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WO2022035026A1 - Composition cosmétique antioxydante, anti-inflammatoire ou améliorant la peau comprenant un extrait de propolis fermenté en tant que principe actif - Google Patents

Composition cosmétique antioxydante, anti-inflammatoire ou améliorant la peau comprenant un extrait de propolis fermenté en tant que principe actif Download PDF

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Publication number
WO2022035026A1
WO2022035026A1 PCT/KR2021/006560 KR2021006560W WO2022035026A1 WO 2022035026 A1 WO2022035026 A1 WO 2022035026A1 KR 2021006560 W KR2021006560 W KR 2021006560W WO 2022035026 A1 WO2022035026 A1 WO 2022035026A1
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Prior art keywords
propolis
fermented
cosmetic composition
inflammatory
skin
Prior art date
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Ceased
Application number
PCT/KR2021/006560
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English (en)
Korean (ko)
Inventor
이소정
한상근
최형
이승재
박정미
이상용
배연수
김재민
윤재웅
배해병
김현수
안민정
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Kolmar Korea Co Ltd
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Kolmar Korea Co Ltd
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Publication of WO2022035026A1 publication Critical patent/WO2022035026A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
    • A61K8/988—Honey; Royal jelly, Propolis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Definitions

  • the present invention relates to a cosmetic composition for antioxidant, anti-inflammatory or skin improvement comprising a propolis fermented extract as an active ingredient.
  • Propolis is a substance made by bees by mixing their own saliva and enzymes with resin-like substances extracted from various plants for their survival and reproduction. It is also called Russian penicillin or natural penicillin. There are many differences in the composition and content of propolis depending on which plant the bees use to make it. In particular, since the types of plants and climate conditions are different for each country, there are many differences depending on the collection area. .
  • propolis contains a large amount of active ingredients such as flavonoids, amino acids, and minerals, and thus can implement effects such as antioxidant, anti-inflammatory, immune activity, antibacterial, and anticancer. Accordingly, as disclosed in Korean Patent Registration No. 10-1247375, it is mainly used as a material for foods and health foods that activate the functions of the human body through ingestion.
  • a mixed extract of hydrolytic enzyme-treated propolis, royal jelly and honey and an enzyme extract of kava kava, turmeric and cacao seeds are used as active ingredients, 0.1 to 10 based on the total weight of the cosmetic composition.
  • a cosmetic composition containing % by weight.
  • Republic of Korea Patent No. 10-1835799 discloses a first preparation containing propolis extract, glycerin, butylene glycol, etc., propolis extract, adenosine, beta-glucan, hutgae tree extract, green tea extract, black bean extract, etc.
  • a cosmetic composition in which a second agent containing
  • the present inventors have completed the present invention by developing a fermented propolis extract with significantly improved antioxidant, anti-inflammatory or skin improvement effects by constructing a plurality of propolis with different characteristics, fermenting and extracting them. did
  • Patent Document 1 Republic of Korea Patent No. 10-1247375
  • Patent Document 2 Republic of Korea Patent No. 10-1884660
  • Patent Document 3 Republic of Korea Patent No. 10-1835799
  • the first object of the present invention is to provide a cosmetic composition that is remarkably excellent in antioxidant, anti-inflammatory or skin improvement effects by including a fermented propolis extract obtained by mixing and fermenting different types of propolis.
  • a second object of the present invention is to provide a method for preparing a cosmetic composition having excellent antioxidant, anti-inflammatory or skin improvement effects, including the fermented propolis extract.
  • the object of the present invention is not limited to the technical problem as described above, and another technical problem may be derived from the following description.
  • the present invention fermented and extracted a propolis complex containing gold propolis, green propolis and red propolis, and contains a propolis fermented extract as an active ingredient, antioxidant, anti-inflammatory or skin
  • a cosmetic composition for improvement is provided.
  • the propolis complex may include the gold propolis, green propolis and red propolis in a weight ratio of 1: 0.1-5: 0.1-5.
  • the fermentation may be fermenting the propolis complex into a Lactobacillus strain.
