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WO2020185681A3 - Gold-mediated nucleic acid hybridization - Google Patents

Gold-mediated nucleic acid hybridization Download PDF

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Publication number
WO2020185681A3
WO2020185681A3 PCT/US2020/021716 US2020021716W WO2020185681A3 WO 2020185681 A3 WO2020185681 A3 WO 2020185681A3 US 2020021716 W US2020021716 W US 2020021716W WO 2020185681 A3 WO2020185681 A3 WO 2020185681A3
Authority
WO
WIPO (PCT)
Prior art keywords
gold
nucleic acid
aurous
acid hybridization
nucleobase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2020/021716
Other languages
French (fr)
Other versions
WO2020185681A2 (en
Inventor
Hosea M. NELSON
Sydnee GREEN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Publication of WO2020185681A2 publication Critical patent/WO2020185681A2/en
Publication of WO2020185681A3 publication Critical patent/WO2020185681A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H23/00—Compounds containing boron, silicon or a metal, e.g. chelates or vitamin B12
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/533—Physical structure partially self-complementary or closed having a mismatch or nick in at least one of the strands
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C12N2320/52—Methods for regulating/modulating their activity modulating the physical stability, e.g. GC-content

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)

Abstract

The present disclosure provides a gold-containing compound including an aurous gold atom and a nucleic acid strand that forms a toehold-stem-loop structure, in which the aurous gold atom is complexed with a mismatched pair of residues in the stem portion of the nucleic acid strand. The disclosure also provides a gold-containing double-stranded nucleic acid including an aurous gold coordinated between a first nucleobase and a mismatching second nucleobase. Related methods of increasing stability of a nucleic acid duplex, regulating an aurous-gold-catalyzed reaction, detecting an oligonucleotide, and detecting aurous gold in a substance are additionally provided.
PCT/US2020/021716 2019-03-09 2020-03-09 Compositions and methods related to gold-mediated nucleic acid hybridization Ceased WO2020185681A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962816103P 2019-03-09 2019-03-09
US62/816,103 2019-03-09

Publications (2)

Publication Number Publication Date
WO2020185681A2 WO2020185681A2 (en) 2020-09-17
WO2020185681A3 true WO2020185681A3 (en) 2020-10-22

Family

ID=72425921

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2020/021716 Ceased WO2020185681A2 (en) 2019-03-09 2020-03-09 Compositions and methods related to gold-mediated nucleic acid hybridization

Country Status (1)

Country Link
WO (1) WO2020185681A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025217587A1 (en) * 2024-04-12 2025-10-16 Biostate Ai, Incorporated Methods and systems for analyzing molecules based on nucleic acid displacement reactions

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8710199B2 (en) * 2010-06-23 2014-04-29 California Institute Of Technology Signal activated molecular delivery
WO2015116502A1 (en) * 2014-01-31 2015-08-06 The Regents Of The University Of California Detection and treatment of gnaq mutant uveal melanoma cells with gold nanoparticles
US9206419B2 (en) * 2011-06-23 2015-12-08 California Institute Of Technology Targeting domain and related signal activated molecular delivery

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8710199B2 (en) * 2010-06-23 2014-04-29 California Institute Of Technology Signal activated molecular delivery
US9206419B2 (en) * 2011-06-23 2015-12-08 California Institute Of Technology Targeting domain and related signal activated molecular delivery
WO2015116502A1 (en) * 2014-01-31 2015-08-06 The Regents Of The University Of California Detection and treatment of gnaq mutant uveal melanoma cells with gold nanoparticles

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ERIC ENNIFAR, PHILIPPE WALTER AND PHILIPPE DUMAS: "A crystallographic study of the binding of 13 metal ions to two related RNA duplexes", NUCLEIC ACIDS RESEARCH, vol. 31, no. 10, 2003, pages 2671 - 2682, XP055749214 *
MASOOD FEREIDOONNEZHAD, HAMID R. SHAHSAVARI, ELAHEH LOTFI, MOJGAN BABAGHASABHA, MOTAHAREH FAKHRI, ZEINAB FAGHIH, ZAHRA FAGHIH, M.: "Benzyl isocyanide) gold (I) pyrimidine-2- thiolate complex: Synthesis and biological activity", APPLIED ORGANOMETALLIC CHEMISTRY, vol. 32, no. 3, e4200, 2018, pages 1 - 7, XP055749287 *
SYDNEE A. GREEN, HAYDEN R. MONTGOMERY, TYLER R. BENTON, NEIL J. CHAN, AND HOSEA M. NELSON: "Regulating Transition-Metal Catalysis through Interference by Short RNAs", ANGEWANDTE CHEMIE, vol. 58, 16 July 2019 (2019-07-16), pages 16400 - 16404, XP055749223 *
WRAGG DARREN, ANDREIA DE ALMEIDA, RICCARDO BONSIGNORE , FRITZ E KÜHN , STEFANO LEONI, ANGELA CASINI: "On the Mechanism of Gold/NHC Compounds Binding to DNA G-Quadruplexes: Combined Metadynamics and Biophysical Methods", ANGEWANDTE CHEMIE, vol. 57, no. 44, 2018, pages 14524 - 14528, XP055749259 *

Also Published As

Publication number Publication date
WO2020185681A2 (en) 2020-09-17

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