WO2021022562A1 - Pure natural compound essential oil having broad-spectrum bactericidal activity and preparation and application thereof - Google Patents

Abstract

Preparation and application of a pure natural compound essential oil having broad-spectrum bactericidal activity. The compound essential oil is obtained by extracting volatile oils from dry roots and stems of Atractylodes lancea (Thunb.) DC. or Atractylodes chinensis (DC.) Koidz. which belong to atractylodes plants of the Compositae family as well as dry twigs or bark of cinnamonum cassia Presl. which belongs to Lauraceae plants, and then mixing and compounding the volatile oils, the volume content of the two volatile oils being: 40-70 parts atractylode volatile oil and 30-60 parts of cassia twig volatile oil. The compound essential oil may have a strong killing effect on Staphylococcus aureus, Escherichia coli and Candida albicans.

Description

Pure natural compound essential oil with broad-spectrum bactericidal activity and preparation and use thereof Technical field

The invention belongs to the technical field of sterilization, disinfection and antiseptic, and specifically relates to pure natural compound essential oils, preparation methods thereof, and uses in medical drugs, sanitary disinfection products, food antiseptics and daily necessities.

Background technique

Rhizoma Atractylodis, also known as Atractylodis sinensis, is the dry rhizome of Atractylodes lancea (Thunb.) DC. or Atractylodeschinensis (DC.) Koidz. The main function is pungent and warm, specializing in the spleen and stomach, drying dampness and strengthening the spleen, dispelling wind and removing dampness. Using atractylodes to fumigate the air has the effect of relieving depression and avoiding filth, and its main bactericidal active ingredient is volatile oil. The fumigation and disinfection of atractylodes has been used in wards, delivery rooms, and operating rooms to achieve satisfactory results. Cassia twig is a dry twig of Cinnamonum cassia Presl., a plant of the family Lauraceae. It has the effects of sweating and relieving muscles, warming the meridians, promoting yang and qi, and calming down the qi. Osmanthus fragrans volatile oil is the volatile component of Osmanthus fragrans and is the main active substance of Osmanthus fragrans. Modern research has found that the volatile oil of Osmanthus fragrans is the main effective part of Osmanthus fragrans and has good anti-inflammatory, antibacterial, antiviral and antitumor effects. The bactericidal activity of the volatile oil of Atractylodes Rhizome and Cinnamomum cassia volatile oil alone has been reported in the literature, but the bactericidal activity, extraction and preparation method and use of the compound essential oil prepared from the volatile oil of Atractylodes Rhizome and Cinnamomum cassia have no related literature and patents. Single atractylodes volatile oil or cassia twig volatile oil cannot simultaneously kill Staphylococcus aureus, Escherichia coli and Candida albicans and make the final sterilization effect reach the national sanitary disinfection standard of 5 logarithms, while the pure natural compound essential oil of the present invention can exert both The synergistic effect of these essential oils can kill Staphylococcus aureus, Escherichia coli and Candida albicans at the same time and make the final sterilization effect reach and exceed the national sanitary disinfection standard of 5 logarithms.

Summary of the invention

The purpose of the present invention is to provide a preparation and application of a pure natural compound essential oil with broad-spectrum antibacterial activity. The compound essential oil is obtained from the dry roots and stems of Atractylodes lanceolata or Atractylodes lanceolata, and the dry tenderness of Cinnamomum cassia The volatile oil is extracted from the branches or bark and then mixed and compounded.

In specific cases, the volume content of the two volatile oils in the compound essential oil is: Atractylodes lancea volatile oil: 40-70 parts, and Guizhi volatile oil: 30-60 parts.

Further preferably, the volume content of the two volatile oils is: atractylodes volatile oil: 50-60 parts, and Guizhi volatile oil: 40-50 parts.

Atractylodes volatile oil mainly contains volatile substances such as β-cineole, atractylyl alcohol, atractylone, atractyl alcohol, elemene alcohol and so on. It can not only kill Mycobacterium tuberculosis and Pseudomonas aeruginosa in the air to a certain extent, but also has a strong killing effect on Escherichia coli and Staphylococcus aureus. Cinnamon stick oil mainly contains volatile substances such as benzaldehyde, phenylpropanal, trans-cinnamaldehyde, and cinnamaldehyde, and has significant antibacterial, sedative and analgesic effects. Cangzhu and Guizhi are commonly used Chinese medicines, and their effects have long been recorded in ancient times. However, the inventor discovered for the first time in a large number of experiments that when the essential oils with the same characteristics are proportioned in an appropriate ratio, they will produce a synergistic effect and double the bactericidal efficacy, that is, the effect of 1+1 is greater than 2. Therefore, the inventor On the basis of fully digging out the various characteristics and effects of medicinal plants, the pure natural antiseptic compound essential oil of Atractylodes Rhizome and Cassia twig volatile oil was designed through the main and auxiliary collocation.

