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WO2021244238A1 - Puce d'essai et système d'essai - Google Patents

Puce d'essai et système d'essai Download PDF

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Publication number
WO2021244238A1
WO2021244238A1 PCT/CN2021/093087 CN2021093087W WO2021244238A1 WO 2021244238 A1 WO2021244238 A1 WO 2021244238A1 CN 2021093087 W CN2021093087 W CN 2021093087W WO 2021244238 A1 WO2021244238 A1 WO 2021244238A1
Authority
WO
WIPO (PCT)
Prior art keywords
sample
detection
channel
detection chip
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2021/093087
Other languages
English (en)
Chinese (zh)
Inventor
申晓贺
袁春根
胡立教
崔皓辰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BOE Technology Group Co Ltd
Beijing BOE Health Technology Co Ld
Original Assignee
BOE Technology Group Co Ltd
Beijing BOE Health Technology Co Ld
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BOE Technology Group Co Ltd, Beijing BOE Health Technology Co Ld filed Critical BOE Technology Group Co Ltd
Publication of WO2021244238A1 publication Critical patent/WO2021244238A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids

Definitions

  • the embodiment of the present disclosure relates to a detection chip and a detection system.
  • Microfluidic chip technology integrates the basic operation units of sample preparation, reaction, separation, and detection involved in the fields of biology, chemistry, and medicine into a chip with micrometer-scale microchannels, and automatically completes the entire process of reaction and analysis.
  • the chip used in this process is called a microfluidic chip, which can also be called a Lab-on-a-chip (Lab-on-a-chip).
  • Microfluidic chip technology has the advantages of small sample consumption, fast analysis speed, easy to make portable instruments, suitable for instant and on-site analysis, etc. It has been widely used in many fields such as biology, chemistry and medicine.
  • At least one embodiment of the present disclosure provides a detection chip, which includes a sample injection structure, a sample detection structure, and a sample filtering structure.
  • the sample injection structure is used to inject the sample to be tested
  • the sample detection structure is used to enable the tested sample to be tested
  • the sample filtering structure is between the sample injection structure and the sample detection structure and is respectively connected to the sample injection structure
  • the injected sample to be tested is filtered in a lateral chromatography manner and the filtered sample to be tested is transmitted to the sample detection structure.
  • the sample filter structure includes a filter layer configured to receive the tested sample from the sample injection structure on the first side, and The tested sample is filtered in the plane where the filter layer is located, and the filtered tested sample is output on a second side opposite to the first side.
  • the sample injection structure and the sample filtration structure are connected through a first channel, and the sample filtration structure and the sample detection structure are connected through a second channel.
  • the first channel and the filter layer are at least partially in a direction perpendicular to the plane where the filter layer is located. Overlap and connect to inject the tested sample from the sample injection structure into the filter layer for filtering.
  • the second channel and the filter layer are at least partially in a direction perpendicular to the plane where the filter layer is located. Overlap and connect to receive the tested sample after filtering.
  • the first channel and the second channel are respectively connected to different surfaces or surfaces of the filter layer. Same layer surface.
  • the sample filter structure further includes a filter cavity that accommodates the filter layer, the filter cavity includes a first opening and a second opening, and the first opening is used for Input the tested sample, the second opening is used to output the filtered tested sample, wherein the second opening is connected to the first end of the second channel, and the sample detection structure is connected to the The second end of the second channel.
  • the detection chip provided by at least one embodiment of the present disclosure further includes a first substrate and an adhesive layer, wherein the filter cavity is provided on the first substrate, and the adhesive layer is attached to the first substrate. On the surface of the substrate and fix the filter layer in the filter cavity.
  • the first channel and the second channel are provided in the first substrate.
  • the sample injection structure and the sample detection structure are disposed on the first substrate.
  • the detection chip provided by at least one embodiment of the present disclosure further includes a second substrate, wherein the second substrate is laminated with the first substrate, and is combined by the adhesive layer, and the adhesive layer defines the The first opening and the second opening of the filter cavity, the first channel and the second channel are formed in the second substrate, and respectively communicate with the first opening and the second opening .
  • the sample injection structure and the sample detection structure are disposed on the second substrate.
  • the sample detection structure includes a plurality of detection units and a plurality of first detection flow channels, and at least one of the detection units is connected to all detection units through a corresponding first detection flow channel.
  • the sample filtration structure includes a plurality of detection units and a plurality of first detection flow channels, and at least one of the detection units is connected to all detection units through a corresponding first detection flow channel.
  • the width of the first detection flow channel is 0.1 mm-1 mm, and the depth is 0.1 mm-1 mm.
  • At least part of the first detection flow channel is curved and extended.
  • At least a portion of the bent extension of the first detection flow channel is in the shape of a broken line or an S shape.
  • At least a part of the bending extension of the first detection flow channel includes 2-20 bends, and the distance between two adjacent bends is The length of the runner is 2mm-20mm.
  • the bending angle of the bending is 5°-120° .
  • the detection unit includes a detection cavity for accommodating the sample to be tested, and the detection cavity has an exhaust hole and a gas-permeable liquid barrier film covering the exhaust hole.
  • the sample injection structure includes a sampling structure mounting portion for receiving the sampling structure.
  • the sample injection structure further includes a reagent pool, and after the sampling structure is installed on the sampling structure mounting portion, the sampling structure can communicate with the reagent pool And the reagent pool is also configured to be able to communicate with the sample filtering structure.
  • the sample injection structure further includes a first sealing layer located on the first side of the reagent cell, and the first sealing layer is used to The reagent pool is sealed, and after the sampling structure is installed on the sampling structure installation part, the sampling structure can pierce the first sealing layer to communicate with the reagent pool.
  • the sample injection structure further includes a second sealing layer located on the second side of the reagent cell, and the second sealing layer is used to The reagent cell is sealed, and the first side of the reagent cell and the second side of the reagent cell are directly opposite to each other.
  • the second sealing layer is punctured, the reagent cell and the sample filtering structure Connected.
  • the sample injection structure further includes an elastic membrane and an action channel on the second side of the reagent cell, and the second sealing layer is sandwiched on the elastic membrane.
  • the action channel allows when an external force acts on the elastic membrane to deform the elastic membrane, the external force can also act on the second sealing layer to puncture the second seal Floor.
  • the material of the elastic membrane is a composite polymer material.
  • the detection chip provided by at least one embodiment of the present disclosure further includes a first substrate and an adhesive layer, wherein the sampling structure mounting portion and the reagent pool are provided on the first substrate, and the adhesive The layer is attached to the surface of the first substrate and fixes the elastic film on the first substrate.
  • the adhesive layer at least partially exposes the second sealing layer, so that the elastic film that allows deformation can act on the second sealing layer to puncture The second sealing layer.
  • the detection chip provided by at least one embodiment of the present disclosure further includes a second substrate, wherein the second substrate is laminated with the first substrate and combined with the adhesive layer, and the second substrate includes a substrate opening. To provide the action channel.
