WO2020231297A1 - Method of sorbing and removing heavy metal particles from the human body - Google Patents

Abstract

The invention relates to medicine, and more particularly to the sorption and removal of heavy metal particles and toxins from the human body using acupuncture. Acupuncture points are determined. At least 15 acupuncture needles are inserted at general acupuncture points of the body, and at least 15 acupuncture needles are inserted for localized acupuncture. The acupuncture needles are removed after 30-90 minutes. Said acupuncture needles comprise a handle and a rod, which are made of a metallic material. At least the working portion of the rod is covered with a coating based on magnetic nanoparticles of magnetite (Fe3O4) and/or maghemite (γ-Fe2O3). In one embodiment, the nanoparticle coating is applied by flame spraying. In another embodiment, the nanoparticles additionally comprise up to 20% silver and/or gold nanoparticles. In another embodiment, the size of the Fe3O4 nanoparticles is from 10 to 100 nm ± 20%. In another embodiment, the size of the γ-Fe2O3 nanoparticles does not exceed 20 nm. In another embodiment, a layer of magnetic nanoparticles of magnetite Fe3O4 and/or γ-Fe2O3 is additionally applied to the handle. In another embodiment, during application of the nanoparticle coating to the handle, the nanoparticles are applied in at least a double layer. Using this method makes it possible, as a result of the needle structure containing a coating based on magnetic particles of magnetite and/or maghemite, to reduce the concentration of harmful elements in the body.

Description

METHOD FOR SORPTION AND REMOVAL OF PARTICLES OF HEAVY METALS FROM

HUMAN ORGANISM

The present solution relates to the field of medicine, in particular, methods of sorption and removal of particles of heavy metals from the human body using

reflexology.

Today, there are many studies related to the study of the negative impact of heavy metal particles on the human body [1]. This situation is especially common for workers in the manufacturing industry, for example, the metallurgical industry.

Nickel compounds increase the risk of laryngeal cancer, cadmium compounds - prostate, hexavalent chromium - nasal cavity, arsenic - skin and hematopoietic tissue, lead - stomach, kidney and bladder, and mercury compounds - prostate and kidney. Arsenic skin cancer has long been known.

Occupational and iatrogenic arsenic skin cancer has been reported in contact with pesticides (in winegrowers), veterinary and medical disinfectants, and arsenic-containing ores.

The incidence of lung cancer is also increased in those in contact with arsenic. Chronic arsenic poisoning contributes to the emergence of rare tumors - angiosarcomas of the liver. Chromic cancer of the prostate and genitourinary organs has been described in workers in the dyeing industry, in metallurgy, electroplating, the tanning industry, in chemists, and in the production of incendiary mass for matches.

Nickel cancer develops in workers in the corresponding industries of non-ferrous

metallurgy, contact with nickel carbonyl used in the refining of this valuable metal is especially dangerous. Cobalt in the form of nitrite and in the composition of cobalt ore dust is also carcinogenic. Most often, its carcinogenic effect is combined with the effect of nickel, since it is extracted from the same ore.

Dust of beryllium compounds (oxide, fluoride, carbonate, nitrate), if inhaled, has a carcinogenic effect. Lung cancer with beryllium occurs with a very short, for an industrial carcinogen, latency period - 3 4 years. Experimentally, beryllium compounds can cause osteosarcomas (locally and distantly).

There is experimental evidence of the carcinogenic effect of mercury, silver, zinc, - causing sarcomas at the injection site, as well as epidemiological and experimental evidence of the role of cadmium oxide vapors and dust in the genesis of lung cancer.

Arsenic, nickel, cadmium and beryllium are present in cigarette tobacco and can (up to 10% of some of them) pass into tobacco smoke when smoked.

Nanoparticles are an intermediate link between atoms and bulk

molecules, and therefore are a promising area for research and various applications. The properties of nanoparticles differ from those of the same molecules.

Nanoparticles come in the form of one-dimensional nanostructures (nanotubes, nanorods, nanobels, nanors, nanobels, nanowires, nanofibers, nanospheres, nano flowers and nanosheets).

