WO2020258169A1 - Method for dyeing sample, device for preparing smear, and dye solution combination - Google Patents

Abstract

The present invention relates to a method for dyeing a sample, a device for preparing a smear, and a dye solution combination. The dye solution combination includes a first dye solution and a second dye solution, wherein the first dye solution is a mixture of a first biological dye and a second biological dye, wherein the first biological dye can dye acidophilic substances in the sample, and the second biological dye can dye basophilic substances in the sample; and the second dye solution is different from the first dye solution, and the second dye solution is a mixture of a third biological dye and a buffer solution, wherein the third biological dye can redye the basophilic substances or the acidophilic substances in the sample in order to strengthen the dyeing effect. The sample dyeing method comprises first immersing a sample in the first dye solution for dyeing, and then immersing the sample in the second dye solution for dyeing, wherein during the whole immersion process, neither the first dye solution nor the second dye solution will fail in the short term, and this is conducive to the repeated use of the first dye solution and the second dye solution and saves on dyeing costs.

Description

Sample staining method, smear preparation equipment and staining solution combination Technical field

This application relates to the field of medical testing, and specifically to a method of staining samples.

Background technique

Sample staining is a very important step in the process of sample detection and analysis. Taking glass slides as an example, the traditional glass slide staining method is basically done manually, which is inefficient. Therefore, there are many automated dyeing instruments. These automated dyeing instruments generally use the vertical dip dyeing method. The dyeing process is basically imitated by manual operation, usually in a small container. The blood film is pre-fixed with pure dye solution, and then the dye solution and buffer solution are prepared in a certain proportion. Mix the liquid to soak the slides. After a period of time, suck off the mixed solution and discard it, and then repeatedly add deionized water to rinse.

Summary of the invention

technical problem

However, because the mixed solution of the dye solution and the buffer solution fails within a short period of time, such as usually about ten minutes, precipitation occurs, no longer has a dyeing effect, and cannot be recycled, resulting in great waste. Although the small and medium-sized containers of the existing automatic dyeing instruments are usually made into thin slices to reduce the volume, the volume is still significantly larger than the manual method due to the influence of the glass slide area, so that the dyeing cost is still high.

The solution to the problem

Technical solutions

This application mainly provides a novel sample dyeing method, smear preparation equipment and dyeing solution combination.

An embodiment of the present application provides a sample staining method, including:

Selection steps: select the slides coated with samples;

Dyeing step: soak the sample in a dyeing tank and then take it out. The dyeing tank stores a first dye solution. The first dye solution is a mixture of a first biological dye and a second biological dye. The first biological dye can Dyeing the acidophilic substance in the sample, and the second biological dye can dye the alkaliphilic substance in the sample;

Counter-staining step: soak the sample in a counter-staining tank and take it out. The counter-staining tank stores a second dye solution. The second dye solution is different from the first dye solution. The second dye solution is the second dye solution. A mixture of three biological dyes and buffers, where the third biological dye can stain basophils or acidophils in the sample;

The method further includes: selecting the next glass slide coated with the sample, and repeating the staining step to the counter staining step.

In one embodiment, it further includes a staining promotion step: immersing the sample processed in the staining step in a staining accelerating tank and then taking it out, where a buffer is stored;

The dye accelerating step is located after the dyeing step and before the counterstaining step.

In an embodiment, in the stain promotion step, the sample is soaked in the buffer for 1-5 minutes.

In one embodiment, it further includes a pre-counter-dyeing cleaning step: immersing the sample processed in the dye accelerating step in a cleaning tank and then taking it out, the cleaning tank storing a cleaning liquid.

In one embodiment, it further includes a cleaning step before counter-dyeing: cleaning the sample with a cleaning liquid, which is stored in a cleaning tank;

The washing step before counterstaining is located after the dyeing step and before the counterstaining step.

In an embodiment, in the pre-counter-staining washing step, the sample washing time is 1-5 minutes.

In an embodiment, the second dye solution further includes a first biological dye, the third biological dye is a basic biological dye, and the volume ratio of the first biological dye is much smaller than that of the third biological dye Volume ratio.

In an embodiment, the first biological dye is eosin dye, the second biological dye is methylene blue dye, and the third biological dye is azure dye.

In an embodiment, the first dye solution is Wright-Giemsa stain, the second dye solution is a mixture of Giemsa stain and buffer, or the second dye solution is Liu’s B stain Mixture of liquid and buffer.

In an embodiment, in the second dye solution, the volume ratio a of the Giemsa dye or Liu's B dye and the buffer solution is 1/30≤a≤1/10.

In an embodiment, in the counterstaining step, the soaking time of the sample is 5-15 minutes.

In an embodiment, in the dyeing step, the soaking time of the sample is 1-5 minutes.

In an embodiment, it further includes a second cleaning step: cleaning the sample after the counterstaining step.

In an embodiment, it further includes:

When the control unit determines that there is no sample to perform the dyeing step or receives the first dye solution recovery instruction issued by the user, it recovers the first dye solution in the dyeing tank to the first dye solution sealed container for storage, and waits. One-time use

When the control unit determines that there is a sample that needs to perform the dyeing step or receives the first dye liquor input instruction issued by the user, the first dye liquor stored in the first dye liquor sealed container is re-sent to the dyeing tank, To reuse the first dye solution.

In an embodiment, it further includes:

When the control unit determines that there is no sample to perform the dyeing step or receives an instruction to cover the first dye solution issued by the user, the control unit covers the first dye solution in the dyeing tank and waits for the next use;

When the control unit determines that there is a sample that needs to perform the dyeing step or receives a user's instruction to open the first dye solution, it uncovers the dyeing tank to reuse the first dye solution.

In an embodiment, the method for the control unit to determine that there is no sample to perform the staining step includes: there is no sample in the staining tank and no sample waiting to be sent, and/or the idle time of the staining tank exceeds a set value.

In an embodiment, after the control unit determines that the first dye liquor meets the set discharge requirement or receives the first dye liquor discharge instruction issued by the user, the first dye liquor is discharged.

In an embodiment, the discharge requirement includes that the ratio of the number of times of dyeing the sample to the volume of the first dye solution is more than 2 times/ml and/or the use time of the first dye solution is more than 2 days.

In an embodiment, it further includes:

When the control unit determines that there is no sample to perform the counterstaining step or receives the second dyeing solution recovery instruction issued by the user, the second dyeing solution in the counterstaining tank is recovered to the second dyeing solution recovery container for storage, Waiting for the next use;

When the control unit determines that there is a sample that needs to be subjected to the counter-staining step or receives a second dye solution input instruction issued by the user, the second dye solution stored in the second dye solution recovery container is re-sent to the counter dye Tank to reuse the second dye solution.

In an embodiment, it further includes:

When the control unit determines that there is no sample to perform the counter-staining step or receives an instruction to cover the second dye solution issued by the user, the control unit covers the second dye solution in the counter-staining tank and waits for the next use;

When the control unit determines that there is a sample that needs to be subjected to the counter-staining step or receives an instruction to open the second dye solution from the user, the cover of the counter-dye tank is released to reuse the second dye solution.

In an embodiment, the method for the control unit to determine that there is no sample to perform the counterstaining step includes: there is no sample in the counterstaining tank and the dyeing tank and no sample to be sent, and/or idle time of the counterstaining tank Exceeds the set value.

In an embodiment, when the control unit determines that the second dye solution meets the set discharge requirement or receives the second dye solution discharge instruction issued by the user, the second dye solution is discharged.

In an embodiment, it further includes:

When the control unit determines that there is no sample to perform the pre-counter-dyeing cleaning step or receives the cap cleaning liquid instruction from the user, cap the cleaning liquid in the cleaning tank and wait for the next use;

When the control unit determines that there is a sample that needs to perform the pre-counter-staining cleaning step or receives an instruction to open the cleaning liquid from the user, the cleaning tank is uncovered to reuse the cleaning liquid.

In an embodiment, it further includes:

When the control unit determines that there is no sample to perform the dye accelerating step or receives an instruction to cap the dye accelerating solution from the user, cap the buffer in the dye accelerating tank and wait for the next use;

When the control unit determines that there is a sample that needs to perform the staining step or receives an instruction to open the staining solution issued by the user, the control unit uncovers the staining tank to reuse the buffer solution.

In one embodiment of the present application, a smear preparation device is provided, which includes a containing tank, a conveying mechanism, and a control unit. The containing tank includes:

A dyeing tank for storing a first dye solution, the first dye solution is a mixture of a first biological dye and a second biological dye, the first biological dye can dye acidophilic substances in the sample, and the second The biological dye can dye the alkaliphilic substance in the sample, and the dyeing tank has a first opening through which the glass slide coated with the sample can pass;

A counterstaining tank for storing a second dye solution that is different from the first dye solution. The second dye solution is a mixture of a third biological dye and a buffer solution. Basophils are dyed, and the counterstaining tank has a second opening through which the slide can pass;

The transfer mechanism is used for picking up the glass slide and driving the glass slide to move;

The control unit outputs a control signal to control the conveying mechanism to drive the sample to be immersed in the dyeing tank for dyeing, and then immersed in the counter dyeing tank for counter dyeing.

In one embodiment, it further includes a first dye solution liquid path control system and a first dye solution sealed container, and the first dye solution liquid path control system is respectively communicated with the first dye solution sealed container and the dyeing tank to connect The corresponding first dye solution in the dyeing tank is recovered to the first dye solution sealed container, and the first dye solution in the first dye solution sealed container is discharged into the dyeing tank again.

In an embodiment, the first dye solution liquid path control system includes a first dye solution recovery system and a first dye solution inlet system, and the first dye solution recovery system is sealed with the first dye solution in a sealed container and dyeing solution respectively. The tanks communicate with each other to recover the corresponding first dye solution in the dyeing tank to the first dye solution sealed container; the first dye solution inlet system communicates with the first dye solution sealed container and the dye tank, respectively, for The first dye solution in the first dye solution sealed container is discharged into the dyeing tank again.

In one embodiment, the first dye solution recovery system includes a first dye solution recovery pipeline and a first dye solution recovery pressure source, and the first dye solution recovery pipeline is sealed with the dye tank and the first dye solution respectively The container is in communication, and the first dye solution recovery pressure source sucks the first dye solution in the dyeing tank into the first dye solution sealed container;

The first dye liquor inlet system includes a first dye liquor inlet pipe and a first dye liquor inlet pressure source, and the first dye liquor inlet pipe is respectively connected with the dyeing tank and the first dye liquor sealed container , The first dye liquor inlet pressure source discharges the first dye liquor in the sealed container of the first dye liquor into the dyeing tank.

In an embodiment, the first dye liquor recovery pipeline and the first dye liquor inlet pipeline are the same pipeline, and/or the first dye liquor inlet pressure source and the first dye liquor recovery The pressure source is the same pressure source.

In an embodiment, when the control unit determines that there is no sample to be dyed or receives the first dye solution recovery instruction issued by the user, the first dye solution path control system controls the corresponding first dye solution in the dyeing tank. The dye liquor is recovered to the first dye liquor sealed container; when the control unit determines that a sample needs to be dyed or receives the first dye liquor instruction from the user, the first dye liquor path control system will The first dye solution in the first dye solution sealed container is discharged into the dyeing tank again.

