[go: up one dir, main page]

WO2020054204A1 - Antioxydant - Google Patents

Antioxydant Download PDF

Info

Publication number
WO2020054204A1
WO2020054204A1 PCT/JP2019/027489 JP2019027489W WO2020054204A1 WO 2020054204 A1 WO2020054204 A1 WO 2020054204A1 JP 2019027489 W JP2019027489 W JP 2019027489W WO 2020054204 A1 WO2020054204 A1 WO 2020054204A1
Authority
WO
WIPO (PCT)
Prior art keywords
extract
antioxidant
acid
oil
essential oil
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2019/027489
Other languages
English (en)
Japanese (ja)
Inventor
知宏 菅原
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyobo Co Ltd
Original Assignee
Toyobo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyobo Co Ltd filed Critical Toyobo Co Ltd
Priority to CN201980045137.XA priority Critical patent/CN112367969A/zh
Priority to JP2020546724A priority patent/JPWO2020054204A1/ja
Publication of WO2020054204A1 publication Critical patent/WO2020054204A1/fr
Anticipated expiration legal-status Critical
Priority to JP2023178167A priority patent/JP7694628B2/ja
Ceased legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/18—Antioxidants, e.g. antiradicals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations

Definitions

  • Reactive oxygen species refer to oxygen molecules and their related molecular species that have increased chemical reactivity compared to steady-state oxygen molecules, and are generated during energy metabolism in vivo.
  • the reactive oxygen species include superoxide (.O 2- ), hydroxy radical (.OH), hydroperoxy radical (HHO.), Nitric oxide (NO) as radical species, and hydrogen peroxide (H 2 O) as non-radical species. 2 ), singlet oxygen ( 1 O 2 ), peroxynitrite (ONOO ⁇ ), lipid hydroperoxide (LOOH) and the like.
  • reactive oxygen species are second messengers of the signal transduction system in the living body, and are molecular species indispensable for vital activities such as control of cell growth / differentiation and bactericidal action in macrophages and the like. On the other hand, excessive reactive oxygen species damage cells and tissues and are considered harmful.
  • GPX glutathione reductase
  • TRD thioredoxin reductase
  • PRX peroxiredoxin
  • antioxidant-related enzymes and redox-related factors are enhanced by ultraviolet irradiation stress.However, components that act on living organisms safely and effectively and promote the expression of antioxidant-related enzymes and redox-related factors are included. Expected.
  • Patent Document 1 it has been reported that kaempferol and quercetin belonging to flavonols widely distributed in tea leaf plants enhance the expression and activity of glutathione and thioredoxin systems in vivo.
  • Searching for components that act safely and effectively on antioxidant-related enzymes and / or redox-related factors in the living body is not limited to providing safer and more effective components to the market.
  • New ingredients that act on antioxidant-related enzymes and / or redox-related factors are important from the viewpoints of formulation diversification, expansion of formulation options, and synergistic effects of using different ingredients in combination. Needless to say, there is a need for development.
  • Japanese Alderwood is a legume deciduous tree that grows naturally in sunny wetlands such as forest margins and moorlands in the mountains south of the Tohoku region in Japan, and is also distributed overseas in China, Southeast Asia, etc. Cultivated.
  • the bark collected during the flowering season, washed with water, dried in the sun and cut to an appropriate length is called happi skin, and is taken in the private sector in anticipation of arthritis, back pain, diuresis, edema, tonic, For external use, it has been used as a cold compress for affected areas with decoction for tumors, bruises and arthralgias, or as bath water.
  • bark contains triterpene-based saponins (many julibrosides), flavonoids (geraldone, isookanin, luteolin, etc.), tannins and the like.
  • the present invention safely and efficiently reduces oxidative stress in a living body by promoting the expression of antioxidant-related enzymes and / or redox-related factors in a living body, maintains health, and
  • An object is to prevent various diseases and symptoms caused by oxidative stress.
  • the inventors of the present invention have conducted intensive studies and, as a result, have found that the extract of Alnus has an antioxidant effect, and have completed the present invention. It has been found that the extract of Alnus albicans enhances gene expression of SOD, TXN, PRX, TRD, GRD and glutaredoxin (GXN) among antioxidant-related enzymes and / or redox-related factors in vivo.
  • GXN glutaredoxin
  • the antioxidant of the present invention as an active ingredient for promoting the expression of an antioxidant-related enzyme and / or a redox-related factor in a living body, excessive stress in the living body can be obtained without giving stress to the applied living body. It is possible to eliminate various reactive oxygen species and reduce various oxidative stresses in a living body.