  • the Lactobacillus strain may include Lactobacillus plantarum .
  • the skin improvement may be skin moisturizing or skin softness improvement.
  • the cosmetic composition may include 0.01 to 30% by weight of the fermented propolis extract based on the total weight.
  • the cosmetic composition is from the group consisting of softening lotion, nourishing lotion, astringent lotion, skin, lotion, essence, serum, cream, massage cream, pack, makeup base, BB cream, foundation, powder, cleansing foam, cleansing cream and cleansing water. It may be one selected formulation.
  • the present invention provides a preparation step of preparing a propolis complex comprising gold propolis, green propolis and red propolis; A primary fermentation step of aging the propolis complex in a jar; a secondary fermentation step of inoculating the first fermented fermented product with a strain and fermenting it; and an extraction step of extracting the second fermented fermented product to form a propolis fermented extract; A manufacturing method is provided.
  • the cosmetic composition of the present invention contains, as an active ingredient, a fermented propolis extract obtained by fermenting and extracting a propolis complex containing gold propolis, green propolis and red propolis, thereby exhibiting remarkably excellent antioxidant, anti-inflammatory or skin improvement effects.
  • a propolis complex comprising gold propolis, green propolis and red propolis is double fermented according to two steps to obtain a fermented propolis extract, resulting in significantly excellent antioxidant, It is possible to prepare a cosmetic composition that implements an anti-inflammatory or skin improvement effect.
  • a and/or B means A or B, or A and B.
  • the present invention provides a cosmetic composition for antioxidant, anti-inflammatory or skin improvement comprising a propolis fermented extract as an active ingredient.
  • the fermented propolis extract obtained by fermenting and extracting a propolis complex containing gold propolis, green propolis and red propolis as an active ingredient significantly excellent antioxidant, anti-inflammatory or skin improvement effect It provides a cosmetic composition having.
  • a propolis complex containing gold propolis, green propolis and red propolis is fermented and extracted, and a propolis fermented extract with a high content of active ingredients such as polyphenol and artepillin C
  • active ingredients such as polyphenol and artepillin C
  • Implementing the skin improvement effect may mean implementing the skin moisturizing improvement effect and skin softness improvement effect, but is not limited thereto.
  • the fermented propolis extract of this embodiment and the cosmetic composition comprising the same have antioxidant and anti-inflammatory effects, thereby restoring the function of the skin dermis, thereby improving skin moisture, skin complexion, and skin softness.
  • the propolis complex includes gold propolis, green propolis and red propolis.
  • Gold propolis is also called brown propolis or yellow propolis, and refers to propolis having a color of gold, brown or yellow.
  • the gold propolis is produced in various countries such as Brazil, New Zealand, North America, and China. Specifically, the gold propolis of this embodiment may be from Australia, but is not limited thereto.
  • Green propolis is mainly manufactured using the resin of the Baccharis plant, and it is a green propolis that contains a high content of flavonoids and Artepillin C, By itself, it can have antibacterial, anticancer, and antioxidant effects.
  • the green propolis of this embodiment may be from Brazil, but is not limited thereto.
  • Red propolis is a red propolis, and it can mean that it is made with the red sap of a tree that grows only in the clean areas of northeast Brazil.
  • red propolis has a higher polyphenol content than gold propolis and green propolis, and contains isoflavones, an antioxidant component, so it has effects such as antioxidant and skin immunity enhancement.
  • the red propolis of this embodiment may be from Brazil, but is not limited thereto.
  • the fermented propolis extract of this embodiment is polyphenol, artephyllin It may contain active ingredients such as C in a high content. Accordingly, the cosmetic composition of this embodiment can implement excellent antioxidant, anti-inflammatory or skin improvement effects.
  • the propolis complex of this embodiment includes the three types of propolis described above, and may include the gold propolis, green propolis and red propolis in a weight ratio of 1: 0.1 to 5: 0.1 to 5.
  • the propolis complex of this embodiment contains the gold propolis, green propolis and red propolis in a weight ratio of 1: 0.1 to 5: 0.1 to 5
  • the fermented propolis extract of this embodiment and its containing The antioxidant, anti-inflammatory or skin improvement effect of the cosmetic composition may be improved.
