WO2020179719A1 - Sweet potato plant and anthocyanin-based pigment composition derived from sweet potato - Google Patents

Abstract

The present invention addresses the problem of providing an anthocyanin-based pigment composition which is derived from a sweet potato, and which has color developing characteristics that exhibit a vivid red color tone and has excellent compound stability.　 The present invention pertains to a sweet potato plant which contains a large amount of a pelargonidin-based anthocyanin compound as a pigment component and has the characteristics described below: (1) the sweet potato plant belongs to a mating population of Kyukei 89360-8 and Kyukei 294, or a progeny population thereof; (2) as the composition of an anthocyanin-based pigment contained in a tuberous root, the ratio of the peak area of the pelargonidin-based anthocyanin compound to the peak areas of all the anthocyanin compounds in HPLC analysis is 70% or greater; and (3) as the composition of the anthocyanin-based pigment contained in the tuberous root, the ratio of the peak area of the anthocyanin compound having a hydroxycinnamic acid-modified group to the peak areas of all the anthocyanin compounds in the HPLC analysis is 50% or greater.

Description

Sweet potato plant and sweet potato-derived anthocyanin pigment composition

The present invention relates to a sweet potato plant containing a high content of a pelargonidin-based anthocyanin compound having a hydroxycinnamon acid modifying group as a pigment component. The present invention also relates to a sweet potato-derived anthocyanin-based pigment composition having bright red color development and compound stability.

Anthocyanin-based pigments are pigment compositions that are aggregates of a plurality of similar compounds, and the compound composition varies greatly depending on the type and variety of the raw material plant, the growing period, the growing environment, and the like. In addition to the combination of anthocyanidin, which is an aglycone, and a sugar molecule, anthocyanin dyes have a huge number of combinations of similar compounds depending on the presence or absence and type of organic acids and various modifying groups, and each has a different molecular tone and stability. It has the property.
Sweet potato pigment is a kind of anthocyanin pigment mainly produced from the root mass of sweet potato, and it is a pigment material that shows relatively good odor characteristics derived from raw materials and does not require excessive deodorization operation in the refining process. is there. In addition, since sweet potato pigment is a natural pigment derived from plant raw materials, it overlaps with the natural orientation of consumers in recent years, and as an excellent pigment material in many fields such as food and drink, cosmetics, quasi-drugs, and pharmaceuticals. It's being used.

Ordinary sweet potato-derived anthocyanin pigments have a composition mainly composed of cyanidin-based anthocyanins and peonidin-based anthocyanins in terms of the type of aglycone. As its color development characteristics, it exhibits a purple to magenta tone in a weakly acidic range reflecting the compound composition. Therefore, when a bright and bright red anthocyanin dye is desired, a normal sweet potato dye should be used. I can't.
Here, when an anthocyanin-based pigment exhibiting a bright and vivid red tone is desired, the sweet potato pigment having a reddish-purple color tone is not suitable, and therefore the red radish dye is considered to be suitable in terms of its color-developing characteristics. However, the red radish dye has an essential problem regarding odor characteristics and return odor derived from the characteristics of its glucosinolate composition.
Further, as a general problem of anthocyanin dyes, it has been pointed out that stable maintenance of color development characteristics of dye compounds has been pointed out. However, sweet potato dyes and red radish dyes have compositional characteristics containing a large amount of acylated anthocyanins. , It is recognized that the dye composition has relatively excellent stability. However, in the field of foods and drinks that are premised on light exposure in product distribution, there are some cases where the stability of ordinary sweet potato pigments and red radish pigments is not sufficient, and in particular, anthocyanins with even better light resistance. Development of dyes is required.

US Patent Application Publication No. 2015/0017303 International Publication WO2013 / 079518 (International application corresponding to the US application relating to Patent Document 1)

Mi Jin Lee et al., Journal of Agricultural and FoodChemistry 2013, 61(12) p3148-3158, Characterization and Quantitation of Anthocyanins in Purple-Fleshed Sweet Pottoes Cultivated in-D MS.

The present invention has been made in view of the above-mentioned circumstances of the prior art, and a subject matter thereof is a sweet potato-derived anthocyanin-based pigment composition having a color-developing property exhibiting a bright red color tone and excellent compound stability. The purpose is to provide.

As a result of intensive research conducted by the present inventors in the situation of the above-mentioned prior art, when two specific strains, which are the breeding strains produced by the present inventors, were combined as the original varieties and crossed, the mating population and the progeny were found. From the population, it was found that sweet potato plants containing a high content of pelargonidin-based anthocyanin compounds, which are not contained in plants of normal sweet potato varieties, as a main pigment component emerge.
Further, in the sweet potato plant, since a specific site of the sugar chain contained a high amount of an anthocyanin compound acylated and modified with hydroxycinnamon acids, a bright red color-developing anthocyanin pigment composition having excellent stability was obtained. It was recognized as a sweet potato plant.
Therefore, the present inventors prepared an anthocyanin-based pigment composition from the roots of the sweet potato plant, and found that the pigment composition exhibited a maximum absorption wavelength equivalent to that of a red dicon pigment and produced a bright red tone. It was shown that the sweet potato-derived pigment composition to be presented can be prepared. In addition, when a light resistance test was conducted by light irradiation, it was confirmed that the light stability was significantly superior to that of general sweet potato dyes and red radish dyes. In addition, it was also shown that the odor characteristics were as good as those of ordinary sweet potato pigments.

Here, the respective progeny strains derived from the parent strains "Kyushu 89360-8" and "Kyushu 294" are not pelargonidin-type anthocyanin-containing strains, but the cyanidin-type strains like the normal sweet potato strains. A line containing anthocyanins and paeonidine-type anthocyanins as pigment components. Surprisingly, a high content of pelargonidin-type anthocyanins is accumulated from the cross population of the "9-type 89360-8" and "9-type 294" crosses. Sweet potato plants appeared at a constant frequency.
Although the details of the mechanism of action of the production mechanism are unknown, in the mating group of the "9-strain 89360-8" and "9-strain 294", the sweet potato that acquired the metabolic function that enables the production and accumulation of pelargonidin. It is speculated that plants are likely to appear.
Furthermore, the produced sweet potato plant has a remarkably high proportion of R ₂ in the following structural formula (I) being a caffeic acid-modifying group that is a hydroxycinnamic acid, and has an enzymatic activity that controls caffeic acid acylation at the site. It was recognized as a sweet potato plant with a suitable genetic background.

Here, in the past large breeding studies of sweet potatoes, in view of the fact that there is no report of a line containing a high amount of pelargonidin anthocyanin having a modifying group of hydroxycinnamic acid in the sweet potato plant produced above, in view of the fact that there is no report. The phenotype relating to the pigment composition expressed by the sweet potato plant according to the present invention was recognized as a trait based on the characteristic genetic background that appears in the mated population obtained by crossing the above two original varieties. In addition, it was presumed that it would be difficult to obtain the sweet potato plant according to the present invention from the viewpoint of its genetic background from the sweet potato variety lines other than the above-mentioned original varieties.

In addition, the sweet potato plant according to the present invention has a genetic background in which a pelargonidin-based anthocyanin compound can be accumulated in a high content as a "major pigment".
Here, as sweet potato lines containing pelargonidin anthocyanins that have been reported so far, the “Borami” line in Korea (Non-Patent Document 1) and the “RSWP” line in Europe (Patent Document 1, etc.) have been reported. There is. However, these lines are sweet potato lines of a different origin from the sweet potato plant according to the present invention, and when the pigment composition is prepared with these lines, it is not possible to realize the coloring property exhibiting a bright red color tone.
Specifically, according to Non-Patent Document 1, the main component of the anthocyanin compound contained in the clump root of the "Borami" line is "peonidin-based anthocyanin", and the content of pelargonidin-based anthocyanin is 36% (Cy) in the total anthocyanin compound. :Pn:Pg=1:2.9:2.2) (Non-Patent Document 1, Table 1 and p. 3155). In this respect, it was recognized that the color development characteristics of the Borami dye composition are consistent with the fact that the color tone is a reddish purple color tone different from that of the red radish dye.
Further, the present applicant examined the compositional characteristics of the “RSWP” line described in Patent Document 1 (Examination Example 1 of the present specification), and found in paragraph 0084, which is an example of Patent Document 1, that the RSWP line was It was described that the pelargonidin-based anthocyanin content was only 58 mol% of the total anthocyanin compounds (a value less than 70% in terms of the content ratio of the total anthocyanin compounds: see Study Example 1 in the present specification). In this respect, the RSWP strain was recognized as a strain in which the pelargonidin-based anthocyanin content was not sufficiently high, and it was also recognized as a finding consistent with the fact that its color tone was reddish purple.
Furthermore, when Patent Document 1 was examined (Study Example 1 in the present specification), it was confirmed that the RSWP strain has a composition significantly different from that of the red sweet potato plant produced according to the present invention with respect to the composition relating to the acylation modification mode of the anthocyanin pigment. Was given. Specifically, it was recognized that the RSWP strain has a high content of hydroxybenzoic acid-modified anthocyanins as a main peak and a low content of hydroxycinnamic acid-modified anthocyanins that contributes to improved stability (Patent Document 1 Examples 1-3 and FIG. 1, etc.).

The present invention specifically relates to the invention described below.
[Item 1]
A sweet potato plant containing a pelargonidin-based anthocyanin compound as a pigment component having the characteristics described below:
(1) A sweet potato plant belonging to the crossing group of Kyushu 89360-8 (FERM BP-22365) and Kyushu 294 (FERM BP-22364), or a progeny group whose parent is a sweet potato plant belonging to the crossing group It is a sweet potato plant,
(2) As the composition of the anthocyanin-based pigment contained in the root tuber, the peak area ratio of the pelargonidin-based anthocyanin compound in HPLC analysis is 70% or more with respect to the peak area of all the anthocyanin compounds, and
(3) As a composition of the anthocyanin dye contained in the root tuber, the peak area ratio of the anthocyanin compounds having a hydroxycinnamic acid modifying group in HPLC analysis is 50% or more with respect to the peak areas of all the anthocyanin compounds.
[Item 2]
Item 2. The sweet potato plant according to Item 1, which has the characteristics described below:
As the composition of the anthocyanin dye contained in the root tuber, the peak area ratio of the anthocyanin compound having a caffeic acid modifying group as R ₂ in the structural formula (I) in HPLC analysis is 50% or more with respect to the peak area of all the anthocyanin compounds. Indicates:

(In the structural formula, R ₁ represents a hydrogen atom, a hydroxyl group, or a methoxy group, R ₂ represents a hydrogen atom or a caffeic acid modifying group, R ₃ represents a hydrogen atom, a p-hydroxybenzoic acid modifying group, A caffeic acid-modifying group or a ferulic acid-modifying group is shown.

[Item 3]
Item 2. The sweet potato plant according to Item 1 or 2, which has the characteristics described below:
Regarding an anthocyanin dye composition obtained by extracting tuberous root with 0.3% sulfuric acid water, when an aqueous solution containing the anthocyanin dye composition and having a pH of 3 is prepared with a citrate buffer solution, visible light It has a maximum absorption wavelength in the region from 505 to 520 nm, and
Regarding the anthocyanin dye composition obtained by extracting tuberous root with 0.3% sulfuric acid water, the anthocyanin dye composition was prepared so that the color value E ^10% _{1 cm} value was 0.3 to 0.7. A _{320 nm} / A _{530 nm} value is 3 or more when an aqueous solution having a pH of 3 is prepared with a citrate buffer solution.
[Item 4]
A method for producing a sweet potato plant containing a pelargonidin-based anthocyanin compound as a pigment component, which comprises the following steps:
(1) A step of crossing 9 lines 89360-8 (FERM BP-22365) and 9 lines 294 (FERM BP-22364) to obtain sweet potato plants belonging to the crossing group, or crossing the sweet potato plants belonging to the crossing group The process of obtaining sweet potato plants belonging to the progeny group,
(2) As a composition of the anthocyanin-based pigment contained in the root tuber, a step of selecting one having a pelargonidin-based anthocyanin compound peak area ratio in HPLC analysis of 70% or more with respect to the peak areas of all anthocyanin compounds, and
(3) As the composition of the anthocyanin-based pigment contained in the root tuber, select the one in which the peak area ratio of the anthocyanin compound having a hydroxycinnamic acid modifying group in HPLC analysis is 50% or more with respect to the peak area of all the anthocyanin compounds. Process.
[Item 5]
The plant body of the sweet potato plant according to any one of Items 1 to 3.
[Item 6]
Item 5. The processed plant product, squeezed product, extract, or further processed product thereof according to Item 5.
[Item 7]
Sweet potato-derived anthocyanin-based pigment composition having the following characteristics:
As the composition of the anthocyanin-based dye, the peak area ratio of the pelargonidin-based anthocyanin compound in the HPLC analysis shows 70% or more with respect to the peak area of the total anthocyanin compound, and
As for the composition of the anthocyanin-based dye, the peak area ratio of the anthocyanin compound having a hydroxycaterous acid modifying group in the HPLC analysis shows 50% or more with respect to the peak area of all the anthocyanin compounds.
[Item 8]
Item 2. The sweet potato-derived anthocyanin-based pigment composition according to Item 7, which has the characteristics described below:
As the composition of the anthocyanin dye, the peak area ratio of the anthocyanin compound having a caffeic acid modifying group as R ₂ in the structural formula (I) in HPLC analysis is 50% or more based on the peak area of all the anthocyanin compounds:

(In the structural formula, R ₁ represents a hydrogen atom, a hydroxyl group, or a methoxy group, R ₂ represents a hydrogen atom or a caffeic acid modifying group, R ₃ represents a hydrogen atom, a p-hydroxybenzoic acid modifying group, A caffeic acid-modifying group or a ferulic acid-modifying group is shown.

[Item 9]
Sweet potato-derived anthocyanin-based pigment composition having the following characteristics:
When an aqueous solution containing the anthocyanin-based dye composition and having a pH of 3 is prepared with a citrate buffer, it has a maximum absorption wavelength of 505 to 520 nm in the visible light region, and
When an aqueous solution containing the anthocyanin dye composition and having a pH of 3 with a citric acid buffer solution was prepared so that the color value E ^10% _{1 cm} value was 0.3 to 0.7, the A _{320 nm} / A _{530 nm} value was obtained. 3 or more.
[Item 10]
Item 2. The anthocyanin-based pigment composition according to any one of Items 7 to 9, which has the following characteristics relating to absorption into the body:
The amount is 1.5 times or more the cell permeation amount of the anthocyanin dye composition derived from Ayamurasaki.
[Item 11]
Item 5. A method for producing a sweet potato-derived anthocyanin-based pigment composition, which comprises the step of using the plant as a raw material according to Item 5.

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide a sweet potato-derived anthocyanin-based pigment composition having a color-developing property exhibiting a bright red color tone and excellent compound stability.

FIG. 5 is a result diagram of a chromatogram obtained by subjecting a mass root extract of Kyushu No. 187 to HPLC / MS analysis in Example 1. It is a photographic image of a sample bottle filled with each test solution in the color development evaluation by pH change according to Example 4. It is a photographic image diagram which showed the observation result of the beverage test solution filled in the PET bottle in the light resistance test which concerns on Example 6. It is a result figure which measured the absorbance of the solution on the basement membrane side of a cell sheet with time in the intestinal membrane permeability test which concerns on Example 9. FIG. It is a photograph image of the sweet potato juice squeezed liquid produced in Example 10. It is a photograph image of the sweet potato paste produced in Example 11. It is a photograph image of the sweet potato powder produced in Example 12. It is a schematic diagram which showed the genealogy relation of the original variety used in the production process which concerns on this Example, and the progeny line obtained by the crossing thereof.

Hereinafter, embodiments of the present invention will be described in detail, but the technical scope of the present invention is not limited to aspects including all of the following configurations. Further, the technical scope according to the present invention does not exclude aspects including other configurations other than the configurations described below, unless they substantially hinder the effects of the technical features according to the present invention. Absent.
This application is an application with priority claim based on Japanese Patent Application No. 2019-038106 filed in Japan by the applicant, the entire contents of which are incorporated into the present application by reference.

In the present specification, the “anthocyanin compound” is a natural pigment compound derived from a plant raw material that exhibits a color tone of red, blue, purple, etc., and a sugar molecule (for example, glucose, galactose, rhamnose, etc.) is present in the anglycone anthocyanidin. Refers to bound glycosides. In addition to the sugar molecule, an organic acid (for example, sinapinic acid, caffeic acid, succinic acid, malonic acid, etc.) may bind to form an acylated anthocyanin. As for the structure of anthocyanin compounds, there are many molecular species having different modification modes depending on the combination of the type of aglycone, the type and binding position of sugar molecules, the presence or absence and type of organic acid, and the binding position. The anthocyanin compound has various different color tones and various properties due to the difference in these structures.
In the present specification, “anthocyanidin” refers to an aglycone of an anthocyanin compound, and a compound that forms a skeleton structure of an anthocyanin compound by binding with a sugar molecule or the like. The general structural formula of anthocyanidins can be expressed as the following structural formula (II). R ₂₁ to R ₂₇ in the structural formula (II) each represent a hydrogen atom (—H), a hydroxyl group (—OH), or a methoxy group (—OCH ₃ ).

Anthocyanidin has three ring structures of A ring, B ring, and C ring, and has a hydroxyl group (—OH) or a methoxy group (—OCH ₃ ) added to the B ring (benzene ring on the right side of the structural formula (II)). It is mainly classified into 6 strains of pelargonidin, cyanidin, delphinidin, peonidin, petunidin, and malvidin depending on the number. Other examples include auranthinidine, luteolinidin, europeinidine, rosinidine, and the like. These have various color tones when they become the constituent aglycones of the anthocyanin compound.

In the present specification, "An." may be used as an abbreviation for anthocyanin, "Pg" for pelargonidin, "Cy" for cyanidin, and "Pn" for paeonidine.
In the present specification, the description of "peak area" in the HPLC analysis can be read as a value reflecting the "content" of the compound. The term "peak area ratio" can be read as "content ratio".
In the present specification, the term “red sweet potato pigment” is used as a term indicating an anthocyanin pigment derived from the sweet potato plant according to the present invention, unless otherwise specified.
In the present specification, the term “purple sweet potato pigment” is used as a term indicating an anthocyanin pigment derived from the sweet potato variety “Ayamurasaki” unless otherwise specified. The purple sweet potato dye used as the comparative sample in the present specification can be prepared by the method described in Example 2.

1. 1. New Sweet Potato Plant The sweet potato plant according to the present invention belongs to Ipomoea batatas and has genetic characteristics not found in ordinary sweet potato varieties with respect to the metabolic system of anthocyanin compounds.

