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WO2022068509A1 - Appareil de traitement de la graisse - Google Patents

Appareil de traitement de la graisse Download PDF

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Publication number
WO2022068509A1
WO2022068509A1 PCT/CN2021/116024 CN2021116024W WO2022068509A1 WO 2022068509 A1 WO2022068509 A1 WO 2022068509A1 CN 2021116024 W CN2021116024 W CN 2021116024W WO 2022068509 A1 WO2022068509 A1 WO 2022068509A1
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WO
WIPO (PCT)
Prior art keywords
fat
cleaning
liquid
another preferred
layer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2021/116024
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English (en)
Chinese (zh)
Inventor
王斌
张文杰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Seme Cell Technology Co Ltd
Original Assignee
Shanghai Seme Cell Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Seme Cell Technology Co Ltd filed Critical Shanghai Seme Cell Technology Co Ltd
Publication of WO2022068509A1 publication Critical patent/WO2022068509A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
    • B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
    • B02C18/08—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers
    • B02C18/12—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers with drive arranged below container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/04—Cleaning involving contact with liquid
    • B08B3/10—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology

Definitions

  • the invention relates to the field of biomedicine, in particular to a fat processing device.
  • the fat obtained by suction or surgical excision needs to be cleaned and shredded first.
  • the cleaning and shredding processes are often carried out separately, the operation is cumbersome, and the shredded fat Uneven particle size not only looks ugly, but also fat particles of uneven size easily lead to aggregation of fat particles and poor stability.
  • the uneven size of fat particles due to the uneven size of fat particles, the uniformity of the fat extract obtained by subsequent extraction is deteriorated. Thus, the druggability of the fat extract is limited.
  • the purpose of the present invention is to provide a fat processing device, which can effectively clean the fat and then pulverize it to obtain fat particles with uniform particle size.
  • a first aspect of the present invention provides a fat processing device, the device includes a cleaning module and a pulverizing module;
  • the cleaning module includes a cleaning device and a liquid reservoir, and the cleaning device can make the cleaning liquid clean the fat in the liquid reservoir, and after cleaning, it is left to stand for stratification, and the stratification includes a fat layer and a cleaning solution. layer;
  • the liquid reservoir is connected with the fat input pipe, the cleaning liquid input pipe, the fat output pipe and the waste liquid pipe, the fat can enter the liquid storage tank through the fat input pipe, and the cleaning liquid can be Enter into the liquid reservoir through the cleaning liquid input pipe, and the cleaning liquid layer can be discharged through the waste liquid pipe;
  • the liquid reservoir transfers the fat layer to the pulverizing module through the fat output pipe for pulverization.
  • the pulverizing module is connected to a fat outlet pipe.
  • the liquid reservoir is connected to the pulverizing module through the fat output pipe, and the fat layer can enter the pulverizing module through the fat output pipe for pulverization.
  • the liquid reservoir includes an infusion bag.
  • the cleaning device includes a rocking device.
  • the rocking device includes a rocker shaker.
  • the cleaning device includes a rocker shaker.
  • the rocking device can rock the liquid reservoir.
  • the rocking device shakes the fat in the liquid storage tank to wash the fat in the liquid storage tank.
  • the rocking device includes a tray and a push rod, and the push rod can push the tray.
  • the rocking device can rock the tray.
  • the rocking manner is rocking up and down.
  • the angle between the tray and the horizontal plane is 5-10°.
  • the frequency of the rocking is 10-60 times/min, preferably 30-50 times/min.
  • the shaking time is 1-10 min, preferably 3-8 min.
  • the push rod can push the tray to form an angle of 0-90° with the horizontal plane.
  • the push rod can push the tray to a vertical position.
  • the liquid reservoir can be located on the tray.
  • the layers include an upper fat layer and a lower cleaning liquid layer.
  • the pulverizing module is characterized in that the pulverizing module includes a pulverizing machine.
  • the pulverizer includes a pulverizing knife and a pulverizing cavity.
  • the pulverizing knife pulverizes the fat layer.
  • the shredding knife is 2-6 dry sharpening heads.
  • the head end of the crushing knife is close to the inner wall of the crushing cavity.
  • the chamber of the crushing chamber is a columnar chamber.
  • the fat output pipe can be connected to the chamber of the crushing chamber.
  • the waste liquid pipe is connected to a waste liquid collector (eg, a waste liquid collection barrel or a waste liquid collection bottle).