  • the propolis complex of this embodiment may include the gold propolis, green propolis and red propolis in a weight ratio of 1: 2 to 4: 1.
  • the propolis complex of this embodiment contains the gold propolis, green propolis and red propolis in a weight ratio of 1: 2 to 4: 1, the fermented propolis extract of this embodiment and a cosmetic comprising the same
  • the antioxidant, anti-inflammatory or skin improving effect of the composition may be further enhanced.
  • the above effects can be further improved.
  • the propolis complex of this embodiment may include the gold propolis, green propolis and red propolis in a weight ratio of 1: 4: 1. According to the above, when the propolis complex of this embodiment contains the gold propolis, green propolis and red propolis in a weight ratio of 1: 4: 1, the fermented propolis extract of this embodiment and the cosmetic composition comprising the same Antioxidant, anti-inflammatory or skin-improving effects can be significantly improved.
  • the fermented propolis extract is fermented and extracted from the propolis complex.
  • the fermented propolis extract of this embodiment may be obtained by fermenting and then extracting the propolis complex.
  • the fermentation may be carried out by inoculating and fermenting a strain applicable to the fermentation of the propolis complex, but is not limited thereto.
  • the fermentation may be carried out by secondary fermentation in which the propolis complex is put in a jar and aged for primary fermentation, and then the primary fermented fermented product is inoculated with a strain applicable to fermentation and fermented.
  • the fermented propolis extract of this embodiment can contain a higher content of active ingredients such as polyphenol and artephyllin C by double fermenting a propolis complex in which three types of propolis are mixed. Accordingly, the antioxidant, anti-inflammatory or skin improvement effect of the cosmetic composition comprising the fermented propolis extract can be significantly improved.
  • the strain applicable to the fermentation may be a Lactobacillus strain, but is not limited thereto.
  • the strain applicable to the fermentation may include, but is not limited to, Lactobacillus plantarum .
  • the strain applicable to the fermentation may include Lactobacillus plantarum KCC-32 (Accession No.: KACC92135P), but is not limited thereto.
  • the strain includes Lactobacillus plantarum KCC-32 (accession number: KACC92135P), polyphenols, artepilin C, etc.
  • a police fermentation extract may be provided. Accordingly, the antioxidant, anti-inflammatory or skin improvement effect of the cosmetic composition comprising the fermented propolis extract can be significantly improved.
  • the extraction may be performed by any known extraction method, for example, the extraction is one or more extraction methods selected from the group consisting of hot water extraction method, room temperature stirring extraction method, reflux cooling extraction method, ultrasonic extraction method, high temperature pressure extraction method, and low temperature high pressure extraction method It may be carried out by, but is not limited to.
  • the cosmetic composition of this embodiment may contain 0.01 to 30% by weight of the fermented propolis extract, specifically 0.1 to 20% by weight, and more specifically 1 to 10% by weight based on the total weight of the cosmetic composition.
  • the cosmetic composition of this embodiment contains 0.01 to 30% by weight of the fermented propolis extract based on the total weight, it is possible to provide a cosmetic composition having an excellent antioxidant, anti-inflammatory or skin improvement effect while having a stable formulation. .
  • the cosmetic composition of this embodiment consists of softening lotion, nourishing lotion, astringent lotion, skin, lotion, essence, serum, cream, massage cream, pack, makeup base, BB cream, foundation, powder, cleansing foam, cleansing cream and cleansing water. It may be one formulation selected from the group, but is not limited thereto, and may be implemented as a formulation of all known cosmetic compositions.
  • the cosmetic composition of this embodiment may include excipients or additives necessary for the formulation of each formulation in addition to the fermented propolis extract.
  • the excipients or additives include surfactants, emulsifiers, emulsifying aids, silicone polymers, pigments, pigments, fragrances, preservatives, bactericides, oxidation stabilizers, organic solvents, thickeners, emollients, antioxidants, free radical scavengers, opacity Topical, stabilizer, emollient, silicone, antifoam, humectant, vitamin, insect repellent, preservative, anti-inflammatory, filler, polymer, propellant, basifying agent, acidifying agent, colorant, thickening agent, skin conditioning agent, whitening agent, antioxidant, anti-wrinkle agent and the like, but is not limited thereto.
