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WO2021015648A2 - Additif alimentaire protéiné pour animaux de ferme et poissons d'élevage - Google Patents

Additif alimentaire protéiné pour animaux de ferme et poissons d'élevage Download PDF

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Publication number
WO2021015648A2
WO2021015648A2 PCT/RU2020/050121 RU2020050121W WO2021015648A2 WO 2021015648 A2 WO2021015648 A2 WO 2021015648A2 RU 2020050121 W RU2020050121 W RU 2020050121W WO 2021015648 A2 WO2021015648 A2 WO 2021015648A2
Authority
WO
WIPO (PCT)
Prior art keywords
strain
fish
protein
additive
feed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/RU2020/050121
Other languages
English (en)
Russian (ru)
Other versions
WO2021015648A3 (fr
Inventor
Елена Сергеевна БАБУСЕНКО
Валерий Алексеевич Быков
Маргарита Витальевна ЛАЛОВА
Леонид Евгеньевич Левитин
Александр Иванович Сафонов
Наталья Леонидовна Куликова
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Obshchestvo S Ogranichennoy Otvetstvennostyu "giprobiosintez"
GiproBioSintez OOO
Original Assignee
Obshchestvo S Ogranichennoy Otvetstvennostyu "giprobiosintez"
GiproBioSintez OOO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Obshchestvo S Ogranichennoy Otvetstvennostyu "giprobiosintez", GiproBioSintez OOO filed Critical Obshchestvo S Ogranichennoy Otvetstvennostyu "giprobiosintez"
Priority to JP2021575092A priority Critical patent/JP7618246B2/ja
Priority to EA202091614A priority patent/EA202091614A1/ru
Publication of WO2021015648A2 publication Critical patent/WO2021015648A2/fr
Publication of WO2021015648A3 publication Critical patent/WO2021015648A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Definitions

