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WO2021172811A1 - Incubateur et système de culture pour fournir un environnement similaire au corps humain, comprenant une unité de culture cellulaire - Google Patents

Incubateur et système de culture pour fournir un environnement similaire au corps humain, comprenant une unité de culture cellulaire Download PDF

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Publication number
WO2021172811A1
WO2021172811A1 PCT/KR2021/002072 KR2021002072W WO2021172811A1 WO 2021172811 A1 WO2021172811 A1 WO 2021172811A1 KR 2021002072 W KR2021002072 W KR 2021002072W WO 2021172811 A1 WO2021172811 A1 WO 2021172811A1
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WO
WIPO (PCT)
Prior art keywords
unit
culture
elastic
cell culture
tension
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2021/002072
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English (en)
Korean (ko)
Inventor
김백길
조남훈
장연수
강숙희
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Industry Academic Cooperation Foundation of Yonsei University
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Industry Academic Cooperation Foundation of Yonsei University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Industry Academic Cooperation Foundation of Yonsei University filed Critical Industry Academic Cooperation Foundation of Yonsei University
Publication of WO2021172811A1 publication Critical patent/WO2021172811A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/36—Means for collection or storage of gas; Gas holders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/107—Apparatus for enzymology or microbiology with means for collecting fermentation gases, e.g. methane
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/14—Pressurized fluid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas

Definitions

  • the present invention relates to a culture apparatus and a culture system that provide an environment similar to the human body including a cell culture unit, and more particularly, to a human body including a cell culture unit that provides tension and compressive force acting on specific cells inside the human body It relates to a culture device and a culture system that provide an environment similar to that of
  • the cells are grown using a liquid or gel-type nutrient medium containing nutrients that can grow cells, and drugs are injected into the grown cells to observe the cells.
  • the culture device applies tension and compression together, creates an environment similar to the inside of the human body together with tension and compression, and means to confirm that the drug is applied in an environment similar to the inside of the human body is actively developed. It is not, and a method to solve these problems is required.
  • the present invention is an invention devised to solve the problems of the prior art described above, and as air is injected and the elastic culture part expands, it pulls the cells in all directions to provide tension, and the cells are pressed by the elasticity of the compression part while the compressive force is provided.
  • the matrix member can create an environment similar to the extracellular matrix between the elastic culture part and the compression part, and the medium moves along the micropores of the matrix member, thereby creating an environment similar to human blood circulation. .
  • the cell culture unit of the present invention for achieving the above object is a cell culture unit for creating an environment in which tension and compressive force are provided to specific cells inside the human body, and a tension providing unit in which an internal space open upward is formed. , a supply part communicating with the bottom surface of the tension providing part so that air moves toward the internal space, is inserted into the internal space to seal a part of the internal space, and the cells are located at the top so that air is injected from the supply part, the tension It includes an elastic culture unit that expands toward the upper portion of the donor and a compression unit positioned on the elastic culture unit to limit the expansion of the elastic culture unit, wherein the elastic culture unit expands toward the compression unit around the cell. It is characterized in that the tension is applied toward the.
  • the compression unit is characterized in that the elastic culture unit is deformed toward the upper portion of the tension providing unit as the elastic culture unit expands and pressurizes so that a compressive force is applied to the cells.
  • it is characterized in that it includes a support part inserted into the compression part in order to fix the compression part.
  • the support part is characterized in that it fixes the inner edge of the compression part so that the compression part can be deformed into a hemispherical shape.
  • the support portion characterized in that it includes a packing member formed around the inserted support portion to prevent separation from the tension providing portion.
  • the compression unit characterized in that it comprises a substrate member provided between the elastic culture unit and the compression unit to prevent the cells from being damaged by pressure.
  • the substrate member is characterized in that pores are formed so that a medium or oxygen can be supplied to the cells.
  • the tension providing unit is composed of an outer tube and an inner tube, characterized in that the inner tube is spaced apart from the outer tube a predetermined distance.
  • the tension providing unit is characterized in that the side wall of the elastic culture portion is inserted between the outer tube and the inner tube to fix the elastic culture portion.
  • the supply unit is characterized in that it is provided with a third valve that blocks the movement of the air according to the rotation in order to control the air moving toward the inner space.
  • the culture apparatus for providing an environment similar to the human body of the present invention for achieving the above object is a tension providing part in which an internal space open toward the top is formed, the bottom surface of the tension providing part so that air moves toward the internal space
  • the supply unit communicating with the inner space is inserted into the inner space to seal a part of the inner space, and the cells are located on the upper part, and the elastic culture part expands toward the upper part of the tension providing part while air is injected from the supply part and the elastic culture part on the upper part.
  • a cell culture unit and the cell culture unit characterized in that the cell culture unit and the cell culture unit, characterized in that the cell culture unit and the cell culture unit, characterized in that it is positioned to limit the expansion of the elastic culture part, the elastic culture part is expanded toward the compression part, and tension is applied to the cell toward the periphery of the elastic culture part. It communicates with the unit and includes a circulation unit in which the medium is moved.
  • the circulation unit is characterized in that it comprises an inlet through which the medium is moved toward the cell culture unit and an outlet through which the medium passing through the cell culture unit is discharged.
  • the circulation unit is provided in the inlet, a first valve to block the movement of the medium in order to control the flow of the medium, and a second valve to change the movement path of the medium to extract the sample from the outlet It is characterized in that it includes a valve.
  • the cell culture unit is characterized in that the substrate member is provided between the elastic culture unit and the compression unit so that the medium or the drug supplied through the inlet can be supplied to the cells.
  • the substrate member characterized in that the pores are formed so that the medium or the drug supplied through the circulation unit can be moved to the cells.
  • the culture system of the present invention for achieving the above object is a housing having a space formed therein, a tension providing unit positioned inside the housing to form an internal space open toward the top, and sealing a part of the internal space. Inserted into the inner space, the cells are positioned at the upper portion, and an elastic culture unit that expands toward the upper portion of the tension providing unit while air is injected into the inner space, and a compression unit positioned above the elastic culture unit to limit the expansion of the elastic culture unit Including, wherein the elastic culture unit expands toward the compression unit, and a plurality of cell culture units, characterized in that tension is applied toward the periphery of the elastic culture unit on the cells, and the plurality of the cells to move air toward the inner space. It includes a plurality of supply units in communication with the bottom surface of the cell culture unit.