  • Item 1 An antioxidant containing a Neem tree extract.
  • Item 2. The antioxidant according to Item 1, wherein the extract of Albinia is an aqueous extract from a plant of the Alnus plant.
  • Item 3. Item 3.
  • the antioxidant of the present invention By using the antioxidant of the present invention, the antioxidant ability in a living body can be expected to be improved, and a skin external preparation for the purpose of improving the antioxidant ability can also be provided.
  • FIG. 4 is a diagram showing the results of an evaluation of the effect of enhancing expression of a target gene (SOD) in human normal neonatal epidermal keratinocytes in Example 1.
  • FIG. 4 is a diagram showing the results of an evaluation of the effect of enhancing expression of a target gene (TXN) in human normal neonatal epidermal keratinocytes in Example 1.
  • FIG. 3 is a diagram showing the results of an evaluation of the effect of enhancing expression of a target gene (GXN) in human normal neonatal epidermal keratinocytes in Example 1. It is a figure which shows the result of having evaluated the expression enhancement effect with respect to a target gene (SOD) in a normal human neonatal fibroblast in Example 2.
  • the Alnus extract means not only a plant of the Alnus genus plant, but also a cut product, a dry powder, and an extract thereof.
  • the “extract” includes both the state containing the extraction solvent and the state where the extraction solvent has been removed, and also includes the one that has been subjected to a purification treatment after the extraction solvent has been removed.
  • Neem tree extract is extracted from a plant of the genus Neem tree, typically the branches and leaves thereof, raw or dried and pulverized, and then used as an extraction solvent with water, preferably at a temperature of 70 to 100 ° C. It is obtained by performing extraction using water or an organic solvent. Extraction can be performed by combining water extraction and organic solvent extraction, if necessary. However, in consideration of the effect when applied to a living body, it is preferable to perform only water extraction.
  • a ground plant of the Alnus genus plant is extracted under heating and reflux.
  • the extraction conditions vary depending on the solvent used, for example, in the case of extraction with water, 1 to 100 parts by weight of water is used for 1 part by weight of Almond at a temperature of 4 to 100 ° C. for 10 minutes to 7 days. Extraction is preferably performed, and more preferably, using 5 to 20 parts by weight of water with respect to 1 part by weight of bark of Alnus oleracea at a temperature of 70 to 100 ° C. for 30 minutes to 2 hours. Further, from the viewpoint of further improving the extraction efficiency, pressurization, stirring, ultrasonic treatment and the like may be performed together with the extraction processing. Furthermore, after the extraction treatment, the residue may be separated from the residue by filtration or centrifugation.
  • the extraction solvent When the extraction solvent is distilled off under reduced pressure, it is usual to give a black-brown extract in the form of tar, and an excipient may be added as required.
  • the extraction solvent When the extraction solvent is non-toxic, for example, water, it can be used in the preparation while containing the extraction solvent, but the extract obtained by separating the extraction solvent can also be used in the preparation. The extract after separation of the extraction solvent can be used again for preparation by redissolving it in a suitable solvent, for example, water.
  • the extract thus obtained can be purified or improved in titer by a purification means, for example, fractional extraction, partitioning between two solvents, fractional adsorption / elution with an appropriate adsorbent or chromatography. .
  • the antioxidant of the present invention contains a Neem tree extract as an active ingredient.
  • the antioxidant effect in the present invention means that, when acted on cells and tissues in a living body, in addition to the scavenger effect of the antioxidant contained in the Alnus serrata extract, the antioxidant-related enzyme and / or redox-related factor in the living body is reduced. It also includes the effect of enhancing expression, particularly the effect of enhancing gene expression of antioxidant-related enzymes and redox-related factors. In addition, these actions exert an action of reducing the amount of active oxygen in cells.
  • the gene expression-enhancing action of antioxidant-related enzymes and redox-related factors is evaluated by adding the antioxidant of the present invention to cultured cells and quantifying the gene expression level in the cells using real-time PCR. be able to.
