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WO2021191929A1 - Huile riche en bêta-carotène - Google Patents

Huile riche en bêta-carotène Download PDF

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Publication number
WO2021191929A1
WO2021191929A1 PCT/IN2021/050304 IN2021050304W WO2021191929A1 WO 2021191929 A1 WO2021191929 A1 WO 2021191929A1 IN 2021050304 W IN2021050304 W IN 2021050304W WO 2021191929 A1 WO2021191929 A1 WO 2021191929A1
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WIPO (PCT)
Prior art keywords
beta
carotene
oil
composition
rich
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Ceased
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PCT/IN2021/050304
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English (en)
Inventor
Chitra Vasant Savangikar
Vasant Anantrao Savangikar
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Individual
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Priority to CN202180038421.1A priority Critical patent/CN115734714A/zh
Publication of WO2021191929A1 publication Critical patent/WO2021191929A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings or cooking oils
    • A23D9/007—Other edible oils or fats, e.g. shortenings or cooking oils characterised by ingredients other than fatty acid triglycerides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/12—Production of fats or fatty oils from raw materials by melting out
    • C11B1/14—Production of fats or fatty oils from raw materials by melting out with hot water or aqueous solutions
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B13/00—Recovery of fats, fatty oils or fatty acids from waste materials
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/74—Recovery of fats, fatty oils, fatty acids or other fatty substances, e.g. lanolin or waxes

Definitions

  • This invention relates to oil extracts from isolated beta-Carotene crystals, beta-Carotene rich fruits, underground parts of plants, flowers and green leafy biomass, method of making the oil extracts and to standardize them for beta-Carotene content for health benefit.
  • Covid-19 has no medicine nor vaccine.
  • the Innate Immunity also known as Non specific Immunity is a resource that is mediated through Natural Killer Cells that have capability to protect a person from not only Coronavirus from but any microbial pathogen that enters body as well as cancer cells. Vaccines are specific to the strain of microorganism against which they are prepared. Non-specific Immunity, as per its name, is not specific to any pathogen.
  • Natural Killer Cells which mediate the non-specific immunity, detect any cell of the body as “non-self” when it is infected with any and every pathogen that enters in body, or it is an early stage cancer cell and is yet to multiply and establish a nodule of cancer. Natural Killer Cell attaches itself to that “non-self’, injects its lethal charge into the “non-self’ cell to not only kill it but also to destroy the nucleic acid of the cell itself as well as that of the pathogen contained in the “non-self” cell to make sure that the infection is eradicated completely.
  • beta-Carotene has very low bioavailability, consumption of doses of beta- Carotene to the tune of 50mg alternate day used by Santos et al 1 . are too high as compared to epidemiological consumption of beta-Carotene, which in North America is 1.5 mg/day as mentioned by Hughes (1999) 2 and also from the view of practical impossibility of availability of beta-Carotene to that extent for daily consumption from ordinarily available food sources; and further, whether that high a consumption of beta-Carotene would be safe in the long run is also not known.
  • beta-Carotene Purified preparations of beta-Carotene are available in soft-gel form which usually carry 25000 IU of beta-Carotene, for which consumption of one capsule per day is recommended on the label.
  • beta-Carotene has only 0.08% solubility in oil at room temperature. Consequently, purified Beta-Carotene capsules in oil suspension shall have a very low bioavailability. This is evident from very high doses of the beta-Carotene used in clinical trials on improvement of Natural Killer Cells in adults to a tune of 50mg per alternate day for 38 middle- aged men, 51-64 y; 21 elderly men, 65-86 y as disclosed by Santos et al. (1996) 1 .
  • the dose used was also much smaller than 50 mg/day used for crystalline beta-Carotene in earlier reported prospective trials and no adverse effect was reported on smokers.
  • use of natural source of beta-Carotene rather than synthetic source and at a smaller dose than 30mg per day was seen to be safer and equally effective at about 2.5 times smaller dose.
