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WO2021207869A1 - Preparation method for starch-lipid-protein complex, and application thereof - Google Patents

Preparation method for starch-lipid-protein complex, and application thereof Download PDF

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Publication number
WO2021207869A1
WO2021207869A1 PCT/CN2020/084398 CN2020084398W WO2021207869A1 WO 2021207869 A1 WO2021207869 A1 WO 2021207869A1 CN 2020084398 W CN2020084398 W CN 2020084398W WO 2021207869 A1 WO2021207869 A1 WO 2021207869A1
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Prior art keywords
starch
lipid
fat
protein complex
protein
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French (fr)
Chinese (zh)
Inventor
王书军
晁琛
黄世清
于璟林
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Tianjin University of Science and Technology
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Tianjin University of Science and Technology
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Priority to CN202080099381.7A priority Critical patent/CN115427496B/en
Priority to PCT/CN2020/084398 priority patent/WO2021207869A1/en
Publication of WO2021207869A1 publication Critical patent/WO2021207869A1/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23GCOCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G9/00Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor
    • A23G9/32Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/10Foods or foodstuffs containing additives; Preparation or treatment thereof containing emulsifiers
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/206Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
    • A23L29/212Starch; Modified starch; Starch derivatives, e.g. esters or ethers
    • A23L29/219Chemically modified starch; Reaction or complexation products of starch with other chemicals
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08KUse of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K5/00Use of organic ingredients
    • C08K5/04Oxygen-containing compounds
    • C08K5/09Carboxylic acids; Metal salts thereof; Anhydrides thereof
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08LCOMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L3/00Compositions of starch, amylose or amylopectin or of their derivatives or degradation products
    • C08L3/02Starch; Degradation products thereof, e.g. dextrin
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08LCOMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L89/00Compositions of proteins; Compositions of derivatives thereof

Definitions

  • the invention belongs to the technical field of food processing, and specifically relates to a preparation method and application of a starch-lipid-protein complex.
  • Biochemical reagents lead to high safety risks; from a nutritional point of view, the enzyme hydrolyzed/acid hydrolyzed starch has very high digestibility, which easily stimulates the human body to secrete large amounts of insulin in a short time, which causes a huge burden on human health .
  • the purpose of the present invention is to provide a method for preparing a starch-lipid-protein complex.
  • Medium starch can form starch-lipid-protein complexes with lipids (fatty acids, monoglycerides, etc.) and proteins, thereby significantly changing the structure and functional properties of starch.
  • the formation of complexes can inhibit the swelling and gelatinization of starch particles, and reduce Starch gel strength, improve starch paste viscosity, plasticity and spreadability, and inhibit starch aging.
  • this complex since this complex has a significant V-shaped single helix ordered structure, it is more difficult to be hydrolyzed by enzymes and not easily digested by the small intestine.
  • the starch-lipid-protein complex After the starch-lipid-protein complex is rehydrated as a fat mimic, it can simulate the physical properties and sensory characteristics of natural cream. At the same time, the lower digestive properties of the compound itself also endow it with higher nutritional value, and it is a new type of dietary fiber that is more easily accepted by consumers. Therefore, we propose that the compound can be used as a fat mimic to completely replace or partially replace the fat in frozen or baked foods, reduce the body's intake of high-calorie fat, prevent various chronic diseases caused by excessive fat intake, and improve human health. Ice cream is a popular food, but due to its high fat content, it limits the consumption of ice cream by many people, especially those with diabetes and obesity. Grease also plays an important role in food processing and nutritional functions. It gives food a lubricating taste, unique flavor, specific tissue state and good stability.
  • the present invention is to provide a fat simulant, which has the advantages of high viscosity, weak gel strength, and good spreadability. When applied to ice cream, it can effectively reduce the fat content in the ice cream without affecting the shape and shape of the ice cream.
  • the taste and flavor have a greater impact, the preparation process is simple and feasible, the raw material cost is low, the preparation conditions are easy to control, and the resulting product is safe and non-toxic, which is beneficial to the realization of the industrial production of low-fat ice cream.
  • step (3) The composite sample obtained in step (2) is freeze-dried for 24 hours, crushed, and passed through an 80-mesh sieve.
  • the starch selected in the step (1) is non-waxy starch
  • the lipid is fatty acid, monoglyceride
  • the protein is water-soluble protein. Because the formation of the complex mainly relies on amylose in starch, and waxy starch does not contain amylose, non-waxy starch is selected.
  • the non-waxy starch is common vegetable starch such as wheat, corn, potato.
  • the preparation of low-fat ice cream includes the following steps:
  • step (3) Heating the mixed material liquid obtained in step (3) at 60-70°C for 20-30 minutes to obtain a sterilized material liquid;
  • step (7) Put the mature liquid obtained in step (6) into an ice cream freezer to freeze and form, and harden at -18-20°C for 6-24 hours to obtain low-fat ice cream.
  • the starch-lipid-protein complex of the present invention can also be used as a food ingredient in the preparation of bread, steamed bread and noodles to improve their quality.
  • This phenomenon has prolonged the shelf life; when the sample is rehydrated and stirred evenly during use, the sample has no peculiar smell, and can well simulate the shape and texture of cream products, reducing or even replacing the cream content in ice cream and other high-fat foods, significantly making up for The problem of lack of taste and flavor due to reduced fat content; by adjusting the types and proportions of lipids and water-soluble proteins, fat simulants with different textures can be obtained, reducing or even replacing cream products, which is conducive to the industrial production of low-fat foods .
  • the fat simulant of the present invention has the advantages of simple and feasible preparation process, low cost of raw materials, not easy to be digested and absorbed by the small intestine, no biochemical reagents, and safety and non-toxic characteristics of the obtained product, which overcomes the high cost of existing starch-based fat simulants ,
  • the reaction process is complicated and the lack of high digestibility. It can be used in the preparation of low-fat ice cream and can replace 50% of the fat content in ordinary cream ice cream.
  • skimmed milk powder 101.2g of skimmed milk powder, 120g of white sugar, 139.3g of cream, 250g of fat simulant, 2g of sodium alginate, 1.5g of monoglyceride, and 386g of purified water.
  • the method for preparing the aforementioned low-fat ice cream includes the following steps:
  • step (3) Heating the mixed material liquid obtained in step (3) at 70° C. for 30 minutes to obtain a sterilized material liquid;
  • step (6) Put the mature material liquid obtained in step (6) into an ice cream freezer to freeze and form, and harden at -18°C for 24 hours to obtain a low-fat ice cream.
  • step (2) Fully stir the suspension obtained in step (1) uniformly, preheat at 50°C for 1 min, heat up to 95°C and then heat for 2.5 min, then lower the temperature to 50°C and keep it for 2 min, the whole process is always accompanied by stirring;
  • step (2) Fully stir the suspension obtained in step (1) uniformly, preheat at 50°C for 1 min, heat up to 95°C and then heat for 2.5 min, then lower the temperature to 50°C and keep it for 2 min, the whole process is always accompanied by stirring;
  • step (3) The composite sample obtained in step (2) is freeze-dried for 24 hours, crushed, and passed through an 80-mesh sieve to obtain a starch-lipid-protein complex.
  • step (2) Fully stir the suspension obtained in step (1) evenly, preheat at 50°C for 1 min, heat up to 95°C for 2.5 min, then lower the temperature to 50°C and keep it for 2 min, the whole process is always accompanied by stirring;
  • step (2) Fully stir the suspension obtained in step (1) uniformly, preheat at 50°C for 1 min, heat up to 95°C and then heat for 2.5 min, then lower the temperature to 50°C and keep it for 2 min, the whole process is always accompanied by stirring;
  • step (3) The composite sample obtained in step (2) is freeze-dried for 24 hours, crushed and passed through an 80-mesh sieve to obtain a starch-lipid-protein complex.
  • This comparative example is to prepare ordinary cream ice cream without replacing fat, which is made from the following formula:
  • skimmed milk powder 120g of white sugar, 282g of cream, 2g of sodium alginate, 1.5g of monoglyceride, and 493g of purified water.
  • This comparative example is to prepare cream-free ice cream, which is made from the following formula:
  • skimmed milk powder 120g of white sugar, 2g of sodium alginate, 1.5g of monoglyceride, and 775.3g of purified water.
  • This comparative example is to prepare a low-fat ice cream containing a fat mimic of a starch-lipid complex as a matrix.
  • step (2) Fully stir the suspension obtained in step (1) uniformly, preheat at 50°C for 1 min, heat up to 95°C and then heat for 2.5 min, then lower the temperature to 50°C and keep it for 2 min, the whole process is always accompanied by stirring;
  • step (3) The composite sample obtained in step (2) is freeze-dried for 24 hours, crushed and passed through an 80-mesh sieve to obtain a starch-lipid-protein complex.
  • the X-ray diffractometer (D8-ADVANCE) of Bruker, Germany was used to detect the crystal structure of fat mimics with starch-lipid and starch-lipid-protein complex as the matrix ( Figure 3).
  • the measurement results show that the two fat mimics both exhibit unique V-shaped crystal diffraction peaks (12.9° and 19.8°), indicating that they are mainly composed of V-shaped complexes with good enzymolysis resistance.
  • the differential scanning calorimeter (200F3) of Netzsch, Germany was used to analyze the thermodynamic properties of fat simulants with starch-lipid and starch-lipid-protein complexes as the matrix (Table 1).
  • the analysis results showed that the two fat simulants
  • T p The melting temperature (T p ) is higher than 90 °C, with good thermal stability.
  • a 1.5% (w/w) fat simulant sample was dispersed in distilled water, mixed with 5% soybean oil at room temperature, and homogenized using a high-speed homogenizer at 12000 rpm for 3 minutes to form a uniform emulsion. Take 50 ⁇ L of emulsion and mix it with 5mL 0.1% SDS (sodium dodecyl sulfate), and use 5mL 0.1% SDS as a blank to measure the absorbance value of the sample at a wavelength of 500nm. The obtained value can characterize the emulsification activity of the sample.
  • SDS sodium dodecyl sulfate
  • Determination of apparent viscosity of ice cream melt the ice cream at room temperature, use MCR302 rheometer to measure, the measuring rotor model is PP50 (diameter 50mm), drop the ice cream material onto the lower test plate of the rheometer, measure the rotor and test The clearance of the lower plate is 1mm. Set the shear rate to 10rad/s for measurement.
  • Ice cream hardness measurement The ice cream sample in a 2.5oz paper cup is frozen at -20°C for 24 hours and then immediately placed in an environment of 25°C for hardness measurement.
  • the pre-test rate is 1mm/s
  • the test rate is 1mm/s
  • the post-test rate is 10mm/s
  • the test depth is 15mm
  • the trigger stress is 10g.
  • the fat mimics with starch-lipid-protein complex as matrix have good thermal stability and unique V-shaped crystal structure, and compared with starch-lipid complex as matrix fat
  • the simulant has lower gel strength, better spreadability, emulsification and water and oil retention. Adding it as a food ingredient to ice cream to replace fat can make the system more uniform and stable, which is more uniform and stable than cream-free ice cream and starch -The ice cream prepared by the fat mimic of the lipid complex as the matrix is closer to the various physical and chemical indexes and sensory properties of ordinary cream ice cream.