  • a waste liquid collector eg, a waste liquid collection barrel or a waste liquid collection bottle.
  • the fat outlet pipe is connected to a fat collector (eg, a fat collection barrel or a fat collection bottle).
  • a fat collector eg, a fat collection barrel or a fat collection bottle.
  • the fat is obtained by liposuction or surgical resection.
  • a liquid delivery device is provided on the fat input tube.
  • a liquid conveying device is provided on the cleaning liquid input pipe.
  • a liquid delivery device is provided on the fat output tube.
  • a liquid conveying device is provided on the waste liquid pipe.
  • a liquid delivery device is provided on the fat outlet pipe.
  • the liquid delivery device is a peristaltic pump.
  • the cleaning solution includes physiological saline.
  • the fat processing device comprises a rocker shaker (1), an infusion bag (2) and a pulverizer (3);
  • the rocker shaker comprises a tray (4) and a push rod (5), and the push rod can push the tray;
  • the infusion bag is located on the tray, and the infusion bag is respectively connected with the fat input pipe (6), the cleaning liquid input pipe (7), the fat output pipe (8) and the waste liquid pipe (9);
  • the pulverizer comprises a pulverizing knife (10) and a pulverizing cavity (11). close to the inner wall of the crushing chamber;
  • the pulverizing cavity is detachably connected to a fat outlet pipe (12);
  • Peristaltic pumps (13) are respectively provided on the fat input pipe, cleaning liquid input pipe, fat output pipe, waste liquid pipe and fat liquid outlet pipe.
  • the dry sharpening head is a 4-blade dry sharpening head.
  • the pulverizing cavity is detachably connected to the fat delivery tube.
  • a second aspect of the present invention provides a method for treating fat with the fat treating device according to the first aspect of the present invention, wherein the method comprises the steps of:
  • the fat enters the liquid reservoir through the fat input pipe, the cleaning liquid enters the liquid storage tank through the cleaning liquid input pipe, and the cleaning liquid cleans the fat in the liquid storage tank, and after cleaning Layering, the layering includes a fat layer and a cleaning liquid layer;
  • the fat layer enters into the pulverizing module through the fat output pipe for pulverization, so as to obtain the processed fat.
  • the fat is obtained by liposuction or surgical resection.
  • the cleaning solution is a fat cleaning solution.
  • the cleaning solution is physiological saline.
  • the volume ratio of the fat to the cleaning solution is (0.1-10): (0.1-10), preferably (0.2-5): ( 0.2-5), more preferably (0.5-3): (0.5-3), more preferably (0.5-1.5): (0.5-1.5), more preferably (0.8-1.2): (0.8-1.2), Optimally 1:1.
  • the angle between the liquid reservoir and the horizontal plane is 5-10°.
  • the liquid reservoir is located on the tray, and the angle between the tray and the horizontal plane is 5-10° during the cleaning liquid cleaning the fat in the liquid reservoir .
  • the cleaning is performed by rocking the liquid reservoir by a rocking device, and the rocking frequency is 10-60 times/min, preferably 30-50 times/min.
  • the shaking time is 1-10 min, preferably 3-8 min.
  • the cleaning is repeated cleaning.
  • the repeated cleaning times are 3-7 times.
  • the repeated cleaning includes the steps:
  • the cleaning liquid cleans the fat in the liquid reservoir, it is left to stand for stratification.
  • the stratification includes a fat layer and a cleaning liquid layer.
  • the cleaning liquid layer is discharged through a waste liquid pipe, and the cleaning liquid is passed through the cleaning liquid again.
  • the input pipe enters the liquid reservoir and is mixed with the fat layer. After the cleaning liquid cleans the fat in the liquid reservoir, it is repeated in sequence to realize the repeated cleaning of the fat.
  • step (2) after standing for stratification, the cleaning liquid layer is discharged through a waste liquid pipe.
  • step (3) the pulverization is performed by a pulverizing knife, and the rotating speed of the pulverizing knife is 5000-30000 rpm.
  • the rotating speed of the crushing knife is 5000-15000 rpm/min, preferably 8000-15000 rpm/min, more preferably 10000-13000 rpm/min, and most preferably 10000-12000 rpm/min .
  • the rotational speed of the crushing knife is 1600-30000 rpm/min, preferably 16000-25000 rpm/min, more preferably 16000-22000 rpm/min, and most preferably 17000-19000 rpm/min .
  • the grinding time is 10-40s, preferably 10-30s, preferably 15-30s, preferably 15-25s.
  • step (3) the pulverizing time is 20-25s.
  • the angle between the liquid reservoir and the horizontal plane is 70-90°, preferably 90°.
  • the angle between the tray and the horizontal plane is 70-90°, preferably 90°.
  • a fat prepared by the method according to the second aspect of the present invention.
  • FIG. 1 is a schematic structural diagram of a fat processing device in a preferred example of the present invention.
  • FIG. 2 is a schematic structural diagram of a pulverizer in a preferred example of the present invention.
  • FIG. 3 shows fats with different washing times.