  • the detection chip provided by at least one embodiment of the present disclosure further includes a sample mixing chamber disposed between the sample injection structure and the sample filtering structure.
  • At least one embodiment of the present disclosure provides a detection system.
  • the detection chip, sampling structure, and packaging structure of any one of the foregoing detection system wherein the detection chip includes a sampling structure mounting portion, and the sampling structure is mounted on the A sampling structure mounting part, where the packaging structure is used to seal the sampling structure.
  • the packaging structure is a silicone cap.
  • the packaging structure includes a sealing part and a fixing part, the sealing part is used to seal the sampling structure, and the fixing part is used to fix the sampling structure to The detection chip.
  • the detection system provided by at least one embodiment of the present disclosure further includes a movable first ejector rod, and the first ejector rod is disposed on a side of the packaging structure away from the sampling structure.
  • the detection chip further includes a reagent pool and a second sealing layer
  • the detection system further includes a movable second ejector rod, and the second ejector rod is used for Poke the second sealing layer.
  • FIG. 1 is a perspective view of a three-dimensional structure of a detection system provided by at least one embodiment of the present disclosure
  • FIG. 2 is a perspective view of a three-dimensional structure of an upper substrate of a detection chip provided by at least one embodiment of the present disclosure
  • FIG. 3 is a three-dimensional structural diagram of a lower substrate of a detection chip provided by at least one embodiment of the present disclosure
  • FIG. 4 is an exploded top view of the detection system provided by at least one embodiment of the present disclosure.
  • Fig. 5 is a bottom exploded view of the detection system provided by at least one embodiment of the present disclosure.
  • FIG. 6 is a schematic plan view of a sample detection structure in a detection chip provided by at least one embodiment of the present disclosure
  • FIG. 7 is a perspective view of a three-dimensional structure of a sampling structure in a detection system provided by at least one embodiment of the present disclosure
  • FIG. 8A is a perspective view of a three-dimensional structure of a packaging structure in a detection system provided by at least one embodiment of the present disclosure
  • FIG. 8B is a bottom view of the packaging structure in the inspection system provided by at least one embodiment of the present disclosure.
  • FIG. 9 is a schematic diagram of a sample mixing operation performed by the detection system provided by at least one embodiment of the present disclosure.
  • FIG 10 is another schematic diagram of the sample mixing operation performed by the detection system provided by at least one embodiment of the present disclosure.
  • FIG. 11 is a schematic diagram of a flow path of a detected sample in a detection system provided by at least one embodiment of the present disclosure
  • FIG. 12 is a perspective view of a three-dimensional structure of another detection system provided by at least one embodiment of the present disclosure.
  • FIG. 13 is an exploded top view of a detection system provided by at least one embodiment of the present disclosure.
  • FIG. 14 is a schematic diagram of a flow path of a detected sample in a detection system provided by at least one embodiment of the present disclosure
  • FIG. 15 is an exploded top view of still another detection system provided by at least one embodiment of the present disclosure.
  • the sampling components, mixing components, filtering components, and analysis and detection components of the microfluidic chip can be integrated to realize the automation of the detection process.
  • the detection process of the microfluidic chip first use the sampling component to obtain the sample, and then mix the sample and the detection reagent (or diluent or other reagents that make the sample more suitable for detection) in the mixing component, and then filter it. For the next detection operation.
  • the filtration operation of the sample will improve the purity of the sample, which plays a vital role in the detection process of the microfluidic chip and the detection result.
  • At least one embodiment of the present disclosure provides a detection chip, which includes a sample injection structure, a sample detection structure, and a sample filtering structure.
  • the sample injection structure is used to inject the sample to be tested, and the sample detection structure is used to make the sample to be tested.
  • the injected test sample is filtered by lateral chromatography and the filtered test sample is transferred to the sample detection structure.
  • the detection chip of at least one embodiment of the present disclosure integrates a sample injection function, a sample detection function, and a sample filtering function, thereby enriching the functions of the detection chip, improving the integration degree of the detection chip, and helping to realize the miniaturization of the detection chip.
  • the sample filtering structure of the detection chip filters the injected sample to be detected in the manner of lateral chromatography, which further contributes to the realization of the thinning of the overall shape of the detection chip.
  • the miniaturization and thinning of the detection chip contributes to the realization of a portable detection and analysis device.
  • At least one embodiment of the present disclosure provides a detection system with the above-mentioned detection chip, sampling structure and packaging structure.
  • the detection chip includes a sampling structure mounting part, the sampling structure is mounted on the sampling structure mounting part, and the packaging structure is used for sealing the sampling structure.
  • At least one embodiment of the present disclosure provides a detection chip and a detection system.
  • the detection system includes the detection chip and components used in conjunction with the detection chip.
  • Figure 1 shows a perspective view of the three-dimensional structure of the detection system provided by this embodiment
  • Figure 2 shows a perspective view of the three-dimensional structure of the upper substrate of the detection chip provided by this embodiment
  • Figure 3 shows a perspective view of the three-dimensional structure provided by this embodiment.
  • the three-dimensional structure diagram of the lower substrate of the detection chip FIG. 4 shows a top exploded view of the detection system
  • FIG. 5 shows a bottom exploded view of the detection system provided by this embodiment.
  • the detection chip 100 includes a sample injection structure 101, a sample detection structure 102 and a sample filtering structure 103.
  • the sample injection structure 101 is used to inject a sample to be tested.
  • the sample detection structure 102 is used to enable the sample to be detected to be detected.
  • the sample detection structure 102 has a detection reagent, and the detection operation is performed after the sample to be detected is mixed with the detection reagent.
  • the detection reagent is a freeze-dried reagent, and the sample to be tested can be re-thawed with the freeze-dried reagent, and the required reaction occurs, so as to be suitable for subsequent detection operations. These detection operations can be optical detection as required.
  • the sample filtering structure 103 is between the sample injection structure 101 and the sample detection structure 102 and communicates with the sample injection structure 101 and the sample detection structure 102 respectively, so as to filter the injected test sample in a lateral chromatography manner and filter the sample.
  • the subsequent sample to be tested is transferred to the sample testing structure 102.
  • the filtering method of lateral chromatography can make the tested sample be fully and uniformly filtered, which can make the tested sample after filtration more pure and improve the detection effect.
  • the space used by the filtering method of lateral chromatography is reduced. It has a thin layer shape, which contributes to the thinning of the detection chip 100.
  • the sample filter structure 103 includes a filter layer 1031. As shown in FIG. The test sample is filtered in the plane, and the filtered test sample is output on the second side 1031B opposite to the first side 1031A.
  • the sample to be tested enters the filter layer 1031 from the first side of the filter layer 1031, passes through the filter layer 1031 laterally, and is output from the second side of the filter layer 1031, because the filter path of the sample to be tested is the lateral dimension of the filter layer 1031 ( The length or width) is greater than or far greater than the thickness of the filter layer 1031, so that the sample to be tested can be sufficiently filtered. For example, when the amount of the tested sample is large, since the filtering path of the tested sample is basically the same, the tested sample can also be sufficiently and uniformly filtered.