The compound essential oil has a synergistic effect on the bactericidal effect of the main active ingredient in atractylodes volatile oil and the main active ingredient in cinnamon twig volatile oil, cinnamaldehyde, etc. The two are used in combination to reduce the dosage and can treat Staphylococcus aureus, Escherichia coli and Candida albicans. Bacteria has obvious killing effect, the mechanism is that atractylol and cinnamaldehyde synergistically inhibit bacterial cell wall synthesis, cell wall mucopeptide synthase (including transpeptidase, carboxyfetozyme, endopeptidase), thereby hindering the synthesis of cell wall peptidoglycan , So that the cell wall is defective, the bacteria rupture and die.

The compound essential oil of the present invention can be prepared by steam distillation, which specifically includes the following steps:

(1) Extraction of atractylodes rhizome oil: crush the rhizome of atractylodes japonicus into dry powder, put it into a steam distillation extractor containing 10 times the amount of water, control the temperature at around 100℃ (±5℃), and wait for the liquid flowing down the branch pipe of the extractor When it is colorless, stop the extraction, use a separatory funnel to remove the distilled water from the bottom to obtain an oily crude extract, seal it and store it in a refrigerator at 4°C for later use;

(2) Cinnamomum cassia oil extraction: Cinnamomum cassia twig oil is pulverized into dry powder and put into a steam distillation extractor containing 12 times the amount of water. The temperature is controlled at around 100°C (±5°C). When the liquid is colorless, stop the extraction and use a separatory funnel to remove the distilled water from the bottom to obtain an oily crude extract, which is sealed and stored in a refrigerator at 4°C for later use;

(3) Mixing atractylodes oil and cassia twig oil in a certain ratio to obtain a pure natural compound essential oil with broad-spectrum antibacterial activity.

In addition, the volatile oil of Cangzhu and Guizhi in the present invention can also be prepared by CO ₂ supercritical (or subcritical) fluid extraction, which specifically includes the following steps:

(1) Extraction of atractylodes rhizome: pulverize the rhizome of atractylodes japonicus into dry powder, put it in a CO ₂ supercritical fluid extraction tank of appropriate size, and directly extract with supercritical CO ₂ to obtain;

(2) Cassia twig oil extraction: Cinnamon twig is crushed into dry powder, placed in a CO ₂ supercritical fluid extraction tank of appropriate size, and directly extracted with supercritical CO ₂ to obtain;

(3) Mixing atractylodes oil and cassia twig oil in a certain ratio to obtain a pure natural compound essential oil with broad-spectrum antibacterial activity.

According to a preferred aspect of the present invention, there is also provided a three-component compound essential oil and a preparation method thereof, the method comprising:

(1) Extraction of atractylodes rhizome oil: crush the rhizome of atractylodes japonicus into dry powder, put it into a steam distillation extractor containing 10 times the amount of water, control the temperature at around 100℃ (±5℃), and wait for the liquid flowing down the branch pipe of the extractor When it is colorless, stop the extraction, use a separatory funnel to remove the distilled water from the bottom to obtain an oily crude extract, seal it and store it in a refrigerator at 4°C for later use;

(2) Cinnamomum cassia oil extraction: Cinnamomum cassia twig oil is pulverized into dry powder and put into a steam distillation extractor containing 12 times the amount of water. The temperature is controlled at around 100°C (±5°C). When the liquid is colorless, stop the extraction, use a separatory funnel to remove the distilled water from the bottom to obtain an oily crude extract, seal it and store it in a refrigerator at 4°C for later use;

(3) Extraction process of medicinal koji grass oil: pulverize medicinal koji grass into dry powder, and put it into a steam distillation extractor containing 15 times the amount of water. The temperature shall be controlled at around 100°C (±5°C). When the liquid flowing down is colorless, stop the extraction. Use a separatory funnel to remove the distilled water from the bottom to obtain an oily crude extract, which is sealed and stored in a refrigerator at 4°C for later use.

The atractylodes oil and medicinal koji oil prepared above are mixed in a volume ratio of 10:1 to 10:3, preferably a volume ratio of 10:2, to form a first mixed oil.

The first mixed oil is heated in a water bath at 60-80°C, preferably 70°C for 10 to 30 minutes, and then cinnamon twig oil is added to form a second mixed oil. The volume ratio of the added cinnamon twig oil to the first mixed liquid is 1:3 to Between 3:1, preferably 1:2.

Then the second mixed oil is heated at 50-60°C, preferably 55°C in a water bath for 5 to 10 minutes, thereby preparing an improved three-component compound essential oil.

The inventor unexpectedly discovered during the compatibility test that the medicinal koji oil added in the above process steps can significantly improve the sterilization effect of the volatile oil of Atractylodes Rhizome and Cassia twig, which may be related to the synergistic effect brought by its permeability and may also be its auxiliary Sterilization performance is also related.