In an embodiment, the first dye solution sealed container is a reagent bottle, and the reagent bottle is arranged inside the smear preparation device.

In an embodiment, it further includes a first dye solution cover. When the control unit determines that there is no sample to perform dyeing work or receives an instruction from the user to cover the first dye solution, the first dye solution cover covers the dyeing Corresponding openings on the tank, and reopen the dyeing tank when needed.

In one embodiment, the method for the control unit to determine that there is no sample to be dyed includes: there is no sample in the dyeing tank and no sample to be dyed is fed, and/or the idle time of the dyeing tank exceeds a set value.

In an embodiment, it further includes a first dye liquor discharge system, which is in communication with the dyeing tank or the first dye liquor sealed container, and when the control unit determines that the first dye liquor meets the discharge requirements or After receiving the first dye solution discharge instruction issued by the user, the first dye solution discharge system discharges the first dye solution in the dyeing tank or the first dye solution sealed container.

In one embodiment, the discharge requirement includes: the ratio of the number of times of dyeing the sample to the volume of the first dye solution is more than 2 times/ml and/or the use time of the first dye solution is more than 2 days.

In an embodiment, it further includes a second dye solution liquid path control system and a second dye solution recovery container. The second dye solution liquid path control system is in communication with the second dye solution sealed container and the counter dye tank for The corresponding second dye solution in the counter dyeing tank is recovered to the second dye solution recovery container, and the second dye solution in the second dye solution recovery container is discharged into the counter dyeing tank again.

In an embodiment, the second dye solution liquid path control system includes a second dye solution recovery system and a second dye solution inlet system, the second dye solution recovery system and the second dye solution recovery container and counterstaining The tanks communicate with each other to recover the corresponding second dye solution in the counter dye tank to the second dye solution recovery container; the second dye solution inlet system communicates with the second dye solution recovery container and the counter dye tank to The second dye solution in the second dye solution recovery container is discharged into the counter dyeing tank again.

In one embodiment, the second dye solution recovery system includes a second dye solution recovery pipeline and a second dye solution recovery pressure source, and the second dye solution recovery pipeline is connected to the counter dye tank and the second dye solution respectively. The recovery container is in communication, and the second dye solution recovery pressure source sucks the second dye solution in the counter dye tank into the second dye solution recovery container;

The second dye liquor inlet system includes a second dye liquor inlet pipe and a second dye liquor inlet pressure source, and the second dye liquor inlet pipe is respectively connected to the counter dye tank and the second dye liquor recovery container Connected, the second dye liquor inlet pressure source discharges the second dye liquor in the second dye liquor recovery container into the counter-dye tank.

In an embodiment, the second dye liquor recovery pipeline and the second dye liquor inlet pipeline are the same pipeline, and/or the second dye liquor inlet pressure source and the second dye liquor recovery The pressure source is the same pressure source.

In an embodiment, when the control unit determines that there is no sample to perform counter-dyeing work or receives a second dye solution recovery instruction issued by the user, the second dye solution circuit control system controls the corresponding The second dye liquor is recovered to the second dye liquor recovery container;

When the control unit determines that there is a sample that needs to be re-stained or receives a second dye liquor input instruction issued by the user, the second dye liquor path control system recovers the second dye liquor in the container The second dye solution is discharged into the counter dye tank again.

In one embodiment, it further includes a second dye solution cover. When the control unit determines that no sample needs to be counter-dyeing or receives an instruction from the user to cover the second dye solution, it controls the second dye solution cover to cover the place. The counterstaining tank; when the control unit determines that there is a sample that needs counterstaining work or receives a user's instruction to open the second dyeing solution, it controls the second dyeing solution cover to open the counterstaining tank.

In an embodiment, the accommodating tank further includes a dye accelerating tank for storing buffer, the dye accelerating tank has a third opening for the slide to pass through; the control unit outputs a control signal to control the The conveying mechanism drives the sample to sequentially move to the dyeing tank for soaking for dyeing, the dyeing accelerating tank for accelerating dyeing, and the counter-staining tank for soaking for counterstaining.

In an embodiment, the containing tank further includes a washing tank for storing cleaning liquid, the washing tank has a fourth opening for the glass slide to pass through; the sample is in the stain accelerating tank to complete the staining Afterwards and before counterstaining in the counterstaining tank, the control unit outputs a control signal to control the conveying mechanism to drive the sample to move into the cleaning tank for cleaning.

In one embodiment, the dyeing tank, dye accelerating tank, cleaning tank and counter-dyeing tank are arranged in a row in sequence.

In an embodiment, the accommodating tank further includes a cleaning tank for storing cleaning liquid, the cleaning tank has a fourth opening for the glass slide to pass through; the control unit outputs a control signal to control the transfer The mechanism drives the sample to sequentially move to the dyeing tank for soaking for dyeing, the cleaning tank for cleaning, and the counterstaining tank for soaking for counterstaining.

In one embodiment, it further includes a cleaning liquid cover. When the control unit determines that no sample needs to be cleaned before counter-dyeing or receives a capping cleaning liquid instruction issued by the user, it controls the cleaning liquid cover to cover the corresponding cleaning tank. Opening, and reopen the cleaning tank when needed.

In one embodiment, it further includes a dye-promoting liquid cover. When the control unit determines that no sample needs to perform dye-promoting work or receives an instruction from the user to cover the dye-promoting liquid, it controls the dye-promoting liquid cover to cover the dye-promoting tank Corresponding opening, and reopen the dye accelerating tank when needed.

In an embodiment, it further includes a mounting base and a first drive assembly, the accommodating groove is mounted on the mounting base, the mounting base is in transmission connection with the first drive assembly, and the accommodating groove on the mounting base It moves under the drive of the first drive assembly.

In an embodiment, it further includes an integral outer cover for shielding the corresponding opening on the accommodating groove when the accommodating groove is in a non-use state.

In an embodiment, it further includes a mounting base and a first drive assembly, the accommodating groove is mounted on the mounting base, and any one of the integral outer cover and the mounting base is drivingly connected to the first drive assembly for Driven by the first drive assembly, the integral outer cover is moved relative to the containing grooves on the mounting seat, and the corresponding openings on each containing groove are covered.

In an embodiment, it further includes a liquid inlet system, and the liquid outlet of the liquid inlet system communicates with the containing tank for injecting corresponding liquid into the containing tank.

An embodiment of the present application provides a dyeing solution combination for sample dyeing, comprising a first dyeing solution and a second dyeing solution, the first dyeing solution being a mixture of the first biological dye and the second biological dye, The first biological dye can dye the acidophilic substance in the sample, the second biological dye can dye the basophilic substance in the sample, and the second dye solution is a mixture of the third biological dye and a buffer solution. The third biological dye is capable of dyeing alkali or acidophilic substances in the sample.

In an embodiment, the first biological dye is eosin dye, the second biological dye is methylene blue dye, and the third biological dye is azure dye.

In an embodiment, the first dye solution is Wright-Giemsa stain, the second dye solution is a mixture of Giemsa stain and buffer, or the second dye solution is Liu’s B stain Mixture of liquid and buffer.

In an embodiment, in the second dye solution, the volume ratio a of the Giemsa dye or Liu's B dye and the buffer solution is 1/30≤a≤1/10.

In an embodiment, the second biological dye and the third biological dye are the same biological dye.

According to the above embodiments, a first dye solution and a second dye solution are provided. The first dye solution is a mixture of the first biological dye and the second biological dye. The first biological dye can dye the acidophilic substance in the sample, and the second Biological dyes can stain alkaliphiles in samples. The second dye solution is different from the first dye solution. The second dye solution is a mixture of the third biological dye and the buffer solution. The third biological dye can counter-stain the alkali or acidophilic substances in the sample to enhance the dyeing effect. Soak the sample in the first dye solution for dyeing, and then soak the sample in the second dye solution for dyeing. During the entire immersion process, the first dye solution and the second dye solution will not fail in a short period of time, which is beneficial The first dye solution and the second dye solution are used repeatedly, saving dyeing costs.

The beneficial effects of the invention

Brief description of the drawings

Description of the drawings

1 and 2 are schematic diagrams of the structure of a smear preparation device in an embodiment of the application;

Figure 3 is a schematic diagram of a smear preparation process in an embodiment of the application;

4-7 are schematic diagrams of the steps of several different embodiments of the sample staining method of this application;

FIG. 8 is a schematic diagram of the structure of the accommodating groove in an embodiment of the application;

FIG. 9 is a schematic structural diagram of a sample staining device in an embodiment of the application;

FIG. 10 is a schematic structural diagram of a sample staining device in another embodiment of the application.

Invention embodiment

Embodiments of the invention

Hereinafter, the present invention will be further described in detail through specific embodiments in conjunction with the drawings. Among them, similar elements in different embodiments use related similar element numbers. In the following embodiments, many detailed descriptions are used to make the present application better understood. However, those skilled in the art can easily realize that some of the features can be omitted under different circumstances, or can be replaced by other elements, materials, and methods. In some cases, some operations related to this application are not shown or described in the specification. This is to avoid the core part of this application being overwhelmed by excessive descriptions. For those skilled in the art, these operations are described in detail. The related operations are not necessary, they can fully understand the related operations according to the description in the manual and the general technical knowledge in the field.

In addition, the features, operations, or features described in the specification can be combined in any appropriate manner to form various implementations. At the same time, the steps or actions in the method description can also be exchanged or adjusted in order in a manner obvious to those skilled in the art. Therefore, the various orders in the specification and the drawings are only for the purpose of clearly describing a certain embodiment, and are not meant to be a necessary order, unless it is otherwise stated that a certain order must be followed.

The serial numbers assigned to the components herein, such as "first", "second", etc., are only used to distinguish the described objects and do not have any sequence or technical meaning. The "connection" and "connection" mentioned in this application include direct and indirect connection (connection) unless otherwise specified.

The inventor has conducted in-depth analysis and research on the causes of precipitation in the existing dyeing solution, and finally found that different dyes in the existing dyeing solution will undergo chemical reactions in the buffer environment, thereby causing precipitation. Take Wright-Giemsa dye as an example, because Wright-Jemsa dye contains two main dyes, eosin and methylene blue. When the Wright-Giemsa stain and the buffer are mixed, in the buffer system, the eosin dye is negatively charged, while the methylene blue dye is positively charged, the two will react to produce precipitation, and the process is irreversible As a result, the Wright-Giemsa stain and buffer mixture cannot be stored for a long time, and often needs to be discarded after one use, causing great waste.

In this regard, this embodiment provides a method for staining a sample, which is used for staining a sample. The sample includes, but is not limited to, microorganisms, blood, body fluids, and bone marrow fluid. In order to express the inventive concept of the present application more clearly, blood (glass slide) and a smear preparation device for making the slide are described below as an example.

Please refer to FIG. 1 and FIG. 2 in combination. In one embodiment, a smear preparation device is provided, which can be used for, but not limited to, smear preparation of samples such as microorganisms, blood, body fluid, and bone marrow fluid. The smear preparation equipment includes a sampling mechanism for taking samples, a slide loading mechanism for moving the slides to the work line, a dropping mechanism for loading samples on the slides, and a slide The slide pushing mechanism 4 for smoothing the sample, the drying mechanism (not shown in the figure) for drying the blood film on the slide, and the staining mechanism 5 for staining the slide.

