[go: up one dir, main page]

WO2021007939A1 - Dispositif et procédé de collecte de cellules - Google Patents

Dispositif et procédé de collecte de cellules Download PDF

Info

Publication number
WO2021007939A1
WO2021007939A1 PCT/CN2019/105047 CN2019105047W WO2021007939A1 WO 2021007939 A1 WO2021007939 A1 WO 2021007939A1 CN 2019105047 W CN2019105047 W CN 2019105047W WO 2021007939 A1 WO2021007939 A1 WO 2021007939A1
Authority
WO
WIPO (PCT)
Prior art keywords
sampling material
cell
cell sampling
delivery tube
push rod
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2019/105047
Other languages
English (en)
Chinese (zh)
Inventor
姚香怡
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO2021007939A1 publication Critical patent/WO2021007939A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements
    • A61B10/02—Instruments for taking cell samples or for biopsy

Definitions

  • the present invention relates to the field of medical equipment, in particular to a cell collection device and method.
  • the problem is that the number of cells collected in a single collection is too small, usually in the hundreds to thousands. The effective number is not enough for the next step of testing, experiments, etc., and the number of cells collected in a single collection is too small. The process is easy to cause a very strong pain, which is difficult for most people to accept.
  • the present invention aims to provide a cell collection device and method, which aims to obtain sufficient cells in a gentle manner for medical tests, experiments, etc.
  • a cell collection device which includes: a delivery tube, a push rod, a connecting line, and a cell sampling material, wherein: the delivery tube has a cavity inside and has holes at both ends; The cell sampling material is located in the cavity of the delivery tube and is placed on the top of the push rod for loosely spreading and grabbing cells after contact with liquid; the push rod is placed inside the cavity of the delivery tube , Pushing the cell sampling material to the outside of the conveying pipe; the connecting line has one end connected with the cell sampling material and the other end placed outside the conveying pipe for retrieving the cell sampling material.
  • a wrapper located inside the cavity of the delivery tube, placed on the top of the push rod, for carrying the cell sampling material.
  • the wrapping shell is used to wrap the cell sampling material, and release the cell sampling material after being dissolved in a liquid.
  • the part of the push rod placed inside the cavity of the delivery tube is threadedly connected with the delivery tube, and the cell sampling material is pushed to the outside of the delivery tube through rotation.
  • the cell sampling material includes a polymer microporous flexible material, which is used for fluffy diffusion after contact with the liquid of the organism, and fits the part to be tested of the organism, and grabs the part to be tested of the organism through the micropores Surface cells.
  • the fine polymer microporous flexible material includes flexible polyurethane foam
  • the density of the flexible polyurethane foam is 20kg/m 3 ⁇ 30kg/m 3
  • the pore diameter of the micropores is 25PPI ⁇ 35PPI.
  • the flexible polyurethane foam includes 45%-55% toluene diisocyanate, 15%-25% polyether polyol, 20%-30% polyether alcohol, silicone oil and water.
  • the cell sampling material further includes a color developing agent attached to the surface of the cell sampling material or/and dispersed in the cell sampling material for displaying after contact with the liquid of a specific part of the organism The color corresponding to the liquid in the specific part.
  • the color-developing agent includes an anthocyanin food coloring, which is used to develop pink or pink color after contact with gastric juice.
  • the embodiment of the present invention also provides a cell collection method, including: using any of the cell collection devices provided in the embodiments of the present invention, inserting a delivery tube into the organism; using a push rod to push the cell sampling material to the delivery The outside of the tube makes the cell sampling material diffuse and fluffy after contacting with the liquid of the part to be tested, and fits the part to be tested of the organism, grabbing the cells on the surface of the part to be tested of the organism; The cell sampling materials are retrieved.
  • the present invention has the following significant advantages: push the cell sampling material that can be loosely diffused to the part to be tested in the organism through the push rod, grab the cell, and then use the connecting wire to pull back the cell sampling material , Since the cell sampling material can fully contact the part to be tested, a sufficient amount of cells can be obtained.
  • the cell collection device has a simple structure and can be made very small, and the cell sampling material is very soft after being fluffy and spread, and does not cause pain. The entire cell collection process is simple, fast and efficient.
  • Figure 1 is a schematic structural diagram of a cell collection device provided in an embodiment of the present invention.
  • FIG. 2 is a schematic diagram of a state of fluffy diffusion of the cell sampling material provided in an embodiment of the present invention
  • FIG. 3 is a schematic diagram of the structure of the wrapping shell wrapping the cell sampling material provided in the embodiment of the present invention.
  • Figure 4 is a schematic flow chart of a cell collection method provided in an embodiment of the present invention.
  • Fig. 1 is a schematic structural diagram of a cell collection device provided in an embodiment of the present invention, which will be described in detail below with reference to the accompanying drawings.
