WO2020046009A1 - Method for storing digital information in dna molecule, and apparatus for same - Google Patents

Abstract

Disclosed are a method for storing digital information in a DNA molecule, and an apparatus for same. A method for storing information on the basis of a DNA molecule, according to one embodiment of the present invention, comprises the steps of: receiving digital information; and storing the received digital information by using pre-synthesized DNA fragments of a predetermined length corresponding to first and second values of digital data.

Description

Method and apparatus for storing digital information in DNA molecules

The present invention relates to a DNA molecule-based information storage technology, and more particularly, to a DNA molecule-based that can store digital information in a DNA molecule by using a DNA fragment including a sequence pattern preset according to a digital data value. An information storage method and apparatus therefor.

The global data volume is expected to reach 40 ZB by 2020. Since 2007, the amount of data produced worldwide has exceeded that of storage, requiring a new type of storage. Magnetic tape, which is widely used as a long-term recording medium, has a limited data storage life of about 10 years, which requires continuous maintenance and management costs. In the case of semiconductor storage devices, HDDs and SSDs are typical, and HDDs are extremely vulnerable to shocks and have a lifespan of 250,000 hours and a maximum capacity limit. It is known to be short.

As an alternative technology that can solve the limitation of data storage density faced by existing information storage media, DNA sequencing has been mentioned as a long-term preservable storage medium for super-capacity information, and found the possibility of a new technology for storing super-capacity data in DNA structure. . DNA, as is well known, is contained within cells, the smallest unit of an organism, and contains all the genetic information. According to the information that DNA has, all living things move as programmed. In humans, DNA contained in one single cell is composed of 3 billion pairs of nucleotide sequences, which is about 1TB in size when the size of the genetic information decoded. One single cell contains two strands of DNA, 2nm wide and 3m long. Therefore, DNA, the next generation biostorage that can theoretically store more than EB (10 ¹⁸ ), is very suitable as a biomaterial for storing information intensively. The shelf life is more than 1,000 years and it is expected to be easy to store at low cost.

Recently, the explosive amount of data exceeds the capacity of the storage medium, causing overload, and thus, attempts to develop new storage media continue. Among them, the attempt to develop a new storage medium using DNA is very interesting. When DNA is used as a storage medium, the data storage density, which is a disadvantage of the existing storage medium, can be exceeded, and information can be stably stored even in a physical shock.

One embodiment technique for storing information in conventional DNA uses a method of storing information in the A, T, G, and C base portions of a DNA molecule, and the prior art uses a specific sequence for expressing information in order to store desired information. It is necessary to synthesize the information accurately, and because the accuracy of the synthesis cannot be guaranteed, the same information is synthesized in a large number of DNA molecules, and the long information is divided into small pieces and overlapped with each other to record the information.

This conventional technique is not a method of storing accurate information using a single molecule because it uses a large number of molecules to store information, and ultimately, the information must be restored as if a large number of molecules are voting. . The fundamental reason for this approach is that there is a limit to the accuracy of oligonucleotide synthesis.

Embodiments of the present invention provide a DNA molecule-based information storage method and apparatus for storing digital information in a DNA molecule by using a DNA fragment including a sequence pattern preset in correspondence with a digital data value.

DNA molecule-based information storage method according to an embodiment of the present invention comprises the steps of receiving digital information; And storing the received digital information using a pre-synthesized predetermined length DNA fragment corresponding to the first and second values of the digital data.

Furthermore, the DNA molecule-based information storage method according to an embodiment of the present invention further comprises the step of synthesizing the DNA fragments corresponding to the first value and the second value, wherein the DNA fragment has an electrical property of the DNA fragment It may include a predetermined sequence pattern corresponding to each of the first value and the second value to be different.

The storing may include storing the digital information using the DNA fragments by connecting the DNA fragments using a primer including a loop structure.

The storing may store the digital information by using electrical characteristics of the DNA sequence of the DNA fragment.

The DNA fragment has a start sequence pattern indicating the start of the DNA fragment, a value sequence pattern corresponding to any one of the first value and the second value, and an end indicating the end of the DNA fragment. Sequence patterns and linker sequence patterns for ligation assays.

The storing may additionally store encoding information for decoding in the DNA fragment.