Please refer to Figures 1 to 3. In one embodiment, when the sampling mechanism 1 performs sample extraction, the sample is mixed first, and then the sampling device (such as the sampling needle 31) in the sampling mechanism 1 is used to aspirate the sample. Different, aspiration can be a puncture aspiration (the sample container has a cover, and the sampling device passes through the cover of the sample container) or an open aspiration (the sample container is open, and the sampling device directly aspirates the sample from the open part). When necessary, blood sample information can be tested to obtain information and comparison information. In some embodiments, the micro-sampling mechanism 6 is further included. The micro-sampling mechanism 6 can directly move the test tube inserted by the operator in the direction of the sampling device, or the sampling device can also move toward the test tube inserted by the operator. Move in direction. In other implementations, the micro-injection mechanism 6 can also directly move the test tube in the direction of the sample drop mechanism 3, or the sample drop mechanism 3 can also move in the direction of the test tube inserted by the operator, directly via the sample drop mechanism 3 ( For example, a blood drip needle) draws a blood sample and then loads the sample. Since there is no need to draw blood through the sampling mechanism 1, the need for blood samples can be reduced, thereby realizing micro and priority injection. After the sampling is completed, the sample dropping mechanism 3 prepares to drop the blood onto the glass slide.

Correspondingly, the glass slide loading mechanism 2 extracts the glass slide and loads the glass slide to a corresponding position to facilitate the blood drip operation. Among them, as shown in FIG. 3, in some embodiments, after the glass slide extraction operation is completed, operations such as left and right inspection of the glass slide and glass slide cleaning can also be performed, and then the glass slide can be loaded to the set position. The loaded slide can be printed with relevant information, and at the same time perform operations such as positive and negative detection of the slide.

Please continue to refer to FIGS. 1 to 3, the blood dripping needle of the sample dripping mechanism 3 drops the sample onto the glass slide and then pushes the slide. The slide push mechanism 4 pushes the blood on the slide into a blood film shape. Usually, after the slide pushing action is completed, the blood film on the slide can be dried to stabilize its shape. As shown in FIG. 3, in some embodiments, the glass slide can be driven to turn over before the blood film is dried to meet corresponding requirements. In some embodiments, the dried glass slide can also be tested for drying to determine the effect of blood film drying. In some embodiments, the dried glass slide can also be tested for blood film expansion to determine whether the blood film is expanded and whether the expanded state meets the requirements.

Please continue to Figures 1 to 3, after pushing the slide, the slide (blood smear) can be stained (can be achieved by the staining mechanism 5) or output directly (for example, placed in the slide basket 7 for output).

For the above-mentioned smear preparation equipment or other devices related to sample staining, an embodiment of the present application provides a sample staining method.

Please refer to Figures 4, 9 and 10, the sample staining method includes:

Dyeing step S10: soak the sample in the dyeing tank 110 and then take it out. The dyeing tank 110 stores the first dye solution. The first dye solution is a mixture of the first biological dye and the second biological dye. The first biological dye can stain acidophilic substances in the sample, and the second biological dye can stain alkaliphilic substances in the sample.

Counter-staining step S30: the sample is soaked in the counter-staining tank 120 and then taken out, the counter-staining tank 120 stores the second dye solution. The second dye solution is different from the first dye solution, and the second dye solution is a mixture of the third biological dye and the buffer solution. The third biological dye can stain the alkali or acidophilic substances in the sample. Wherein, the third biological dye may be the same as or different from the first biological dye or the second biological dye.

In the staining step S10, the cytoplasm and nucleus of the sample can be stained separately, so as to form a desired effect. In an embodiment, in the dyeing step S10, the soaking time of the sample is 1-5 minutes (including 1 minute and 5 minutes, and the time range mentioned below includes the values at both ends). Taking into account the comprehensive factors such as work efficiency and dyeing effect, the soaking time can be set at 1-3 minutes.

In the dyeing step S10, that the first dye solution is a mixture of the first biological dye and the second biological dye means that the first dye solution mainly includes the first biological dye and the second biological dye, and it also Some auxiliary materials commonly used in this field may be added as needed, and these auxiliary materials are conventional means in this field. Here, it is not limited that the first dye solution contains only the two components of the first biological dye and the second biological dye.

Those skilled in the art can understand that the first biological dye capable of dyeing acidophilic substances in the sample in this embodiment means that the first biological dye can bind to the acidophilic substances in the sample to change color. For example, the acidophilic substance includes hemoglobin, eosinophilic particles, etc., the first biological dye may be an eosin dye, and the eosin dye is combined with the acidophilic substance to turn red. In the same way, that the second biological dye can dye the alkaliphilic substance in the sample means that the second biological dye can combine with the alkaliphilic substance in the sample to change color. For example, the basophil includes lymphocyte cytoplasm and basophilic particles, and the second biological dye may be a methylene blue dye, which can be combined with the basophil to become blue-violet.

The cell nucleus is mainly composed of DNA and basic protein. Basic protein is easy to combine with eosin to stain red. At the same time, there is a small amount of weak acidic substance in the nucleus, which combines with methylene blue to stain blue, but because the content is less, blue The color reaction is weak and the nucleus is stained purple. Azure, the oxide of methylene blue, can also play a similar role to methylene blue.

The sample is immersed in the first dye solution, which can immerse the carrier containing the sample (the glass slide with blood is used as an example for description below the glass slide) into the first dye solution and keep it for a certain period of time. The counterstaining step S30 can stain the nucleus or other places of the sample with acidophils or basophils again, and the addition of buffer is beneficial to improve the dyeing effect of the third dye on acidophils or basophils, and facilitate subsequent follow-ups. Detection and observation. In an embodiment, in the counterstaining step S30, the soaking time of the sample is 5-15 minutes. For example, it can be controlled at about 10 minutes.

In the counterstaining step S30, that the second dye solution is a mixture of the third biological dye and the buffer solution means that the second dye solution mainly includes the third biological dye and the buffer solution, and it may also be based on It is necessary to add some auxiliary materials commonly used in this field, and these auxiliary materials are conventional means in this field. Here, it is not limited that the second dye solution contains only the two components of the third biological dye and the buffer solution. The buffer refers to a liquid that can provide an ionization environment, such as a phosphate solution, and may even include an aqueous solution that can form an ionization environment.

The counterstaining step is used to strengthen the dyeing of alkaliphilic substances or acidophilic substances. The third biological dye can be selected according to actual needs to dye the alkaliphilic substances or acidophilic substances. For example, it can be combined with the first biological dye. The dye or the second biological dye are the same. Of course, the third biological dye can also choose a dye different from the first biological dye and the second biological dye. For example, the first biological dye is eosin dye and the second biological dye is methylene blue. Dye, the third biological dye may be an azure dye, which may be oxidized from methylene blue dye, and is used for dyeing alkaliphiles.

This dyeing method provides two dye solutions. Firstly, soak the sample in the first dye solution for dyeing. At this time, if no buffer is added to the first dye solution, there will be no occurrence between the first biological dye and the second biological dye. Chemical reaction, resulting in no precipitation. After the dyeing step is completed, the sample is immersed in the second dye solution for dyeing. At this time, only an acidic or alkaline biological dye (third biological dye) is added to the second dye solution for dyeing the sample. The inner basophils or acidophils are stained. The third biological dye alone cannot form a precipitate in the buffer environment. In the entire dyeing process, the first dye solution and the second dye solution will not fail in a short period of time, which is beneficial to the repeated use of the first dye solution and the second dye solution and saves dyeing costs. After the dyeing step S10, the carrier of the sample may carry a part of the first biological dye into the second dye solution, but this part of the first biological dye and the second biological dye is very small, not enough to form too much with the third biological dye The precipitation has little effect on the second dye solution.

Although, compared with the existing method, this embodiment uses two dye solutions, the first dye solution and the second dye solution, for dyeing, which increases the number of dye solutions, but because the first dye solution and the second dye solution can be repeated The use of immersed multiple glass slides has a long time effect. On average, the dyeing cost of each slide is greatly reduced.

Since the first dye solution and the second dye solution can be used repeatedly for a long time, in one embodiment, the method further includes the step of selecting the next glass slide coated with the sample, and repeating the above dyeing steps to counterstaining. step. This step can be repeated multiple times.

Further, referring to FIGS. 5, 9 and 10, in one embodiment, the dyeing method further includes a dye accelerating step S21: the sample after the dyeing step S10 is immersed in the dye accelerating tank 130 and then taken out, the dye accelerating tank Buffer is stored in 130;

The dyeing promotion step S21 is located after the dyeing step S10 and before the counterstaining step S30.

In the stain promotion step S21, the buffer can further promote the staining efficiency of the first biological dye and the second biological dye in the sample to the sample, and improve the staining effect. At the same time, the buffer not only promotes staining, but also cleans. The first biological dye and the second biological dye attached to the glass slide after the accelerating step S21 are less, which is beneficial to reduce the chemical reaction between the first biological dye and the second biological dye in the subsequent counterstaining step S30.

In an embodiment, in the stain accelerating step S21, the sample is soaked in the buffer for 1-5 minutes, for example about 2 minutes.

Further, referring to FIGS. 6, 9 and 10, in one embodiment, it also includes a pre-counter-dyeing cleaning step S22: the sample processed in the dye accelerating step S21 is immersed in the cleaning tank 140 and then taken out, the cleaning tank 140 Stored with clean liquid;

The pre-counter-staining washing step S22 is located after the accelerating step S21 and before the counter-staining step S30.

In this step, the cleaning liquid can be a buffer solution or a cleaning solution, etc., so as to soak or clean the sample. In the pre-counter-staining washing step S22, the sample is washed with a buffer solution or a washing solution to further reduce the first biological dye and the second biological dye carried on the glass slide. After this step is processed, in the counter-staining step S30 The glass slide will hardly bring the first biological dye and the second biological dye into the second dye solution, avoiding the chemical reaction of the first biological dye, the second biological dye and the third biological dye to produce precipitation, making the second dye solution It can be used for a longer time, further reducing dyeing costs. During cleaning, the sample can be immersed in a cleaning solution or buffer solution, or the sample can be rinsed with a cleaning solution or buffer solution.

Wherein, in the pre-counter-staining cleaning step S22, the sample can be cleaned with a buffer solution or a cleaning solution once or more, depending on the cleaning effect and requirements. The cleaning fluid can be deionized water and other commonly used cleaning fluids in this field. Of course, a buffer solution can also be used to clean the sample, which can further promote the dyeing of the sample by the dye.

The cleaning time depends on the cleaning effect and the purpose to be achieved. In one embodiment, in the pre-counter-staining cleaning step S22, the sample cleaning time is 1-5 minutes.

In another embodiment, as shown in Figs. 7, 9 and 10, the dye-promoting step S21 may not be performed, but after the dyeing step S10 and before the counter-dyeing step S30, the counter-dyeing cleaning step S22 is performed. At this time, in the pre-counter-staining cleaning step S22, the sample is cleaned with a cleaning liquid, and the cleaning liquid is stored in the cleaning tank 140. In this step, the cleaning liquid can be either a cleaning liquid or a buffer solution (in this case, the repetitive The pre-dyeing step S22 is equivalent to the aforementioned dyeing promotion step S21).

The cleaning step S22 before counterstaining can wash off the first biological dye and the second biological dye carried on the glass slide, so as to prevent the first biological dye and the second biological dye from being carried into the second dyeing solution and interact with the third biological dye. Produce precipitation.

