WO2020172840A1 - Drug based on agriophyllum squarrosum alcohol extract - Google Patents

Abstract

A drug based on an Agriophyllum squarrosum alcohol extract, comprising the following components: 5-10 parts of geniposide, 10-20 parts of an Agriophyllum squarrosum alcohol extract, 3-5 parts of Radix Puerariae, 1-5 parts of Salvia miltiorrhiza, and 8-10 parts of Hovenia dulcis Thunb. The drug can effectively lower cholesterol levels.

Description

A medicine based on alcohol extract of sapin Technical field

The invention relates to the technical field of biomedicine, in particular to a medicine based on an alcohol extract of saponin.

Background technique

In the prior art, there is no traditional Chinese medicine that can effectively lower cholesterol. Therefore, there is a need to develop a traditional Chinese medicine that can effectively lower cholesterol without toxic side effects.

Summary of the invention

The purpose of the present invention is to provide a medicine based on an alcohol extract of saponin, which solves one or more of the above-mentioned existing technical problems.

A medicine based on the alcohol extract of sapin, comprising the following components in percentages by mass: 5-10 parts of geniposide, 10-20 parts of saponin alcohol extract, 3-5 parts of kudzu root, 1- 5 parts of Salvia and 8-10 parts of Hovenia dulcis.

In some embodiments, the following components are included in percentages by mass: 8 parts of geniposide, 15 parts of alcohol extract of sapin, 4 parts of Pueraria lobata, 2 parts of Salvia miltiorrhiza, and 9 parts of Hovenia dulcis.

In some embodiments, the preparation method of the alcohol extract of saponin includes the following steps:

A1. Crush the saponine through a 60-mesh sieve, mix the material-liquid ratio with the alcohol solution in a certain proportion, heat and reflux for 2 times, 2 hours each time, combine the filtrate, vacuum filter, concentrate the filtrate under reduced pressure, and vacuum dry to obtain saponol Extract.

In some embodiments, the alcohol solution is one or more of absolute ethanol, methanol, or glycerol.

In some embodiments, the alcohol solution is absolute ethanol.

In some embodiments, the ratio of material to liquid is 1:5-1:10.

In some embodiments, the extraction method of geniposide further includes the following steps:

B1. Weigh the pretreated gardenia powder, add ether solution and reflux for 1-2h, wherein the solid-liquid ratio of gardenia powder to ether solution is 1:5-1:30, filter with suction, and collect the filtrate;

B2. Add 5-10% diethyl ether to the filter residue and reflux, filter with suction, collect the filtrate, combine the filtrate, distill off diethyl ether, then add 20-30% ethanol, filter, distill under reduced pressure, and then dissolve in water , Suction filtration to obtain a crude extract of gardenia powder;

B3. Adsorb and separate the crude gardenia powder extract with a macroporous adsorption resin column, in which the volume ratio of mother liquor to macroporous adsorption resin is 1:10-1:20, and the mass fraction of ethanol is 5-20 for elution. The elution dose is 2-3 times the column volume to obtain the eluate, and the eluate is vacuum dried to obtain the gardenoside.

In some embodiments, the pretreatment method includes the following steps:

C1. Screening: Classify the size of gardenia seeds through a drum grading screen;

C2. Shelling: Put the classified gardenia seeds into the shelling machine for shelling;

C3. Drying: Use microwave drying equipment to dry the gardenia, so that the water in the gardenia seeds is vaporized and evaporated;

C4. Crushing: Use a crusher to crush the gardenia seeds, and pass through a 20-60 mesh sieve to obtain the gardenia powder.

Benefits:

Each component of the medicine of the embodiment of the application can be obtained by pure natural purification, and can effectively reduce total cholesterol.

detailed description

The following implementation cases illustrate the present invention in further detail.

Implementation case 1:

A medicine α based on the alcohol extract of sapin, comprising the following components in percentage by mass: 5 parts of geniposide, 10 parts of the alcohol extract of sapin, 3 parts of Pueraria lobata, 1 part of Salvia miltiorrhiza and 8 parts Hovenia dulcis.

Wherein, the preparation method of the alcohol extract of Sapin includes the following steps:

α1. Crush the sapphire through a 60-mesh sieve, mix the material-liquid ratio with anhydrous ethanol at a ratio of 1:5, heat and reflux twice for 2 hours each time, combine the filtrate, vacuum filter, concentrate the filtrate under reduced pressure, and dry under vacuum. Saponin alcohol extract.

The extraction method of geniposide includes the following steps:

α2, sieving: the size of gardenia seeds is classified through a drum grading screen;

α3. Shelling: Put the classified gardenia seeds into the shelling machine for shelling;

α4. Drying: Use microwave drying equipment to dry the gardenia, so that the water in the gardenia seeds is vaporized and evaporated;

α5. Crushing: use a crusher to crush the gardenia seeds and pass a 20-mesh sieve to obtain the gardenia powder.

