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WO2022159055A1 - Extrait de feuilles d'olivier et son procédé de production - Google Patents

Extrait de feuilles d'olivier et son procédé de production Download PDF

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Publication number
WO2022159055A1
WO2022159055A1 PCT/TR2021/050614 TR2021050614W WO2022159055A1 WO 2022159055 A1 WO2022159055 A1 WO 2022159055A1 TR 2021050614 W TR2021050614 W TR 2021050614W WO 2022159055 A1 WO2022159055 A1 WO 2022159055A1
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WIPO (PCT)
Prior art keywords
olive
leaf extract
olive leaf
production method
leaves
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Ceased
Application number
PCT/TR2021/050614
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English (en)
Inventor
İlhan Sari
Original Assignee
Medi̇cal Farm İlaç Ve Sağlik Ürünleri̇ Sanayi̇ Ve Ti̇caret Li̇mi̇ted Şi̇rketi̇
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Filing date
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Publication of WO2022159055A1 publication Critical patent/WO2022159055A1/fr
Anticipated expiration legal-status Critical
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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/08—Solutions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/4816—Wall or shell material

Definitions

  • the present invention relates to the aqueous total Olea europaea L. (olive) leaf extract which contains a highly valuable plant chemical substance effective against diabetes, cancer, cardiovascular, gastrointestinal diseases and covid-19 and loss of weight and its production method.
  • the present invention particularly relates to the aqueous total Olea europaea L. (olive) leaf extract which is developed for use within the scope of traditional and herbal medicinal products in the food supplement and pharmaceutical industry, contains a highly valuable plant chemical substance effective against diabetes, cancer, cardiovascular, gastrointestinal diseases and covid-19 and loss of weight and its production method.
  • aqueous total Olea europaea L. (olive) leaf extract which is developed for use within the scope of traditional and herbal medicinal products in the food supplement and pharmaceutical industry, contains a highly valuable plant chemical substance effective against diabetes, cancer, cardiovascular, gastrointestinal diseases and covid-19 and loss of weight and its production method.
  • the olive tree plant which is called Olea europaea L. in Latin, has been widely used against different diseases in phytotherapy, which is a sub-practice of traditional and alternative medicine, for many years.
  • it shall have drugs that are compatible with human metabolism with its ingredient, whose all valuable herbal chemical substances that have protective and/or therapeutic power against diseases are obtained after drying the leaves obtained by producing in the right geography with the correct agricultural production method with correct drying methods.
  • Olea europaea L. folium is defined as the dried leaf of the plant containing a minimum of 5% oleuropein (C25H32O13; Mr 540.5) (European Pharmacopoeia 01/2009: 1878).
  • Olea europaea L. leaf total extract is effective against diabetes, cancer, cardiovascular, gastrointestinal diseases and covid-19 due to its antiviral effect.
  • the phenolic and flavonoid composition of olive leaf extracts there are components such as gallic acid, chlorogenic acid, caffeic acid, ellagic acid, epicatechin, rutin, quercetin, querthrin and kaempferol.
  • the polyphenols and flavonoids obtained from olive leaves exhibit the abovementioned properties as herbal chemical substances, anti-carcinogen, anti-inflammatory, antihypertensive and antimicrobial. For this reason, these substances are necessary for the pharmacological effects of olive leaves.
  • the active component of olive leaf is oleuropein which is a secoiridoid reported to be a powerful antioxidant with anti-inflammatory properties.
  • Oleuropein has pharmacological and health improving properties including hypoglycemic, antioxidant, antimicrobial, antimycoplasmal, antiviral, anti-tumor and angiogenic activities. It has been found that it inhibits the activity of androstenedione 6p-hydroxylase, a cytochrome P450 3A marker, in human liver microsomes, prevents lipid peroxidation on rat liver microsomes. It has also been shown that oleuropein inhibits LDL (Low Density Lipoprotein) oxidation and has vascular protective activity by inhibiting platelet aggregation induced by platelet activating factor.
  • LDL Low Density Lipoprotein
  • iridoid monoterpenes Components of olive leaf are iridoid monoterpenes, triterpenes, flavonoids, kalkols, phenolic acids and coumarins.
  • iridoid monoterpenes among others oleuropein (5-9%), in addition 6-O-oleuropeine consists of sucrose, ligstroside, oleoroside and similar components.
  • Triterpenes consist of oleanolic acid, maslinic acid and similar components.