  • the amount of active oxygen in a cell is evaluated by incorporating a fluorescent substance responsive to active oxygen into cultured cells, adding the antioxidant of the present invention, and quantifying the intensity of fluorescence emitted from the cell. can do.
  • antioxidant-related enzymes and / or redox-related factors include superoxide dismutase (SOD), which eliminates reactive oxygen species, peroxidase, catalase, peroxiredoxin (PRX); and the oxidation and oxidation of glutathione (GSH) and thioredoxin (TXN).
  • SOD superoxide dismutase
  • GSH glutathione
  • TXN thioredoxin
  • TRD thioredoxin reductase
  • GXN glutaredoxin
  • glutathione peroxidase that reduces hydroperoxide generated by lipid oxidation, an intermediate having a carbonyl group (4) ⁇ , ⁇ -hydrogenase, etc.
  • oxidation Degrading proteases molecule that has undergone a wound, lipase, nuclease, chlorophyll degradation enzyme, pheophorbide a oxygenase and the like; methionine sulfoxide reductase that repair molecules oxidized by the active oxygen species include DNA repair enzymes and the like.
  • the extract of Neem tree can enhance the amount of antioxidation-related enzymes and / or redox-related factors in a living body, particularly by enhancing the gene expression levels of SOD, PRX, TXN, TRD, GRD and GXN. It is presumed that it reduces active oxygen generated in the body and exerts antioxidant ability.
  • the antioxidant of the present invention can contain other raw materials other than the extract of T. alder, within a range not to impair the effects of the present invention.
  • raw materials include water, excipients, antioxidants, preservatives, wetting agents, thickeners, buffers, adsorbents, solvents, emulsifiers, stabilizers, surfactants, lubricants, Water-soluble polymers, sweeteners, flavoring agents, acidulants, alcohols and the like can be mentioned.
  • the antioxidant of the present invention can contain other active ingredients as long as the effects of the present invention are not impaired.
  • alcohols include natural alcohols such as ethanol, isopropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol; Ethylene, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1,3-butylene glycol, Glycerin, bacilia Call, pentaerythritol, sorbitol, mannitol, glucose, polyhydric alcohols such as sucrose, and the like.
  • natural alcohols such as ethanol, isopropanol, lauryl alcohol, cet
  • Esters include isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, myristyl lactate, Examples include diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, and propylene glycol dioleate.
  • metal soap examples include aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate, and the like.
  • gum arabic As gums and water-soluble polymer compounds, gum arabic, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, dextrin, gelatin, pectin, starch, carrageenan, carboxyalkyl Chitin, chitosan, hydroxyalkyl chitin, low molecular weight chitosan, chitosan salt, sulfated chitin, phosphorylated chitin, alginic acid and its salts, hyaluronic acid and its salts, chondroitin sulfate, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, carboxy Ethyl cellulose sodium, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, poly
  • vitamin A1 As vitamins, retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A) in the vitamin A group, thiamine hydrochloride, thiamine sulfate (vitamin B1) in the vitamin B group, Riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acids, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C group, ascorbic acid and its derivatives, vitamin D group Ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotaxerol, vitamin E group, tocopherol and its derivatives, ubiquinones, vitamin K group, phytonadione (vitamin K1) Menaquinone (vitamin K2), menadione (vitamin K3),
  • Amino acids include valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine , Ornithine, histidine and the like, and their sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.
  • ascorbic acid or its derivative sulfur, placenta hydrolyzate, ellagic acid or its derivative, kojic acid or its derivative, glucosamine or its derivative, arbutin or its derivative, hydroxycinnamic acid or its derivative, glutathione, arnica Extracts, Ogon extract, Sohakuhi extract, Psychoextract, Boufu extract, Mannentake mycelium culture or extract thereof, Shinanoki extract, peach leaf extract, Agetsu extract, Kuddin extract, Jiyu extract, Touki extract, Yokunin extract, Oyster leaf extract, Daio extract , Peanut extract, hamamelis extract, maroni extract, hypericum extract, oil-soluble licorice extract and the like.
  • pentadecanoic acid glyceride coleus extract, gentian extract, matsukasa extract, royal jelly extract, kumazasa extract, t-flavanone, 6-benzylaminopurine, assembly extract, carpronium chloride, minoxidil, finasteride, adenosine, nicotinamide, Mulberry root extract, dirt extract, 5-aminolevulinic acid, and the like.