  • An embodiment of the present invention provides a cell collection device, including: a delivery tube 5, a push rod 4, a connecting line 2, and a cell sampling material 3, wherein: the delivery tube 5 has a cavity inside and has holes at both ends; The cell sampling material 3 is located inside the cavity of the delivery tube 5, placed on the top of the push rod 4, and used to spread loosely and grab cells after contact with the liquid; the push rod 4 is placed in the The inside of the cavity of the delivery tube 5 is used to carry the cell sampling material 3 and push the cell sampling material 3 to the outside of the delivery tube 5; one end of the connecting line 2 is connected to the cell sampling material 3, The other end is placed outside the delivery tube 5 for retrieving the cell sampling material 3.
  • the delivery tube 5 can be cylindrical or cone-shaped, which is convenient to be inserted and placed in the organism. Usually, it can be inserted and placed in the organism’s mouth, anus, digestive tract, etc. .
  • the conveying pipe 5 has a cavity inside for placing other components. The two radial ends of the conveying pipe 5 have openings, and the openings communicate with the inner cavity.
  • the push rod 4 is directly or indirectly movably connected with the delivery pipe 5.
  • Indirect movable connection that is, corresponding parts are arranged between the push rod 4 and the conveying pipe 5, for example, rubber is arranged between the push rod 4 and the conveying pipe 5, so that the push rod 4 can move relative to the conveying pipe 5.
  • the push rod 4 can be partly placed inside the delivery tube 5 and partly placed outside the delivery tube 5.
  • the push rod 4 can push the cell sampling material 3 to the outside of the delivery tube 5
  • the cell sampling material 3 can be pushed into the inside of the organism, so that the cell sampling material 3 can be delivered to the part to be tested.
  • the push rod 4 is directly movably connected with the delivery tube 5.
  • the push rod 4, the part placed inside the cavity of the delivery tube 5, is threadedly connected with the delivery tube 5, and the cell sampling material 3 is pushed to the place by rotation.
  • the delivery pipe 5 outside.
  • the cell sampling material 3 is placed at the top of the push rod 4, that is, between the top of the push rod 4 and the opening at the corresponding end of the delivery tube 5.
  • the cell sampling material 3 does not need to be connected to the push rod 4 or the delivery tube 5. So that the push rod 4 pushes the cell sampling material 3 through the opening of the delivery tube 5 to the outside of the delivery tube 5.
  • one end of the connecting line 2 is connected to the cell sampling material 3, and the other end is placed outside the delivery tube 5.
  • the connecting line 2 is placed outside the delivery tube 5 This end is outside the organism, and the cell sampling material 3 can be retrieved after the cell sampling material 3 completes cell sampling.
  • the connecting line 2 can be cotton thread, etc., and the retracting process will not cause pain to the body.
  • the cell sampling material 3 can be made of a flexible material that will fluffy and diffuse after contact with liquids and biological body fluids. Therefore, after the cell sampling material 3 is pushed to the outside of the conveying tube 5, it will interact with the body fluids of the organism. After the liquid is in contact, it can spread loosely and fully contact the internal surface of the organism. The surface of the cell sampling material 3 can rub against the internal surface of the organism to achieve grabbing and collecting cells on the internal surface of the organism.
  • the cell sampling material 3 is usually soft and flexible. The material does not cause pain to the body, and after full contact with the internal surface of the organism, a sufficient amount of cells can be obtained, which can meet the requirements of medical tests and experiments.
  • FIG. 2 is a schematic diagram of a state in which the cell sampling material 3 provided in an embodiment of the invention is fluffy and diffused.
  • the cell sampling material 3 includes a polymer microporous flexible material, which is used for fluffy diffusion after contact with the liquid of the organism, fits the part of the organism to be tested, and grabs the organism through the micropores. The cells on the surface of the part to be tested.
  • the polymer microporous flexible material can achieve fluffy diffusion after contact with liquid, and adhere to the body to be tested.
  • the micropores of the polymer microporous flexible material can be used to grasp and collect the cells on the surface of the test part after being attached to and rubbed against the body to be tested. Because the polymer microporous flexible material can be loosely diffused It fully fits the large area of the part to be tested, so it can also grab and collect enough cells for medical tests and experiments without causing pain in life.
  • the fine polymer microporous flexible material includes flexible polyurethane foam
  • the density of the flexible polyurethane foam is 20kg/m 3 ⁇ 30kg/m 3
  • the pore diameter of the micropores is 25PPI ⁇ 35PPI
  • the flexible polyurethane foam plastic includes 45%-55% toluene diisocyanate, 15%-25% polyether polyol, 20%-30% polyether alcohol, silicone oil and water.
  • the flexible polyurethane foam can be loosely diffused after contact with liquids and biological body fluids, and fully contact the part to be tested. Setting the density of the flexible polyurethane foam to 20kg/m 3 -30kg/m 3 can achieve a better effect of fluffy diffusion of the cell sampling material 3, and a better choice is to set it to 25kg/m 3 .