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  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Inorganic Chemistry (AREA)
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Abstract

A preparation method for a starch-lipid-protein complex, and an application thereof. The preparation of the complex comprises the following steps: (1) adding a water-soluble protein in a starch and lipid system, heating, by means of controlling a water temperature, a mixture system until starch is gelatinized, and then performing cooling to obtain a complex sample; and (2) grinding the complex into powder after freeze-drying, and storing same. The complex can be subjected to rehydration to obtain a fat mimic; the fat mimic has a physical property similar to cream, is simple in preparation process, high in heat stability, low in glycemic index, has a potential function of regulating intestinal health of a human body, and can be applied to ice cream for replacing part of cream, thereby reducing the fat content and reducing the damage to human health due to excessive calories thereof. Moreover, a sensory evaluation result shows that the addition of the fat mimic can effectively compensate for the loss of taste and flavor of the ice cream caused by the reduction of fat content. The complex may be further used as a food ingredient for food processing.

Description

一种淀粉-脂质-蛋白质复合物的制备方法及其应用Preparation method and application of starch-lipid-protein complex 技术领域Technical field

本发明属于食品加工技术领域,具体涉及一种淀粉-脂质-蛋白质复合物的制备方法及其应用。The invention belongs to the technical field of food processing, and specifically relates to a preparation method and application of a starch-lipid-protein complex.

背景技术Background technique

随着经济与社会的快速发展,人们在注重食物本身风味及口感的同时也更加关注饮食健康。脂肪作为我们日常膳食的主要营养成分之一,在人类饮食生活中发挥着非常重要的作用,一方面它赋予了食物诱人的风味、优良的口感以及良好的感官特性,另一方面它也是人体能量、必需脂肪酸和脂溶性维生素的重要来源。然而近年来,全球居民的脂肪摄入量呈逐年上升趋势,因脂肪摄入量过高引发的癌症、心脑血管疾病以及糖尿病等慢性疾病高发,严重阻碍了人们的工作生活以及社会的发展。因此在很好的保持食物的风味、口感等感官性质的同时尽可能多的取代食品中的高热量油脂,减少脂肪摄入,已成为食品科学领域的研究热点,而脂肪模拟物就是这样一种应时而生的拥有巨大应用潜力的脂肪替代品。With the rapid development of economy and society, people pay more attention to the health of the diet while paying attention to the flavor and taste of the food itself. As one of the main nutrients of our daily diet, fat plays a very important role in human dietary life. On the one hand, it gives food attractive flavor, excellent taste and good sensory characteristics. On the other hand, it is also a human body. An important source of energy, essential fatty acids and fat-soluble vitamins. However, in recent years, the fat intake of global residents has been increasing year by year. The high incidence of chronic diseases such as cancer, cardiovascular and cerebrovascular diseases and diabetes caused by excessive fat intake has seriously hindered people's work, life and social development. Therefore, it has become a research hotspot in the field of food science to replace the high-calorie fat in food as much as possible while maintaining the sensory properties such as the flavor and mouthfeel of the food. A fat substitute with great application potential is born from time to time.

以淀粉为基质的脂肪模拟物原料常来源于马铃薯、木薯、玉米、小麦等,目前最常见的方法是将淀粉经稀酸或淀粉酶处理至DE值(葡萄糖当量)小于5,使其能够模拟脂肪的感官特性。这主要是由于脂肪模拟物形成的三维网状凝胶结构能截留大量水分子,从而使脂肪模拟物具有很好的流动性,在质感和口感上与脂肪类似,产生奶油状的润滑感和粘稠度以及良好的涂抹性和假塑性。目前基于淀粉制备脂肪模拟物的研究大都基于酶/酸水解工艺,通过改良产物的保水性能来模拟脂肪的物理特性。从加工及经济角度分析,但这类脂肪模拟物会导致产品含水量过高,对产品贮藏产生不利影响,在贮藏和销售过程中易发生析水,并且成本昂贵、工艺复杂且利用到多种生物化学试剂,导致其具有较高的安全隐患;从营养角度分析,酶解/酸解的淀粉具有非常高的消化性,容易刺激人体胰岛素在短时间大量分泌,从而对人体健康造成巨大的负担。The raw materials of starch-based fat simulants are often derived from potatoes, cassava, corn, wheat, etc. At present, the most common method is to treat starch with dilute acid or amylase until the DE value (dextrose equivalent) is less than 5, so that it can simulate The sensory properties of fat. This is mainly because the three-dimensional network gel structure formed by the fat simulant can intercept a large amount of water molecules, so that the fat simulant has good fluidity, and is similar to fat in texture and taste, resulting in a creamy lubricity and stickiness. Consistency and good spreadability and pseudoplasticity. At present, most of the researches on preparing fat simulants based on starch are based on enzyme/acid hydrolysis process, which simulates the physical properties of fat by improving the water retention properties of the product. Analyzed from the perspective of processing and economics, this type of fat simulant will cause excessive moisture content in the product, which will have an adverse effect on product storage. It is prone to water separation during storage and sales, and is expensive, complex in technology, and can be used in a variety of ways. Biochemical reagents lead to high safety risks; from a nutritional point of view, the enzyme hydrolyzed/acid hydrolyzed starch has very high digestibility, which easily stimulates the human body to secrete large amounts of insulin in a short time, which causes a huge burden on human health .