Similarly, the cleaning time depends on the cleaning effect and the purpose to be achieved. In one embodiment, in the pre-counter-staining cleaning step S22, the cleaning time of the sample is 1-5 minutes.

As a more specific embodiment, the third biological dye is a basic biological dye, which can dye alkaliphilic substances in the sample, such as methylene blue or azure dye. The second dye solution also includes the first biological dye, and the volume ratio of the first biological dye is much smaller than the volume ratio of the third biological dye, so as to avoid the chemical reaction between the two and the precipitation.

Specifically, the first biological dye is eosin dye, and the second biological dye is methylene blue dye. Of course, the first biological dye and the second biological dye can also be replaced with other dyes that can be dyed with both alkali and acidophils, respectively.

Further, in order to meet the usage habits of most clinical departments, in one embodiment, the first stain is Wright-Giemsa stain, and the Wright-Giemsa stain (referred to as Wright's stain) includes Eosin dye and methylene blue dye. The second dye solution is a mixture of Giemsa dye solution and buffer solution, and the Giemsa dye solution contains methylene blue dye as the third biological dye (that is, the third biological dye is the same as the second biological dye).

Wright's dye solution is made by mixing basic dye Methylene Blue and acid dye Eosin Y, also called eosin-methylene blue dye. Among them, the colored part of eosin is an anion, the colorless part is a cation, and the colored part is acid, so eosin is customarily called an acid dye. Methylene blue is usually a chloride salt which is alkaline, the colored part is a cation, and the colorless part is an anion, just the opposite of eosin.

After Wright’s staining solution is stored for a long time, the methylene blue in it contains azure due to oxidation. Methylene blue, azure and eosin can stain the cell nucleus into purple, but cannot stain the cytoplasm into blue. The methylene blue can stain the cytoplasm blue.

The slides stained by this method are very close to the traditional Wright-Giemsa staining solution manual staining method, doctors accept a high degree, and do not change the habits of microscopists, so it is easy to promote.

Wherein, the second dye solution can also be replaced with a mixture of other dye solution with methylene blue dye and buffer solution, such as Liu's B dye solution.

Further, in order to improve the dyeing effect in the counterstaining step S30, in the second dyeing solution, the volume ratio a of the Giemsa dye or Liu's B dye and the buffer is set to a value of 1/30≤a≤1/10.

Preferably, in an embodiment, the volume ratio a of the Giemsa stain or Liu's B stain and the buffer in the second stain is 1/25.

Further, referring to Figs. 4-7, in one embodiment, it further includes a second cleaning step: cleaning the sample after the counter-staining step S30.

Similarly, the cleaning of the sample in this step can be performed with a cleaning solution or a buffer solution. Common cleaning methods such as soaking or flushing can also be used for cleaning.

The use of the above dyeing method can not only greatly reduce the user’s dyeing cost, but also because the reagents in each step can be used repeatedly, the timeliness is very long, and it can be stored in the instrument for a long time without moving, so the liquid path structure becomes very simple. In extreme cases, it is not even necessary to support the liquid path. Only the user fills the dyeing tank with reagents, and the manufacturing cost of the entire instrument will become very low, which is very conducive to the promotion of low-end customers.

The sample processed by the above-mentioned dyeing method can continue to be dried and exported, and these steps can all be realized by using the existing technology, which will not be repeated here.

Furthermore, since this embodiment provides two dye solutions, during the entire dyeing process, neither the first dye solution nor the second dye solution will fail in a short period of time, and the first dye solution and the second dye solution are used repeatedly.

In an embodiment, please refer to FIG. 10, which also includes:

When the control unit determines that there is no sample to perform the dyeing step or receives the first dye solution recovery instruction issued by the user, the first dye solution in the dyeing tank 110 is recovered to the first dye solution sealed container 710 for storage, waiting for the next use ；

When the control unit determines that there is a sample that needs to be dyed or receives the first dye solution input instruction issued by the user, the first dye solution stored in the first dye solution sealed container 710 is re-sent to the dyeing tank 110, To reuse the first dye solution.

Since the first dye solution usually uses high-purity methanol as a solvent, it has a very strong volatility. If it is exposed to the air, it will quickly evaporate. The above steps can recycle and seal the first dye solution when not in use to avoid volatilization and prolong its service life.

Of course, in addition to being recycled to the first dye solution sealed container 710 for sealed storage when not in use, it also includes other methods.

For example, in an embodiment, it further includes:

When the control unit determines that there is no sample to perform the dyeing step or receives an instruction from the user to cover the first dye solution, it covers the first dye solution in the dyeing tank 110 and waits for the next use;

When the control unit determines that there is a sample that needs to be dyed or receives an instruction from the user to open the first dye solution, the dyeing tank 110 is uncovered to reuse the first dye solution.

The cover can be driven by a corresponding driving structure, and one or two methods will be introduced below.

The above two methods are only an example of the sealed preservation of the first dye solution, and the two methods can be selected and used according to the actual structure and requirements. The two methods can not only reduce the volatilization of the first dye solution, but also prevent some sundries in the equipment from falling into the first dye solution and avoid contamination of the first dye solution.

Regarding the timing of recovery or capping, in one embodiment, the method for the control unit to determine that no sample needs to be dyed includes, but is not limited to: no sample in the dyeing tank 110 and no sample waiting to be sent, and/or the dyeing tank 110 The idle time exceeds the set value.

For the first dye solution of the present application, since it can be used repeatedly, its replacement frequency is greatly reduced. In the simplest way, the user can manually replace the first dye solution when it needs to be replaced. Of course, in order to improve work efficiency and further realize automation, in one embodiment, when the control unit determines that the first dye solution meets the set discharge requirement or receives the first dye solution discharge instruction issued by the user, the first dye solution emission. The discharge can be through the corresponding first dye liquor discharge system. The first dye liquor discharge system will be further introduced below. The discharged first dye liquor can be collected and processed uniformly.

The discharge requirements of the first dye solution can be set according to actual needs. In one embodiment, the discharge requirements include, but are not limited to: the ratio of the number of times the sample is dyed to the volume of the first dye solution reaches 2 times/ml or more And/or the use time of the first dye solution reaches more than 2 days. For example, when 50 ml of the first dye solution is stored in the dyeing tank, the first dye solution can be discharged after the sample has been dyed in the first dye solution for more than 100 times. The number of staining times is not absolutely equal to the number of stained samples. When the sample completes a complete staining action in the first staining solution, it is a staining. Due to different staining requirements or staining effects, the same sample may be in the first staining solution Perform multiple staining. When the first dye solution meets the above requirements, the first dye solution can be discharged and replaced manually or automatically by the user.

Further, the second dye solution, cleaning solution, and dye accelerating solution can also be separately provided with a recovery system or capping means to achieve recycling.

In an embodiment, please refer to FIG. 10, which also includes:

When the control unit determines that there is no sample to perform the counterstaining step or receives the second dyeing solution recovery instruction issued by the user, the second dyeing solution in the counterstaining tank 120 is recovered to the second dyeing solution recovery container 720 for storage, and waiting One-time use

When the control unit determines that there is a sample that needs a counterstaining step or receives a second dyeing solution feed instruction issued by the user, the second dyeing solution stored in the second dyeing solution recovery container 720 is sent to the counterstaining tank 120 again, To reuse the second dye solution.

In addition, in an embodiment, it may also include:

When the control unit determines that there is no sample to perform the counterstaining step or receives an instruction to cover the second dyeing solution from the user, the second dyeing solution in the counterstaining tank 120 is covered and waiting for the next use;

When the control unit determines that there is a sample that needs a counter-staining step or receives an instruction to open the second dye solution from the user, the counter-dye tank 120 is uncovered to reuse the second dye solution.

Through the above method, the second dye solution can be recycled to avoid the volatilization of the second dye solution. At the same time, it can also prevent some sundries in the equipment from falling into the first dye solution, avoiding the pollution of the first dye solution, thereby improving The service life of the second dye solution.

Among them, in one embodiment, the method for the control unit to determine that no sample needs to be counterstained includes but is not limited to: there is no sample in the counterstaining tank 120 and the dyeing tank 110 and no sample is to be sent, and/or the counterstaining tank 120 idle time exceeds the set value.

In the same way, for the second dye solution of the present application, since it can be used repeatedly, its replacement frequency is greatly reduced. In the simplest way, the user can manually pick and place the second dye solution when it needs to be replaced. Of course, in order to improve work efficiency and further realize automation, in one embodiment, when the control unit determines that the second dye solution meets the set discharge requirement or receives the second dye solution discharge instruction issued by the user, the second dye solution emission.

The discharge requirement can be determined with reference to the discharge requirement of the first dye solution, for example, from the ratio of the number of times of dyeing the sample to the volume of the second dye solution and/or the number of days the second dye solution is used. Of course, other emission requirements are also feasible.

Similarly, for the cleaning liquid and buffer as the cleaning liquid, please refer to FIG. 10. In an embodiment, it further includes:

When the control unit determines that there is no sample to perform the pre-counter-dyeing cleaning step or receives the cap cleaning liquid instruction issued by the user, the cleaning liquid in the cap cleaning tank 140 is capped and waited for the next use;

When the control unit determines that there is a sample that needs to be cleaned before counter-dyeing or after receiving an instruction to open the cleaning liquid from the user, the cleaning tank 140 is uncovered to reuse the cleaning liquid.

As for the buffer solution in the stain promotion step, in an embodiment, it further includes:

When the control unit determines that there is no sample to perform the dye-promoting step or receives the instruction of capping the dye-promoting liquid from the user, it caps the buffer in the dye-promoting tank 130 and waits for the next use;

When the control unit determines that there is a sample that needs to perform a staining step or receives a user's instruction to open the staining solution, it uncovers the staining tank 130 to reuse the buffer solution.

Since the volatility of the cleaning solution and the buffer solution is lower than that of the first dye solution and the second dye solution, the recovery of the above cleaning solution and buffer solution can be selected according to the situation. In addition, the discharge and replacement of the cleaning solution and the buffer solution can also refer to the aforementioned processing methods of the first dye solution and the second dye solution, which can be realized manually or automatically by the user.

Please refer to FIGS. 8-10. An embodiment of the present application also provides a sample dyeing device, which is applied to a smear preparation device, and includes a receiving tank 100, a transfer mechanism 200, and a control unit (not shown in the figure). The sample staining device can be used as a sample staining solution module of smear preparation equipment or other related equipment.

The accommodating tank 100 includes a dyeing tank 110 for storing the first dye solution and a counter dyeing tank 120 for storing the second dye solution.

The first dye solution can be the first dye solution defined above. The staining tank 110 has a first opening through which a carrier with a sample can pass. The second dye solution can also be the second dye solution defined above. The counterstaining tank 120 has a second opening through which the carrier can pass.

The conveying mechanism 200 is used to pick up the slide 500 containing the sample and drive the slide 500 to move. The control unit outputs a control signal, and the transport mechanism is controlled to drive the sample to be immersed in the dyeing tank 110 for dyeing (the above-mentioned dyeing step S10 is completed), and then immersed in the counter-dyeing tank 120 for counterstaining (the above-mentioned counter-staining step S30 is completed).