[Production means]
The sweet potato plant according to the present invention can be obtained by utilizing the genetic background obtained at a constant frequency by crossing the parent lines of 9-line 89360-8 (FERM BP-22365) and 9-line 294 (FERM BP-22364). It is possible to create.
That is, the sweet potato plant according to the present invention is a sweet potato plant belonging to a crossing group produced by crossing the parent lines 9-89360-8 (FERM BP-22365) and 9-294 (FERM BP-22364), or It belongs to a progeny group having a sweet potato plant belonging to the above-mentioned crossing group as a crossing parent.

Original varieties In the present invention, by selecting from a mating group obtained by crossing two specific original varieties, a pelargonidin-based anthocyanin compound that is not contained in a plant of a normal sweet potato variety line is used as a main pigment component. A sweet potato plant containing can be produced. In the present specification, the term “original variety” is used as a broad term that includes parent strains that are not registered as a variety.
The sweet potato plant according to the present invention has a metabolic mechanism in which the production and accumulation of cyanidin and peonidin are suppressed and the production and accumulation of pelargonidin are possible. On the other hand, 9-type 89360-8 (FERM BP-22365) and 9-type 294 (FERM BP-22364), which are the original varieties of the sweet potato plants according to the present invention, are cyanidin-type anthocyanins and peonydin-types, like the usual sweet potato varieties. It is a strain whose main pigment is anthocyanin, and is not a variety strain containing pelargonidin-based anthocyanin.
However, in the process of producing a sweet potato plant having a bright red-colored mass root according to the present invention, the mechanism of action in which a trait containing a pelargonidin-based anthocyanin, which is not found in the original varieties, is acquired is unknown. In the mating population generated by, the genetic background expressing the trait containing the pelargonidin anthocyanin occurs at a constant frequency. The pelargonidin-type anthocyanin-containing trait according to the present invention is presumed to be a special trait that is expressed due to the genetic background characteristic of the cross population of the above-mentioned two breeds of original varieties.

Moreover, the sweet potato plant according to the present invention has a characteristic that the content of the anthocyanin compound in which the anthocyanin compound is acylated with a hydroxycinnamic acid is significantly high. Specifically, R _{2 in} the general structural formula (I) of the anthocyanin compound has a remarkably high content of the anthocyanin compound acylated with caffeic acid which is a hydroxycinnamic acid compound, and controls caffeic acid acylation of the site. It has a metabolic function suitable for enzyme activity.
This trait is presumed to be a characteristic trait related to the acylation modification property derived from the genetic background of one of the original cultivars, Kyushu 89360-8 (FERM BP-22365), and found in other cultivars. It has not been.
Here, the original varieties Kyushu 89360-8 (FERM BP-22365) and Kyushu 294 (FERM BP-22364) are breeding lines originally created by the present inventors, and are not known or publicly available techniques. is not.

As shown in the above points, in the present invention, the pelargonidin-based anthocyanin compound is contained in a high amount by performing selection using the anthocyanin composition as an index by focusing on the mating population or the progeny population by the combination of the above two original varieties. It becomes possible to produce sweet potato plants.
In addition, in the past enormous breeding studies of sweet potatoes, there has been no report of a variety line having a trait containing a high content of pelargonidin anthocyanin having a modifying group of hydroxycinnamon acids possessed by the sweet potato plant according to the present invention. In this respect, it is presumed that it is difficult to obtain the sweet potato plant according to the present invention from the viewpoint of its genetic background from the combination of sweet potato varieties other than the above two original varieties.

Original breeding population or progeny population The sweet potato plants according to the present invention belong to the original breeding population or progeny population .
Here, the “breeding group” of the original variety refers to a first-generation offspring group obtained by crossing in which two lines of the original variety are combined. The mating population is a population created by combining the genetic backgrounds possessed by the two original strains, and at least in some of the individuals, the genetic backgrounds of the traits that enable accumulation of pelargonidin anthocyanins Individuals newly acquired and included are included. In addition, in the mating population, at least a part or all of the individuals have a trait having a high content of anthocyanin having a hydroxycinnamic acid modifying group.
Further, the "progeny group" refers to a child group and its descendant group obtained by using the individuals to which the mating group belongs as one or both of the mating parents. More specifically, a selfed population of individuals belonging to the mating population, a mating population of individuals belonging to the mating population with other breed lines, these selfed populations or further breeding populations of these with other breed lines, Etc. can be mentioned. The progeny group also includes these further progeny groups.

As the sweet potato plant according to the present invention, it is possible to obtain a sweet potato plant having a desired excellent trait by repeating self-propagation, allogeneic breeding, mating, selection and the like. Here, the excellent traits are, for example, traits in which the content of pelargonidin-based anthocyanins is further increased, traits in which the content of anthocyanins having a hydroxycaterous acid modifying group is further increased, and traits in which the content of anthocyanin compounds is further increased (for example, (Characteristics with high raw material color value), traits advantageous for the quality of processed products and manufacturing processes described below, and the like can be mentioned.

Production Method The present invention includes the invention relating to the method for producing sweet potato plants according to the present invention.
More specifically, the present invention includes a method for producing sweet potato plants belonging to Ipomoea batatas containing a pelargonidin-based anthocyanin compound as a pigment component, which invention includes the following steps.
(1) A step of crossing 9 lines 89360-8 and 9 lines 294 to obtain sweet potato plants belonging to a cross population, or a step of obtaining sweet potato plants belonging to a progeny population having a sweet potato plant belonging to the cross population as a cross parent
(2) As a composition of the anthocyanin-based pigment contained in the root tuber, a step of selecting one having a pelargonidin-based anthocyanin compound peak area ratio in HPLC analysis of 70% or more with respect to the peak areas of all anthocyanin compounds, and
(3) As the composition of the anthocyanin-based pigment contained in the root tuber, select the one in which the peak area ratio of the anthocyanin compound having a hydroxycinnamic acid modifying group in HPLC analysis is 50% or more with respect to the peak area of all the anthocyanin compounds. Process.

As the production step in the method, a step of selecting from the mating population of the original varieties described in the above paragraph or the progeny population can be used. Here, as the mating group and progeny group, the contents described in the above paragraph can be referred to and used. In addition, selfing, inbred allogeneic, mating, and/or selection or the like is performed or repeated to obtain a sweet potato plant expressing the above-mentioned characteristics and traits, and can be produced as a desired sweet potato plant.
As for the description regarding the HPLC analysis and the like in the selection step, the details described in the following paragraphs can be referred to and used.
Here, in the production method according to the present invention, it is possible to carry out the above-mentioned step (1) and then the above-mentioned step (2) and the above-mentioned (3). Further, a mode in which the step (2) and/or the step (3) is performed in the middle of the step (1) is also possible. Further, the step (2) and the step (3) can be performed simultaneously or separately. Further, the step (1), the step (2), and the step (3) can be performed plural times.

Method for determining whether to belong to a mating group or progeny group As an example of a method for determining whether a sweet potato plant according to the present invention belongs to a mating group or a progeny group of the original variety, a variety lineage identification marker or the like is used. Can be done by A genotype such as SNP (single nucleotide polymorphism) or SSR (microsatellite polymorphism) specific to the sweet potato plant according to the present invention can be used as an index as the identification marker that can be used for the determination.
In addition, in the case of a progeny population by crossing with another variety line, such an identification marker may not be directly available, but in the case of this aspect, the genetic background of the crossing parent etc. is comprehensively considered and judged. It is desirable to do.

[Anthocyanin compounds in new sweet potato plants]
In the sweet potato plant and anthocyanin pigment composition according to the present invention, the “total anthocyanin compound” used for the peak area calculation in the HPLC analysis refers to all the glycoside compounds having anthocyanidin as an aglycone. Here, in the sweet potato plant or the produced pigment composition, there may be an intermediate product lacking a sugar chain, fragmentation ion or the like according to the following structural formula (I), which was produced by the present invention. In the preferred embodiment of sweet potato plants, the amount of accumulation of these low molecular weight compounds is usually extremely small, and therefore the peak area value can be calculated without including them in all anthocyanin compounds.
Incidentally, as the sweet potato plant and anthocyanin composition according to the present invention, a mode in which these low molecular weight compounds produced in the sweet potato in vivo or generated in the composition preparation step are mixed in a quantity exceeding a trace amount or in a large amount. Is also included. In the case of the said aspect, calculation shall be performed considering these low molecular weight compounds as all the anthocyanin compounds.
In addition, in the present invention, when a composition in which a sugar chain is absent from the structural formula (I) is reduced to have a low molecular weight by treatment or storage for the purpose of decomposition, treatment such as decomposition or storage is performed. Also for the composition after the above, the calculation is performed as the composition converted into the amount of the compound of the structural formula (I) before the decomposition.

The general structural formula (I) of the main anthocyanin compounds contained in the sweet potato plant and the anthocyanin pigment composition according to the present invention is shown below.

In the structural formula, R ₁ represents a hydrogen atom, a hydroxyl group, or a methoxy group. Specifically, when R ₁ is a hydrogen atom, the structural formula (I) is a pelargonidin-based anthocyanin. When R ₁ is a hydroxyl group, the structural formula (I) is a cyanidin-based anthocyanin. When R ₁ is a methoxy group, the structural formula (I) is a peonidin-based anthocyanin. Further, as R ₁ , a mode in which an anthocyanin compound having another functional group bonded thereto is also allowed as long as it does not substantially adversely affect the color development characteristics and stability.

R ₂ in the structural formula (I) represents a hydrogen atom, a hydroxyl group, or an organic acid modifying group. As the organic acid modifying group, a hydroxycinnamon acid modifying group is preferable.
Examples of the anthocyanin compound contained in the sweet potato plant according to the present invention include a modification mode anthocyanin compound in which R ₂ is a hydrogen atom or a caffeic acid modifying group, and particularly, a modification mode anthocyanin that is a caffeic acid modifying group. It is preferably a compound. Further, in the present invention, an embodiment in which R ₂ contains a hydroxyl group, another organic acid modifying group or the like is also acceptable.

R ₃ in the structural formula (I) represents a hydrogen atom, a hydroxyl group, or an organic acid modifying group. As the organic acid modifying group, a hydroxycinnamon acid modifying group is preferable.
Examples of the anthocyanin compound contained in the sweet potato plant according to the present invention include anthocyanin compounds having a modification mode in which R ₃ is a hydrogen atom, a p-hydroxybenzoic acid modifying group, a caffeic acid modifying group, or a ferulic acid modifying group. .. In the present invention, examples of R _3, embodiments containing a hydroxyl group or other organic acids modifying group and the like are also acceptable.

Examples of the anthocyanin compound contained in the sweet potato plant according to the present invention include a compound having a molecular structure composed of the structural formula (I), and the compound of the structural formula (I) is in an ionic state or a salt state. Those having a molecular structure that has become stable are also included in the compounds of structural formula (I).
Further, as long as the color development characteristics and stability are not substantially adversely affected, the embodiment of the anthocyanin compound having a structural change or functional group substitution with respect to the structural formula (I) is permitted. For example, the functionality of any of an anthocyanin compound having a skeletal structure substituted with another anthocyanidin molecule, an anthocyanin compound having a skeletal structure substituted with another sugar molecule, and an anthocyanin compound having a skeletal structure lacking one of the sugar molecules. Aspects such as anthocyanin compounds having a group-substituted skeletal structure are also acceptable. In these cases, it is possible to calculate each peak area value for the following compositional properties so that the anthocyanin compound contains these compounds.

[Compositional characteristics of anthocyanin dyes]
The sweet potato plant according to the present invention has vivid red color-developing properties and compositional properties of anthocyanin pigments that are involved in the structural stability of the pigment compound. As the compositional characteristic, the sweet potato plant according to the present invention has a compositional characteristic in which the pelargonidin-based anthocyanin compound is highly contained and the hydroxycinnamic acid modification rate of the anthocyanin compound is high.

Compositional characteristics related to color development characteristics (pelargonidin-based anthocyanin content, etc.)
The sweet potato plant according to the present invention has a compositional property containing a high amount of a pelargonidin-based anthocyanin compound with respect to the composition of the anthocyanin-based pigment. On the other hand, the sweet potato plant according to the present invention has a compositional characteristic that the content of cyanidin-based anthocyanins and peonidin-based anthocyanins, which are main pigments in ordinary sweet potato plants, is low.

The sweet potato plant according to the present invention is recognized as a sweet potato plant in which the production and accumulation of cyanidin and peonidin are suppressed and the metabolic system capable of producing and accumulating pelargonidin has been functionally acquired. In this respect, the sweet potato plant according to the present invention is recognized as a sweet potato plant having a genetic background capable of accumulating a pelargonidin anthocyanin compound excellent in bright red color as a main pigment.
Due to the pigment composition, the sweet potato plant according to the present invention exhibits a bright red color that cannot be expressed by a normal sweet potato plant. Especially in the lump root, the bright red tone tends to be exhibited. The extract of the sweet potato plant exhibits a color tone close to orange-red in the acidic region, and exhibits a color tone extremely close to that of the radish pigment.

Here, since a normal sweet potato plant has a metabolic system in which pelargonidin-based anthocyanins are not synthesized and / or accumulated in vivo, the anthocyanin composition thereof is cyanidin-based anthocyanins and peonidin-based anthocyanins in terms of the type of aglycone of anthocyanins. It has compositional characteristics mainly composed of. As a color development characteristic, it reflects purple to reddish purple in the weakly acidic range, reflecting the composition of the compound.
In addition, although there are reports of strains containing pelargonidin-based anthocyanins (Patent Document 1, Non-Patent Document 1), there are examples of producing strains containing a high amount of pelargonidin-based anthocyanins with a composition similar to that of the sweet potato plant according to the present invention. unknown.

As the sweet potato plant according to the present invention, it is preferable that the composition of the anthocyanin pigment has a composition characteristic that the content of the pelargonidin anthocyanin compound is high.
That is, the sweet potato plant according to the present invention has the following characteristics:
As the composition of the anthocyanin-based pigment contained in the root tuber, the peak area ratio of the pelargonidin-based anthocyanin compounds in the HPLC analysis is 70% or more with respect to the peak areas of all the anthocyanin compounds.
If the ratio is 70% or more, it is judged to be good from the viewpoint of color development characteristics. Particularly, in consideration of vivid red color development, sweet potato plants having the ratio of 75% or more, more preferably 80% or more, and further preferably 85% or more are preferable.

Further, as the sweet potato plant according to the present invention, it is preferable that the composition of the anthocyanin pigment has a composition characteristic that the content of the anthocyanin compound containing anthocyanidin other than pelargonidin in its skeletal structure is low.
That is, the sweet potato plant according to the present invention has the following characteristics:
As the composition of the anthocyanin-based pigment contained in the root tuber, the total peak area ratio of the cyanidin-based anthocyanin compound and the paeonidine-based anthocyanin compound in the HPLC analysis is less than 30% with respect to the peak areas of all the anthocyanin compounds.
If the ratio is less than 30%, the purple tone is reduced and the color development characteristic is determined to be good from the viewpoint of the color development characteristic. Particularly, in consideration of the vivid red color development, sweet potato plants exhibiting the ratio of less than 25%, more preferably less than 20%, still more preferably less than 15% are preferable.

The above-mentioned ratio regarding the anthocyanin-based pigment composition is a value indicated by a value obtained by HPLC analysis of an extract of sweet potato lump root, but when the value is used as an index, the anthocyanin of the sweet potato plant according to the present invention is used. It is possible to evaluate the color development characteristics of the system dye composition by a measurement operation using a lump root extract.
Here, as the solvent for obtaining the extract, a dilute acidic aqueous solution (in one embodiment, 0.3 to 0.5% sulfuric acid water) can be mentioned.
Further, the peak area used in the above specification can be measured by HPLC analysis using a column (ODS column) packed with silica gel surface-modified with an octadecylsilyl group as a stationary phase, for example. is there. Further, as the analysis condition, a gradient condition using 0.1% formic acid water/0.1% formic acid-containing acetonitrile as a mobile phase, and elution conditions of a flow rate of 0.2 mL/min and a temperature of 40° C. may be adopted. It is possible. 530 nm can be adopted as the detection wavelength.
As a specific method and various conditions for the HPLC analysis, it is possible to adopt a mode capable of detecting and separating each peak indicating an anthocyanin compound, but as one mode, the method described in Example 1 is used. And conditions can be adopted. It is also possible to adopt a method and conditions according to this.

The anthocyanin pigment composition of the sweet potato plant according to the present invention includes a composition containing an anthocyanin compound having an anthocyanidin as a skeleton structure other than the above three types, as long as the color development characteristics and stability are not substantially adversely affected. included.

Compositional properties related to compound stability (acyllation modification rate)
The sweet potato plant according to the present invention has a compositional property that the anthocyanin pigment is highly contained in the composition of the anthocyanin pigment, the anthocyanin compound being acylated with a phenolic acid.

Here, examples of the phenolic acids that acylate and modify the anthocyanin compound include hydroxy katsura acids and hydroxybenzoic acids, and examples of the phenolic acids that contribute to improving the stability of the dye compound include hydroxy, which has high antioxidant activity. The cinnamic acids are preferred.
The anthocyanin compound of the sweet potato plant according to the present invention has a very high acylation modification rate with hydroxycinnamon acids, while a low acylation modification rate with hydroxybenzoic acids.
That is, the sweet potato plant according to the present invention is characterized by having a high content of an anthocyanin compound acylated with hydroxycinnamon acids. In addition, the sweet potato plant according to the present invention is characterized by having a low content of anthocyanin compounds acylated with hydroxybenzoic acids.
Regarding the metabolic pathway of the anthocyanin compound, the sweet potato plant according to the present invention is recognized as a sweet potato plant having a strong metabolic activity controlling acylation modification with hydroxy cascading acids, and a weak metabolic activity controlling acylation modification with hydroxybenzoic acid. To be In this respect, the sweet potato plant according to the present invention is recognized as a sweet potato plant having a genetic background capable of accumulating an anthocyanin compound acylated with hydroxycinnamon acids having high antioxidant activity as a main pigment. Due to the compositional characteristics, the anthocyanin-based pigment of the sweet potato plant according to the present invention exhibits a function excellent in the stability of its color tone.

Here, the RSWP strain, which is a pelargonidin-based anthocyanin strain described in the prior art, shows a modification mode opposite to that of the sweet potato plant according to the present invention with respect to the acylation modification mode of phenolic acids, and is a hydroxy cinnamic acid having high antioxidant activity. It has a compositional feature of low acylation modification rate and high acylation modification rate of hydroxybenzoic acids (Patent Document 1). In this respect, the RSWP-based anthocyanin-based dyes are not recognized as having high compound stability in terms of compositional characteristics.

As the sweet potato plant according to the present invention, it is preferable to use, as a composition of the anthocyanin pigment, a plant having compositional characteristics with a high modification rate of hydroxycinnamic acids.
That is, the sweet potato plant according to the present invention has the following characteristics:
As the composition of the anthocyanin-based pigment contained in the root tuber, the peak area ratio of the anthocyanin compound having a hydroxycinnamic acid modifying group in HPLC analysis is 50% or more with respect to the peak area of all the anthocyanin compounds.
As for the ratio, if it is 50% or more, the antioxidant activity is strong and it is judged to be good from the viewpoint of compound stability. In particular, the ratio is 55% or more, more preferably 60% or more, still more preferably 65% or more, even more preferably 70% or more, particularly preferably 75% or more, even more preferably 80% or more, still more particularly preferably. Is preferably a sweet potato plant showing 85% or more.