因此现在需要一种制备方法简单,不使用生物化学试剂,且在口感、形态方面更接近脂肪的模拟物。Therefore, there is now a need for a simulant that has a simple preparation method, does not use biochemical reagents, and is closer to fat in terms of taste and shape.

发明内容Summary of the invention

因此,为了克服现有技术和营养价值的不足,本发明的目的在于提供一种淀粉-脂质-蛋白质复合物的制备方法,以其为基质的脂肪模拟物的制备方法,我们发现热加工过程中淀粉可以与脂质(脂肪酸、单甘酯等)和蛋白质形成淀粉-脂质-蛋白质复合物,从而显著改变淀粉的结构及功能性质,复合物的形成能够抑制淀粉颗粒溶胀及糊化、降低淀粉凝胶强度、提高淀粉糊粘度、可塑性及涂抹性并抑制淀粉老化。此外,由于这种复合物具有显著的V-型单螺旋有序结构,其 较难被酶水解且不易被小肠消化。正是由于复合物这种独特的功能性质及营养特性,使其可以作为一种新型的食用淀粉资源广泛的用于食品加工及生产,本发明制备的淀粉-脂质-蛋白质复合物可以作为食品成分用于面包、馒头和面条的制备以改善其品质。将其复水后可以作为脂肪模拟物。Therefore, in order to overcome the shortcomings of the prior art and nutritional value, the purpose of the present invention is to provide a method for preparing a starch-lipid-protein complex. Medium starch can form starch-lipid-protein complexes with lipids (fatty acids, monoglycerides, etc.) and proteins, thereby significantly changing the structure and functional properties of starch. The formation of complexes can inhibit the swelling and gelatinization of starch particles, and reduce Starch gel strength, improve starch paste viscosity, plasticity and spreadability, and inhibit starch aging. In addition, since this complex has a significant V-shaped single helix ordered structure, it is more difficult to be hydrolyzed by enzymes and not easily digested by the small intestine. It is precisely because of the unique functional properties and nutritional properties of the complex that it can be used as a new type of edible starch resource and widely used in food processing and production. The starch-lipid-protein complex prepared by the present invention can be used as a food The ingredients are used in the preparation of bread, steamed bread and noodles to improve their quality. After being rehydrated, it can be used as a fat mimic.

淀粉-脂质-蛋白质复合物复水后作为脂肪模拟物能够模拟天然奶油的物理性质及感官特性。同时,复合物自身的较低消化特性也赋予了其较高营养价值,是一种较容易被消费者所接受的新型膳食纤维。因此我们提出复合物可作为脂肪模拟物完全替代或部分替代冷冻或焙烤食品中的脂肪,降低人体对高热量脂肪的摄入,预防因脂肪过量摄入导致的各种慢性疾病,改善人体健康。冰淇淋是大众喜爱的食品,但由于其脂肪含量高,限制了很多人对冰淇淋的消费,尤其是糖尿病以及肥胖人群。而油脂又在食品加工和营养功能中都起着重要作用,它赋予了食品润滑口感、独特风味、特定组织状态和良好稳定性。After the starch-lipid-protein complex is rehydrated as a fat mimic, it can simulate the physical properties and sensory characteristics of natural cream. At the same time, the lower digestive properties of the compound itself also endow it with higher nutritional value, and it is a new type of dietary fiber that is more easily accepted by consumers. Therefore, we propose that the compound can be used as a fat mimic to completely replace or partially replace the fat in frozen or baked foods, reduce the body's intake of high-calorie fat, prevent various chronic diseases caused by excessive fat intake, and improve human health. Ice cream is a popular food, but due to its high fat content, it limits the consumption of ice cream by many people, especially those with diabetes and obesity. Grease also plays an important role in food processing and nutritional functions. It gives food a lubricating taste, unique flavor, specific tissue state and good stability.

本发明要提供一种脂肪模拟物,其具有粘度高、凝胶强度弱、涂抹性好等优点,将其应用于冰淇淋中,既能够有效降低冰淇淋中脂肪含量,又不会对冰淇淋的形态、口感、风味造成较大的影响,具备制备工艺简单可行,原料成本价格低廉,制备条件容易控制,所得产品安全无毒的特点,有利于实现低脂冰淇淋的工业化生产。The present invention is to provide a fat simulant, which has the advantages of high viscosity, weak gel strength, and good spreadability. When applied to ice cream, it can effectively reduce the fat content in the ice cream without affecting the shape and shape of the ice cream. The taste and flavor have a greater impact, the preparation process is simple and feasible, the raw material cost is low, the preparation conditions are easy to control, and the resulting product is safe and non-toxic, which is beneficial to the realization of the industrial production of low-fat ice cream.

本发明是通过如下技术方案来实现上述目的的。The present invention achieves the above objectives through the following technical solutions.

一种淀粉-脂质-蛋白质复合物的制备方法,包括如下步骤:A preparation method of starch-lipid-protein complex includes the following steps:

(1)将淀粉与脂质混合,然后在淀粉-脂质体系中加入蛋白质,加入足量纯净水,配置成10wt%的悬浮液;(1) Mix starch and lipids, then add protein to the starch-lipid system, add sufficient purified water to prepare a 10wt% suspension;

(2)将步骤(1)得到的悬浮液充分搅拌均匀,加热至淀粉凝胶化后冷却;(2) Stir the suspension obtained in step (1) thoroughly, heat it until the starch is gelatinized and then cool;

(3)将步骤(2)得到的复合物样品冷冻干燥24h,搅碎后过80目筛。(3) The composite sample obtained in step (2) is freeze-dried for 24 hours, crushed, and passed through an 80-mesh sieve.

优选的,所述步骤(1)中脂质添加量为淀粉干基重量的2-5%,蛋白质添加量为淀粉干基重量的2-10%。Preferably, in the step (1), the added amount of lipid is 2-5% of the dry basis weight of starch, and the added amount of protein is 2-10% of the dry basis weight of starch.

优选的,所述步骤(1)选用的淀粉为非蜡质淀粉,脂质为脂肪酸、单甘酯,蛋白质为水溶性蛋白质。因为形成复合物主要靠淀粉中的直链淀粉,蜡质淀粉中不含直链淀粉,所以选用的是非蜡质淀粉。Preferably, the starch selected in the step (1) is non-waxy starch, the lipid is fatty acid, monoglyceride, and the protein is water-soluble protein. Because the formation of the complex mainly relies on amylose in starch, and waxy starch does not contain amylose, non-waxy starch is selected.

优选的,非蜡质淀粉是普通植物性淀粉如小麦,玉米,马铃薯。Preferably, the non-waxy starch is common vegetable starch such as wheat, corn, potato.

优选的,所述单甘脂为单肉豆蔻酸甘油酯,单硬脂酸甘油酯。Preferably, the monoglyceride is glycerol monomyristate, glycerol monostearate.

优选的,所述水溶性蛋白质为β-乳球蛋白。Preferably, the water-soluble protein is β-lactoglobulin.

优选的,所述步骤(2)的加工方式为:在40-50℃预热1-3min,升温到90-100℃后加热2-10min,随后降温到20-50℃并保持2-5min,整个加工过程一直伴随着搅拌。Preferably, the processing method of the step (2) is: preheating at 40-50°C for 1-3min, heating to 90-100°C and heating for 2-10min, then cooling to 20-50°C and keeping it for 2-5min, The whole process has been accompanied by stirring.

一种淀粉-脂质-蛋白质复合物为基质的脂肪模拟物应用,用于制备低脂冰淇淋。冰激凌各组分质量百分比为:脱脂奶粉9-11%,白砂糖12-14%,奶油5-25%,脂肪模拟物10-40%,海藻酸钠0.1-0.3%,单甘酯0.1-0.3%,水余量。A starch-lipid-protein complex is used as a matrix fat mimic application for preparing low-fat ice cream. The mass percentage of each component of ice cream is: skimmed milk powder 9-11%, white sugar 12-14%, cream 5-25%, fat simulant 10-40%, sodium alginate 0.1-0.3%, monoglyceride 0.1-0.3 %, the remaining amount of water.

