WO2021017913A1 - Enhanced moisture-retaining cosmetic composition - Google Patents

Abstract

An enhanced moisture-retaining cosmetic composition, comprising (A) concentrated birch sap, the concentration factor being about 1.05-8 times, preferably 1.1-4 times, and most preferably 1.2-2 times; and (B) one or multiple substances selected from sodium hyaluronate, allantoin, ceramide, betaine and polyols.

Description

Enhanced moisturizing cosmetic composition Technical field

The present invention relates to an enhanced moisturizing cosmetic composition comprising (A) concentrated birch tree sap, the concentration of which is about 1.05-8 times, preferably about 1.1-4 times, more preferably about 1.2-2 times, (B ) One or more substances selected from sodium hyaluronate, allantoin, ceramide, betaine and polyol.

Background technique

The moisturizing ability of the skin has a certain correlation with the gloss, aging degree and the occurrence of skin diseases.

At present, the more recognized moisturizing methods are mainly divided into two aspects: one is to strengthen the control of moisture escape by enhancing the skin's epidermal barrier function and water retention ability; the other is to increase the expression of aquaporin in the dermis of the skin. The body's ability to supply water to epidermal cells. In addition, the proliferation and vitality of skin cells itself will affect the realization of the above functions.

At present, the most common moisturizing ingredient in the market is sodium hyaluronate, which can form a breathable and water-retaining film on the surface of the skin, capture water molecules in the air, and maintain the moisture content of the skin's surface. However, when the moisture content in the air is low, the single addition of sodium hyaluronate is difficult to meet consumer demand for moisturizing products. Therefore, the purpose of the present invention is to develop a moisturizing cosmetic composition with multiple moisturizing effects.

Birch is a deciduous tree of the birch family. There are currently about 100 species in the world, mainly distributed in the northern temperate zone and the cold temperate zone. Among them, there are about 29 varieties in my country, mainly distributed in the northeast, northwest, north and southwest. Birch trees are mostly grown in remote mountainous areas with little human intervention and no industrial pollution. Birch sap (also called birch sap) is the fresh sap from the birch bark being cut or the trunk is drilled. It is colorless or light yellow, free of precipitation and impurities, and has a light birch fragrance. The birch sap contains a lot of sugars, amino acids, vitamins, biotin, cytokinins, trace mineral elements, aromatic oils, betulin, saponin and other compounds. It has good moisturizing, anti-inflammatory, anti-wrinkle and whitening properties. And other skin care effects.

Summary of the invention

In one aspect, the present invention relates to a combination of (A) concentrated birch tree juice and (B) one or more selected from the group consisting of sodium hyaluronate, allantoin, ceramide, betaine, and polyols to enhance moisture retention For use in cosmetic compositions, the concentration factor of the concentrated birch tree juice is about 1.05-8 times, preferably about 1.1-4 times, more preferably about 1.2-2 times.

In another aspect, the present invention relates to an enhanced moisturizing cosmetic composition comprising (A) concentrated birch sap, the concentration of which is about 1.05-8 times, preferably about 1.1-4 times, more preferably about 1.2-2 Fold, and (B) one or more substances selected from sodium hyaluronate, allantoin, ceramide, betaine and polyol.

The enhanced moisturizing cosmetic composition of the present invention can promote keratin transglutaminase TGM1 and epidermal tight junction protein related to the epidermal barrier, filaggrin FLG related to skin water-holding capacity, and aquaporin AQP3 related to skin water absorption capacity The expression of related genes or proteins promotes the proliferation and differentiation of epidermal and dermal cells, reduces transdermal water loss, and has a more comprehensive skin moisturizing effect.

The moisturizing cosmetic composition does not contain any added water, but does not exclude the moisture inherently contained in each component.

In a preferred embodiment, chelating agents such as EDTA salt, sodium polyphosphate, sodium metaphosphate, and gluconic acid are not added to the moisturizing cosmetic composition.

The birch sap involved in the present invention is obtained from the genus Betula, Betula alba, Betula pubescens, Betula Pendula and Asian white birch (Betula platyphylla). Varieties. The birch sap is a colorless, transparent, no-sediment-free, and no-drug sap that is artificially collected by drilling holes at the base of the trunk of the birch from thawing to early spring. The birch sap is commercially available and used as it is, for example, purchased from Daxinganling Chaoyue Wild Berry Development Co., Ltd.