  • the sample injection structure 101 and the sample filtering structure 103 are connected through a first channel 104, and the sample filtering structure 103 and the sample detection structure 102 are connected through a second channel 105; or, in another
  • the sample injection structure 101 and the sample filtration structure 103 may be directly connected, and the sample filtration structure 103 and the sample detection structure 102 may be directly connected.
  • one end of the first channel 104 connected to the sample filter structure 103 includes the first channel cavity 1041, so that the tested sample can be evenly input into the sample filter structure 103, and avoid the tested sample in the first channel.
  • the connection between the channel 104 and the filtering structure 103 is concentrated.
  • the first channel cavity 1041 has a circular shape, an oval shape, or a drop shape (the case shown in the figure).
  • the first channel 104 and the filter layer 1031 at least partially overlap and meet in a direction perpendicular to the plane of the filter layer 1031 to inject the sample from the structure 101
  • the sample to be tested is injected into the filter layer 1031 for filtering, so the contact area between the first channel 104 and the filter layer 1031 is relatively large, which makes it easier to inject the test sample into the filter layer 1031.
  • the first channel 104 when the upper substrate and the lower substrate of the detection chip are combined, at least part of the first channel 104, such as the first channel cavity 1041, and the filter layer 1031 are perpendicular to the plane where the filter layer 1031 is located. Overlap in the direction of each other.
  • the first channel cavity 1041 extends above the filter layer 1031 so as to overlap with the filter layer 1031.
  • the first channel 104 can inject the tested sample into the filter layer 1031 along a direction perpendicular to the plane where the filter layer 1031 is located, so that the tested sample can be evenly dispersed before entering the filter layer 1031 to avoid filtering caused by sample concentration. not effectively.
  • the overlapping area of the first channel cavity 1041 and the filter layer 1031 is relatively large, which facilitates the dispersion of the tested sample and prevents the tested sample from being concentrated at the connection position of the filter layer 1031 and the first channel 104. Therefore, the above-mentioned filtering structure can allow the tested sample to be fully and uniformly filtered, and thereby make the filtered tested sample more pure.
  • the second channel 105 and the filter layer 1031 at least partially overlap and meet in a direction perpendicular to the plane of the filter layer 1031 to receive the filtered sample to be tested.
  • at least part of the second channel 105 extends above or below the filter layer 1031 so as to overlap with the filter layer 1031.
  • the second channel 105 can output the sample to be tested from the filter layer 1031 in a direction perpendicular to the plane where the filter layer 1031 is located.
  • the first channel 104 and the second channel 105 are respectively connected to the surface of different layers of the filter layer 1031 (that is, the length direction and the width direction of the filter layer define The lateral surface) or the same layer surface.
  • the layer surface mentioned in the embodiment of the present disclosure refers to the surface of the filter layer 1031 parallel to the plane where the filter layer 1031 is located, that is, the upper surface and the lower surface of the filter layer 1031 shown in the figure.
  • the first channel 104 and the second channel 105 are respectively connected to the upper surface and the lower surface of the filter layer 1031, and may also be connected to the upper surface or the lower surface of the filter layer 1031 at the same time.
  • the first channel 104 may be connected to the layer surface of the filter layer 1031
  • the second channel 105 may be connected to the side surface of the filter layer 1031, which is between the upper surface and the lower surface of the filter layer 1031. s surface.
  • the embodiment of the present disclosure does not limit the specific connection manner of the first channel 104 and the second channel 105, as long as the first channel 104 can inject the tested sample into the filter layer 1031 in a direction perpendicular to the plane where the filter layer 1031 is located.
  • the sample filter structure 103 further includes a filter cavity 1032 that accommodates the filter layer 1031.
  • the filter cavity 1032 includes a first opening 1032A and a second opening 1032B.
  • the first opening 1032A is used to input the tested sample
  • the second opening 1032B is used to output the filtered tested sample.
  • the second opening 1032B is connected to the first end of the second channel 105 (for example, the right end shown in the figure), and the sample detection structure 102 is connected to the second end of the second channel 105 (for example, the left end shown in the figure).
  • the detection chip further includes a first substrate and an adhesive layer, the filter cavity 1032 is provided on the first substrate, and the adhesive layer is attached to the surface of the first substrate and the filter layer 1031 is fixed on the surface of the first substrate.
  • the first substrate can also be used to form/support other functional structures.
  • the detection chip further includes a second substrate, the second substrate is laminated with the first substrate, and is combined by an adhesive layer, and the second substrate can also form/support other functional structures, or the first substrate and the second substrate share the same Form/support other functional structures.
  • the detection chip includes an upper substrate 100A and a lower substrate 100B
  • the lower substrate 100B is implemented as an example of the above-mentioned first substrate
  • the upper substrate 100A is implemented as an example of the above-mentioned second substrate.
  • the upper substrate 100A and the lower substrate 100B are combined by an adhesive layer 100C.
  • the material of the upper substrate 100A may be polystyrene (PS) or polymethyl methacrylate (PMMA)
  • the material of the lower substrate 100B may also be polystyrene (PS) or polymethyl methacrylate (PMMA). )Wait.
  • the adhesive layer 100C may include an adhesive material such as an acrylic adhesive, for example, may be implemented as a double-sided tape.
  • an adhesive material such as an acrylic adhesive
  • the upper substrate 100A, the adhesive layer 100C, and the lower substrate 100B have substantially the same shape, so that the adhesive layer 100C can better realize the bonding between the upper substrate 100A and the lower substrate 100B.
  • the adhesive layer 100C defines the first opening 1032A and the second opening 1032B of the filter cavity 1032.
  • the adhesive layer 100C has the first opening and the second opening, and the adhesive layer 100C is attached to the surface of the lower substrate 100B to filter
  • the layer 1031 is fixed in the filter cavity 1032 so that the first opening and the second opening of the adhesive layer 100C are formed as the first opening 1032A and the second opening 1032B of the filter cavity 1032. Therefore, the adhesive layer 100C not only plays the role of bonding, but also plays the role of sealing the filter cavity 1032, and provides a flow channel for the sample to be tested through the first opening and the second opening, so as to facilitate the realization of the sample filtering structure 103
  • the lateral chromatography filtration function is the adhesive layer 100C.
  • the first channel 104 and the second channel 105 may both be formed in the upper substrate 100A and communicate with the first opening 1032A and the second opening 1032B, respectively.
  • the sample injection structure 101 and the sample detection structure 102 are also provided on the upper substrate 100A.
  • the sample injection structure 101 located on the upper substrate 100A can pass the detected sample through the first opening 1032A in a direction perpendicular to the plane where the filter membrane 1031 is located.
  • the filtered sample to be tested is output to the sample detection structure 102 on the upper substrate 100A through the second opening 1032B in a direction perpendicular to the plane of the filter membrane 1031. In this way, the functions of lateral chromatography and vertical liquid feeding of the sample filtering structure 103 are realized.