Furthermore, the DNA molecule-based information storage method according to an embodiment of the present invention may further include amplifying the DNA fragment in which the digital information is stored using an amplification primer.

DNA molecule-based information storage device according to an embodiment of the present invention includes a receiving unit for receiving digital information; And a storage unit for storing the received digital information using a pre-synthesized DNA fragment of a predetermined length corresponding to the first value and the second value of the digital data.

Furthermore, the DNA molecule-based information storage device according to an embodiment of the present invention further comprises a synthesis unit for synthesizing the DNA fragments corresponding to the first value and the second value, wherein the DNA fragment has an electrical property of the DNA fragment It may include a predetermined sequence pattern corresponding to each of the first value and the second value to be different.

The storage unit may connect the DNA fragments by using a primer including a loop structure, thereby storing the digital information using the DNA fragments.

The storage unit may store the digital information by using electrical characteristics of the DNA sequence of the DNA fragment.

The DNA fragment has a start sequence pattern indicating the start of the DNA fragment, a value sequence pattern corresponding to any one of the first value and the second value, and an end indicating the end of the DNA fragment. Sequence patterns and linker sequence patterns for ligation assays.

The storage unit may additionally store encoding information for decoding in the DNA fragment.

Furthermore, the DNA molecule-based information storage device according to an embodiment of the present invention may further include an amplification unit for amplifying the DNA fragments stored with the digital information using an amplification primer.

According to embodiments of the present invention, digital information may be stored in a DNA molecule by using a DNA fragment including a sequence pattern preset in correspondence with a digital data value.

According to an embodiment of the present invention, since digital information can be stored using DNA fragments, it can be used as an information storage medium to supplement or replace a magnetic disk, and to store important information in a small space without electricity consumption in preparation for a fire or disaster. Can be stored safely.

According to an embodiment of the present invention, since digital information is stored using DNA fragments, it is robust to errors that may occur during synthesis, and digitally stored in DNA molecules using sequencing equipment, for example, nanopore sequencing equipment. Information can be read.

The present invention is a field of molecular computing that calculates the number of cases using the characteristics of DNA molecules that replicate itself, bioinformatics such as amplifying and storing DNA synthesized to store information or storing it in bacteria or the like (Bioinformatics). It can be applied to various fields such as digital information insertion for identifying a biostrain or virus with improved genome or virus identification, storage for information backup, and long-term storage of large data.

1 is a flowchart illustrating an operation of a method for storing DNA molecule-based information according to an embodiment of the present invention.

Figure 2 shows an example of comparing the existing method with the method according to the present invention.

Figure 3 shows an example for explaining the process of connecting the DNA fragments.

Figure 4 shows an example of storing the digital information using the Huffman code in the DNA molecule.

Figure 5 shows the configuration of the DNA molecule-based information storage device according to an embodiment of the present invention.

Advantages and features of the present invention, and methods for achieving them will be apparent with reference to the embodiments described below in detail in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various different forms, only the embodiments are to make the disclosure of the present invention complete, and common knowledge in the art to which the present invention pertains. It is provided to fully inform the person having the scope of the invention, and the invention is defined only by the scope of the claims.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In this specification, the singular also includes the plural unless specifically stated otherwise in the phrase. As used herein, “comprises” and / or “comprising” refers to a component, step, operation, and / or element that includes one or more other components, steps, operations, and / or elements. It does not exclude existence or addition.

Unless otherwise defined, all terms used in the present specification (including technical and scientific terms) may be used in a sense that can be commonly understood by those skilled in the art. In addition, terms that are defined beforehand that are generally used are not to be interpreted ideally or excessively unless they are clearly specifically defined.

Hereinafter, with reference to the accompanying drawings, it will be described in detail preferred embodiments of the present invention. The same reference numerals are used for the same elements in the drawings, and duplicate descriptions of the same elements are omitted.

Research into using DNA as an information storage medium has been tried continuously, and has a symbolic meaning in terms of reusing principles existing in nature. In the event of fire or disaster, important information can be used safely in a small space without electricity consumption, and can be used as an information storage medium to supplement or replace a commonly used magnetic disk. It can also be used in the field of molecular computing, which calculates the number of cases using the properties of DNA molecules that replicate themselves.