  • Flavonoids consist of luteolin, kaempferol, chrysoeriol and apigenin derivatives and similar components.
  • Kalkols consist of olivine, olivine-4'-0-diglucoside and similar components.
  • Phenolic acids consist of cumaric acid, caffeic acid, ferulic acid, vanillic acid and similar components.
  • Coumarins consist of aesculetin, scopoletin and aesculin components
  • the main components of olive leaves are oleuropein, ligstroside, I methyloleuropein and oleoside, and flavonoids (apigenin, kaempferol, luteolin, chrysoeriol) and phenolic compounds (caffeic acid, tyrosol, hydroxytrozole).
  • Secoiridoids are a very specific group found in plenty of in Oleaceas. Secoiridoids are produced from the secondary metabolism of terpene as precursors of various indole alkaloids in many other plants.
  • the oleoside-type glucoside is derived from oleosides. It is characterized by a combination of ellenolic acid and a glucosidic residue. These compounds can be considered to advance through the acetate/mevalonate pathway.
  • Oleuropein which is the main component of the secoiridoid family in olive trees, is a complex phenol found in high amounts in olive tree leaves and low in olive oil and has a bitter taste and strong aroma.
  • Olive leaves contain approximately 60-90 mg/g (dry weight) of oleuropein as well as significant levels of elenolic acid and hydroxytyrosol (3,4- dihydrophenylethanol) glucosidic ester.
  • oleuropein and its hydrolysis, oleuropein aglycon, elenolic acid, beta-3, 4-dihydroxyphenethyl alcohol and methyl-o- methyl enolate products emerge as the main molecules of biological interest.
  • olive leaf extract has been used against cardiovascular diseases in Germany since 1976 in the state of the art, in European drug agency records. Furthermore, olive leaf and its extracts are used in the complementary and alternative medicine community for their ability to act as the killer of natural pathogens by inhibiting the replication process of many pathogens. Olive leaf extract has also been used as a household remedy so as to battle against other illnesses such as fever and malaria. Furthermore, various reports have shown that olive leaf extract has the capacity to lower blood pressure, increase blood flow in coronary arteries, relieve arrhythmias, and prevent intestinal muscle spasms in animals. Olive leaf is also widely used to battle against viral infections such as cold and flu, yeast infections, and difficult to treat Epstein-Barr disease, shingles, and herpes etc. Furthermore, olive leaves are used to support the heart. It has been shown that olive leaf reduces low-density lipoproteins (LDL), lowers blood pressure and increases blood flow by relaxing the arteries.
  • LDL low-density lipoproteins
  • oleuropein makes the olive tree particularly durable and resistant to insect and bacterial damage.
  • Oleuropein is iridoid which is a structural class of chemical compounds found in plants. It is found in the olive tree, along the olive tree, and is a bitter material that is eliminated from the olives when they are dried.
  • An Italian researcher has reported that oleuropein lowers blood pressure in animals in 1962. This triggered scientific interest in the olive leaf.
  • Other European researchers confirm this interesting finding. Furthermore, it has been found that olive leaf can increase blood flow in coronary arteries, relieve arrhythmias, and prevent intestinal muscle spasms.
  • Dried or fresh leaves have also been used as an antipyretic and as a diuretic against malaria.
  • Today in addition to these, today, it is known to be effective against covid-19, which became a pandemic in December 2019 due to the antiviral, antimicrobial and historical use of olive tree leaf against malaria.
  • leaf and essential oil extracts obtained from olives are also used in anti-aging creams, hand creams, soaps and pediatric creams in the cosmetics sector.
  • the raw material source used in olive leaf extract which is observed to be effective against diabetes, cancer, cardiovascular, gastrointestinal diseases and covid-19 is obtained from the farm grown under the same conditions of two different types of olive trees, which were started to be cultivated in a certain geography and climate in Anatolia twenty years ago, without using chemical fertilizers, hormones or pesticides. It is important to standardize the agricultural cultivation method of the raw material so as to ensure that the products to be used in herbal treatment can always exhibit the same effect.
  • the present invention is related to an olive leaf extract and production method thereof which fulfills the abovementioned requirements, eliminates all disadvantages and brings some additional advantages.
  • the main aim of the invention is to develop an aqueous total olive leaf extract that contains multiple highly valuable plant chemical substances effective against diabetes, cancer, cardiovascular, gastrointestinal diseases and covid-19, in which the positive (synergistic) effect of all substances is observed together, in which this synergistic effect is obtained by revealing the protective or therapeutic properties of the diseases mentioned in human metabolism.