Here, in the present invention, the hydroxycinnamonic acid modifying group serving as the acylation modifying group may be a modifying group represented by the following structural formula (III). Here, the structural formula (III) shows the structure of the functional group portion of the structure in which the carbon atom of the carboxyl residue on the left side of the structural formula is bonded to the sugar molecule in the anthocyanin compound. Specifically, structural formula (III) refers to a functional group corresponding to R ₂ or R ₃ in structural formula (I).
The hydroxycinnamic acid-modifying group according to the present invention has, for example, a hydroxyl group in any of R ₃₁ to R _{33 in} the structural formula and a hydrogen atom, a hydroxyl group, a methoxy group, or a prenyl group in the remaining side chain. And the like.

The anthocyanin compound contained in the sweet potato plant according to the present invention is preferably an anthocyanin compound modified with caffeic acid or ferulic acid as hydroxycinnamic acids serving as an acylating modification group. That is, as the hydroxycinnamic acid-modifying group according to the present invention, a mode that is a caffeic acid-modifying group or a ferulic acid-modifying group can be preferably cited.
Here, as the caffeic acid (3,4-dihydroxycinnamic acid) modifying group, in the structure of the functional group represented by the structural formula (III), R ₃₁ is a hydroxyl group, R ₃₂ is a hydroxyl group, and R ₃₃ is a hydrogen atom. It can be shown as a functional group. The ferulic acid-modifying group can be shown as a functional group in which R ₃₁ is a methoxy group, R ₃₂ is a hydroxyl group, and R ₃₃ is a hydrogen atom in the structure of the functional group represented by the structural formula (III).
Moreover, as the hydroxycinnamic acids other than the two types of caffeic acid and ferulic acid, which are the acylating modification groups, an embodiment in which hydroxycinnamic acids having a strong antioxidant activity is used as the modification group is also possible. For example, as the hydroxycinnamic acid-modifying group according to the present invention, various caffeic acid isomers such as dihydroxycinnamic acid, various ferulic acid isomers such as isoferric acid, p-coumaric acid, sinapinic acid, artepirin C, rosmarinic acid, etc. Modification groups of, but are not particularly limited to these.

Further, as the sweet potato plant according to the present invention, a plant having a composition property with a low hydroxybenzoic acid modification rate is preferable as the composition of the anthocyanin pigment.
That is, the sweet potato plant according to the present invention has the following characteristics:
As the composition of the anthocyanin-based dye contained in the clump root, the peak area ratio of the anthocyanin compound having a hydroxybenzoic acid modifying group in the HPLC analysis shows less than 50% with respect to the peak area of the total anthocyanin compound.
If the ratio is less than 50%, it is judged to be good from the viewpoint of compound stability. In particular, the ratio is less than 45%, more preferably less than 40%, even more preferably less than 35%, even more preferably less than 30%, particularly preferably less than 25%, even more preferably less than 20%, even more particularly preferably. Sweet potato plants showing less than 15% are suitable.

Here, in the present invention, the hydroxybenzoic acid modifying group serving as the acylation modifying group may be a modifying group represented by the following structural formula (IV). Here, the structural formula (IV) shows the structure of the functional group portion of the structure in which the carbon atom of the carboxyl residue on the left side of the structural formula is bound to the sugar molecule side in the anthocyanin compound. Specifically, the structural formula (IV) refers to a functional group corresponding to R ₂ or R ₃ in the structural formula (I).
Examples of the hydroxybenzoic acid-modifying group according to the present invention include a functional group having a hydroxyl group in any of R ₄₁ to R _{43 in} the structural formula and a hydrogen atom or a hydroxyl group in the remaining side chain. be able to.

Examples of the anthocyanin compound contained in the sweet potato plant according to the present invention include those modified with p-hydroxybenzoic acid as hydroxybenzoic acids serving as acylation modifying groups. That is, as the hydroxybenzoic acid-modifying group according to the present invention, a preferred embodiment is a p-hydroxybenzoic acid-modifying group. Here, as the p-hydroxybenzoic acid (4-hydroxybenzoic acid) modifying group, in the structure of the functional group represented by the structural formula (IV), R ₄₁ is a hydrogen atom, R ₄₂ is a hydroxyl group, and R ₄₃ is a hydrogen atom. Can be shown as a functional group.
In addition, a mode in which a hydroxybenzoic acid other than the compound is used as the modifying group is also possible as long as there is substantially no adverse effect on the color development characteristics, stability, etc. For example, examples of the hydroxybenzoic acid modifying group according to the present invention include, but are not limited to, various modifying groups such as various hydroxybenzoic acids, which are p-hydroxybenzoic acid isomers, gallic acid, and protocatechuic acid. ..

The anthocyanin compound contained in the sweet potato plant according to the present invention also includes an anthocyanin compound acylated and modified with an organic acid other than phenolic acids, as long as there is no substantial adverse effect on color development characteristics, stability and the like. Examples of the organic acid serving as an acylation modifying group include, but are not limited to, malonic acid, succinic acid and the like.

In the anthocyanin pigment compound of the sweet potato plant according to the present invention, it is preferable that R ₂ and/or R ₃ in the structural formula (I) is an acylation modification site as the acylation modification site. That is, an anthocyanin compound in which R ₂ and / or R ₃ in the structural formula (I) is an acylation modifying group is suitable. In addition, a composition containing an anthocyanin compound in which modification sites other than R ₂ and R ₃ in the structural formula (I) are acylated and modified is also acceptable, as long as the coloring property and stability are not substantially adversely affected. ..

As the anthocyanin-based pigment composition of the sweet potato plant according to the present invention, it is preferable that the compositional characteristics related to the acylation modification rate show the above ratio as the ratio to the total anthocyanin compounds. In particular, the modification ratio of hydroxycinnamic acids having the above ratio is suitable. Further, as the anthocyanin-based pigment composition of the sweet potato plant according to the present invention, one having the same tendency as the above-mentioned compositional properties regarding the acylation modification rate is preferable regardless of the type of aglycone.

The above-mentioned ratio relating to the anthocyanin pigment composition is a value indicated by a value obtained by HPLC analysis of an extract of tuberous root of sweet potato, when the value is used as an index, the anthocyanins of the sweet potato plant according to the present invention. It is possible to evaluate the compound stability of the system dye composition by a measurement operation using a lump root extract.
Here, the method and various conditions for performing the HPLC analysis can be performed by a conventional method, but as an example of the embodiment, the method and conditions for the HPLC analysis described in the above paragraph, and the description of Example 1 are given. It can be done in the same way as.

Compositional properties related to compound stability (caffeic acid modification rate at specific modification sites)
Regarding the composition of the anthocyanin pigment, the sweet potato plant according to the present invention has a composition characteristic that the anthocyanin compound in which R ₂ in the general structural formula (I) is a caffeic acid modifying group is highly contained. Here, caffeic acid is a compound having high antioxidant activity among hydroxycinnamic acids. Therefore, the caffeic acid-modifying group has a high potency for improving stability and is a suitable acylating modification group.

The sweet potato plant according to the present invention has a strong metabolic activity that controls acylation modification with hydroxycinnamic acids with respect to the metabolic pathway of anthocyanin compounds, and in particular, R ₂ in general structural formula (I) is modified with caffeic acid. It is recognized as a sweet potato plant with strong metabolic activity. In this respect, the sweet potato plant according to the present invention is recognized as a sweet potato plant having a genetic background in which anthocyanin compounds acylated with caffeic acid having a high antioxidative activity can be accumulated as a main pigment. This characteristic is advantageous for exhibiting dye stability in the anthocyanin production pathway.
Due to the compositional characteristics, the anthocyanin pigment of the sweet potato plant according to the present invention exerts a function excellent in stability of the color tone.

As the sweet potato plant according to the present invention, a plant having a high caffeic acid modification rate at a specific modification site is preferable as the composition of the anthocyanin pigment.
That is, the composition of anthocyanin dye contained in tuberous roots of sweet potato plant according to the present invention, the peak area ratio of the anthocyanin compound having caffeic acid modifying group as R ₂ in the structural formula (I) in the HPLC analysis, the total anthocyanin compound It shows 50% or more with respect to the peak area of.
If the ratio is 50% or more, it is judged to be good from the viewpoint of compound stability. In particular, sweet potato plants having a ratio of 55% or more, more preferably 60% or more, still more preferably 65% or more, still more preferably 70% or more, and particularly preferably 75% or more are preferable.

Anthocyanin dye composition contained in the sweet potato plant according to the present invention, where the composition comprising the anthocyanin compound to acylation modification sites to R ₂ of formula (I) are preferred, substantially in the color characteristics and stability As long as there is no adverse effect on the above, an embodiment containing an anthocyanin compound in which the site corresponding to R _{2 of the} anthocyanin compound having a structural change or functional group substitution with respect to the structural formula (I) is an acylation modification site is permitted. .. For example, the functionality of any of an anthocyanin compound having a skeletal structure substituted with another anthocyanidin molecule, an anthocyanin compound having a skeletal structure substituted with another sugar molecule, and an anthocyanin compound having a skeletal structure lacking one of the sugar molecules. Compositions containing anthocyanin compounds having a skeletal structure with substituted groups, etc. are acceptable.
In these cases, it is possible to calculate each peak area value relating to the above-mentioned compositional characteristics by including these compounds in the anthocyanin compound.

In the anthocyanin-based pigment composition contained in the sweet potato plant according to the present invention, it is preferable that the compositional characteristics regarding the caffeic acid modification rate of R ₂ show the same tendency as described above regardless of the type of aglycone.

The above-mentioned ratio relating to the anthocyanin pigment composition is a value indicated by a value obtained by HPLC analysis of an extract of tuberous root of sweet potato, when the value is used as an index, the anthocyanins of the sweet potato plant according to the present invention. It is possible to evaluate the compound stability of the system dye composition by a measurement operation using a lump root extract.
Here, the method and various conditions for performing the HPLC analysis can be performed by a conventional method, but as an example of the embodiment, the method and conditions for the HPLC analysis described in the above paragraph, and the description of Example 1 are given. It can be done in the same way as.

[Characteristics related to absorbance]
In the sweet potato plant according to the present invention, it is possible to use as an index the value regarding the absorbance reflecting the compositional characteristics of the bright red color-developing characteristics of the anthocyanin-based pigment composition and the structural stability of the pigment compound.

Absorbance characteristics related to color development characteristics (maximum absorption wavelength)
The sweet potato plant according to the present invention can use a value related to absorbance reflecting the compositional characteristics as an index for the bright red color-developing characteristics of the anthocyanin pigment composition.
That is, the sweet potato plant according to the present invention has the following characteristics:
With respect to the anthocyanin-based pigment composition obtained by extracting the lump root with 0.3% sulfuric acid water as a raw material, when an aqueous solution of pH 3 was prepared with a citrate buffer containing the anthocyanin-based pigment composition, visible light. It has a maximum absorption wavelength in the region of 505 to 520 nm.
Here, the value of the maximum absorption wavelength is a value indicating the color tone of the dye composition, and the closer the value is to the maximum absorption wavelength of the red radish dye, the more preferable a value is a bright red color tone. Specifically, it is judged to be good if it is 505 to 520 nm which is the maximum absorption wavelength range of the red radish dye at pH 3 (4th edition voluntary standard for existing additives). Particularly, sweet potato plants having the value at pH 3 of 505 to 518 nm, preferably 508 to 518 nm, and more preferably 510 to 514 nm are suitable.

The value of the maximum absorption wavelength of the sweet potato plant according to the present invention is a value reflecting the compositional characteristics of high content of the pelargonidin-based anthocyanin compound, and the extract of the sweet potato plant exhibits a color tone close to orange-red in the acidic region. , Has a color tone very close to that of the red radish dye.
When a large amount of cyanidin-based anthocyanin or the like is contained like ordinary purple sweet potato, the maximum absorption wavelength at pH 3 is around 520 to 540 nm, and the color tone is close to purple in the acidic region.
When the value is used as an index, it becomes possible to evaluate the color development characteristics of the sweet potato plant according to the present invention by a simple absorbance measurement operation using an extract of tuberous root.

Absorbance characteristics related to compound stability (A _{320 nm} / A _{530 nm} value)
The sweet potato plant according to the present invention can use, as an index, the compound stability of the anthocyanin pigment composition, and the value relating to the absorbance reflecting the compositional characteristics thereof.
That is, the sweet potato plant according to the present invention has the following characteristics:
Regarding the anthocyanin dye composition obtained by extracting tuberous root with 0.3% sulfuric acid water, the anthocyanin dye composition was prepared so that the color value E ^10% _{1 cm} value was 0.3 to 0.7. A _{320 nm} / A _{530 nm} value is 3 or more when an aqueous solution having a pH of 3 is prepared with a citrate buffer solution.
_{Here, A 320nm /} A _530nm value _is a value indicating the ratio of _{A 320 nm} (absorbance at the maximum absorption wavelength in the vicinity of a wavelength of 320 nm) and _{A 530 nm} (absorbance at the maximum absorption wavelength in the vicinity of a wavelength of 530 nm). A _{320 nm} indicates the absorption wavelength of the structure related to hydroxycinnamic acids, and A ₅₃₀ nm indicates the absorption wavelength of the anthocyanin compound. _Therefore , it can be determined that the higher the value, the higher the content of the structure related to hydroxycinnamic acids. That is, the higher the value, the more suitable anthocyanin-based dye composition in terms of compound stability.
Specifically, if the A _{320 nm} / A _{530 nm} value is 3 or more, it is judged to be good. Particularly, a sweet potato plant having a value of 3.1 or more, preferably 3.2 or more, more preferably 3.3 or more, still more preferably 3.4 or more at pH 3 is suitable.

Since the value of the absorbance of the sweet potato plant according to the present invention reflects the compositional characteristics of containing a high amount of the anthocyanin compound having a hydroxycinnonic acid modifying group, the anthocyanin-based pigment composition of the sweet potato plant is compound stable. Demonstrates high characteristics.
When the value is used as an index, the stability of the anthocyanin pigment composition of the sweet potato plant according to the present invention can be evaluated by a simple absorbance measurement operation using a tuber root extract.

[Characteristics related to anthocyanin content]
The sweet potato plant according to the present invention has the property of accumulating the anthocyanin pigment having the above-mentioned compositional characteristics in the plant body. Particularly, those having a property of containing anthocyanin dye at a high concentration in the root tuber (a property of high raw material color value) are preferable.
That is, the sweet potato plant according to the present invention has the following characteristics:
When an extract extracted from a mass root as a raw material with 0.3 to 0.5% sulfuric acid water is obtained, the color value E ^10% _{1 cm} value of the extract in terms of 1 g of the raw material is 0.5 or more.
Here, the measured value of the color value E ^10% _{1 cm} value indicates that the higher the value, the higher the accumulated content of the anthocyanin compound in the plant, and therefore it is suitable for use as a raw material for various products. Can be judged. Particularly, if the color value E ^10% _{1 cm} value is 0.5 or more, it is judged to be good. Particularly, sweet potato plants having the value of 1 or more, preferably 2 or more, and more preferably 3 or more are suitable.

Using the color value E ^10% _{1 cm} value as an index, it is possible to evaluate the anthocyanin compound content in the mass root of sweet potato, which is a storage organ. It is also possible to use the value as an index to estimate the content of anthocyanin compounds in other organs / tissues of the plant or the whole plant.
In the present invention, it is preferable to use a plant body (particularly a lump root) of a sweet potato plant having a high color value E ^10% _{1 cm} value as a raw material for various products. In addition, even in sweet potato plants having a low color value, if the compositional characteristics of the anthocyanin pigment satisfy the above-mentioned characteristics, it can be effectively used as a parent for transduction such as intermediate mothers. Is.

[Other characteristics]
The sweet potato plant according to the present invention is preferably a plant having, in addition to the above-mentioned characteristics, suitable traits that are advantageous for the quality of the processed product of the plant including the pigment preparation and the manufacturing process. ..
Here, examples of suitable traits include arbitrary traits that are advantageous for cultivation characteristics, quality of harvested products, processed sweet potato products, and the like. For example, traits that improve the quality of lump roots (eg, lump root size, lump root shape, lump root soft tissue density, etc.), traits related to lump root flavor and nutrition (composition related to flavor substances, sugar composition and content, vitamin content, etc.) Etc.), environmental resistance traits (eg, cold resistance, heat resistance, etc.), disease resistance traits, growth traits (eg, early-late cultivation period, plant hormone synthesis system, etc.), reproduction-related traits (eg, flowering) Control, self-incompatibility, cytoplasmic male sterility, etc.), etc.), but are not particularly limited to these.
As an example, from the viewpoint of the yield yield of the pigment composition, it is preferable that the clump root has an enlarged shape, the clump root tissue is clogged, or the like. From the viewpoint of the quality of food raw materials, traits related to the flavor of lump roots, traits related to nutritional components of lump roots, and the like are considered to be suitable. In addition, from the viewpoint of reducing labor for supplying raw materials, if the traits have excellent growth characteristics, environmental resistance, disease resistance, etc., the raw materials can be easily produced as agricultural products. Recognized as suitable.

[New sweet potato variety line]
In the present invention, it is possible to obtain a population of sweet potato plants having the above-mentioned characteristics and produce a sweet potato line. Further, in the present invention, it is also possible to produce sweet potato varieties from the strain.
That is, the present invention includes inventions relating to sweet potato varieties or lines, which contain the above-mentioned sweet potato plants as a group constituent individual or become a group constituent individual.

The present invention includes an invention relating to a method for producing a variety or line having the above-mentioned sweet potato plant as a constituent individual of a population. As a production step in the method, a population of sweet potato plants expressing the above-mentioned characteristics and traits is obtained by performing or repeating self-fertilization, allogeneic cross-breeding, mating, and / or selection using the sweet potato plants described in the above paragraph. Then, it becomes possible to produce a desired sweet potato variety or line. These specific methods can be used with reference to the contents described in the above paragraphs and examples.

Examples of the red sweet potato strain produced from the crossed population of the above two original varieties include nine series 06353-19, which has a particularly high content of pelargonidin anthocyanin and excellent redness. In addition, Kyushu No. 187, 9 series 14258-2, 9 series 14259-13, etc., which are progeny lines and have excellent mass root hypertrophy traits, can be mentioned, but are not particularly limited thereto.

[Plants and their use]
The present invention includes an invention relating to a plant body of a sweet potato plant having the above-mentioned characteristics or a processed product thereof.