The present invention conducts sterilization experiments on the compound essential oils (including two-component and three-component compound essential oils, the same below), and shows that they have strong bactericidal activity against Staphylococcus aureus, Escherichia coli, Candida albicans, etc.

The compound essential oil in the present invention can be used alone, and can be used to prepare medicines (including human and animal medicines) for treating or relieving bacterial or unexplained inflammation, fever, pain and other symptoms.

The compound essential oil of the present invention can also be compatible with other Chinese medicines with antibacterial, anti-inflammatory, antipyretic and analgesic effects, such as honeysuckle, scutellaria, forsythia, dandelion, etc., or western medicine penicillin, roxithromycin, cephalosporin, etc. Oral, injection and other internal preparations with antibacterial, anti-inflammatory, antipyretic and analgesic effects, or external preparations such as suppositories, ointments, sprays, drops, etc., used to treat and alleviate inflammation caused by various infectious diseases or unknown swelling Symptoms such as fever and pain.

The compound essential oil in the present invention can also be used for the development of sterilizing daily necessities.

The compound essential oil of the present invention can also be added with some surfactants and auxiliary materials, such as TEA sodium lauryl sulfate, cocamide DEA, cocamide MEA, sodium laureth sulfate, etc. The development includes disinfectant, disinfectant Daily products such as towels, oral antibacterial sprays, clothing disinfectants, antiseptic toothpastes, antibacterial shower gels, etc.

The compound essential oil in the present invention can also be used as a pure natural antiseptic preservative in food and health food.

The compound essential oil of the present invention can also be used for the development of pure natural sterilization products for poultry, livestock, aquaculture and the like.

The invention has wide raw material sources, low cost, low toxicity, simple and universal preparation method, and the product has broad-spectrum antibacterial, anti-inflammatory, antipyretic and analgesic effects.

detailed description

1. Preparation of compound essential oil

Preparation process (1)

Atractylodes lanceolata oil extraction process: pulverize the rhizome of atractylodes lanceolata into dry powder, put it into a steam distillation extractor containing 10 times the amount of water, and control the temperature at around 100℃. When the liquid flowing down the branch pipe of the extractor is colorless, about 6h , Stop the extraction, use a separatory funnel to remove the distilled water from the bottom to obtain the crude oily extract, seal it and store it in a refrigerator at 4°C for later use.

Cinnamomum cassia oil extraction process: pulverize cassia twig into dry powder, and put it in a steam distillation extractor containing 12 times the amount of water. The temperature is controlled at around 100℃. When the liquid flowing down the branch pipe of the extractor is colorless, it will be approximately 6h, stop the extraction, use a separatory funnel to remove the distilled water from the bottom to obtain an oily crude extract, seal it and store it in a refrigerator at 4°C for later use.

The atractylodes oil and the cassia twig oil are mixed in a certain ratio to obtain a two-component pure natural compound essential oil with broad-spectrum antibacterial activity.

Preparation process (2)

The extraction process of medicinal koji grass oil: crush medicinal koji grass (tougucao) into dry powder and put it into a steam distillation extractor containing 15 times the amount of water. The temperature shall be controlled at around 100°C. When the liquid is colorless, the extraction is stopped for about 6 hours, and the distilled water at the bottom is removed with a separatory funnel to obtain an oily crude extract, which is sealed and stored in a refrigerator at 4°C for later use.

The atractylodes oil and medicinal koji oil prepared above are mixed in a volume ratio of 10:1 to 10:3, preferably a volume ratio of 10:2, to form a first mixed oil.

The first mixed oil is heated in a water bath at 60-80°C, preferably 70°C for 10 to 30 minutes, and then cinnamon twig oil is added to form a second mixed oil. The volume ratio of the added cinnamon twig oil to the first mixed liquid is 1:3 to Between 3:1, preferably 1:2.

Then the second mixed oil is heated in a water bath at 50-60°C, preferably 55°C for 5-10 minutes, thereby preparing an improved three-component pure natural compound essential oil with broad-spectrum antibacterial activity.

2. Sterilization test

Experiment 1

(1) Three strains of Staphylococcus aureus, Escherichia coli, and Candida albicans were selected to prepare experimental bacterial suspensions according to the disinfection technical specifications, and the concentration was 1×108cfu/ml～5×108cfu/ml.

(2) Dissolve 0.2589 g of compound essential oils with different mixing ratios in 5 mL of ethanol and dilute the volume to 25 mL with distilled water to prepare a disinfectant with a concentration of 1.0%.

(3) Aseptic test tube for detoxification test, first add 0.5ml test bacteria suspension, then add 0.5ml organic interfering substance, mix well, put it in a water bath at 20℃±1℃ for 5 minutes, then use a sterile straw Inject 4.0ml of liquid into it, mix quickly and record the time immediately.