  • the sample detection structure 102 includes a plurality of detection units 1021 and a plurality of first detection flow channels 1022, and at least one detection unit 1021 is connected to the sample filtering structure 103 through a corresponding first detection flow channel 1022.
  • the detection unit 1021 is connected to the second end of the second channel 105 (for example, the left end of the second channel 105 shown in the figure) through the corresponding first detection flow channel 1022, so as to be filtered by the sample filtering structure 103.
  • the detection sample can flow into the detection unit 1021 from the second channel 105 and the first detection flow channel 1022.
  • each detection unit 1021 is connected to the second end of the second channel 105 through a corresponding first detection flow channel 1022.
  • the second end of the second channel 105 has an auxiliary connection structure 1051, and the extension direction of the auxiliary connection structure 1051 is perpendicular to the extension direction of the second channel 105.
  • the first detection flow passages 1022 are all connected to the second end of the second passage 105 through the auxiliary connection structure 1051, thereby facilitating the arrangement of the first detection flow passages 1022 and making the length of each first detection flow passage 1022 Basically the same as the shape.
  • the width W of the first detection flow channel 1022 may be 0.1 mm-1 mm, such as 0.3 mm, 0.5 mm, or 0.7 mm.
  • the depth of the first detection flow channel 1022 ( That is, the size in the direction perpendicular to the plane where the upper substrate 100A is located) may be 0.1 mm-1 mm, such as 0.3 mm, 0.5 mm, or 0.7 mm. Therefore, the sample to be detected can have a suitable flow rate and flow rate in the first detection flow channel 1022.
  • At least part of the first detection flow channel 1022 is curved and extended.
  • at least a part of the bending extension of the first detection flow channel 1022 is in the shape of a broken line or an S shape.
  • at least part of the bending extension of the first detection flow channel 1022 may include 2-20 bends 1022A, such as 5, 8, 12, 16, etc., between two adjacent bends 1022A.
  • the length L of the runner can be 2mm-20mm, such as 3mm, 5mm, 10mm, or 15mm.
  • the bending angle ⁇ of the bending 1022A may be 5°-120°, such as 30°, 60°, or 90° and so on.
  • the bent and extended part can extend the flow channel of the tested sample; on the other hand, the bent The bend 1022A of the extended part can also increase the flow resistance of the tested sample, so that the tested sample is not easy to flow back, thereby preventing crosstalk of the tested sample in the multiple detection units 1021, thereby preventing the crosstalk of the tested sample from occurring.
  • the detection error caused by the design of the first detection flow channel 1022 can improve the detection accuracy and detection quality.
  • the detection unit 1021 includes a detection cavity (such as the columnar structure shown in the figure) containing the sample to be detected, and the detection cavity has an exhaust hole 1021A and a gas-permeable liquid barrier film 1021B covering the exhaust hole 1021A.
  • the detection cavity such as the columnar structure shown in the figure
  • the detection cavity has an exhaust hole 1021A and a gas-permeable liquid barrier film 1021B covering the exhaust hole 1021A.
  • the vent 1021A can exhaust the excess air in the detection chamber to balance the air pressure.
  • the breathable liquid barrier film 1021B has the function of breathable but liquid impermeable This can prevent the sample to be tested from flowing out of the detection chamber.
  • the vent 1021A may be formed on the side of the upper substrate 100A (the left end surface of the upper substrate 100A shown in FIG. 2 or FIG. 4), and the air-permeable and liquid-resistant film 1021B may be It is pasted on the side surface of the upper substrate 100A, thereby covering the exhaust hole 1021A.
  • the air-permeable and liquid-resistant membrane 1021B covering the exhaust hole 1021A of the plurality of detection units 1021 is an integrated structure.
  • the one-piece air-permeable liquid barrier film 1021B can cover the entire surface of the multiple detection units 1021 on the side with the exhaust holes 1021A (as shown in Figures 4 and 5), which can simplify the detection chip Structure and difficulty of production.
  • the breathable liquid barrier film 1021B may be an ePTFE (expanded polytetrafluoroethylene) breathable liquid barrier film, which is not limited in the embodiments of the present disclosure.
  • the sample injection structure 101 includes a sampling structure mounting portion 1011, and the sampling structure mounting portion 1011 is used to receive a sampling structure, for example, a sampling structure is installed, and the sampling structure contains a sample to be tested.
  • the sample injection structure 101 also includes a reagent pool 1012.
  • the reagent pool 1012 is used to store test reagents or diluents and other reagents that make the sample more suitable for testing. As shown in FIG. 4, the reagent pool 1012 is formed in the upper substrate 100A.
  • the sampling structure After the sampling structure is installed in the sampling structure mounting part 1011, the sampling structure can be connected with the reagent tank 1012, for example, the sampling structure is further pressed to communicate with the reagent tank 1012, and the reagent tank 1012 can also be connected with the sample filtering structure 103.
  • the mixing ratio of the tested sample and the diluent is determined.
  • the volume of the diluent in the reagent tank 1012 can be selected and adjusted to facilitate obtaining the desired mixing ratio.
  • the total amount of samples obtained by the sampling structure is known (for example, the total amount of samples obtained by the sampling structure is a fixed value or can be read), so you can choose to select the volume of the diluent in the reagent tank 1012 to control the Detect the mixing ratio of the sample and the diluent, realize the quantification of the sample, and obtain a sample with a certain concentration, etc.
  • the sample injection structure 101 may further include a first sealing layer 1013 located on a first side (upper side shown in the figure) of the reagent cell 1012, and the first sealing layer 1013 is used to seal the reagent cell 1012 on the first side.
  • the sampling structure After the sampling structure is installed in the sampling structure mounting portion 1011, the sampling structure can be pressed to puncture the first sealing layer 1013 to communicate with the reagent tank 1012.
  • the sample injection structure 101 may further include a second sealing layer 1014 located on the second side of the reagent cell 1012 (shown as the lower side in the figure).
  • the second sealing layer 1014 is used to seal the reagent cell 1012 on the second side.
  • the first side of the cell 1012 and the second side of the reagent cell 1012 are directly opposite to each other. After the second sealing layer 1014 is punctured, the reagent tank 1012 can be connected to the sample filtering structure 103.
  • the first sealing layer 1013 and/or the second sealing layer 1014 can be aluminum foil.
  • the first sealing layer 1013 and/or the second sealing layer 1014 can be formed on both sides of the reagent cell 1012 by heat sealing, respectively. , Thus forming a sealed reagent storage space.
  • the first sealing layer 1013 and/or the second sealing layer 1014 can also be made of other materials with sealing function, such as polymethylmethacrylate (PMMA), polypropylene (PP), etc.
  • a sealing layer 1013 and/or a second sealing layer 1014 can be respectively formed on both sides of the reagent pool 1012 by means of ultrasonic welding, thereby forming a sealed reagent storage space.
  • the embodiment of the present disclosure does not limit the specific form of the first sealing layer 1013 and/or the second sealing layer 1014.
