WO2020026261A1

WO2020026261A1 - System and method for encoding and decoding ethnic data into genetic codes

Info

Publication number: WO2020026261A1
Application number: PCT/IN2019/000025
Authority: WO
Inventors: Verma RAMA SHANKER; V Kamakoti; Gupta PIYUSH KUMAR; Biswas PRONAMA; Varadaraj SUDHA
Original assignee: Indian Institute of Technology Madras
Current assignee: Indian Institute of Technology Madras
Priority date: 2018-08-01
Filing date: 2019-07-29
Publication date: 2020-02-06
Anticipated expiration: 2021-02-01

Abstract

A system and method for encoding and decoding ethnic data including but not limited to, ancient mantras, slogans, and verses into genetic codes. A VKRSV encoding module for encoding the ethnic data into a genetic code wherein the genetic code stores the ethnic data securely for a longer time periods. The encoded data can be stored as a DNA nucleotide sequence in order to hide data in an original format. Further, the encoded data as a DNA nucleotide sequence is cloned into a pET-28a + plasmid vector of 5.369 kbps size. The cloned plasmid DNA is transformed into a bacterial system (E.coli) and stored at –80°C.

Description

SYSTEM AND METHOD FOR ENCODING AND DECODING ETHNIC

DATA INTO GENETIC CODES

TECHNICAL FIELD

[0001] The present invention generally relates to advanced data processing systems and computational approaches. The present invention also relates to DNA based data encoding and decoding techniques. The present invention further relates to systems and methods for securely encoding and decoding genetic data using computational approaches. The present invention more particularly related to a system and method for encoding and decoding ethnic data including but not limited to, ancient mantras, slogans, and verses into genetic codes.

BACKGROUND OF THE INVENTION

[0002] Every individual person in an ethnic group grows up in a culture hearing of tales on ancient lore involving many dimensions of experience in relationship to the environment and cosmos. Such ethnic data typically include for example, but not limited to, several ancient mantras, slogans and verses relating to an ethnic group or culture/heritage. Such ethnic data helps in shaping a person/individuaPs views on the culture, people and world. It also gives patterns for the situations the individual encounters throughout life. It is therefore storing and securing such ethnic data becomes critical in order to store the data in a secure environment and re-use the data on a longer timelines to ensure that the cultural data relating to the ethnic groups and transferred to their future generations safely.

[0003] With the advent of data processing systems and computer technology, most of the data are stored and produced as digital medium such as, PDFs, CD, DVDs and other digital storage forms. However, storing ethnic data on such general purpose digital storage mediums are not safe, secure and are prone to damage. It is therefore, storing and protecting such ethnic data on conventional digital storage mediums has become very challenging and is very essential to secure such ethnic data in a highly encrypted format.

[0004] The DNA based encoding and decoding techniques aids the conversion of any kind of narrative information or data into genetic code. In modem time, the hacking of secured data has become a serious problem in both IT and defense sectors. Therefore, hiding data in DNA nucleotides form can help for long-term storage without breaching in a compact volume. As, one gram of DNA carries 10⁸ TB (Terabytes) of data. Therefore, in few grams of DNA, one can store huge information (data) in a highly secure manner and compact manner. Methods and approaches for storing data in the form of a DNA nucleotide sequence is well-known in the art. Systems and methods are introduced to allow a person to experience systems of mythology within virtual reality environments generated by the computer system. However, such prior art systems are unable to provide an effective approach for storing ethnic data into genetic code and thereby enable encryption and decryption of genetic code for retrieval of ethnic data.

[0005] Based on the foregoing a need therefore exists for an improved system and method for securing ethnic data including ancient mantras, slogans and verses into genetic code using computational approaches. A need also exists for system and method for encoding and decoding ethnic data including but not limited to, ancient mantras, slogans, and verses into genetic codes, as discussed in greater detail herein. SUMMARY OF THE INVENTION

[0006] The following summary is provided to facilitate an understanding of some of the innovative features unique to the disclosed embodiment and is not intended to be a full description. A full appreciation of the various aspects of the embodiments disclosed herein can be gained by taking the entire specification, claims, drawings, and abstract as a whole.

[0007] Therefore, one aspect of the disclosed embodiment is to provide for an improved system and method for storing ethnic data into genetic code using computational approaches.

[0008] It is another aspect of the disclosed embodiment to provide for an improved DNA based encoding and decoding technique for securely storing and retrieving ethnic data in the form of genetic code.