Among them, the transmission mechanism 200 can use various power sources as power sources, such as motors, air cylinders, hydraulic cylinders, electromagnetic drives, etc., to drive the slide 500 to move. In an embodiment, please refer to FIGS. 9 and 10, the transfer mechanism 200 includes a pickup assembly 210 for picking up the slide 500 and a first driving assembly 220 (the pickup assembly 210 and the first driving assembly 220 shown in FIG. 9 are Simple schematic diagram), the first driving assembly 220 is in transmission connection with the picking assembly 210 to drive the picking assembly 210 to move.

As mentioned above, the first driving assembly 220 can be driven by a motor, an air cylinder, a hydraulic cylinder, an electromagnetic drive, and the like. The pickup assembly 210 can adopt various types of manipulator structures, and can pick up and release the glass slide 500. The first drive assembly 220 drives the pickup assembly to move. For example, as shown in FIG. 9, the first drive assembly 220 drives the pickup assembly 210 to move along the y-axis and z-axis, thereby driving the slide 500 to change position in these two directions for movement. Go to the position of each containing tank 100 for soaking. Alternatively, as shown in FIG. 10, the first driving assembly 220 drives the pickup assembly 210 to move along the x-axis, y-axis and z-axis.

Further, referring to FIGS. 9 and 10, in an embodiment, the containing tank 100 further includes a dye accelerating tank 130 for storing buffer solution, and the dye accelerating tank 130 has a third opening for the glass slide 500 to pass through. After the sample is dyed in the dyeing tank 110 and before the counterstaining is performed in the counterstaining tank 120, the control unit outputs a control signal, and controls the conveying mechanism to drive the sample to move to the dye accelerating tank 130 for accelerating and cleaning (complete the aforementioned accelerating dyeing Step S21).

Further, referring to FIGS. 9 and 10, in an embodiment, the accommodating tank 100 further includes a washing tank 140 for storing washing liquid or buffer, and the washing tank 140 has a fourth opening for the glass slide 500 to pass through; After the sample is dyed and cleaned in the dye accelerating tank 130 and before the counter dye in the counter dye tank 120, the control unit outputs a control signal, and the transfer mechanism 200 is controlled to drive the sample to move to the cleaning tank 140 for cleaning (complete the above counter dye Pre-cleaning step S22).

In order to improve the convenience of operation and increase the dyeing efficiency, as shown in Figures 9 and 10, in one embodiment, the dyeing tank 110, the dye accelerating tank 130, the cleaning tank 140 and the counter dyeing tank 120 are arranged in a row, for example Arrange along the y axis. The pick-up assembly 210 can sequentially pass through each accommodating slot 100 to perform corresponding operations, and complete each step in sequence. This arrangement can make the structure of the sample staining device more compact, and can shorten the movement stroke of the pickup assembly 210, and improve the staining efficiency. It can be understood that the dyeing tank 110, the dye accelerating tank 130, the washing tank 140, and the counter-dyeing tank 120 may be integrally formed or separately provided.

In another embodiment, the dye accelerating groove 130 may not be provided. The accommodating tank 100 also includes a washing tank 140 for storing a cleaning solution. The washing tank 140 has a fourth opening for the glass slide 500 to pass through; after the sample is dyed in the dyeing tank 110 and the counterstaining tank 120 is re-stained Before dyeing, the control unit outputs a control signal to control the transport mechanism 200 to drive the sample to move into the cleaning tank 140 for cleaning (complete the pre-counter-dyeing cleaning step S22). The washing tank 140 can be directly arranged between the dyeing tank 110 and the counter-dyeing tank 120.

In the foregoing embodiments, the number of the cleaning tank 140 may be one or more, so that the buffer or cleaning solution is used to clean the sample once or more, depending on the cleaning effect and requirements.

Further, the accommodating tank 100 further includes a second cleaning tank 150 for storing a cleaning solution or a buffer solution, and the second cleaning tank 150 has a fifth opening for the glass slide 500 to pass through. After the sample is counterstained in the counterstaining tank 120, the control unit outputs a control signal to control the transport mechanism 200 to drive the sample to move into the cleaning tank 140 for cleaning (the second cleaning step S50 is completed).

Further, please refer to FIG. 8, in an embodiment, part or all of the accommodating tank 100 (including the dyeing tank 110, the counter-dyeing tank 120, the dye accelerating tank 130, the cleaning tank 140, and the second cleaning tank 150) The structure shown in Figure 8 can be used. The accommodating tank 100 stores corresponding liquids (such as the first dye solution, the second dye solution, buffer solution or cleaning solution, etc.) and can be immersed in the glass slide 500 coated with the sample. The accommodating groove 100 includes at least one first receiving part 101, and the first receiving part 101 forms an insertion area 102 for inserting a glass slide. Corresponding (such as dyeing) treatment is performed by immersing the glass slide 500 placed on the first placing part 101 in the containing tank 100 in a liquid (such as a dye solution) for a certain period of time.

Further, referring to FIGS. 9 and 10, in an embodiment, the dyeing tank 110, the counter-dyeing tank 120, the dye accelerating tank 130, the cleaning tank 140, and the second cleaning tank 150 are arranged in sequence. There is more than one pickup assembly 210. For example, as shown in FIG. 10, there are two picking assemblies 210, which can work at the same time to improve work efficiency.

The dyeing tank 110, the counter-dyeing tank 120, the dye accelerating tank 130, the cleaning tank 140, and the second cleaning tank 150 can be separated, that is, all or part of them are independently set up and moved (if they have a moving function). In addition, as shown in Figures 9 and 10, these trough bodies can also be fixedly assembled into a whole, forming an integral structure, and can be moved integrally.

10, in this embodiment, the pickup assembly 210 can be driven by the first drive assembly 220 (including the X-axis motor 221, the Y-axis motor 222, and the Z-axis motor 223) to move in the XYZ three-axis directions , So as to realize the movement, insertion, and pick-up of the slide 500. Wherein, the width direction of the glass slide 500 inserted into the accommodating groove 100 (that is, the width direction of the insertion area) is the X direction, and the arrangement direction of the glass slide 500 (that is, the thickness direction of the insertion area) inserted into the accommodating groove 100 is the Y direction, The insertion direction of the slide glass 100, that is, the vertical direction is the Z direction.

Further, in an embodiment, the transmission mechanism 200 includes a Y-axis rail 2221 and a Y-axis slider 2222 in the Y direction, an X-axis rail 2211 and an X-axis slider 2212 in the X direction, a Y-axis motor 222 and an X-axis motor 221. The X-axis motor 221 and the Y-axis motor 222 may be, for example, stepping motors or servo motors.

The Y-axis rail 2221 linearly extends in the Y direction and is fixed to the lower surface of the support member 230. The supporting member 230 may be a top wall part of the cabinet of the sample staining device or a supporting beam member or the like. The Y-axis slider 2222 is installed on the lower surface side of the Y-axis rail 2221 and can move along the Y-axis rail 2221. The Y-axis motor 222 moves the Y-axis slider 2222 in the Y direction through a corresponding transmission mechanism. For the transmission mechanism, for example, a belt-pulley mechanism or a rack and pinion mechanism can be used.

The X-axis rail 2211 extends linearly in the X direction and is fixed to the lower surface of the Y-axis slider 2222. The X-axis slider 2212 is installed on the lower surface side of the X-axis rail 2211 and can move along the X-axis rail 2211. The X-axis motor 221 moves the X-axis slider 2212 in the X direction through a corresponding transmission mechanism.

A pair of Y-axis slider 2222, X-axis rail 2211, X-axis slider 2212, X-axis motor 221, and Y-axis motor 222 are each provided. A pair of X-axis sliders 2212 has a pickup assembly 210 mounted on the lower side. In this way, the pickup assembly 210 can independently move in the X direction along the respective X-axis rail 2211. In addition, the pickup assembly 210 can also move independently in the Y direction along the general Y-axis rail 2221.

Further, in an embodiment, the first driving assembly 220 includes a Z-axis motor 223 and a transmission mechanism 2231 for the pickup assembly 210 (for example, the clamping member 2232). The Z-axis motor 223 lifts and lowers the clamping member 2232 through the transmission mechanism 2231. As the transmission mechanism 2231, for example, a belt pulley mechanism or a rack and pinion mechanism may be used.

In addition, some embodiments may further include a drying tank 160 and a blowing unit 610. The air blowing unit 610 may generate an air flow for drying, and blow the glass slide 500 in the drying tank 160, thereby achieving drying of the glass slide 500. The air blowing unit 610 includes, for example, an electric fan, which can forcibly send air into the air passage inside the drying tank 160. The slide glass 500 is placed on the second mounting part 161 inside the drying tank 160, and the air blowing unit 610 can blow air to the slide glass 500 continuously for a certain period of time to dry the slide glass 500. By providing the drying tank 160 and the air blowing unit 610, the stained glass slide 500 can be quickly dried, so that the time required for the staining process can be shortened. In addition, in the same way, the slide glass 500 can be inserted into and withdrawn from the second mounting member 51 by the transport mechanism 200, so the structure of the apparatus can be simplified.

In an embodiment, there is also a heater 620 for heating the air sent by the air blowing unit 610. The heater 620 is located between the air blowing unit 610 and the drying tank 160. The air sent by the air blowing unit 610 is heated when passing through the heater 620 and then sent into the air passage in the drying tank 160 in a state of warm air with a rising temperature. In this way, the slide glass 500 after the dyeing process can be dried more quickly, and therefore the time required for the dyeing process can be further shortened.

During the entire dyeing process, the first dye solution and the second dye solution will not fail in a short time, and the first dye solution and the second dye solution are used repeatedly. Considering the volatility of the first dye solution, please refer to Figure 10. In one embodiment, the first dye solution circuit control system is also included (not drawn in detail in the figure, but is only schematically represented by corresponding lines. The following related The liquid path control system is represented in this way) and the first dye solution sealed container 710. The first dye solution liquid path control system communicates with the first dye solution sealed container 710 and the dyeing tank 110 respectively to connect the dyeing tank 110 The corresponding first dye solution is recovered to the first dye solution sealed container 710, and the first dye solution in the first dye solution sealed container 710 is discharged into the dyeing tank 110 again.

Wherein, the first dye solution liquid path control system may include a first dye solution recovery system and a first dye solution inlet system. The first dye solution recovery system communicates with the first dye solution sealed container 710 and the dye tank 110, respectively. The corresponding first dye solution in the dyeing tank 110 is recovered to the first dye solution sealed container 710. The first dye liquor inlet system is respectively communicated with the first dye liquor sealed container 710 and the dyeing tank 110 to discharge the first dye liquor in the first dye liquor sealed container 710 into the dyeing tank 110 again.

In the above embodiments, the first dye liquor recovery system and the first dye liquor inlet system are used to realize recovery and liquor inlet control respectively. In contrast, in other embodiments, the first dye solution liquid path control system may also be an integrated system, which not only has a recovery function, but also has a liquid inlet function.

In an embodiment, the first dye solution recovery system includes a first dye solution recovery pipeline and a first dye solution recovery pressure source, and the first dye solution recovery pipeline is respectively connected to the dyeing tank 110 and the first dye solution sealed container 710 , The first dye solution recovery pressure source can be directly or indirectly connected with the first dye solution recovery pipeline. The first dye solution recovery pressure source sucks the first dye solution in the dyeing tank 110 into the first dye solution sealed container 710.