  • NK Cell activity increased significantly over the baseline and washout levels of in PSPL in the first week itself, whereas carrot diet required two weeks to improve the NK Cell Activity and that was at a level at which the PSPL resulted in 1 week. It was concluded that consumption of PSPL modulates various immune functions including increased proliferation responsiveness of PBMC, secretion of cytokines IL-2 and IL-4, and the lytic activity of NK cells. Increase in lytic activity of Natural Killer Cells was about 3 times over baseline within two weeks.
  • PSPL or any other equivalent green leafy vegetable
  • composition of oil with beta-Carotene dissolved in it in a quantity standardized for a quantity per dosage form that is effective for a health benefit available from beta-Carotene comprises, without limitation, beta- Carotene rich oil produced by a plant, extract of a plant part rich in beta-Carotene extracted in oil suitable for human consumption suitable for human consumption and the like.
  • the beta- Carotene rich oil produced by a plant comprises, without limitation, Red Palm Oil/Palm Kernel oil and the like;
  • extract of a beta-Carotene rich plant part comprises, without limitation, beta- Carotene rich fruits, underground parts, green leafy biomass and flowers of plants.
  • the source of composition of oil according to this invention also includes crystallized/solidified compositions of purified beta-Carotene.
  • the beta-Carotene rich fruits comprise, without limitation, Tomato, (, Lycopersicon esculentum L.) Papaya ( Carica papaya L.) and Mango ( Mangifera indica L. ).
  • the beta-Carotene rich underground parts of plants comprise, without limitations, carrot ( Daucus carota L.) and Sweet Potato ( Ipomoea batatas L) variety rich in beta-Carotene.
  • the green leafy biomass comprise, without limitation Green leafy vegetable, Green leaves of cole crops, trees, shrubs.
  • the beta-Carotene rich flowers comprise one or more, without limitations, Marigold (Tagetus erecta L.), Dandelion ( Taraxacum officinale L.), Saundersonia aurantiaca HOOK., Cameiiia crysantha HU., Ipomoea sp., Chrysanthemum (Chrysanthemum morifoliumindicum RAMAT.) and the like.
  • the health benefit available from beta-Carotene comprises, without limitation, improvement in Natural Killer Cell Activity, anti-allergy property, improving glow and health of skin, as a pro vitamin A for maintaining optimum level of Vitamin A within the body and the like.
  • the health benefit from improvement in Natural Killer Cell Activity comprises, without limitation, First Line Innate Immunity from multiplication of pathogenic micro-organisms and pre-cancerous cells within the body.
  • Dosage form of the composition of the oil according to this invention comprises, without limitation, liquid oil, soft gelatine capsules, oil filled hard gelatine capsules, emulsions of various types, sachets of micro-capsules and gummies.
  • daily dose through the dosage forms comprises at least 418pg beta-Carotene with appropriate overages.
  • the per day dosage of the composition of the oil according to this invention comprises, without limitation, 5ml of liquid oil, or 2 to 3 soft-gelatin capsules.
  • This invention also discloses a process of making composition of oil with beta-Carotene dissolved in it in a quantity standardized for a quantity per dosage form that is effective for a health benefit available from beta-Carotene.
  • the process comprising steps of determining beta- Carotene content of beta-Carotene rich oil produced by a plant, or of an extract of a beta- Carotene rich plant part in oil suitable for human consumption, or of a solution of oil suspension of beta-Carotene crystals. If the content is greater than the target content of standard chosen for beta-Carotene, diluting the same by adding edible oil to adjust the content to the desired value is resorted to.
  • a part removal of the oil may be achieved by use of a detergent, soap or alkali to convert a part of the oil to water soluble component, and removing the water soluble component by adding water and removing the aqueous layer below the oil layer to leave behind more concentrated oil with respect to standardized content of beta-Carotene.
  • a beta-Carotene rich plant part in oil suitable for human consumption is made by steps comprising blanching a beta-Carotene rich plant part if required, comminuting the plant part, if required, removing as much moisture/water as possible by application of heat, contacting the comminuted plant part with an extractant comprising an edible oil or in combination a solvent for beta-Carotene and edible oil, allowing maximum possible dissolution of beta-Carotene in the extractant, removing the solvent for beta-Carotene, and recovering the oil as an extractthe solvent for beta-Carotene comprises a non-oil solvent permitted for solvent extraction of foods.