This field of DNA-based information storage is a field of bioinformatics, and is led by countries that have attempted biological attempts such as amplifying and storing DNA synthesized to store information or storing it in bacteria.

In addition, existing sequencing technologies are built on the silicon industry, where semiconductor technology is utilized, so information storage and reading using nanopores fabricated on silicon substrates may also be linked to the semiconductor industry.

Embodiments of the present invention, by storing the digital information in the DNA molecule using a DNA fragment containing a sequence pattern that is set in advance corresponding to the digital data value, to be used as an information storage medium to complement or replace the magnetic disk In the event of fire or disaster, important information can be safely stored in a small space without electricity consumption, and digital information is stored using DNA fragments, so it is robust to errors that may occur during synthesis, and sequencing equipment, for example, Nanopore sequencing equipment can be used to read digital information stored in DNA molecules.

Herein, the present invention may store digital information by using a DNA fragment of a predetermined length synthesized with respect to a first value of digital data, for example, 1 and a second value, for example, 0, and the DNA fragment may include a first fragment. A start sequence pattern comprising a value sequence pattern corresponding to the value or the second value, further indicating the beginning of the DNA fragment, an end sequence pattern indicating the end of the DNA fragment, and a ligation assay It may further comprise a linker sequence pattern for.

The linker sequence pattern can be placed to the left or right or front and back of the value sequence pattern, and the start sequence pattern and the end sequence pattern can be placed at the beginning and end of the DNA fragment.

Furthermore, the DNA fragments may be linked by primers including a loop structure, and digital information may be stored in the linked DNA fragments.

In addition, the present invention can store the digital information corresponding to the Huffman code with respect to the digital information stored in the DNA fragment, the information on the encoding, that is, the encoding information can be stored in addition to the DNA fragment.

Here, the encoding information is a signal indicating that the digital information starts in the DNA molecule (DNA sequence pattern), the total amount of digital information to be stored, information required to decode the digital information, and a signal indicating that the digital information recording portion of the DNA molecule ends (DNA sequence pattern). ), Information recording date, information recorder and the like may include additional information, the information included in the encoding information is not limited to the above-mentioned information, in the present invention includes all the information needed for encoding and decoding digital information Can be.

Furthermore, the present invention may store digital information in a DNA molecule including DNA fragments, and then amplify the DNA fragment in which the digital information is stored using an amplification primer.

The present invention synthesizes in advance a DNA fragment comprising a value sequence pattern corresponding to the first value and the second value, the value sequence pattern included in the DNA fragment may have different electrical properties. That is, the present invention can store the digital information of the first value or the second value in the DNA molecule by using the electrical properties of the DNA sequence of the DNA fragment.

This invention is demonstrated with reference to FIGS.

1 is a flowchart illustrating an operation of a method for storing DNA molecule-based information according to an embodiment of the present invention.

Referring to FIG. 1, the DNA molecule-based information storage method according to an embodiment of the present invention receives digital information and generates encoding information for use when decoding the received digital information (S110 and S120).

Here, step S110 may be represented as various types of digital data by a method of encoding digital information to be stored. For example, step S110 may be converted to 24 bits per Hangul Hangul when encoded by the UTF8 encoding scheme, and may be converted from at least 3 bits to Hangul 7 bits per Hangul Hangul when encoded by the Huffman code.

For example, in step S110, if "Chinese language" is encoded by Hoffman code as shown in FIG. 4A, "I" is converted into 7 bits of "0111100", and "D" is 6 of "100110". Is converted to 7 bits of "1110010", "is" is converted to 6 bits of "101100", "is" is converted to 4 bits of "0101", and "medium" is "1110111". Is converted to 7 bits of " station " to 7 bits of " 0111000 ", thereby receiving 44 bits of digital information for information " China "

The encoding information generated in step S120 is a signal that digital information starts in the DNA molecule (DNA sequence pattern), for example, a start sequence pattern, the total amount of digital information to be stored, information necessary for decoding digital information, and digital information in the DNA molecule. Signal that the recorded portion ends (DNA sequence pattern) For example, it may include additional information such as end sequence pattern, information recording date and information recorder, and may also include information about Huffman coding. That is, when storing digital information in the DNA molecule, step S120 may be a step of generating additional information necessary for restoration and storage integrity in addition to information to be recorded in the molecule.