α6. Weigh the pretreated gardenia powder, add the ether solution and reflux for 1 hour, where the solid-liquid ratio of the gardenia powder to the ether solution is 1:5, suction filter, and collect the filtrate;

α7. Add 5% ether to the filter residue and reflux, filter with suction, collect the filtrate, combine the filtrate, distill to remove the ether, then add 20% ethanol, filter, distill under reduced pressure, and then dissolve with water and filter with suction. To obtain a crude extract of gardenia powder;

α8. Adsorb and separate the crude gardenia powder extract with a macroporous adsorption resin column, where the volume ratio of mother liquor to macroporous adsorption resin is 1:10, and elution is carried out with an ethanol aqueous solution with a mass fraction of 5, and the elution dose is 2 Times the column volume to obtain the eluent, which is vacuum dried to obtain the gardenoside.

Implementation case 2:

A drug β based on the alcohol extract of sapin, comprising the following components in percentages by mass: 10 parts of geniposide, 20 parts of the alcohol extract of sapin, 5 parts of Pueraria lobata root, 5 parts of Salvia miltiorrhiza and 10 parts Hovenia dulcis.

Wherein, the preparation method of the alcohol extract of Sapin includes the following steps:

β1. Crush the sapphire through a 60-mesh sieve, mix with methanol at a ratio of 1:10 to the material-liquid ratio, heat and reflux twice for 2 hours each, combine the filtrate, vacuum filter, concentrate the filtrate under reduced pressure, and vacuum dry to obtain sapphire Alcohol extract.

The extraction method of geniposide includes the following steps:

β2, sieving: grading the size of gardenia seeds through a roller grading screen;

β3. Shelling: Put the classified gardenia seeds into the shelling machine for shelling;

β4. Drying: Use microwave drying equipment to dry the gardenia, so that the water in the gardenia seeds is vaporized and evaporated;

β5. Crushing: use a crusher to crush the gardenia seeds, and pass through a 60-mesh sieve to obtain the gardenia powder.

β6. Weigh the pretreated gardenia powder, add ether solution and reflux for 2h, in which the solid-liquid ratio of gardenia powder to ether solution is 1:30, suction filter, and collect the filtrate;

β7. Add 10% ether to the filter residue and reflux, filter with suction, collect the filtrate, combine the filtrate, distill to remove the ether, then add 30% ethanol, filter, distill under reduced pressure, and then dissolve in water and filter with suction. To obtain a crude extract of gardenia powder;

β8. Adsorb and separate the crude gardenia powder extract with a macroporous adsorption resin column, where the volume ratio of mother liquor to macroporous adsorption resin is 1:20, and eluted with an ethanol aqueous solution with a mass fraction of 20, and the elution dose is 3 Times the column volume to obtain the eluent, which is vacuum dried to obtain the gardenoside.

Implementation case 3:

A medicine γ based on the alcohol extract of sapin, comprising the following components in percentages by mass: 8 parts of geniposide, 15 parts of saponin alcohol extract, 4 parts of Pueraria lobata root, 2 parts of Danshen and 9 parts Hovenia dulcis.

Wherein, the preparation method of the alcohol extract of Sapin includes the following steps:

γ1. Crush the sarcophagus through a 60-mesh sieve, add the glycerin solution to the ratio of 1:8, heat and reflux for 2 times, 2 hours each time, combine the filtrate, vacuum filter, concentrate the filtrate under reduced pressure, and dry under vacuum An alcohol extract of saponin was obtained.

The extraction method of geniposide includes the following steps:

γ2, sieving: grading the size of gardenia seeds through a drum grading screen;

γ3. Shelling: Put the classified gardenia seeds into the shelling machine for shelling;

γ4. Drying: Use microwave drying equipment to dry the gardenia, so that the water in the gardenia seeds is vaporized and evaporated;

γ5. Crushing: Use a crusher to crush the gardenia seeds and pass a 40-mesh sieve to obtain the gardenia powder.

γ6. Weigh the pretreated gardenia powder, add ether solution and reflux for 1.5h, in which the solid-liquid ratio of gardenia powder to ether solution is 1:10, suction filter, and collect the filtrate;

γ7. Add 8% ether to the filter residue and reflux, filter with suction, collect the filtrate, combine the filtrate, distill the ether, then add 25% ethanol, filter, distill under reduced pressure, and then dissolve with water and filter with suction. To obtain a crude extract of gardenia powder;

γ8. Adsorb and separate the crude gardenia powder extract with a macroporous adsorption resin column, where the volume ratio of mother liquor to macroporous adsorption resin is 1:15, and elution is performed with an ethanol aqueous solution with a mass fraction of 10, and the elution dose is 2.5 Times the column volume to obtain the eluent, which is vacuum dried to obtain the gardenoside.