The concentrated birch sap of component (A) used in the present invention is obtained by concentrating the above-mentioned commercial products. Concentration methods are known in the art, such as heating concentration, low-temperature vacuum concentration, membrane concentration and the like. In the present invention, the concentration is preferably carried out by a low-temperature freeze concentration or membrane concentration process. For example, the commercially available birch juice stock solution is input into a low-temperature drying equipment, the temperature is lowered to -40°C to -70°C, and the vacuum is applied to 0.1-30Pa. Concentrate in vacuum at low temperature to obtain concentrated birch sap with a concentration ratio of 1.05-8 times.

The present inventors found that, compared with the unconcentrated birch sap stock, the concentrated birch sap with a concentration ratio of 1.05-8 times, preferably 1.1-4 times, especially 1.2-2 times, significantly increased the content of component (B) Moisturizing effect of cosmetic composition of sodium hyaluronate, allantoin, ceramide, betaine and/or polyol. This indicates that the combination of the concentrated birch tree juice and sodium hyaluronate, allantoin, ceramide, betaine and/or polyol produces a synergistic or synergistic effect.

Unexpectedly, the inventors also found that the promoting effect of concentrated birch sap on the moisturizing effect of component (B) and the degree of concentration is not a simple linear relationship, but as the concentration ratio increases, it first increases and then decreases. trend. Therefore, controlling the concentration ratio of birch sap is key. In the present invention, controlling the concentration ratio of birch sap is about 1.05-8 times, preferably about 1.1-4 times, more preferably about 1.2-2 times.

The content of the component (A) concentrated birch sap is about 18-98% by weight, preferably 20-95% by weight, more preferably 22-92% by weight, most preferably 30-90% by weight, based on the moisturizing cosmetic composition The total weight of the object.

Examples of the sodium hyaluronate in the component (B) include, but are not limited to, large molecular weight sodium hyaluronate (1800-2200KDa), medium molecular weight sodium hyaluronate (1000-1800KDa), small molecular weight sodium hyaluronate ( One or more of 10-1000KDa), hydrolyzed sodium hyaluronate (1-10KDa), acetylated sodium hyaluronate, etc. Sodium hyaluronate can be prepared by methods known in the art, for example, firstly, sodium hyaluronate is prepared from a plant-derived medium (peptone, glucose, etc.) by fermentation. Sodium hyaluronate is also commercially available, for example, from Huaxi Freda Biomedical Co., Ltd.

Examples of polyols in the component (B) include, but are not limited to, glycerin, diglycerin, butylene glycol, 1,2-propanediol, 1,3-propanediol, dipropylene glycol, 1,2-pentanediol One or more of, polyethylene glycol-8, polyethylene glycol-32, xylitol, sorbitol, erythritol, etc. The materials are all commercially available.

Allantoin, ceramide and betaine in the component (B) are also known in the art, and they are all commercially available.

The content of the component (B) is about 0.005-10% by weight, preferably 0.01-8% by weight, more preferably 0.1-6% by weight, most preferably about 0.5-5% by weight, based on the total moisturizing cosmetic composition weight.

In addition to the components (A) and (B), the moisturizing cosmetic composition may optionally include components (C) commonly used in moisturizing cosmetics, examples of which include, but are not limited to, vehicles, active ingredients, Surfactants and auxiliary materials, etc. These ingredients are known in the art, and those skilled in the art can choose the type and amount of component (C) according to their needs. For example, the content of component (C) is usually 0-80%, based on the moisturizing cosmetic combination The total weight of the object.

The vehicle is those known in the art, such as diluents, dispersants and carriers, and examples thereof include, but are not limited to, ethanol, dipropylene glycol, butylene glycol, and the like. The content of the vehicle in the cosmetic composition is known in the art, for example, it usually accounts for 0.5-20% of the total weight of component (C).

The active ingredients are those known in the art, examples of which include, but are not limited to, emollients, other moisturizers, and the like.

