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WO2022012562A1 - Pipette, appareil de traitement d'échantillon, et système de diagnostic moléculaire - Google Patents

Pipette, appareil de traitement d'échantillon, et système de diagnostic moléculaire Download PDF

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Publication number
WO2022012562A1
WO2022012562A1 PCT/CN2021/106145 CN2021106145W WO2022012562A1 WO 2022012562 A1 WO2022012562 A1 WO 2022012562A1 CN 2021106145 W CN2021106145 W CN 2021106145W WO 2022012562 A1 WO2022012562 A1 WO 2022012562A1
Authority
WO
WIPO (PCT)
Prior art keywords
valve body
sealing member
valve
pipetting
groove
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2021/106145
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English (en)
Chinese (zh)
Inventor
黄宏坤
张涛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangdong Runpon Bioscience Co Ltd
Original Assignee
Guangdong Runpon Bioscience Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangdong Runpon Bioscience Co Ltd filed Critical Guangdong Runpon Bioscience Co Ltd
Publication of WO2022012562A1 publication Critical patent/WO2022012562A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Definitions

  • the present disclosure relates to the field of microfluidic control for in vitro diagnosis, and in particular, to a pipetting valve, a sample processing device and a molecular diagnosis system.
  • pipetting valves are often used to transfer samples or reagents.
  • Existing pipetting valves usually include two valve bodies, one valve body forms multiple flow channels respectively communicating with multiple reagent chambers, and the other valve body is movably connected therein for reagent transfer;
  • For the seal between the two valve bodies of the liquid valve one is to use end face sealing, which requires high plane accuracy of the sealing end faces of the two valve bodies and is difficult to process;
  • the sealing surface is encapsulated, which has high cost and low assembly efficiency.
  • the present disclosure provides a pipetting valve, comprising a first valve body, a second valve body and a sealing member; the second valve body is movably connected to the first valve body, and the sealing member is connected to the second valve body
  • the valve body is detachably connected, and the second valve body seals against the first valve body through the sealing member; the second valve body forms a liquid intake port, the sealing member forms a communication channel, and the The first valve body forms a liquid taking flow channel, and as the second valve body moves in the first valve body, the sealing member moves synchronously with the second valve body, and enables the liquid taking port to be able to It communicates with the liquid taking flow channel via the communication flow channel.
  • the number of the liquid taking flow channels is multiple, and the second valve body and the sealing member are configured to be able to move synchronously, so that the communication flow channels are respectively connected with a plurality of the liquid taking flow channels Pass.
  • a plurality of the liquid taking flow channels are distributed at intervals along the circumferential direction, and the second valve body is rotatably connected to the first valve body.
  • annular groove is formed on the inner wall of the first valve body; a limiting boss is formed on the outer wall of the second valve body, and the limiting boss is inserted into the annular groove.
  • the pipetting valve further includes a piston rod formed with a piston head; the piston head of the piston rod is located in the second valve body, and the piston head is sealed against the second valve body .
  • a sealing ring groove is formed on the side wall of the piston head, and a sealing ring is arranged in the sealing ring groove.
  • the sealing member is clamped to the second valve body.
  • one end of the second valve body located in the first valve body is formed with a bottom wall
  • the first valve body is formed with a bottom wall
  • the liquid extraction channel is formed on the bottom wall of the first valve body .
  • the sealing member is in the shape of a gear; the liquid intake port is located on the bottom wall of the second valve body, and the bottom wall of the second valve body forms an insertion groove, and the insertion groove is connected to the bottom wall of the second valve body.
  • the sealing member is adapted so that the sealing member is inserted into the insertion groove.
  • a flat shaft is formed on the side of the sealing member facing the second valve body; a flat groove is formed on the bottom wall of the second valve body, and the flat shaft is inserted into the flat shaft. in the slot.
  • the sealing member is formed of a sealing rubber material; an adhesive layer is provided between the sealing member and the second valve body.
  • the communication channel includes a communication hole and a diversion groove; the communication hole penetrates the sealing member, and the communication hole is communicated with the liquid intake port; the diversion groove is located in the sealing member The side of the member facing away from the second valve body, the guide groove extends along the radial direction of the sealing member, and one end of the guide groove is communicated with the liquid taking port through the communication hole, the The other end of the guide groove is configured to be able to communicate with the liquid taking channel.
  • the present disclosure also provides a sample processing device, comprising the pipetting valve described in any one of the above.
  • the present disclosure also provides a molecular diagnostic system, including the sample processing device described in any one of the above.
  • the present disclosure also provides a sample detection system, including the sample processing device described in any one of the above.
  • FIG. 1 is a schematic diagram of a split structure of a pipetting valve provided by an embodiment of the present disclosure
  • FIG. 2 is a schematic cross-sectional structure diagram of a pipetting valve provided by an embodiment of the present disclosure
  • FIG. 3 is a schematic structural diagram of a second valve body of a pipetting valve provided by an embodiment of the present disclosure
