the reception relief 32 of the reception volume 26, and the bottom wall 30 is in contact with the bottom surface 120.
  • the container 110 is fully inserted in the corresponding reception volume 26 with only one possible angular orientation of the container 110 around the insertion axis A26.
  • the respective reception reliefs 32 arranged in each reception volume 26 of the mounting base 20 are all oriented in the same direction, so that when a vial 100 is inserted in any one of the reception volumes 26, this vial 100 is always fully inserted with the same angular orientation of the container 110 relative to the mounting base 20.
  • the rotation of the container 110 relative to the mounting base 20 around the insertion axis A26 is prevented.
  • the operator can further use a tool in order to unscrew the cap 150 from the container 110, without having to hold the container 110 with one hand, which is particularly convenient, since the operator usually wears protection gloves in order to manipulate the vial at low temperature.
  • the plate 22 is made out of a material with a high thermal capacity and a high thermal conductivity.
  • high thermal capacity and high thermal conductivity are considered comparatively to the materials used for the racks used to hold cryopreservation tubes taken out of a storage cabinet such as a liquid nitrogen tank.
  • tube holders are traditionally made out of polymer material, such as polypropylene or PTFE.
  • the plate 22 is made out of metal, preferably from stainless metal, for example from stainless steel or from aluminum or one of its alloys.
  • the mounting base 20 is preparatorily cooled below freezing temperature, for example by placing the mounting base 20 in a freezer for a suitable duration, or by placing the mounting base 20 in a suitable box containing carbo-ice or liquid nitrogen.
  • the mounting base 20 is configured to keep frozen the separated chambers 126 of a vial 100 or a container 110 fully received in a corresponding reception volume 26.
  • the mounting base 20 allows keeping the vial 100 and the biological samples contained in this vial frozen for a longer time, as compared to when the operator holds directly the vial 100 with its gloves. The operator does not have to hurry in order to avoid these biological samples thawing, which is very convenient.
  • a second example of method implemented with the cryopreservation kit 10 is now described, namely a method for the cryopreservation of biological samples during a manipulation of at least one biological sample.
  • an operator cools the mounting base 20, for instance by storing it in a freezer for a given period of time.
  • the operator installs the mounting base 20 on a support member, so that the reception volumes 26 of this mounting base 20 are accessible for the operator.
  • the operator After the operator is done manipulating the biological samples contained in a vial 100, the operator stores this vial back in the storage cabinet. Then, the operator places the mounting base 20 base back in the freezer, waiting for the next use. Alternatively, if the operator needs to work further with the same biological samples, the operator preferably seals each container 110 in its corresponding reception volume 26, then places the mounting base 20 back in the freezer, with all the vials 100 left in their respective reception volumes 26.
  • the cooling of the separated chambers 126 is done through the bottom surface 120 in contact with the bottom wall 30, and also through the peripheral surface 118 facing the peripheral wall 28.
  • the container 110 may protrude upwardly with respect to the top surface 24, depending on the respective lengths of the reception volume 26 and the container 110 along axes A26 and A110.
  • a container 110 is mostly received in a recess 26 in the fully inserted configuration.
  • the peripheral wall 28 is configured to cover at least 50% of the peripheral surface 118 of a container 110 when said container 110 is fully inserted in the corresponding reception volume 26.
  • the peripheral wall covers at least 80% of the peripheral surface 118 of a fully inserted container 110.
  • the mounting base 20 comprises twelve reception volumes 26, arranged in an array of three rows 36 by four columns 34.
  • the number of reception volumes 26 is lower than twelve.
  • the number or reception volumes 26 is higher than twelve.
  • a mounting base with too many reception volumes 26 might be too wide and/or too heavy for an operator to handle while wearing gloves.
  • the number of reception volumes 26 is comprised between 1 and 100, preferably between 2 and 64, more preferably between 4 and 48, even more preferably equal to 12.
  • the number of reception volumes 26 is preferably equal to the product of two positive integers, so that the reception volumes 26 can be arranged in an array.
  • reception volumes 26 are arranged differently, for example in an array of two rows by six columns, or in a staggered arrangement, or in a straight line.
  • the container 110 comprises two inner partitions 124, which divide the inner volume 112 in four separated chambers 126.
  • the container 110 comprises only one inner partition 124, which divides the inner volume 112 in two separated chambers.
  • the container 110 comprises at least two inner partitions that extend radially from the main axis A110 and divide the inner volume 112 in at least two separated chambers.
  • the container 110 comprises three inner partitions, which divide the inner volume 112 in three separated chambers 126.
  • the number of chambers 126 the lower the ratio of unused volume within each vial 100. On the other hand, the higher the number of chambers 126, the smaller each chamber 126. Chambers 126 that are too small are not practical to retrieve the biological sample stored within a chamber 126. Generally, the number of separated chambers is comprised between 2 and 8, preferably between 3 and 6, still preferably equal to 4.
  • the number and location of the marks 128, distributed on the outer surface 116, and of the set of marks 38, arranged around each reception volume 26, are adjusted accordingly.
  • the bottom surface and the lateral surface of the plate 22 are also covered by a protection layer, which is made from a non-sticky material such as PTFE.
  • a protection layer which is made from a non-sticky material such as PTFE.
  • the plate 22 is encased in a protection layer, with each reception volume 26 extending through the protection layer 40 arranged on the top surface 24.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Medical Preparation Storing Or Oral Administration Devices (AREA)