  • the invention is intended for use in animal husbandry and fish farming, in particular, it relates to feed bioadditives for feed when growing farm animals and fish in artificial reservoirs to increase productivity, natural resistance.
  • the introduction of a protein feed additive into the mixture allows to optimize the nutritional composition of feed, improve their bioavailability and influence on the rate of development of animals and fish, provide a prophylactic effect, etc.
  • the disadvantage of the known strains of microorganisms is the lack of the ability to actively grow under conditions of a long non-sterile cultivation process.
  • the use of the yeast of the genus Candida as a producer culture requires the complication of the production technology by the mandatory introduction of the stage of heat treatment of the resulting feed biomass to ensure the absence of living cells in the finished product.
  • methanotrophic bacteria that produce protein.
  • methanotrophic bacteria actively process natural gas, multiply rapidly and build up their biomass rich in valuable protein, vitamins and other biologically active substances.
  • methane for protein production has a number of advantages over liquid hydrocarbons: large reserves of natural gas, good transportability, and the possibility of obtaining a finished product without additional purification from the substrate.
  • Russia has large natural gas reserves, according to some sources, they account for up to 40% of the world.
  • the introduction of microbiological protein production at Russian enterprises promises not only an economic effect, but is also capable of ensuring the country's food security.
  • strains of bacteria that produce fodder protein belonging to different types of methane-oxidizing bacteria (methanotrophs), for example, for example, strains of the genus Pseudomonas methanica, Methylococcus capsulatus BKM B-2116, Methylocystis parvus BKM B-2129, MethylosMinpo sporium BKM BKM B-2129, MethylosMinpox sporium BKM BKM B-2117, Methylobacter acidophilus, Methylomonas rubra VSB-90, Methylococcus sp. ChM-9, Methylococcus capsulatus VSB-874, Methylococcus minimus, Methylomonas methanica, Methylomonas agile.
  • methane-oxidizing bacteria methane-oxidizing bacteria
  • RF2687137 which relates to biotechnology and can be used to obtain a microbial protein mass for animal feeding.
  • Known invention which relates to biotechnology and can be used to obtain a microbial protein mass for animal feeding.
  • the strain of heterotrophic bacteria Stenotrophomonas acidaminiphila GBS-15-2 has the ability to use the products of co-oxidation of methane homologues present in natural gas.
  • the strain of heterotrophic bacteria Stenotrophomonas acidaminiphila was deposited in the All-Russian collection of microorganisms at the Institute of Biochemistry and Physiology of Microorganisms of G.K. Skryabin RAS under the registration number VKM B-3264D.
  • the strain of heterotrophic bacteria Stenotrophomonas acidaminiphila VKM B-3264D can be used to obtain microbial protein mass (RF JV "2687136).
  • the closest of the analogs in technical essence and the claimed effect is a strain of methane-oxidizing bacteria Methylococcus capsulatus GBS-15.
  • the area of application of the strain is the production of protein-vitamin complexes for balancing feed for agricultural animals. Deposited in the All-Russian Collection of Industrial Microorganisms under the number VKPM B -12549.
  • the strain provides biomass accumulation up to 32 g / l. (RF N ° 2613365).
  • the content of crude protein in biomass is not less than 79%.
  • the technical task is to expand the range of used protein feed additives based on methane-oxidizing bacteria Methylococcus capsulatus for farm animals and fish grown in artificial reservoirs, which have a higher technological potential and cost-effective industrial process, high protein content.
  • the technical result obtained from the use of the proposed feed additive is the creation of an effective technology for obtaining the additive, improved operational properties of the additive by increasing the productivity of farm animals and fish.
  • the technical result was achieved through the use of a protein feed additive for farm animals and cultured fish, including a strain of methane-oxidizing bacteria Methylococcus capsulatus (GBS-15) VKPM B-12549.
  • the supplement additionally contains a strain of heterotrophic bacteria Cupriavidus gilardii (GBS-15-1) VKM B-3265D and / or a strain of heterotrophic bacteria Stenotropkomonas aeidanmupkila (GBS-15-2) VKM B-3264D and / or a strain of heterotrophic bacteria Klebsiella pneumonia " GKPM - OOBOLENSK "V-8465, taken in equal amounts, with the following ratio of components, wt.%:
  • Feeding the diet with a protein feed supplement containing the proposed mixture of strains contributes to an increase in body weight due to muscle tissue gain. Meat quality is improved by reducing muscle fat, reducing subcutaneous fat, and increasing the muscle eye area.
  • the invention will increase the productivity of animals and fish, their survival, the ecological safety of meat, and obtain meat with functional properties.
  • the claimed ratio of the components of the protein feed additive provides the creation of an easily digestible high-protein additive with a content of heterotrophic bacteria.
  • a protein feed additive for farm animals and fish is obtained by mixing the components according to the stated ratio and then, if necessary, granulated or extruded.
  • Table 1 shows the formulations of the proposed protein supplements.
  • option 4 an additive based on one GBS-15 strain.
  • the use of the proposed protein feed additive is essential in mixtures of feed additives to optimize the nutritional composition of feed, improve their bioavailability.
  • the need for fish, animals and poultry for the necessary nutritional components is ensured, and feed costs per unit of production are reduced.
  • Methanotroph Methylococcus capsulatus GBS-15 is the producer of the protein feed additive. The strain was isolated in the process of long-term multistage selection by cultivation under aeration and stirring in a liquid mineral medium in an atmosphere of natural gas. Strain Methylococcus capsulatus GBS-15 is cultivated as part of an associative culture, including heterotrophic associates Cupriavidus gilardii, Stenotrophomonas acidaminiphila, Klebsiell apneumoni, which utilize the products of co-oxidation of methane homologues present in natural gas of Methylcoccus C, and metabolic products of the main methane strain, Methylloc.
  • the resulting protein feed additive is a dried inactivated biomass of the association of methane-oxidizing and heterotrophic bacteria.
  • Protein feed additive in the form of powder or granules is dosed in the required amount, which is determined by the norms of use in the preparation of feed mixtures.
  • the protein feed additive has a high nutritional value and is harmless to fish, animals and poultry.
  • Strain Methylococcus capsulatus GBS-15 and its associates are non-pathogenic, non-toxic and harmless.
  • the amount of protein in the protein feed additive is equivalent to animal proteins (fish meal and meat and bone meal). This allows widespread use protein feed additive as a substitute for fish meal and soybean meal in the composition of feed rations.
  • the biological value of the protein feed additive for chemical Scor is 65-70%.
  • the limiting amino acid is methionine.
  • the relative biological value to casein on the tetrachimenapyriformis test organism is 65-75%.
  • GAPRIN was used as a control food in all examples.
  • the protein feed additive can be added to fish feed without disturbing the amino acid balance of the feed.
  • the proposed mixture of strains has a high level of productivity, which contributes to an increase in the rate of food consumption of fish and the rate of weight gain.
  • Tests of the biological product in the diets of pigs were carried out at different age periods and on different age and gender groups:
  • Soybean meal was included as protein sources in the control diets.
  • a dietary supplement was included in the amount of up to 20% of the daily rate of digestible protein in the diet. Animals were weighed individually before testing, at the end of each growing period. The physiological state and health of the animals throughout the experiment were normal. No negative effects were found throughout the experiment. For the entire period of testing, no differences were noted in the health status of animals, feed consumption and the value of average daily gains in experimental and control animals.
  • the level of protein feed additive in the experimental group was 20% of the daily rate of digestible protein.
  • the nutritional value of the diets corresponded to generally accepted norms for the corresponding age of the animals and was identical for the control and experimental groups.
  • the main physiological indicators in the control and experimental groups as a result of the experiment were: the safety of chickens in the experimental group was 99.2%, in control group 98.8%. The intensity of egg production in the experimental group is 79.4%, in the control group 72.4%.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Polymers & Plastics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Physiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