  • the non-oil solvent comprises, without limitation, a group comprising hexane and supercritical Carbon Dioxide.
  • compositions comprising a composition of oil with beta-Carotene dissolved in it in a quantity standardized for a quantity per dosage form that is effective for a health benefit available from beta-Carotene as an ingredient, the composition of oil with beta-Carotene.
  • Such compositions include, a composition for topical application to skin, a hair oil fortified with a composition of oil with beta-Carotene dissolved in it as an ingredient, and a cream or a lotion to be applied topically on the skin.
  • the composition for topical application to skin being for anti-allergy, for skin nourishment or for cosmetic purpose.
  • This invention also includes an extract of a plant part rich in beta-Carotene extracted in oil suitable for human consumption, the composition being made from a plant part other than green leafy biomass.
  • beta-Carotene that was consumed during the clinical trial; and has been chosen at present as the standard at which the Innate Immunity Booster should be standardized.
  • the bioavailability of beta-Carotene in terms of weight of Vitamin A converted in the body is 28: 1 (Tang 2010 8 ). Since one molecule of Vitamin A is equivalent to half the molecule of beta-Carotene, the bioavailability in terms of weight of beta- Carotene molecules fed through green leafy vegetables to biologically available beta-Carotene is 56:1.
  • beta-Carotene in its natural plant derived raw materials are also known to be very low in bio-availability due to very low release in the digestive tract from the plant matrix in which beta-Carotene is embedded. Solubility in edible oils is also very low: 0.08% at room temperature, 0.2% at 60 °C and 0.8% at 100 °C. Hence, where beta-Carotene is the active ingredient of targeted health effect, its bioavailability in its carrier raw or processed food material is of paramount importance rather than its chemical content.
  • Chylomicrons are found in the blood and lymphatic fluid where they serve to transport fat from its port of entry in the intestine to the liver and to adipose (fat) tissue.” Thus, to ensure measured reasonable dose of beta-Carotene to get practically 100% absorbed, we decided to get edible oil solution of beta- Carotene. It may be noted here that although knowledge of Chylomicron exists for a long time, the problem of providing measured accurate easily manageable dosing of highly bioavailable beta-Carotene did exist since a long time, including Chen et al (2005), we are the first to devise oil solutions of beta-Carotene for this purpose.
  • this invention discloses an oil comprising Beta-Carotene that is extracted.
  • this invention discloses an oil extract made from a food source is standardized for quantity of beta-Carotene per dose effective for health benefit.
  • This invention also embodies process/es meant for making oils and oil extracts from beta- Carotene standardized for quantity of beta-Carotene per dose effective for health benefit rich food sources.
  • the oil extracts of this invention are made from beta-Carotene rich plant parts.
  • the beta-Carotene rich plant parts include, without limitations, fruits, underground parts, green leafy biomass and flowers.
  • oil extracts can be prepared to extract beta-Carotene dissolved in edible oil, define what is an effective level of the same for health benefit and standardize the oil extract to the required concentration in volume suitable for daily consumption.
  • the health benefit that can be targeted would be, without limitation, for modulating regulating improving Natural Killer Cell Activity, anti-allergy activity, cosmetic applications.
  • the Natural Killer Cell Activity includes immunity from infectious diseases and keeping body free from cancer cells.
  • this invention discloses an oil solution comprising beta-Carotene. In another aspect, this invention discloses an oil solution standardized for an amount of beta-Carotene effective in a daily dose for improvement in Natural Killer Cells Activity. In a further another aspect, this invention discloses an oil solution standardized for an amount of beta-Carotene effective in a daily dose for improvement in Natural Killer Cells Activity for reduction in the risk of infection from pathogenic microorganisms and the risk of cancer.
  • composition for topical application comprising the oil of this invention comprising effective level of Beta-Carotene would be useful for skin health for cosmetic purposes and also as First Line response to skin diseases arising from pathogenic microorganisms, including, without limitation, herpis zoster, eczema or rash and hives arising from allergies.