Abstract

L'invention concerne un flacon (100) pour la cryoconservation de multiples échantillons biologiques. Ce flacon (100) comprend : un contenant (110), réalisé d'une seule pièce, définissant un volume interne (112) et une ouverture pourvue d'un filetage (132) ; et un capuchon fileté (150), qui coopère avec le filetage (132) de l'ouverture de façon à fermer sélectivement le volume interne (112), dans une configuration scellée du flacon (100), ou permettre l'accès au volume interne (112), dans une configuration non scellée du flacon (100). Selon l'invention, le contenant (110) comprend au moins une cloison interne (124), configurée pour diviser le volume interne (112) en au moins deux chambres séparées (126), chaque chambre (126) étant accessible depuis l'ouverture (114). Chaque chambre est configurée pour recevoir un échantillon biologique. Le flacon selon l'invention est facile à manipuler et permet de gagner de la place. L'invention concerne également une base de montage comprenant au moins un volume de réception évidé qui est configuré pour recevoir un tel flacon.
PCT/EP2022/066007 2021-06-14 2022-06-13 Flacon pour la cryoconservation d'échantillons biologiques multiples, base de montage associée et kit de cryoconservation comprenant un tel flacon et une telle base de montage, et procédé mis en œuvre avec un tel kit Ceased WO2022263364A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP21305811.8 2021-06-14
EP21305811 2021-06-14

Publications (1)

Publication Number Publication Date
WO2022263364A1 true WO2022263364A1 (fr) 2022-12-22

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PCT/EP2022/066007 Ceased WO2022263364A1 (fr) 2021-06-14 2022-06-13 Flacon pour la cryoconservation d'échantillons biologiques multiples, base de montage associée et kit de cryoconservation comprenant un tel flacon et une telle base de montage, et procédé mis en œuvre avec un tel kit

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WO (1) WO2022263364A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070104617A1 (en) 2005-11-04 2007-05-10 Advanced Biotechnologies Limited Capped tubes
WO2016178635A1 (fr) 2015-05-06 2016-11-10 National University Hospital (Singapore) Pte Ltd Flacons pour le stockage d'échantillons biologiques
CN206565163U (zh) 2017-03-15 2017-10-20 中国农业科学院兰州畜牧与兽药研究所 一种自带切割刀片的分隔式微量冻存管
US20190092555A1 (en) 2017-05-09 2019-03-28 Fibulas, Inc. Container for biological preservation at low temperature

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070104617A1 (en) 2005-11-04 2007-05-10 Advanced Biotechnologies Limited Capped tubes
WO2016178635A1 (fr) 2015-05-06 2016-11-10 National University Hospital (Singapore) Pte Ltd Flacons pour le stockage d'échantillons biologiques
CN206565163U (zh) 2017-03-15 2017-10-20 中国农业科学院兰州畜牧与兽药研究所 一种自带切割刀片的分隔式微量冻存管
US20190092555A1 (en) 2017-05-09 2019-03-28 Fibulas, Inc. Container for biological preservation at low temperature

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AMAZON: "Chemglass CLS-4774-01 Aluminum Cool Block", 6 December 2021 (2021-12-06), XP055869888, Retrieved from the Internet <URL:https://www.amazon.com/Chemglass-CLS-4774-01-Aluminum-Cryovials-Position/dp/B00DUG9L7Y> [retrieved on 20211206] *
CHEMGLASS: "CLS-4774 COOL BLOCKS, 32 POSITION, 1.5 AND 2.0ML CRYOVIALS", 9 July 2013 (2013-07-09), XP055869889, Retrieved from the Internet <URL:https://chemglass.com/cool-blocks-32-position-1-5-and-2-0ml-cryovials> [retrieved on 20211206] *

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