Plant Body The plant body of the sweet potato plant according to the present invention can be effectively used in various fields, and can be particularly preferably used as a raw material of an anthocyanin pigment composition. That is, the present invention includes the above-mentioned invention relating to the plant body of the sweet potato plant.
The plant according to the present invention includes all the tissues and organs of the plant constituting the above-mentioned sweet potato plant. In addition, any part of the whole or a part of the plant body can be used. In addition, tissues and organs constituting the plant body can be used. In addition, plants at various developmental stages can be used.
For example, roots, roots, leaves, petioles, vines, stems, buds, flowers, fruits, seeds, seedlings and the like can be mentioned. Moreover, the whole or a part of the plant body including these can be mentioned.

As the plant body of the sweet potato plant according to the present invention, tuberous roots (particularly, enlarged roots and potatoes as storage organs) can be preferably used in consideration of use in agricultural crops or processed products. In particular, mature and enlarged mass roots are suitable. In the root tuber, since the anthocyanin pigment compound is accumulated at a high content, it can be suitably used as a raw material for an anthocyanin pigment composition and various processed products.
That is, as the plant body according to the present invention, a part or all of the tuber root, or a portion in which the plant body contains the tuber root can be preferably mentioned. In particular, as the plant body according to the present invention, a part or all of the mass roots can be preferably mentioned.

The present invention includes a method for cultivating a plant body of a sweet potato plant according to the present invention. That is, in the present invention, there is an invention relating to a method for cultivating a sweet potato plant, which comprises using the seeds or plants of the sweet potato plant described above or the sweet potato plant obtained by the production method described above. included.
In addition, the present invention includes a method for producing a plant body of a sweet potato plant according to the present invention. That is, the present invention includes an invention relating to a method for producing a sweet potato plant, which comprises using the sweet potato plant described above or a sweet potato plant obtained by the production method described above.
As the cultivation method and the production method of the plant body, it is possible to adopt a conventional method except that the plant body tissues such as seeds (seed, seedlings, etc.) and roots of the sweet potato plant according to the present invention are used. ..

Processed product The processed product of the sweet potato plant according to the present invention can be effectively used in various fields, and can be particularly preferably used as an anthocyanin-based pigment composition or a product using the same. That is, the present invention includes an invention relating to a processed product of the plant body of the above-mentioned sweet potato plant.
Specifically, the present invention includes inventions relating to the processed products, juices, extracts, or further processed products of the sweet potato plants described above.

The processed products according to the present invention include all processed products obtained by subjecting the plant body as a raw material to some processing. The processed products include those of various processing and processing stages, and as an example, squeezed products of plant bodies (including squeezed juice, semi-solids, solid products, etc.), plant extracts ( Extracts, semi-solids, solids, etc.), processed products of plants themselves (cut products of plants, crushed products, paste products, puree products, dried products, powder products, heat-treated products, frozen treatments) Products, various physical treatments, various chemical treatments, various enzyme treatments, etc.), products obtained by further processing the various processed products (secondary processed products or subsequent processed products), and the like.
Further, as specific examples of these processed products, sweet potato processed foods using tuber harvest, juice, beverages such as juice using the juice, pastes of plants, puree of plants Examples thereof include products, dried plants, extracts of plants, pigment compositions, pigment preparations, dyes, and colored processed products (for example, colored foods and drinks) using the various products.
In addition, other than the above, if it is recognized as equivalent to the processed product using the plant body of the sweet potato plant according to the present invention, it is included in the processed product according to the present invention.

The processed product according to the present invention includes not only a lump root but also a processed product made from either the whole plant or a part (for example, foliage, etc.), but it is particularly preferable that the raw material is a lump root. To be done.
Further, the present invention includes inventions relating to the above-described method for manufacturing various processed products. That is, the present invention is characterized in that the above-mentioned sweet potato plant or the plant of the sweet potato plant obtained by the above-mentioned production method is used as a raw material, which is a processed product, juice, or extraction of the sweet potato plant. And inventions relating to methods for manufacturing these or further processed products.
As a method for producing the processed product, a conventional method can be used except for using the plant body of the sweet potato plant according to the present invention.

2. In the anthocyanin dye composition of the present invention, by using the sweet potato plant according to the present invention as a raw material, it has a coloring property of exhibiting a bright red color tone in an acidic region, and the compound sweetness is excellent. It is possible to produce a derived anthocyanin dye composition.

The pigment composition according to the present invention refers to a pigment composition containing the anthocyanin compound of the sweet potato plant according to the present invention described above. For example, it refers to an anthocyanin-based pigment composition in which a part of the raw material contains the plant body of the sweet potato plant according to the present invention described above, or the entire raw material is the plant body of the sweet potato plant according to the present invention described above.
Here, in the present specification, the "anthocyanin-based pigment composition" refers to a composition containing an anthocyanin compound as a pigment component thereof, and also includes a composition containing a dye other than the anthocyanin compound and various compounds. Be done.
The anthocyanin-based pigment composition according to the present invention includes various processed products of sweet potato plants described in the above paragraph.

The anthocyanin-based dye composition according to the present invention does not exclude features other than the following features unless it substantially interferes with the effects of the technical features according to the present invention. .. Further, the technical scope according to the present invention is not limited to the embodiment including all of the following features except for the essential technical features.
Further, the measurement wavelength, method, conditions and the like for performing the following HPLC analysis on the anthocyanin-based pigment composition can be carried out in the same manner as described in the above paragraph on sweet potato plants.

[Compositional characteristics related to color development characteristics]
The anthocyanin-based dye composition according to the present invention exhibits coloring properties derived from the anthocyanin-based dye composition of the sweet potato plant as a raw material. As the compositional properties, the sweet potato-derived anthocyanin-based pigment composition according to the present invention has a high content of pelargonidin-based anthocyanin compound and a high compositional property of the anthocyanin compound with a high hydroxycinnamon acid modification rate.
In addition, about the suitable aspect and/or technical feature in embodiment shown below, it can be used with reference to the description of the above-mentioned paragraph regarding a sweet potato plant.

Compositional characteristics related to color development characteristics (pelargonidin-based anthocyanin content, etc.)
The anthocyanin-based pigment composition according to the present invention has a compositional feature of having a high content of a pelargonidin-based anthocyanin compound and a low content of a cyanidin-based anthocyanin or a peonidin-based anthocyanin.
Due to the dye composition, the anthocyanin dye composition according to the present invention exhibits a vivid red color development that cannot be expressed by a normal sweet potato dye. Especially in the acidic region, it exhibits a color tone close to orange-red and is extremely close to a radish pigment.

As the anthocyanin-based pigment composition according to the present invention, one having a compositional characteristic having a high content of a pelargonidin-based anthocyanin compound is preferable.
That is, the sweet potato-derived anthocyanin-based pigment composition according to the present invention has the following characteristics:
As the composition of the anthocyanin-based dye, the peak area ratio of the pelargonidin-based anthocyanin compound in the HPLC analysis shows 70% or more with respect to the peak area of the total anthocyanin compound.
If the ratio is 70% or more, it is judged to be good from the viewpoint of color development characteristics. In particular, in consideration of the point of vivid red color development, those having a ratio of 75% or more, more preferably 80% or more, still more preferably 85% or more are preferable.

Further, as the anthocyanin-based dye composition according to the present invention, one having a composition characteristic that the content of anthocyanin compounds other than pelargonidin in the skeletal structure thereof is low is suitable.
That is, the sweet potato-derived anthocyanin-based pigment composition according to the present invention has the following characteristics:
As the composition of the anthocyanin dye, the total peak area ratio of the cyanidin anthocyanin compound and the paeonidine anthocyanin compound in the HPLC analysis is less than 30% with respect to the peak areas of all the anthocyanin compounds.
If the ratio is less than 30%, the purple tone is reduced and the color development characteristic is determined to be good from the viewpoint of the color development characteristic. Particularly, in consideration of vivid red color development, it is preferable that the ratio is less than 25%, more preferably less than 20%, and more preferably less than 15%.

Absorbance characteristics related to color development characteristics (maximum absorption wavelength)
The anthocyanin-based dye composition according to the present invention is a composition showing a value regarding absorbance reflecting the above-mentioned compositional characteristics with respect to its bright red color-developing characteristics.
That is, the sweet potato-derived anthocyanin-based pigment composition according to the present invention has the following characteristics:
When an aqueous solution having a pH of 3 is prepared with a citrate buffer solution containing the anthocyanin-based dye composition, it has a maximum absorption wavelength of 505 to 520 nm in the visible light region.
Here, the value of the maximum absorption wavelength is a value indicating the color tone of the dye composition, and the closer the value is to the maximum absorption wavelength of the red radish dye, the more preferable a value is a bright red color tone. Specifically, it is judged to be good if it is 505 to 520 nm which is the maximum absorption wavelength range of the red radish dye at pH 3 (4th edition voluntary standard for existing additives). In particular, those having a pH of 505 to 518 nm, preferably 508 to 518 nm, and more preferably 510 to 514 nm are preferable.

The value of the maximum absorption wavelength of the anthocyanin-based dye composition according to the present invention is a value that reflects the compositional characteristics that the pelargonidin-based anthocyanin compound is highly contained.
When the value is used as an index, it becomes possible to evaluate the color forming characteristics of the anthocyanin dye composition according to the present invention by a simple absorbance measurement operation.

[Color development characteristics and overall color tone]
The anthocyanin-based pigment composition according to the present invention exhibits coloring characteristics exhibiting a bright red color tone due to the compositional characteristics of the anthocyanin compound of the sweet potato plant as a raw material.
As the color tone of the anthocyanin-based pigment composition according to the present invention, it exhibits a reddish purple to yellowish red color tone at pH 3.
Further, when expressed by the HUE value (or JIS color name) in the Munsell color system, it exhibits a hue of 10P (reddish purple) to 5RP (reddish purple) to 10RP (purple red) to 5R (red). It is a dye composition. A dye composition preferably exhibits a hue of 5RP (magenta) to 10RP (purple red) to 5R (red) when expressed by the HUE value (or JIS color name) in the Munsell color system.
When the dye composition according to the present invention is prepared in powder form, it tends to have a slightly blackish color tone. Further, in a solution in the neutral to alkaline range, the color tone to be exhibited tends to change to a bluish / greenish color tone.

In the anthocyanin-based pigment composition according to the present invention, as each value of the Hunter Lab color system, the "a value" indicating the red hue and the "b value" indicating the yellow hue are values close to the values indicated by the red dicon dye. It is preferable to have. Preferably, in addition to these, the "L value" indicating the lightness and the "CHROMA value" indicating the saturation are also preferably close to the values indicated by the red radish dye.

As the anthocyanin-based dye composition according to the present invention, one having a color-developing characteristic close to that of a red radish dye having a bright red color tone in an acidic region is preferable.
That is, the sweet potato-derived anthocyanin-based pigment composition according to the present invention preferably has the characteristics described below:
When an aqueous solution containing the anthocyanin dye composition is prepared using 0.3% citric acid water so that the color value E is ^{10% and the} _{1 cm} value is 80%, the anthocyanin dye composition is prepared. The color difference ΔE value from the aqueous solution prepared in the same manner as described above is 10 or less except that the red dicon dye is blended instead of.
Here, the red radish dye is an anthocyanin-based dye composition derived from red radish having a maximum absorption wavelength at 513 nm in the visible light range when an aqueous solution containing the dye and having a pH of 3 is prepared with a citrate buffer. Point to.
Here, since the color difference ΔE value is a numerical value that quantifies and shows the difference in color tone from the red radish dye in the Hunter Lab color system, the smaller the value, the brighter the red color tone closer to the red radish dye. It becomes a value and is suitable. Specifically, if the color difference ΔE value from the red radish dye at pH 3 is 10 or less, it is judged to be good. In particular, those having a pH of 8 or less, more preferably 5 or less, and even more preferably 3 or less are preferable.
Here, as the red radish dye used for comparison in the evaluation, a red radish dye having a maximum absorption wavelength of 513 nm in the visible light region can be used. A commercial item can be used as the red radish dye. As an example, a pigment preparation "Vegita Red [registered trademark] AD" manufactured by Saneigen FFI Co., Ltd. can be mentioned.

Technical Terms Related to Dyes The main terms related to dyes used herein are described below.
In the present specification, the “Hunter Lab color system (Lab system)” is a color system that forms a color solid composed of orthogonal coordinates consisting of a-axis and b-axis indicating chromaticity and an L-axis perpendicular thereto. Is.
Here, the "L value" is a value expressing the brightness numerically. When the L value = 100, it becomes white, and when the L value = 0, it becomes black. The "a value" is a value that numerically expresses the color tones of red and green. The higher the a value, the stronger the red color, and the lower the a value, the stronger the green color. The "b value" is a value that numerically expresses the color tones of yellow and blue. The higher the b value, the stronger the yellow color, and the lower the b value, the stronger the blue color.

In the present specification, the "CHROMA value" is a value obtained by numerically expressing the distance from the origin in the Hunter Lab color system by the following formula (1). It is used as a value indicating saturation. The larger the value is, the more vivid the color is.

In the present specification, the “color difference (ΔE)” is a point between (a ₁ , b ₁ , L ₁ ) and (a ₂ , b ₂ , L ₂ ) in which two colors are plotted in the Hunter Lab color system. It is a value expressed numerically by calculating the distance between the two by the following formula (2).

In the present specification, the "HUE value" refers to the formation angle of a straight line connecting the origin and the plot (a value, b value) on the orthogonal coordinates of the a-axis and the b-axis in the Hunter Lab color system, and the Munsell hue circle. It is a value representing the hue expressed by being converted into the hue notation in. It is a value representing the hue with a symbol and a numerical value.

In the present specification, the “maximum absorption wavelength” (λmax) refers to a light wavelength (nm) at which the dye or the dye composition has a maximum absorption in the visible light region. Further, in the present specification, the "absorbance" is a value indicating the degree to which a substance absorbs light. For example, the absorbance (A _λ ) of the maximum absorption wavelength (λmax) can be obtained by the following equation (3). In the formula, A means absorbance, λ means maximum absorption wavelength, A _λ means absorbance at maximum absorption wavelength, I means incident light intensity, and I ₀ means transmitted light intensity.

In the present specification, the "color values" means "color value ^E _{10% 1 cm",} the "color value ^E _{10% 1 cm",} when the preparation of the 10 weight% of the dye composition-containing solution, It is a value calculated based on the absorbance (A: Absorbance) at the maximum absorption wavelength (λmax) in the visible light region using a measurement cell having an optical path length of 1 cm.
In the present specification, "conversion of color value" refers to conversion of a dye (a dye composition) into a numerical value per color value. For example, 0.05% by mass in terms of a color number of 80 means that the amount of the dye contained in the solution is 0.05% by mass when the dye (dye composition) is adjusted to have a color number of 80. means.

In the present specification, the “citrate buffer solution (pH 3.0)” is a buffer solution adjusted to pH 3.0 with citric acid and a phosphate (Na ₂ HPO ₄ ), and specifically, It can be prepared according to the method described in the 9th edition Food Additives Official Standard (Ministry of Health, Labor and Welfare of Japan). Also known as McIlvaine buffer.

In the present specification, the "dye residual ratio" is a value calculated by the following formula (4) based on the absorbance of the maximum absorption wavelength of each dye measured before and after the test such as stability. In the present specification, the residual ratio of the dye compound in which the maximum absorption wavelength is maintained is calculated as the dye residual ratio, and is used as a value for evaluating the ratio of the dye compound in which the color development characteristics are stably maintained.

[Compositional characteristics relating to compound stability]
The anthocyanin-based pigment composition according to the present invention exhibits properties related to compound stability derived from the anthocyanin-based pigment composition of a sweet potato plant as a raw material.
In addition, about the suitable aspect and/or technical feature in embodiment shown below, it can be used with reference to the description of the above-mentioned paragraph regarding a sweet potato plant.

Compositional properties related to compound stability (acyllation modification rate, etc.)
The anthocyanin-based pigment composition according to the present invention has a compositional property containing a high content of an anthocyanin compound acylated with phenolic acids.
Here, examples of the phenolic acids for acylating and modifying the anthocyanin compound include hydroxycinnamic acids and hydroxybenzoic acids, and the anthocyanin-based dye composition according to the present invention, specifically, is acylated with hydroxycinnamic acids. It is characterized by a high content of the converted anthocyanin compound. On the other hand, the anthocyanin-based pigment composition according to the present invention is characterized in that the content of the anthocyanin compound acylated with hydroxybenzoic acids is low.

As the anthocyanin dye composition according to the present invention, those having compositional characteristics with a high modification rate of hydroxycinnamic acids are suitable.
That is, the sweet potato-derived anthocyanin-based pigment composition according to the present invention has the following characteristics:
As for the composition of the anthocyanin-based dye, the peak area ratio of the anthocyanin compound having a hydroxycaterous acid modifying group in the HPLC analysis shows 50% or more with respect to the peak area of all the anthocyanin compounds.
If the ratio is 50% or more, it is judged to be good from the viewpoint of compound stability. In particular, the ratio is 55% or more, more preferably 60% or more, still more preferably 65% or more, even more preferably 70% or more, particularly preferably 75% or more, even more preferably 80% or more, still more particularly preferably. Is preferably 85% or more.

Further, in the anthocyanin dye composition according to the present invention, the peak area ratio of the anthocyanin compounds having a hydroxybenzoic acid modifying group in HPLC analysis is less than 50% with respect to the peak areas of all the anthocyanin compounds.
If the ratio is less than 50%, it is judged to be good from the viewpoint of compound stability. In particular, the proportion is less than 45%, more preferably less than 40%, even more preferably less than 35%, even more preferably less than 30%, particularly preferably less than 25%, even more preferably less than 20%, even more particularly preferably Is preferably less than 15%.

Further, the sweet potato-derived anthocyanin dye composition according to the present invention has a peak area ratio of an anthocyanin compound having a caffeic acid modifying group as R ₂ in the structural formula (I) in HPLC analysis, relative to the peak areas of all anthocyanin compounds. 50% or more.
If the ratio is 50% or more, it is judged to be good from the viewpoint of compound stability. In particular, an anthocyanin-based dye composition having a ratio of 55% or more, more preferably 60% or more, further preferably 65% or more, still more preferably 70% or more, and particularly preferably 75% or more is suitable.

As the anthocyanin-based dye composition according to the present invention, it is preferable that the compositional characteristics regarding the acylation modification rate show the above ratio as the ratio to the total anthocyanin compounds. It is particularly preferable that the modification ratio of the hydroxycinnamic acids shows the above ratio. Further, as the anthocyanin-based dye composition according to the present invention, one having the same tendency as the above-mentioned compositional properties regarding the acylation modification rate is preferable regardless of the type of aglycone.

The above-mentioned ratio regarding the anthocyanin-based dye composition is a value indicated by a value obtained by HPLC analysis. Here, the measurement wavelength, method, conditions, and the like for performing the HPLC analysis can be carried out in the same manner as described in the paragraph relating to the sweet potato plant described above.