Among nanoparticles, nanomagnets occupy a special position due to their decent magnetic properties. Magnetic nanoparticles have a wide range of applications, including registration of magnetic fluids, catalysis, biotechnology / biomedicine, material sciences, magnetic resonance imaging [MRI], medical diagnostics, environmental rehabilitation. [2] - [7]

Through unique and creative applications in all walks of life, researchers are focused on developing a number of methods for synthesizing magnetic nanoparticles of different sizes, morphologies, and compositions, but the successful application of magnetic nanoparticles in the above examples is highly dependent on the stability of the particles. In addition, the application of magnetic nanoparticles in various fields depends on particle size, surface area, and morphology, in addition to their intrinsic magnetic moment and magnetocrystalline anisotropy.

Due to the nanoscale size of the magnetic nanoparticles, they can be attached to the cell, or they can be transported through the cell, entering the interior of the cell and may even directly enter the blood stream. Biomedical applications impose strict requirements on particle properties (physical, chemical pharmacological), including chemical composition, size, particle size uniformity, uniform crystal structure, magnetic properties, surface area and structure, adsorption properties, biocompatibility, hardness and flexibility, solubility, low toxicity and no allergic reactions.

Magnetic nanoparticles are widely used for drug administration due to their stability. They can efficiently and safely transfer the drug (with maximum load) to a specific site (site). The following parameters of nanomagnets are critical for use as drug carriers, they have the smallest particle size and larger surface, so that the deposition time is increased, and the surface characteristics of the magnetic nanoparticles protect them from degradation and make them excellent biocompatible drug delivery vehicles. They have excellent magnetic properties to reduce the concentration of nanomagnets in the blood and bring the drug to the target side in a timely manner.

Some of the problems associated with the use of magnetic nanoparticles include their in vivo system behavior. The efficacy of in vivo application prior to transfer into the target tissue depends on the ability of magnetic nanoparticles to cross the biological barriers of the vascular endothelium or the blood-brain barrier and to be recognized and cleared by the reticuloendothelial system (RES). Indirectly, the effectiveness of magnetic nanoparticles strongly depends on their size, morphology, charge chemistry, and surface. Several techniques, such as size reduction and non-fouling polymer injection, have been used to increase the efficiency of magnetic nanoparticles.

Next-generation magnetic nanoparticles for drug delivery include novel nanocrystalline cores, coating materials and functional ligands to improve the detection and specific delivery of nanoparticles. New core compositions of magnetic nanoparticles, such as doped iron oxide nanocrystals, metal / doped nanoparticles and nanocomposites, provide high magnetic moments that increase their signal-to-background ratio under magnetic resonance imaging. At the same time, the use of new surface coatings, such as stable gold or silica shell structures, allows the use of other toxic base materials, as well as more thorough coating of particles by forming self-assembled monolayers (SAMS) on the surface of nanoparticles. Known synthesis of hollow microspheres FC3O4 / S1O2 with FC3O4 as a shell (HMS @ PEG-PLA) for a targeted drug delivery process. This triple nanocomposite has advantages because of its hollow structure, which can load a large amount of drugs, because of its magnetic properties, it can be easily manipulated by applying an applied external magnetic field and because of the biodegradable and bioactive polymer shell of poly- (ethylene glycol) - poly- (D, L-lactide), it is biocompatible.

[eight]

In addition, recent studies and reviews show the increasing role of cell mechanics in diseases such as malaria and cancer metastases. In this way,

there is great potential for next generation platforms incorporating surface properties that will probe and / or monitor local physical and mechanistic changes at the length scale that would greatly contribute to improved disease detection, monitoring,

diagnosis and treatment.

Nanomagnets in the treatment of hyperthermia: Particles used for

magnetoprocessing therapy for hyperthermia includes grains such as rods of several mm (1-300 mm) and nanoparticles (1-100 nm). In the current clinical trials, a treatment for hyperthermia deserves attention, including

interstitial heating of targeted tumor cells followed by direct injection of magnetic nanoparticles into the target site. For the nanomagnetic treatment for prostate cancer, this clinical trial was applied in two separate phases. The first phase is only thermotherapy of applied magnetic nanoparticles, and the second phase is applied magnetotherapy of nanoparticles with continuous brachytherapy. This new approach requires specific tools for planning, quality control and thermal monitoring based on appropriate imaging and

modeling. Recently, multiphase magnetic composites have been successfully used for tunable magnetic systems in the treatment of hyperthermia to change the intrinsic magnetic properties, where the net contributions of various magnetic phases make it possible to modify the magnetization and anisotropy of the composite material. This approach assumes, for example, mixed phase composites