用于制备低脂冰淇淋包括如下步骤:The preparation of low-fat ice cream includes the following steps:

(1)向搅拌缸中加入部分水,加热至30-45℃后将脱脂奶粉放入搅拌缸中,充分搅拌使其溶解;(1) Add part of the water to the mixing tank, heat it to 30-45°C, put the skimmed milk powder into the mixing tank, and stir fully to dissolve it;

(2)将淀粉-脂质-蛋白质复合物复水得到脂肪模拟物,将奶油及脂肪模拟物预混后加入到搅拌缸中,充分搅拌均匀;复水后脂肪模拟物水分含量为90%;(2) Rehydrate the starch-lipid-protein complex to obtain a fat simulant, premix the cream and the fat simulant into the mixing tank, and stir well; the moisture content of the fat simulant after rehydration is 90%;

(3)将糖溶解在剩余的水中,向其中缓慢加入海藻酸钠和单甘酯,待充分溶解后缓慢倒入搅拌缸,充分搅拌,得到混合料液;(3) Dissolve the sugar in the remaining water, slowly add sodium alginate and monoglyceride to it, slowly pour it into the mixing tank after it is fully dissolved, and stir it fully to obtain a mixed material liquid;

(4)将步骤(3)中得到的混合料液在60-70℃条件下加热20-30min,获得灭菌料液;(4) Heating the mixed material liquid obtained in step (3) at 60-70°C for 20-30 minutes to obtain a sterilized material liquid;

(5)将步骤(4)获得的灭菌料液进行高压均质,获得均质料液;(5) Perform high-pressure homogenization of the sterilized material liquid obtained in step (4) to obtain a homogeneous material liquid;

(6)将步骤(5)得到的均质料液冷却到2-4℃后进行老化,老化温度为2-4℃,老化时间为2-4h;(6) The homogeneous material liquid obtained in step (5) is cooled to 2-4°C and then aged, the aging temperature is 2-4°C, and the aging time is 2-4h;

(7)将步骤(6)中获得的成熟料液放入冰淇淋凝冻机中凝冻成型,并在-18-20℃下硬化6-24小时,获得低脂冰淇淋。(7) Put the mature liquid obtained in step (6) into an ice cream freezer to freeze and form, and harden at -18-20°C for 6-24 hours to obtain low-fat ice cream.

优选的,步骤(5)所述的高压均质压力为15-20MPa,均质温度为40-60℃,均质时间为10-15min。Preferably, the high-pressure homogenization pressure in step (5) is 15-20 MPa, the homogenization temperature is 40-60° C., and the homogenization time is 10-15 min.

本发明的一种淀粉-脂质-蛋白质复合物还可以作为食品成分用于面包、馒头和面条的制备以改善其品质。The starch-lipid-protein complex of the present invention can also be used as a food ingredient in the preparation of bread, steamed bread and noodles to improve their quality.

本发明与现有技术相比具有如下有益效果:Compared with the prior art, the present invention has the following beneficial effects:

1、本发明通过将淀粉和脂质制备成淀粉-脂质体系,然后加入蛋白质,特别是水溶性蛋白质得到淀粉-脂质-蛋白质复合物,将复合物干燥后可长期储存,这种具有独特功能性质的淀粉-脂质-蛋白质复合物能够作为食品成分应用于多种食品的制备中,其复水后作为脂肪模拟物,解决了之前利用酶/酸解淀粉制备的脂肪模拟物的析水现象,延长了保质期;使用时将样品复水并搅拌均匀后,样品无异味,且能够很好的模拟奶油制品的形状和质构,减少甚至替代冰淇淋及其他高脂食品中奶油含量,显著弥补因脂肪含量减少导致口感及风味上缺失的问题;通过调整脂质及水溶性蛋白质的种类及比例,可以得到不同质地的脂肪模拟物,减少甚至替代奶油制品,有利于实现低脂食品的工业化生产。1. The present invention prepares starch and lipids into a starch-lipid system, and then adds protein, especially water-soluble protein to obtain a starch-lipid-protein complex. The complex can be stored for a long time after being dried. The functional starch-lipid-protein complex can be used as a food ingredient in the preparation of a variety of foods. After rehydration, it can be used as a fat simulant, which solves the water separation of the fat simulant prepared by enzyme/acid hydrolyzed starch. This phenomenon has prolonged the shelf life; when the sample is rehydrated and stirred evenly during use, the sample has no peculiar smell, and can well simulate the shape and texture of cream products, reducing or even replacing the cream content in ice cream and other high-fat foods, significantly making up for The problem of lack of taste and flavor due to reduced fat content; by adjusting the types and proportions of lipids and water-soluble proteins, fat simulants with different textures can be obtained, reducing or even replacing cream products, which is conducive to the industrial production of low-fat foods .

2、本发明中淀粉-脂质-蛋白质复合物为基质的脂肪模拟物相比于之前以水解淀粉为基质脂肪模拟物具有更高的热稳定性(熔融温度>90℃),使其应用不仅仅局限于低温处理食品的制备,扩大了使用范围;相比于淀粉-脂质二元体系, 创新性的向其中加入了水溶性蛋白质,不同程度的提高了脂肪模拟物的乳化性及持水/持油性,并降低了淀粉-脂质二元体系脂肪模拟物的凝胶强度,具有更接近市售奶油冰淇淋的各项理化指标及感官性质。2. The fat simulant with starch-lipid-protein complex as the matrix of the present invention has higher thermal stability (melting temperature>90℃) than the previous fat simulant with hydrolyzed starch as the matrix, which makes its application not only It is only limited to the preparation of low-temperature processed foods, which expands the scope of use; compared to the starch-lipid binary system, water-soluble protein is innovatively added to it, which improves the emulsification and water retention of fat simulants to varying degrees /Oil retention, and reduce the gel strength of the starch-lipid binary system fat simulant, and have various physical and chemical indicators and sensory properties closer to the commercially available cream ice cream.

3、本发明中脂肪模拟物具有制备工艺简单可行,原料成本价格低廉,不易被小肠消化吸收,无需生物化学试剂,所得产品安全无毒的特点,克服了现有淀粉类脂肪模拟物的成本高、反应过程复杂以及高消化性的不足。将其应用在低脂冰淇淋的制备中,可替代普通奶油冰淇淋中50%的脂肪含量。3. The fat simulant of the present invention has the advantages of simple and feasible preparation process, low cost of raw materials, not easy to be digested and absorbed by the small intestine, no biochemical reagents, and safety and non-toxic characteristics of the obtained product, which overcomes the high cost of existing starch-based fat simulants , The reaction process is complicated and the lack of high digestibility. It can be used in the preparation of low-fat ice cream and can replace 50% of the fat content in ordinary cream ice cream.

附图说明Description of the drawings

图1为本发明淀粉-脂质-蛋白质为基质的脂肪模拟物的产品展示图;Figure 1 is a product display diagram of the fat mimic of the present invention with starch-lipid-protein as the matrix;

图2为淀粉-脂质及淀粉-脂质-蛋白质复合物为基质的脂肪模拟物的X-射线衍射图谱。Fig. 2 is an X-ray diffraction pattern of a fat mimic with starch-lipid and starch-lipid-protein complex as the matrix.

图3为淀粉-脂质及淀粉-脂质-蛋白质复合物为基质的脂肪模拟物的流变学性质对比图;Figure 3 is a comparison diagram of the rheological properties of fat mimics with starch-lipid and starch-lipid-protein complex as the matrix;

具体实施方式Detailed ways

下面结合具体实施例对本发明作进一步的说明。The present invention will be further described below in conjunction with specific embodiments.

实施例1Example 1

(1)将25g小麦淀粉与0.5g单肉豆蔻酸甘油酯和0.5gα-乳白蛋白分散在280ml水中,配置成10wt%的悬浮液;(1) Disperse 25g of wheat starch, 0.5g of glycerol monomyristate and 0.5g of α-lactalbumin in 280ml of water to prepare a 10wt% suspension;

(2)将步骤(1)得到的悬浮液充分搅拌均匀,在50℃预热1min,升温到95℃后加热2.5min,随后降温到50℃并保持2min,整个加工过程一直伴随搅拌;(2) Fully stir the suspension obtained in step (1) uniformly, preheat at 50°C for 1 min, heat up to 95°C and then heat for 2.5 min, then lower the temperature to 50°C and keep it for 2 min, the whole process is always accompanied by stirring;

(3)将步骤(2)得到的复合物样品冷冻干燥24h,搅碎后过80目筛,得到淀粉-脂质-蛋白质复合物。(3) The composite sample obtained in step (2) is freeze-dried for 24 hours, crushed and passed through an 80-mesh sieve to obtain a starch-lipid-protein complex.