  • the supply unit is characterized in that a plurality of them have the same length.
  • the supply unit is characterized in that it controls the amount of air moved to at least one or more of the cell culture unit.
  • a culture apparatus and a culture system that provide an environment similar to a human body including the cell culture unit of the present invention for solving the above problems have the following effects.
  • the tension applied inside the human body is applied to create a situation similar to the inside of the human body in the cells.
  • the cells are provided with the compressive force, thereby creating a situation similar to the inside of the human body.
  • a matrix member is provided between the elastic culture unit and the compression unit, thereby creating an environment similar to the extracellular matrix inside the human body.
  • FIG. 1 is a view showing the overall appearance of a culture apparatus and a culture system that provides an environment similar to a human body including a cell culture unit according to an embodiment of the present invention
  • FIG. 2 is a view showing a cross-section of a cell culture unit of a culture apparatus and a culture system that provides an environment similar to a human body including a cell culture unit according to an embodiment of the present invention
  • FIG. 3 is a view partially showing a combined perspective view of a culture device and a culture system that provide an environment similar to a human body including a cell culture unit according to an embodiment of the present invention
  • Figure 4 is a view showing a combined perspective view of the compression part and the support part of the culture apparatus and culture system that provides an environment similar to the human body including a cell culture unit according to an embodiment of the present invention
  • FIG. 5 is a view showing a situation in which air is injected into the elastic culture unit of the culture apparatus and culture system that provides an environment similar to the human body including the cell culture unit according to an embodiment of the present invention
  • Figure 6 is a view showing a situation in which the compressive force is applied to the cells of the culture apparatus and culture system that provides an environment similar to the human body including the cell culture unit according to an embodiment of the present invention
  • Figure 7 is a view showing a situation in which the medium of the culture device and culture system to provide an environment similar to the human body including the cell culture unit according to an embodiment of the present invention is moved;
  • FIG. 8 is a view showing a situation in which the medium of the culture apparatus and the culture system that provides an environment similar to the human body including the cell culture unit according to an embodiment of the present invention is moved as viewed from the top;
  • FIG. 9 is a diagram illustrating a culture system of a culture apparatus and a culture system that provides an environment similar to a human body including a cell culture unit according to an embodiment of the present invention.
  • FIGS. 1 to 4 a culture device that provides an environment similar to a human body including the cell culture unit 100 according to an embodiment of the present invention may be described with reference to FIGS. 1 to 4 .
  • FIG. 1 is a view showing the overall appearance of a culture apparatus and a culture system that provide an environment similar to a human body including a cell culture unit according to an embodiment of the present invention
  • FIG. 2 is a view showing an embodiment of the present invention
  • the above-described culture unit may be composed of a cell culture unit 100 and a circulation unit 200 as shown in FIG. 1 .
  • the cell culture unit 100 will be described in more detail through the description of FIGS. 2 to 4 described above, and the circulation unit 200 is directed toward the cell culture unit 100 as shown in FIG. 1 . It may be composed of an inlet 220 through which the medium is moved and an outlet 240 through which the medium passing through the cell culture unit 100 is discharged.
  • the inlet 220 may be provided with a first valve 222 for blocking the movement of the medium or drug according to the rotation, the discharge unit 240 according to the rotation path of the medium or the drug
  • a second valve 242 for changing may be provided.
  • the medium may be moved toward the cell culture unit 100 through the inlet 220 to supply nutrients to the cells C disposed in the cell culture unit 100 .
  • the first valve 222 blocks the movement of the medium to the cell culture unit 100 while rotating, and the drug is transferred through the inlet 220 and delivered to the cells C can make it happen
  • the second valve 242 changes the movement path of the medium while rotating so that the secretion of the cells (C) injected with the drug or a portion of a large amount of the cells (C) can be collected. have.
  • the sample including the drug and the cell (C) or secretion of the cell (C) extracted through the second valve 242, a large amount of the cell (C), etc. can be arbitrarily changed by the user.
  • the type of the sample may vary depending on circumstances, it is specified that the drug and the sample may vary according to the intention and situation of the user.
  • a tension providing unit 110 a supply unit 120 , an elastic culture unit 130 , and compression
  • the part 140 , the substrate member 142 , the support part 150 , and the packing member 152 may be provided.
  • the tension providing unit 110 has an internal space open upwards, and the supply unit 120 is the tension providing unit 110 to inject air toward the internal space of the tension providing unit 110 . ) can be connected to the bottom surface of
  • the elastic culture unit 130 may be inserted into the inner space to seal a part of the inner space, and the cells (C) may be located thereon.
  • the compression unit 140 is located above the elastic culture unit 130, air is injected toward the inner space sealed by the elastic culture unit 130, the elastic culture unit 130 is the tension agent.
  • the compression unit 140 having elasticity is deformed toward the upper portion of the tension providing unit 110 in response to the expansion of the elastic culture unit 130, and the elastic culture unit
  • the force to return to the state before being deformed by the expansion of 130 may act to pressurize the cell (C).
  • the support part 150 is inserted into the compression part 140 to fix the inner edge of the compression part 140 so that the compression part 140 can be deformed toward the upper part of the tension providing part 110, and the support part 150 is provided.