(4) After the test bacteria interact with the disinfectant solution for each predetermined time, respectively add 0.5ml of the test bacteria and the disinfectant mixture to 4.5ml of the sterilized neutralizer, and mix well.

(5) After each tube of the test bacteria and disinfectant mixture is added with a neutralizer for 10 minutes, absorb 1.0ml of the sample solution, and determine the number of viable bacteria according to the method of living bacteria culture and counting. Each tube of sample solution can be inoculated with 2 plates. If there are a large number of colonies growing on the plate, a series of 10-fold dilutions can be carried out, and then live bacterial culture and counting can be carried out.

(6) At the same time, use the diluent instead of the disinfectant, and conduct parallel tests as a positive control.

(7) All test samples were cultured in a 37°C incubator. The bacterial propagules were cultured for 48 hours to observe the final results; bacterial spores were cultured for 72 hours to observe the final results.

(8) The experiment was repeated 3 times, the concentration of viable bacteria (cfu/ml) of each group was calculated and converted to logarithmic value (N), and then the logarithmic killing value was calculated as follows:

Killing logarithmic value (KL) = logarithm of the average viable bacteria concentration of the control group (No)-logarithmic value of the viable bacteria concentration of the test group (Nx)

The test results are shown in Table 1.

Table 1: Suspension quantitative sterilization test results

The results of the above sterilization test show that:

The use of atractylodes oil and cinnamon twig oil alone has a strong killing effect on Staphylococcus aureus, with a log killing value (KL)>5.0, but the killing log value of Escherichia coli and Candida albicans cannot reach the use effect. After the two are used together, it can be seen from the screening results of Examples 3-6 that the main active ingredients in the volatile oil of Atractylodes Rhizome and the volatile oil of Guizhi have a synergistic effect. While reducing the dosage, it has obvious effects on Staphylococcus aureus, Escherichia coli and Candida albicans. The comprehensive average logarithmic value of killing was 5.91, 5.75, 5.76 and 5.85 respectively.

Test 2

The two-component compound essential oil prepared by the above preparation process (1) and the three-component compound essential oil prepared by the preparation process (2) were subjected to a sterilization test according to the standard of the above test 1. Add, the test results are shown in Table 2 below.

Table 2: Suspension quantitative sterilization test results

The results of the sterilization test in Table 2 show:

The bactericidal effect of the improved three-component compound essential oil prepared by the preparation process (2) is significantly enhanced, which is related to the fact that the bactericidal effect of the two is further promoted by the medicinal koji oil added in the above specific process steps.

Three, specific application examples

3.1 Application examples of compound essential oils in medicines

Example 1 Preparation of two-component compound essential oil soft capsules

Formula (weight ratio): two-component compound essential oil 5.8%, soft capsule base: gelatin 17.7%, agar 17.7%, deionized water 58.8%.

Preparation method: After placing the above soft capsule matrix in a gelatinization tank to prepare a glue solution, press it into a soft capsule with a soft capsule machine, and control the weight of each pill to be 0.5g to obtain 1000 two-component compound essential oil capsules.

Example 2 Preparation of two-component compound essential oil suppository

Formula (weight ratio): two-component compound essential oil 5.9%, borneol 1.2%, suppository base: polyoxyethylene stearate 88.5%, polysorbate 80 4.4%.

Preparation method: first dissolve borneol with an appropriate amount of ethanol, and then mix it thoroughly with the two-component compound essential oil and polysorbate 80. In addition, the polyoxyethylene stearate is melted on a water bath, the above-mentioned medicinal solution is added, and the mixture is thoroughly stirred, poured into the suppository mold, and taken out after cooling to make 1000 tablets, which is ready.

Example 3 Preparation of modified three-component compound essential oil soft capsule

Formula (weight ratio): three-component compound essential oil 5.8%, soft capsule base: gelatin 17.7%, agar 17.7%, deionized water 58.8%.

Preparation method: After placing the above soft capsule matrix in a gelatinizing tank to prepare a glue solution, press it into a soft capsule with a soft capsule machine, and control the weight of each pellet to 0.5g to obtain 1,000 three-component compound essential oil capsules.

The above soft capsules and suppositories can be used to treat and alleviate bacterial or unexplained urinary tract infections, nephritis, cystitis, vaginitis, mastitis, tonsillitis, pneumonia, encephalitis, mumps, upper respiratory tract infections and other diseases , Fever, pain and other symptoms, have good application prospects.

3.2 Application examples of compound essential oils in oral sterilization sprays

A pure natural oral antiseptic spray, consisting of the following components by weight percentage:

Atractylodes japonicus volatile oil: 1.0%-1.5%, Guizhi volatile oil: 0.8%-1.2%, cocamide DEA: 0.5%-1.0%, sodium laureth sulfate: 3%-4%, TEA sodium lauryl sulfate: 2.0%-3.0%, glycerin: 5%-8%, stevia: 5%-8%, borneol: 0.2%-0.3%, food flavor: 0.3%-0.5%, purified water: the balance.