  • Figure 4 shows the fat particles obtained after crushing by crushing knives at different speeds and times.
  • the inventor unexpectedly developed a fat processing device for the first time, which can effectively clean the extracted fat and remove impurities (such as tumescent fluid, blood, anesthetics, etc.) contained in the fat. etc.) to obtain fat with excellent golden yellow color, and pulverize the fat obtained after washing to obtain fat particles with excellent particle size distribution.
  • impurities such as tumescent fluid, blood, anesthetics, etc.
  • the terms "containing” or “including (including)” can be open, semi-closed and closed. In other words, the term also includes “consisting essentially of,” or “consisting of.”
  • connection or “connected” are used interchangeably and should be construed broadly, and may be fixedly connected or connected, detachably connected or connected, or integrally connected or connected.
  • the invention provides a fat processing device, the device includes a cleaning module and a pulverizing module;
  • the cleaning module includes a cleaning device and a liquid reservoir, and the cleaning device can make the cleaning liquid clean the fat in the liquid reservoir, and after cleaning, it is left to stand for stratification, and the stratification includes a fat layer and a cleaning solution. layer;
  • the liquid reservoir is connected with the fat input pipe, the cleaning liquid input pipe, the fat output pipe and the waste liquid pipe, the fat can enter the liquid storage tank through the fat input pipe, and the cleaning liquid can be Enter into the liquid reservoir through the cleaning liquid input pipe, and the cleaning liquid layer can be discharged through the waste liquid pipe;
  • the liquid reservoir transfers the fat layer to the pulverizing module through the fat output pipe for pulverization.
  • the crushing module is connected to a fat outlet pipe. After the fat washed by the crushing module is crushed, the fat enters the fat collector for collection through the fat outlet pipe.
  • the liquid reservoir is connected to the pulverizing module through the fat output pipe, and the fat layer can enter the pulverizing module through the fat output pipe for pulverization.
  • the liquid reservoir includes an infusion bag.
  • the cleaning device includes a rocking device.
  • the rocking device includes a rocker shaker.
  • the rocking device can rock the liquid reservoir.
  • the rocking device can shake the fat in the fluid reservoir so that the fat is washed in the fluid reservoir.
  • the rocking device includes a tray and a push rod, and the push rod can push the tray.
  • the reservoir may be located on the tray.
  • the rocking device can rock the tray.
  • the way of rocking is not particularly limited, and the way of rocking can be rocking up and down.
  • the angle between the tray and the horizontal plane is 5-10°.
  • the frequency of the rocking is 10-60 times/min, preferably 30-50 times/min.
  • the shaking time is 1-10 min, preferably 3-8 min.
  • the push rod can push the tray to form an angle of 0-90° with the horizontal plane.
  • the push rod can push the tray to a vertical position.
  • the layers include an upper fat layer and a lower cleaning liquid layer.
  • the pulverizing module includes a pulverizing machine.
  • the pulverizer includes a pulverizing knife and a pulverizing cavity.
  • the pulverizing knife pulverizes the fat layer.
  • the shredding knife is 2-6 dry sharpening heads.
  • the head end of the crushing knife is close to the inner wall of the crushing cavity.
  • the chamber of the crushing chamber is a columnar chamber.
  • the fat output pipe can be connected to the chamber of the crushing chamber.
  • the waste liquid pipe is connected to a waste liquid collector (eg, a waste liquid collection barrel or a waste liquid collection bottle).
  • a waste liquid collector eg, a waste liquid collection barrel or a waste liquid collection bottle.
  • the fat outlet pipe is connected to a fat collector (eg, a fat collection barrel or a fat collection bottle).
  • a fat collector eg, a fat collection barrel or a fat collection bottle.
  • the present invention provides a fat processing device, the fat processing device comprising a rocker shaker (1), an infusion bag (2) and a pulverizer (3);
  • the rocker shaker comprises a tray (4) and a push rod (5), and the push rod can push the tray;
  • the infusion bag is located on the tray, and the infusion bag is respectively connected with the fat input pipe (6), the cleaning liquid input pipe (7), the fat output pipe (8) and the waste liquid pipe (9);
  • the pulverizer comprises a pulverizing knife (10) and a pulverizing cavity (11). close to the inner wall of the crushing chamber;
  • the pulverizing cavity is detachably connected to a fat outlet pipe (12);
  • Peristaltic pumps (13) are respectively provided on the fat input pipe, cleaning liquid input pipe, fat output pipe, waste liquid pipe and fat liquid outlet pipe.
  • the dry sharpening head is a 4-blade dry sharpening head.
  • the pulverizing cavity is detachably connected to the fat delivery tube.
  • the present invention also provides a method for treating fat with the fat treating device according to the present invention, the method comprising the steps of:
  • the fat enters the liquid reservoir through the fat input pipe, the cleaning liquid enters the liquid storage tank through the cleaning liquid input pipe, and the cleaning liquid cleans the fat in the liquid storage tank, and after cleaning Layering, the layering includes a fat layer and a cleaning liquid layer;
  • the fat layer enters into the pulverizing module through the fat output pipe for pulverization, so as to obtain the processed fat.
  • the fat is obtained by liposuction or surgical resection.
  • the volume ratio of the fat to the cleaning solution is (0.1-10): (0.1-10), preferably (0.2-5): (0.2-5), more preferably (0.5-3): (0.5-3), more preferably (0.5-1.5): (0.5-1.5), more preferably (0.8-1.2): (0.8-1.2), most preferably 1:1.
  • the angle between the liquid reservoir and the horizontal plane is 5-10°.
  • the liquid reservoir is located on the tray, and during the cleaning liquid cleaning the fat in the liquid reservoir, the angle between the tray and the horizontal plane is 5-10° .
  • the repeated cleaning includes the steps:
  • the cleaning liquid cleans the fat in the liquid reservoir, it is left to stand for stratification.
  • the stratification includes a fat layer and a cleaning liquid layer.
  • the cleaning liquid layer is discharged through a waste liquid pipe, and the cleaning liquid is passed through the cleaning liquid again.
  • the input pipe enters the liquid reservoir and is mixed with the fat layer. After the cleaning liquid cleans the fat in the liquid reservoir, it is repeated in sequence to realize the repeated cleaning of the fat.
  • step (2) after standing for stratification, the cleaning liquid layer is discharged through a waste liquid pipe.
  • step (3) the pulverization is performed by a pulverizing knife.
  • the rotational speed of the pulverizing knife is 5000-30000 revolutions/min.
  • the rotating speed of the crushing knife is 5000-15000 rpm/min, preferably 8000-15000 rpm/min, more preferably 10000-13000 rpm/min, and most preferably 10000-12000 rpm/min .
  • the rotating speed of the crushing knife is 1600-30000 rpm/min, preferably 16000-25000 rpm/min, more preferably 16000-22000 rpm/min, and most preferably 17000-19000 rpm/min .
  • the grinding time is 10-40s, preferably 10-30s, preferably 15-30s, preferably 15-25s.
  • step (3) the pulverizing time is 20-25s.
  • the angle between the liquid reservoir and the horizontal plane is 70-90°, preferably 90°.
  • the angle between the tray and the horizontal plane is 70-90°, preferably 90°.
  • the fat processing device of the present invention can effectively clean the extracted fat, remove impurities (such as tumescent fluid, blood, anesthetics, etc.) contained in the fat, and obtain fat with excellent golden yellow color.
  • the fat is pulverized to obtain fat particles with excellent particle size distribution.
  • the fat processing device of the present invention is simple in structure, convenient in operation, and easy in industrialized production.
  • This embodiment 1 provides a fat processing device, the device is shown in combination with FIG. 1 and FIG. 2 , and the device includes a rocker shaker (1), an infusion bag (2) and a pulverizer (3);
  • the rocker shaker comprises a tray (4) and a push rod (5), and the push rod can push the tray;
  • the infusion bag is located on the tray, and the infusion bag is respectively connected with the fat input pipe (6), the cleaning liquid input pipe (7), the fat output pipe (8) and the waste liquid pipe (9);
  • the pulverizer comprises a pulverizing knife (10) and a pulverizing cavity (11).
  • the head end is close to the inner wall of the crushing chamber;
  • the pulverizing cavity is detachably connected to a fat outlet pipe (12);
  • Peristaltic pumps (13) are respectively provided on the fat input pipe, cleaning liquid input pipe, fat output pipe, waste liquid pipe and fat liquid outlet pipe.
  • This embodiment 2 uses the fat treatment device described in embodiment 1 to process fat, and the method includes the steps:
  • the push rod pushes the tray, so that the angle between the tray and the horizontal plane is 90°, and the layers are left to stand again, and the layers are the upper fat layer and the lower cleaning liquid layer. 4 times to achieve repeated cleaning of the fat to obtain the cleaned fat layer;
  • the washed fat layer enters into the crushing cavity of the crusher through the fat output pipe, the crushing knife crushes the fat, and after crushing, the processed fat is collected through the fat outlet pipe.
  • step (2) the fat emulsion with different cleaning times is shown in FIG. 3 .
  • step (3) the fat obtained after pulverizing knives with different rotation speeds and times is thinly spread on the bottom of the petri dish, and the microscopic observation is photographed. It is 11000 rev/min, and the second gear speed of the crushing knife is 18000 rev/min.
  • the retention rate of uniformly distributed fat particles after transplantation is more stable, and at the same time, uniformly distributed fat particles are more convenient for subsequent processing to prepare adipose tissue derivatives, which can improve and stabilize the quality and stability of adipose tissue-derived products. yield, etc.