  • FIG. 4 is a schematic structural diagram of a sealant of a pipette valve provided in an embodiment of the present disclosure from a first perspective;
  • FIG. 5 is a schematic structural diagram of the sealant of the pipette valve provided by the embodiment of the present disclosure from a second perspective.
  • the terms “installed”, “connected” and “connected” should be understood in a broad sense, unless otherwise expressly specified and limited, for example, it may be a fixed connection or a detachable connection Connection, or integral connection; can be mechanical connection, can also be electrical connection; can be directly connected, can also be indirectly connected through an intermediate medium, can be internal communication between two elements.
  • installed should be understood in a broad sense, unless otherwise expressly specified and limited, for example, it may be a fixed connection or a detachable connection Connection, or integral connection; can be mechanical connection, can also be electrical connection; can be directly connected, can also be indirectly connected through an intermediate medium, can be internal communication between two elements.
  • reagent chamber should be understood in a broad sense, generally a device for storing reagents or samples (such as blood, cells, nucleic acids), etc.
  • reagents or samples such as blood, cells, nucleic acids
  • FIGS. 1 to 5 A pipetting valve and a sample processing device and a molecular diagnostic system according to some embodiments of the present disclosure are described below with reference to FIGS. 1 to 5 .
  • An embodiment of the present disclosure provides a pipetting valve, a sample processing device, and a molecular diagnostic system to achieve sealing between two valve bodies of the pipetting valve.
  • the pipetting valve includes a first valve body 1 , a second valve body 2 , a piston rod 4 and a sealing member.
  • the sealing member is Sealant 3.
  • the second valve body 2 is formed with a liquid intake port 21 that communicates with the valve cavity of the second valve body 2 , one end of the piston rod 4 is located in the valve cavity of the second valve body 2 , and the piston rod 4 is located in the second valve body 2 A piston head is formed at one end of the valve body, and the piston head seals against the valve cavity of the second valve body 2; By pushing the piston rod 4 in the second valve body 2, the reagent in the second valve body 2 can be discharged through the liquid taking port 21 of the second valve body 2; The reagent is extracted, and the reagent enters the second valve body 2 through the liquid extraction port 21 .
  • the sealant 3 is formed of a sealing rubber material.
  • the first valve body 1 forms a liquid sampling channel 11 , which is used to communicate with the reagent chamber containing reagents
  • the second valve body 2 is formed with a liquid sampling port 21 .
  • One end is movably connected to the first valve body 1, and the second valve body 2 is configured to be able to move in the first valve body 1, so that the liquid intake port 21 of the second valve body 2 and the liquid intake flow of the second valve body 2
  • the second valve body 2 is communicated with the reagent chamber; thus, when the operator pulls the piston rod 4 to the outside of the second valve body 2, the reagent in the reagent chamber can be extracted to the second valve body 2, when the operator pushes the piston rod 4 into the second valve body 2, the reagent in the second valve body 2 can be transported into the reagent chamber.
  • the sealing member includes, but is not limited to, one of sealant, sealing ring, O-ring, and gasket.
  • the material of the sealing member includes, but is not limited to, silicone and rubber, and the sealing effect is achieved by contacting with the plane through extrusion deformation.
  • the end of the second valve body 2 located in the first valve body 1 is formed with a bottom wall (ie, the bottom wall of the second valve body 2 ), and the liquid taking port 21 is formed in the first valve body 1 .
  • the bottom wall of the second valve body 2 the first valve body 1 is formed with a bottom wall (that is, the bottom wall of the first valve body 1), and the liquid intake channel 11 is formed on the bottom wall of the first valve body 1; the second valve body 2 A sealant 3 is clamped on the bottom wall of the valve body.
  • the sealant 3 is configured to move synchronously with the second valve body 2 .
  • the sealant 3 is formed with a communication channel 31 , and one end of the communication channel 31 is communicated with the liquid intake port 21 of the second valve body 2 .
  • the sealant 3 is configured to be clamped between the bottom wall of the first valve body 1 and the bottom wall of the second valve body 2, so that the first valve body 2 is The valve body 1 and the second valve body 2 are sealed against each other through the sealant 3 ; when the second valve body 2 moves to a predetermined position, the end of the communication channel 31 away from the liquid intake port 21 is configured to be able to connect with the first valve body 1 .
  • the liquid taking flow channel 11 is connected, so that the second valve body 2 is communicated with the corresponding reagent chamber containing the reagent through the communication flow channel 31 on the sealant 3 and the liquid taking flow channel 11 of the first valve body 1; thus;
  • the first valve body 1 and the second valve body 2 are sealed by the sealant 3, and the second valve body 2 is communicated with the communication channel 31 on the first valve body 1, so as to extract the reagent in the reagent chamber to the In the second valve body 2, or transfer the reagent in the second valve body 2 to the corresponding connected reagent compartment; at the same time, since the sealant 3 and the second valve body 2 are detachably connected, compared with other sealants Methods such as end face sealing between the first valve body 1 and the second valve body 2, or encapsulating the second valve body 2, and detachably connecting the sealant 3 to the second valve body 2, the implementation of the present disclosure This method not only saves the production cost, but also enables the size of the sealant 3 to be changed. While ensuring the tight