  • extracts or extracts of animals or plants and crude drugs include Asenyaku (Asenyaku), Ashitaba, Acerola, Altea, Arnica, Avocado, Amacha (Amcha), Aloe, Aloe vera, Nettle, Ginkgo (Ginkgo leaf, Ginkgo), Fennel ( Fencil), Turmeric (Utsukin), Usubasaishin (spicy), Plum (Umebai), Vladimir, Owaurushi, Noibara (real fruit), Hiokikoshi (extended life grass), Ougi (Yoki), Koganebana (Ougon), Yamazakura (cherry bark) ), Yellowfin (Huangkashio), Spinach (Yellow), Panax ginseng (Ginseng), Hypericum perforatum (Brother's cut grass), Odorikosou, Holland garashi, Orange, Itohimemegi (Enshi), Utsubogusa (Summer hay), Tsurukudami (Hanshu ,
  • Microbial culture metabolites include yeast extract, zinc-containing yeast extract, germanium-containing yeast extract, selenium-containing yeast extract, magnesium-containing yeast extract, rice fermentation extract, Euglena extract, lactic acid fermentation of skim milk powder, and the like.
  • inorganic pigments silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, mica titanium, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, Bengala, black iron oxide, gunjou, chromium oxide, chromium hydroxide, carbon black, calamine and the like.
  • Examples of the ultraviolet absorber include p-aminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, Examples include a pyrone derivative, a nucleic acid derivative, an allantoin derivative, a nicotinic acid derivative, a vitamin B6 derivative, oxybenzone, benzophenone, guaiazulene, shikonin, baicalin, baicalein, and berberine.
  • antioxidants As antioxidants, ascorbic acid and its salts, stearic acid esters, tocopherol and its ester derivatives, nordihydroguaselethenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), parahydroxyanisole, propyl gallate , Sesamol, sesamolin, gossypol and the like.
  • anti-inflammatory agents include ictamole, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d- or dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizinate and salts thereof. Glycyrrhetinic acid and salts thereof.
  • Examples of the disinfectant / disinfectant include acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof.
  • selenium disulfide, alkylisoquinolinium bromide, zinc pyrithione, biphenamine, thiantol, castal tincture show tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, sodium bromate, thioate Glycolic acid and the like.
  • flavors include natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, Iran essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guaiac wood essential oil, cumin Essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil, cedar essential oil, spearmint essential oil, western mint essential oil, large Fengxiang essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, Torubal sam essential oil, baturi essential oil, rose essential oil, palmarosa essential oil, cypress essential oil, hiba essential oil, sandalwood essential oil, petitgren essential oil, bay essential oil, vetiver essential oil
  • pigments and colorants examples include red cabbage pigment, red rice pigment, madder pigment, anato pigment, squid pigment, turmeric pigment, orange pigment, krill pigment, persimmon pigment, caramel, gold, silver, gardenia pigment, corn pigment, onion pigment , Tamarind pigment, spirulina pigment, whole buckwheat pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin and the like.
  • Examples of the nutritional enhancer include calcined shell calcium, cyanocorabamine, yeast, wheat germ, soybean germ, egg yolk powder, hemicellulose, heme iron and the like.
  • the dosage form of the present invention is arbitrary, and can be in the form of an ampule, capsule, powder, granule, pill, tablet, solid, liquid, gel, foam, emulsion, cream, ointment, sheet. And quasi-drugs such as mousse, skin and hair cosmetics, bath preparations, foods and drinks, and pharmaceuticals.
  • cosmetics and quasi-drugs include, for example, medicinal preparations for internal and external use, lotions, emulsions, creams, ointments, lotions, oils, basic cosmetics such as packs, facial cleansers and skin cleansers, Shampoos, rinses, hair treatments, hair creams, pomades, hair sprays, hair styling products, perm agents, hair tonics, hair cosmetics such as hair dyes, hair growth and hair restoration, foundations, white powder, whitening, lipsticks, blushers, eye shadows, Makeup cosmetics such as eyeliner, mascara, eyebrows and eyelashes, finishing cosmetics such as beautiful nails, perfumes, bath agents, other toothpastes, mouth fresheners / gargles, liquid odor and deodorants, Sanitary goods, sanitary cotton, wet tissue, and the like.
  • basic cosmetics such as packs, facial cleansers and skin cleansers, Shampoos, rinses, hair treatments, hair creams, pomades, hair sprays, hair styling products, perm agents, hair tonics, hair