本实施例的低脂冰淇淋由下述配方制成:The low-fat ice cream of this embodiment is made with the following formula:

脱脂奶粉101.2g,白砂糖120g,奶油139.3g,脂肪模拟物250g,海藻酸钠2g,单甘酯1.5g,纯净水386g。101.2g of skimmed milk powder, 120g of white sugar, 139.3g of cream, 250g of fat simulant, 2g of sodium alginate, 1.5g of monoglyceride, and 386g of purified water.

制备上述低脂冰淇淋的方法包括如下步骤:The method for preparing the aforementioned low-fat ice cream includes the following steps:

(1)向搅拌缸中加入总水量50%的水,加热至45℃后将脱脂奶粉放入搅拌缸中,充分搅拌使其溶解;(1) Add 50% of the total water to the mixing tank, heat it to 45°C, put the skimmed milk powder into the mixing tank, stir fully to dissolve it;

(2)将淀粉-脂质-蛋白质复合物复水得到脂肪模拟物,将奶油及脂肪模拟物(90%水分含量)预混后加入到搅拌缸中,充分搅拌均匀;(2) Rehydrate the starch-lipid-protein complex to obtain a fat simulant, premix the cream and the fat simulant (90% moisture content) into the mixing tank, and stir well;

(3)将白砂糖溶解在剩余的水中,向其中缓慢加入海藻酸钠和单甘酯,待充分溶解后缓慢倒入搅拌缸,充分搅拌,得到混合料液;(3) Dissolve the white granulated sugar in the remaining water, slowly add sodium alginate and monoglyceride to it, slowly pour it into the mixing tank after it is fully dissolved, and stir it fully to obtain a mixed material liquid;

(4)将步骤(3)中得到的混合料液在70℃条件下加热30min,获得灭菌料液;(4) Heating the mixed material liquid obtained in step (3) at 70° C. for 30 minutes to obtain a sterilized material liquid;

(5)将步骤(4)获得的灭菌料液进行高压均质,获得均质料液。均质压力为15-20MPa,均质温度为60℃,均质时间为15min。(5) The sterilized material liquid obtained in step (4) is subjected to high-pressure homogenization to obtain a homogeneous material liquid. The homogenization pressure is 15-20MPa, the homogenization temperature is 60℃, and the homogenization time is 15min.

(6)将步骤(5)得到的均质料液冷却到3℃后进行老化,老化温度为3℃,老化时间为4h;(6) The homogeneous material liquid obtained in step (5) is cooled to 3°C and then aged, the aging temperature is 3°C, and the aging time is 4h;

(7)将步骤(6)中获得的成熟料液放入冰淇淋凝冻机中凝冻成型,在-18℃下硬化24小时,获得低脂冰淇淋。(7) Put the mature material liquid obtained in step (6) into an ice cream freezer to freeze and form, and harden at -18°C for 24 hours to obtain a low-fat ice cream.

实施例2Example 2

(1)将25g玉米淀粉与1.25g单肉豆蔻酸甘油酯和2.5gβ-乳球蛋白分散在280ml水中,配置成10wt%的悬浮液(1) Disperse 25g of corn starch, 1.25g of glycerol monomyristate and 2.5g of β-lactoglobulin in 280ml of water to prepare a 10wt% suspension

(2)将步骤(1)得到的悬浮液充分搅拌均匀,在50℃预热1min,升温到95℃后加热2.5min,随后降温到50℃并保持2min,整个加工过程一直伴随搅拌;(2) Fully stir the suspension obtained in step (1) uniformly, preheat at 50°C for 1 min, heat up to 95°C and then heat for 2.5 min, then lower the temperature to 50°C and keep it for 2 min, the whole process is always accompanied by stirring;

(3)将步骤(2)得到的复合物样品冷冻干燥24h,搅碎后过80目筛,得到淀粉-脂质-蛋白质复合物。(3) The composite sample obtained in step (2) is freeze-dried for 24 hours, crushed and passed through an 80-mesh sieve to obtain a starch-lipid-protein complex.

本实施例的低脂冰淇淋的配方、条件和方法与实施例1相同。The formula, conditions and method of the low-fat ice cream of this embodiment are the same as those of the first embodiment.

实施例3Example 3

(1)将25g小麦淀粉与0.5g单硬脂酸甘油酯和0.625gβ-乳球蛋白分散在280ml水中,配置成10wt%的悬浮液(1) Disperse 25g of wheat starch, 0.5g of glyceryl monostearate and 0.625g of β-lactoglobulin in 280ml of water to form a 10wt% suspension

(2)将步骤(1)得到的悬浮液充分搅拌均匀,在50℃预热1min,升温到95℃后加热2.5min,随后降温到50℃并保持2min,整个加工过程一直伴随搅拌;(2) Fully stir the suspension obtained in step (1) uniformly, preheat at 50°C for 1 min, heat up to 95°C and then heat for 2.5 min, then lower the temperature to 50°C and keep it for 2 min, the whole process is always accompanied by stirring;

(3)将步骤(2)得到的复合物样品冷冻干燥24h,搅碎后过80目筛,得到淀粉-脂质-蛋白质复合物。(3) The composite sample obtained in step (2) is freeze-dried for 24 hours, crushed and passed through an 80-mesh sieve to obtain a starch-lipid-protein complex.

本实施例的低脂冰淇淋的配方、条件和方法与实施例1相同。The formula, conditions and method of the low-fat ice cream of this embodiment are the same as those of the first embodiment.

实施例4Example 4

(1)将25g马铃薯粉与1.25g单硬脂酸甘油酯和0.625gβ-乳球蛋白分散在280ml水中,配置成10wt%的悬浮液(1) Disperse 25g potato powder, 1.25g glyceryl monostearate and 0.625g β-lactoglobulin in 280ml water to prepare a 10wt% suspension

(2)将步骤(1)得到的悬浮液充分搅拌均匀,在50℃预热1min,升温到95℃后加热2.5min,随后降温到50℃并保持2min,整个加工过程一直伴随搅拌;(2) Fully stir the suspension obtained in step (1) uniformly, preheat at 50°C for 1 min, heat up to 95°C and then heat for 2.5 min, then lower the temperature to 50°C and keep it for 2 min, the whole process is always accompanied by stirring;

(3)将步骤(2)得到的复合物样品冷冻干燥24h,搅碎后过80目筛,得到将淀粉-脂质-蛋白质复合物。(3) The composite sample obtained in step (2) is freeze-dried for 24 hours, crushed, and passed through an 80-mesh sieve to obtain a starch-lipid-protein complex.

本实施例的低脂冰淇淋的配方、条件和方法与实施例1相同。The formula, conditions and method of the low-fat ice cream of this embodiment are the same as those of the first embodiment.

实施例5Example 5

(1)将25g小麦淀粉与1.25g单硬脂酸甘油酯和1.5gβ-乳球蛋白分散在280ml水中,配置成10wt%的悬浮液(1) Disperse 25g of wheat starch, 1.25g of glyceryl monostearate and 1.5g of β-lactoglobulin in 280ml of water to form a 10wt% suspension

(2)将步骤(1)得到的悬浮液充分搅拌均匀,在50℃预热1min,升温到 95℃后加热2.5min,随后降温到50℃并保持2min,整个加工过程一直伴随搅拌;(2) Fully stir the suspension obtained in step (1) evenly, preheat at 50°C for 1 min, heat up to 95°C for 2.5 min, then lower the temperature to 50°C and keep it for 2 min, the whole process is always accompanied by stirring;

(3)将步骤(2)得到的复合物样品冷冻干燥24h,搅碎后过80目筛,得到淀粉-脂质-蛋白质复合物。(3) The composite sample obtained in step (2) is freeze-dried for 24 hours, crushed and passed through an 80-mesh sieve to obtain a starch-lipid-protein complex.

本实施例的低脂冰淇淋的配方、条件和方法与实施例1相同。The formula, conditions and method of the low-fat ice cream of this embodiment are the same as those of the first embodiment.