SrFei20i9 / MgFe204 / ZrCh that have been obtained, and the effective ability of magnetic treatment of hyperthermia. The magnetic properties of hard / soft composites SrFei20i9 / NiFe204 / ZnFe204, SrFei20i9 / ZnFe204 and SrFei20i9 / y-FerO3 have been studied. The results show that the exchange interaction between the hard and soft phases strongly affects the magnetization and coercivity of composites.

However, to date, the use of a coating based on nanoparticles of metal oxides has not been disclosed, in particular, magnetite (Bez0 ₄ ) and / or maghemite (y-BossOz), which is part of acupuncture needles to ensure sorption and removal of heavy metal particles from the human body.

The objective of the claimed invention is to create a new method of sorption of particles of heavy metals in the human body, followed by their removal, using acupuncture needles for reflexology.

The technical result for the method and device is a general improvement in human health by reducing the concentration of harmful elements in the body when using the claimed method. The sorption and removal of heavy metal particles from the human body is ensured through the use of a coating in the structure of the needle based on magnetic nanoparticles of iron oxides, magnetite (Fe3O3) and / or maghemite (g-FemO3).

The technical result is achieved in that the method of sorption and removal of particles of heavy metals from the human body using reflexotherapy includes: determining acupuncture points, introducing at least 15 needles for reflexology into general acupuncture points of the body and introducing at least 15 needles for

reflexotherapy locally, removal of the reflexotherapy needles after 30-90 minutes, and the said acupuncture needles for reflexotherapy contain a handle and a rod made of a metal material, while the working part of the rod is coated with a coating based on magnetic iron oxide nanoparticles.

As nanoparticles, BezOz and / or γ-BegOz particles can be used.

In one of the private embodiments, the coating of nanoparticles is applied

flame spraying.

In another particular embodiment, the nanoparticles additionally contain up to 20% silver and / or gold nanoparticles.

In another particular embodiment, the size of BezOz nanoparticles is from 10 to 100 nm ± 20%. In another particular embodiment, the size of γ-FerO3 nanoparticles does not exceed 20 nm.

In another particular embodiment, an additional layer of magnetic nanoparticles of iron oxide BdsOz and / or y-BszOz is applied to the handle.

In another particular embodiment, when a nanoparticle coating is applied to a needle handle, the nanoparticles are applied in at least two layers.

The technical result for the device is achieved in that the acupuncture needle, which is intended for use in the specified method, contains a handle and a rod containing a working part, made of a metal material, and a coating is applied to the working part of the rod based on magnetic nanoparticles of iron oxide BSZO3 and / or y- Running.

FIG. 1 shows a general view of an acupuncture needle. FIG. 2 - table 1.

To implement the proposed method, you can use the needle shown in Fig. 1. The needle contains a rod (1) made of metal, for example, stainless medical steel, and a handle (3) made of metal. On the working part of the rod (1) and / or handle (3), a layer (2) of magnetic nanoparticles of iron oxide, in particular, magnetite (Without OD and / or maghemite (y-BsOz) is applied. The ratio of oxide nanoparticles when used together in the composition layer (2) can be different, for example, 50/50, 20/80, 70/30, etc.

The layer of nanoparticles (2) can be applied, for example, using flame spraying. Nanoparticles are manufactured with the following dimensions: es0 ₄ - from 10 to 100 nm ± 20%; y-RunOs - up to 20 nm.

On the working part of the needle (1), a layer of nanoparticles (2) is applied, as a rule, in one layer. When applied to the handle (3), a layer of nanoparticles (2) is applied in at least two layers.

Additionally, nanoparticles can contain up to 20% silver and / or gold nanoparticles, which increases the efficiency of disinfection. For example, colloidal silver particles and / or colloidal gold particles.

See Table 1 for examples of acupuncture needles. The dimension of the nanoparticles of iron oxides is verified experimentally and the method of obtaining the particles is given in the sources of information, in particular, those indicated in the "Background Art" section.