  • the present invention provides a method for preparing a cosmetic composition for antioxidant, anti-inflammatory or skin improvement comprising a fermented propolis extract as an active ingredient.
  • a preparation step of preparing a propolis complex comprising gold propolis, green propolis and red propolis; A primary fermentation step of aging the propolis complex in a jar; a secondary fermentation step of inoculating the first fermented fermented product with a strain and fermenting it; and an extraction step of extracting the second fermented fermented product to form a propolis fermented extract do.
  • a propolis complex containing gold propolis, green propolis and red propolis is fermented and extracted, and a propolis fermented extract with a high content of active ingredients such as polyphenol and artepillin C is used.
  • active ingredients such as polyphenol and artepillin C
  • a propolis complex including gold propolis, green propolis and red propolis is prepared.
  • the preparation step of this embodiment may be carried out at room temperature conditions, but is not limited thereto.
  • a propolis complex can be prepared by mixing and pre-treating propolis including gold propolis, green propolis and red propolis in a resin state.
  • gold propolis, green propolis and red propolis in a resin state are mixed, immersed in an organic solvent to separate a lower layer and an upper layer containing wax, and the upper layer is filtered and By concentrating under reduced pressure and lyophilizing, the propolis complex in powder form can be prepared.
  • the organic solvent may include any known organic solvent, for example, may include ethanol, but is not limited thereto.
  • the primary fermentation step of this embodiment the primary fermentation is carried out in which the propolis complex prepared according to the above preparation step is put in a jar and aged.
  • the primary fermentation step of this embodiment may be carried out at room temperature conditions, but is not limited thereto.
  • the propolis complex is mixed with an organic solvent and water, and then put in a jar and aged for a certain period of time, thereby performing primary fermentation.
  • the organic solvent may include all known organic solvents, for example, 1,3-butylene glycol, but is not limited thereto.
  • the primary fermentation of this embodiment may be carried out for 5 to 10 days, specifically 6 to 8 days. If, when the primary fermentation is carried out for less than 5 days or more than 10 days, the antioxidant of the fermented propolis extract and cosmetic composition comprising the same than when the primary fermentation is carried out for 5 to 10 days , the anti-inflammatory or skin improvement effect may be insignificant.
  • the secondary fermentation is performed by inoculating the fermented product that is first fermented according to the first fermentation step with a strain applicable to fermentation.
  • the secondary fermentation may be performed by inoculating the Lactobacillus strain into the primary fermented ferment.
  • the fermented propolis extract prepared according to this embodiment contains an active ingredient in a high content, and its antioxidant, anti-inflammatory or skin improvement effect can be excellent.
  • the secondary fermentation may be performed by inoculating the first fermented fermented product with Lactobacillus plantarum .
  • the fermented propolis extract prepared according to this example contains an active ingredient in a high content, and its antioxidant, anti-inflammatory or skin improvement effect is more can be excellent
  • the secondary fermentation may be performed by inoculating the fermented product with Lactobacillus plantarum KCC-32 (Accession No.: KACC92135P).
  • Lactobacillus plantarum KCC-32 accesion No.: KACC92135P
  • the content of active ingredients in the fermented propolis extract prepared according to this example is significantly increased, and its antioxidant, anti-inflammatory, or skin The improvement effect may be remarkably excellent.
  • the secondary fermentation of this embodiment may be carried out at a temperature of 28 to 40 °C, specifically 30 to 35 °C, but is not limited thereto. If the secondary fermentation of this embodiment is carried out at less than 28 °C or is carried out at more than 40 °C, than when the secondary fermentation is carried out at 28 to 40 °C, the activity of microorganisms is inhibited, and the propolis fermentation extract and The improvement of the antioxidant, anti-inflammatory, or skin improvement effect of the cosmetic composition comprising the same may be insignificant.