(1)将25g小麦淀粉与1.25g月桂酸和1.5gα-乳白蛋白分散在280ml水中,配置成10wt%的悬浮液(1) Disperse 25g wheat starch, 1.25g lauric acid and 1.5g α-lactalbumin in 280ml water to form a 10wt% suspension

(2)将步骤(1)得到的悬浮液充分搅拌均匀,在50℃预热1min,升温到95℃后加热2.5min,随后降温到50℃并保持2min,整个加工过程一直伴随搅拌;(2) Fully stir the suspension obtained in step (1) uniformly, preheat at 50°C for 1 min, heat up to 95°C and then heat for 2.5 min, then lower the temperature to 50°C and keep it for 2 min, the whole process is always accompanied by stirring;

(3)将步骤(2)得到的复合物样品冷冻干燥24h,搅碎后过80目筛,得到淀粉-脂质-蛋白质复合物。(3) The composite sample obtained in step (2) is freeze-dried for 24 hours, crushed and passed through an 80-mesh sieve to obtain a starch-lipid-protein complex.

本实施例的低脂冰淇淋的配方、条件和方法与实施例1相同。The formula, conditions and method of the low-fat ice cream of this embodiment are the same as those of the first embodiment.

对比例1Comparative example 1

本对比例为制备脂肪未被替代的普通奶油冰淇淋,由下述配方制成:This comparative example is to prepare ordinary cream ice cream without replacing fat, which is made from the following formula:

脱脂奶粉101.2g,白砂糖120g,奶油282g,海藻酸钠2g,单甘酯1.5g,纯净水493g。101.2g of skimmed milk powder, 120g of white sugar, 282g of cream, 2g of sodium alginate, 1.5g of monoglyceride, and 493g of purified water.

本对比例的冰淇淋配方、条件和方法与实施例1相同。The ice cream formula, conditions and methods of this comparative example are the same as in Example 1.

对比例2Comparative example 2

本对比例为制备无奶油冰淇淋,由下述配方制成:This comparative example is to prepare cream-free ice cream, which is made from the following formula:

脱脂奶粉101.2g,白砂糖120g,海藻酸钠2g,单甘酯1.5g,纯净水775.3g。101.2g of skimmed milk powder, 120g of white sugar, 2g of sodium alginate, 1.5g of monoglyceride, and 775.3g of purified water.

本对比例的冰淇淋配方、条件和方法与实施例1相同。The ice cream formula, conditions and methods of this comparative example are the same as in Example 1.

对比例3Comparative example 3

本对比例为制备含有淀粉-脂质复合物为基质的脂肪模拟物的低脂冰淇淋。This comparative example is to prepare a low-fat ice cream containing a fat mimic of a starch-lipid complex as a matrix.

(1)将25g淀粉与1.25g单肉豆蔻酸甘油酯分散在280ml水中,配置成10wt%的悬浮液(1) Disperse 25g starch and 1.25g glycerol monomyristate in 280ml water to prepare a 10wt% suspension

(2)将步骤(1)得到的悬浮液充分搅拌均匀,在50℃预热1min,升温到95℃后加热2.5min,随后降温到50℃并保持2min,整个加工过程一直伴随搅拌;(2) Fully stir the suspension obtained in step (1) uniformly, preheat at 50°C for 1 min, heat up to 95°C and then heat for 2.5 min, then lower the temperature to 50°C and keep it for 2 min, the whole process is always accompanied by stirring;

(3)将步骤(2)得到的复合物样品冷冻干燥24h,搅碎后过80目筛,得到淀粉-脂质-蛋白质复合物。(3) The composite sample obtained in step (2) is freeze-dried for 24 hours, crushed and passed through an 80-mesh sieve to obtain a starch-lipid-protein complex.

本对比例的低脂冰淇淋的配方、条件和方法与实施例1相同。The formula, conditions and method of the low-fat ice cream of this comparative example are the same as those of Example 1.

本发明淀粉-脂质-蛋白质为基质的脂肪模拟物及利用其制备的低脂冰淇淋的相关检测结果:Related test results of the starch-lipid-protein-based fat mimic of the present invention and the low-fat ice cream prepared by using the same:

采用奥地利AntonPaar公司的MCR302流变仪测定淀粉-脂质及淀粉-脂质-蛋白质复合物为基质的脂肪模拟物的流变学性质(图2)。测定结果表明,相比于淀粉-脂质复合物,淀粉-脂质-蛋白质复合物为基质的脂肪模拟物具有更低的凝 胶强度,因此具有良好的涂抹性及可塑性。The rheological properties of starch-lipid and starch-lipid-protein complex-based fat mimics were measured using the MCR302 rheometer of AntonPaar, Austria (Figure 2). The measurement results show that compared to starch-lipid complexes, fat mimics with starch-lipid-protein complexes as the matrix have lower gel strength, and therefore have good spreadability and plasticity.

采用德国Bruker公司的X-射线衍射仪(D8-ADVANCE)检测淀粉-脂质及淀粉-脂质-蛋白质复合物为基质的脂肪模拟物的晶体结构(图3)。测定结果显示两种脂肪模拟物均展示出独特的V-型晶体衍射峰(12.9°和19.8°),表明他们主要是由具有良好酶解抗性的V-型复合物构成。The X-ray diffractometer (D8-ADVANCE) of Bruker, Germany was used to detect the crystal structure of fat mimics with starch-lipid and starch-lipid-protein complex as the matrix (Figure 3). The measurement results show that the two fat mimics both exhibit unique V-shaped crystal diffraction peaks (12.9° and 19.8°), indicating that they are mainly composed of V-shaped complexes with good enzymolysis resistance.

采用德国Netzsch公司的差示扫描量热仪(200F3)分析淀粉-脂质及淀粉-脂质-蛋白质复合物为基质的脂肪模拟物的热力学性质(表1),分析结果表明两种脂肪模拟物的熔融温度(T p)均高于90℃,具有较好的热稳定性。 The differential scanning calorimeter (200F3) of Netzsch, Germany was used to analyze the thermodynamic properties of fat simulants with starch-lipid and starch-lipid-protein complexes as the matrix (Table 1). The analysis results showed that the two fat simulants The melting temperature (T p ) is higher than 90 ℃, with good thermal stability.

对实施例1-5和对比例3中使用的脂肪模拟物进行理化性质的测定,测定结果如表2所示。The physical and chemical properties of the fat simulants used in Examples 1-5 and Comparative Example 3 were measured, and the measurement results are shown in Table 2.

脂肪模拟物乳化性测定:Determination of emulsification of fat simulants:

将1.5%(w/w)的脂肪模拟物样品分散在蒸馏水中,与5%的大豆油在室温下混合后,利用高速均质机在12000rpm下均质3min,制成均匀的乳液。取50μL乳液并与5mL 0.1%的SDS(十二烷基硫酸钠)混合均匀,以5mL 0.1%的SDS作为空白,在500nm波长下测定样品的吸光度值,所得数值可表征样品的乳化活性。A 1.5% (w/w) fat simulant sample was dispersed in distilled water, mixed with 5% soybean oil at room temperature, and homogenized using a high-speed homogenizer at 12000 rpm for 3 minutes to form a uniform emulsion. Take 50μL of emulsion and mix it with 5mL 0.1% SDS (sodium dodecyl sulfate), and use 5mL 0.1% SDS as a blank to measure the absorbance value of the sample at a wavelength of 500nm. The obtained value can characterize the emulsification activity of the sample.

脂肪模拟物持水持油性测定:Determination of water and oil retention of fat simulants:

准确称量0.5g的样品分散在5mL的蒸馏水/大豆油中,在室温下放置搅拌1h,在5000r/min下离心20min。按照下式进行计算:Accurately weigh 0.5g of the sample and disperse it in 5mL of distilled water/soybean oil, leave it at room temperature and stir for 1h, and centrifuge at 5000r/min for 20min. Calculate according to the following formula:

Figure PCTCN2020084398-appb-000001
Figure PCTCN2020084398-appb-000001

Figure PCTCN2020084398-appb-000002
Figure PCTCN2020084398-appb-000002

W2—离心管和沉淀的质量(g)W2—The mass of centrifuge tube and sediment (g)

W1—离心管和干样品的质量(g)W1—The mass of centrifuge tube and dry sample (g)

W0—样品干重(g)W0—Sample dry weight (g)

上述实验结果如表2所示,测定结果表明淀粉-脂质-蛋白质复合物为基质的脂肪模拟物相比于淀粉-脂质复合物为基质的脂肪模拟物具有更好的乳化性及持水持油性,可使食品体系更加稳定。The above-mentioned experimental results are shown in Table 2. The measurement results show that the starch-lipid-protein complex-based fat mimic has better emulsification and water retention than the starch-lipid complex-based fat mimic. Oil retention can make the food system more stable.