In one embodiment, the first dye liquor inlet system includes a first dye liquor inlet pipe and a first dye liquor inlet pressure source. The first dye liquor inlet pipe is connected to the dyeing tank 110 and the first dye liquor respectively. The sealed container 710 is connected, and the first dye liquor inlet pressure source can be directly or indirectly connected with the first dye liquor inlet pipe. The first dye liquor inlet pressure source discharges the first dye liquor in the first dye liquor sealed container 710 into the dyeing tank 110.

More commonly, both the first dye liquor recovery pressure source and the first dye liquor inlet pressure source can use air pressure to drive the liquid flow direction. Of course, other pressure methods are also feasible. In an embodiment, the first dye solution recovery pipeline and the first dye solution inlet pipeline are the same pipeline, and/or the first dye solution inlet pressure source and the first dye solution recovery pressure source are the same pressure source . For example, the first dye liquor recovery pipeline and the first dye liquor inlet pipeline are the same pipeline, and the recovery and liquid inlet functions are realized by connecting with different pressure sources and cooperating with the design of each pipeline.

Regarding the timing of recovery and feeding, in one embodiment, when the control unit determines that there is no sample to be dyed or receives the first dye solution recovery instruction issued by the user, the first dye solution liquid path control system sets the dyeing tank 110 The corresponding first dye solution inside is recovered to the first dye solution sealed container 710.

When the control unit determines that there is a sample that needs to be dyed or receives the first dye liquor input instruction from the user, the first dye liquor path control system discharges the first dye liquor in the first dye liquor sealed container 710 to In the dyeing tank 110.

Among them, since the first dye solution can be reused, the first dye solution sealed container 710 for storing the first dye solution does not need to be too large, and only needs to basically meet the amount of each dyeing used at the least. At this time, the first dye solution sealed container 710 can be a reagent bottle with a smaller volume, and there is no need to set a larger recovery bucket or other container. The reagent bottle can even be the original reagent bottle of the first dye solution (if there is an original reagent bottle in the first dye solution), so that when the first dye solution needs to be replaced, the reagent bottle can be directly replaced without any further discharge operation. When this reagent bottle is used as the first dye solution sealed container 710, its volume is small. In one embodiment, the reagent bottle can be set inside the smear preparation equipment, which can not only seal the first dye solution container 710. In order to protect, at the same time, because the reagent bottle is small, the compactness of the entire device can still be ensured. However, the use of larger recycling bins and other structures is not conducive to placing them in the equipment and will increase the volume of the equipment.

Since the first dye solution usually uses high-purity methanol as a solvent, it has a very strong volatility. If it is exposed to the air, it will quickly evaporate. The above structure can recycle and seal the first dye solution when not in use to avoid volatilization and prolong its service life.

Of course, in addition to being recycled to the first dye solution sealed container 710 for sealed storage when not in use, it also includes other methods.

In one embodiment, it further includes a first dyeing liquid cover, which is used to cover the corresponding opening on the dyeing tank 110 when the dyeing tank 110 is not in use, and to reopen the dyeing tank 110 when needed.

The first dye solution cover can be controlled separately or together with the cover of other containing tanks (for example, the counter-dye tank 120). When not in use, the first dye solution cover can close the dyeing tank 110 to avoid volatilization of the first dye solution.

The above two methods are only an example of the sealed preservation of the first dye solution, and the two methods can be selected and used according to the actual structure and requirements. The two methods can not only reduce the volatilization of the first dye solution, but also prevent some sundries in the equipment from falling into the first dye solution and avoid contamination of the first dye solution.

Regarding the timing of recovery or capping, in one embodiment, the method for the control unit to determine that no sample needs to be stained includes but is not limited to: no sample in the staining tank 110 and no sample to be stained is sent, and/or the staining tank 110 The idle time exceeds the set value.

Further, for the first dye solution of the present application, since it can be used repeatedly, its replacement frequency is greatly reduced, without frequent replacement, which can reduce the labor intensity of the operator. In the simplest way, the user can manually pick and place the first dye solution when it needs to be replaced.

Of course, in order to improve work efficiency and further realize automation, in one embodiment, a first dye liquor discharge system is further included. The first dye liquor discharge system is in communication with the dyeing tank 110 or the first dye liquor sealed container 710. When the control unit determines When the first dye liquor reaches the discharge requirement or after receiving the first dye liquor discharge instruction issued by the user, the first dye liquor discharge system discharges the first dye liquor in the dyeing tank 110 or the first dye liquor sealed container 710.

The discharge requirements of the first dye solution can be set according to actual needs. In one embodiment, the discharge requirements include, but are not limited to: the ratio of the number of times the sample is dyed to the volume of the first dye solution reaches 2 times/ml or more And/or the use time of the first dye solution reaches more than 2 days. For example, when 50 ml of the first dye solution is stored in the dyeing tank 110, the first dye solution can be discharged after the sample is dyed in the first dye solution for more than 100 times. The number of staining times is not absolutely equal to the number of stained samples. When the sample completes a complete staining action in the first staining solution, it is a staining. Due to different staining requirements or staining effects, the same sample may be in the first staining solution Perform multiple staining. When the first dye solution meets the above requirements, the first dye solution can be discharged and replaced manually or automatically by the user. Since the dye liquor is usually costly, recycling and reusing it can greatly reduce the cost.

Similar to the reuse of the first dye liquor, in one embodiment, it further includes a second dye liquor path control system and a second dye liquor recovery container 720, the second dye liquor path control system and the second dye liquor sealed container It communicates with the counter-dye tank 120 to recover the corresponding second dye liquor in the counter-dye tank 120 to the second dye liquor recovery container 720, and discharge the second dye liquor in the second dye liquor recovery container 720 to the re-dye again Inside the dye tank 120.

Similarly, the second dye solution liquid path control system may include a second dye solution recovery system and a second dye solution inlet system. The second dye solution recovery system is in communication with the second dye solution recovery container 720 and the counter dye tank 120. It is used to recover the corresponding second dye solution in the counter dye tank 120 to the second dye solution recovery container 720; the second dye solution inlet system communicates with the second dye solution recovery container 720 and the counter dye tank 120 to The second dye solution in the second dye solution recovery container 720 is discharged into the counter dye tank 120 again.

Of course, in some embodiments, the second dye liquor liquid path control system may also be an integral system, which not only has a recovery function, but also has a liquid inlet function.

In one embodiment, the second dye solution recovery system includes a second dye solution recovery pipeline and a second dye solution recovery pressure source, and the second dye solution recovery pipeline is respectively connected to the counter dye tank 120 and the second dye solution recovery container 720. Connected, the second dye solution recovery pressure source is directly or indirectly connected with the second dye solution recovery pipeline. The second dye solution recovery pressure source sucks the second dye solution in the counter dyeing tank 120 into the second dye solution recovery container 720.

The second dye liquor inlet system includes a second dye liquor inlet pipe and a second dye liquor inlet pressure source. The second dye liquor inlet pipe is respectively connected with the counter dye tank 120 and the second dye liquor recovery container 720, The second dye liquor inlet pressure source discharges the second dye liquor in the second dye liquor recovery container 720 into the counter dyeing tank 120.

The second dye liquor recovery pressure source and the second dye liquor inlet pressure source can refer to the settings of the first dye liquor recovery pressure source and the first dye liquor inlet pressure source. Among them, in one embodiment, the second dye liquor recovery pipeline and the second dye liquor inlet pipeline are the same pipeline, and/or the second dye liquor inlet pressure source and the second dye liquor recovery pressure source are The same source of stress.

The timing of the recovery and feeding of the second dye liquor may include but is not limited to the following methods:

When the control unit determines that there is no sample to perform counter-dyeing work or receives the second dye solution recovery instruction issued by the user, the second dye solution circuit control system recovers the corresponding second dye solution in the counter-dye tank 120 to the second dye solution. Liquid recovery container 720.

When the control unit determines that there is a sample that needs to be re-stained or receives the second dye liquor input instruction issued by the user, the second dye liquor path control system re-discharges the second dye liquor in the second dye liquor recovery container 720 To the counterstaining tank 120.

Similarly, in addition to recovering and sealing for storage, in one embodiment, a second dye solution cover may also be included. When the control unit determines that no sample needs to be counter-stained or receives a user’s instruction to cover the second dye solution , Control the second dye solution cover to cover the counter dye tank 120; when the control unit determines that there is a sample that needs to be counter dyed or receives an instruction to open the second dye solution from the user, it controls the second dye solution cover to open the counter dye tank 120.

Among them, in one embodiment, the method for the control unit to determine that no sample needs to be counterstained includes but is not limited to: there is no sample in the counterstaining tank 120 and the dyeing tank 110 and no sample to be sent, and/or the counterstaining tank 120 idle time exceeds the set value.

Similarly, for the dye accelerating tank 130, the cleaning tank, and the second cleaning tank 150, the recovery and inflow of the liquid in them can also be realized by referring to the aforementioned recovery and liquid inlet system.

The recovery and liquid inlet systems corresponding to the dye accelerating tank 130, the cleaning tank 140, and the second cleaning tank 150 can be implemented by using the same structure as the first dye liquid path control system and the first dye liquid sealed container 710 described above. For example, the dye accelerating tank 130 communicates with the dye accelerating liquid recovery container 730 through the accelerating liquid liquid path control system to realize recovery and liquid inflow. The cleaning tank 140 is in communication with the cleaning liquid recovery container 740 through the cleaning liquid liquid path control system, so as to realize the recovery and liquid inflow. The second cleaning tank 150 is in communication with the second cleaning liquid recovery container 750 through the second cleaning liquid liquid path control system, so as to realize recovery and liquid inlet.

Of course, a corresponding cover can also be used to seal the dye accelerating tank 130, the cleaning tank 140, and the second cleaning tank 150.

In one embodiment, it further includes a dye-promoting liquid cover. When the control unit determines that no sample needs to perform dye-promoting work or receives an instruction from the user to cover the dye-promoting liquid, it controls the dye-promoting liquid cover to cover the corresponding dye-promoting tank 130 , And reopen the dye accelerating tank 130 when needed.

In an embodiment, it further includes a cleaning liquid cover. When the control unit determines that no sample needs to be cleaned before counter-dyeing or receives a capping cleaning liquid instruction from the user, the cleaning liquid cover is controlled to cover the corresponding opening on the cleaning tank 140 , And reopen the cleaning tank 140 when needed.

In an embodiment, the second cleaning tank 150 may also be provided with a corresponding second cleaning tank cover, and the second cleaning tank 150 is sealed under the control of the control unit.

One or more of them can be selected for use according to the actual needs of the recovery and inlet system corresponding to the above-mentioned holding tanks, and the corresponding cover. Generally speaking, the first dye liquor and the second dye liquor will be recycled first to reduce Dyeing costs.

The liquid inlet system and recovery system corresponding to the above dyeing tank 110, counter-dyeing tank 120, dye accelerating tank 130, cleaning tank 140, and second cleaning tank 150 can be used alternatively, or both can be used at the same time. The recovery pressure source and the liquid inlet pressure source can be set separately, or the same pressure source can be used as both suction and liquid inlet pressure sources.