An enhanced oral sterilization spray is also provided, which is composed of the following components by weight percentage: atractylodes volatile oil: 1.0%-1.5%, cassia twig volatile oil: 0.8%-1.2%, medicinal koji oil ：0.1-0.3%, Cocamide DEA: 0.5%-1.0%, Sodium Laureth Sulfate: 3%-4%, TEA Sodium Lauryl Sulfate: 2.0%-3.0%, Glycerin: 5%-8% , Stevia: 5%-8%, borneol: 0.2%-0.3%, food flavor: 0.3%-0.5%, purified water: balance.

Preparation process: Mix purified water evenly, add cocamide DEA, sodium lauryl ether sulfate, TEA sodium lauryl sulfate, soda, baking soda, glycerin, stevia, edible flavor, and stir to make it fully dissolved. Add atractylodes oil, cinnamon twig oil and medicinal koji oil, the specific added ingredients are operated according to the following specific experimental examples, mechanical stirring at room temperature for 20 minutes, stirring speed 200 rpm, so that it is fully dissolved to a transparent and clear state Then add borneol and fill it up after it is fully dissolved.

Example 4

Atractylodes japonicus volatile oil: 1.0%, cinnamon twig volatile oil: 0.8%, cocamide DEA: 0.5%, sodium laureth sulfate: 3%, TEA sodium lauryl sulfate: 2.0%, glycerin: 5%, stevioside: 5 %, borneol: 0.2%, food flavor: 0.3%, purified water: balance.

Example 5

Atractylodes japonicus volatile oil: 1.5%, cinnamon twig volatile oil: 1.2%, cocamide DEA: 1.0%, sodium laureth sulfate: 4%, TEA sodium lauryl sulfate: 3.0%, glycerin: 8%, stevioside: 8 %, borneol: 0.3%, food flavor: 0.5%, purified water: balance.

Example 6

Atractylodes volatile oil: 1.5%, Cassia twig volatile oil: 0.8%, Cocamide DEA: 0.5%, Sodium laureth sulfate: 3%, TEA sodium lauryl sulfate: 2.0%, Glycerin: 5%, Stevia: 5 %, borneol: 0.2%, food flavor: 0.3%, purified water: balance.

Example 7

Atractylodes japonicus volatile oil: 1.0%, cinnamon twig volatile oil: 1.2%, cocamide DEA: 1.0%, sodium laureth sulfate: 4%, TEA sodium lauryl sulfate: 3.0%, glycerin: 8%, stevia: 8 %, borneol: 0.3%, food flavor: 0.5%, purified water: balance.

Example 8

Atractylodes japonicus volatile oil: 1.0%, cinnamon twig volatile oil: 1.0%, cocamide DEA: 0.8%, sodium laureth sulfate: 3.5%, TEA sodium lauryl sulfate: 2.4%, glycerin: 6%, stevioside: 6.5 %, borneol: 0.2%, food flavor: 0.4%, purified water: balance.

Example 9

Atractylodes japonicus volatile oil: 1.1%, cassia twig volatile oil: 0.9%, cocamide DEA: 0.6%, sodium laureth sulfate: 3.8%, TEA sodium lauryl sulfate: 2.6%, glycerin: 7%, stevioside: 7 %, borneol: 0.3%, food flavor: 0.4%, purified water: balance.

Example 10

Atractylodes japonicus volatile oil: 1.0%, cinnamon twig volatile oil: 1.1%, cocamide DEA: 0.8%, sodium laureth sulfate: 3.5%, TEA sodium lauryl sulfate: 2.4%, glycerin: 6%, stevioside: 6.5 %, borneol: 0.2%, food flavor: 0.4%, soda: 0.4%, baking soda 0.2%, purified water: balance.

Example 11

Atractylodes volatile oil: 1.1%, Cassia twig volatile oil: 0.8%, Cocamide DEA: 0.6%, Sodium laureth sulfate: 3.8%, TEA sodium lauryl sulfate: 2.6%, Glycerin: 7%, Stevia: 7 %, borneol: 0.3%, food flavor: 0.4%, soda: 0.5%, baking soda 0.5%, purified water: balance.

Example 12

Atractylodes japonicus volatile oil: 1.0%, cinnamon twig volatile oil: 0.8%, medicinal koji oil 0.1%, cocamide DEA: 0.5%, sodium laureth sulfate: 3%, sodium lauryl sulfate: 2.0%, glycerin: 5%, stevia: 5%, borneol: 0.2%, food flavor: 0.3%, purified water: balance.

Example 13

Atractylodes japonicus volatile oil: 1.5%, cinnamon twig volatile oil: 1.2%, medicinal koji oil 0.1%, cocamide DEA: 1.0%, sodium laureth sulfate: 4%, sodium lauryl sulfate: 3.0%, glycerin: 8%, stevia: 8%, borneol: 0.3%, edible flavor: 0.5%, purified water: balance.