  • the pore size of the micropores of the flexible polyurethane foam is set to 25PPI ⁇ 35PPI (25 ⁇ 35 pores per square inch) (PPI, par per inch), which can achieve better scratching, grabbing and collecting the cells on the surface of the test part A better choice is to set it to 30PPI.
  • the content of toluene diisocyanate in the flexible polyurethane foam is set at 45%-55%, and a more preferable choice is set at 50%.
  • the content of the polyether polyol in the flexible polyurethane foam is set at 15%-25%, and a more preferable choice is set at 20%.
  • the content of polyether alcohol in the flexible polyurethane foam is set to 20%-30%, and a more preferable choice is to set it to 25%.
  • silicone oil and water are used to form the foaming crosslinking agent, and both require only a small amount, 1% to 3%. Other materials can be added to the flexible polyurethane foam.
  • FIG. 3 is a schematic diagram of the structure of the wrapper 1 that wraps the cell sampling material 3 provided in an embodiment of the present invention.
  • the cell collection device may further include: a wrapper 1 located inside the cavity of the delivery tube 5 and placed on the top of the push rod 4 to carry the cell sampling material 3.
  • the wrapping shell 1 is placed on the top of the push rod 4, that is, between the top of the push rod 4 and the opening at the corresponding end of the delivery tube 5.
  • the wrapping shell 1 does not need to be connected to the push rod 4 or the delivery tube 5 to facilitate The push rod 4 pushes the package shell 1 through the opening of the delivery pipe 5 to the outside of the delivery pipe 5.
  • the wrapping shell 1 is used to wrap the cell sampling material 3 and release the cell sampling material 3 after being dissolved in liquid.
  • the wrapping shell 1 is used to wrap the cell sampling material 3 to prevent the cell sampling material 3 from being contaminated, causing infection of organisms, or accidental contact with liquid. It is difficult for the cell sampling material 3 to restore the original state after contact with the liquid , Thus resulting in waste of the entire device.
  • the packaging shell 1 can be made of a material that is soluble in liquids, such as bioprotein glue. After being dissolved in the liquid, the cell sampling material 3 inside the packaging shell 1 can contact biological liquids and body fluids. The connecting wire 2 can pass through the wrapping shell 1 and connect with the cell sampling material 3.
  • the cell sampling material 3 further includes a color developing agent attached to the surface of the cell sampling material 3 or/and dispersed inside the cell sampling material 3 to interact with specific parts of the organism. After contacting with the liquid, it will show the color corresponding to the liquid in the specific part.
  • the color developing agent may be attached to the surface of the cell sampling material 3, or may be dispersed inside the cell sampling material 3, that is, in the micropores.
  • the corresponding color developer can be selected. For example, if the part to be tested is the stomach, the material of the color developer can show the corresponding color after contact with gastric juice. For example, if the part to be tested is the esophagus, select color The material of the agent can show the corresponding color after contact with saliva or body fluids in the esophagus.
  • the cell sampling material 3 After the cell sampling material 3 is pulled back through the connecting line 2, it can be judged whether the cell sampling material 3 has reached the part to be tested according to the color displayed by the color developer, so as to avoid the error of the cell collection position, which may lead to subsequent medical tests and experimental results mistake.
  • the chromogenic agent includes anthocyanin food coloring, which is used to develop pink or pink color after contact with gastric juice.
  • anthocyanin food coloring can be used when the part to be tested is the stomach. After the anthocyanin food coloring contacts the gastric juice, it can show a pink or pink color, and when it comes in contact with the anthocyanin food coloring Other liquids of the living body and other body fluids show different colors, so it can be determined whether the cell sampling material 3 reaches the part of the stomach.
  • the cell collection device has a simple structure and can be made very small.
  • the cell sampling material 3 is very soft after being fluffy and diffused, and does not cause pain.
  • the entire cell collection process is simple, fast and efficient.
  • FIG. 4 is a schematic flowchart of a cell collection method provided in an embodiment of the present invention.
  • An embodiment of the present invention provides a cell collection method, including: S1, using any one of the cell collection devices provided in the embodiments of the present invention, inserting the delivery tube 5 into the organism; S2, using the push rod 4 to collect the cell sampling material 3 Push to the outside of the delivery tube 5, so that the cell sampling material 3 will be loosely diffused after contacting with the liquid of the part to be tested, and fit the part of the organism to be tested, and grab the surface of the part of the organism to be tested through the micropores S3, by pulling the connecting line 2, the cell sampling material 3 is retrieved.
  • the method further includes: pulling the push rod 4 from the part to be tested in the organism, The part to be tested pulls out the delivery tube 5.
  • the method further includes: on the surface of the cell sampling material 3 or/and dispersed on the cell according to preset The color displayed by the color developer inside the sampling material 3 is used to determine whether the cell sampling material 3 is pushed to the part to be tested in the organism.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medical Informatics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