  • the aim of the invention is to develop an olive leaf extract which is a supporting or alternative treatment for diabetes, cancer, cardiovascular, gastrointestinal diseases and covid-19 patients, reduces undesired side effects, increases the quality of life by preventing the formation of other diseases that occur as a side effect after covid-19 treatment, which has been exposed to these diseases and has recently turned into a pandemic.
  • a similar aim of the invention is to develop an aqueous total olive leaf extract whose effectiveness is increased against diabetes, cancer, cardiovascular, gastrointestinal diseases and COVID-19 with a synergistic effect between them by not isolating the active ingredients.
  • Another aim of the invention is to develop an aqueous total olive leaf extract containing more than 2000 active ingredients in their own proportions, which are synthesized during the life of the plant so as to be more effective against said diseases.
  • Another aim of the invention is to develop an olive leaf extract with reduced production cost.
  • Another aim of the invention is to develop an olive leaf extract that does not contain any of the preservatives or fillers added in most food supplements and traditional herbal products, thus that does not lead to the adverse effects caused by these substances.
  • Another aim of the invention is to develop a total aqueous extract of the olive leaf in the form of liquid extract that provides easy use for children, the elderly or people who have difficulty swallowing products produced in pellet form.
  • the inventive olive oil extract is a total aqueous extract of Olea europaea L. (olive) leaf, which contains a high percentage of valuable vegetable chemicals
  • the inventive method comprises the following process steps; minimizing olive leaves dried with a standardized method, mixing and keeping the leaves in the presence of water at maximum surface film forming capacity and removing the solvent from the obtained filtrate under vacuum.
  • inventive olive leaf extract and the production method thereof is described only for clarifying the subject matter in a manner such that no limiting effect is created.
  • the subject of the present invention is the total aqueous Olea europaea L. (olive) leaf extract which contains a highly valuable plant chemical substance effective against diabetes, cancer, and cardiovascular, gastrointestinal diseases and covid-19. It’s being total is thought to be effective against diabetes, cancer, cardiovascular, gastrointestinal diseases and covid-19 with its high synergistic effect in terms of approximately 2000 active ingredients in the olive leaf content.
  • the positive (synergistic) effect of all the substances it contains is observed. This synergistic effect has protective or therapeutic properties in said diseases in human metabolism.
  • the inventive olive leaf extract is a natural plant extract.
  • the inventive production method invention aims to reveal the synergistic effect of the total extract and herbal chemicals and simultaneously to increase its applicability in the industry by reducing the production cost.
  • the olive leaves which have been first dried by a standardized method, are preferably reduced to the range of 0.2-4 cm so as to achieve this. Subsequently, olive leaves are extracted in a dark environment. Then, the leaves are preferably kept by stirring at a temperature of 18-100 °C at a rotation speed of 10-60 rpm in the range of 5-72 hours in the presence of water at maximum surface film forming capacity.
  • the solvent is removed from the obtained filtrate, preferably in the range of 1-10 hours, at a rotational speed of 10-70 rpm at a temperature of 18-100 ° C and a vacuum of 2-100 mbar. It is ensured that the active substances pass to the solvent in a certain time without deterioration at the same rate after all these steps are carried out.
  • the aqueous total olive leaf extract obtained in this manner is much more appropriate in terms of production costs.
  • the active ingredients have not been isolated and have increased their effectiveness against diabetes, cancer, cardiovascular, gastrointestinal diseases and covid-19 with a synergistic effect between each other in olive leaf extract obtained by this production method.
  • the inventive olive leaf extract can be used within the scope of food supplement in the food industry against diabetes, cancer, cardiovascular, gastrointestinal diseases and COVID-19 and as and traditional and herbal medicinal products in the pharmaceutical industry.
  • Application of the invention may be encapsulated into a liquid drop or a slow release soft gel capsule in the stomach or intestine. Since there is a form in the application, it provides easy use for children, the elderly or people who have difficulty swallowing products produced in pellet form. This liquid form can be used orally in a practical manner.