  • the sample injection structure 101 may further include an elastic membrane 1015 and an action channel 1016 on the second side of the reagent cell 1012, and the second sealing layer 1014 is sandwiched between the elastic membrane 1015 and the reagent cell 1012.
  • the adhesive layer 100C is sandwiched between the elastic film 1015 and the second sealing layer 1014, thereby bonding and fixing the elastic film 1015 and the second sealing layer 1014 to each other.
  • the adhesive layer 100C also has a third opening 1001, and the third opening 1001 is also located in the area where the elastic membrane 1015 and the second sealing layer 1014 overlap each other directly,
  • the third opening 1001 is completely located in the area where the elastic film 1015 and the second sealing layer 1014 are overlapped with each other, and the area where the elastic film 1015 and the second sealing layer 1014 are overlapped with each other is completely bonded by the adhesive layer 100C fixed.
  • the acting channel 1016 allows when an external force acts on the elastic membrane 1015 to deform the elastic membrane 1015, the deformed elastic membrane 1015 partly passes through the third opening 1001 of the adhesive layer 100C, thereby in the case where the elastic membrane 1015 itself is not punctured
  • the external force can also act on the second sealing layer 1014 to puncture the second sealing layer 1014.
  • the elastic membrane 1015 will basically return to its original shape.
  • the material of the elastic film 1015 is a composite polymer material, such as a composite material of polystyrene (PS) and polyethylene terephthalate (PET). Therefore, the elastic film 1015 can have good elasticity and strength at the same time.
  • the elastic film 1015 is bonded between the upper substrate and the lower substrate, for example, by an adhesive layer 100C.
  • the elastic film 1015 can also be combined between the upper substrate and the lower substrate by ultrasonic welding, photosensitive adhesive bonding, chemical solvent bonding, or laser welding, etc. The embodiments of the present disclosure do not do this. limited.
  • the adhesive layer 100C at least partially exposes the second sealing layer 1014 through the third opening 1001, so that the deformed elastic film 1015 can act on the second sealing layer 1014 to pierce the second sealing layer 1014.
  • the lower substrate 100B includes a substrate opening to provide an action channel 1016.
  • the elastic film 1015 completely covers the action channel 1016, and the projection of the third opening 1001 of the adhesive layer 100C on the lower substrate 100B at least partially overlaps the action channel 1016, for example, The effect channel 1016 is completely covered.
  • the detection system provided by this embodiment includes a sampling structure 110 and a packaging structure 111 in addition to the above-mentioned detection chip.
  • the sampling structure 110 is installed in the sampling structure mounting portion 1011 of the sample injection structure 101, and the packaging structure 111 is used to seal the sampling structure 110.
  • the sampling structure 110 may be installed on the sampling structure mounting portion 1011 through a gasket 140, the gasket 140 is, for example, a silicone material, which can play a role of sealing and buffering.
  • the sampling structure 110 may be a sampling needle with sample suction and mixing functions.
  • the packaging structure 111 may be a silicone cap.
  • the sampling structure 110 and the packaging structure 111 can cooperate with each other to complete the sample mixing function.
  • FIG. 9 shows a schematic cross-sectional view of the sample injection structure 101 when the sampling structure 110 is installed on the sampling structure mounting portion 1011 and the packaging structure 111 seals the sampling structure 110.
  • the sampling structure 110 includes a first suction channel 110A, a second suction channel 110B, and a chamber 110C.
  • a plurality of partition columns 110D are provided in the second suction channel 110B.
  • the first suction channel 110A and the second suction channel 110B of the sampling structure are configured to suck the sample to be tested (for example, blood, body fluid, etc.) by capillary action, and the separation column 110D provided in the second suction channel 110B can flow through the second The sample to be tested in the suction channel 110B is split.
  • the sampling structure can play a role of sample mixing.
  • the packaging structure 111 includes a main body portion 111A and at least one vent hole 111B provided at a peripheral position of the main body portion 111A, such as a plurality of vent holes 111B.
  • these vent holes 111B are evenly distributed around the main body portion 111A.
  • the exhaust hole 110B is configured to be in an open state or a closed state when the main body 111A receives different forces.
  • the vent hole 110B may be a triangular prism vent hole.
  • the vent hole 110B is closed.
  • the exhaust hole 110B is in an open state. Therefore, the internal pressure of the sampling structure 110 can be adjusted by applying different forces to the main body portion 111A of the packaging structure 111.
  • the detection system further includes a movable first jack 120, and the first jack 120 is disposed on a side of the packaging structure 111 away from the sampling structure 110.
  • the first jack 120 can be moved up and down on the main body portion 111A of the packaging structure 111 to apply a force to the main body portion 111A.
  • the force applied by the first ejector rod 120 to the main body 111A is adjustable, so that the pressure in the sampling structure 110 can be controlled to be small.
  • a driving device such as a stepping motor
  • the main body portion 111A may include a recessed groove for guiding the force application position of the first jack 120.
  • the concave trough has the same shape as the first ejector rod 120, for example, the cross section thereof is both circular.
  • the diameter of the recessed platform groove is slightly larger than the diameter of the first jack 120, and has a step, so as to facilitate the guidance of the force application position of the first jack 120.
  • the detection system may further include a movable second mandrel 130, and the second mandrel 130 is used to pierce the second sealing layer 1014.
  • the second mandrel 130 applies a force to the elastic membrane 1015 through the acting channel 1016, so that the deformed elastic membrane 1015 can act on the second sealing layer 1014 to puncture the second sealing layer 1014.
  • another driving device for example, a stepping motor
  • the sample to be tested is sucked by the sampling structure 110.
  • the sample to be tested can be sucked from the first suction channel 110A and the second suction channel 110B of the sampling structure 110 by capillary action.
  • the sample to be detected may be, for example, blood, body fluid, etc., which is not limited in the embodiments of the present disclosure.
  • the sampling structure 110 is mounted on the detection chip 100, and the packaging structure 111 is used to fix and seal the sampling structure 110.
  • the bottom of the sampling structure 110 extends into the reagent pool 1012 of the detection chip 100.
  • the upper surface of the reagent pool 1012 has a first sealing layer 1013 for sealing.
  • the sampling structure 110 can pierce the first sealing layer 1014, thereby connecting with the reagent pool 1012 It is connected so that the sample to be tested can be mixed with the diluent in the reagent tank 1012.
  • two connected sealed cavities are formed in the detection chip 100, one is the first sealed cavity formed by the sampling structure 110, and the other is the second sealed cavity formed by the reagent tank 1012.
  • the first ejector rod 120 is used to apply a force to the main body portion 111A of the sealing structure 111 at a first speed.
  • the exhaust hole 111B of the sealing structure 111 is in an open state ,
  • the sampling structure 110 can exhaust air or overflow through the exhaust hole 111B.
  • the applied force gradually increases to greater than or equal to the threshold pressure, the exhaust hole 111B is in a closed state.