La présente invention se rapporte à un dispositif et à un procédé de collecte de cellules. Le dispositif comprend : un tube de distribution, une tige de poussée, un fil de raccordement et un matériau d'échantillonnage de cellules, le tube de distribution étant pourvu à l'intérieur d'une cavité et ayant des trous à deux extrémités ; le matériau d'échantillonnage de cellules est situé à l'intérieur de la cavité du tube de distribution, est disposé au niveau de l'extrémité supérieure de la tige de poussée, et devient pelucheux et se dilate après avoir été mis en contact avec un fluide de façon à capturer des cellules ; la tige de poussée est disposée à l'intérieur de la cavité du tube de distribution et est utilisée pour pousser le matériau d'échantillonnage de cellule vers l'extérieur du tube de distribution ; et une extrémité du fil de raccordement est reliée au matériau d'échantillonnage de cellule, et l'autre extrémité de celui-ci est disposée à l'extérieur du tube de distribution, et est utilisée pour retirer le matériau d'échantillonnage de cellule. Selon la solution ci-dessus, le matériau d'échantillonnage de cellules peut établir un contact complet avec un site à tester, de façon à obtenir une quantité suffisante de cellules. Le dispositif de collecte de cellules a une structure simple, et peut être fabriqué de telle sorte que celui-ci est très petit. Le matériau d'échantillonnage de cellules est très mou après devenir pelucheux et se dilate, et ne provoque pas de douleur. L'ensemble du procédé de collecte de cellules est simple, rapide et efficace.
PCT/CN2019/105047 2019-07-12 2019-09-10 Dispositif et procédé de collecte de cellules Ceased WO2021007939A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201910627066 2019-07-12
CN201910627066.6 2019-07-12