  • the first valve body 1 has a plurality of liquid-taking flow channels 11 , the plurality of liquid-taking flow channels 11 are distributed at intervals, and the plurality of liquid-taking flow channels 11 are divided into The channels 11 are respectively used to communicate with different reagent compartments.
  • the second valve body 2 is configured so that it can move to different positions in the first valve body 1, so that the liquid intake port 21 of the second valve body 2 is connected to a plurality of liquid intake streams through the communication channel 31 of the sealant 3 respectively.
  • the channel 11 communicates with each other, so that the reagent chamber corresponding to the liquid taking channel 11 can be communicated with the liquid taking port 21 of the second valve body 2 .
  • the plurality of liquid taking channels 11 of the first valve body 1 are distributed at intervals around the circumference, and the second valve body 2 is rotatably connected to the first valve body 1; when When the second valve body 2 rotates, the sealant 3 can rotate synchronously with the second valve body 2 , and when the second valve body 2 rotates to different positions, the liquid taking port 21 can be connected to a plurality of liquid taking ports respectively through the communication channel 31 .
  • the flow channel 11 is communicated with the reagent compartment corresponding to the liquid-taking flow channel 11 , so that the second valve body 2 is communicated with a plurality of reagent compartments respectively, and then reagent transfer can be performed between the plurality of reagent compartments through the second valve body 2 .
  • the communication channel 31 on the sealant 3 includes a communication hole 33 and a guide groove 34 ; the communication hole 33 penetrates the sealant 3 , and the communication hole 33 Opposite to the liquid intake port 21 on the bottom wall of the second valve body 2, the communication hole 33 is communicated with the liquid intake port 21; one end of the diversion groove 34 is communicated with the communication hole 33, and the other end of the diversion groove 34 is The radial direction of the bottom wall of the second valve body 2 extends.
  • first valve body 1 and the second valve body 2 are coaxially arranged, the liquid intake port 21 of the second valve body 2 is located at the axis of the bottom wall of the second valve body 2 , and the first valve body 1
  • the plurality of liquid taking channels 11 are distributed at circumferential intervals around the axis of the first valve body 1; the distance from the end of the guide groove 34 away from the axis of the second valve body 2 to the second axis and the end of the communication channel 31 facing the first axis
  • the distances to the first axis are the same, so that when the second valve body 2 and the sealant 3 rotate to different predetermined angles, the other ends of the guide grooves 34 can be communicated with the plurality of liquid taking channels 11 respectively.
  • the first One of the multiple liquid sampling channels 11 of the valve body 1 communicates with the first reagent chamber, and the other liquid intake channel 11 communicates with the second reagent chamber; rotating the second valve body 2 can
  • the liquid taking port 21 of the second valve body 2 is communicated with the liquid taking flow channel 11 and the first or second reagent compartment corresponding to the liquid taking flow channel 11 through the communication channel 31 of the sealant 3 respectively;
  • the second valve body 2, the sealant 3 rotates synchronously with the second valve body 2, so that the second valve body 2 is rotated to the position where it communicates with the first reagent compartment, and the piston rod 4 is pulled, and the reagent in the first reagent compartment will is extracted into the second valve body 2; then the second valve body 2 is rotated again, so that the second valve body
  • the sealant 3 is in the shape of a gear, and an insertion groove 23 is formed on the bottom wall of the second valve body 2 .
  • the groove 23 is matched with the sealant 3, so that the sealant 3 can be stably and firmly inserted into the insertion groove 23, and when the second valve body 2 rotates, the sealant 3 can follow the second valve body 2.
  • the installation between the sealant 3 and the second valve body 2 is provided with a foolproof positioning structure; optionally, as shown in FIG. 4 , the end of the sealant 3 facing the second valve body 2 is formed with a flat Position shaft 32, the bottom wall of the insertion groove 23 is formed with a flat position groove 24, and the flat position groove 24 is matched with the flat position shaft 32, so that the flat position shaft 32 can be inserted into the flat position groove 24, so as to
  • the installation between the sealant 3 and the second valve body 2 is for positioning.
  • an adhesive layer is provided between the sealant 3 and the second valve body 2, so that the sealant 3 is more stably and firmly installed on the second valve body 2, so that the sealant 3 can be It rotates synchronously with the second valve body 2 , and plays the role of sealing and connecting the second valve body 2 and the liquid taking channel 11 .
  • a piston head is formed at one end of the piston rod 4 inside the second valve body 2 , and a sealing ring groove 42 is formed on the side wall of the piston head.
  • a sealing ring 41 is installed in the sealing ring groove 42, so that the piston head is sealed against the inner wall of the second valve body 2; thus when the piston rod 4 is pulled outward, a suction force can be formed to extract the reagent to the second valve In the body 2, when the piston rod 4 is pushed into the second valve body 2, the reagent in the second valve body 2 can be sent out.
  • an annular groove 12 is formed on the inner wall of the first valve body 1
  • a limiting boss 22 is formed on the outer wall of the second valve body 2 .
  • the second valve body 2 can only rotate around its own axis in the first valve body 1; and when the second valve body 2 is clamped in the first valve body 1, the second valve body 2 and the first valve body 1 The distance between the bottom walls of the valve body 1 can compress the sealant 3 between the two, so as to achieve a better sealing effect.