When the digital information to be stored is received in step S110 and S120 and encoding information is received, the digital information and the encoding information are stored in a DNA fragment pre-synthesized for the first value and the second value, and the DNA fragments are concatenated. (S130, S140).

Here, the first value and the second value of the digital data may have different value sequence patterns that are preset so that the electrical properties of the DNA fragments are different.

For example, the digital data value 0 may have a sequence pattern of "TTTTTTTTTTTT", may have a sequence pattern of "TATT", the digital data value 1 may have a sequence pattern of "CCCGGGGCCC", or "ACCC" It may have a sequence pattern of. Of course, the sequence pattern of each of these digital data values may be determined by the operator or individual providing the technology and may be pre-synthesized to have different electrical properties for sensing or reading the digital information stored in the DNA molecule. That is, the sequence patterns of the first value and the second value of the digital data are pre-synthesized. When the digital information is received, the sequence pattern corresponding to the received digital information and the digital data bit values of the encoding information may be used as it is. have.

The DNA fragment in which the digital information is stored includes a start sequence pattern indicating the start of the DNA fragment, a value sequence pattern corresponding to one of the first value and the second value, and an end indicating the end of the DNA fragment. ) Sequence patterns and linker sequence patterns for ligation assays. For example, when the sequence pattern of the digital data value 0 is "TTTTTTTTTTTT", the DNA sequence segment consisting of the value sequence pattern of 0 and the linkers may be expressed as "GAGGTGAGGTGAGGTGAGGTTTTTTTTTTTAACTGAACTGAACTGAACTG", and the sequence pattern of the digital data value 1 is "CCCGGGGCCC The DNA sequence segment consisting of the value sequence pattern and linkers of 1 in case of "can be expressed as" GAGGTGAGGTGAGGTGAGGT CCCGGGGCCCAACTGAACTGAACTGAACTG ". Here, the sequence pattern for the linkers may have a random sequence, and may be a sequence linked to the front and back of the value sequence pattern or to the left and right. Of course, the value sequence pattern and the linker sequence pattern may vary depending on the situation, and the length may also vary depending on the situation.

The start sequence pattern and the end sequence pattern may also be preset, for example, the start sequence pattern may be 35C "CAGTCGCTCCACAAGTACCAGCCTCGTCTCCACAT" (start-5), or 39mer "TCAGATGTGGAGACGAGGCTGGTACTTGTGGAGCGACTG" (start-3), The end sequence pattern may be "ATCGGGATATGATTGAGCAAGCAATGGCAAGATTGACGG" (end-5) of 39mer or "CCGTCAATCTTGCCATTGCTTGCTCAATCATATCC" of 35mer. Of course, the start sequence pattern and the end sequence pattern in the present invention may vary depending on the situation, and its length may also vary.

In step S140, the process of linking the DNA fragments may be performed by repeatedly linking the DNA fragments to store a desired amount of digital information in the DNA molecule. As shown in FIG. 3A, two DNA fragments are sequentially connected. Paste to create a first DNA segment group, join two or more first segment groups in the desired order as shown in FIG. 3B to create a larger segment group, and group more fragments as shown in FIG. 3C. To give a property that can repeat the process of Figure 3a and 3b to give.

Herein, the DNA fragments used in FIG. 3A include a linker to enable sequence and ligation assays for expressing digital information 0 and 1, and a start adapter and an end adapter capable of connecting DNA fragments in order. End adapter) and the ligation sequence for fragment linkage in FIG. 3B may use a primer including a loop structure.

In addition, the method according to the invention amplifies a DNA fragment in which digital information is stored and connected by using an amplification primer, thereby storing a large number of DNA molecules (S150).

Here, although step S150 has been described as amplifying DNA molecules using an amplification primer, the present invention is not limited thereto, and the DNA molecules may be amplified by culturing microorganisms to be put in the plasma vector.

In this case, the amplification primer sequence may be set in advance, for example, the amplification primer sequence may be "5` CAGTCGCTCCACAAGTACCAGCCTCGTCTCCACAT 3`" or "5` CCGTCAATCTTGCCATTGCTTGCTCAATCATATCC 3`.