  • the packing member 152 provided along the circumference of the support part 150 at the upper part of the tension providing part 110 is provided. can be formed.
  • the packing member 152 may be in close contact with the tension providing part 110 between the tension providing part 110 and the support part 150 so that the support part 150 is fixed.
  • the packing member 152 as described above allows the tension providing part 110 and the support part 150 to be in close contact with each other, and by improving the frictional force between the tension providing part 110 and the support part 150 , the support part It is possible to prevent the 150 from being separated from the tension providing unit 110 .
  • the tension providing unit 110 includes an outer wall 112 and an inner wall 114 , and the inner wall 114 is The outer wall 112 and the inner space may be formed to be spaced apart from each other by a predetermined distance.
  • the supply unit 120 communicating with the bottom surface of the tension providing unit 110 is provided with a third valve 122 that blocks the flow of air according to rotation in order to control the flow of air. It is possible to adjust the amount of air moved to (110).
  • the elastic culturing part 130 is inserted into the tension providing part 110 , and the sidewall of the elastic culturing part 130 is inserted into the gap between the outer wall 112 and the inner wall 114 .
  • the inner wall 114 and the elastic culture unit 130 form a closed space in the inner space, and the side wall of the elastic culture unit 130 is in close contact with the outer wall 112 and the inner wall 114 and fixed can be
  • a 142 may be provided between the elastic culture unit 130 and the compression unit 140 .
  • the substrate member 142 may be formed of a sponge material or a gel-like material to prevent the cells (C) from being damaged, but it is not toxic to the cells (C), and the A material in which micropores are formed so that the medium can be moved through the circulation unit 220 may be more efficient.
  • the matrix member 142 is not toxic to the cell (C), and since micropores must be formed so that the medium can be moved, an environment similar to the extracellular matrix of the cell (C) can be created. No toxicity acts on the cell (C) using alginate, a material that can environment can be created.
  • the upper portion of the elastic culture unit 130 is the tension providing unit ( 110) is expanded in a hemispherical shape toward the upper portion, and the compression unit 140 may be deformed toward the upper portion of the tension providing unit 110 as the elastic culture unit 130 expands.
  • the support part 150 is the It may be inserted into the compression unit 140 to support the inner edge of the compression unit 140 .
  • the support unit 150 may be formed with a medium passage for preventing the medium from overflowing by the elastic culture unit 130 and the compression unit 140 .
  • FIG. 5 is a view showing a situation in which air is injected inside the elastic culture unit of a culture device and a culture system that provides an environment similar to a human body including a cell culture unit according to an embodiment of the present invention
  • FIG. 6 is 7 is a view showing a situation in which a compressive force is applied to cells of a culture apparatus and a culture system that provide an environment similar to a human body including a cell culture unit according to an embodiment of the present invention
  • 8 is a diagram showing a situation in which a culture device and a culture system providing an environment similar to the human body including the cell culture unit are moved and FIG. 8 is an environment similar to the human body including the cell culture unit according to an embodiment of the present invention It is a view showing a situation in which the culture equipment and culture system that provide the medium are moved as viewed from the top.
  • the elastic culture unit 130 that can be stretched is the upper part. It expands toward a hemispherical shape, and tension may be applied while the cells (C) attached to the upper portion of the elastic culture unit 130 are pulled toward the circumference of the elastic culture unit 130 .
  • the compression part 140 positioned on the elastic culturing part 130 expands the elastic culturing part 130 while the tension providing part 110 .
  • Pressure may be applied to the cell (C) by a force that is deformed upward and returns to a position before deformation.
  • the substrate member 142 is disposed between the compression unit 140 and the elastic culture unit 130 as described above. ) may be provided.
  • the substrate member 142 has micropores formed therein so that the medium and oxygen can move so that the cells C can grow, and the drug injected through the inlet 220 and It can act on the cell (C) together.
  • the medium is moved between the compression unit 140 and the elastic culture unit 130 through the micropores formed in the substrate member 142 and the cells (C) is delivered to, and may be moved toward the discharge unit 240 along the micropores of the substrate member 142 .
  • the cell (C) is the tension applied inside the human body as the elastic culture unit 130 expands, the tension is applied, and the compression force is pressurized by the force that the compression unit 140 is restored to. and a compressive force is applied, and an environment similar to the extracellular matrix is created by the matrix member 142, so that it can grow in an environment more similar to that of the human body.
  • the drug when the drug is injected into the cell (C), when directly injected into the cell (C), or when the drug is put into the cell (C) and applied, the drug passes through the blood vessel inside the human body Similar to the injection of the drug, since it acts on the cells C through the micropores of the matrix member 142, the effect of the drug in the human body can be confirmed more similarly.
  • the cell culture units 100 When a plurality of the cell culture units 100 are used to culture the cells C using the cell culture unit 100 described above, there is a space inside so that the cell culture units 100 can be arranged.
  • the housing in which this is formed and the supply unit S in which air is moved toward the cell culture unit 100 may be provided.
  • the supply unit (S) may be formed as a passage divided to be connected to the bottom surface of each of the plurality of the cell culture units (100), and the supply unit (S) is a plurality of the cell culture units (100), respectively It may be formed to have the same length as
  • the at least one supply unit (S) connected to the cell culture unit (100) is divided at least once to communicate with the cell culture unit (100), and the divided supply unit (S) is Each length may be the same from the initially divided position to the plurality of cell culture units 100 .