The amount of application of additional particles of silver and / or gold is required to increase the bactericidal effect. The optimal ratio of the quantitative addition of these particles was obtained experimentally and showed that at around 20% of particles taken individually or in aggregate (silver / gold) gives a persistent positive effect. When this amount was exceeded, the effect did not change, as a result of which the optimal value indicated in the application was obtained.

The use in the claimed method of the design of the needles according to the above examples allows you to achieve the claimed technical effect with various variations of their implementation within the requested scope of legal protection and to provide

effective sorption of heavy metals from the body.

Poisoning with all heavy metals is characterized by approximately the same symptoms. The digestive tract will be the first to react to acute poisoning (violation of peristalsis, pain, nausea, vomiting). As heavy metals are absorbed into the blood, reactions from the heart and blood vessels (pressure surges, shortness of breath), kidneys and liver are connected. Irreversible consequences for the body occur, as in the case of Minamata disease, when a heavy metal affects the nervous system.

In order to study the work of nanoparticles in the composition of the claimed layer (2) applied to the needles (10), studies were carried out on a group of 50 people. Biochemical studies were carried out with patients of acupuncture medical centers, as well as workers of metallurgical enterprises. Studies have shown that upon contact of needles (10) for 30 minutes with the patient's lymph and / or blood flow, the initial biochemical index of heavy metals in the human body decreased. The decrease is due to the fact that nanomagnetic particles adsorbed a certain amount of heavy metals through needles (10).

This sorption contributed to a decrease in their amount in the human body.

Reduction of heavy metals by sorption through needles (10) contributed to

the effectiveness of conservative treatment of heavy metal poisoning led to a faster therapeutic effect. Also, this sorption contributed to a decrease in the level of heavy metals in the human body for preventive purposes - removal from the body of heavy metals received by humans in production, as well as in everyday life. In all cases, the control biochemical analysis showed a decrease in the level of heavy metals in the human body.

From the group of participants, the following changes were characteristic:

Participant N ° l.

The patient was taken to the infectious diseases department of one of the hospitals with suspected poisoning with inherent symptoms (nausea, vomiting, stomach pain). Selected analysis for the presence of heavy metals showed a large amount of nickyl carbonyl. The selected analysis for the presence of heavy metals showed a large amount of nickyl carbonyl. The placement of needles (10) in the acupuncture points of the stomach and intestines (10) for 30 minutes, subsequently, according to the results of repeated analyzes, showed a significant decrease in nickyl carbonyl. Needles (10) were also placed in acupuncture points not only locally, but also throughout the body to trap heavy metals from the blood and lymph flow of the body.

Participant N ° 2.

The patient complained of persistent pain in the area of the elbow and shoulder joints. The analyzes revealed an increased content of nickel carbonyl particles. For 5 days, one hour sessions of acupuncture needles were conducted in the area of pain localization (10). After applying these manipulations, a decrease in nickyl carbonyl was observed, as well as the passage of pain. Needles (10) were also placed in

acupuncture points not only locally, but also for the whole body to capture heavy metals from the bloodstream and lymph flow of the body.

Participant N ° 1 and N ° 2 were employees of a metallurgical plant.

Participant N ° 3.

The patient complained of hearing loss. The diagnosis indicated impaired functioning of the auditory nerve. The analysis showed an increased amount of mercury particles in the body. The use of acupuncture needles (10) in the ears in combination with medical physiotherapy, conservative treatment, made it possible to achieve positive dynamics of hearing restoration. Needles (10) were also placed in acupuncture points not only locally, but also throughout the body to trap heavy metals from the blood and lymph flow of the body.

Participant N ° 4. The patient had complaints related to implicit skin lesions in the shoulder area. Analysis showed that these areas of the skin had lesions with pores of mercury.

The classical treatment did not lead to positive dynamics, since mercury vapors were in the layers of the epidermis. The use of acupuncture needles (10) led to the removal of mercury particles from the deep layers of the epidermis, which in combination with

conservative treatment has led to significant positive results. Needles (10) were also placed in acupuncture points, not only locally, but throughout the body, to capture heavy metals from the bloodstream and lymph flow of the body.

Participant N ° 5.