  • Foods and drinks include, for example, soft drinks, carbonated drinks, nutritional drinks, fruit drinks, drinks such as lactic acid drinks, ice cream, ice sorbet, shaved ice, etc., buckwheat, udon, harusame, gyoza skin, sweet potato skin, Chinese Noodles such as noodles, instant noodles, candy, candy, gum, chocolate, tablets, snacks, biscuits, jellies, jams, creams, baked goods, baked goods, crabs, salmon, clams, tuna, sardines, shrimp, Fish products such as bonito, mackerel, whale, oyster, saury, squid, red mussel, scallop, abalone, sea urchin, salmon roe, tocobushi, fish and livestock processed foods such as kamaboko, ham, sausage, milk powder, processed milk, fermented milk, etc.
  • Chinese Noodles such as noodles, instant noodles, candy, candy, gum, chocolate, tablets, snacks, biscuits, jellies, jams
  • Products salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing, etc.
  • Processed fats, sauces, seasonings such as sauces, curry, stew, oyakodon, porridge, porridge, porridge, Chinese rice bowl, katsudon, tendon, unadon, hayashi rice, oden, marbodough, gyudon, meat sauce, egg soup, omelet, Examples include retort pouch foods such as gyoza, shomai, hamburger and meatballs, various forms of health and nutritional supplements, health foods, tablets, capsules, drinks, troches and the like.
  • the antioxidant of the present invention is suitably applied to humans, but can be applied to animals other than humans as long as the respective effects can be expected.
  • the aspect of the antioxidant of the present invention is not particularly limited, for example, face wash, cleanser, lotion (eg, whitening lotion), cream (eg, vanishing cream, cold cream), milky lotion, gel, beauty serum , Pack (for example, jelly-like peel-off type, paste-type wipe-off type, powdery rinse-out type), face mask, cleansing, foundation, lipstick, lip balm, lip gloss, lip liner, blush, makeup base, shaving lotion, sunscreen, After sun lotion, deodorant lotion, body lotion (including hand care lotion and foot care lotion), body oil and the like.
  • lotion eg, whitening lotion
  • cream eg, vanishing cream, cold cream
  • milky lotion gel
  • beauty serum Pack
  • face mask for example, jelly-like peel-off type, paste-type wipe-off type, powdery rinse-out type
  • face mask for example, jelly-like peel-off type, paste-type wipe-off type, powdery rinse-out type
  • cleansing foundation, lipstick, lip balm, lip gloss, lip liner, blush, makeup
  • the aqueous extract of bark tree bark used in the following examples was prepared by the method disclosed in JP-A-2018-58783.
  • Example 1 Evaluation of the effect of enhancing the expression of a target gene in human normal neonatal epidermal keratinocytes The effect of increasing the expression of a target gene was evaluated by the following test method using the extract of Neem tree as a test sample.
  • the target gene in this example means SOD, PRX, TXN, TRD, GRD and GXN.
  • a normal human neonatal keratinocyte (NHEK) is seeded on a T75 flask coated with collagen by Collagen Coating Solution (manufactured by Toyobo Co., Ltd.), and a medium for growing normal human keratinocyte (HuMedia-KG2) is added. And cultured at 37 ° C., 10% CO 2 . The medium was replaced as appropriate, and the culture was continued until the confluence reached 80% or more. After removing the medium and rinsing with PBS, the cells were collected by trypsinization. Cells were seeded in a 96-well plate at 1 ⁇ 10 4 cells / well and cultured at 37 ° C., 10% CO 2 for 1 day.
  • the medium was removed, the test sample was replaced with a medium containing a predetermined concentration, and incubated at 37 ° C., 10% CO 2 for 72 hours. After the culture, the cells were collected, and cDNA was prepared from the cells using SuperPrep (registered trademark) Cell Lysis & RT Kit for qPCR (manufactured by Toyobo Co., Ltd.).
  • the primers used were the nucleotide sequences shown in SEQ ID NOs: 1 to 6.
  • the instrument used was 7500 ⁇ Fast ⁇ Real-Time ⁇ PCR ⁇ System (Applied Biosystems).
  • the expression level of the target gene was normalized using the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene as an internal standard (SEQ ID NOs: 13 to 14).
  • GPDH glyceraldehyde-3-phosphate dehydrogenase
  • Example 2 Evaluation of the effect of enhancing expression of the target gene in human normal neonatal fibroblasts
  • T-flasks were inoculated with human normal neonatal fibroblasts (NHDF), and D-MEM (+ 10% FBS) was added.
  • NHDF human normal neonatal fibroblasts
  • D-MEM (+ 10% FBS
  • the medium was replaced as appropriate, and the culture was continued until the confluence reached 80% or more.
  • the cells were collected by trypsinization. Cells were seeded in a 96-well plate at 1 ⁇ 10 4 cells / well and cultured at 37 ° C., 5% CO 2 for 1 day.
  • the medium was removed, the test sample was replaced with a medium containing a predetermined concentration, and incubated at 37 ° C., 5% CO 2 for 72 hours. After the culture, the cells were collected, and cDNA was prepared from the cells using SuperPrep (registered trademark) Cell Lysis & RT Kit for qPCR (manufactured by Toyobo Co., Ltd.).