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Disintegrating Or Milling (AREA)

Abstract

L'invention concerne un appareil de traitement de la graisse, comprenant un module de nettoyage et un module de broyage. Le module de nettoyage comprend un moyen de nettoyage et un réservoir de liquide ; le moyen de nettoyage peut nettoyer la graisse dans le réservoir de liquide à l'aide d'une solution de nettoyage, et après le nettoyage, le réservoir de liquide est placé toujours en couches ; les couches comprennent une couche de graisse et une couche de solution de nettoyage ; le réservoir de liquide est relié à un tube d'entrée de graisse (6), un tube d'entrée de solution de nettoyage (7), un tube de sortie de graisse (8), et un tube de déchet liquide (9) ; la graisse entre dans le réservoir de liquide à travers le tube d'entrée de graisse (6) ; la solution de nettoyage entre dans le réservoir de liquide à travers le tube d'entrée de solution de nettoyage (7) ; la couche de solution de nettoyage est évacuée par le tube de déchets liquides (9) ; le réservoir de liquide transfère, au moyen du tube de sortie de graisse (8), la couche de graisse au module de broyage pour le broyage. L'appareil peut nettoyer la graisse extraite et broyer la graisse nettoyée pour obtenir des particules de graisse ayant une excellente distribution de taille de particule.
PCT/CN2021/116024 2020-09-29 2021-09-01 Appareil de traitement de la graisse Ceased WO2022068509A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202011056200.0 2020-09-29
CN202011056200.0A CN114308858A (zh) 2020-09-29 2020-09-29 一种脂肪处理装置

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WO2022068509A1 true WO2022068509A1 (fr) 2022-04-07

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WO2025201569A1 (fr) * 2024-03-29 2025-10-02 上海韬睿生物科技有限公司 Procédé et appareil de détection de performance d'étanchéité de tube après installation, et produit-programme

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JP2007289076A (ja) * 2006-04-25 2007-11-08 Kaneka Corp 脂肪組織から幹細胞を採取するのに適した細胞分離装置、およびその方法
WO2014154990A2 (fr) * 2013-03-25 2014-10-02 Groupe Sebbin Sas Dispositif et procede de traitement de cellules graisseuses, notamment pour nettoyer et filtrer les cellules graisseuses d'un patient en vue d'un lipofilling
CN204509328U (zh) * 2015-03-23 2015-07-29 杭州百桥医疗技术有限公司 一种脂肪组织清洗纯化装置
CN209564684U (zh) * 2018-12-27 2019-11-01 北京佳士力科技有限公司 一种自体脂肪分离处理装置
CN110354572A (zh) * 2019-07-23 2019-10-22 徐晓婷 自体脂肪分离提纯设备及方法
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CN110813091A (zh) * 2019-11-16 2020-02-21 余春焰 一种利于脂肪移植的脂肪提纯方法

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