  • the secondary fermentation of this example may be carried out in a pH condition of pH 5.5 to 6.8, specifically pH 6 to 6.5, but is not limited thereto. If the secondary fermentation of this example is carried out at less than pH 5.5 or at more than pH 6.8, the activity of microorganisms is inhibited, compared to the case of pH 5.5 to 6.8, and the fermentation extract of propolis and the cosmetic composition comprising the same The improvement in antioxidant, anti-inflammatory or skin-improving effects may be negligible.
  • the secondary fermentation of this embodiment may be carried out for 5 to 10 days, specifically 6 to 8 days. If, when the secondary fermentation is carried out for less than 5 days or more than 10 days, the antioxidant of the fermented propolis extract and cosmetic composition comprising the same than when the secondary fermentation is carried out for 5 to 10 days , the anti-inflammatory or skin improvement effect may be insignificant.
  • a fermented propolis extract is formed by extracting the fermented product secondarily fermented according to the second fermentation step.
  • the fermented product secondarily fermented according to the second fermentation step is selected from the group consisting of hot water extraction method, room temperature stirring extraction method, reflux cooling extraction method, ultrasonic extraction method, high temperature pressure extraction method, and low temperature and high pressure extraction method
  • the liquid component is filtered and removed to form a propolis fermentation extract.
  • a propolis fermentation extract can be formed by extracting the second fermented product by ultrasonic extraction according to the second fermentation step, and then filtering and removing the liquid component, but limited thereto doesn't happen
  • the ultrasonic extraction may be performed at a temperature of 15 to 20 °C and a frequency of 20 to 40 kHz, but is not limited thereto.
  • the propolis fermentation extract obtained according to the above may be further subjected to a known post-treatment, but is not limited thereto.
  • the post-treatment may include, but is not limited to, freeze-drying, high-temperature drying, pulverization, and the like.
  • the cosmetic composition can be prepared by mixing the excipients or additives necessary for formulation of the formulation with the fermented propolis extract obtained according to the above.
  • the excipients or additives include surfactants, emulsifiers, emulsifying aids, silicone polymers, pigments, pigments, fragrances, preservatives, bactericides, oxidation stabilizers, organic solvents, thickeners, emollients, antioxidants, free radical scavengers, opacity Topical, stabilizer, emollient, silicone, antifoam, humectant, vitamin, insect repellent, preservative, anti-inflammatory, filler, polymer, propellant, basifying agent, acidifying agent, colorant, thickening agent, skin conditioning agent, whitening agent, antioxidant, anti-wrinkle agent and the like, but is not limited thereto.
  • the manufacturing method of the cosmetic composition of this embodiment includes all of the above 'cosmetic composition for antioxidation, anti-inflammatory or skin improvement comprising fermented propolis extract as an active ingredient'.
  • the propolis complex of Preparation Example 1 prepared according to the above was placed in each jar and aged for 7 days, while primary fermentation was performed. At this time, a mixture of the propolis complex, an organic solvent and water was used, and based on the total amount, 1 wt% of the propolis complex (powder), 10 wt% of 1,3-butylene glycol, and 89 wt% of water were used. It was mixed and fermented.
  • Lactobacillus plantarum used for the secondary fermentation was the following, and 0.5 to 1% of Lactobacillus plantarum for the primary fermentation (concentration: 2 ⁇ 10 5 cfu/ml) was inoculated with
  • the propolis fermentation extract of Example 1 was finally obtained.
  • the ultrasonic extraction was carried out for 2 hours at a temperature of 15 to 20 °C under a frequency condition of about 20 to 40 kHz.
  • the filtration was performed by ultrasonically extracting each of the secondary fermented products, and then filtering them with GFC (1-3 ⁇ m filter paper), 0.8 ⁇ m filter paper, and 0.2 ⁇ m filter paper in this order.
  • the deposit institutions of Lactobacillus plantarum KCC-32 (accession number: KACC92135P) used for the fermentation are as follows.
  • DPPH radical scavenging ability (1-absorbance of the unadded group / absorbance of the untreated group) * 100
  • Tannic acid (sigma chemical co.) was used as a standard material.