Examples of the emollient include, but are not limited to, olive oil, macadamia nut oil, sweet almond oil, grape seed oil, avocado oil, corn oil, sesame oil, soybean oil, peanut oil, white flower seed oil, safflower seed oil, Dogtooth rose hip oil, argan tree kernel oil, jojoba seed oil, sunflower seed oil, palm tree fruit oil, squalane, ethylhexyl palmitate, isopropyl myristate, hydrogenated polyisobutylene, isotene Hexane, isododecane, diethylhexyl carbonate, dioctyl carbonate, isopropyl lauroyl sarcosine, isononyl isononanoate, hydrogenated polydecene, glycerol tri(ethylhexanoate) , Cetyl alcohol ethyl hexanoate, bis-diethoxy diethylene glycol cyclohexane 1,4-dicarboxylate, caprylic/capric triglyceride, oleyl erucate, octyldodecanol Myristate, Octyldodecanol, Dimethicone, Octyl Dimethicone, Cetyl Dimethicone, Cyclopenta Dimethicone, etc. One or more. Examples of solid emollients include, but are not limited to, cetyl alcohol, stearyl alcohol, cetearyl alcohol, behenyl alcohol, scylitol, lauric acid, myristic acid, palmitic acid, stearic acid, beeswax, candelilla Tree wax, carnauba wax, lanolin, ozokerite, jojoba seed wax, paraffin wax, microcrystalline wax, hydrogenated rice bran wax, hydrogenated coconut oil glycerides, glyceryl behenate/eicosanate, myristyl alcohol One or more of myristate, bis-diglyceride polyacyl adipate-2, shea butter, and muluxing palm seed butter. The content of the emollient in the cosmetic composition is known in the art, for example, it usually accounts for 1-50% of the total weight of component (C).

Examples of the other humectants include, but are not limited to, methylglucitol-10, methylglucitol-20, PEG/PPG-17/6 copolymer, glycerol-7, glycerol polyether -26, glycerol glucoside, PPG-10 methyl glucose ether, PPG-20 methyl glucose ether, PEG/PPG/polybutylene glycol-8/5/3 glycerol, sucrose, trehalose, rhamnose, One or more of mannose, raffinose, glycerol polyether-5 lactate, sodium polyglutamate, hydrolyzed sclerotium, sprouting enzyme polysaccharide, tremella polysaccharide, caper seed polysaccharide, etc. The content of the moisturizer in the cosmetic composition is known in the art, for example, it usually accounts for 1-30% of the total weight of the component (C).

Examples of the auxiliary materials include, but are not limited to, emulsifiers, thickeners, preservatives, perfumes and the like.

Examples of the emulsifier include, but are not limited to, cetearyl oleate, sorbitan oleate, polysorbate-60, polysorbate-80, methylglucose sesquistearic acid Ester, PEG-20 methyl glucose sesquistearate, PEG-40 hydrogenated castor oil, PPG-26-butanol-26, PEG-4 polyglycerol-2 stearate, PEG-60 hydrogenated Castor oil, steareth-2, steareth-21, PPG-13-decyltetradeceth-24, cetearyl glucoside, PEG-100 stearate, glycerin Stearate, Glyceryl Stearate SE, Coco Glucoside, Ceteareth-25, PEG-40 Stearate, Polyglyceryl-3 Methyl Glucose Distearate, Glyceryl stearate citrate, polyglyceryl-10 stearate, polyglyceryl-10 myristate, polyglyceryl-10 dioleate, polyglyceryl-10 laurate, polyglyceryl-10 isostearate Fatty acid ester, polyglyceryl-10 oleate, polyglyceryl-10 diisostearate, polyglyceryl-6 laurate, polyglyceryl-6 myristate, sucrose stearate, sucrose polystearate One or more of acid esters. The content of the emulsifier in the cosmetic composition is known in the art, for example, it usually accounts for 0.5-10% of the total weight of component (C).

Examples of the thickener include, but are not limited to, carbomers, acrylic acid (ester) and its derivatives, xanthan gum, gum arabic, polyethylene glycol-14M, polyethylene glycol-90M, succinyl poly One or more of high molecular polymers such as sugar, hydroxyethyl cellulose, hydroxypropyl cellulose, and hydroxypropyl methyl cellulose. The content of the thickener in the cosmetic composition is known in the art, for example, it usually accounts for 0.1-10% of the total weight of the component (C).

Examples of the preservatives include, but are not limited to, methylparaben, propylparaben, phenoxyethanol, benzyl alcohol, phenethyl alcohol, bis(hydroxymethyl)imidazolidinylurea, potassium sorbate, sodium benzoate, chlorobenzene Glycerol, sodium dehydroacetate, caprylic hydroxamic acid, 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, caprylyl glycol, glyceryl caprylate, undecylenic acid One or more of glycerides, sorbitan caprylate, ethylhexylglycerol, tree peony root extract, etc. The content of the preservative in the cosmetic composition is known in the art, for example, it usually accounts for 0.01-2% of the total weight of the component (B).

