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WO2021243333A3 - Test d'acide nucléique de masse - Google Patents

Test d'acide nucléique de masse Download PDF

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Publication number
WO2021243333A3
WO2021243333A3 PCT/US2021/035240 US2021035240W WO2021243333A3 WO 2021243333 A3 WO2021243333 A3 WO 2021243333A3 US 2021035240 W US2021035240 W US 2021035240W WO 2021243333 A3 WO2021243333 A3 WO 2021243333A3
Authority
WO
WIPO (PCT)
Prior art keywords
rna
sample
sequencing
pathogen
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2021/035240
Other languages
English (en)
Other versions
WO2021243333A2 (fr
Inventor
David Troy MOORE
Katherine Elena VARLEY
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biohsv Holdings Inc
Original Assignee
Biohsv Holdings Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biohsv Holdings Inc filed Critical Biohsv Holdings Inc
Priority to EP21813023.5A priority Critical patent/EP4157300A4/fr
Publication of WO2021243333A2 publication Critical patent/WO2021243333A2/fr
Publication of WO2021243333A3 publication Critical patent/WO2021243333A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de criblage de grandes populations de sujets en vue de déterminer la présence d'un ARN, tel que l'ARN provenant d'un pathogène. Le procédé combine le regroupement d'échantillons, la RT-PCR ciblée, l'utilisation d'amorces de PCR universelles et le séquençage parallèle massif. Le procédé est extrêmement sensible, et s'il est combiné à un séquençage du génome entier, confère une spécificité extrêmement élevée combinée à une résilience élevée à une variation génétique potentielle chez un pathogène. Le procédé peut être utilisé pour insérer un "code barres" unique qui identifie l'échantillon spécifique (tel qu'un échantillon provenant d'un patient individuel), dans l'ADN produit à partir de l'ARN, ce qui permet à l'ADN provenant de nombreux échantillons d'être regroupé en vue d'un séquençage tout en permettant encore l'identification d'éventuels échantillons individuels dont le test est positif.
PCT/US2021/035240 2020-05-29 2021-06-01 Test d'acide nucléique de masse Ceased WO2021243333A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP21813023.5A EP4157300A4 (fr) 2020-05-29 2021-06-01 Test d'acide nucléique de masse

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US202063032120P 2020-05-29 2020-05-29
US63/032,120 2020-05-29
US202063125501P 2020-12-15 2020-12-15
US63/125,501 2020-12-15

Publications (2)

Publication Number Publication Date
WO2021243333A2 WO2021243333A2 (fr) 2021-12-02
WO2021243333A3 true WO2021243333A3 (fr) 2022-03-03

Family

ID=78722930

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2021/035240 Ceased WO2021243333A2 (fr) 2020-05-29 2021-06-01 Test d'acide nucléique de masse

Country Status (2)

Country Link
EP (1) EP4157300A4 (fr)
WO (1) WO2021243333A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022029157A2 (fr) * 2020-08-06 2022-02-10 F. Hoffmann-La Roche Ag Compositions et procédés pour détecter le coronavirus 2 du syndrome respiratoire aigu sévère (sars-2), de la grippe a et de la grippe b

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004097040A1 (fr) * 2003-04-30 2004-11-11 4Base Lab Gmbh Advanced Molecular Analysis Procede de detection de virus d'un brin(+) d'arn infectieux, en particulier, d'enterovirus infectieux
US20120010091A1 (en) * 2009-03-30 2012-01-12 Illumina, Inc. Gene expression analysis in single cells
US20180148757A1 (en) * 2008-09-05 2018-05-31 Washington University Method for multiplexed nucleic acid patch polymerase chain reaction
US20180237835A1 (en) * 2015-07-31 2018-08-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Serv Methods of analyzing virus-derived therapeutics

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004097040A1 (fr) * 2003-04-30 2004-11-11 4Base Lab Gmbh Advanced Molecular Analysis Procede de detection de virus d'un brin(+) d'arn infectieux, en particulier, d'enterovirus infectieux
US20180148757A1 (en) * 2008-09-05 2018-05-31 Washington University Method for multiplexed nucleic acid patch polymerase chain reaction
US20120010091A1 (en) * 2009-03-30 2012-01-12 Illumina, Inc. Gene expression analysis in single cells
US20180237835A1 (en) * 2015-07-31 2018-08-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Serv Methods of analyzing virus-derived therapeutics

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE NUCLEOTIDE 14 April 2020 (2020-04-14), ANONYMOUS : "Cloning vector pSF_lenti_SARS-CoV-2_partial-ORF1a, complete sequence", XP055910286, retrieved from GENBANK Database accession no. MT299802 *
GONZALEZ-REICHE ANA S., HERNANDEZ MATTHEW M., SULLIVAN MITCHELL, CIFERRI BRIANNE, ALSHAMMARY HALA, OBLA AJAY, FABRE SHELCIE, KLEIN: "Introductions and early spread of SARS-CoV-2 in the New York City area", MEDRXIV, 16 April 2020 (2020-04-16), pages 1 - 22, XP055910291, [retrieved on 20220407], DOI: 10.1101/2020.04.08.20056929 *

Also Published As

Publication number Publication date
EP4157300A2 (fr) 2023-04-05
EP4157300A4 (fr) 2024-07-17
WO2021243333A2 (fr) 2021-12-02

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