Of course, the present invention is not limited to the amplification of DNA molecules by amplification primers and plasma vectors, and any method capable of amplifying DNA molecules can be used.

Through this process, the method according to the present invention may store digital information in a DNA molecule by using a DNA fragment including a sequence pattern preset in correspondence with a digital data value.

In addition, since the method according to the present invention can store digital information by using DNA fragments, it can be used as an information storage medium to supplement or replace a magnetic disk, and to prepare important information in a small space without electricity consumption in case of fire or disaster. Can be stored safely.

According to an embodiment of the present invention, since digital information is stored using DNA fragments, it is robust to errors that may occur during synthesis, and digitally stored in DNA molecules using sequencing equipment, for example, nanopore sequencing equipment. Information can be read. That is, the digital information stored in the DNA molecule by the method of the present invention can recognize the difference in fragments of a specific length through the difference in the electrical properties of the context of the DNA sequence using nanopore sequencing equipment. Therefore, even if there are some errors in the synthesis, the digital data values can be correctly recognized.

For example, existing methods use two-bit digital data, such as 00 = A (adenine), 01 = T (thymine), 10 = G (guanine), 11 = C (cytosine), as shown in Figure 2a. As a method of expressing information, the digital information to be stored may be stored as the base "TAGCT" of DNA when "0100101101" is to be stored. However, this method can generate a synthesis error, cannot be stored in one molecule due to sequencing error, and can destroy a molecule during sequencing. On the other hand, the method according to the present invention is a method for storing digital information using a DNA fragment comprising a sequence pattern corresponding to digital data values 0 and 1, as shown in Figure 2b, the digital information to be stored "0100101101 In the case of ", by connecting the DNA fragments using DNA fragments for 0 and DNA fragments for 1, digital information can be stored using the DNA fragments.

Although digital data values 0 and 1 have been described to have different value sequence patterns in the present invention, the present invention is not limited thereto and may be pre-synthesized by the inclusion of a modified base. For example, a digital data value of 0 may consist of an unmodified base and have a sequence of 20 to 40 bp in length, and a digital data value of 1 may include a modulated base and a sequence of 20 to 40 bp in length. Can be. Here, the modified base may include inosine, 5-hydroxymethylcytosine, or the like.

As such, the method according to the present invention stores information based on the electrical characteristics of the DNA sequence, not the DNA sequence, so that digital information can be stored in one molecule using current synthesis and assay techniques. The sequencing may not destroy the molecule. That is, the method according to the present invention is robust to errors that may occur during synthesis because the information is stored by characterizing the electrical characteristics of the DNA sequence, not the DNA sequence, and the DNA fragment of the promise length when reading the stored digital information. It is robust against calling errors because it determines the overall electrical characteristics. For example, in order to read digital information stored in a DNA molecule, the digital data values 0 and 1 can be determined by detecting the electrical signal of the entire DNA fragment of a predetermined length. Of course, when the value sequence pattern is stored in the DNA fragments, the sequence pattern may be read using the difference of the electrical signal detected by the nanopore sequencing equipment, and the digital data values 1 and 0 may be determined through the digital pattern storage. When storing the digital data values 1 and 0 with the inclusion of the nucleotide base, the digital data value can be determined by detecting the electrical signal of the DNA fragment containing the modified base. The off may be set, and the cut off may be determined by an operator or an individual providing the corresponding technology, and the cut off may be determined through experiments or the like.

4 illustrates an example of storing digital information using a Huffman code in a DNA molecule. As shown in FIG. 4, FIG. 4 illustrates a case of storing "Chinese words of the country" encoded by the Huffman code in a DNA molecule. will be.

As shown in Fig. 4A, the digital information encoded by the Huffman code for the data "Chinese Words" to be stored is 44 bits of "01111001001101110010101100010111101110111000", and this 44 bits of digital information is a value sequence pattern for 0. It is stored in DNA fragments using "TATT" and the value sequence pattern "ACCC" for 1, and can be made up of a 817mer DNA sequence as shown in FIG. 4B by concatenating DNA fragments in which respective digital data values are stored. .