  • the nucleotide sequences shown in SEQ ID NOs: 1 to 12 were used.
  • the instrument used was 7500 ⁇ Fast ⁇ Real-Time ⁇ PCR ⁇ System (Applied Biosystems).
  • the expression level of the target gene was normalized using the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene as an internal standard (SEQ ID NOs: 13 to 14).
  • GPDH glyceraldehyde-3-phosphate dehydrogenase
  • Example 3 Evaluation of Reducing Activity of Reactive Oxygen in Human Normal Neonatal Fibroblasts T75 flasks were inoculated with human normal neonatal fibroblasts (NHDF), and D-MEM (+ 10% FBS) was added thereto. ° C., and cultured in 5% CO 2. The medium was replaced as appropriate, and the culture was continued until the confluence reached 80% or more. After removing the medium and rinsing with PBS, the cells were collected by trypsinization. The cells were seeded in a 96-well plate at 3 ⁇ 10 4 cells / well and cultured at 37 ° C., 5% CO 2 for 1 day.
  • H 2 DCFDA 2 ′, 7′-dichlorodifluorofluorescein diacetate
  • test sample and HBSS containing hydrogen peroxide at a predetermined concentration were replaced, and the mixture was further incubated at 37 ° C. and 5% CO 2 (in a dark place).
  • the test sample was applied to the face of the subject, and cells were obtained from the beard in the application area and evaluated. Specifically, before application of the test sample and 3 hours after application, the beard in the application area was removed from the subject, and hair follicle cells were collected. The cells were stored by immersion in RNAlater (manufactured by Qiagen) and stored at 4 ° C. until RNA extraction. After completion of collection of the hair follicle cells, total RNA was extracted from the hair follicle cells using RNeasy plus micro kit (manufactured by Qiagen) according to the attached protocol.
  • cDNA was prepared according to the attached protocol using ReverseTra @ Ace (registered trademark) qPCR @ RT @ Master @ Mix (manufactured by Toyobo Co., Ltd.). Based on the obtained cDNA, real-time PCR analysis was performed using THUNDERBIRD ⁇ SYBR (registered trademark) ⁇ qPCR ⁇ Mix ⁇ (manufactured by Toyobo Co., Ltd.). The composition of the reaction solution was in accordance with the package insert. However, the reaction solution volume was 20 ⁇ l / well, and the cDNA volume was 2 ⁇ l.
  • the reaction cycle conditions were: 95 ° C., 1 minute ⁇ (95 ° C., 15 seconds ⁇ 60 ° C., 45 seconds) ⁇ 40 ⁇ 95 ° C., 15 seconds ⁇ 60 ° C., 1 minute ⁇ 95 ° C., 15 seconds.
  • the primers used were the nucleotide sequences shown in SEQ ID NOs: 7 and 8.
  • the instrument used was 7500 ⁇ Fast ⁇ Real-Time ⁇ PCR ⁇ System (Applied Biosystems).
  • the expression level of the target gene was normalized using the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene as an internal standard (SEQ ID NOs: 13 to 14). The relative expression was calculated by dividing the standardized expression level of each target gene by the expression level of the gene in the negative control before application of the test sample.
  • GPDH glyceraldehyde-3-phosphate dehydrogenase
  • Example 5 Formulation of external preparation for skin
  • the following shows a formulation example of the external preparation for skin in the present invention. All of these preparations are expected to have an effect of enhancing the expression of antioxidant-related enzyme genes caused by the Alnus serrata extract, and are effective as antioxidants.
  • lotion was produced by a conventional method.
  • ⁇ Purified water 89.80 g ⁇ Glycerin: 3.00 g ⁇ Phenoxyethanol ⁇ ⁇ ⁇ 0.20g -Butylene glycol: 5.00 g ⁇ Pentylene glycol ⁇ ⁇ ⁇ 1.00g ⁇ Neem tree extract ⁇ ⁇ ⁇ 1.00g
  • Gel A gel was produced according to the following composition by a conventional method.
  • ⁇ Purified water 88.50 g ⁇ Carbomer ⁇ ⁇ ⁇ 0.30g ⁇ Xanthan gum ⁇ ⁇ ⁇ 0.10g ⁇ Arginine ⁇ ⁇ ⁇ 0.40g ⁇
  • Glycerin 5.00 g ⁇ Phenoxyethanol ⁇ ⁇ ⁇ 0.20g -Butylene glycol: 5.00 g ⁇ Neem tree extract ⁇ ⁇ ⁇ 0.50g
  • Cream According to the following composition, a cream was produced by a conventional method.
  • ⁇ Purified water 58.50 g ⁇ Butylene glycol ⁇ ⁇ ⁇ 10.00g ⁇ Glycerin: 5.00 g ⁇ Phenoxyethanol ⁇ ⁇ ⁇ 0.20g ⁇ Ethylhexyl glycerin: 0.20 g ⁇ Squalane ⁇ ⁇ ⁇ 10.00g ⁇ Olive oil ⁇ ⁇ ⁇ 10.00g ⁇ Behenyl alcohol: 2.50 g ⁇ Polyglyceryl pentastearate-10: 1.90 g ⁇ Na stearoyl lactate ⁇ ⁇ ⁇ 0.60g ⁇ Cetyl palmitate ⁇ ⁇ ⁇ 1.00g ⁇ Neem extract: 0.10 g
  • the oxidative stress of the living body can be reduced safely and efficiently, health can be maintained, and oxidative stress can be maintained. It is possible to prevent various diseases and symptoms caused by, and it is particularly useful as a cosmetic or a quasi-drug in application to a skin external preparation.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dermatology (AREA)
  • Epidemiology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • Birds (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Medical Informatics (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