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Abstract

L'invention concerne un incubateur et un système de culture pour fournir un environnement similaire au corps humain, comprenant une unité de culture cellulaire, selon la présente invention, fournissant les éléments suivants : une unité de culture cellulaire pour créer un environnement interne du corps humain dans lequel une tension et une force de compression sont fournies à des cellules spécifiques ; et un incubateur et un système de culture comprenant l'unité de culture cellulaire. L'unité de culture cellulaire comprend les éléments suivants : une partie de fourniture de tension ayant un espace intérieur ouvert vers le haut formé en son sein ; une partie d'alimentation communiquant avec la surface inférieure de la partie de fourniture de tension de telle sorte que de l'air peut circuler vers l'espace interne ; une partie de culture élastique étant insérée dans l'espace interne de façon à sceller une partie de l'espace interne, ayant des cellules positionnées sur sa partie supérieure, et se dilatant vers la partie supérieure de la partie de fourniture de tension en ayant de l'air lui étant injecté à partir de la partie d'alimentation; et une partie de compression étant positionnée sur la partie supérieure de la partie de culture élastique de façon à limiter l'expansion de la partie de culture élastique, de sorte que lorsque la partie de culture élastique se dilate vers la partie de compression, une tension agit sur les cellules vers la circonférence de la partie de culture élastique
PCT/KR2021/002072 2020-02-27 2021-02-18 Incubateur et système de culture pour fournir un environnement similaire au corps humain, comprenant une unité de culture cellulaire Ceased WO2021172811A1 (fr)

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KR1020200024279A KR102375692B1 (ko) 2020-02-27 2020-02-27 세포배양유닛을 포함하는 인체와 유사한 환경을 제공하는 배양기기 및 배양시스템
KR10-2020-0024279 2020-02-27

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US20180164278A1 (en) * 2015-06-09 2018-06-14 Technische Universität Darmstadt Testing arrangement for examining a cell culture under the effect of a dynamic force
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KR101569619B1 (ko) * 2014-05-02 2015-11-17 인하대학교 산학협력단 수축 및 팽창 기능을 하는 인체장기를 모사한 실험모델장치

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KR101269368B1 (ko) * 2006-07-10 2013-05-29 더 브리검 앤드 우먼즈 하스피털, 인크. 세포 또는 조직의 배양 방법
KR20120014137A (ko) * 2009-04-03 2012-02-16 크셀렉스, 인크. 조직 및 장기 이식편 생물반응기 및 작동 방법
JP6047000B2 (ja) * 2012-12-12 2016-12-21 日本メナード化粧品株式会社 細胞を含む支持体層に培養液を灌流させることを特徴とする３次元培養組織
US20180164278A1 (en) * 2015-06-09 2018-06-14 Technische Universität Darmstadt Testing arrangement for examining a cell culture under the effect of a dynamic force
WO2019025070A1 (fr) * 2017-07-31 2019-02-07 Università Degli Studi Di Genova Échafaudage d'hydrogel tridimensionnel pour culture cellulaire et sa méthode de production

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