  • the pressure inside the sampling structure 110 increases, so that the tested sample is pushed out of the sampling structure 110 and enters the reagent In the pool 1012, as shown by the arrow in FIG. 9.
  • the sample to be tested can be mixed with the diluent in the reagent cell 1012.
  • the first ejector rod 120 is withdrawn at the second speed.
  • the main body 111A rebounds, and the mixed solution of the diluent and the tested sample in the reagent tank 1012 will be sucked back into the sampling structure 110.
  • the second suction channel 110B has a separation column 110D, and a plurality of narrow gaps are formed between the adjacent separation columns 110D and between the separation column 110D and the channel wall, the diluent and the detected
  • the sample mixture passes through the first suction channel 110A and the second suction channel 110B, its flow rate will increase, and then the mixture can rush into the chamber 110C of the sampling structure 110 at a relatively high speed, and interact with the sampling structure 110.
  • the test sample forms a convoluted mixture, as shown by the arrow in Figure 10, which can improve the mixing efficiency and make the diluent and the test sample mix more evenly. At this point, one mixing operation is completed.
  • the first speed at which the first jack 120 exerts a force on the main body portion 111A of the sealing structure 111 is greater than the second speed at which the first jack 120 withdraws, that is, the first jack 120 is quickly pressed down and slowly raised. This operation helps to improve the mixing effect of the sample to be tested and the diluent in the sampling structure 110.
  • the above mixing operation can be performed multiple times to further improve the mixing effect of the tested sample and the diluent.
  • the second ejector 130 can be driven to move upward, so that the second ejector 130 passes through the action channel 1016 and the elastic membrane 1015 to the second sealing layer 1014 below the reagent cell 1012 A force is applied to puncture the second sealing layer 1014. Since the elastic membrane 1015 is elastic, it can be restored to its original state after the external force is removed. After the second sealing layer 1014 is punctured, the reagent tank 1012 is connected to the sample filtering structure 103.
  • Figure 11 shows the flow path of the sample being tested in more detail.
  • the first ejector rod 120 can continue to be pressed slowly and continuously, so that the tested sample enters the sample filter structure 103 (the tested sample is input into the filter membrane 1031 in a direction perpendicular to the plane of the filter membrane 1031, for example) ,
  • the filtered sample to be tested can be transported to the sample detection structure 102 during the continuous pressing of the first ejector rod 120 (the sample to be tested is output to the sample detection structure 102 in a direction perpendicular to the plane of the filter membrane 1031, for example) , For example, transported to the detection cavity 1021 of the sample detection structure 102.
  • the detection cavity 1021 contains freeze-dried reagents suitable for different detection items, so that after the sample to be tested can react with the freeze-dried reagent, the sample detection structure 102 starts to perform detection and outputs the detection result.
  • the first ejector rod 120 is always kept in a depressed state to avoid the backflow of the tested sample.
  • the detection system can realize an automated detection process, and the detection system can also obtain more accurate detection results.
  • the detection chip 100 may further include a sample mixing chamber (not shown in the figure) disposed between the sample injection structure 101 and the sample filtering structure 103.
  • a sample mixing chamber (not shown in the figure) disposed between the sample injection structure 101 and the sample filtering structure 103.
  • the tested sample and the diluent can also be mixed in the sample mixing chamber.
  • the mixing operation may be completed by the sampling structure 110 and the sample mixing chamber together, or, in some embodiments, the sampling structure 110 may only have a sampling function, so that the mixing operation can be performed only in the sample mixing chamber. The embodiment does not limit this.
  • At least one embodiment of the present disclosure provides another detection chip and detection system, and the detection system includes the detection chip.
  • FIG. 12 shows a perspective view of the three-dimensional structure of the detection system provided by this embodiment
  • FIG. 13 shows an exploded top view of the detection system.
  • the detection chip 200 includes a sample injection structure 201, a sample detection structure 202 and a sample filtering structure 203.
  • the sample injection structure 201 is used to inject the sample to be tested
  • the sample detection structure 202 is used to enable the tested sample to be tested
  • the sample filtering structure 203 is between the sample injection structure 201 and the sample detection structure 20 and is connected to the sample injection structure 201 and the sample respectively.
  • the detection structure 202 is connected to filter the injected test sample in a lateral chromatographic manner and transmit the filtered test sample to the sample detection structure 202.
  • the sample filtering structure 203 includes a filter layer 2031 configured to receive the tested sample from the sample injection structure 201 on the first side 2031A, and filter the tested sample in a plane along the filter layer 2031.
  • the filtered sample is output on the second side 2031B opposite to the first side 2031A, so that the filter layer 2031 can realize the lateral chromatography filtering function.
  • sample injection structure 201 and the sample filtering structure 203 are connected through a first channel 204, and the sample filtering structure 203 and the sample detection structure 202 are connected through a second channel 205.
  • the first channel 204 and the filter layer 2031 at least partially overlap in a direction perpendicular to the plane of the filter layer 2031, so as to inject the tested sample from the sample injection structure into the filter layer. 2031 for filtering.
  • the first channel 204 overlaps and connects with the filter layer 2031 on the side and above the filter layer 2031, so that the sample to be tested can be input to the filter layer 2031 in a lateral direction and a direction perpendicular to the plane of the filter layer 2031 at the same time. sample.
  • the sample to be tested can be evenly dispersed before entering the filter layer 2031, so as to avoid poor filter effect caused by sample concentration.
  • one end of the first channel connected to the sample filtering structure 203 may also have a first channel cavity 2041.
  • the first channel cavity 2041 is overlapped and connected with the filter layer 2031 above and on the side of the filter layer 2031.
  • the first channel cavity 2041 has, for example, a circular shape, an oval shape, or a drop shape (the case shown in FIG. 12). Since the overlapping area of the first channel cavity 2041 and the filter layer 2031 is relatively large, it is beneficial to the dispersion of the tested sample, and prevents the tested sample from being concentrated at the connection position of the filter layer 2031 and the first channel 204. Therefore, the above-mentioned filtering structure can allow the tested sample to be fully and uniformly filtered, thereby making the tested sample more pure.
  • the sample filter structure 203 further includes a filter cavity 2032 containing the filter layer 2031, and the filter cavity 2032 includes a first opening 2032A and a second opening 2032B.
  • the first opening 2032A is used to input the tested sample
  • the second opening 2032B is used to output the filtered tested sample.
  • the second opening 2032B is connected to the first end of the second channel 205
  • the sample detection structure 202 is connected to the second end of the second channel 205.
  • the detection chip further includes a first substrate and an adhesive layer, the filter cavity 2032 is disposed on the first substrate, and the adhesive layer is attached to the surface of the first substrate and fixes the filter layer 2031 in the filter cavity 2032.
  • the detection chip further includes a second substrate, and the second substrate is laminated with the first substrate and is combined by an adhesive layer.
  • the detection chip includes an upper substrate 200A and a lower substrate 200B
  • the upper substrate 200A is implemented as an example of the above-mentioned first substrate
  • the lower substrate 200B is implemented as an example of the above-mentioned second substrate
  • the upper substrate 200A and the lower substrate 200B Bonded by the adhesive layer 200C.