L'invention concerne un antioxydant pour réduire de manière sûre et efficace le stress oxydatif in vivo par la favorisation de l'expression d'une enzyme antioxydante ou d'un facteur lié à l'oxydo-réduction in vivo pour ainsi maintenir une bonne santé et prévenir diverses maladies et symptômes provoqués par le stress oxydatif. L'antioxydant contient un extrait d'Albizia julibrissin et présente, en particulier, un effet favorisant l'expression d'un gène d'enzyme antioxydante et/ou d'un gène d'un facteur lié à l'oxydo-réduction.
PCT/JP2019/027489 2018-09-14 2019-07-11 Antioxydant Ceased WO2020054204A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN201980045137.XA CN112367969A (zh) 2018-09-14 2019-07-11 抗氧化剂
JP2020546724A JPWO2020054204A1 (ja) 2018-09-14 2019-07-11 抗酸化剤
JP2023178167A JP7694628B2 (ja) 2018-09-14 2023-10-16 抗酸化剤

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2018-172325 2018-09-14
JP2018172325 2018-09-14

Publications (1)

Publication Number Publication Date
WO2020054204A1 true WO2020054204A1 (fr) 2020-03-19

Family

ID=69778583

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2019/027489 Ceased WO2020054204A1 (fr) 2018-09-14 2019-07-11 Antioxydant

Country Status (3)

Country Link
JP (2) JPWO2020054204A1 (fr)
CN (1) CN112367969A (fr)
WO (1) WO2020054204A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114470050B (zh) * 2022-03-21 2023-09-05 上海相宜本草化妆品股份有限公司 一种抗敏、舒缓、止痒和/或修复皮肤的中药复方组合物
CN115501156B (zh) * 2022-09-28 2023-12-29 福建片仔癀化妆品有限公司 一种具有美白舒缓功效的化妆品原料组合物及其评价体系
CN115944571B (zh) * 2023-01-10 2024-04-02 河北纳科生物科技有限公司 一种促头发生长和修护发根的精华喷雾及其制备方法
CN119236047B (zh) * 2024-09-29 2025-08-12 江苏春江生物科技有限公司 一种提高免疫力的中草药组合物及其制备方法和应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000128798A (ja) * 1998-10-23 2000-05-09 Seiwa Yakuhin Kk 活性酸素消去作用剤およびアルドースリダクターゼ阻害作用剤
JP2009242296A (ja) * 2008-03-31 2009-10-22 Naris Cosmetics Co Ltd メラニン産性抑制剤・コラーゲン産生促進剤
JP2010235548A (ja) * 2009-03-31 2010-10-21 Maruzen Pharmaceut Co Ltd 抗酸化剤、抗炎症剤、美白剤、抗老化剤、育毛剤、及び抗肥満剤、並びに化粧料、及び飲食品
US20140377390A1 (en) * 2013-06-24 2014-12-25 Caster Cosmetic Compositions Comprising Plant Extracts for Combating Skin Aging
JP2015509072A (ja) * 2011-09-27 2015-03-26 セダーマ ネムノキ抽出物の新規な美容的使用およびそれに応じた局所組成物