The patient was a long-term smoker and complained of persistent cough and shortness of breath. The analysis showed a significant presence of such heavy metal salts as: Arsenic, nickel, cadmium and beryllium. After weekly sessions

reflexology using needles (10) improved the patient's condition, led to a decrease in these heavy metals in the body. The excretion of stagnant sputum from the lungs was also improved, general health improved, chronic cough

decreased. Needles (10) were also placed in acupuncture points not only locally, but also throughout the body to trap heavy metals from the blood and lymph flow of the body.

When using the claimed design of acupuncture needles (10), heavy metals are collected precisely on that part of the needle (10), which is located under the skin, that is, placed in the body. As a rule, the minimum number of needles installed in the general acupuncture points of the body is 15 needles, and about 15 needles are installed locally in one or another area of localization, as a result, blood and lymph flow, due to the created field, begins sorption. It should also be noted that the particles of heavy metals that are simply present in the lymph and blood are magnetized to the needle (10) and subsequently, together with the needle (10), are pulled out of the body.

Typically, an acupuncture session lasts 30 to 90 minutes, once a day. Used on average from 30 needles.

Sources of information:

Nikitina Yu.E. et al. Study of the effect of micro - and macroelements on the human body and bioaccumulation of some ions of heavy metals by microorganisms // Volsk Military Institute of Material Support, 2016 Gao, J., H. Gu, and B. Xu, Multifunctional magnetic nanoparticles: design, synthesis, and biomedical applications. Accounts of chemical research, 2009.42 (8): p. 1097-1107.

Xie, T., L. Xu, and C. Liu, Synthesis and properties of composite magnetic material SrCoxFel2-x019 (x = 0-0.3). Powder Technology, 2012. An, T., et ah, Synthesis of Carbon Nano tube- Anatase Tyu2 Sub-micrometer-sized Sphere

Composite Photocatalyst for Synergistic Degradation of Gaseous Styrene. ACS applied materials & interfaces, 2012.4 (11): p. 5988-5996.

Teymourian, H., A. Salimi, and S. Khezrian, Fe304 magnetic nanoparticles / reduced graphene oxide nanosheets as a novel electrochemical and bioeletrochemical sensing platform. Biosensors and B ioelectronic s, 2013.

Zhang, B., et ah, Microwave absorption enhancement of Fe304 / poly aniline core / shell hybrid microspheres with controlled shell thickness. Journal of Applied Polymer Science, 2013.

Rashad, M. and I. Ibrahim, Structural, micro structure and magnetic properties of strontium hexaferrite particles synthesized by modified coprecipitation method. Materials Technology: Advanced Performance Materials, 2012.27 (4): p. 308-314.

Deng, H. and Z. Lei, Preparation and characterization of hollow Fe304 / Si02 @ PEG-PLA nanoparticles for drug delivery. Composites Part B: Engineering, 2013.

Claims

CLAIM 1. A method of sorption and removal of particles of heavy metals from the human body using reflexology, including: determining acupuncture points, introducing at least 15 reflexotherapy needles into acupuncture points of the whole body and introducing at least 15 reflexotherapy needles into acupuncture points locally in accordance with the affected organs, removal of the needles for reflexology after 30-90 minutes, and the mentioned acupuncture needles for reflexology contain a handle and a rod made of metal material, while at least the working part of the rod is coated with magnetic nanoparticles of iron oxide magnetite BszO3 and / or maghemite g-BszOz. 2. The method according to claim 1, characterized in that in the needles used, the coating of the nanoparticles is applied by flame spraying. 3. The method according to claim 2, characterized in that the nanoparticles used in the needles additionally contain up to 20% of silver and / or gold nanoparticles. 4. The method according to claim 1, characterized in that the size of the Res04 nanoparticles in the needles used is from 10 to 100 nm ± 20%. 5. The method according to claim 1, characterized in that in the needles used, the size of g-FerO3 nanoparticles does not exceed 20 nm. 6. A method according to claim 1, characterized in that in the needles used, the metal material is stainless steel. 7. The method according to claim 1, characterized in that in the needles used a layer of magnetic nanoparticles of iron oxide Bs Oz and / or g-BszOz is applied to the handle. 8. A method according to claim 7, characterized in that in the needles used, the layer is applied to the handle in at least two layers.