  • the upper substrate 200A, the adhesive layer 200C, and the lower substrate 200B have substantially the same shape, so that the adhesive layer 200C can better realize the bonding between the upper substrate 200A and the lower substrate 200B.
  • the materials of the upper substrate 200A, the lower substrate 200B, and the adhesive layer 200C can be referred to the above-mentioned embodiments, which will not be repeated here.
  • the first channel 204 and the second channel 205 are provided in the upper substrate 200A.
  • the sample injection structure 201 and the sample detection structure 202 are also provided in the upper substrate 200A.
  • the sample injection structure 101 can input the tested sample into the sample filter structure 203 from the side and perpendicular to the plane of the filter membrane 2031 through the first opening 2032A, and transfer the filtered test sample from the side , Output to the sample detection structure 102 through the second opening 1032B. In this way, the functions of lateral chromatography and vertical liquid feeding of the sample filtering structure 103 are realized.
  • the sample detection structure 202 includes a plurality of detection units 2021 and a plurality of first detection flow channels 2022, and at least one detection unit 2021 is connected to the sample filtering structure 203 through a corresponding first detection flow channel 2022.
  • the structure, size parameters, and connection mode of the first detection flow channel 2022 can be referred to the foregoing embodiment, and will not be repeated here.
  • the detection unit 2021 includes a detection cavity for accommodating a sample to be tested, and the detection cavity has an exhaust hole 2021A and a gas-permeable liquid barrier film 2021B covering the exhaust hole 2021A.
  • the gas-permeable liquid-blocking membrane 2021B has the function of gas-permeability but liquid-impermeability, so as to prevent the sample to be tested from flowing out of the detection cavity.
  • the air-permeable liquid barrier film 2021B covering the exhaust hole 2021A of the multiple detection units 2021 is an integrated structure.
  • the side of the detection unit 2021 with the exhaust hole 2021A (as shown in FIG. 13) can simplify the structure and manufacturing difficulty of the detection chip.
  • the sample injection structure 201 includes a sampling structure mounting portion 2011 for receiving a sampling structure, for example, installing a sampling structure.
  • the sample injection structure 201 further includes a reagent tank 2012. After the sampling structure is installed in the sampling structure mounting part 2011, the sampling structure may be connected to the reagent tank 2012, and the reagent tank 2012 may also be connected to the sample filtering structure 203.
  • the sample injection structure 201 may further include a first sealing layer 2013 located on the first side of the reagent cell 2012 (shown as the upper side in the figure), and the first sealing layer 2013 is used to Seal the reagent cell 2012.
  • the sampling structure After the sampling structure is installed in the sampling structure installation part 2011, the sampling structure can pierce the first sealing layer 2013 to communicate with the reagent tank 2012.
  • the sample injection structure 201 may further include a second sealing layer 2014 located on the second side of the reagent cell 2012 (shown as the lower side in the figure), the second sealing layer 2014 is used to seal the reagent cell 2012 on the second side, and The first side of the reagent pool 2012 and the second side of the reagent pool 2012 are directly opposite to each other. After the second sealing layer 2014 is punctured, the reagent tank 2012 is in communication with the sample filtering structure 203.
  • the sample injection structure 201 may further include an elastic membrane 2015 and an action channel 2016 located on the second side of the reagent cell 2012, and the second sealing layer 2014 is sandwiched between the elastic membrane 2015 and the reagent cell 2012.
  • the adhesive layer 100C is sandwiched between the elastic film 2015 and the second sealing layer 2014, thereby bonding and fixing the elastic film 2015 and the second sealing layer 2014 to each other.
  • the adhesive layer 200C also has an opening 2001.
  • the opening 2001 is also located in the area where the elastic film 2015 and the second sealing layer 2014 overlap each other. For example, the opening 2001 is completely located in the elastic film.
  • the film 2015 and the second sealing layer 2014 are in an area where the film 2015 and the second sealing layer 2014 are facing and overlapping each other, and the area where the elastic film 2015 and the second sealing layer 2014 are facing and overlapping each other is completely bonded and fixed by the adhesive layer 200C.
  • the acting channel 2016 allows when an external force acts on the elastic membrane 2015 to deform the elastic membrane 2015, the deformed elastic membrane 2015 partially passes through the opening 2001 of the adhesive layer 200C, thereby enabling the elastic membrane 2015 to be punctured without being punctured.
  • the external force also acts on the second sealing layer 2014 to puncture the second sealing layer 2014. After the external force is removed, the elastic film 2015 will basically return to its original shape.
  • the material of the elastic film 2015 may be a composite polymer material, such as a composite material of polystyrene (PS) and polyethylene terephthalate (PET). Therefore, the elastic film 2015 can have good elasticity and strength at the same time.
  • PS polystyrene
  • PET polyethylene terephthalate
  • the sampling structure mounting portion 2011 and the reagent cell 2012 are provided on the upper substrate 200A, and the adhesive layer 200C is attached to the surface of the upper substrate 200A (shown as the lower surface in the figure) and the elastic film 2015 is fixed on the upper substrate 200A. on the surface.
  • the lower substrate 200B includes a substrate opening to provide the action channel 2016, for example, the elastic film 2015 completely covers the action channel 2016.
  • the adhesive layer 200C at least partially exposes the second sealing layer 2014 through the opening 2001, so that the deformed elastic film 2015 can act on the second sealing layer 2014 to pierce the second sealing layer 2014.
  • the projection of the third opening 1001 of the adhesive layer 200C on the lower substrate 100B at least partially overlaps with the acting channel 1016, for example, is completely located in the acting channel 1016.
  • the detection chip 200 may further include a sample mixing chamber 206 disposed between the sample injection structure 201 and the sample filtering structure 203.
  • the sample mixing chamber 206 can be used to mix the tested sample with the diluent in the reagent tank.
  • the detection system provided in this embodiment includes a sampling structure 210 and a packaging structure 211 in addition to the detection chip 200 described above.
  • the sampling structure 210 is installed in the sampling structure installation part 2011, and the packaging structure 211 is used to seal the sampling structure 210.
  • the packaging structure 211 is a silicone cap, which has better elasticity and sealing properties, and the sample can be mixed through the cooperation of the sampling structure 210 and the packaging structure 211.
  • the packaging structure 211 includes a sealing portion 211A and a fixing portion 211B.
  • the sealing portion 211A is used to seal the sampling structure 210
  • the fixing portion 211B is used to fix the sampling structure 210 to the detection chip 200.
  • the sealing portion 211A and the fixing portion 211B are an integrally formed silicone structure.
  • the fixing structure 211B is an annular socket structure.
  • the sealing portion 211A may also have structures such as a main body portion and a vent hole.
  • structures such as a main body portion and a vent hole.
  • the detection system further includes a movable first jack 220, and the first jack 220 is disposed on a side of the packaging structure 211 away from the sampling structure 210.