对实施例1-5和对比例1-3制备的冰淇淋进行理化性质的测定,测定结果如表3所示。The physical and chemical properties of the ice cream prepared in Examples 1-5 and Comparative Examples 1-3 were measured, and the measurement results are shown in Table 3.

冰淇淋表观粘度测定:将冰淇淋置于室温下融化,使用MCR302流变仪进行测定,测量转子型号为PP50(直径50mm),将冰淇淋料液滴到流变仪测试下板上,测量转子与测试下板的间隙为1mm。设定剪切速率为10rad/s进行测定。Determination of apparent viscosity of ice cream: melt the ice cream at room temperature, use MCR302 rheometer to measure, the measuring rotor model is PP50 (diameter 50mm), drop the ice cream material onto the lower test plate of the rheometer, measure the rotor and test The clearance of the lower plate is 1mm. Set the shear rate to 10rad/s for measurement.

冰淇淋硬度测定:将2.5oz纸杯中的冰淇淋样品放在-20℃下冷冻24h后立 即置于25℃环境下进行硬度测定。采用TA.XTplus质构仪P/0.5探头,测前速率1mm/s,测试速率1mm/s,测后速率10mm/s,测试深度15mm,触发应力为10g。Ice cream hardness measurement: The ice cream sample in a 2.5oz paper cup is frozen at -20°C for 24 hours and then immediately placed in an environment of 25°C for hardness measurement. Using TA.XTplus texture analyzer P/0.5 probe, the pre-test rate is 1mm/s, the test rate is 1mm/s, the post-test rate is 10mm/s, the test depth is 15mm, and the trigger stress is 10g.

冰淇淋抗融化性测定:Determination of ice cream resistance to melting:

将冰淇淋放在-20℃冰箱中硬化48h,在温度25℃,湿度50%条件下放在10目的筛网上进行融化,记录第一滴冰淇淋融化的时间。预先称好玻璃平皿的质量,之后每隔五分钟称量一次玻璃平皿的质量并进行记录,以单位时间为横坐标,平皿的质量差值为纵坐标作出时间-质量的函数,以质量变化的斜率作为冰淇淋样品的融化率。冰淇淋的抗融化性用融化率表示,融化率越低表示抗融化性越好。Place the ice cream in a refrigerator at -20°C for 48 hours and melt it on a 10-mesh sieve at a temperature of 25°C and a humidity of 50%. Record the melting time of the first drop of ice cream. Weigh the quality of the glass plate in advance, and then weigh the quality of the glass plate every five minutes and record it. The unit time is the abscissa, and the difference in the quality of the plate is the ordinate as a function of time-quality. The slope is taken as the melting rate of the ice cream sample. The melting resistance of ice cream is expressed by the melting rate, and the lower the melting rate, the better the melting resistance.

测定结果表明,实施例1-5与对比例3的X-射线衍射图谱展示出显著的V-型特征峰,表明淀粉-脂质以及淀粉-脂质-蛋白质复合物V-型有序结构的形成(图2)。同时,实施例的DSC热转变焓值都显著大于对比例3,表明淀粉-脂质-蛋白质复合物具有比淀粉-脂质复合物更有序的晶体结构;此外,所有复合物基质脂肪模拟物的热转变温度(T p)均在100℃左右,表明其具有良好的热稳定性(表1)。淀粉-脂质-蛋白质复合物相比于淀粉-脂质复合物具有更低的储能模量,因此具有更低的凝胶特性(图3)。表2数据表明淀粉-脂质-蛋白质复合物(实施例1-5)相比于对比例3具有更好的乳化性以及持水持油性。相比于淀粉-脂质复合物为基质的脂肪模拟物,使用淀粉-脂质-蛋白质为基质的脂肪模拟物制备的冰淇淋更接近于奶油冰淇淋的粘度和硬度,并且后者具有更高的抗融化特性(表3)。 The measurement results show that the X-ray diffraction patterns of Examples 1-5 and Comparative Example 3 show significant V-shaped characteristic peaks, indicating that the starch-lipid and starch-lipid-protein complexes have a V-shaped ordered structure. Formed (Figure 2). At the same time, the DSC thermal transition enthalpy values of the examples are significantly greater than that of Comparative Example 3, indicating that the starch-lipid-protein complex has a more ordered crystal structure than the starch-lipid complex; in addition, all complex matrix fat mimics The thermal transition temperature (T p ) of are all around 100°C, indicating that they have good thermal stability (Table 1). Compared with starch-lipid complexes, starch-lipid-protein complexes have a lower storage modulus and therefore have lower gel properties (Figure 3). The data in Table 2 shows that the starch-lipid-protein complex (Examples 1-5) has better emulsification and water- and oil-holding properties than Comparative Example 3. Compared with the starch-lipid complex as the matrix fat simulant, the ice cream prepared using the starch-lipid-protein matrix fat simulant is closer to the viscosity and hardness of cream ice cream, and the latter has higher resistance. Melting characteristics (Table 3).

对上述实施例1-5和对比例1-3的冰淇淋进行感官评定实验。根据感官评分表作为评分标准(表4),以对发明的以淀粉-脂质-蛋白质复合物为基质的脂肪模拟物对脂肪的替代性进行评估(表5)。Sensory evaluation experiments were performed on the ice creams of Examples 1-5 and Comparative Examples 1-3. The sensory scoring table was used as the scoring standard (Table 4) to evaluate the fat substitution of the invented fat mimic based on the starch-lipid-protein complex (Table 5).

从表5的数据可以看出,奶油冰淇淋(对比例1)具有最高的感官评价得分,而奶油的缺失导致冰淇淋的可接受度大幅下降(对比例2),利用淀粉-脂质复合物(对比例3)和淀粉-脂质-蛋白质复合物(实施例1-3)作为脂肪模拟物替代50%的奶油能够显著改善脂肪含量降低导致的冰淇淋口感及风味上缺失,特别是淀粉-脂质-蛋白质为基质的脂肪模拟物。From the data in Table 5, it can be seen that cream ice cream (Comparative Example 1) has the highest sensory evaluation score, while the lack of cream leads to a significant decrease in the acceptability of ice cream (Comparative Example 2). The use of starch-lipid complexes (Comparative Example 2) Ratio 3) and starch-lipid-protein complex (Examples 1-3) as a fat mimic instead of 50% cream can significantly improve the taste and flavor loss of ice cream caused by the decrease in fat content, especially starch-lipid- Protein is a fat mimic of the matrix.

表一脂肪模拟物的热力学性质Table 1 Thermodynamic properties of fat simulants

Figure PCTCN2020084398-appb-000003
Figure PCTCN2020084398-appb-000003

表二脂肪模拟物理化性质的测定Table 2 Determination of the physical and chemical properties of fat simulation

Figure PCTCN2020084398-appb-000004
Figure PCTCN2020084398-appb-000004

表三冰淇淋理化性质的测定Table 3 Determination of the physical and chemical properties of ice cream

Figure PCTCN2020084398-appb-000005
Figure PCTCN2020084398-appb-000005

表四冰淇淋感官质量评分标准Table 4 Ice cream sensory quality scoring standards

Figure PCTCN2020084398-appb-000006
Figure PCTCN2020084398-appb-000006

Figure PCTCN2020084398-appb-000007
Figure PCTCN2020084398-appb-000007

表五冰淇淋感官评定评分标准Table 5 Sensory Evaluation Standards for Ice Cream

Figure PCTCN2020084398-appb-000008
Figure PCTCN2020084398-appb-000008

总结上述实验结果,我们发现淀粉-脂质-蛋白质复合物为基质的脂肪模拟物具有良好的热稳定性以及独特的V-型晶体结构,且相比于淀粉-脂质复合物为基质的脂肪模拟物,具有更低的凝胶强度、更好的涂抹性、乳化性及持水持油性,将其作为食品成分加入到冰淇淋中替代脂肪可以使体系更加均一稳定,比无奶油冰淇淋及使用淀粉-脂质复合物为基质的脂肪模拟物制备的冰淇淋更接近于普通奶油冰淇淋的各种理化指标及感官性质。Summarizing the above experimental results, we found that the fat mimics with starch-lipid-protein complex as matrix have good thermal stability and unique V-shaped crystal structure, and compared with starch-lipid complex as matrix fat The simulant has lower gel strength, better spreadability, emulsification and water and oil retention. Adding it as a food ingredient to ice cream to replace fat can make the system more uniform and stable, which is more uniform and stable than cream-free ice cream and starch -The ice cream prepared by the fat mimic of the lipid complex as the matrix is closer to the various physical and chemical indexes and sensory properties of ordinary cream ice cream.