Example 14

Atractylodes volatile oil: 1.5%, Cinnamon twig volatile oil: 0.8%, medicinal koji oil 0.3%, cocamide DEA: 0.5%, sodium laureth sulfate: 3%, sodium lauryl sulfate: 2.0%, glycerin: 5%, stevia: 5%, borneol: 0.2%, food flavor: 0.3%, purified water: balance.

Example 15

Atractylodes japonicus volatile oil: 1.0%, cinnamon twig volatile oil: 1.2%, medicinal koji oil 0.2%, cocamide DEA: 1.0%, sodium laureth sulfate: 4%, sodium lauryl sulfate: 3.0%, glycerin: 8%, stevia: 8%, borneol: 0.3%, edible flavor: 0.5%, purified water: balance.

3.3 Application examples of compound essential oils in disinfection paper towels

A kind of pure natural plant essential oil sterilization and disinfection wipes, which is composed of dust-free paper and wet wipes liquid. The wet wipes liquid is composed of the following raw materials by weight: ethanol: 3%-5%, atractylodes volatile oil: 1.0%-1.5%, Guizhi volatile oil: 0.8%-1.2%, cocamide DEA: 0.5%-1.0%, sodium laureth sulfate: 3%-4%, TEA sodium lauryl sulfate: 2.0%-3.0%, propylene glycol: 5 %-8%, purified water: balance.

According to a preferred aspect of the present invention, there is also provided an enhanced disinfectant wipes, which are composed of the following components by weight percentage: ethanol: 3%-5%, atractylodes volatile oil: 1.0%-1.5%, Gui Branch volatile oil: 0.8%-1.2%, medicinal koji oil 0.1-0.3%, cocoamide DEA: 0.5%-1.0%, sodium laureth sulfate: 3%-4%, sodium lauryl sulfate: 2.0 %-3.0%, propylene glycol: 5%-8%, purified water: balance.

Preparation process: pure water and ethanol are mixed uniformly, and cocamide DEA, sodium laureth sulfate, TEA sodium lauryl sulfate, soda, baking soda, propylene glycol are added separately, and after stirring to make it fully dissolved, add atractylodes oil, The specific ingredients of cassia twig oil and medicinal koji oil were operated according to the following specific experimental examples. Stir mechanically at room temperature for 20 minutes at a stirring speed of 200 rpm to make it fully dissolve to a transparent and clear state, add activated carbon and stir well for adsorption and decolorization. After it is close to colorless, the supernatant is suction filtered to obtain the disinfectant ( Wipes liquid). Soak or spray the disinfectant solution and wood pulp paper towel at a weight of 1:5 to obtain the disinfection paper towel.

The disinfectant was tested with different components and proportions, as follows.

Example 16

Ethanol: 3%, atractylodes volatile oil: 1.0%, cassia twig volatile oil: 0.8%, cocoamide DEA: 0.5%, sodium laureth sulfate: 3%, sodium lauryl sulfate: 2.0%, propylene glycol: 5% , Purified water: margin.

Example 17

Ethanol: 5%, Atractylodes japonicus volatile oil: 1.5%, Cassia twig volatile oil: 1.2%, Cocamide DEA: 1.0%, Sodium laureth sulfate: 4%, TEA sodium lauryl sulfate: 3.0%, Propylene glycol: 5% , Purified water: margin.

Example 18

Ethanol: 4%, Atractylodes volatile oil: 1.5%, Cassia twig volatile oil: 0.8%, Cocamide DEA: 0.5%, Sodium laureth sulfate: 3%, TEA sodium lauryl sulfate: 2.0%, Propylene glycol: 6% , Purified water: margin.

Example 19

Ethanol: 4%, atractylodes volatile oil: 1.0%, cassia twig volatile oil: 1.2%, cocamide DEA: 1.0%, sodium laureth sulfate: 4%, TEA sodium lauryl sulfate: 3.0%, propylene glycol: 6% , Purified water: margin.

Example 20

Ethanol: 4%, Atractylodes volatile oil: 1.0%, Cassia twig volatile oil: 1.0%, Cocamide DEA: 0.8%, Sodium laureth sulfate: 3.5%, TEA sodium lauryl sulfate: 2.4%, Propylene glycol: 6% , Purified water: margin.

Example 21

Ethanol: 4%, Atractylodes japonicus volatile oil: 1.1%, Cassia twig volatile oil: 0.9%, Cocamide DEA: 0.6%, Sodium laureth sulfate: 3.8%, TEA sodium lauryl sulfate: 2.6%, Propylene glycol: 7% , Purified water: margin.