  • This invention also includes the beta-Carotene rich fruit oil when it is standardized to contain an amount of beta-Carotene effective for improvement in Natural Killer Cells Activity.
  • This invention further also includes the beta-Carotene rich fruit oil includes Crude Palm Oil, also known as Red Palm Oil when it is standardized to contain an amount of beta-Carotene effective for improvement in Natural Killer Cells Activity for reduction in the risk of infection from pathogenic microorganisms and the risk of cancer.
  • the beta-Carotene rich fruit oil includes, without limitation. Crude Palm Oil, also known as Red Palm Oil also known as Palm Kernel Oil.
  • Oil extracts of beta-Carotene rich fruits, underground storage organs and flowers can be made in several different ways. Some of which are illustrated below, which are non-limiting examples; and these examples do not restrict the scope of the claims of this invention.
  • Crude Palm oil also known as Red Palm Oil extracted from mesocarp of the fruit of palm tree (Elaeis guineensis) is rich in beta-Carotene to a tune of around more than 250pg/g oil. It is an aspect of this invention that Crude Palm oil /Red Palm oil, when standardized for a dosage per day that has beta-Carotene content in daily dose effective for improvement in Natural Killer Cell Activity is useful for reducing the risk of infection from pathogenic microorganisms. This also includes reduction in the risk of infection from the novel Coronavirus.
  • Crude Palm Oil / Red Palm Oil for reduction in the risk of infection from pathogenic microorganisms; in general, and from the novel Coronavirus infection in particular has inventive step because this has not been contemplated by any person ordinarily skilled in the art of Clinical Nutrition or healthcare before it is proposed here. It is surprising that Crude Palm Oil or Red Palm oil has never been proposed/contemplated for use for reduction in the risk of infection from pathogenic microorganisms in general.
  • Crude Palm Oil / Red Palm Oil may be used as an oil standardized for effective dosage volume or as encapsulated for reduction in the risk of infection from pathogenic microorganisms.
  • beta-Carotene in oil shall be in bio-available form and the same provided from any source of beta-Carotene at an effective daily dose for improving activity of Natural Killer Cells would be an Innate Immunity Booster/Natural Killer Cells Activity Booster useful as first line response for any viral/bacterial communicable disease, including Novel Coronavirus.
  • the composition of oil containing Beta-Carotene would enable maintaining safely Vitamin A level at optimum level throughout the pregnancy period, which is vitally necessary to ensure that all systems of the fetus develop to its full genetic potential.
  • Managing to maintain optimum level of Vitamin A throughout pregnancy period is not possible with synthetic Vitamin A since it become readily toxic if it raises Vitamin A level within the body even a little above optimum level within the body, and to avoid such toxicity, if the synthetic Vitamin A is provided at defensive level, the level of Vitamin A in body readily drops down.
  • substantially dilute but efficacious and safer oil solutions containing 460 pg beta-Carotene per 5 ml are made as Innate Immunity Booster/ NK Cell Activity Booster. This constitutes substantial technical advance against the prior art use of beta-Carotene.
  • Rice Bran oil is used.
  • any other edible oil can be used such as Groundnut oil, Safflower oil, Soybean oil, Coconut oil, Mustard oil, Fish oil, Algal oil, Castor oil, Sesame oil, Flaxseed oil, Palm oil, Olive Oil and any other edible oil.
  • Borel et al (1998 11 ) have shown that Chylomicron beta-Carotene and retinyl palmitate responses are dramatically diminished when men ingest beta-Carotene with medium-chain rather than long-chain triglycerides.
  • edible oils rich in long chain fatty acids would be preferable; which include, without limitation, Soybean oil and Olive oil.
  • this invention comprises oil extract of leaf nutrient concentrate, wherein the quantity of beta-Carotene dissolved in it is standardized per dosage form that is effective for a health benefit available from beta-Carotene,
  • the oil extract according to above described invention has anti-allergy properties. Examples 7 and 8 illustrate this use.