Here, the sequence patterns of a certain length arranged before and after or to the left and right of the value sequence patterns "TATT" and "ACCC" shown in FIG. 4B are linker sequence patterns, and the sequence pattern for the first predetermined length is the start sequence pattern, and the last The sequence pattern for a course may be an end sequence pattern. Since the linker sequence pattern is a random pattern with no information, when the digital information stored in a DNA molecule is read using a device such as a nanopore sequencing device, the start sequence pattern indicates the start of the digital information, and the value sequence pattern is determined. Through the digital data value stored, the end sequence pattern shows the end of the digital information, so the digital information stored in the DNA molecules. Of course, encoding information such as the Huffman code may also be stored, and by decoding the digital information stored in the DNA molecule through the stored encoding information, it is understood that the information stored in the DNA molecule is "Chinese language" as shown in FIG. 4B. Can be.

As described above, the method according to the present invention is a single DNA molecule-based information storage method that can be implemented even in the presence of an oligonucleotide synthesis error, and can store information in a DNA fragment having a specific length rather than a base in the DNA molecule. In addition, since the digital data values can be stored in the DNA fragments by arranging the sequences so that the electrical properties of the DNA fragments are different or by using a modified base, even if there are some errors in the synthesis, the electrical properties of the context of the DNA sequence The difference allows us to recognize the difference in the segments of a certain length.

In other words, the conventional method records information in a single base, so that even if one or two bases are damaged, the stored information can be damaged, while the present invention records information in a DNA fragment that is longer than the base. Even if the dog's base is damaged, the information itself is robust. In addition, the existing method overlaps the information so that it overlaps at least several hundred times per bit for accuracy. When the same number of molecules are used, the present invention may improve information accuracy than the existing method.

In addition, the present invention does not need to synthesize a new DNA sequence every time the digital information to be stored is different, and by connecting the DNA fragments expressing the synthesized 0 and 1 in the desired order, the digital information can be stored in the DNA molecule. have.

In addition, when storing digital information by the method of the present invention DNA DNA ㅇ

5 illustrates a configuration of a DNA molecule-based information storage device according to an embodiment of the present invention, and illustrates a configuration of an apparatus for performing the method described with reference to FIGS. 1 to 4.

Referring to FIG. 5, the DNA molecule-based information storage device 500 according to an embodiment of the present invention includes a receiver 510, a synthesizer 520, a storage 530, and an amplifier 540.

The receiver 510 receives digital information to be stored.

Here, the receiver 510 may encode the information to be stored by using a preset encoding scheme, for example, a Huffman code, and receive the encoded digital information.

The synthesis unit 520 synthesizes DNA fragments corresponding to the first value and the second value, which are digital data values.

Here, since the DNA fragments corresponding to the first value and the second value are pre-synthesized by the synthesis unit 520, the desired digital information may be stored using the DNA fragments corresponding to the digital data values.

Further, the DNA fragment synthesized by the synthesis unit 520 may include a start sequence pattern indicating the start of the DNA fragment, a value sequence pattern corresponding to any one of the first value and the second value, and the end of the DNA fragment. Linker sequence patterns for end sequence patterns and ligation assays.

The storage unit 530 stores the digital information received by the receiver 510 using a DNA fragment having a predetermined length corresponding to the first value and the second value.

Here, the storage unit 530 may store the digital information and the encoding information in the DNA fragments pre-synthesized with respect to the first value and the second value, and connect the DNA fragments.

The storage unit 530 connects the DNA fragments corresponding to each of the digital data value of the digital information and the digital data value of the encoding information by using a primer including a loop structure, and repeats the connection process, thereby encoding the digital information and encoding. Information can be stored in DNA molecules.

For example, the storage unit 530 joins two DNA fragments in sequence to generate a first DNA fragment group, and joins two or more first fragment groups in a desired order to generate a larger fragment group. By repeating, the desired amount of digital information can be stored in the DNA molecule.

The amplifying unit 540 amplifies DNA fragments or DNA molecules in which digital information is stored by culturing microorganisms to be put in amplification primers or plasma vectors.

Although the description of the apparatus of FIG. 5 is omitted, it will be apparent to those skilled in the art that the apparatus according to the present invention may include all the contents described in FIGS. 1 to 4.