Example 22

Ethanol: 4%, Atractylodes volatile oil: 1.0%, Cassia twig volatile oil: 1.1%, Cocamide DEA: 0.8%, Sodium laureth sulfate: 3.5%, TEA sodium lauryl sulfate: 2.4%, Propylene glycol: 5% , Soda: 0.4%, baking soda 0.2%, purified water: balance.

Example 23

Ethanol: 4%, atractylodes volatile oil: 1.1%, cassia twig volatile oil: 0.8%, cocamide DEA: 0.6%, sodium laureth sulfate: 3.8%, sodium lauryl sulfate: 2.6%, propylene glycol: 5% , Soda: 0.5%, baking soda 0.5%, purified water: balance.

Example 24

Ethanol: 3%, atractylodes volatile oil: 1.0%, cassia twig volatile oil: 0.8%, medicinal koji oil 0.1%, cocamide DEA: 0.5%, sodium laureth sulfate: 3%, sodium lauryl sulfate: 2.0%, propylene glycol: 5%, purified water: the balance.

Example 25

Ethanol: 5%, atractylodes volatile oil: 1.5%, cassia twig volatile oil: 1.2%, medicinal koji oil 0.3%, cocamide DEA: 1.0%, sodium laureth sulfate: 4%, sodium lauryl sulfate: 3.0%, propylene glycol: 5%, purified water: the balance.

Example 26

Ethanol: 4%, atractylodes volatile oil: 1.5%, cassia twig volatile oil: 0.8%, medicinal koji oil 0.1%, cocamide DEA: 0.5%, sodium laureth sulfate: 3%, TEA sodium lauryl sulfate: 2.0%, propylene glycol: 6%, purified water: the balance.

Example 27

Ethanol: 4%, atractylodes volatile oil: 1.0%, cassia twig volatile oil: 1.2%, medicinal koji grass oil 0.2%, cocamide DEA: 1.0%, sodium laureth sulfate: 4%, TEA sodium lauryl sulfate: 3.0%, propylene glycol: 6%, purified water: the balance.

3.4 Application examples of compound essential oils in clothing disinfectant

A clothing disinfectant rich in pure natural plant essential oils, composed of the following raw materials by weight: Atractylodes volatile oil: 1.0%-1.5%, Cinnamon twig volatile oil: 8%-12%, Cocamide DEA: 0.5%-1.0%, Sodium lauryl ether sulfate: 3%-4%, TEA sodium lauryl sulfate: 2.0%-3.0%, glycerin: 5%-8%, purified water: the balance.

According to a preferred aspect of the present invention, there is also provided an enhanced clothing disinfectant, which is composed of the following components in weight percentage: Atractylodes volatile oil: 1.0%-1.5%, Guizhi volatile oil: 8%-12% , Herbal Aspergillus oil: 0.1-0.3%, cocamide DEA: 0.5%-1.0%, sodium laureth sulfate: 3%-4%, TEA sodium lauryl sulfate: 2.0%-3.0%, glycerin: 5%-8%, purified water: balance.

Preparation process: Mix purified water and glycerin evenly, add cocamide DEA, sodium lauryl ether sulfate, TEA sodium lauryl sulfate, soda, baking soda, stir to make it fully dissolved, add atractylodes oil and cinnamon sticks The oil and medicinal koji oil, and the specific added components were operated according to the following specific experimental examples. Stir mechanically for 20 minutes at room temperature, and the stirring speed is 200 rpm, so that it can be fully dissolved to a transparent and clear state to obtain a high-concentration laundry detergent. When using, take 60-80ml (about 3-4 bottle caps), mix with 10-50 times the amount of water according to the degree of sterilization, and stir well.

Example 28

Atractylodes japonicus volatile oil: 1.0%, cinnamon twig volatile oil: 8%, cocamide DEA: 0.5%, sodium laureth sulfate: 3%, TEA sodium lauryl sulfate: 2.0%, glycerin: 5%, purified water: remaining the amount.

Example 29

Atractylodes japonicus volatile oil: 1.5%, cassia twig volatile oil: 12%, cocamide DEA: 1.0%, sodium laureth sulfate: 4%, TEA sodium lauryl sulfate: 3.0%, glycerin: 8%, purified water: remaining the amount.

Example 30

Atractylodes japonicus volatile oil: 1.5%, cassia twig volatile oil: 8%, cocamide DEA: 0.5%, sodium lauryl ether sulfate: 3%, TEA sodium lauryl sulfate: 2.0%, glycerin: 5%, purified water: remaining the amount.

Example 31

Atractylodes volatile oil: 1.0%, Cassia twig volatile oil: 12%, cocamide DEA: 1.0%, sodium laureth sulfate: 4%, TEA sodium lauryl sulfate: 3.0%, glycerin: 8%, purified water: remaining the amount.

Example 32

Atractylodes japonicus volatile oil: 1.0%, cassia twig volatile oil: 10%, cocamide DEA: 0.8%, sodium laureth sulfate: 3.5%, TEA sodium lauryl sulfate: 2.4%, glycerin: 6%, purified water: remaining the amount.