The moisturizing cosmetic composition of the present invention can be prepared by any suitable method known in the art. For example, it can be prepared by dissolving tanks, emulsifying pots, dispersers, and delivery pumps commonly used in the cosmetics field. When preparing, put the water-soluble substance into the water-phase dissolving kettle, and the oil-soluble substance into the oil-phase dissolving kettle, and heat the temperature of the two kettles to about 80°C. For the raw materials that are easy to agglomerate, they can be mixed with a disperser first. Pre-dispersion. After the dissolution is completed, the oil phase and the water phase are transported to the emulsifying pot for homogenization and emulsification for about 5-15 minutes. After the emulsification is completed, the temperature of the material body is reduced to normal temperature, optional flavors, preservatives, etc. are added, and the pH of the product is adjusted as necessary. The products can be filled and shipped only after the relevant test indicators are qualified.

The above preparation methods can be deleted or adjusted according to dosage form requirements. According to needs, the moisturizing cosmetic composition can be made into various dosage forms such as creams, creams, lotions, and essences.

Example

Hereinafter, the present invention will be further described in detail with reference to the embodiments. However, it should be understood that these embodiments and comparative examples are only used to specifically illustrate the present invention, and should not be construed as limiting the scope of the appended claims of the present invention in any form.

Example 1 : Preparation of compositions A, A1, B, B1, B2, B3, B4, C, C1, C2, D, D1, D2, E, E1, E2, F, F1, F2 and J

The specific formula of each composition is as follows:

Note: The data in the table represents the weight% of each component, based on the total weight of the composition, the same below.

The above compositions are prepared as follows:

1. Input the fresh white birch sap stock solution purchased from Daxinganling Chaoyue Wild Berry Development Co., Ltd. into low-temperature drying equipment, cool to -65℃, vacuum to 0.1Pa, and concentrate to 1.2 times, 4 times, and 9 times;

2. Mix raw materials 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 to obtain the corresponding composition.

Example 2 : Composition A, A1, B, B1, B2, B3, B4, C, C1, C2, D, D1, D2, E, E1, E2, F, F1, F2 versus keratin transglutaminase TGM1 , Epidermal tight junction protein (ZO-1 and CLDN1), filaggrin FLG and aquaporin AQP3 gene expression

Experimental instruments: fluorescence quantitative PCR instrument (Roche), ultra-clean workbench (Su Jing), carbon dioxide incubator (Binder), microplate reader (BIO-TEK), micro oscillator.

Experimental reagents and consumables: human primary keratinocytes, 6-well plates, keratinocyte culture medium, RNA extraction kit, reverse transcription kit, Trizol lysis solution, etc.

The steps of gene expression analysis based on fibroblasts are as follows:

(1) Inoculation: Inoculate cells into a 6-well plate at a seeding density of 5E5/well, and incubate overnight in a 37°C and 5% CO ₂ incubator;

(2) Administration: When the plating rate of cells in the 6-well plate reaches about 60%, add the test substance of each group, and each group has 6 multiple wells;

(3) Sample collection: After 24h in a 37°C and 5% CO ₂ incubator, discard the culture solution, add 1mL Trizol to each well, pipette to lyse the cells, and collect the sample;

(4) PCR detection: RNA is extracted, reverse transcribed to cDNA, and then subjected to fluorescence quantitative PCR detection;

(5) Analysis: The 2- ^△△CT method is used for calculation of the results, and the T-Test method is used for statistical analysis.

The test results are shown in the table below.

sample TGM1 ZO-1 CLDN1 FLG AQP3 A 4.52±0.45 2.57±0.38 6.26±0.72 3.45±0.38 3.99±0.47 A1 14.94±1.39 8.19±0.92 17.61±2.84 9.28±1.44 9.85±0.86 B 4.56±0.63 2.53±0.19 6.81±0.64 3.75±0.22 4.08±0.49 B1 11.43±0.68 8.13±0.49 18.86±1.94 10.52±0.96 11.75±0.63 B2 28.81±2.89 19.25±1.73 32.34±3.32 22.29±1.87 22.56±2.47 B3 21.54±2.16 13.77±1.28 24.17±2.09 15.15±1.21 16.48±1.44 B4 11.62±1.28 7.99±0.67 18.04±1.72 10.03±0.96 10.89±1.01 C 3.82±0.27 3.18±0.29 6.07±0.65 3.83±0.31 3.84±0.43 C1 11.25±1.11 8.38±0.64 18.02±1.71 10.89±1.03 11.14±1.15 C2 27.25±2.31 20.04±2.36 33.29±3.57 21.56±1.98 20.47±2.16 D 4.91±0.47 2.88±0.23 6.59±0.70 3.37±0.35 4.12±0.39