In the case of the above-mentioned recovery system, each liquid can be recovered and stored after the dyeing work is completed every day (or each batch of samples), and will be released into the corresponding holding tank 100 when it is to be used next time. Of course, after a period of continuous use, each liquid can be recovered and stored to reduce the volatilization of the reagent and avoid reagent contamination. The reagents in each containing tank 100 can be set to be replaced regularly or after staining a quantitative sample.

Further, in addition to providing an independent cover body, please refer to FIG. 9. In one embodiment, the sample staining device further includes an integral outer cover 300, which is used to cover the accommodating tank 100 when it is not in use. The corresponding opening on the accommodating groove 100. The non-use state may refer to a non-working state, that is, when staining is not performed, or when a glass slide is not placed in a certain holding tank 100. The integral outer cover 300 can uniformly cover all the accommodating grooves 100, for example, when a glass slide is not placed in a certain accommodating groove 100, or, at the time indicated for covering the corresponding accommodating groove, the integral outer cover 300 can The accommodating groove 100 is shielded.

Of course, the integral outer cover 300 can be manually covered on the accommodating slot 100 by a worker, and a transmission structure can also be designed to cover the accommodating slot 100 automatically.

In an embodiment, please refer to FIG. 9. The sample staining device further includes a mounting base 400 and a second driving assembly (not shown in the figure). The accommodating groove 100 is mounted on the mounting base 400, and the integral outer cover 300 and mounting Any one of the seats 400 is in transmission connection with the second drive assembly, and is used to move the integral outer cover 300 and the receiving groove 100 on the mounting seat 400 relative to each other under the driving of the second drive assembly, so as to shield the corresponding receiving grooves 100 Opening.

In one embodiment, the accommodating groove 100 moves so that the integral outer cover 300 can cover the accommodating groove 100. Wherein, each tank body in the accommodating tank 100 can be moved individually or as a whole.

In FIG. 9, the second driving assembly is used to drive the mounting base 400, and the overall outer cover 300 can be kept different, and it is located above the corresponding opening of each accommodating slot 100. The second driving component can drive the mounting base 400 to move along the x-axis. During operation, the integral outer cover 300 opens the corresponding opening of the containing groove 100. When the accommodating groove 100 needs to be covered, the second driving component drives the mounting seat 400 to communicate with the accommodating groove 100 to move to the integral outer cover 300 until the integral outer cover 300 covers the corresponding opening of the accommodating groove 100.

The sample dyeing device can be used to implement the steps of the above dyeing method. Of course, the above dyeing method is not limited to the device shown in this embodiment.

On the other hand, the present application also provides a dyeing solution combination for sample dyeing, which includes a first dyeing solution and a second dyeing solution. The first dyeing solution is a mixture of the first biological dye and the second biological dye. One biological dye can stain acidophilic substances in the sample, and the second biological dye can stain alkaliphilic substances in the sample. The second dye solution is a mixture of the third biological dye and the buffer solution, that is, most of the effective components in the second dye solution are the buffer solution and the third biological dye. The third biological dye can dye alkaliphilic substances or acidophilic substances in the sample, and it can be the same as or different from the first biological dye or the second biological dye. The first biological dye, the second biological dye and the third biological dye can be selected from the dyes described above.

In some embodiments, the second dye solution may be a mixture of the first biological dye, the third biological dye and the buffer. For example, the Giemsa dye solution consists of azure (formed by oxidation of methylene blue) as the third biological dye, and eosin as the first biological dye, but the amount of azure is significantly greater than the amount of eosin. At this time, the volume ratio of the first biological dye is much smaller than that of the third biological dye. For example, the ratio of the first biological dye to the third biological dye may be less than 1/3.

The staining solution combination can be used in but not limited to the above-mentioned staining method and sample staining device, and it can also be used in other staining methods and sample staining devices.

As a more specific embodiment, the first biological dye is eosin dye, the second biological dye is methylene blue dye, and the third biological dye is azure dye. Of course, the first biological dye, the second biological dye and the third biological dye can also be replaced with other dyes capable of dyeing acidophilic substances and alkaliphilic substances, respectively.

Further, in order to meet the usage habits of most clinical departments, in one embodiment, the first dye solution is Wright-Giemsa dye, and the Wright-Giemsa dye includes eosin dye and methylene blue dye. The second dye solution is a mixture of Giemsa dye and buffer, and the Giemsa dye contains methylene blue dye as the third biological dye.

Wherein, the second dye solution can also be replaced with a mixture of other dye solution with methylene blue dye and buffer solution, such as Liu's B dye solution.

Further, in order to improve the dyeing effect in the counterstaining step S30, in the second dyeing solution, the volume ratio a of the Giemsa dye or Liu's B dye and the buffer is set to a value of 1/30≤a≤1/10.

Preferably, in an embodiment, the volume ratio a of the Giemsa stain or Liu's B stain and the buffer in the second stain is 1/25.

The above uses specific examples to illustrate the application, which are only used to help understand the application and not to limit the application. For those of ordinary skill in the art, according to the idea of the present application, the above-mentioned specific embodiments can be changed.

Claims (56)