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

La présente invention concerne en particulier l'extrait total aqueux de feuilles (d'olivier) Olea europaea L. qui est développé pour être utilisé dans le cadre de produits de phytothérapie classiques dans l'industrie pharmaceutique et des compléments alimentaires, et qui contient une substance chimique végétale extrêmement bénéfique efficace contre le diabète, le cancer, les maladies cardiovasculaires, les maladies gastro-intestinales, la covid-19 et la perte de poids, et son procédé de production. À cette fin, l'extrait de feuilles d'olivier selon l'invention contient des principes actifs et de l'eau que la plante a synthétisés lors de sa durée de vie, et le procédé selon l'invention comprend les étapes de procédé suivantes consistant à : réduire au minimum les feuilles d'olivier séchées au moyen d'un procédé normalisé, mélanger et conserver les feuilles dans de l'eau à une capacité de formation de film de surface maximale, et éliminer le solvant du filtrat obtenu sous vide.
PCT/TR2021/050614 2021-01-25 2021-06-16 Extrait de feuilles d'olivier et son procédé de production Ceased WO2022159055A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR2021/01093 2021-01-25
TR2021/01093A TR202101093A1 (tr) 2021-01-25 2021-01-25 Bir zeytin yaprağı ekstresi ve bunun üretim yöntemi

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Publication Number Publication Date
WO2022159055A1 true WO2022159055A1 (fr) 2022-07-28

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TR (1) TR202101093A1 (fr)
WO (1) WO2022159055A1 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109043261A (zh) * 2018-08-20 2018-12-21 德昌泓源农林科技发展有限公司 一种饮料制备工艺

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109043261A (zh) * 2018-08-20 2018-12-21 德昌泓源农林科技发展有限公司 一种饮料制备工艺

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GUMUSBULUT, G.: "Extraction of oleuropein from olive leaves (Master's thesis, Izmir Institute of Technology", EXTRACTION OF OLIVE LEAVES, pages 1 - 20, XP055957982 *
HAKIM BENDI: "Nature's Plus Herbal Actives Tri-Immune 60 Extended Release Tablets - ", ZEN HEALTHCARE, 8 July 2017 (2017-07-08), XP055958009, Retrieved from the Internet <URL:https://www.zenhealthcare.co.uk/product/natures-plus-herbal-actives-tri-immune-60-extended-release-tablets/> [retrieved on 20220906] *
MONTELEONE JULIETA INES, SPERLINGA ELISA, SIRACUSA LAURA, SPAGNA GIOVANNI, PARAFATI LUCIA, TODARO ALDO, PALMERI ROSA: "Water as a Solvent of Election for Obtaining Oleuropein-Rich Extracts from Olive (Olea europaea) Leaves", AGRONOMY, vol. 11, no. 3, 3 March 2021 (2021-03-03), pages 465, XP055958041, DOI: 10.3390/agronomy11030465 *
SCHLEICHER ANNI: "Antiviral effects of olive leaf extracts flagged amid heightened immunity tensions", NUTRITIONINSIGHT, 31 March 2020 (2020-03-31), pages 1 - 2, XP055958019, Retrieved from the Internet <URL:https://www.nutritioninsight.com/news/antiviral-effects-of-olive-leaf-extracts-flagged-amid-heightened-immunity-tensions.html> [retrieved on 20220906] *

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