D1 12.71±1.48 8.93±5.79 19.18±2.07 9.96±0.93 12.04±1.26 D2 29.27±3.04 18.72±1.56 33.67±3.44 21.38±2.24 22.86±1.95 E 5.04±0.48 3.85±0.33 7.15±0.69 3.78±0.33 4.22±0.40 E1 12.56±1.32 9.73±1.06 18.66±1.54 8.91±0.85 10.28±0.97 E2 29.46±2.88 21.07±2.67 34.18±3.55 21.85±2.06 21.79±2.11 F 4.68±0.39 3.01±0.28 6.54±0.47 3.65±0.38 4.11±0.36 F1 11.85±0.96 8.43±0.72 18.54±1.67 11.06±1.08 11.87±1.14 F2 28.63±2.69 19.47±1.83 32.77±3.15 22.70±2.12 22.36±2.18 J 1.21±0.09 1.16±0.11 1.89±0.26 1.25±0.12 1.04±0.09

The above results show that compared with the blank control J, other compositions can improve the skin barrier-related cutin transglutaminase (TGM1), epidermal tight junction protein Z0-1 and CLDN in keratinocytes, and the skin The gene expression levels of filaggrin (FLG) related to water retention capacity and aquaporin (AQP3) related to skin water absorption capacity, indicating that it contains birch sap, concentrated birch sap, hydrolyzed sodium hyaluronate, glycerin, nerves The composition of amide, allantoin or betaine all has a certain moisturizing effect. However, comparing the results of composition B2, C2, D2, E2, F2 and composition A1, B, C, D, E, and F respectively, it can be clearly seen that the 1.2 times concentrated birch tree juice and hydrolyzed sodium hyaluronate, The combination of glycerin, ceramide, allantoin or betaine significantly increased the gene expression level of each protein, which fully demonstrated that birch tree juice and other moisturizing ingredients had a synergistic effect; in particular, the comparison composition B2 , B3, B4, C2, D2, E2, F2 and the results of the composition B1, C1, D1, E1, F1, it can be clearly seen that, compared with the non-concentrated birch sap stock, the concentrated birch sap significantly increases the content The moisturizing effect of the cosmetic composition of hydrolyzed sodium hyaluronate, glycerin, ceramide, allantoin or betaine, and the concentration factor of the concentrated birch sap is about 1.05-8 times, preferably about 1.1-4 times.

Example 3 : The effect of compositions B1, B2 and C1, C2 on the protein expression of cutin transglutaminase TGM1, epidermal tight junction proteins (ZO-1 and CLDN1), filaggrin FLG and aquaporin AQP3

In this example, the effects of the above-mentioned compositions B1, B2 and C1, C2 on the expression of aging-related proteins were further tested and compared.

Experimental equipment: ultra-clean workbench (Su Jing), plate washer (BIO-RAD), microplate reader (BIO-TEK), carbon dioxide incubator (Binder)

Experimental reagents and consumables: human primary keratinocytes, 12-well plates, keratinocyte culture medium, ELISA test kits with different indicators, etc.

The test steps are as follows:

(1) Inoculation: Inoculate in a 12-well culture plate at an inoculation density of 2E5/well, cultivate in an incubator at 37°C and 5% CO2, and change the medium every two days;

(2) Dosing: When the cell fusion reaches more than 60% again, add different groups of test substances, each group has 6 multiple holes;

(3) Receiving samples: After 48 hours in a 37°C and 5% CO ₂ incubator, discard the culture solution, add 1 mL Trizol to each well, pipette to lyse the cells, and collect the samples;

(4) Detection: Perform index determination according to the determination method of ELISA kit;

(5) Analysis: T-Test method is used for statistical analysis.

The test results are shown in the table below.

sample TGM1 ZO-1 CLDN1 FLG AQP3 B1 3.85±0.34 3.71±0.32 4.06±0.33 3.89±0.29 3.23±0.28 B2 6.74±0.55 6.07±0.52 7.98±0.81 7.74±0.63 6.05±0.60 C1 4.17±0.65 4.07±0.38 4.58±0.42 3.75±0.38 3.12±0.29 C2 7.03±0.71 6.57±0.59 7.75±0.73 7.93±0.71 6.62±0.67

The above results indicate that the compositions B2 and C2 containing 1.2 times concentrated birch sap and hydrolyzed sodium hyaluronate or glycerin are significantly higher than the compositions B1 and C1 containing birch sap stock and hydrolyzed hyaluronic acid or glycerin. The gene expression level of each protein was determined, which showed that the synergistic effect of concentrated birch sap on hydrolyzed sodium hyaluronate or glycerin was significantly higher than that of birch sap stock, which was unexpected.