Absorbance characteristics related to compound stability (A _{320 nm} / A _{530 nm} value)
The anthocyanin-based dye composition according to the present invention is a composition showing a value relating to the absorbance, which reflects the above-mentioned compositional characteristics with respect to its compound stability.
That is, the sweet potato-derived anthocyanin-based pigment composition according to the present invention has the following characteristics:
When an aqueous solution containing the anthocyanin dye composition and having a pH of 3 with a citric acid buffer solution was prepared so that the color value E ^10% _{1 cm} value was 0.3 to 0.7, the A _{320 nm} / A _{530 nm} value was obtained. 3 or more.
Here, the A _320nm /A _530nm value is a value indicating the ratio of A _320nm and A _530nm . A _{320 nm} indicates the absorption wavelength of the structure related to hydroxycinnamic acids, and A ₅₃₀ nm indicates the absorption wavelength of the anthocyanin compound. _Therefore , it can be determined that the higher the value, the higher the content of the structure related to hydroxycinnamic acids. That is, the higher the value, the more suitable anthocyanin-based dye composition in terms of compound stability.
Specifically, if the A _{320 nm} / A _{530 nm} value is 3 or more, it is judged to be good. In particular, an anthocyanin-based dye composition having a value of 3.1 or more, preferably 3.2 or more, more preferably 3.3 or more, and even more preferably 3.4 or more at pH 3 is preferable.

The value of the absorbance of the anthocyanin-based dye composition according to the present invention is a value that reflects the compositional characteristics of containing a high amount of the anthocyanin compound having a hydroxycinnamon acid modifying group. Therefore, the anthocyanin-based dye composition is stable. Demonstrates high characteristics.
When this value is used as an index, the stability of the anthocyanin-based dye composition according to the present invention can be evaluated by a simple absorbance measurement operation.

[Evaluation of compound stability]
The anthocyanin-based pigment composition according to the present invention exhibits excellent compound stability due to the compositional properties of the anthocyanin compound of the sweet potato plant as a raw material. The anthocyanin dye composition according to the present invention exhibits excellent light resistance particularly to light irradiation.

Stability to light The anthocyanin-based dye composition according to the present invention is a dye composition having extremely high resistance to light irradiation, and has the property that the color forming properties are not easily lost under light irradiation conditions. The degree of light fastness can be evaluated as high light fastness as compared with ordinary purple sweet potato dyes and red radish dyes, which are recognized to have relatively high stability.

As the anthocyanin-based dye composition according to the present invention, those showing a high dye residual ratio in a light abuse test are preferable.
That is, the sweet potato-derived anthocyanin-based pigment composition according to the present invention preferably has the characteristics described below:
An aqueous solution containing the anthocyanin-based pigment composition having a color value E of ^{10% and a} _{1 cm} value of 80 equivalent of 0.03% by mass, adjusted to Brix 10 ° with fructose-glucose liquid sugar, and adjusted to pH 3.0 is white fluorescent. When stored at 20000 lux and 25 ° C for 2 days, the following formula (11) is satisfied:
Equation (11): LR ₁ / LR ₀ > 1
Here, "LR ₁ " indicates the residual ratio of the dye after the aqueous solution containing the target anthocyanin-based dye composition is stored under the above conditions by light irradiation. Further, in "LR ₀ ", the aqueous solution prepared in the same manner as above was subjected to light irradiation storage under the above conditions, except that a purple sweet potato dye was blended in place of the anthocyanin dye composition. The residual rate of the dye after that is shown.
The value on the right side of the formula is a value showing the dye stability in the light resistance test as a ratio with the value of a usual purple sweet potato dye. When the value is larger than 1, it is preferable because it shows that the light resistance is superior to that of a usual purple sweet potato dye. In particular, those having a value of 1.1 or more, preferably 1.2 or more are more preferable.

Further, as the anthocyanin-based dye composition according to the present invention, it is preferable to use one having a small change in color tone in the light abuse test as compared with that before the test.
That is, the sweet potato-derived anthocyanin-based pigment composition according to the present invention preferably has the characteristics described below:
An aqueous solution containing the anthocyanin-based pigment composition having a color value E of ^{10% and a} _{1 cm} value of 80 equivalent of 0.03% by mass, adjusted to Brix 10 ° with fructose-glucose liquid sugar, and adjusted to pH 3.0 is white fluorescent. The following formula (12) is satisfied when the lamp is stored at 20,000 lux and 25° C. for 2 days:
Formula (12): LE ₁ /LE ₀ <1
Here, “LE ₁ ”represents the ΔE value before and after the light irradiation storage under the above conditions was performed on the aqueous solution containing the anthocyanin dye composition. Further, in "LE ₀ ", the aqueous solution prepared in the same manner as above was subjected to light irradiation storage under the above conditions, except that a purple sweet potato dye was blended in place of the anthocyanin dye composition. The ΔE values before and after are shown.
The value on the right side of the equation is a value indicating the degree of color tone change in the light resistance test by the ratio with the value of a normal purple sweet potato dye. When the value is smaller than 1, it is preferable since it is a value showing that it is more excellent in light resistance than a usual purple sweet potato dye. Particularly, those having the value of 0.8 or less, preferably 0.6 or less are more suitable.

The above value shown by the anthocyanin dye composition according to the present invention is a value showing that the anthocyanin dye composition exhibits high light resistance to light irradiation, and an anthocyanin having a hydroxycinnamic acid-modifying group. It is recognized as a value that reflects the compositional characteristics of high content of the compound.
The anthocyanin-based dye composition according to the present invention is a dye composition that can be suitably used for coloring distribution products and display products that require resistance to long-term fluorescent light irradiation, etc., due to the characteristics relating to the compound stability. Recognized as a thing.

Stability against heat The anthocyanin-based dye composition according to the present invention is a dye composition having resistance to heat, and has a property that the color development property is not easily lost under high temperature storage conditions. The degree of heat resistance can be evaluated to be as high as or comparable to that of ordinary purple sweet potato dyes and red radish dyes, which are recognized to have relatively high stability.

As the anthocyanin-based dye composition according to the present invention, those showing a high dye residual ratio when a heat abuse test is carried out are preferable.
That is, the sweet potato-derived anthocyanin-based pigment composition according to the present invention preferably has the characteristics described below:
An aqueous solution containing the anthocyanin-based pigment composition having a color value E of ^{10% and a} _{1 cm} value of 80 equivalent of 0.03% by mass, adjusted to Brix 10 ° with fructose-glucose liquid sugar, and adjusted to pH 3.0 in a dark place. And when stored at 50 ° C. for 7 days, the following formula (13) is satisfied:
Formula (13): HR ₁ /HR ₀ >0.9
Here, “HR ₁ ”indicates a dye residual ratio after high temperature storage under the above conditions for an aqueous solution containing the anthocyanin dye composition of interest. Further, "HR ₀ " is prepared after being stored at a high temperature under the above conditions in an aqueous solution prepared in the same manner as described above, except that a purple sweet potato dye is blended in place of the anthocyanin dye composition. Shows the residual rate of dye.
The value on the right side of the formula is a value showing the dye stability in the heat resistance test as a ratio with the value of a usual purple sweet potato dye. If the value is larger than 0.9, it is preferable because it shows a heat resistance comparable to or comparable to that of a normal purple sweet potato dye. In particular, it is more preferable that the value is 1 or more.

Further, as the anthocyanin-based dye composition according to the present invention, one having a small change in color tone as compared with that before the test when the heat abuse test is performed is preferable.
That is, the sweet potato-derived anthocyanin-based pigment composition according to the present invention preferably has the characteristics described below:
An aqueous solution containing the anthocyanin dye composition having a color value E of ^10% and 0.03 mass% in terms of _{1 cm} value of 80, adjusted to Brix 10° with fructose corn syrup and adjusted to pH 3.0 was placed in a dark place. And when stored at 50° C. for 7 days, the following formula (14) is satisfied:
Formula (14): HE ₁ /HE ₀ <1.1
Here, "HE ₁ " indicates the ΔE value before and after the aqueous solution containing the anthocyanin-based dye composition was stored at a high temperature under the above conditions. In addition, “HE ₀ ”is before and after performing high temperature storage under the above conditions on an aqueous solution prepared in the same manner as described above, except that a purple sweet potato dye is blended in place of the anthocyanin dye composition. Shows the ΔE value at.
The value on the right side of the equation is a value indicating the degree of color change in the heat resistance test as a ratio to the value of a normal purple sweet potato dye. When the value is less than 1.1, it is suitable because it has a heat resistance comparable to or comparable to that of a normal purple sweet potato dye. In particular, it is more preferable that the value is 1 or less.

The above values shown by the anthocyanin-based dye composition according to the present invention are recognized as values indicating that the anthocyanin-based dye composition exhibits heat resistance to high-temperature storage.
The anthocyanin-based dye composition according to the present invention is a dye composition that can be suitably used for coloring distribution products, display products, etc., which are required to be resistant to high temperature storage for a long time due to the characteristics related to the compound stability. Is recognized. It is also recognized that it can be used in modes that assume heat treatment such as retort sterilization, processing, and cooking.

[Other characteristics]
It is preferable that the anthocyanin-based dye composition according to the present invention has excellent properties in addition to the above-mentioned properties.

Odor Properties The anthocyanin dye composition according to the present invention exhibits color development properties close to the color tone of red radish dyes, and has good odor properties with respect to odor properties similar to ordinary sweet potato dyes.

Absorption property when ingested as food or drink When the anthocyanin-based pigment composition according to the present invention is ingested as food or drink, it is preferable that the anthocyanin-based dye composition has excellent intestinal absorption properties than conventional purple sweet potato dyes. is there.
That is, the sweet potato-derived anthocyanin-based pigment composition according to the present invention preferably has the characteristics described below:
When an aqueous solution containing the anthocyanin dye composition and having a pH of 6.8 ± 2 was prepared with Hanks' Balanced Salt Solution buffer (HBSS buffer) so that the color value E ^10% _{1 cm} value was 5.0. In the permeability test of the intestinal absorption model using the Caco-2 cell sheet, the permeation amount of the anthocyanin compound 6 hours after the start of the test is 1.5 times or more the permeation amount of the purple sweet potato pigment.
In the present invention, it is preferable that the value is 1.5 or more. Those showing 2 or more, more preferably 2.5 or more, still more preferably 3 or more are particularly preferable.

[Method for producing dye composition]
The anthocyanin-based pigment composition according to the present invention can be produced from the plant body of the sweet potato plant described in the above paragraph as a raw material.
That is, the present invention includes an invention relating to a method for producing a sweet potato-derived anthocyanin pigment composition, which comprises using the above-mentioned plant body of a sweet potato plant as a raw material. Here, the plant body of the sweet potato plant includes the plant body of the sweet potato plant obtained by the above-described production method.
In the method for producing a dye composition according to the present invention, it is possible to produce the dye composition by the steps described below, but each step other than the characteristics relating to the raw material plant has the technical features according to the present invention. It is not limited to the steps described below as long as it does not substantially interfere with the effects. Further, the technical scope according to the present invention is not limited to the embodiment including all of the following steps.

Raw Material The anthocyanin dye composition according to the present invention can be produced using the plant body of the sweet potato plant described in the above paragraph as a raw material.
The plant used as a raw material for the method for producing the pigment composition according to the present invention may be the whole or a part of the plant as long as it contains an anthocyanin pigment, and any part may be used. it can. For example, any part of plant tissue such as tuberous root, root, leaf, petiole, vine, stem and flower can be used. In consideration of raw material yield and pigment yield efficiency, it is preferable to use tuberous roots, particularly matured and developed tuberous roots.
That is, as the raw material, a part or all of tubers or a part of the plant body containing tubers can be preferably used. In particular, a part or all of the tuberous root can be preferably used as the raw material.

Aspect in Extract In the method for producing a pigment composition according to the present invention, the pigment composition can be produced by obtaining an extract from a raw material plant.
When the pigment composition according to the present invention is prepared in the form of an extract, an anthocyanin compound is extracted from a raw material plant. As the extraction method, any known or unknown method can be used, but it is preferable to adopt a method with a good extraction yield of the anthocyanin compound. In addition, as a raw material when performing the extraction, it is possible to use the residue after extraction or the juice residue.

As a mode for performing the extraction operation from the raw material plant, it is preferable to use a raw material obtained by cutting, shredding, slicing, crushing, crushing, crushing, powdering or the like.
As the extraction solvent, any solvent that can be used for extracting the anthocyanin-based dye can be used, and for example, water, hydrous alcohol, alcohol or the like is preferably used. When a hydrous alcohol is used, an alcohol content of about 1 to 80% (v / v), preferably about 1 to 50% (v / v) is preferable. Examples of the hydrous alcohol or the type of alcohol that can be adopted as the alcohol include lower alcohols having 1 to 4 carbon atoms, and preferably ethanol.
It is also preferable to use water as the extraction solvent. Purified water, distilled water, ultrapure water, etc. are preferably used as the water, but any grade water used for food and drink production, pigment preparation, etc. can be used as the solvent without any problem. it can. As the solvent, it is also possible to use an aqueous solution containing various salts, organic acids, pH adjusters, pH buffers, lower alcohols and the like.
The amount of the solvent used with respect to the raw material is not particularly limited, but for example, 0.1 to 1000 parts by mass of the solvent with respect to 1 part by mass of the raw material, preferably 0.5 to 100 parts by mass with respect to 1 part by mass of the raw material. Can be used.
The extraction solvent is preferably an acidic solution. Specifically, it is preferable to use an acidic solution adjusted to pH 1 to 6, preferably pH 1 to 5, and more preferably pH 1 to 4. As a means for adjusting the extraction solvent to be acidic, a conventional method using an inorganic acid and/or an organic acid can be used.
The extraction conditions are not particularly limited, but for example, at normal pressure, 1 to 100 ° C. can be mentioned, and preferably about 10 to 90 ° C. can be adopted. The extraction time can be appropriately determined in consideration of conditions such as temperature. For example, it is about 1 minute to several days, but is not particularly limited thereto.

Aspects for juices, pastes, powders, etc. Further, in the method for producing a pigment composition according to the present invention, it is also possible to produce a pigment composition without going through an extraction step.
When the pigment composition according to the present invention is prepared as a form of a processed plant product that does not undergo an extraction step, the mode of the primary processed product of a sweet potato plant described in the above paragraph can be used as it is. For example, the juice of the plant (including juice, pressed liquid, semi-solid, solid, etc.), the primary processed product of the plant itself (cut, ground, paste, puree, etc. of the plant). Dried products, powdered products, heat-treated products, frozen-treated products, various physical treatments, various chemical treatments, various enzyme treatments, etc.) can be used as the dye composition. Further, in this aspect, the residue after extraction and the squeezed residue can be used as the dye composition as long as the conditions such as color development characteristics are satisfied.
In this embodiment, various treatment steps such as squeezing, pasting, and pulverizing can be performed by any known or unknown method, but a method having a good extraction yield of the anthocyanin compound should be adopted. Is preferred. In addition, various processing conditions and the like can be appropriately determined in consideration of extraction efficiency.

In the method for producing a dye composition of the present invention such as various treatments, a purification treatment can be carried out depending on the intended use to obtain a dye composition of desired quality and form. In particular, in the production of dye preparations and dye compositions in which deodorization is important, it is preferable to carry out this step.
The purification treatment and/or the deodorization treatment can be performed using a conventional method. For example, an embodiment in which an adsorption treatment using a synthetic resin or the like, an ion exchange treatment, a membrane separation treatment or the like is performed can be mentioned.
In addition, as a deodorizing treatment separate from the purification treatment, it is also possible to perform an enzyme treatment such as protease, a microbial treatment, or the like depending on the intended use.

In the method for producing an anthocyanin pigment composition according to the present invention, a plant extract obtained by the above operation, a processed plant product prepared without an extraction operation, and the like are subjected to solid-liquid separation and purification treatment according to the intended use. , Concentration treatment, dilution treatment, pH adjustment, drying treatment, sterilization treatment and the like can be performed to obtain an anthocyanin pigment composition having a desired quality and / or form.
These steps can be performed by combining desired processes, and the desired processes can be performed a plurality of times.
Examples of the anthocyanin-based pigment composition according to the present invention include various forms such as liquid, paste, gel, semi-solid, solid, and powder, but are particularly limited to these forms. is not.
Further, the anthocyanin-based dye composition according to the present invention may be in a mode in which other functional components and the like are blended as long as the color development characteristics, compound stability and the like are not substantially impaired. For example, antioxidants, pH adjusters, thickening polysaccharides, other food materials and the like can be blended, but the present invention is not particularly limited thereto. Further, it is also possible to obtain a dye composition having a desired color tone and properties by mixing a dye composition or a dye preparation different from the anthocyanin-based dye composition according to the present invention.

3. Uses The present invention includes inventions relating to dye preparations, foods and drinks, cosmetics, pharmaceuticals, quasi drugs, daily necessities for hygiene, or feeds containing the above-described anthocyanin dye composition.
Further, in the present invention, which is characterized by including the step of using the above-mentioned anthocyanin-based pigment composition as a raw material, pigment preparation, food and drink, cosmetics, pharmaceuticals, quasi-drugs, daily necessities for hygiene, or feed. An invention relating to a manufacturing method is included.
Here, the anthocyanin-based dye composition includes the anthocyanin-based dye composition obtained by the method for producing an anthocyanin-based dye composition described in the above paragraph.

[Dye preparation]
As a usage form of the anthocyanin-based dye composition according to the present invention, it is particularly preferable to use it as a form of a dye preparation.
Examples of the form of the pigment preparation according to the present invention include liquid, paste, gel, semi-solid, solid, powder and the like, and are not particularly limited. In addition, processed solid shapes such as granules and tablets can be mentioned.
Further, since the anthocyanin-based pigment composition according to the present invention is water-soluble, it can be used as it is as a water-soluble pigment preparation, but it is an oil-soluble pigment preparation (W / O type) or a double emulsified dye preparation. It is also possible to process it into (W / O / W type) or the like and use it.

In the dye preparation according to the present invention, the amount or content of the anthocyanin-based dye composition can be appropriately adjusted depending on the type and purpose of the dye preparation and is not particularly limited, but, for example, the color value E ^10% It is preferable that the composition be blended so that the _{1 cm} value is 20 or more, preferably 30 or more, and more preferably 40 or more. The upper limit of the color value of the dye preparation according to the present invention is not particularly limited, but for example, the color value E ^10% _{1 cm} value 800 can be mentioned.
The blending amount or content of the anthocyanin-based dye composition in the dye preparation may be calculated on the basis of the above-mentioned color value, and the mass base is, for example, 0.1 to 99% by mass, preferably 1 to 90% by mass, and further Preferably, it is 5 to 75% by mass.

In the dye preparation according to the present invention, other functional ingredients and the like can be blended as long as the color development characteristics and compound stability of the anthocyanin-based dye composition according to the present invention are not substantially impaired. is there. For example, antioxidants, pH adjusters, thickening polysaccharides, flavor compounds, other food materials and the like can be blended, but the present invention is not particularly limited thereto.