Publications (1)

Publication Number Publication Date
WO2021007939A1 true WO2021007939A1 (fr) 2021-01-21

Family

ID=74047933

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2019/105047 Ceased WO2021007939A1 (fr) 2019-07-12 2019-09-10 Dispositif et procédé de collecte de cellules

Country Status (2)

Country Link
CN (2) CN112206009A (fr)
WO (1) WO2021007939A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021007939A1 (fr) * 2019-07-12 2021-01-21 姚香怡 Dispositif et procédé de collecte de cellules

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4803998A (en) * 1986-01-27 1989-02-14 Ncs Diagnostics, Inc. Swab retaining vial cap and method of use
CN1921803A (zh) * 2004-02-23 2007-02-28 伊西康公司 诊断拭子和活组织检查穿刺器,以及用在这些系统中的诊断帽
CN103690306A (zh) * 2014-01-07 2014-04-02 魏永祥 标本采样拭子
CN204654998U (zh) * 2015-05-26 2015-09-23 劳尔军 一种采样拭子
CN208715800U (zh) * 2018-08-20 2019-04-09 益善生物技术股份有限公司 口腔拭子保存运输装置

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1455107A (en) * 1973-02-15 1976-11-10 Denson J W Diagnostic tampon and the use thereof for collecting cellular material
DE2812709C2 (de) * 1978-03-23 1982-08-19 Battelle-Institut E.V., 6000 Frankfurt Vorrichtung zur Gewinnung von Zellmaterialien aus Körperhöhlen
WO1991004710A1 (fr) * 1989-09-29 1991-04-18 Langdon Enterprises Incorporated Methode et appareil pour recueillir un prelevement cytologique
JP3079657B2 (ja) * 1991-07-11 2000-08-21 富士写真光機株式会社 細胞採取具
GB0415277D0 (en) * 2004-07-07 2004-08-11 Colonix Ltd Apparatus and method for sampling mucosal surfaces
US20160081670A1 (en) * 2014-09-18 2016-03-24 Covidien Lp Scored retaining features in a compressible cell collection device
CN207821846U (zh) * 2017-06-19 2018-09-07 苏州阅微基因技术有限公司 一种脱落细胞采集棒
WO2021007939A1 (fr) * 2019-07-12 2021-01-21 姚香怡 Dispositif et procédé de collecte de cellules

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4803998A (en) * 1986-01-27 1989-02-14 Ncs Diagnostics, Inc. Swab retaining vial cap and method of use
CN1921803A (zh) * 2004-02-23 2007-02-28 伊西康公司 诊断拭子和活组织检查穿刺器,以及用在这些系统中的诊断帽
CN103690306A (zh) * 2014-01-07 2014-04-02 魏永祥 标本采样拭子
CN204654998U (zh) * 2015-05-26 2015-09-23 劳尔军 一种采样拭子
CN208715800U (zh) * 2018-08-20 2019-04-09 益善生物技术股份有限公司 口腔拭子保存运输装置

Also Published As

Publication number Publication date
CN112206009A (zh) 2021-01-12
CN112206010A (zh) 2021-01-12

Similar Documents

Publication Publication Date Title
JP7261400B2 (ja) 胃腸サンプルを採取するためのデバイス
US4735214A (en) Gastrointestinal diagnostic capsule and method of use
US3502069A (en) Method and apparatus for placing in and retrieving a tubular probe from a body cavity
US4232673A (en) Method for collecting and processing body fluids
US3500819A (en) Medical probe adapted to be everted under pressure and method
CN110018013A (zh) 一种医用取样、检测一体化装置
FR2483213A1 (fr) Trousse de diagnostic et son utilisation dans un procede de diagnostic
CN101754721A (zh) 用于从腔体吸取物体的装置
WO2017181878A1 (fr) Sac d'extraction d'objets pour endoscope
US4471782A (en) Medical implement for use in rectum and method for inserting same
JP4643097B2 (ja) 生理学的標本採取装置
WO2021007939A1 (fr) Dispositif et procédé de collecte de cellules
CN209952027U (zh) 一种不易脱落的造影管
CN211066769U (zh) 一种临床消化检查管具
WO2021232394A1 (fr) Dispositif d'enrichissement de cellules rejetées de tube digestif
CN209464016U (zh) Tct检查取样装置
JP2006514847A (ja) 物質を制御可能に放出するための埋め込み可能な医療用装置
CN117017195A (zh) 一种胃肠外科用新型可调节扩肛器
EP0151583A1 (fr) Procede et appareil ameliores d'echantillonnage cytologique de l'endometre
CN117129671A (zh) 一种粪便样本采集及检测一体化装置
CN111973234B (zh) 一种体液提取器
CN211131151U (zh) 一种食道超声、阴道超声探头弹力保护套装置
CN223421286U (zh) 一种生物样品反应盒
CN107305164A (zh) 一种尿液中段采集装置
CN216294105U (zh) 一种改进的阴道探头专用膜套装置

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 19937933

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 19937933

Country of ref document: EP

Kind code of ref document: A1