Example 4 : Preparation of compositions G, G1, G2 and J1

G: Hydrolyzed sodium hyaluronate

G1: Birch sap stock liquid + hydrolyzed sodium hyaluronate

G2: 1.2 times concentrated birch sap + hydrolyzed sodium hyaluronate

J1: Blank control

To ingredient To G G1 G2 J1 1 Birch sap To —— 92.3 —— —— 2 1.2 times concentrated birch sap To —— —— 92.3 —— 3 Hydrolyzed Sodium Hyaluronate To 0.5 0.5 0.5 —— 4 Allantoin To —— —— —— —— 5 water To 92.3 —— —— 92.8 6 Methyl paraben To 0.15 0.15 0.15 0.15 7 Xanthan gum To 0.1 0.1 0.1 0.1 8 glycerin To 3.5 3.5 3.5 3.5 9 Dipropylene glycol To 3 3 3 3 10 Arginine To 0.1 0.1 0.1 0.1 11 Phenoxyethanol To 0.3 0.3 0.3 0.3 12 Octyl glycol To 0.05 0.05 0.05 0.05

The above compositions are prepared as follows:

1. Put the raw materials 1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12 into the emulsification pot, stir and heat up to 80°C, keep it for 10 minutes.

2. Cool down to 45°C while stirring.

3. Add raw material 10 and stir for 10 minutes.

4. Discharge after passing the inspection.

Example 5 : Influence of composition G, G1, G2 and J1 on skin moisturizing effect

Use Corneometer (Courage & Khazaka, Germany) skin surface water content measuring instrument. Approximately 0.02 grams of each composition sample was dropped into the pre-marked square area on the volunteer's arm once a day in the morning and evening, and the sample was evenly smeared on the area. The moisture content (unit: a.u.) of the skin epidermis before use (reference) and 14 days of use was measured with an instrument, and the test was repeated three times for each area and the average value was taken. The test results are shown in the table below.

G 122%±4% G1 136％±5%* G2 158%±10%** J1 110%±3%

Note: * means significant compared with composition G (hydrolyzed sodium hyaluronate group), and the P value is less than 0.05;

** indicates that it is extremely significant compared with composition G (hydrolyzed sodium hyaluronate group), and the P value is less than 0.01.

The above results indicate that after 14 days of continuous use, compared with the blank control group J1, the compositions G, G1, and G2 can significantly increase the skin epidermal moisture content of the subjects. Compared with composition G containing only hydrolyzed sodium hyaluronate, compositions G1 and G2 containing both hydrolyzed sodium hyaluronate and birch sap or concentrated birch sap can significantly improve the moisturizing properties of the skin. In particular, the composition G2 can significantly increase the moisture content of the skin epidermis of the subject.

Example 6 : Moisturizing facial mask

The formula of the moisturizing facial mask is as follows:

Serial number ingredient weight% 1 1.5 times concentrated birch sap 90.75 2 Hydrolyzed Sodium Hyaluronate 0.05 3 Sodium polyglutamate 0.05 4 Sodium hyaluronate 0.05 5 Allantoin 0.1 6 Xanthan gum 0.1 7 Methyl paraben 0.2 8 Panthenol 0.3 9 Betaine 2 10 glycerin 2 11 Dipropylene glycol 4 12 Carbomer 0.2 13 Octyl glycol 0.1 14 Tromethamine 0.1

The moisturizing facial mask composition is prepared as follows:

1. Disperse the raw material 6 evenly with the raw material 10.

2. Put the raw material 1 into the emulsifying pot, sprinkle into the raw material 12 while stirring, and then add the raw materials 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 after the raw material 12 is completely swelled.

3. Heat up to 80°C while stirring, homogenize at high speed for 5 minutes, and keep warm for 10 minutes.

4. Cool down to 40°C while stirring, and add raw materials 13, 14.

5. Stir for 10 minutes.

6. Discharge after passing the inspection.

After 20 subjects used the moisturizing mask of the above formula for 2 weeks, subjective evaluation of the use was made. The results showed that 20 subjects all reported that the dryness of the skin was significantly improved and the water content increased significantly after use. Among them, 18 people reported that their skin became smoother and more tender after use, and continued use can significantly improve the skin barrier and reduce skin problems.