以上显示和描述了本发明的基本原理、主要特征和本发明的优点。本行业的技术人员应该了解,本发明不受上述实施例的限制,上述实施例和说明书中描述的只是说明本发明的原理,在不脱离本发明精神和范围的前提下,本发明还会有各种变化和改进,这些变化和改进都落入要求保护的本发明范围内。本发明要求保护范围由所附的权利要求书及其等效物界定。The basic principles, main features and advantages of the present invention have been shown and described above. Those skilled in the industry should understand that the present invention is not limited by the above-mentioned embodiments. The above-mentioned embodiments and descriptions only illustrate the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have Various changes and improvements, these changes and improvements all fall within the scope of the claimed invention. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims (10)

一种淀粉-脂质-蛋白质复合物的制备方法,其特征在于,包括如下步骤:A method for preparing starch-lipid-protein complex is characterized in that it comprises the following steps: (1)将淀粉和脂质混合,然后在淀粉-脂质体系中加入蛋白质,加入足量纯净水,配置成10wt%的悬浮液;(1) Mix starch and lipids, then add protein to the starch-lipid system, add sufficient purified water to prepare a 10wt% suspension; (2)将步骤(1)得到的悬浮液充分搅拌均匀,加热至淀粉凝胶化后冷却得到复合物样品;(2) Stir the suspension obtained in step (1) thoroughly, heat it until the starch is gelatinized and then cool to obtain a composite sample; (3)将步骤(2)得到的复合物样品冷冻干燥24h,搅碎后过80目筛得到淀粉-脂质-蛋白质复合物。(3) The composite sample obtained in step (2) is freeze-dried for 24 hours, crushed and passed through an 80-mesh sieve to obtain a starch-lipid-protein complex. 根据权利要求1所述的淀粉-脂质-蛋白质复合物的制备方法,其特征在于,所述步骤(1)中脂质添加量为淀粉干基重量的2-5%,蛋白质添加量为淀粉干基重量的2-10%。The method for preparing a starch-lipid-protein complex according to claim 1, wherein in the step (1), the amount of lipid added is 2-5% of the dry basis weight of starch, and the amount of protein added is starch 2-10% of dry basis weight. 根据权利要求1所述的淀粉-脂质-蛋白质复合物的制备方法,其特征在于,所述步骤(1)选用的淀粉为非蜡质淀粉,脂质为脂肪酸或单甘酯,蛋白质为水溶性蛋白质。The method for preparing a starch-lipid-protein complex according to claim 1, wherein the starch selected in the step (1) is non-waxy starch, the lipid is fatty acid or monoglyceride, and the protein is water-soluble Sex protein. 根据权利要求3所述的淀粉-脂质-蛋白质复合物,其特征在于,所述非蜡质淀粉为普通植物来源的小麦、玉米或马铃薯;所述单甘脂为单肉豆蔻酸甘油酯或单硬脂酸甘油酯;所述水溶性蛋白质为β-乳球蛋白。The starch-lipid-protein complex according to claim 3, wherein the non-waxy starch is wheat, corn or potato derived from common plants; and the monoglyceride is glycerol monomyristate or Glyceryl monostearate; the water-soluble protein is β-lactoglobulin. 根据权利要求所述的淀粉-脂质-蛋白质复合物的制备方法,其特征在于,所述步骤(2)的加工方式为:在40-50℃预热1-3min,升温到90-100℃后加热2-10min,随后降温到20-50℃并保持2-5min,整个加工过程一直伴随着搅拌。The method for preparing starch-lipid-protein complexes according to claim, characterized in that, the processing method of step (2) is: preheating at 40-50°C for 1-3 min, and heating to 90-100°C After heating for 2-10min, then cooling down to 20-50℃ and keeping it for 2-5min, the whole process is always accompanied by stirring. 一种淀粉-脂质-蛋白质复合物的应用,其特征在于,复合物复水得到脂肪模拟物,脂肪模拟物用于制备低脂冰淇淋。An application of a starch-lipid-protein complex is characterized in that the complex is rehydrated to obtain a fat mimic, and the fat mimic is used to prepare low-fat ice cream. 根据权利要求6所述的一种淀粉-脂质-蛋白质复合物的应用,其特征在于,所述冰激凌各组分质量百分比为:脱脂奶粉9-11%,白砂糖12-14%,奶油5-25%,淀粉-脂质-蛋白质复合物10-40%,海藻酸钠0.1-0.3%,单甘酯0.1-0.3%,水余量。The application of a starch-lipid-protein complex according to claim 6, wherein the mass percentage of each component of the ice cream is: 9-11% skimmed milk powder, 12-14% white sugar, 5 cream -25%, starch-lipid-protein complex 10-40%, sodium alginate 0.1-0.3%, monoglyceride 0.1-0.3%, water balance. 根据权利要求7所述的一种淀粉-脂质-蛋白质复合物的应用,其特征在于,用于制备低脂冰淇淋包括如下步骤:The use of a starch-lipid-protein complex according to claim 7, wherein the preparation of low-fat ice cream comprises the following steps: (1)向搅拌缸中加入部分水,加热至30-45℃后将脱脂奶粉放入搅拌缸中,充分搅拌使其溶解;(1) Add part of the water to the mixing tank, heat it to 30-45°C, put the skimmed milk powder into the mixing tank, and stir fully to dissolve it; (2)将淀粉-脂质-蛋白质复合物复水得到脂肪模拟物,将奶油及脂肪模拟物预混后加入到搅拌缸中,充分搅拌均匀;脂肪模拟物水分含量为90%;(2) Rehydrate the starch-lipid-protein complex to obtain a fat simulant, premix the cream and the fat simulant into the mixing tank, and stir thoroughly; the moisture content of the fat simulant is 90%; (3)将白砂糖溶解在剩余的水中,向其中缓慢加入海藻酸钠和单甘酯,待充分溶解后缓慢倒入搅拌缸,充分搅拌,得到混合料液;(3) Dissolve the white granulated sugar in the remaining water, slowly add sodium alginate and monoglyceride to it, slowly pour it into the mixing tank after it is fully dissolved, and stir it fully to obtain a mixed material liquid; (4)将步骤(3)中得到的混合料液在60-70℃条件下加热20-30min,获得 灭菌料液;(4) Heating the mixed material liquid obtained in step (3) at 60-70°C for 20-30 minutes to obtain a sterilized material liquid; (5)将步骤(4)获得的灭菌料液进行高压均质,获得均质料液;(5) Perform high-pressure homogenization of the sterilized material liquid obtained in step (4) to obtain a homogeneous material liquid; (6)将步骤(5)得到的均质料液冷却到2-4℃后进行老化,老化温度为2-4℃,老化时间为2-4h;(6) The homogeneous material liquid obtained in step (5) is cooled to 2-4°C and then aged, the aging temperature is 2-4°C, and the aging time is 2-4h; (7)将步骤(6)中获得的成熟料液放入冰淇淋凝冻机中凝冻成型,并在-18-20℃下硬化6-24小时,获得低脂冰淇淋。(7) Put the mature liquid obtained in step (6) into an ice cream freezer to freeze and form, and harden at -18-20°C for 6-24 hours to obtain low-fat ice cream. 根据权利要求8所述的一种淀粉-脂质-蛋白质复合物的应用,其特征在于,步骤(5)所述的高压均质压力为15-20MPa,均质温度为40-60℃,均质时间为10-15min。The application of a starch-lipid-protein complex according to claim 8, wherein the high-pressure homogenization pressure of step (5) is 15-20 MPa, the homogenization temperature is 40-60 ℃, and the homogenization temperature is 40-60°C. The quality time is 10-15min. 一种淀粉-脂质-蛋白质复合物的应用,其特征在于,作为食品成分用于面包、馒头和面条的制备以改善其品质。An application of a starch-lipid-protein complex is characterized by being used as a food ingredient in the preparation of bread, steamed bread and noodles to improve their quality.
PCT/CN2020/084398 2020-04-13 2020-04-13 Preparation method for starch-lipid-protein complex, and application thereof Ceased WO2021207869A1 (en)

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