  • the present disclosure also provides a sample processing device comprising the pipetting valve of any of the above embodiments.
  • the sample processing device includes a pipetting valve, and the sample processing device is used to perform nucleic acid extraction on the sample.
  • various reagents used for sample processing are transferred through the pipetting valve, so that the The sample in the sample processing device reacts with the reagent to extract nucleic acid from the sample; therefore, the sample processing device has all the beneficial effects of the pipetting valve, which will not be repeated here.
  • the sample processing device can be used to extract chemical components from the sample, such as the extraction of target proteins, target polysaccharides, target lipid compounds, target acid compounds, etc., also during the sample processing process, by pipetting
  • the valve transfers various reagents used for sample processing, so that the sample placed in the sample processing device reacts with the reagents to extract chemical components from the sample.
  • the present disclosure also provides a molecular diagnostic system, including the sample processing device of the above embodiment.
  • the molecular diagnosis system includes a sample processing device, and the molecular diagnosis system is used to complete nucleic acid detection at the molecular level on samples such as cells, hair, anticoagulation or dried blood of the subject;
  • the device completes nucleic acid extraction, and then completes the nucleic acid detection of the sample through subsequent detection steps such as nucleic acid molecular hybridization, polymerase ligation reaction (PCR), and biochip in the molecular diagnosis system, so as to prevent symptoms and signs once the disease occurs or not yet appear. and biochemical changes can be accurately detected.
  • the molecular diagnostic system includes a sample processing device, so the molecular diagnostic system has all the beneficial effects of the sample processing device, which will not be described in detail again.
  • the present disclosure also provides a sample detection system, including the sample processing device of any of the above embodiments.
  • sample detection systems include, but are not limited to, nucleic acid detection systems, chemiluminescence diagnostic systems, and biochemical analysis systems.
  • the pipetting valve provided by the present disclosure includes a first valve body, a second valve body and a sealing member; the second valve body is formed with a liquid taking port communicated with the valve cavity of the second valve body, A valve body forms a liquid extraction channel, which is used to communicate with the reagent chamber containing reagents; the second valve body formed with a liquid extraction port is movably connected to the first valve body, and the second valve body is configured In order to move in the first valve body, the liquid intake port of the second valve body is communicated with the liquid intake channel of the second valve body, and then the second valve body is communicated with the reagent chamber; The second valve body moves synchronously, the sealing member is formed with a communication channel, and one end of the communication channel is communicated with the liquid taking port of the second valve body.
  • the sealing member can be clamped between the first valve body and the second valve body, so that the first valve body and the second valve body are sealed and abutted through the sealing member;
  • the end of the communication channel away from the liquid intake port can be communicated with the liquid intake channel of the first valve body, so that the second valve body passes through the communication channel on the sealing member and the first valve body.
  • the liquid taking flow channel of the valve body is communicated with the corresponding reagent chamber containing the reagent; thus the first valve body and the second valve body are sealed by the sealing member, and the second valve body and the first valve body are sealed.
  • the communication flow channel is connected to extract the reagent in the reagent chamber into the second valve body, or transport the reagent in the second valve body to the connected reagent chamber; at the same time, because the sealing member and the second valve body are detachable
  • the sealing member is detachably connected to the second valve body , not only saves the production cost, but also enables the sealing member to be replaced in size, while ensuring the tightness between the first valve body and the second valve body, the second valve body is configured so that it can be more easily driven to operate the first valve body. body movement.
  • the sample processing device provided in the embodiments of the present disclosure includes the pipetting valve, so the sample processing device also has the beneficial effect of the pipetting valve.
  • the present disclosure also provides a molecular diagnostic system including a sample processing device, so the molecular diagnostic system also has the beneficial effects of a sample processing device.
  • the present disclosure provides a pipetting valve, which realizes the sealing between the two valve bodies of the pipetting valve.
  • the sealing member is detachably connected to the second valve body, which not only saves production
  • the cost also makes the sealing member replaceable in size, while ensuring the tightness between the first valve body and the second valve body, and configuring the second valve body to be more easily driven to move in the first valve body, has a wide range of industrial application value.
  • the present disclosure provides a sample processing device, a molecular diagnosis system and a sample detection system based on a pipetting valve, and the sample processing device also has the beneficial effects of a pipetting valve, and the molecular diagnosis system and the sample detection system also have the beneficial effects of a sample processing device. It also has great industrial application value.