[0009] It is further aspect of the disclosed embodiment to provide for an improved system and method for encoding and decoding ethnic data including but not limited to, ancient mantras, slogans, and verses into genetic codes.

[00010] The aforementioned aspects and other objectives and advantages can now be achieved as described herein. A system and method for encoding and decoding ethnic data including but not limited to, ancient mantras, slogans, and verses into genetic codes. A VKRSV encoding module for encoding the ethnic data into a genetic code wherein the genetic code stores the ethnic data securely for a longer time periods. The encoded data can be stored as a DNA nucleotide sequence having A, T, G, C nucleotide sequence format in order to hide data in an original format. Further, the encoded mantra as a DNA nucleotide sequence form (304 bps and 340 bps) is cloned into a pET-28a + plasmid vector of 5.369 kbps size. The cloned plasmid DNA is transformed into a bacterial system ( E.coh ) and stored at -80°C for long-term storage. A VKRSV decoding module for retrieving and decoding the encoded data in the form of DNA from bacteria by replacing nucleotide sequences (A, C, G, T) with digital patterns (00, 01, 10, and 1 1) respectively.

[00011] Note that the VKRSV encoding and decoding modules are computer applications (based on algorithms) running on an ethnic data processing system used for securely encoding and decoding the ethnic data into genetic codes. Encoding of ethnic data including, but not limited to, ancient mantras, slogans and verses in the for of genetic code is utmost important for storing and securing data up to a longer period of time. The encoded data can be stored as DNA having A, T, G, C nucleotide sequence format. The VKRSV encoding module is used for converting the ethnic data (text file) into A, T, G, C nucleotide sequence format by considering 2 bits at a time. During encoding, the decimal equivalent to two bits are kept as 0, 1, 2 and 3 where, these numerals indicate a particular nucleotide like 0 = A, 1 ~ C, 2 = G and 3 = T respectively. The glycerol stocks of bacterial culture containing encoded DNA can be stored in liquid nitrogen for a longer period of time up to thousand years. Later, the encoded nucleotide sequence can be retrieved from the isolated plasmid/vectors and the encoded information can be decoded using the same VKRSV decoding module.

BRIEF DESCRIPTION OF DRAWINGS

[00012] The drawings shown here are for illustration purpose and the actual system will not be limited by the size, shape, and arrangement of components or number of components represented in the drawings.

[00013] FIG. 1 illustrates a schematic representation of a system for encoding and decoding ethnic data including but not limited to, ancient mantras, slogans, and verses into genetic codes, in accordance with the disclosed embodiments; [00014] FIG. 2 illustrates a work flow diagram of a method for encoding and decoding ethnic data including but not limited to, ancient mantras, slogans, and verses into genetic codes, in accordance with the disclosed embodiments; and

[00015] FIG. 3 illustrates a graphical representation of the colony PCR and restriction mapping analysis carried out for selecting the desired recombinant transformants containing 304 bps and 340 bps nucleotide sequence among different recombinants, in accordance with the disclosed embodiments.

DETAILED DESCRIPTION

[00016] The particular values and configurations discussed in these nonlimiting examples can be varied and are cited merely to illustrate at least one embodiment and are not intended to limit the scope thereof.

[00017] The embodiments now will be described more fully hereinafter with reference to the accompanying drawings, in which illustrative embodiments of the invention are shown. The embodiments disclosed herein can be embodied in many different forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that tliis disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. Like numbers refer to like elements throughout. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

[00018] The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises" and/or "comprising," when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof.