  • the oil extract of tomato of this invention would also have potential use for treating allergies; which is a novel property of the oil extract of tomato. This property is especially useful in the context of vaccination drive for COVID-19, since all vaccines have risk of allergies for those who are prone for allergies. This is particularly an aggravated risk for vaccines based on messenger RNA technology. This oil extract of tomato would potentially be an answer for this problem.
  • Extracting beta-Carotene from plant parts is conventionally done using organic solvents such as hexane, acetone and methanol and their mixtures.
  • organic solvents such as hexane, acetone and methanol and their mixtures.
  • the isolated solvent extract with all solvent soluble components or crystallized beta-Carotene can thereafter be dissolved in oil after solvent removal to make oil solution of beta-Carotene.
  • This method has also been illustrated and is one aspect of this invention. However, that is considered as a method of low in priority, since the residual raw material left after solvent extraction as well as the beta-Carotene-containing fractions retain solvent residues which are undesirable feature and solvent recovery of entire process becomes costly.
  • Weight of content of a 15mg beta-Carotene capsule in one such capsule sourced form market, excluding weight of the capsule shell is 160mg. No information is given on how much is Soybean oil in the capsule. Presuming that the same is 100mg, in view of solubility of 0.08mg beta-Carotene in 100 mg oil, the capsule contains 80 pg Beta-Carotene dissolved in oil (bio- available); and rest 14.92 mg beta-Carotene in a form that is not bioavailable.
  • beta-Carotene After consuming with food, further dissolution of the un-dissolved beta-Carotene derived from the soft-gelatin capsule will depend upon how much oil is contained in the food consumed concurrently with the capsule and only that much beta-Carotene will get dissolved in the available oils in the food.
  • solubility in oil at room temperature for each 5ml oil provided no other oil soluble material is dissolved in it, 4000 pg beta-Carotene will theoretically get absorbed from the 15000 pg beta-Carotene; however, in practice far less beta-Carotene will get dissolved due to competitive inhibition of dissolution if the oil has already dissolved other fat soluble components from the food.
  • beta-Carotene there is an uncertainty of how much beta-Carotene will be bio-available from such a capsule for one individual, which shall differ from another individual due to differences in case-to-case for above variables. Thus, it is certain that a major portion of the beta-Carotene contained in a 15mg capsule will invariably be wasted. Further, even if, in some cases, it gets dissolved more than envisaged above, that may be injurious to smokers and it is not known how much is safe for a chronic overdose for non-smokers too.
  • a 15mg beta-Carotene capsule shall give 32.6 doses if its content dissolved in oil, which would give same effect as the consumption of one soft-gel containing 15mg beta-Carotene.
  • 32.6 doses of 460 pg beta-Carotene each in oil form or in soft-gel form shall be a rational product for improving activity of compromised Natural Killer Cells.
  • oil extract without encapsulation may be the most rational/preferred way of consumption than its soft-gel because it is possible to ensure that the oil extract is well mixed with some component of food, which can be eaten with rest of the food of a major meal to ensure that all of the oil extract gets to the small particulate level which gets converted and absorbed in blood stream from the digestive tract in the form of Chylomicron which ensures complete bioavailability of the beta-beta-Carotene content of the dose.
  • a chylomicron is “a lipoprotein rich in triglyceride and common in the blood during fat digestion and assimilation” as per Merriam-Webster dictionary on the link https://www.merriam- webster.com/dictionary/chylomicron 12 .
  • beta-Carotene content in the samples were analysed by Ultraviolet-Visible (UV-vis) spectrophotometer at 446 nm using MPOB test method.
  • the sample was homogenized and weighed to the nearest ⁇ 0.0001 g into a 25 ml. volumetric flask.
  • the sample was dissolved with n-hexane and diluted to the mark.
  • the solution was transferred into a 1 cm quartz cuvette and the absorbance was measured at 446 nm against n-hexane.
  • the beta-Carotene content of different vegetable oils is defined and calculated as beta-Carotene in parts per million (ppm).
  • Carotenoids content [V x 383 x (As-Ab)]/ (100*W)
  • Spectrophotometric analyses done at 446 nm were outsourced and colorimetric analyses at 460nm were done in-house.