  • the sample was analyzed with high performance liquid chromatography (HPLC, YL9100 HPLC system; Younglin Instruments CO., Ltd., Gyeonggi, Korea) to measure the content of artephyllin C in the sample, and the results are shown in Table 5 [unit: ppm] shown in At this time, artepylline C was detected at a retention time of 37 minutes.
  • HPLC high performance liquid chromatography
  • HPLC analysis conditions are as follows.
  • Nitric oxide (NO) content was measured by the amount of nitric oxide (NO) produced in the supernatant of the cell.
  • the nitrite reduced with nitrate was used as a griess reagent (Sigam, USA), and 2 X 10 6 cells in a 6 well plate were washed twice with PBS when the confluence was 80%, and then serum-free medium was used. Incubated for more than 12 hours, and reacted by treatment with LPS (lipopolysaccharide) 10 ⁇ g / ml. Then, after about 2 hours, a sample (treatment concentration: 1%) was treated and tested.
  • LPS lipopolysaccharide
  • fibroblasts In a 96 well plate, 1 X 10 4 cells/well of fibroblasts (Fibroblast HS86) were cultured for 24 hours. Thereafter, it was replaced with a serum-free medium and treated with 100 mM H 2 O 2 for 30 minutes, and then the samples were treated for each concentration and cultured for about 24 hours.
  • dexamethasone was treated at concentrations of 0.1%, 0.5% and 1.0%.
  • concentration (%) of TNF- ⁇ in the culture solution treated with the sample (fermented propolis extract) was analyzed compared to the untreated comparative culture solution.
  • the protective effect on the cells of the sample by oxidation was analyzed by applying the MTT assay method.
  • Example 17 when comparing Example 17 with Comparative Examples 4 to 6, it can be seen that the cytoprotective effect is improved when the three types of propolis are mixed and used even though the total amount of the total propolis is the same.
  • the cosmetic composition (cream formulation) prepared according to the above was applied directly to the skin, and the degree of skin condition improvement was evaluated according to the following, and the results are shown in Tables 12 [unit: score] and 13.
  • each group (composed of 20 women) was applied immediately after face-washing, twice a day, for about 2 weeks, and the skin moisture improvement effect was evaluated on a scale of 1 to 5, and the average value is shown in Table 12. It was In this case, the closer to 5 points, the better the skin moisturizing effect.
  • the skin softness improvement effect was evaluated on a scale of 1 to 5 in the same manner as above, and the average value thereof is shown in Table 13. In this case, the closer to 5 points, the better the skin softness improvement effect.
  • the skin moisturizing improvement effect and the skin softness improvement effect are excellent. That is, according to the present embodiment, it means that the skin condition is improved by providing moisture to the skin and softening the skin surface.
  • the fermented propolis extract according to this example has excellent DPPH radical scavenging ability and has an antioxidant effect as it contains polyphenol and artephyllin C in high content.
  • the fermented propolis extract according to this example has an anti-inflammatory effect while suppressing NO production and TNF- ⁇ activity and containing polyphenols and artefylline C in high content.
  • the fermented propolis extract according to this example has excellent DPPH radical scavenging ability, NO production inhibitory ability and TNF- ⁇ activity inhibitory ability, and contains active ingredients such as polyphenol and artephyllin C in a high content, thereby improving skin condition it can be seen that
  • the fermented propolis extract according to this embodiment has excellent antioxidant, anti-inflammatory and skin improvement effects without cellular damage.

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Abstract

La présente invention concerne : une composition cosmétique qui comprend, en tant que principe actif, un extrait de propolis fermenté obtenu par fermentation et extraction à partir d'un complexe de propolis comprenant de la propolis d'or, de la propolis verte et de la propolis rouge et peut ainsi produire un effet antioxydant, anti-inflammatoire ou améliorant la peau nettement supérieur ; et un procédé de production de la composition cosmétique.