Example 33

Atractylodes japonicus volatile oil: 1.1%, cassia twig volatile oil: 9%, cocamide DEA: 0.6%, sodium laureth sulfate: 3.8%, TEA sodium lauryl sulfate: 2.6%, glycerin: 7%, purified water: remaining the amount.

Example 34

Atractylodes volatile oil: 1.0%, Cassia twig volatile oil: 11%, cocamide DEA: 0.8%, sodium laureth sulfate: 3.5%, TEA sodium lauryl sulfate: 2.4%, glycerin: 6%, soda: 0.4% , Baking soda 0.2%, purified water: balance.

Example 35

Atractylodes volatile oil: 1.1%, Cassia twig volatile oil: 8%, cocamide DEA: 0.6%, sodium laureth sulfate: 3.8%, TEA sodium lauryl sulfate: 2.6%, glycerin: 7%, soda: 0.5% , Baking soda 0.5%, purified water: balance.

Example 36

Atractylodes volatile oil: 1.0%, cinnamon twig volatile oil: 8%, medicinal koji oil: 0.1%, cocamide DEA: 0.5%, sodium laureth sulfate: 3%, sodium lauryl sulfate: 2.0%, glycerin : 5%, purified water: balance.

Example 37

Atractylodes japonicus volatile oil: 1.5%, cinnamon twig volatile oil: 12%, medicinal koji oil: 0.2%, cocamide DEA: 1.0%, sodium laureth sulfate: 4%, sodium lauryl sulfate: 3.0%, glycerin : 8%, purified water: the balance.

Example 38

Atractylodes japonicus volatile oil: 1.5%, cinnamon twig volatile oil: 8%, medicinal koji oil: 0.3%, cocamide DEA: 0.5%, sodium laureth sulfate: 3%, sodium lauryl sulfate: 2.0%, glycerin : 5%, purified water: balance.

Example 39

Atractylodes japonicus volatile oil: 1.0%, cinnamon twig volatile oil: 12%, medicinal koji oil: 0.1%, cocoamide DEA: 1.0%, sodium laureth sulfate: 4%, sodium lauryl sulfate: 3.0%, glycerin : 8%, purified water: the balance.

Claims (7)

A pure natural compound essential oil with broad-spectrum bactericidal activity. The compound essential oil is composed of volatile oil extracted from the dried roots and stems of Atractylodes lanceolata or Atractylodes lanceolata and dried twigs or bark of Lauraceae cinnamon. The extracted volatile oil is obtained after mixing and compounding, wherein the volume content of the two volatile oils is: atractylodes volatile oil: 40-70 parts, and Guizhi volatile oil: 30-60 parts. The compound essential oil according to claim 1, which is prepared by steam distillation, specifically comprising the following steps: (1) Extraction of atractylodes oil: pulverize the rhizome of atractylodes japonicus into dry powder and put it into a steam distillation extractor containing 10 times the amount of water. The temperature is controlled at around 100℃. When the liquid flowing down the branch pipe of the extractor is colorless, Stop the extraction, use a separatory funnel to remove the distilled water from the bottom to obtain the oily crude extract, seal it and store it in a refrigerator at 4°C for later use; (2) Cinnamomum cassia oil extraction: pulverize cassia twig into dry powder, and put it into a steam distillation extractor containing 12 times the amount of water. The temperature is controlled at around 100℃. When the liquid flowing down the branch pipe of the extractor is colorless , Stop the extraction and remove the distilled water from the lower part with a separatory funnel to obtain the crude oily extract, which is sealed and stored in the refrigerator at 4℃ for later use; (3) Mixing atractylodes oil and cassia twig oil in a certain ratio to obtain a pure natural compound essential oil with broad-spectrum antibacterial activity. The compound essential oil according to claim 1, which is prepared by CO ₂ supercritical or subcritical fluid extraction method, specifically comprising the following steps: (1) Extraction of atractylodes rhizome: pulverize the rhizome of atractylodes japonicus into dry powder, put it in a CO ₂ supercritical fluid extraction tank of appropriate size, and directly extract with supercritical CO ₂ to obtain; (2) Cassia twig oil extraction: Cinnamon twig is crushed into dry powder, placed in a CO ₂ supercritical fluid extraction tank of appropriate size, and directly extracted with supercritical CO ₂ to obtain; (3) Mixing atractylodes oil and cassia twig oil in a certain ratio to obtain a pure natural compound essential oil with broad-spectrum antibacterial activity. The use of the compound essential oil according to claim 1 in medicine. The use of the compound essential oil according to claim 1 in disinfectant or sterilizing daily-use products. The use of the compound essential oil according to claim 1 in disinfection products for humans or animals. The use of the compound essential oil according to claim 1 in health products and foods.