La présente invention est destinée à l'élevage des animaux et à la pisciculture, et concerne notamment des additifs alimentaires biologiques pour la nourriture pour animaux dans le cadre de l'élevage d'animaux de ferme et de la pisciculture dans des réservoirs artificiels afin d'augmenter leur rendement ainsi que la résistance naturelle. Le résultat technique issu de l'utilisation de cet additif alimentaire pour animaux consiste en la création d'une technique efficace de production d'additif, et de meilleures propriétés d'utilisation de l'additif du fait d'une meilleur rendement des animaux et des poissons d'élevage. Ce résultat technique est obtenu grâce à l'utilisation d'un additif alimentaire protéiné pour animaux destiné à des animaux de ferme ou des poissons d'élevage, qui comprend des sources de bactéries oxydant le méthane Methylococcus capsulatus (GBS-15) VKM В-12549. Cet additif comprend en outre des souches de bactéries hétérotrophes Cupriavidus gilardii (GBS-15-1) VKM B-3265D et/ou des souches de bactéries hétérotrophes Stenotrophomonas acidaminiphila (GBS-15-2) VKM B-3264D et/ou des souches de bactéries hétérotrophes Klebsiella pneumonia 1-17 «VKPM– Obolensk» В-8465, prises dans des quantités égales selon le rapport suivant des composants en % en poids: souches de bactéries oxydant le méthane Methylococcus capsulatus VKM В-12549: 85 - 94; mélange de souches: 6 -15.
PCT/RU2020/050121 2019-07-22 2020-06-15 Additif alimentaire protéiné pour animaux de ferme et poissons d'élevage Ceased WO2021015648A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2021575092A JP7618246B2 (ja) 2019-07-22 2020-06-15 家畜及び魚の飼料タンパク質添加物
EA202091614A EA202091614A1 (ru) 2019-07-22 2020-06-15 Белковая кормовая добавка для сельскохозяйственных животных и рыб

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2019123037A RU2717991C1 (ru) 2019-07-22 2019-07-22 Белковая кормовая добавка для сельскохозяйственных животных и рыб
RU2019123037 2019-07-22

Publications (2)

Publication Number Publication Date
WO2021015648A2 true WO2021015648A2 (fr) 2021-01-28
WO2021015648A3 WO2021015648A3 (fr) 2021-05-14

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PCT/RU2020/050121 Ceased WO2021015648A2 (fr) 2019-07-22 2020-06-15 Additif alimentaire protéiné pour animaux de ferme et poissons d'élevage

Country Status (4)

Country Link
JP (1) JP7618246B2 (fr)
EA (1) EA202091614A1 (fr)
RU (1) RU2717991C1 (fr)
WO (1) WO2021015648A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024008734A1 (fr) * 2022-07-08 2024-01-11 Unibio Tech Science A/S Aliment pour ruminants

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2054881C1 (ru) * 1995-06-29 1996-02-27 Акционерное общество открытого типа "Кстовский опытно-промышленный завод белково-витаминных концентратов" Способ получения кормовой белковой добавки
AUPP816899A0 (en) * 1999-01-14 1999-02-11 Food Technology Innovations Pty Limited Improved microbial products
RU2159287C1 (ru) * 2000-04-03 2000-11-20 Государственное унитарное предприятие "Государственный научно-исследовательский институт биосинтеза белковых веществ" Способ получения белковой кормовой добавки
RU2613365C1 (ru) * 2016-04-07 2017-03-16 Ооо "Гипробиосинтез" Штамм метанокисляющих бактерий Methylococcus capsulatus ГБС-15 для получения микробной белковой массы
US11439172B2 (en) * 2017-07-03 2022-09-13 Oakbio, Inc. Microbial biomass based feed products
RU2652836C1 (ru) * 2017-08-02 2018-05-03 Общество с ограниченной ответственностью "БИОТРОФ" (ООО "БИОТРОФ") Кормовая добавка с пробиотической активностью для сельскохозяйственных животных, птиц, лошадей и рыб
RU2687135C1 (ru) * 2018-10-02 2019-05-07 Общество с ограниченной ответственностью "ГИПРОБИОСИНТЕЗ" Штамм гетеротрофных бактерий Cupriavidus gilardii - ассоциант для получения микробной белковой массы
RU2687136C1 (ru) * 2018-10-11 2019-05-07 Общество с ограниченной ответственностью "ГИПРОБИОСИНТЕЗ" Штамм гетеротрофных бактерий Stenotrophomonas acidaminiphila GBS-15-2 - ассоциант для получения микробной белковой массы
RU2687137C1 (ru) * 2018-10-11 2019-05-07 Общество с ограниченной ответственностью "ГИПРОБИОСИНТЕЗ" Штамм гетеротрофных бактерий Klebsiella pneumonia - ассоциант для получения микробной белковой массы

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024008734A1 (fr) * 2022-07-08 2024-01-11 Unibio Tech Science A/S Aliment pour ruminants

Also Published As

Publication number Publication date
EA202091614A1 (ru) 2021-05-31
JP2022541726A (ja) 2022-09-27
RU2717991C1 (ru) 2020-03-27
WO2021015648A3 (fr) 2021-05-14
JP7618246B2 (ja) 2025-01-21

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