The present invention includes an invention relating to a method for producing a dye preparation, which comprises blending or containing the above-mentioned anthocyanin dye composition. That is, the present invention includes an invention relating to a method for producing a dye preparation, which comprises using the anthocyanin dye composition according to the present invention.
Regarding the compounding amount or content of the anthocyanin-based dye composition in the production method, it is possible to use with reference to the description in the above paragraph. The manufacturing process in the manufacturing method can be used with reference to the description in the above paragraph. Moreover, it is also possible to adopt a conventional method except that the above-mentioned anthocyanin dye composition is used.
The dye preparation produced here shows a product form as a dye preparation (or a dye agent) containing the anthocyanin compound according to the present invention described above, and the compound composition is a composition containing an anthocyanin-based dye. It can also be regarded as one form.

[Various products]
The red pigment composition or pigment preparation according to the present invention can be suitably used as a natural colorant for products such as food and drink, cosmetics, pharmaceuticals, quasi drugs, daily necessities for hygiene, or feed. Is. That is, in the present invention, it is possible to provide food and drink, perfume, cosmetics, pharmaceuticals, quasi drugs, daily necessities for hygiene, or feed containing the red pigment composition or pigment preparation according to the present invention.
Here, an example of a product that can be colored with the red dye composition or dye preparation according to the present invention is shown below, but the colorable product according to the present invention is not limited thereto.
Examples of the "food and drink" include beverages, frozen desserts, desserts, sugar confectionery (for example, candy, gummy, marshmallow), gum, chocolate, confectionery (for example, cookies), bread, processed agricultural products (for example, pickles, etc.). ), processed meat products, processed marine products, dairy products, noodles, seasonings, jellies, syrups, jams, sauces, liquors and the like.
Examples of the "cosmetics" include skin lotions, lipsticks, sunscreen cosmetics, makeup cosmetics, and the like.
Examples of the “pharmaceutical product” include various tablets, capsules, drinks, troches, mouthwashes, and the like.
Examples of the "quasi drug" include nutritional supplements, various supplements, toothpaste, mouth freshener, odor preventive agent, hair restorer, hair restorer, skin moisturizer, and the like.
Examples of “hygiene products” include soaps, detergents, shampoos, rinses, hair treatments, toothpastes, bath salts, and the like.
Examples of the "feed" include various pet foods such as cat food and dog food, feed for ornamental fish and cultured fish, and the like.

The dye composition or dye preparation according to the present invention has excellent characteristics of bright red color development, and has the same color development characteristics as red radish dye, especially in an acidic region. Therefore, the dye composition or dye preparation according to the present invention can be preferably used for coloring a product in which at least a part or all of the pH is acidic. Here, as acidic conditions, a pH of less than 7 can be mentioned, preferably a pH of 5 or less, and more preferably a pH of 4 or less.
The dye composition or dye preparation according to the present invention exhibits a color tone close to that of the red radish dye in the pH range even when the pH is weakly acidic to alkaline, and it can be used by assuming that color tone. That is, the above description regarding pH describes a preferable usage mode, and does not mean that the usage mode of the dye composition or dye preparation according to the present invention is limited to use in an acidic range.

Since the dye composition or dye preparation according to the present invention has excellent light resistance, it can be suitably used for coloring products and the like that are premised on light exposure during storage and display. is there. Further, since the dye composition or dye preparation according to the present invention has heat resistance, it can also be used for products that are expected to be exposed to high temperature storage.

The dye composition or dye preparation according to the present invention can be suitably used for coloring purposes in the production process of the above products.
The step of coloring the above-mentioned product can be carried out according to a conventional method for each product, except that the dye composition or the dye preparation according to the present invention is blended or contained as a natural dye material.
The blending amount or content of the dye composition or the dye preparation according to the present invention with respect to these products can be appropriately adjusted according to the type and purpose of the product. For example, the content of the dye composition or the dye preparation in the product is 0.001 to 1% by mass, preferably 0.005 to 0.5% by mass, and more preferably 0.01 to 0.2, in terms of color value of 80. It is possible to mix or contain it so as to be mass%, more preferably 0.02 to 0.1 mass%.

The present invention includes an invention relating to a method for producing various products, which comprises blending or containing the above-mentioned anthocyanin-based pigment composition or pigment preparation. That is, in the present invention, characterized by using the anthocyanin pigment composition or pigment preparation according to the present invention, a method for producing food and drink, perfume, cosmetics, pharmaceuticals, quasi drugs, daily necessities for hygiene, or feed, etc. Includes inventions related to.
The blending amount or content of the anthocyanin-based dye composition or the dye preparation in the production method can be used with reference to the description in the above paragraph. In addition, as the production process in each production method, conventional methods in various technical fields can be adopted except that the above-mentioned anthocyanin-based pigment composition or pigment preparation is used.
The various products produced here show product forms containing the above-described anthocyanin compound according to the present invention, but the compound composition can also be regarded as one form of a composition containing an anthocyanin dye. is there.

Hereinafter, the present invention will be described with reference to examples, but the scope of the present invention is not limited thereto. The symbol "ND" in the table below indicates that the signal peak is below the detection limit.

[Example 1] "Production of sweet potato line containing a high amount of pelargonidin anthocyanin"
A new sweet potato line with a high content of pelargonidin anthocyanins was created from the progeny population of a specific sweet potato line. The outline of the production of the system is shown in FIG.

(1) "Creation of a new sweet potato strain"
As the original varieties, the breeding line 89360-8 (line with early hypertrophy of clump roots, FERM BP-22365), which is the breeding line owned by the present inventors, is used as the seed parent, and the nine line 294 (high anthocyanin pigment content). Crosses were carried out using the high color value line, FERM BP-22364) as the pollen parent. As a result, in the offspring population, about 10% of the individuals in which tuberous roots differed from the usual sweet potato lines exhibited a red color tone, and a plurality of individuals appeared.
Here, the original varieties, 9-series 89360-8 and 9-series 294, are both lines having a normal purple-colored lump root, and an anthocyanin pigment exhibiting a red tone due to the combination of the genetic backgrounds of these original varieties. It was recognized that the strains of were appearing independently.

Among the individuals exhibiting the red color tone, individuals having particularly vivid red color were selected, and a new bright red strain, 9 strain 06353-19, was created from the self-population (FIG. 8). This line was used as a seed parent, and a breeding line for improving the trait of lump root hypertrophy was bred as a pollen parent, and individuals having good red color development characteristics and good traits related to lump root hypertrophy were selected from the offspring. A private group was obtained from the selected individuals and a new sweet potato line, Kyushu 187, was created (Fig. 8).
Kyushu No. 125 (FERM BP-22363) is used as the trait donor parent for lump root hypertrophy, but the strain does not contain anthocyanin itself, and the trait containing a high amount of pelargonidin-based anthocyanin according to the present invention. It is a system that has nothing to do with.

(2) "Preparation of extract"
50 g of the roots of Kyushu No. 187, which is the above production line, was cut into square pieces with a kitchen knife, immersed in 200 g of 0.5% sulfuric acid water, and allowed to stand overnight at room temperature for extraction treatment. This was squeezed with a net, and suction filtration was performed using a filter paper (NO.5 filter, manufactured by Advantech Toyo Co., Ltd.) to recover the filtrate.

(3) "HPLC / MS analysis"
The filtrate obtained above was subjected to HPLC/MS analysis as an extract from tuber root of Kyushu 187. The said HPLC analysis was performed with the apparatus and conditions shown below. The structure of each peak of the obtained chromatogram was estimated from the precise mass by MS analysis, and the peak area ratio indicating each anthocyanin compound was calculated. The obtained chromatogram is shown in FIG. The table below shows the results of structural estimation and calculated peak area ratio.
Here, in the table below, each peak number indicating the pelargonidin-based anthocyanin compound indicates the number corresponding to each peak indicating the pelargonidin-based anthocyanin in the above chromatogram. Further, each peak number of cyanidin-based anthocyanins and peonidin-based anthocyanins is shown as a modified number in which the peak number of the corresponding modification pattern in pelargonidin-based anthocyanins is added with “′” or “″”. Further, “R ₁ ”to “R ₃ ”in the table represent functional groups in the structural formula (I), “H” is a hydrogen atom, “OH” is a hydroxyl group, and “OCH ₃ ” is a methoxy group. , "PHB" indicates a p-hydroxybenzoic acid modifying group, "Caf" indicates a caffeic acid modifying group, and "Fer" indicates a ferulic acid modifying group.

[Devices and conditions used for HPLC / MS]
Equipment: UltraMate 3000 HPLC systems
Q Active Hybrid Quadrupole-Orbitrap Mass Spectrometer (manufactured by Thermo Fisher Scientific)
Column: L-column ODS (2.1 x 150 mm, 3 μm)
(Manufactured by Chemical Substance Evaluation Research Organization)
Mobile phase: 0.1% formic acid water/0.1% formic acid-containing acetonitrile Flow rate: 0.2 mL/min Temperature: 40°C
Measurement wavelength: 530 nm

[Gradient condition]
(Time) (0.1% formic acid water) (0.1% formic acid-containing acetonitrile)
0 minutes: 95% 5%
15 minutes: 82% 18%
40 minutes: 30% 70%
42 minutes: 30% 70%

(4) "Various analyzes of anthocyanin pigment composition"
Using the measurement data of the HPLC / MS analysis obtained above, the compositional characteristics of the anthocyanin dye in the Kyushu No. 187 mass root extract were analyzed.

Characteristics relating to aglycone In the measurement data of the above HPLC/MS analysis, an analysis focusing on the difference in the type of aglycone was performed. As a result of calculating and comparing the total peak area value of each peak area ratio of aglycone anthocyanidin, about 85% of the peak area of all anthocyanin compounds detected from the mass root extract of Kyushu No. 187 is pelargonidin-based anthocyanin. It was shown to be the peak area derived from the compound (Table 6).

From the above results, it was shown that Kyushu No. 187, which is a newly produced line, is a sweet potato line which has completely different compositional characteristics from the ordinary sweet potato variety line in the characteristics relating to the basic structure of the anthocyanin pigment.
In view of these results, Kyushu No. 187 was recognized to be a sweet potato strain in which the production and accumulation of cyanidin and peonidin were suppressed and the metabolic function that enables the production and accumulation of pelargonidin was acquired.

Characteristics Related to Acylation Modification Rate In the measurement data of the above-mentioned HPLC/MS analysis, focusing on the acylation modification of anthocyanin, the sum of each peak area of the compound containing a specific modification group at the acylation modification site was calculated, and the total peak area was calculated. The ratio with and was calculated.
As a result, in the anthocyanin pigment from the tuberous root extract of Kyushu 187, about 91.5% of the peak area of all anthocyanin compounds was an acylated anthocyanin having a phenolic acid modifying group (Table 7). Moreover, as a mode of acylation modification with a phenolic acid in the anthocyanin dye, the ratio of the peak areas of compounds containing a modifying group of hydroxybenzoic acids is as low as 23.6% of the peak areas of all anthocyanin compounds. On the other hand, on the other hand, the ratio of the peak areas of the compounds containing the modifying group of hydroxycinnamic acids, which acts to improve the stability, was 87.2% of the peak areas of all the anthocyanin compounds, which was a very high value.
In addition, the ratio of the acylation modification mode showed the same tendency even with the ratio value calculated for each series of compounds having each aglycone as a basic skeleton. In particular, regarding the modification ratio of hydroxycinnamic acid, a value in the range of 86 to 90.1% was shown even when the skeleton structure of aglycone was different, and it was shown that the ratio was substantially the same as the ratio to all anthocyanin compounds. (Table 7).

From the above results, Kyushu 187, which is a newly produced strain, contains a high content of anthocyanin compounds acylated and modified with phenolic acid, and most of them (about 87.2% of all anthocyanins, phenolic acid-modified anthocyanins) are contained. Approximately 95.3% of them) were shown to be of the sweet potato strain, which is an anthocyanin compound containing a modifying group of hydroxyphenolic acids. Since the hydroxycinnamic acids act to improve the stability of the anthocyanin compound, it was confirmed that the anthocyanin dye from Kyushu No. 187 is a dye composition containing a highly stable anthocyanin compound at a high content.
Further, the modification mode of the acylation showed the same tendency even in the compounds between the skeleton structures having different aglycones, and in particular, regarding the modification ratio of hydroxycinnamic acids, no difference in the skeleton structures having different aglycones was substantially observed.
These results suggest that Kyushu No. 187 is a sweet potato strain that has a weak metabolic activity that controls acylation modification with hydroxybenzoic acid, while a significantly strong metabolic activity that controls acylation modification with hydroxycinnamon acids. It was a result.

Characteristics of Caffeic Acid Modification Rate of Specific Modification Site In the measurement data of the above HPLC/MS analysis, each modification mode defined by the combination of R ₂ and R ₃ which is the acylation modification site of the general structural formula (I) of anthocyanin The ratio of the peak area showing the compounds of the modification modes A to H detected in Tables 3 to 5 to the total peak area was calculated (Table 8).
As a result, it was shown that the anthocyanin pigment from the tuberous root extract of Kyushu 187 has a compositional characteristic that it contains a large amount of anthocyanin compounds having the structures represented by modification modes E, G, and H. Here, in the structure shown modified style E, G, with H, was structure common in terms acylation modification of the R ₂ is a caffeic acid modifying group.

Therefore, to calculate the sum of the peak areas of anthocyanin compounds with caffeic acid modifying group as R _2, and calculates the ratio between the total peak area. In addition, the total peak area of the anthocyanin compounds in which R ₂ is not a caffeic acid modifying group was calculated in the same manner, and the ratio to the total peak area was calculated (Table 9).
As a result, the total peak area of the anthocyanin compounds having a caffeic acid modifying group as R ₂ accounted for 80.4% of the peak areas of all the anthocyanin compounds. Regarding the ratio of the modification mode, the ratio value calculated for each series of compounds having each aglycone as a basic skeleton also showed the same tendency result, and it was recognized as a value close to the ratio for all anthocyanin compounds.
On the other hand, the total peak area of the anthocyanin compounds having no caffeic acid modifying group as R ₂ was only 19.6% of the peak areas of all the anthocyanin compounds.

From the above results, in Kyushu No. 187, which is a newly produced strain, R ₂ which is the acylation modification site of the general structural formula (I) of anthocyanin has a high content of anthocyanin compound acylated with caffeic acid (80 It was shown to be a sweet potato strain (% or more). Since caffeic acid is a kind of hydroxycinnamic acids that acts to improve the stability of anthocyanin compounds, the anthocyanin pigment from Kyushu 187 is recognized as a pigment composition containing a high content of anthocyanin compounds with excellent compound stability. Was done. In addition, the modification mode of caffeic acid at the R ₂ site showed the same tendency even in compounds having skeleton structures different in aglycone.
These results indicate that Kyushu 187 is a sweet potato line having a strong metabolic activity in which R ₂ of the general structural formula (I) of anthocyanin is acylated and modified with caffeic acid.

(5) "Reference to deposited biomaterials"
Ipomoea batatas 9 series 89360-8, 9 series 294, and Kyushu 125, which are sweet potatoes used in the above production process, were released on April 25, 2018 by the Product Evaluation Technology Infrastructure Organization. A deposit application was made to the Patent Organism Depositary, and the deposit number was given to the 9th series 89360-8 with "FERM P-22365", the 9th series 294 with "FERM P-22364", and Kyushu 125 with "FERM P-22363". It was.
Regarding these, a request for transfer to the international deposit was made to the same organization on November 19, 2018, "FERM BP-22365" for 9 series 89360-8, "FERM BP-22364" for 9 series 294, and Kyushu 125. The international deposit number of “FERM BP-22363” has been assigned to. In addition, regarding these biological materials, a deposit certificate regarding the original deposit and a certificate regarding survival were issued on January 21, 2019.

[Name and address of depository organization (international depositary authority)]
Name: National Institute of Technology and Evaluation Patent Organism Deposit Center Address: 292-0818 Room 2-5-8 Kazusakamatari, Kisarazu City, Chiba Prefecture [Date of deposit]
Domestic Deposit Date (Domestic Deposit Date): April 25, 2018 Notification Date of Domestic Deposit: July 6, 2018 Request Date for Transfer to International Deposit: November 19, 2018 Original Deposit issued by International Depositary Authority Issuance date of consignment certificate:
January 21, 2019 Certificate of Survival Issued by International Depositary Authority Date:
January 21, 2019 [Accession number]
FERM BP-22365: Ipomoea batatas (L.) Lam. 9 series 89360-8
FERM BP-22364: Ipomoea batatas (L.) Lam. 9 series 294
FERM BP-22363: Ipomoea batatas (L.) Lam. Kyushu No. 125 [Deposit]
Name or name: National Research and Development Corporation Agriculture and Food Industry Technology Research Organization Chairman Address: Postal code 305-8517 3-1-1 Kannondai, Tsukuba City, Ibaraki Prefecture
[Mention on deposit]
The Depositary authorizes the Applicant to refer to the depositary organism in this application. In addition, the depositary has given the applicant consent to make the deposited organism available to the public.
[Characteristics of deposited organisms]
i) The characteristics of each deposited organism with respect to FERM BP-22365, FERM BP-22364, and FERM BP-22363 are described in 1. in the embodiment of the invention according to the present specification. Paragraphs of new sweet potato plants (particularly, paragraphs of original varieties, hybrid or progeny groups of original varieties, production methods, etc.), and examples according to the present specification (particularly, Examples 1, 13 to 15, etc.), As described in.
ii) Also, information on the taxonomic location and scientific properties common to FERM BP-22365, FERM BP-22364, and FERM BP-22363 is as follows.
.."Taxonomy position"
Sweet potato species (Ipomoea batatas (L.) Lam.)
.."Information on scientific properties"
The genome is homoploid. The aerial part is mainly composed of a stem, a petiole, and a leaf blade, and the petiole is a 2/5 alternans leaflet, and branching occurs from the main stem. Adventitious roots extending from the roots of the nodules grow and form tuberous roots, which are starch storage organs. Flower buds differentiate under short-day conditions and reach flowering at constant temperatures.

(6) "Summary"
By selecting the mating population obtained from the mating of the original varieties, 9-series 89360-8 and 9-series 294, individuals with red-colored clump roots different from the normal sweet potato strain appear independently at a certain frequency. Was shown. It was shown that the newly produced line in which the tuber root has a reddish tone in this example is a new red sweet potato line having a high content of pelargonidin anthocyanin as a main pigment.
Here, the original varieties used in the present example are all lines having a normal purple-colored tuber root, because it is not a tuber tube line exhibiting a bright red color tone, the genetic background of these original varieties By the combination, it was confirmed that a line with a high content of pelargonidin-based anthocyanin pigment, which does not usually appear, appeared.
The production line according to the present invention is a sweet potato line having a genetic background in which the metabolic activity governing the acylation modification with hydroxycaterous acids is remarkably strong with respect to the synthetic pathway of the anthocyanin compound, and in particular, the R of the general structural formula (I) of anthocyanin. _It was confirmed to be a sweet potato line having a strong genetic background in which ₂ is acylated with caffeic acid and has a strong metabolic activity.
In this example, Kyushu No. 187, which is a strain with further improved clot root trait, was created from one of the newly produced strains.