  • Oil extracts were made by using same method from the pomace and precipitate obtained from Papaya fruit and carrots.
  • Crude Palm oil also known as Red Palm Oil extracted from mesocarp of the fruit of palm tree (Elaeis guineensis) is rich in beta-Carotene to a tune of around more than 250pg/g oil.
  • beta-Carotene content is adjusted accurately to what is required for making softgels from the Red Palm Oil, if there is a requirement of concentrating the content to some extent, the above technique of concentration the Red Palm Oil or any other beta-Carotene containing oil is now available.
  • Oil Extract contained 1336pg beta- Carotene/5ml.
  • soft-gel capsules were 5 year old. By taking recently made soft-gel containing 15mg shall provide 32 portions of oil solution containing 460pg beta-Carotene/5ml.
  • oil soluble beta beta-Carotene wherein 1 ml of the oil extract shall have at least 460 pg beta- Carotene; so that two soft-gels would give daily dose of the Innate Immunity Booster/NK Cell Activity Booster made from the method of this invention.
  • Soft-gels of oil solution would be convenient for those for whom oil extract is not palatable.
  • oil extract of tomato containing 418pg beta-Carotene/5 ml has stopped the person getting allergy from dust.
  • the 69 year old person had dust allergy since 1998.
  • He started using this oil extract product named as Lelmmune-T, since last week of August 2020 mainly as a Natural Killer Cell Activity booster in view of the threat of COVID-19 pandemic.
  • Hexane 20ml was added to 25g Tomato paste (dry weight 12.21 g) and mixed well.
  • Six gram Rice Bran oil was added to the mix and mixed further.
  • the hexane with dissolved beta-Carotene and oil was poured out; hexane was allowed to evaporate. This yielded 6.75 g oil extract comprising beta-Carotene.
  • the oil extract was determined to comprise 688pg/5ml beta- Carotene. This can be diluted to 418pg/5ml beta-Carotene plus desired overages, which would be 10% or 30% needing on the standard for overages chosen.
  • other solvents in which beta-Carotene is soluble including supercritical extraction by supercritical fluids in which beta-Carotene is soluble can be done.
  • Such supercritical fluids include, without limitation, Supercritical C0 2 .
  • Methi Feugreek; Trigonell foenum-graecum L
  • Methi Trigonell foenum-graecum L
  • the juice was added slowly to boiling water in a container to keep the water ina pan in boiling state.
  • the juice coagulated to provide a green precipitate, the Leaf Nutrient Concentrate, which was removed by filtration through a cotton cloth and dried.
  • the yield was about 60 gram after air drying.

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Abstract

La présente invention divulgue une composition d'huile comportant du beta-carotène dissous dans ladite composition en une certaine quantité avec ou sans normalisation, afin d'obtenir une quantité par forme galénique efficace pour un bénéfice pour la santé proposé par le bêta-carotène ; et un procédé de préparation associé. Elle comprend une huile produite par une plante normalisée, un extrait d'une partie de plante dans de l'huile appropriée pour la consommation humaine, et de l'huile dans laquelle du beta-carotène purifié cristallisé/solidifié est dissous. Le bénéfice pour la santé disponible comprend une amélioration de l'activité des cellules tueuses naturelles assurant une immunité innée contre la multiplication de microorganismes pathogènes et de cellules précancéreuses dans le corps, des propriétés antihistaminiques, une amélioration de l'éclat et de la santé de la peau, sous forme de provitamine A permettant de maintenir un niveau optimal de vitamine A dans le corps pendant toute la période de grossesse. L'invention concerne également des compositions utilisant la composition d'huile de l'invention en tant qu'ingrédient.
PCT/IN2021/050304 2020-03-24 2021-03-23 Huile riche en bêta-carotène Ceased WO2021191929A1 (fr)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024116212A1 (fr) * 2022-12-03 2024-06-06 Chitra Vasant Savangikar Extrait de triglycérides riche en bêta-carotène : amélioration du procédé de production et utilisation de sous-produits

Family Cites Families (5)

* Cited by examiner, † Cited by third party
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