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008018142A1 (fr) * 2006-08-10 2008-02-14 Wood One Co., Ltd. Composition antioxydante contenant un composant issu de l'écorce d'un arbre appartenant au genre acacia
KR101591872B1 (ko) * 2014-01-20 2016-02-04 주식회사 신라비앤에이치 항염, 항알러지 및 항산화 효능이 우수한 Ge-8 혼합물을 함유하는 화장료 조성물
CN105342916B (zh) * 2015-11-19 2019-04-16 伽蓝（集团）股份有限公司 一种植物提取物组合物及其制备方法和应用

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000128798A (ja) * 1998-10-23 2000-05-09 Seiwa Yakuhin Kk 活性酸素消去作用剤およびアルドースリダクターゼ阻害作用剤
JP2009242296A (ja) * 2008-03-31 2009-10-22 Naris Cosmetics Co Ltd メラニン産性抑制剤・コラーゲン産生促進剤
JP2010235548A (ja) * 2009-03-31 2010-10-21 Maruzen Pharmaceut Co Ltd 抗酸化剤、抗炎症剤、美白剤、抗老化剤、育毛剤、及び抗肥満剤、並びに化粧料、及び飲食品
JP2015509072A (ja) * 2011-09-27 2015-03-26 セダーマ ネムノキ抽出物の新規な美容的使用およびそれに応じた局所組成物
US20140377390A1 (en) * 2013-06-24 2014-12-25 Caster Cosmetic Compositions Comprising Plant Extracts for Combating Skin Aging

Also Published As

Publication number Publication date
JP2023171681A (ja) 2023-12-01
JPWO2020054204A1 (ja) 2021-08-30
CN112367969A (zh) 2021-02-12
JP7694628B2 (ja) 2025-06-18

Similar Documents

Publication Publication Date Title
JP4205188B2 (ja) メイラード反応阻害剤
JP3966922B2 (ja) 線維芽細胞増殖促進剤
JP5018171B2 (ja) 植物由来の賦活化剤及び細胞外マトリックス産生促進剤
JP3966924B2 (ja) 植物抽出物含有線維芽細胞増殖促進剤
JP7694628B2 (ja) 抗酸化剤
JP7658391B2 (ja) アンチポリューション剤及び皮膚外用組成物
JP7643335B2 (ja) ネムノキ抽出物含有組成物
JP4100911B2 (ja) グルタミン酸デカルボキシラーゼ活性化剤
JP2002241293A (ja) メイラード反応阻害剤
JP2004189663A (ja) メイラード反応阻害剤
JP2003095913A (ja) 化粧料組成物又は飲食品
JP5901597B2 (ja) 含硫ニンニク成分を高含有する加工ニンニク抽出物及びその製造方法、これを含む医薬品、食品、飲料、化粧料
JP2000128728A (ja) 化粧料組成物
JP2004107286A (ja) 化粧料組成物及び美容・健康食品組成物
JP2013139392A (ja) 皮膚老化抑制剤
JP4931356B2 (ja) グルタミン酸デカルボキシラーゼ活性化剤
JP3967103B2 (ja) リパーゼ活性抑制剤
JP2004026743A (ja) ポリリジン製剤およびこれを含有する化粧料組成物
JP6831549B2 (ja) Ａｂｃａ１２遺伝子発現促進剤
JP2007022958A (ja) イカ又はナマズ由来アテロコラーゲン及び／又はその誘導体及びその製造方法、並びに該アテロコラーゲン及び／又はその誘導体を配合する化粧料
JP2013166785A (ja) 植物由来ストレス軽減剤
JP2006316069A (ja) ニキビ用化粧料組成物
JP4169814B2 (ja) リュウゼツラン又はサイザル抽出物含有皮膚外用剤
JP7600521B2 (ja) 抗炎症剤
JP5578160B2 (ja) 植物由来の賦活化剤及び細胞外マトリックス産生促進剤

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 19860465

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 2020546724

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 19860465

Country of ref document: EP

Kind code of ref document: A1