[00019] Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

[00020] FIG. I illustrates a schematic representation of a system for encoding and decoding ethnic data including but not limited to, ancient mantras, slogans, and verses into genetic codes, in accordance with the disclosed embodiments. The system 100 can be effectively used for encoding and decoding ethnic data 110 including but not limited to, ancient mantras, slogans, and verses into genetic codes. A VKRSV encoding module for encoding the ethnic data 1 10 into a genetic code 120 wherein the genetic code 120 stores the ethnic data securely for a longer time periods. The encoded data 120 can be stored as a DNA nucleotide sequence having A, T, G, C nucleotide sequence format in order to hide data in an original format. Further, the encoded mantra as a DNA nucleotide sequence form (304 bps and 340 bps) is cloned into a pET-28a + plasmid vector 130 of 5.369 kbps size. The cloned plasmid DNA 130 is transformed into a bacterial system {E.coli) 140 and stored at -80C. A VKRSV decoding module for retrieving and decoding the encoded data from bacteria (in the DNA form) 140 by replacing nucleotide sequences (A, C, G, T) with digital patterns (00, 01, 10, and 1 1) respectively. [00021] FIG. 2 illustrates a work flow diagram 200 of a method for encoding and decoding ethnic data including but not limited to, ancient mantras, slogans, and verses into genetic codes, in accordance with the disclosed embodiments. Note that the VKRSV encoding and decoding modules are computer applications (based on algorithms) running on an ethnic data processing system 115 used for securely encoding and decoding the ethnic data 1 10 into genetic codes 120. Encoding of ethnic data 110 including, but not limited to, ancient mantras, slogans and verses in the form of genetic code 120 is utmost important for storing and securing data up to a longer period of time.

[00022] The encoded data 120 can be stored as DNA having A, T, G, C nucleotide sequence format. The VKRSV encoding module is used for converting the ethnic data (text file) 110 into A, T, G, C nucleotide sequence format by considering 2 bits at a time. During encoding, the decimal equivalent to two bits are kept as 0, 1 , 2 and 3 where, these numerals indicate a particular nucleotide like 0 = A, 1 = C, 2 = G and 3 = T respectively. The glycerol stocks of bacterial culture can be stored in liquid nitrogen for a longer period of time up to thousands of years. Later, the encoded nucleotide sequence can be retrieved from the plasmid/vectors and the encoded information can be decoded using the VKRSV decoding module.

[00023] FIG. 3 illustrates a graphical representation of the colony PCR and restriction mapping analysis carried out for selecting the desired recombinant transformants containing 304 bps and 340 bps nucleotide sequence among different recombinants, in accordance with the disclosed embodiments. Both 304 bps and 340 bps nucleotide sequences were synthesized and cloned into pET-28a +vector of 5.369 kbps size 130. The cloned product was confirmed by restriction mapping using Ba HI and Xbal restriction endonuclease enzymes. The cloned product was finally transformed into E.coli DH5a host stored in liquid nitrogen as glycerol stock for longer periods of time.

[00024] It will be appreciated that variations of the above-disclosed and other features and functions, or alternatives thereof, may be desirably combined into many other different systems or applications. Also that various presently unforeseen or unanticipated alternatives, modifications, variations or improvements therein may be subsequently made by those skilled in the art which are also intended to be encompassed by the following claims.

Claims

CLAIMS I/We Claim:

1. A system for encoding and decoding ethnic data into genetic codes, said system comprising:

An encoding module (VKRSV encoder) for encoding the ethnic data into a genetic code wherein the genetic code stores the ethnic data securely for a longer time periods; At least one DNA nucleotide sequence wherein the encoded data is stored as the DNA nucleotide sequence in order to hide data in an original format wherein the encoded data as a DNA nucleotide sequence form (304 bps and 340 bps) is cloned into a pET-28a + plasmid vector of 5.369 kbps size; and

2. The system of claim 1 wherein the cloned plasmid DNA is transformed into a bacterial system {E.coli) and stored at-80°C.

3. The system of claim 1 further comprising a decoding module (VKRSV decoder) for retrieving and decoding the encoded data from bacteria (the DNA form) by replacing nucleotide sequences with digital patterns respectively.

4. The system of claim 1 wherein the encoding and decoding modules are running on an ethnic data processing system used for securely encoding and decoding the ethnic data into genetic codes.

5. The system of claim 1 wherein the encoded data can be stored as DNA having A, T, G, C nucleotide sequence format.

6. The system of claim 1 wherein the encoding module is used for converting the ethnic data (text file) into A, T, G, C nucleotide sequence format by considering 2 bits at a time wherein the decimal equivalent to two bits are kept as 0, 1, 2 and 3.

7. The system of claim 1 wherein the glycerol stocks of bacterial culture ( E.coli ) can be stored in liquid nitrogen for a very long period of time.

8. The system of claim 1 wherein the encoded DNA nucleotide sequence can be retrieved from the plasmid/vectors and the encoded information can be decoded using the decoding module.

9. The system of claim 1 wherein the decoding module for retrieving and decoding the encoded data by replacing DNA nucleotide sequences (A, C, G, T) with digital patterns (00, 01, 10, and 1 1) respectively.