[Example 2] "Preparation of anthocyanin-based pigment composition (pigment preparation)"
A pigment preparation, which is an anthocyanin-based pigment composition, was prepared using the mass roots of a novel sweet potato strain having excellent bright red color development produced in the above example as a raw material.

The lump root of Kyushu No. 187, which was the above-mentioned production system, was finely chopped with a food processor and immersed in 0.3% sulfuric acid water, which was twice the weight of the raw material. Then, the extraction operation was performed by stirring with a stirrer for 1 hour and leaving still overnight.
This was squeezed with a net and passed through 50 mesh to remove the residue, and the pH of the obtained filtrate was adjusted to pH 2.5 ± 0.2 with sodium hydroxide. The mixture was heated to 90° C., naturally cooled to 40° C., allowed to stand overnight, and centrifuged at 10,000 rpm for 10 minutes to collect a supernatant.
The obtained supernatant was suction-filtered using a filter paper (NO.2 filter, φ150 mm, manufactured by Advantech Toyo Co., Ltd.) on which diatomaceous earth had been formed in advance to collect the filtrate.

The obtained filtrate is passed through a column filled with a synthetic adsorption resin for adsorption, washed with tap water, and then desorbed the dye component from the adsorption resin with a 50% (v/v) ethanol aqueous solution containing citric acid. I let you.
After concentrating the recovered solution under reduced pressure, a concentrated solution was prepared so that the color value E was ^{10%, the} _{1 cm} value was 80, and the ethanol concentration was 20% (v / v). This was heat-sterilized at a temperature of 70° C. and divided into small portions to prepare a pigment preparation (Sample 2-1).
Further, a purple sweet potato pigment preparation (Sample 2-2) was prepared in the same manner as above, except that the tuber roots of the variety “Ayamurasaki” were used as a comparative sample.

[Example 3] "Evaluation of coloring characteristics of dye composition"
The color development characteristics of the anthocyanin pigment composition derived from the red sweet potato strain prepared in the above Examples were evaluated.

(1) "Evaluation of color development characteristics"
For the red sweet potato pigment prepared in Example 2, 0.3% citric acid water was used to prepare a test solution having a color value of E ^10% _{1 cm} 80 of 0.1% by mass, and the spectrophotometer (V -560, manufactured by JASCO Corporation, optical path length 1 cm of the measurement cell) was used to perform transmitted light color measurement at a measurement wavelength of 380 to 780 nm, and the three stimulus values (L value, a value, and b) of the Hunter Lab color system were performed. Value) was measured.
The measured values were used to evaluate lightness, saturation, and hue. As the "brightness", the measured L value was used as it was for evaluation. The “saturation” was evaluated by calculating the CHROMA value using the above formula (1). "Hue" was evaluated by calculating a HUE value. Further, as a value for quantitatively evaluating the difference in color development characteristics from the control sample (red radish dye), the "ΔE value" was calculated and evaluated using the above formula (2).
The maximum absorption wavelength (λmax) was calculated by measuring the absorbance of the maximum absorption portion having a wavelength of 500 to 545 nm using a citrate buffer (pH 3.0) as a solvent.
As a comparative test, a test solution was prepared and tested in the same manner as above using the purple sweet potato dye prepared in Example 2. Further, as a control test, a commercially available red radish dye (“Vegeta Red [registered trademark] AD” manufactured by San-Ei Gen FFI Co., Ltd.) was used to prepare a test solution in the same manner as above, and the test was conducted. (Table 10).

As a result, the red sweet potato dye (Sample 3-1) produced in the above-mentioned examples showed values extremely close to those of the red radish dye (Sample 3-3) in all measurement items, and the color difference ΔE value was only 0. It was 93. Further, the HUE value of the red sweet potato dye (Sample 3-1) was 4.6R (color tone close to red in JIS hue), which was completely the same as that of the red radish dye (Sample 3-3). The maximum absorption wavelength of the red sweet potato dye (Sample 3-1) was 512 nm, which was very similar to the red radish dye (Sample 3-3) of 513 nm. It was also confirmed in terms of absorption wavelength. Further, even when the filling reagent bottle was visually observed, the red sweet potato pigment (Sample 3-1) exhibited a bright and vivid red color tone.
On the other hand, the usual purple sweet potato dye (Sample 3-2) derived from Ayamurasaki shows different values from the red radish dye (Sample 3-3) in all measurement items, and the color difference ΔE value is about 28, which is a large gap. showed that. Also, the HUE value was 8.4 RP (intermediate color tone of purplish red to purplish red in JIS hue), which was completely different from that of the red radish dye (Sample 3-3). In addition, the maximum absorption wavelength of the purple sweet potato dye (Sample 3-2) is 528 nm, which is significantly different from the 513 nm of the red radish dye (Sample 3-3). Therefore, the difference in color development characteristics between the two is the point of the maximum absorption wavelength. It was also confirmed from. Also, when the filled reagent bottle was visually observed, the purple sweet potato dye (Sample 3-2) exhibited a dark purple tone.

From the above results, it is shown that by producing a pigment composition using the red sweet potato-based mass roots according to the present invention as a raw material, an anthocyanin-based pigment exhibiting a bright red color tone completely different from that of a normal purple sweet potato pigment can be produced. It was. Its color development characteristics were very similar to those of the red radish dye anthocyanin dye.

[Example 4] "Evaluation of color development due to difference in pH"
The color development of the anthocyanin-based pigment composition derived from the red sweet potato strain prepared in the above example was evaluated when the pH was changed.

A test solution was prepared in the same manner as in Example 3 except that the pH value was changed to the value shown in FIG. 2, and the test solution was filled in a sample bottle for visual evaluation of color tone ( Figure 2).
As a result, it was confirmed that the red sweet potato dye (Sample 4-1) produced in the above-mentioned examples was capable of developing a bright bright red tone even when the pH was adjusted to any of 3 to 5. In particular, it was confirmed that the red radish dye (Sample 4-3) maintained vivid color development even at around pH 5, where the color development became lighter, with the red sweet potato dye (Sample 4-1).
On the other hand, the purple sweet potato dye (Sample 4-2), which is a comparative sample, has a darker purple color than the red sweet potato dye (Sample 4-1) and the red radish dye (Sample 4-3) regardless of the pH adjustment. It was confirmed that it exhibited a tinged color tone.

From the above results, it was confirmed that the red sweet potato lineage-based pigment composition according to the present invention is an anthocyanin pigment capable of producing a bright bright red color in a wide range of slightly acidic pH.

[Example 5] "Stability evaluation by absorbance ratio"
The stability of the anthocyanin compound was evaluated by calculating the absorbance ratio of 320 nm/530 nm for the anthocyanin pigment composition derived from the red sweet potato strain prepared in the above Examples.

For the red sweet potato dye prepared in Example 2, a test solution was prepared using a citrate buffer solution (pH 3.0) so that the E ^10% _{1 cm} value was 0.3 to 0.7, and the spectrophotometer ( Transmitted light colorimetry was performed using V-560, manufactured by JASCO Corporation, optical path length of measuring cell 1 cm). The _A320nm / _A530nm value was calculated from the measured absorption spectrum value.
As a comparative test, a test solution was prepared in the same manner as above using the purple sweet potato dye prepared in Example 2 or the red radish dye described in Example 3 (Table 11).
As a result, the red sweet potato dye (Sample 5-1) produced in the above-mentioned examples was compared with purple sweet potato dye (Sample 5-2) and red radish dye (Sample 5-3) at A _{320 nm} /A _{530 nm.} The value showed a remarkably high value.

From the above results and findings, it was shown that the dye composition using the red sweet potato line mass root as a raw material according to the present invention is an anthocyanin-based dye exhibiting excellent absorbance characteristics with excellent stability. The result was found to be consistent with the compositional characteristics of high content of the anthocyanin compound having a hydroxycinnamon acid modifying group shown in the above example.

[Example 6] "Evaluation of light resistance"
The anthocyanin-based pigment composition derived from the red sweet potato strain prepared in the above example was evaluated for the light stability of the pigment composition.

(1) "Preparation of beverage test solution"
Anthocyanin dyes with color value E ^10%, _{1 cm} value 80 equivalent 0.03% by mass, Brix 10 ° (fructose-glucose liquid sugar Brix 75 °: 13.3% by mass), and pH 3.0 (anhydrous citrate and trisodium citrate) The aqueous solution was prepared so as to be (adjusted with). As the anthocyanin dye, the red sweet potato dye prepared in Example 2, the purple sweet potato dye prepared in Example 2, or the commercially available red radish dye described in Example 3 was used.
The prepared aqueous solution was sterilized to reach 93° C., and a 200 mL PET bottle was hot-pack filled to obtain a PET bottle beverage as a test solution.

(2) "Light resistance test"
Each PET bottle test solution was irradiated with a white fluorescent lamp 20000 Lux at 25° C. for 3 days using a fluorescent lamp irradiator, and a stability test regarding light resistance was performed. Cultivation chamber CLH-301 (manufactured by Tommy Seiko Co., Ltd.) was used as the fluorescent lamp irradiator.
The test liquid before and after the stability test was subjected to transmitted light colorimetry at a measurement wavelength of 380 to 780 nm using a spectrophotometer (V-560, manufactured by JASCO Corporation, optical path length of the measurement cell was 1 cm). The dye residual ratio was calculated using the formula (4) (Table 12). Further, as a value for quantitatively evaluating the change in color tone before and after the stability test, the ΔE value was calculated using the above formula (2) and evaluated (Table 13). The results of visual observation of the PET bottle beverage are shown in FIG.

As a result, in the beverage containing the red sweet potato pigment (Sample 6-1) produced in the above example, even when exposed to a fluorescent lamp continuous irradiation environment of 20000 Lux for 3 days, the conventional sweet potato derived from Ayamurasaki Compared with the beverage containing the pigment (Sample 6-2) and the beverage containing the red radicon pigment (Sample 6-3), the residual dye ratio was significantly higher (Table 12). In addition, the ΔE value indicating the color tone change before and after the light resistance test is also lower than that of the conventional beverage containing sweet potato pigment derived from Ayamurasaki (Sample 6-2) (color tone change before and after the test). Was small) (Table 13). Visual observation of the test solution also confirmed the superiority of the effect of suppressing color change (Fig. 3).

Here, the light intensity of “20,000 lux” used in the stability test of this test is a strong light intensity corresponding to light abuse, which is 10 to 20 times the illuminance of fluorescent lamps used by ordinary retail stores. is there.
From the above results and findings, it was shown that the dye composition made from the red sweet potato line according to the present invention is an anthocyanin-based dye having extremely excellent stability against light irradiation. The result was found to be consistent with the compositional characteristics of high content of the anthocyanin compound having a hydroxycinnamon acid modifying group shown in the above example.

[Example 7] "Evaluation of heat resistance"
The anthocyanin-based pigment composition derived from the red sweet potato strain prepared in the above example was evaluated for the thermal stability of the pigment composition.

(1) "Preparation of beverage test solution"
A 200 mL PET bottle was hot-packed in the same manner as in Example 6 to prepare an aqueous solution containing an anthocyanin dye, and the obtained PET bottle beverage was used as a test solution.

(2) "Heat resistance test"
Each PET bottle test liquid was stored in a dark place under a high temperature condition of 50° C. for 7 days, and a stability test regarding heat resistance was performed.
With respect to the test liquid before and after the stability test, a transmitted light colorimetry at a measurement wavelength of 380 to 780 nm is performed using a spectrophotometer (V-560, manufactured by JASCO Corporation, optical path length of the measurement cell is 1 cm), and the measurement is performed. The residual dye ratio was calculated in the same manner as in Example 6. The results are shown in Table 14.
Further, the ΔE value indicating the change in color tone before and after the stability test was calculated and evaluated in the same manner as in the method described in Example 6. The results are shown in Table 15.

As a result, in the beverage containing the red sweet potato pigment (Sample 7-1) produced in the above example, the conventional sweet potato pigment derived from Ayamurasaki was used even when exposed to a high temperature environment of 50 ° C. for 7 days. The pigment residual rate was as high as or higher than that of the beverage containing (Sample 7-2). In addition, the ΔE value, which indicates the color change before and after the heat resistance test, should be about the same as or less than that of the conventional beverage containing sweet potato pigment derived from Ayamurasaki (Sample 7-2) (the color change before and after the test is small). ) Was confirmed.

Here, the temperature of “50° C.” used in the stability test according to the present test is about 8 times faster than the case of storing at 20° C. which is a general room temperature, and the temperature is about 8 times as fast as the chemical reaction. It is a corresponding temperature.
From the above results and knowledge, it was shown that the pigment composition of the present invention, which uses the red sweet potato strain as a raw material, is an anthocyanin-based pigment excellent in heat stability as in the conventional sweet potato pigments. When the results of the heat resistance test and the results of the light resistance test according to Example 6 are considered together, the compositional characteristics regarding the stability of the red sweet potato dye according to the present invention are light stability rather than thermal stability. It was recognized that it contributed greatly to.

[Example 8] "Sensory evaluation of odor"
The anthocyanin pigment composition derived from the red sweet potato strain prepared in the above Examples was evaluated for odor.

20 g each of the red sweet potato dye prepared in Example 2, the anthocyanin dye derived from purple sweet potato prepared in Example 2, and the commercially available red daikon dye described in Example 3 is filled in a sample bottle having a capacity of 30 g. Each sample (Sample 8-1 to Sample 8-3) was prepared. The sensory evaluation by blinds was performed on the aroma components emitted from the sample in the bottle. The results are shown in Table 16.

As a result, a sulfur odor peculiar to Japanese radish was perceived in the sample containing the red radish dye (Sample 8-3), and the sample containing the red sweet potato dye (Sample 8-1) produced in the above-mentioned example was used as a control. Similar to the sample containing the purple sweet potato pigment (Sample 8-2), the odor was hardly perceived and showed good odor characteristics.
From the above results, it was confirmed that the red sweet potato pigment according to the present invention is an anthocyanin-based pigment composition having good odor characteristics similar to those of a normal sweet potato pigment. In this respect, the red sweet potato dye according to the present invention was recognized as an anthocyanin-based dye composition having the same color tone as the red radish dye, which fundamentally overcomes the problem concerning the odor characteristics of the red radish dye.

[Example 9] "Evaluation of absorption characteristics when ingested as food and drink"
The anthocyanin pigment composition derived from the red sweet potato strains prepared in the above Examples was evaluated for absorption characteristics when ingested as a food or drink by an in vitro intestinal membrane permeability test.

(1) "Intestinal membrane permeability test"
With respect to the red sweet potato pigment prepared in Example 2, a test solution having a color value E ^10% _{1 cm} value of 5.0 and a pH of 6.8±0.2 was prepared using an HBSS buffer solution. This was placed on the lumen side of the Coca-2 cell sheet of POCA(R) small intestine absorption (CACO-2) (DS Pharma Biomedical Co., Ltd.), which is a cell assay kit for evaluating small intestinal absorption, and the base of the cell sheet was placed. The solution on the membrane side was sampled over time, and the absorbance was measured using a spectrophotometer (V-560, manufactured by JASCO Corporation, optical path length of 1 cm in the measurement cell). The absorbance measurement was performed at a wavelength of 500 nm in consideration of the maximum absorption wavelength of the red sweet potato dye.
As a comparative test, the purple sweet potato dye prepared in Example 2 was used instead of the red sweet potato dye, and 530 nm was adopted as the measurement wavelength in consideration of the maximum absorption wavelength of the purple sweet potato dye. The test was conducted in the same manner as in (Fig. 4).

As a result, it was shown that the red sweet potato pigment (Sample 9-1) produced in the above example was rapidly taken up by the intestinal membrane cells and permeated to the basement membrane side within a few hours. The permeation amount of the red sweet potato pigment into the intestinal membrane was significantly higher than that of the purple sweet potato pigment (Sample 9-2) derived from Ayamurasaki, which is a conventional technique. Specifically, the permeation amount of the red sweet potato pigment into the intestinal membrane reached about 2.1 times that of the purple sweet potato pigment after 3 hours and about 3.2 times that of the purple sweet potato pigment after 6 hours.

From the above results, it was revealed that the red sweet potato pigment according to the present invention is an anthocyanin pigment excellent in absorption characteristics when ingested as a food or drink. Its absorption efficiency was recognized to have significantly higher compositional properties than conventional purple sweet potato dyes.

[Example 10] "Preparation of sweet potato juice"
The sweet potato squeezed juice, which is an anthocyanin-based pigment composition, was prepared by using, as a raw material, the tuber roots of the novel sweet potato line which were excellent in bright red color development produced in the above examples.

(1) "Preparation of sweet potato juice"
The roots of Kyushu No. 187, which is the above-mentioned production line, were blanched, water was added and finely pulverized using a mixer. Next, saccharification treatment was performed, and solid-liquid separation was performed by centrifugation to collect a supernatant. After concentrating the recovered liquid under reduced pressure, a concentrated solution was prepared so as to have a Brix of 50°±5. This was subjected to heat sterilization at a temperature of 70° C. and subdivided to prepare a sweet potato juice.
As a comparative sample, a sweet potato juice was prepared in the same manner as described above except that the variety Ayamurasaki was used in place of Kyushu 187.

(2) "Evaluation of color development characteristics"
With respect to the above-mentioned squeezed juice, a solution diluted with Brix 10° using ion-exchanged water was prepared, and tristimulus values of the Hunter Lab color system were measured to evaluate lightness, saturation, and hue. The measurement and calculation of each value were performed in the same manner as in the method described in Example 3 (Table 17). The results of visual observation of the color tone are shown in FIG.

As a result, the prepared red sweet potato juice (Sample 10-1) has a and b values indicating hues on the red side and the yellow side, respectively, as compared with the normal purple sweet potato juice (Sample 10-2). The L value indicating the lightness and the CHROMA value indicating the saturation also showed high values. The HUE value of the red sweet potato juice (Sample 10-1) was 8.9 RP (color tone close to purple red in the JIS hue), and 2.1 RP (Sample 10-2) of the purple sweet potato juice (Sample 10-2). It showed a color tone that was significantly different from the reddish purple to reddish purple intermediate color tone in the JIS hue. Further, in visual observation, the red sweet potato juice (Sample 10-1) exhibited a bright and vivid red color, and the purple sweet potato juice (Sample 10-2) exhibited a dark purple color.

(3) "Sensory evaluation of taste"
The sweet potato juice prepared in (1) above was subjected to a blind taste sensory evaluation by 10 panelists. The results are shown in Table 18.
As a result, the red sweet potato juice (Sample 10-1) seems to have a slightly less sweetness and flavor than the Ayamurasaki juice (Sample 10-2), which is purple sweet potato, but the overall taste is good. It was confirmed that there was.

