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WO2022098681A3 - Novel class 2 crispr-cas rna-guided endonucleases - Google Patents

Novel class 2 crispr-cas rna-guided endonucleases Download PDF

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Publication number
WO2022098681A3
WO2022098681A3 PCT/US2021/057798 US2021057798W WO2022098681A3 WO 2022098681 A3 WO2022098681 A3 WO 2022098681A3 US 2021057798 W US2021057798 W US 2021057798W WO 2022098681 A3 WO2022098681 A3 WO 2022098681A3
Authority
WO
WIPO (PCT)
Prior art keywords
crispr
novel class
guided endonucleases
cas rna
type
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2021/057798
Other languages
French (fr)
Other versions
WO2022098681A2 (en
Inventor
Carla Alejandra GIMENEZ
Maria Julia Lara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Caspr Biotech Corp
Original Assignee
Caspr Biotech Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Caspr Biotech Corp filed Critical Caspr Biotech Corp
Priority to EP21815788.1A priority Critical patent/EP4240853A2/en
Priority to US17/616,121 priority patent/US20230072431A1/en
Priority to US17/541,398 priority patent/US20220136050A1/en
Priority to US17/541,405 priority patent/US20220135958A1/en
Publication of WO2022098681A2 publication Critical patent/WO2022098681A2/en
Publication of WO2022098681A3 publication Critical patent/WO2022098681A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Provided herein are novel Class 2 Type II, Type V, type VI CRISPR-Cas RNA-guided endonucleases and systems comprising the same. Provided also are methods of making, and methods of use thereof. Exemplary methods of use include modifying target nucleic acids useful for therapeutic applications, and also include detecting targeting nucleic acids, useful for diagnostic applications.
PCT/US2021/057798 2020-11-03 2021-11-03 Novel class 2 crispr-cas rna-guided endonucleases Ceased WO2022098681A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP21815788.1A EP4240853A2 (en) 2020-11-03 2021-11-03 Novel class 2 crispr-cas rna-guided endonucleases
US17/616,121 US20230072431A1 (en) 2020-11-03 2021-11-03 Novel class 2 crispr-cas rna-guided endonucleases
US17/541,398 US20220136050A1 (en) 2020-11-03 2021-12-03 Novel class 2 crispr-cas rna-guided endonucleases
US17/541,405 US20220135958A1 (en) 2020-11-03 2021-12-03 Class 2 crispr-cas rna-guided endonucleases

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063109302P 2020-11-03 2020-11-03
US63/109,302 2020-11-03

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US17/541,405 Continuation US20220135958A1 (en) 2020-11-03 2021-12-03 Class 2 crispr-cas rna-guided endonucleases
US17/541,398 Continuation US20220136050A1 (en) 2020-11-03 2021-12-03 Novel class 2 crispr-cas rna-guided endonucleases

Publications (2)

Publication Number Publication Date
WO2022098681A2 WO2022098681A2 (en) 2022-05-12
WO2022098681A3 true WO2022098681A3 (en) 2022-06-16

Family

ID=78806686

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2021/057798 Ceased WO2022098681A2 (en) 2020-11-03 2021-11-03 Novel class 2 crispr-cas rna-guided endonucleases

Country Status (1)

Country Link
WO (1) WO2022098681A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025036482A1 (en) * 2023-08-16 2025-02-20 Geneditbio Limited Type ii cas protein, crispr-cas system and uses thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018172556A1 (en) * 2017-03-24 2018-09-27 Curevac Ag Nucleic acids encoding crispr-associated proteins and uses thereof
WO2019036185A1 (en) * 2017-08-15 2019-02-21 The Regents Of The University Of California Compositions and methods for enhancing genome editing
WO2019165168A1 (en) * 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
WO2019206233A1 (en) * 2018-04-25 2019-10-31 中国农业大学 Rna-edited crispr/cas effector protein and system
WO2020168291A1 (en) * 2019-02-14 2020-08-20 Metagenomi Ip Technologies, Llc Enzymes with ruvc domains

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10253365B1 (en) 2017-11-22 2019-04-09 The Regents Of The University Of California Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018172556A1 (en) * 2017-03-24 2018-09-27 Curevac Ag Nucleic acids encoding crispr-associated proteins and uses thereof
WO2019036185A1 (en) * 2017-08-15 2019-02-21 The Regents Of The University Of California Compositions and methods for enhancing genome editing
WO2019165168A1 (en) * 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
WO2019206233A1 (en) * 2018-04-25 2019-10-31 中国农业大学 Rna-edited crispr/cas effector protein and system
WO2020168291A1 (en) * 2019-02-14 2020-08-20 Metagenomi Ip Technologies, Llc Enzymes with ruvc domains

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KOONIN EUGENE V ET AL: "Diversity, classification and evolution of CRISPR-Cas systems", CURRENT OPINION IN MICROBIOLOGY, vol. 37, 9 June 2017 (2017-06-09), pages 67 - 78, XP085276922, ISSN: 1369-5274, DOI: 10.1016/J.MIB.2017.05.008 *

Also Published As

Publication number Publication date
WO2022098681A2 (en) 2022-05-12

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