Example 7 : Moisturizing lotion

The formula of the moisturizing lotion is as follows:

Serial number ingredient weight% 1 4 times concentrated birch sap 84 2 Hydrolyzed Sodium Hyaluronate 0.05 3 Sodium hyaluronate 0.05 4 Allantoin 0.1 5 Xanthan gum 0.1 6 Methyl paraben 0.2 7 Polysorbate-60 0.3 8 PEG-60 hydrogenated castor oil 0.5 9 Betaine 1 10 Dipropylene glycol 4 11 glycerin 5 12 Carbomer 0.2 13 Phytosterol/octyldodecanol lauroyl glutamate 1 14 Squalane 2 15 Tromethamine 0.1 16 PEG/PPG-17/6 copolymer 1 17 Phenoxyethanol 0.4

The above moisturizing lotion composition is prepared as follows:

1. Disperse the raw material 5 evenly with the raw material 10.

2. Put the raw material 1 into the emulsifying pot, sprinkle into the raw material 12 while stirring, and then add the raw materials 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 after the raw material 12 is completely swelled.

3. Heat up to 80°C while stirring, homogenize at high speed for 5 minutes, and keep warm for 10 minutes.

4. Add raw material 13 and raw material 14, homogenize at high speed for 5 minutes and keep warm for 10 minutes.

5. Cool down to 60°C while stirring, and add raw material 15.

6. Cool down to 50°C, add raw material 16 and raw material 17, and homogenize slowly for 3 minutes.

7. Cool down to 40°C while stirring.

8. Discharge after passing the inspection.

Example 8: Moisturizing cream

The formula of the moisturizing cream is as follows:

Serial number ingredient weight% 1 2 times concentrated birch sap 62.2 2 Sodium polyglutamate 0.1 3 Hydrolyzed Sodium Hyaluronate 0.1 4 Xanthan gum 0.2 5 Allantoin 0.2 6 Methyl paraben 0.2 7 Hydrogenated Lecithin 0.5 8 Panthenol 0.5 9 Betaine 3 10 Butanediol 4 11 glycerin 6 12 Carbomer 0.2 13 Propylparaben 0.1 14 Phytosterol/octyldodecanol lauroyl glutamate 1 15 Microcrystalline wax 1 16 C10-18 fatty acid triglycerides 2 17 Cetearyl glucoside, cetearyl alcohol 2 18 Cetearyl alcohol 2 19 PEG-100 stearate, glyceryl stearate 3 20 Caprylic/Capric Triglyceride 4 twenty one Coco-Caprylate/Caprate 5 twenty two Polydimethylsiloxane 2 twenty three Tromethamine 0.2 twenty four Phenoxyethanol 0.5

The above moisturizing cream composition is prepared as follows:

1. Disperse the raw material 4 with the raw material 11 uniformly.

2. The raw material 7 is heated and dissolved with the raw material 10.

3. Put the raw material 1 into the water-phase pot, stir in the raw material 12 while stirring, add the raw materials 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 after the raw material 12 is completely swelled, and heat up to 80 ℃.

4. Put the raw materials 13, 14, 15, 16, 17, 18, 19, 20, 21, 22 into the oil phase pot and raise the temperature to 80°C.

5. Pump the raw materials in the water phase pot into the emulsifying pot and homogenize at high speed for 5 minutes.

6. Pump the raw materials in the oil phase pot into the emulsifying pot, homogenize at high speed for 5 minutes, and keep warm for 10 minutes.

7. While stirring, lower the temperature to 50°C, put in raw material 23 and raw material 24, and homogenize slowly for 3 minutes.

8. Cool down to 40°C while stirring.

9. Discharge after passing the inspection.

In this example, a Corneometer (Courage & Khazaka, Germany) was used to test the skin moisture content of 20 volunteers. The test method is as follows:

Approximately 0.02 grams of each composition sample was dropped into the pre-marked square area on the volunteer's arm once a day in the morning and evening, and the sample was evenly smeared on the area. The moisture content (unit: a.u.) of the skin epidermis before use (reference) and 14 days of use was measured with an instrument, and the test was repeated three times for each area and the average value was taken.

The test results showed that the average moisture content of the skin of 20 subjects increased by 158%, indicating that the cream has extremely significant moisturizing properties.