  • the first jack 220 can apply a force to the sealing portion 211A of the packaging structure 211.
  • the detection system may further include a movable second ejector pin 230, and the second ejector pin 230 is used to puncture the second sealing layer 2014.
  • the second jack 230 may apply a force to the second sealing layer 2014 through the acting channel 2016 and the elastic mold 2015 in the lower substrate 100B.
  • the sampling structure 210 is used to suck the sample to be tested.
  • the sampling structure 210 may be any structure having a sampling function.
  • the sample to be detected may be, for example, blood, body fluid, etc., which is not limited in the embodiments of the present disclosure.
  • the sampling structure 210 is mounted on the detection chip 200, and the packaging structure 211 is used to fix and seal the sampling structure 210.
  • the bottom of the sampling structure 210 extends into the reagent pool 2012 of the detection chip 200.
  • the upper surface of the reagent pool 2012 has a first sealing layer 2013 for sealing.
  • the second ejector rod 230 is driven to move upward, so that the second ejector rod 230 applies a force to the second sealing layer 2014 under the reagent tank 2012 through the acting channel 2016 and the elastic membrane 2015, so as to puncture the second sealing layer 2014. Since the elastic membrane 2015 is elastic, it can be restored to its original state after the external force is removed. After the second sealing layer 2014 is punctured, the reagent cell 1012 is in communication with the sample mixing chamber 206.
  • the first ejector rod 220 is used to apply a force to the sealing portion 211A of the sealing structure 211 at the first speed. At this time, the pressure inside the sampling structure 210 increases, so that the tested sample is pushed out of the sampling structure 210 and enters the reagent pool In 2012, further, the tested sample and the diluent in the reagent pool 2012 can be mixed and can enter the sample mixing chamber 206.
  • the first ejector rod 220 is withdrawn at the second speed.
  • the sealing portion 211A rebounds, and the mixed solution of the diluent and the tested sample will be sucked back into the reagent tank 2012.
  • the mixed solution of the diluent and the tested sample can reciprocate in the reagent pool 2012 and the mixing chamber 206, thereby completing the mixing operation.
  • the first speed at which the first jack 220 applies force to the sealing portion 211A is lower than the second speed at which the first jack 220 withdraws, that is, the first jack 220 is slowly pressed down and quickly raised. This operation has Helps improve the mixing effect of the tested sample and the diluent.
  • the above mixing operation can be performed multiple times, so that the mixed solution reciprocates multiple times in the reagent tank 2012 and the mixing chamber 206 to further improve the mixing effect of the tested sample and the diluent.
  • FIG. 14 shows the flow path of the sample to be detected in the detection chip 200 of the above-mentioned embodiment.
  • the slow and continuous pressing operation of the first ejector rod 220 can be continued, so that the tested sample enters the sample filter structure 203 (for example, the tested sample is input from the side direction and the direction perpendicular to the plane of the filter membrane 2031 at the same time).
  • the filtered sample to be tested can be transported to the sample detection structure 202 during the continuous pressing of the first ejector 220 (the sample to be tested is output to the sample detection structure 202 from the side, for example), Finally, it is transported into the detection cavity 2021 of the sample detection structure 202.
  • different detection cavities 2021 have freeze-dried reagents suitable for different detection items, so that after the sample to be tested can react with the freeze-dried reagent, the sample detection structure 202 starts to perform detection and outputs the detection result.
  • the first ejector rod 220 is always kept in a depressed state, so as to avoid the backflow of the tested sample.
  • the detection system can realize an automated detection process, and the detection system can also obtain more accurate detection results.
  • the detection system can also be used in conjunction with a sampling structure with acquisition and mixing functions, for example, in conjunction with the sampling structure shown in FIGS. 7 and 9-10, for example, it can also be used in some examples.
  • a sampling structure with acquisition and mixing functions for example, in conjunction with the sampling structure shown in FIGS. 7 and 9-10, for example, it can also be used in some examples.
  • the detection system can also perform a detection operation through an operation process similar to that provided in the foregoing embodiment.
  • the embodiments of the present disclosure do not specifically limit the operation process of the detection system.
  • FIG. 15 shows an exploded top view of the detection system provided by this embodiment.
  • the detection chip of the detection system of this embodiment is a modification of the detection chip and detection system shown in FIGS. 12 and 13, and the difference from the detection chip and detection system shown in FIGS. 12 and 13 is that the detection chip only includes the upper substrate 300A.
  • the lower substrate is not included.
  • the elastic membrane 3015 located on the second side (the lower side in the figure) of the reagent cell covers the lower surface of the upper substrate 300A in the figure, instead of covering only the reagent cell and its surrounding area, thus the elastic membrane 3015 also functions as the lower substrate in the detection chip shown in FIG. 12 and FIG. 13.
  • the parts of the embodiment shown in FIG. 15 that are the same as the embodiments shown in FIG. 12 and FIG. 13 will not be repeated here.
  • the material of the elastic film 3015 may be a composite polymer material, such as a composite material of polystyrene (PS) and polyethylene terephthalate (PET). Therefore, the elastic film 2015 can have good elasticity and strength at the same time.
  • PS polystyrene
  • PET polyethylene terephthalate
  • the adhesive layer 300C is attached to the lower surface of the upper substrate 300A in the figure and fixes the elastic film 3015 on the lower surface of the upper substrate 300A.
  • the adhesive layer 300C also has an opening 3001.
  • the ejector rod can directly act on the elastic mold 3015, and exert a force on the sealing layer on one side of the reagent tank through the opening 3001 of the adhesive layer 300C.

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Abstract

L'invention concerne une puce d'essai et un système d'essai. La puce d'essai comprend une structure d'injection d'échantillon (101), une structure de test d'échantillon (102) et une structure de filtration d'échantillon (103). La structure d'injection d'échantillon (101) est utilisé pour injecter un échantillon d'essai, la structure d'essai d'échantillon (102) est utilisé pour permettre à l'échantillon d'essai d'être analysé, et la structure de filtration d'échantillon (103) est disposée entre la structure d'injection d'échantillon (101) et la structure d'essai d'échantillon (102) et est respectivement en communication avec la structure d'injection d'échantillon (101) et la structure d'essai d'échantillon (102), de manière à filtrer l'échantillon d'essai injecté au moyen d'une chromatographie latérale et à transmettre l'échantillon d'essai filtré à la structure d'essai d'échantillon (102). La puce d'essai peut amener le dispositif d'essai d'échantillon d'essai au moyen de la structure de filtration d'échantillon (103), rendant ainsi un résultat d'essai plus précis, et facilitant l'amincissement de la puce d'essai.
PCT/CN2021/093087 2019-06-26 2021-05-11 Puce d'essai et système d'essai Ceased WO2021244238A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201910562599 2019-06-26
CN202010495808.7 2020-06-03
CN202010495808.7A CN113101985B (zh) 2019-06-26 2020-06-03 检测芯片和检测系统

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WO2021244238A1 true WO2021244238A1 (fr) 2021-12-09

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Cited By (1)

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