A sample staining method, characterized in that it comprises: Selection steps: select the slides coated with samples; Dyeing step: soak the sample in a dyeing tank and then take it out. The dyeing tank stores a first dye solution. The first dye solution is a mixture of a first biological dye and a second biological dye. The first biological dye can Dyeing the acidophilic substance in the sample, and the second biological dye can dye the alkaliphilic substance in the sample; Counter-staining step: soak the sample in a counter-staining tank and take it out. The counter-staining tank stores a second dye solution. The second dye solution is different from the first dye solution. The second dye solution is the second dye solution. A mixture of three biological dyes and buffers, where the third biological dye can stain basophils or acidophils in the sample; The method further includes: selecting the next glass slide coated with the sample, and repeating the staining step to the counter staining step. 3. The sample staining method according to claim 1, further comprising a stain accelerating step: immersing the sample processed in the staining step in a stain accelerating tank and then taking it out, and the stain accelerating tank stores a buffer solution; The dye accelerating step is located after the dyeing step and before the counterstaining step. 3. The method for staining a sample according to claim 2, wherein in the step of accelerating staining, the sample is soaked in the buffer for 1-5 minutes. The sample staining method according to claim 2 or 3, further comprising the step of cleaning before counterstaining: soaking the sample processed in the dye accelerating step in a cleaning tank and then taking it out, the cleaning tank stores Clean liquid. 5. The sample staining method according to claim 1, further comprising a cleaning step before counterstaining: cleaning the sample with a cleaning liquid, the cleaning liquid being stored in a cleaning tank; The washing step before counterstaining is located after the dyeing step and before the counterstaining step. The sample staining method according to claim 4 or 5, wherein in the pre-counter-staining washing step, the sample washing time is 1-5 minutes. The sample staining method according to any one of claims 1-6, wherein the second dye solution further comprises a first biological dye, the third biological dye is a basic biological dye, and the first The volume ratio of the biological dye is much smaller than the volume ratio of the third biological dye. 7. The sample staining method according to any one of claims 1-7, wherein the first biological dye is eosin dye, the second biological dye is methylene blue dye, and the third biological dye is azure dye. The sample staining method according to any one of claims 1-6, wherein the first dye solution is Wright-Giemsa stain, and the second dye solution is a combination of Giemsa stain and buffer The mixture, or the second dye solution is a mixture of Liu's B dye solution and buffer. The sample staining method of claim 9, wherein in the second staining solution, the volume ratio a of the Giemsa stain or Liu's B stain to the buffer solution is 1/30≤ a≤1/10. The sample staining method according to any one of claims 1-10, wherein in the counterstaining step, the soaking time of the sample is 5-15 minutes. 11. The sample staining method according to any one of claims 1-11, wherein in the staining step, the soaking time of the sample is 1-5 minutes. The sample staining method according to any one of claims 1-12, further comprising a second cleaning step: cleaning the sample after the counterstaining step. The method for staining a sample according to any one of claims 1-13, further comprising: When the control unit determines that there is no sample to perform the dyeing step or receives the first dye solution recovery instruction issued by the user, it recovers the first dye solution in the dyeing tank to the first dye solution sealed container for storage, and waits. One-time use When the control unit determines that there is a sample that needs to perform the dyeing step or receives the first dye liquor input instruction issued by the user, the first dye liquor stored in the first dye liquor sealed container is re-sent to the dyeing tank, To reuse the first dye solution. The sample staining method according to any one of claims 1-14, further comprising: When the control unit determines that there is no sample to perform the dyeing step or receives an instruction to cover the first dye solution issued by the user, the control unit covers the first dye solution in the dyeing tank and waits for the next use; When the control unit determines that there is a sample that needs to perform the dyeing step or receives a user's instruction to open the first dye solution, it uncovers the dyeing tank to reuse the first dye solution. The method for staining a sample according to claim 14 or 15, wherein the method for the control unit to determine that no sample needs to be subjected to the staining step comprises: no sample in the staining tank and no sample waiting to be sent, and/ Or the idle time of the dyeing tank exceeds the set value. The sample staining method according to any one of claims 1-16, wherein when the control unit determines that the first dye solution meets the set discharge requirement or receives the first dye solution discharge instruction issued by the user, The first dye liquor is discharged. The sample staining method according to claim 17, wherein the discharge requirements include that the number of staining times per milliliter of the first dye solution is greater than or equal to 2 and/or the use time of the first dye solution reaches 2 days the above. 18. The sample staining method according to any one of claims 1-18, further comprising: When the control unit determines that there is no sample to perform the counterstaining step or receives the second dyeing solution recovery instruction issued by the user, the second dyeing solution in the counterstaining tank is recovered to the second dyeing solution recovery container for storage, Waiting for the next use; When the control unit determines that there is a sample that needs to be subjected to the counter-staining step or receives a second dye solution input instruction issued by the user, the second dye solution stored in the second dye solution recovery container is re-sent to the counter dye Tank to reuse the second dye solution. The sample staining method according to any one of claims 1-19, further comprising: When the control unit determines that there is no sample to perform the counterstaining step or receives an instruction from the user to cover the second dyeing solution, cover the second dyeing solution in the counterstaining tank and wait for the next use; When the control unit determines that there is a sample that needs to be subjected to the counter-staining step or receives an instruction to open the second dye solution from the user, the cover of the counter-dye tank is released to reuse the second dye solution. The sample staining method according to claim 19 or 20, wherein the method for the control unit to determine that no sample needs to be subjected to the counterstaining step includes: there is no sample in the counterstaining tank and the staining tank and no sample to be prepared. Feeding, and/or the idle time of the counterstaining tank exceeds the set value. The sample staining method according to any one of claims 1-21, wherein when the control unit determines that the second dye solution meets the set discharge requirement or receives the second dye solution discharge instruction issued by the user, The second dye liquor is discharged. The sample staining method according to claim 4 or 5, further comprising: When the control unit determines that there is no sample to perform the pre-counter-dyeing cleaning step or receives the cap cleaning liquid instruction from the user, cap the cleaning liquid in the cleaning tank and wait for the next use; When the control unit determines that there is a sample that needs to perform the pre-counter-staining cleaning step or receives an instruction to open the cleaning liquid from the user, the cleaning tank is uncovered to reuse the cleaning liquid. 3. The sample staining method of claim 2, further comprising: When the control unit determines that there is no sample to perform the dye accelerating step or receives an instruction to cap the dye accelerating solution from the user, cap the buffer in the dye accelerating tank and wait for the next use; When the control unit determines that there is a sample that needs to perform the staining step or receives an instruction to open the staining solution issued by the user, the control unit uncovers the staining tank to reuse the buffer solution. A smear preparation equipment, which is characterized by comprising a containing tank, a transmission mechanism and a control unit, the containing tank comprising: A dyeing tank for storing a first dye solution, the first dye solution is a mixture of a first biological dye and a second biological dye, the first biological dye can dye acidophilic substances in the sample, and the second The biological dye can dye the alkaliphilic substance in the sample, and the dyeing tank has a first opening through which the glass slide coated with the sample can pass; A counterstaining tank for storing a second dye solution that is different from the first dye solution. The second dye solution is a mixture of a third biological dye and a buffer solution. Basophils are dyed, and the counterstaining tank has a second opening through which the slide can pass; The transfer mechanism is used for picking up the glass slide and driving the glass slide to move; The control unit outputs a control signal to control the conveying mechanism to drive the sample to be immersed in the dyeing tank for dyeing, and then immersed in the counter dyeing tank for counter dyeing. The smear preparation equipment according to claim 25, further comprising a first dye solution liquid path control system and a first dye solution sealed container, the first dye solution liquid path control system and the first dye solution respectively The sealed container communicates with the dyeing tank, and is used to recover the corresponding first dye solution in the dyeing tank to the first dye solution sealed container, and to discharge the first dye solution in the first dye solution sealed container to the dyeing tank again . The smear preparation equipment of claim 26, wherein the first dye solution liquid path control system comprises a first dye solution recovery system and a first dye solution inlet system, and the first dye solution recovery system They are respectively communicated with the first dye solution sealed container and the dyeing tank to recover the corresponding first dye solution in the dyeing tank to the first dye solution sealed container; the first dye solution inlet system is respectively sealed with the first dye solution The container is communicated with the dyeing tank, and is used to discharge the first dye solution in the first dye solution sealed container into the dyeing tank again. The smear preparation equipment according to claim 27, wherein the first dye solution recovery system comprises a first dye solution recovery pipeline and a first dye solution recovery pressure source, and the first dye solution recovery pipeline Respectively communicating with the dyeing tank and the first dye solution sealed container, and the first dye solution recovery pressure source sucks the first dye solution in the dyeing tank into the first dye solution sealed container; The first dye liquor inlet system includes a first dye liquor inlet pipe and a first dye liquor inlet pressure source, and the first dye liquor inlet pipe is respectively connected with the dyeing tank and the first dye liquor sealed container , The first dye liquor inlet pressure source discharges the first dye liquor in the sealed container of the first dye liquor into the dyeing tank. The smear preparation equipment according to claim 28, wherein the first dye solution recovery pipeline and the first dye solution inlet pipeline are the same pipeline, and/or the first dye solution The inlet pressure source and the first dye solution recovery pressure source are the same pressure source. The smear preparation equipment according to any one of claims 26-29, wherein when the control unit determines that no sample needs to be dyed or receives the first dye solution recovery instruction issued by the user, the first dye solution A dye solution liquid path control system recovers the corresponding first dye solution in the dyeing tank to the first dye solution sealed container; when the control unit determines that a sample needs to be dyed or receives the first dye solution inflow from the user After the instruction, the first dye liquor path control system discharges the first dye liquor in the sealed container of the first dye liquor into the dyeing tank again. The smear preparation device according to claim 30, wherein the first dye solution sealed container is a reagent bottle, and the reagent bottle is arranged inside the smear preparation device. The smear preparation device according to any one of claims 25-31, further comprising a first dye solution cover, when the control unit determines that there is no sample to be dyed or receives a cover first issued by the user After a dyeing solution instruction, the first dyeing solution cover covers the corresponding opening on the dyeing tank, and reopening the dyeing tank when needed. The smear preparation equipment according to any one of claims 30-32, wherein the method for the control unit to determine that no sample needs to be stained includes: no sample in the staining tank and no sample to be stained , And/or the idle time of the dyeing tank exceeds the set value. The smear preparation equipment of claim 26, further comprising a first dye solution discharge system, the first dye solution discharge system is in communication with the dye tank or the first dye solution sealed container, when the control unit determines When the first dye liquor reaches the discharge requirement or after receiving the first dye liquor discharge instruction issued by the user, the first dye liquor discharge system discharges the first dye liquor in the dyeing tank or the first dye liquor sealed container. The smear preparation equipment according to claim 34, wherein the discharge requirements include: dyeing at least twice per milliliter of the first dye solution and/or the first dye solution is used for more than 2 days . The smear preparation equipment according to claim 25, further comprising a second dye liquor path control system and a second dye liquor recovery container, the second dye liquor path control system and the second dye liquor sealed The container communicates with the counter-dyeing tank, and is used to recover the corresponding second dye solution in the counter-dye tank to the second dye solution recovery container, and to discharge the second dye solution in the second dye solution recovery container to the counter dye again In the slot. The smear preparation equipment of claim 36, wherein the second dye solution liquid path control system comprises a second dye solution recovery system and a second dye solution inlet system, and the second dye solution recovery system It communicates with the second dye liquor recovery container and the counter dye tank to recover the corresponding second dye liquor in the counter dye tank to the second dye liquor recovery container; the second dye liquor inlet system and the second dye liquor recovery The container communicates with the counter-dyeing tank, and is used for re-discharging the second dye liquor in the second dye-liquor recovery container into the counter-dyeing tank. The smear preparation equipment according to claim 37, wherein the second dye solution recovery system comprises a second dye solution recovery pipeline and a second dye solution recovery pressure source, and the second dye solution recovery pipeline Respectively connected with the counter-dye tank and the second dye-liquor recovery container, and the second dye-liquid recovery pressure source sucks the second dye liquid in the counter-dye tank into the second dye-liquor recovery container; The second dye liquor inlet system includes a second dye liquor inlet pipe and a second dye liquor inlet pressure source, and the second dye liquor inlet pipe is respectively connected to the counter dye tank and the second dye liquor recovery container Connected, the second dye liquor inlet pressure source discharges the second dye liquor in the second dye liquor recovery container into the counter-dye tank. The smear preparation equipment according to claim 38, wherein the second dye solution recovery pipeline and the second dye solution inlet pipeline are the same pipeline, and/or the second dye solution The inlet pressure source and the second dye liquor recovery pressure source are the same pressure source. The smear preparation equipment according to any one of claims 36-39, wherein when the control unit determines that no sample needs to be counter-stained or receives a second dye solution recovery instruction issued by the user, the The second dye liquor path control system recovers the corresponding second dye liquor in the counter dye tank to the second dye liquor recovery container; When the control unit determines that there is a sample that needs to be re-stained or receives a second dye liquor input instruction issued by the user, the second dye liquor path control system recovers the second dye liquor in the container The second dye solution is discharged into the counter dye tank again. The smear preparation device according to any one of claims 25-40, further comprising a second dye solution cover, when the control unit determines that there is no sample to be counter-dyeing work or receives a second cover issued by the user After the dye solution instruction, the second dye solution cover is controlled to cover the counter dye tank; when the control unit determines that there is a sample that needs to be counter dyed or receives an instruction to open the second dye solution from the user, it controls the second dye solution The dye solution cover opens the counter-dye tank. The smear preparation equipment according to any one of claims 25-41, wherein the accommodating tank further comprises a dye accelerating tank for storing a buffer solution, and the dye accelerating tank has a channel for the glass slide to pass through. The third opening; the control unit outputs a control signal to control the conveying mechanism to drive the sample to sequentially move to the dyeing tank for soaking for dyeing, the dyeing tank for accelerating dyeing and the counter-dyeing tank Soak for counterstaining. The smear preparation equipment according to claim 42, wherein the containing tank further comprises a cleaning tank for storing cleaning liquid, and the cleaning tank has a fourth opening for the glass slide to pass through; After the sample is finished in the dye accelerating tank and before the counter dye in the counter dye tank, the control unit outputs a control signal to control the conveying mechanism to drive the sample to move into the cleaning tank for cleaning. The smear preparation equipment according to claim 43, wherein the dyeing tank, the dye accelerating tank, the cleaning tank and the counter dyeing tank are arranged in a row in sequence. The smear preparation equipment according to any one of claims 25-41, wherein the accommodating tank further comprises a cleaning tank for storing cleaning liquid, and the cleaning tank has a first through which the glass slide can pass. Four openings; the control unit outputs a control signal, and controls the conveying mechanism to drive the sample to sequentially move to the dyeing tank for soaking for dyeing, cleaning in the washing tank and soaking in the counterstaining tank for counterstaining . The smear preparation device according to claim 43 or 45, further comprising a cleaning liquid cover, when the control unit determines that no sample needs to be cleaned before counterstaining or after receiving a cover cleaning liquid instruction from the user, The cleaning liquid cover is controlled to cover the corresponding opening on the cleaning tank, and the cleaning tank is reopened when needed. The smear preparation device according to claim 42, further comprising a dye-promoting liquid cover, when the control unit determines that no sample needs to be used for dye-promoting work or receives an instruction from the user to cover the dye-promoting liquid, the control unit The dye-promoting liquid cover covers the corresponding opening on the dye-promoting tank, and reopens the dye-promoting tank when needed. The smear preparation equipment according to any one of claims 25-47, further comprising a mounting seat and a first drive assembly, the accommodating groove is mounted on the mounting seat, and the mounting seat is connected to the first drive assembly. A drive assembly is connected in transmission, and the accommodation slot on the mounting seat moves under the drive of the first drive assembly. The smear preparation equipment according to claim 25, further comprising an integral outer cover, the integral outer cover being used for shielding the corresponding opening on the containing groove when the containing groove is not in use. The smear preparation equipment of claim 49, further comprising a mounting base and a first drive assembly, the containing groove is mounted on the mounting base, and any one of the integral outer cover and the mounting base is The first drive assembly is in a transmission connection, and is used for relative movement of the integral outer cover and the containing grooves on the mounting seat under the driving of the first drive assembly, so as to shield the corresponding openings on each containing groove. The smear preparation equipment according to any one of claims 25-50, further comprising a liquid inlet system, the liquid outlet of the liquid inlet system is in communication with the containing tank for injecting into the containing tank Corresponds to liquid. A dyeing solution combination for sample dyeing, which is characterized in that it comprises a first dyeing solution and a second dyeing solution. The first dyeing solution is a mixture of a first biological dye and a second biological dye. The dye can dye the acidophilic substance in the sample, the second biological dye can dye the alkaliphilic substance in the sample, the second dye solution is a mixture of the third biological dye and the buffer solution, the third biological dye It can stain basophils or acidophils in the sample. The dyeing solution combination of claim 52, wherein the first biological dye is eosin dye, the second biological dye is methylene blue dye, and the third biological dye is azure dye. The staining solution combination of claim 53, wherein the first staining solution is Wright-Giemsa stain, the second staining solution is a mixture of Giemsa stain and buffer, or the The second dye solution is a mixture of Liu's B dye solution and buffer. The staining solution combination according to any one of claims 52-54, wherein, in the second staining solution, the volume ratio a of the Giemsa stain or Liu's B staining solution and the buffer solution is a value It is 1/30≤a≤1/10. The dyeing solution combination of claim 52, wherein the second biological dye and the third biological dye are the same biological dye.

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