Test the drugs α, β, and γ in the implementation case:

Wistar rats were randomly divided into cages for 5 days to adapt to the environment and fed with basic feed. Randomly divided into 5 groups according to body weight: normal group, high fat group, α group, β group and γ group.

Animals in each group are kept in separate cages. The animal room is well ventilated and light. The light and dark cycle is 12h, the room temperature is 22℃, and the relative humidity is 40-50%. Clean drinking water and feeding are given once a day. The weight of the rats was measured and recorded every day, and the gavage was calculated according to the weight in the afternoon. The α group, the β group and the γ group were given the above-mentioned drugs for 15 days. The normal group was fed with basic feed, and the remaining 4 groups were fed with high-fat feed.

High fat feed formula: sodium cholate 0.5%, cholesterol 3%, lard 10%, basic feed 86.5%.

Cholesterol and sodium cholate are evenly mixed, the lard is evenly rubbed in the above-mentioned evenly mixed feed powder, and then water is fully kneaded and pressed into a fixed shape, dried, and set aside.

After fasting and drinking water for 16 hours on the 15th day, blood was taken from the femoral artery, and the blood was left to clot for 30 minutes and centrifuged for 15 minutes. The centrifugal speed is 3000r/min. Serum total cholesterol content is measured by COD-PAP colorimetric method, serum triglyceride content is measured by GPO-PAP colorimetric method, serum low-density lipoprotein cholesterol content is measured by polyvinyl sulfate precipitation method, serum high-density lipoprotein cholesterol material is phosphotungsten Determination by acid-magnesium precipitation method.

The test results are as follows:

It can be seen from the above table that the drugs α group, β group and γ group of the examples can effectively lower cholesterol.

The above expressions are only the preferred modes of the present invention. It should be pointed out that for those of ordinary skill in the art, without departing from the inventive concept of the present invention, several modifications and improvements can be made. These should also be regarded as the protection of the invention. Within range.

Claims (8)

A medicament based on alcohol extract of sapin, which is characterized in that it comprises the following components in percentage by mass: 5-10 parts of geniposide, 10-20 parts of saponin alcohol extract, 3-5 parts of Pueraria lobata, 1-5 parts of salvia and 8-10 parts of Hovenia dulcis. The medicament based on the alcohol extract of Sapin according to claim 1, characterized in that it comprises the following components in percentage by mass: 8 parts of geniposide, 15 parts of alcohol extract of Sabon, 4 parts Pueraria lobata root, 2 salvia miltiorrhiza and 9 Hovenia dulcis. A medicine based on an alcohol extract of saponin according to claim 1 or 2, wherein the preparation method of the alcohol extract of saponin comprises the following steps: A1. Crush the saponine through a 60-mesh sieve, mix the material-liquid ratio with the alcohol solution in a certain proportion, heat and reflux for 2 times, 2 hours each time, combine the filtrate, vacuum filter, concentrate the filtrate under reduced pressure, and vacuum dry to obtain saponol Extract. The medicine based on the alcohol extract of saponin according to claim 3, wherein the alcohol solution is one or more of absolute ethanol, methanol or glycerol. The medicine based on the alcohol extract of saponin according to claim 4, wherein the alcohol solution is absolute ethanol. The medicine based on the alcohol extract of saponin according to claim 3, characterized in that the ratio of material to liquid is 1:5-1:10. The medicine based on the alcohol extract of sapin according to claim 1 or 2, wherein the method for extracting geniposide further comprises the following steps: B1. Weigh the pretreated gardenia powder, add ether solution and reflux for 1-2h, wherein the solid-liquid ratio of gardenia powder to ether solution is 1:5-1:30, filter with suction, and collect the filtrate; B2. Add 5-10% diethyl ether to the filter residue and reflux, filter with suction, collect the filtrate, combine the filtrate, distill off diethyl ether, then add 20-30% ethanol, filter, distill under reduced pressure, and then dissolve in water , Suction filtration to obtain a crude extract of gardenia powder; B3. Adsorb and separate the crude gardenia powder extract with a macroporous adsorption resin column, in which the volume ratio of mother liquor to macroporous adsorption resin is 1:10-1:20, and the mass fraction of ethanol is 5-20 for elution. The elution dose is 2-3 times the column volume to obtain the eluate, and the eluate is vacuum dried to obtain the gardenoside. A medicine based on the alcohol extract of saponin according to claim 7, wherein the pretreatment method comprises the following steps: C1. Screening: Classify the size of gardenia seeds through a drum grading screen; C2. Shelling: Put the classified gardenia seeds into the shelling machine for shelling; C3. Drying: Use microwave drying equipment to dry the gardenia, so that the water in the gardenia seeds is vaporized and evaporated; C4. Crushing: Use a crusher to crush the gardenia seeds, and pass through a 20-60 mesh sieve to obtain the gardenia powder.