PCT/KR2021/006560 2020-08-12 2021-05-26 Composition cosmétique antioxydante, anti-inflammatoire ou améliorant la peau comprenant un extrait de propolis fermenté en tant que principe actif Ceased WO2022035026A1 (fr)

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KR1020200101267A KR102454297B1 (ko) 2020-08-12 2020-08-12 프로폴리스 발효추출물을 유효성분으로 포함하는 항산화, 항염증 또는 피부 개선용 화장료 조성물

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008011806A (ja) * 2006-07-07 2008-01-24 Api Co Ltd プロポリス組成物及びその健康食品
KR20160148174A (ko) * 2015-06-16 2016-12-26 주식회사 아미코스메틱 프로폴리스 복합 추출물을 함유하는 화장료 조성물
KR101719223B1 (ko) * 2014-10-28 2017-03-23 주식회사 코리아나화장품 김치유산균으로 발효된 인삼, 꿀 및 프로폴리스의 발효액을 함유한 세라마이드 생합성 촉진용 화장료 조성물
KR20170141564A (ko) * 2016-06-15 2017-12-26 목포대학교산학협력단 프로폴리스 및 천년초 추출물을 유효성분으로 포함하는 화장료 조성물
KR101884660B1 (ko) * 2018-05-18 2018-08-29 한국콜마주식회사 프로폴리스, 로얄젤리 및 꿀을 포함하는 천연물의 효소추출물을 함유하는 화장료 조성물
KR20190037926A (ko) * 2017-09-29 2019-04-08 서울프로폴리스 주식회사 원산지가 다른 프로폴리스 추출물을 혼합 사용하는 프로폴리스의 사용방법 및 프로폴리스를 유효성분으로 하는 건강기능식품용 조성물
KR102135551B1 (ko) * 2019-08-29 2020-07-20 주식회사 씨앤피코스메틱스 포름오노네틴을 지표성분으로 포함하는 레드 프로폴리스 추출물을 포함하는 안티에이징 효과가 우수한 화장료 조성물

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100025035A (ko) * 2008-08-27 2010-03-09 박일환 프로폴리스 발효물의 제조방법
KR101247375B1 (ko) 2012-07-18 2013-04-02 박상헌 아사이베리와 프로폴리스를 이용한 건강식품의 제조방법
KR101835799B1 (ko) 2016-04-11 2018-03-07 (주)라파프로폴리스 프로폴리스를 유효성분으로 함유하는 화장품 조성물 및 상기 조성물을 포함하는 화장품
KR101894175B1 (ko) * 2017-01-17 2018-08-31 대전대학교 산학협력단 프로폴리스, 모링가 및 상엽 혼합 추출물을 유효성분으로 함유하는 화장료 조성물

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008011806A (ja) * 2006-07-07 2008-01-24 Api Co Ltd プロポリス組成物及びその健康食品
KR101719223B1 (ko) * 2014-10-28 2017-03-23 주식회사 코리아나화장품 김치유산균으로 발효된 인삼, 꿀 및 프로폴리스의 발효액을 함유한 세라마이드 생합성 촉진용 화장료 조성물
KR20160148174A (ko) * 2015-06-16 2016-12-26 주식회사 아미코스메틱 프로폴리스 복합 추출물을 함유하는 화장료 조성물
KR20170141564A (ko) * 2016-06-15 2017-12-26 목포대학교산학협력단 프로폴리스 및 천년초 추출물을 유효성분으로 포함하는 화장료 조성물
KR20190037926A (ko) * 2017-09-29 2019-04-08 서울프로폴리스 주식회사 원산지가 다른 프로폴리스 추출물을 혼합 사용하는 프로폴리스의 사용방법 및 프로폴리스를 유효성분으로 하는 건강기능식품용 조성물
KR101884660B1 (ko) * 2018-05-18 2018-08-29 한국콜마주식회사 프로폴리스, 로얄젤리 및 꿀을 포함하는 천연물의 효소추출물을 함유하는 화장료 조성물
KR102135551B1 (ko) * 2019-08-29 2020-07-20 주식회사 씨앤피코스메틱스 포름오노네틴을 지표성분으로 포함하는 레드 프로폴리스 추출물을 포함하는 안티에이징 효과가 우수한 화장료 조성물

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