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Abstract

L'invention concerne une pipette, un appareil de traitement d'échantillon, et un système de diagnostic moléculaire. La pipette comprend un premier corps de pipette (1), un second corps de pipette (2), et un élément d'étanchéité relié de manière amovible au second corps de pipette (2) ; le second corps de pipette (2) est relié de manière mobile à l'intérieur du premier corps de pipette (1) et vient en butée de manière étanche contre le premier corps de pipette (1) au moyen de l'élément d'étanchéité ; des canaux de circulation de prélèvement de liquide (11) utilisés pour communiquer avec un compartiment de réactif sont formés sur le premier corps de pipette (1) ; un orifice de prélèvement de liquide (21) est formé sur le second corps de pipette (2) ; un canal d'écoulement de communication (31) est formé sur l'élément d'étanchéité ; une extrémité du canal d'écoulement de communication (31) est en communication avec l'orifice de prélèvement de liquide (21) ; lorsque le second corps de pipette (2) se déplace vers une position prédéterminée, l'autre extrémité du canal d'écoulement de communication (31) peut être en communication avec les canaux d'écoulement de prélèvement de liquide (11), de sorte que le second corps de pipette (2) soit en communication avec le compartiment de réactif.
PCT/CN2021/106145 2020-07-14 2021-07-14 Pipette, appareil de traitement d'échantillon, et système de diagnostic moléculaire Ceased WO2022012562A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202010672666.7A CN113926499B (zh) 2020-07-14 2020-07-14 移液阀及样本处理装置和分子诊断系统
CN202010672666.7 2020-07-14

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WO2022012562A1 true WO2022012562A1 (fr) 2022-01-20

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CN116286269A (zh) * 2023-03-28 2023-06-23 重庆国科医创科技发展有限公司 一种核酸提取装置及方法

Citations (6)

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