Example 9 : Moisturizing facial cleanser

The formula of the moisturizing cleanser is as follows:

Serial number ingredient weight% 1 3 times concentrated birch sap 40.59 2 Hydrolyzed Sodium Hyaluronate 0.01 3 Polyethylene glycol-14M 0.1 4 Acrylic (ester)/C10-30 alkanol acrylate cross-linked polymer 0.5 5 Sodium Methyl Cocoyl Taurate 5 6 Sodium Lauroyl Glutamate twenty two 7 glycerin 25 8 Stearic acid 0.5 9 Lauric acid 3.5 10 PEG-120 Methyl Glucose Trioleate, 1,3-Propanediol 1 11 Citric acid 0.3 12 Sodium dihydrogen phosphate 1 13 Phenoxyethanol 0.5

The moisturizing cleanser composition is prepared as follows:

1. Raw material 2 and raw material 3 are evenly dispersed with raw material 7.

2. Put the raw material 1 into the emulsifying pot, sprinkle raw material 4 while stirring, add raw material 2, raw material 3 and raw material 7 after raw material 4 is completely swelled, heat up to 80°C while stirring, and homogenize at high speed for 5 minutes.

3. Put in raw material 5 and raw material 6, keep warm and stir until the material becomes clear and transparent.

4. Put in raw material 8 and raw material 9, keep warm and stir for 20 minutes.

5. Put in raw materials 10, 11, 12 and raw material 13, and keep it warm for 10 minutes.

6. Cool down to 40°C while stirring.

7. Discharge after passing the inspection.

Twenty subjects used the moisturizing cleanser of the above formula for 2 weeks and subjectively evaluated the usage. As a result, 19 people reported that the skin did not feel tight or dry after washing.

Example 10 : Moisturizing essence

The formula of the moisturizing essence is as follows:

The above moisturizing essence composition is prepared as follows:

1. Disperse the raw material 3 uniformly with the raw material 11.

2. Put the raw material 1 into the emulsifying pot, and sprinkle raw materials 14 and 15 into the emulsifying pot while stirring. After raw materials 14 and 15 are completely swelled, add raw materials 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 and raw material 13, heat up to 80°C while stirring, homogenize at high speed for 5 minutes, and keep warm for 10 minutes.

3. Cool down to 50°C while stirring, add raw materials 16, 17 and 18, and homogenize at high speed for 5 minutes.

4. Cool down to 50°C while stirring, and add raw material 19 and raw material 20.

5. Cool down to 40°C while stirring.

6. Discharge after passing the inspection.

After using the moisturizing essence of the above formula for 2 weeks, 20 subjects subjectively evaluated the usage. As a result, 19 people reported that the dryness of the skin was significantly improved and the water content increased significantly. Among them, 17 reported that their skin has become smoother, softer and more elastic, and dry lines on the face and eyes have been significantly improved.

The technical solutions of the above-mentioned embodiments are preferred implementations of the present invention. Several improvements and changes can be made without departing from the principle of the present invention, and these improvements and changes should also be regarded as falling within the protection scope of the present invention.

Claims (6)

(A) Use of a combination of concentrated birch tree juice and (B) one or more selected from sodium hyaluronate, allantoin, ceramide, betaine and polyol in a moisturizing cosmetic composition, wherein The concentration factor of the concentrated birch sap is 1.05-8 times, preferably 1.1-4 times, more preferably 1.2-2 times. The use of claim 1, wherein the moisturizing cosmetic composition does not contain any added water. A moisturizing cosmetic composition comprising (A) concentrated birch sap, the concentration of which is 1.05-8 times, preferably 1.1-4 times, more preferably 1.2-2 times, and (B) is selected from sodium hyaluronate , Allantoin, ceramide, betaine and one or more of polyols. The moisturizing cosmetic composition of claim 3, wherein the content of the component (A) concentrated birch sap is 18-98% by weight, preferably 20-95% by weight, more preferably 22-92% by weight, most preferably 30-90 % By weight, based on the total weight of the moisturizing cosmetic composition. The moisturizing cosmetic composition of claim 3 or 4, wherein the content of the component (B) is 0.005-10% by weight, preferably 0.01-8% by weight, more preferably 0.1-6% by weight, most preferably about 0.5-5% by weight Weight, based on the total weight of the moisturizing cosmetic composition. The moisturizing cosmetic composition of any one of claims 3-5, wherein the moisturizing cosmetic composition does not contain any added water.