(4) "Summary"
From the above results, it was shown that by producing a squeezed liquid from the lump root of the red sweet potato line according to the present invention, a sweet potato squeezed liquid exhibiting a bright red color completely different from that of a normal purple sweet potato pigment can be produced. .. In addition, it was shown that the squeezed juice had a good flavor when eaten.

[Example 11] "Preparation of sweet potato paste"
A sweet potato paste, which is an anthocyanin-based pigment composition, was prepared using the mass roots of a novel sweet potato line having excellent bright red color development produced in the above example as a raw material.

(1) "Preparation of sweet potato paste"
The root of Kyushu No. 187, which is the above-mentioned production line, was sliced with a kitchen knife and steamed with a steamer. A sweet potato paste was manufactured by pressing this on a mesh with a rubber peller and back straining.
As a comparative sample, a sweet potato paste was prepared in the same manner as above except that the variety Ayamurasaki was used instead of Kyushu No. 187.

(2) "Evaluation of color development characteristics"
With respect to the prepared paste, the tristimulus values of the Hunter Lab color system were measured to evaluate the brightness, saturation, and hue. Each value was measured and calculated in the same manner as in the method described in Example 3 (Table 19). Further, the result of visually observing the color tone is shown in FIG.

As a result, the prepared red sweet potato paste (Sample 11-1) had the a value and the b value indicating the hue on the red side and the yellow side, respectively, as compared with the normal purple sweet potato paste (Sample 11-2). The L value indicating the brightness and the CHROMA value indicating the saturation also showed high values. The HUE value of red sweet potato paste (Sample 11-1) was 7.7 RP (intermediate color tone of reddish purple to purplish red in JIS hue), and 9.9P of purple sweet potato paste (Sample 11-2). It showed a color tone significantly different from (reddish purple color tone in JIS hue). Further, in visual observation, the red sweet potato paste (Sample 11-1) exhibited a bright and vivid red color, and the purple sweet potato paste (Sample 11-2) exhibited a dark purple color.

(3) "Sensory evaluation of taste"
The sweet potato paste prepared in (1) above was subjected to a sensory evaluation of taste by blinds by 10 panelists. The results are shown in Table 20.
As a result, the red sweet potato paste (Sample 11-1) has a slightly less sweet potato taste than the purple sweet potato Ayamurasaki paste (Sample 11-2), but has a good texture and texture as a whole. It was confirmed that it had a good taste.

(4) "Summary"
From the above results, it is possible to produce a sweet potato paste having a bright red color tone completely different from that of a normal purple sweet potato pigment by producing a paste-like viscous solid using the red sweet potato line mass root according to the present invention as a raw material. Shown. It was also shown that the paste had a good flavor when eaten.

[Example 12] "Preparation of sweet potato powder"
A sweet potato powder, which is an anthocyanin-based pigment composition, was prepared using the mass roots of a novel sweet potato line having excellent bright red color development produced in the above example as a raw material.

(1) "Preparation of sweet potato powder"
The sweet potato paste prepared in Example 11 was rolled, dried in an oven, and then pulverized in a mill to produce sweet potato powder.
As a comparative sample, a sweet potato powder was prepared in the same manner as described above except that the sweet potato paste of the variety Ayamurasaki was used in place of the sweet potato paste of Kyushu No. 187.

(2) "Evaluation of color development characteristics"
The hue of the prepared powder was evaluated by measuring tristimulus values in the Hunter Lab color system. The measurement and calculation of each value were carried out in the same manner as in the method described in Example 3. The results are shown in Table 21. The result of visually observing the color tone is shown in FIG.

As a result, the prepared red sweet potato powder (Sample 12-1) and purple sweet potato powder (Sample 12-2) showed the same color development characteristics as the sweet potato paste produced in Example 11. For details on the color tone, the HUE value of red sweet potato powder (Sample 12-1) is 7.7 RP (intermediate color tone of reddish purple to purplish red in JIS hue), and that of purple sweet potato powder (Sample 12-2). It showed a color tone significantly different from 9.9P (reddish purple color tone in JIS hue). Further, in visual observation, the red sweet potato powder (Sample 12-1) exhibited a bright and vivid red color, and the purple sweet potato powder (Sample 12-2) exhibited a dark purple color.

From the above results, it is shown that by producing a powdery powder from the red sweet potato line mass root according to the present invention, a sweet potato powder having a bright red color tone completely different from that of a normal purple sweet potato pigment can be produced. Was done.

[Example 13] "Analysis on the origin of pelargonidin-based anthocyanins"
In the process of producing the new red sweet potato line according to Example 1, analysis of the origin of the trait having a high content of pelargonidin anthocyanin was performed.

For the original varieties used for the line production of Kyushu No. 187 in Example 1 and each line generated in the production process (see FIG. 8), color tone observation in the mass root and pigment composition analysis of the anthocyanin pigment were performed. The analysis of the anthocyanin pigment was carried out by preparing an extract from the roots of the plant body of each line and subjecting it to HPLC / MS analysis in the same manner as in the method described in Example 1.

As a result, when we analyzed the individuals whose mass roots that appeared from the mating of the original varieties 9-series 89360-8 and 9-series 294 showed a bright red color, the individuals containing pelargonidin-based anthocyanins were about all the individuals of the generation. It was confirmed that they appeared independently at a rate of 12%. These results were in agreement with the color tone of tuberous roots.
In addition, it was confirmed that the 9-series 06353-19, which is the first-generation selected individual after the crossing of the original varieties, and the successor line, Kyushu 187, contain pelargonidin-based anthocyanins as the main anthocyanin pigment. It was. It was also confirmed that the tuberous root color tone of these strains was a bright red color tone.

From the above results, the original varieties 9-series 89360-8 and 9-series 294 have a pigment composition that does not contain pelargonidin-based anthocyanins. From the mating population obtained by mating these two strains, pelargonidin-based anthocyanins It was confirmed that individuals containing the above appeared with a frequency of about 12%. Since such lines containing pelargonidin anthocyanins do not appear in normal sweet potato line mating, it was speculated that there is a mechanism of action that causes the trait due to the genetic background generated by the combination of these original varieties. ..
In addition, it was confirmed that the trait containing a high amount of pelargonidin anthocyanin is a stable trait that is genetically transmitted even in the progeny.

[Example 14] "Analysis regarding origin of acylation modification mode"
In the process of producing the new red sweet potato line according to Example 1, analysis of the origin of the trait having a high content of hydroxycinnamic acid-modified anthocyanin was performed.

The anthocyanin pigment composition in the lump root was analyzed for the original cultivar used for the strain production of Kyushu No. 187 in Example 1 (see FIG. 8). The analysis of the anthocyanin pigment was carried out by preparing an extract from the roots of the plant body of each line and subjecting it to HPLC / MS analysis in the same manner as in the method described in Example 1. The results are shown in Table 23.

As a result, the ratio of hydroxycinnamic acid-modified anthocyanin of 9 series 89360-8 in the original variety showed a high value of 93.3% of all anthocyanin compounds. That is, it was shown that in this system, the acylation modification was a modification mode with a high hydroxycinnamic acid modification rate. In addition, the acylation modification mode of this system was such that R ₂ which is the acylation modification site of the general structural formula (I) of anthocyanin contained a large amount of anthocyanins acylated with caffeic acid.
On the other hand, the ratio of the hydroxycinnamic acid-modified anthocyanins of the original cultivar Kyushu 294 also showed a high value of 80.4% of all the anthocyanin compounds, but the mode of acylation modification is the production line after mating. It showed a different tendency from the Kyushu 187 system.

From the above results, it is inferred that the trait containing a high amount of hydroxycinnamon acid-modified anthocyanin of the red sweet potato strain produced in the present invention is a trait derived from one of the original varieties, 9 series 89360-8. It was.
It is the first finding that the analysis revealed that the original variety, Kyushu 89360-8, has a genetic background of the acylation modification mode regarding the anthocyanin composition.

[Example 15] "Creation of a progeny system from Kyushu 187"
It was verified whether the trait containing a high amount of pelargonidin anthocyanin of Kyushu No. 187 produced in Example 1 is a stable trait that is retained even in the subsequent strains.

Kyushu No. 187 produced in Example 1 was crossed with Kyushu 10302-25 strain, and individuals having a stronger bright red color development than their progeny were selected to produce Kyushu 14258-2. Further, in the same manner, Kyushu No. 187 was crossed with the 9 series 11184-10 to produce the 9 series 14259-13 (see FIG. 8).
Extracts were prepared from these tuberous roots and subjected to HPLC/MS analysis to analyze the compositional characteristics of the anthocyanin pigment. The analysis was performed in the same manner as in Example 1. The results are shown in Table 24. In addition, in the said table, the measurement result of Kyushu No. 187 in Example 1 is also shown together for comparison.

As a result, it was shown that 9 series 14258-2 and 9 series 14259-13 selected from the progeny population of Kyushu 187 are red sweet potato strains having a higher pelargonidin anthocyanin content ratio than Kyushu 187. In addition, it was shown that these progeny lines have a high rate of modification of hydroxycinnamic acid as an acylation modification, similar to Kyushu No. 187. In addition, in the acylation modification mode of these strains, R ₂ which is the acylation modification site of the general structural formula (I) of anthocyanins was a strain containing a large amount of anthocyanins acylated with caffeic acid.

From the above results, in the progeny population of the red sweet potato line produced by crossing the two original varieties used in Example 1, the pelargonidin-based anthocyanin content ratio was high by selecting individuals with a strong red color tone of the clump roots ( For example, it has been shown that it is possible to produce a line having a high hydroxycinnamic acid modification rate (for example, 85% or more, particularly 91% or more) (for example, 73% or more, particularly 87% or more).
From this, the novel trait containing a high amount of pelargonidin anthocyanin of the red sweet potato line produced by crossing the two original varieties used in Example 1 as a main pigment is genetically and stably transmitted even in the subsequent generations. It was shown to be a trait.

[Study Example 1] "Comparison with RSWP system"
Regarding the "LSWP" strain, which is a pelargonidin-based anthocyanin-containing strain disclosed in Patent Document 1, the information disclosed in the document was examined and compared with the production strain according to the present invention.

(1) “Pelargonidin-based anthocyanin content”
Kyushu No. 187, which is a line produced according to the present invention, was recognized as a line containing a high amount of pelargonidin-based anthocyanins because pelargonidin-based anthocyanins occupy about 85% of the total peak area of the anthocyanin compounds.
On the other hand, paragraph 0084 of Example 1 of Patent Document 1 describes that the content of pelargonidin-based anthocyanins in the RSWP strain is only 58 mL% with respect to the total anthocyanin compounds. In this respect, the difference in the type of aglycone anthocyanidin (pelargonidin, cyanidin, and peonidin) does not cause a large difference in the overall molecular weight of the anthocyanin compound, and in sweet potatoes, the difference in the anthocyanidin substantially changes the acylation modification mode. Considering that no significant difference occurs (see Example 1), the value of 58 mL% with respect to all anthocyanin compounds is less than 70% when converted to the content ratio (peak area ratio) with respect to all anthocyanin compounds. Was recognized.

(2) "Modification mode of acylation"
Kyushu No. 187, which is a produced strain according to the present invention, is a strain containing a high content of hydroxycinnamic acid-modified anthocyanins because the peak area of anthocyanin compounds modified with hydroxycinnamic acids occupies about 87% of the total anthocyanin compound peak area. Was accepted. In particular, for the compound of formula (I), have a compositional features anthocyanin compound of the modified style E ~ H where R ₂ site was acylated with caffeic acid is a hydroxy cinnamic acids account for about 80% of the total anthocyanins compound peak area Then it was admitted. Further, in Kyushu No. 187, it was recognized that the ratio of the peak area of the anthocyanin compound modified with another phenolic acid modifying group, hydroxybenzoic acid, was small, and it was only about 24% of the total peak area of the anthocyanin compound.

On the other hand, Examples 1 to 3 of Patent Document 1 and FIG. I was reviewing the HPLC chromatogram of absorbance 520nm according to 1, in RSWP system, anthocyanin compound of modifications manner B which R ₃ is acylated with a hydroxy benzoic acids of general structure of anthocyanins (I) is a major component There, the peak area of anthocyanin compounds modified manner E ~ H where R ₂ site was acylated with caffeic acid is a hydroxy cinnamic acids, were found to be less percentage of the total peak. The peak of the modified style C and D which R ₃ site was acylated with hydroxycinnamic acids, accepted even less than this.

(3) "Summary"
From the above comparison results, the RSWP strain, which is a pelargonidin-based anthocyanin-containing strain disclosed in Patent Document 1, has a greatly different acylation modification mode of the anthocyanin pigment composition from Kyushu No. 187, and is a hydroxybenzoic acid-modified anthocyanin. It was recognized that it is a sweet potato strain having a compositional characteristic of having a high content of anthocyanin modified with hydroxycatalyst acids, which contributes to the improvement of stability, as a major peak.
In addition, the RSWP strain of Patent Document 1 was recognized as a strain in which the content of pelargonidin anthocyanins was not sufficiently high as compared with the Kyushu 187 strain produced in the present invention. In this respect, it was confirmed that the results were in agreement with the fact that the color tone of the RSWP system was red with a purple hue.

1: Peak showing anthocyanin of modified style A 2: Peak showing anthocyanin of modified style B 3: Peak showing anthocyanin of modified style C 4: Peak showing anthocyanin of modified style D 5: Peak showing anthocyanin of modified style E 6: Peak showing modification mode F anthocyanins 7: Peak showing modification mode G anthocyanins 8: Peak showing modification mode H anthocyanins

FERM BP-22363
FERM BP-22364
FERM BP-22365

Claims (11)

A sweet potato plant containing a pelargonidin-based anthocyanin compound as a pigment component having the characteristics described below:
(1) A sweet potato plant belonging to the crossing group of Kyushu 89360-8 (FERM BP-22365) and Kyushu 294 (FERM BP-22364), or a progeny group whose parent is a sweet potato plant belonging to the crossing group It is a sweet potato plant,
(2) As the composition of the anthocyanin-based pigment contained in the root tuber, the peak area ratio of the pelargonidin-based anthocyanin compound in HPLC analysis is 70% or more with respect to the peak area of all the anthocyanin compounds, and
(3) As a composition of the anthocyanin dye contained in the root tuber, the peak area ratio of the anthocyanin compounds having a hydroxycinnamic acid modifying group in HPLC analysis is 50% or more with respect to the peak areas of all the anthocyanin compounds. The sweet potato plant according to claim 1, which has the characteristics described below:
As the composition of the anthocyanin dye contained in the root tuber, the peak area ratio of the anthocyanin compound having a caffeic acid modifying group as R ₂ in the structural formula (I) in HPLC analysis is 50% or more with respect to the peak area of all the anthocyanin compounds. Indicates:

(In the structural formula, R ₁ represents a hydrogen atom, a hydroxyl group, or a methoxy group, R ₂ represents a hydrogen atom or a caffeic acid modifying group, R ₃ represents a hydrogen atom, a p-hydroxybenzoic acid modifying group, A caffeic acid-modifying group or a ferulic acid-modifying group is shown. The sweet potato plant according to claim 1 or 2, which has the following characteristics:
Regarding an anthocyanin dye composition obtained by extracting tuberous root with 0.3% sulfuric acid water, when an aqueous solution containing the anthocyanin dye composition and having a pH of 3 is prepared with a citrate buffer solution, visible light It has a maximum absorption wavelength in the region from 505 to 520 nm, and
Regarding the anthocyanin dye composition obtained by extracting tuberous root with 0.3% sulfuric acid water, the anthocyanin dye composition was prepared so that the color value E ^10% _{1 cm} value was 0.3 to 0.7. A _{320 nm} / A _{530 nm} value is 3 or more when an aqueous solution having a pH of 3 is prepared with a citrate buffer solution. A method for producing a sweet potato plant containing a pelargonidin-based anthocyanin compound as a pigment component, which comprises the following steps:
(1) A step of crossing 9 lines 89360-8 (FERM BP-22365) and 9 lines 294 (FERM BP-22364) to obtain sweet potato plants belonging to the crossing group, or crossing the sweet potato plants belonging to the crossing group The process of obtaining sweet potato plants belonging to the progeny group,
(2) As a composition of the anthocyanin-based pigment contained in the root tuber, a step of selecting one having a pelargonidin-based anthocyanin compound peak area ratio in HPLC analysis of 70% or more with respect to the peak areas of all anthocyanin compounds, and
(3) As the composition of the anthocyanin-based pigment contained in the root tuber, select the one in which the peak area ratio of the anthocyanin compound having a hydroxycinnamic acid modifying group in HPLC analysis is 50% or more with respect to the peak area of all the anthocyanin compounds. Process. The plant body of the sweet potato plant according to any one of claims 1 to 3. The processed plant product, squeezed product, extract, or further processed product thereof according to claim 5. Sweet potato-derived anthocyanin-based pigment composition having the following characteristics:
As the composition of the anthocyanin-based dye, the peak area ratio of the pelargonidin-based anthocyanin compound in the HPLC analysis shows 70% or more with respect to the peak area of the total anthocyanin compound, and
As for the composition of the anthocyanin-based dye, the peak area ratio of the anthocyanin compound having a hydroxycaterous acid modifying group in the HPLC analysis shows 50% or more with respect to the peak area of all the anthocyanin compounds. The sweet potato-derived anthocyanin dye composition according to claim 7, which has the following characteristics:
As the composition of the anthocyanin dye, the peak area ratio of the anthocyanin compound having a caffeic acid modifying group as R ₂ in the structural formula (I) in HPLC analysis is 50% or more based on the peak area of all the anthocyanin compounds:

(In the structural formula, R ₁ represents a hydrogen atom, a hydroxyl group, or a methoxy group, R ₂ represents a hydrogen atom or a caffeic acid modifying group, R ₃ represents a hydrogen atom, a p-hydroxybenzoic acid modifying group, A caffeic acid-modifying group or a ferulic acid-modifying group is shown. Sweet potato-derived anthocyanin-based pigment composition having the following characteristics:
When an aqueous solution containing the anthocyanin-based dye composition and having a pH of 3 is prepared with a citrate buffer, it has a maximum absorption wavelength of 505 to 520 nm in the visible light region, and
When an aqueous solution containing the anthocyanin dye composition and having a pH of 3 with a citric acid buffer solution was prepared so that the color value E ^10% _{1 cm} value was 0.3 to 0.7, the A _{320 nm} / A _{530 nm} value was obtained. 3 or more. The anthocyanin-based pigment composition according to any one of claims 7 to 9, which has the following characteristics relating to absorbability in the body:
The amount is 1.5 times or more the cell permeation amount of the anthocyanin dye composition derived from Ayamurasaki. A method for producing a sweet potato-derived anthocyanin pigment composition, which comprises the step of using the plant as a raw material according to claim 5.

Images