In addition, the present invention may detect the digital information stored in the DNA molecule by the above-described method, and by confirming the sequence pattern for the digital data value through the difference in the electrical signal for the sequence of the DNA molecule, the digital stored in the DNA molecule The data stored in the DNA molecules can be identified by recognizing the data values and decoding the digital data. That is, the present invention is not limited to the method of storing digital data in the DNA molecule, and may also include a method of reading digital information stored in the DNA molecule in this manner. Specifically, the present invention can detect the digital signal stored in the sequence pattern of the DNA molecule through the same method as the nanopore sequencing method, and detect the sequence pattern by using the detected electrical signal difference, thereby detecting the stored digital data. have. Of course, the detection process detects the start sequence pattern in the DNA molecule, recognizes the beginning of storing the digital information, and confirms the value sequence pattern through the electrical signal difference for the DNA sequence after that portion, thereby saving the digital data stored in the DNA molecule. By recognizing the value and performing this process until the end sequence pattern is detected, digital data stored in the DNA molecule can be detected. The detected digital data may identify corresponding information by using a preset decoding scheme.

The system or apparatus described above may be implemented as a hardware component, a software component, and / or a combination of hardware components and software components. For example, the systems, devices, and components described in the embodiments may include, for example, processors, controllers, arithmetic logic units (ALUs), digital signal processors, microcomputers, field programmable arrays (FPAs). ), A programmable logic unit (PLU), a microprocessor, or any other device capable of executing and responding to instructions, may be implemented using one or more general purpose or special purpose computers. The processing device may execute an operating system (OS) and one or more software applications running on the operating system. The processing device may also access, store, manipulate, process, and generate data in response to the execution of the software. For convenience of explanation, one processing device may be described as being used, but one of ordinary skill in the art will appreciate that the processing device includes a plurality of processing elements and / or a plurality of types of processing elements. It can be seen that it may include. For example, the processing device may include a plurality of processors or one processor and one controller. In addition, other processing configurations are possible, such as parallel processors.

The software may include a computer program, code, instructions, or a combination of one or more of the above, and may configure the processing device to operate as desired, or process independently or collectively. You can command the device. Software and / or data may be any type of machine, component, physical device, virtual equipment, computer storage medium or device in order to be interpreted by or to provide instructions or data to the processing device. Or may be permanently or temporarily embodied in a signal wave to be transmitted. The software may be distributed over networked computer systems so that they may be stored or executed in a distributed manner. The software and data may be stored on one or more computer readable recording media.

The method according to the embodiments may be embodied in the form of program instructions that may be executed by various computer means and recorded on a computer readable medium. The computer readable medium may include program instructions, data files, data structures, and the like, alone or in combination. The program instructions recorded on the media may be those specially designed and constructed for the purposes of the embodiments, or they may be of the kind well-known and available to those having skill in the computer software arts. Examples of computer-readable recording media include magnetic media such as hard disks, floppy disks, and magnetic tape, optical media such as CD-ROMs, DVDs, and magnetic disks, such as floppy disks. Magneto-optical media, and hardware devices specifically configured to store and execute program instructions, such as ROM, RAM, flash memory, and the like. Examples of program instructions include not only machine code generated by a compiler, but also high-level language code that can be executed by a computer using an interpreter or the like. The hardware device described above may be configured to operate as one or more software modules to perform the operations of the embodiments, and vice versa.

Although the embodiments have been described by the limited embodiments and the drawings as described above, various modifications and variations are possible to those skilled in the art from the above description. For example, the described techniques may be performed in a different order than the described method, and / or components of the described systems, structures, devices, circuits, etc. may be combined or combined in a different form than the described method, or other components. Or, even if replaced or substituted by equivalents, an appropriate result can be achieved.

Therefore, other implementations, other embodiments, and equivalents to the claims are within the scope of the claims that follow.

Claims (14)

Receiving digital information; And Storing the received digital information using a pre-synthesized predetermined length DNA fragment corresponding to the first and second values of the digital data. DNA molecule-based information storage method comprising a. The method of claim 1, Synthesizing a DNA fragment corresponding to the first value and the second value More, The DNA fragment And a predetermined sequence pattern corresponding to each of the first value and the second value such that electrical properties of the DNA fragments are different. The method of claim 1, The storing step A DNA molecule-based information storage method, characterized in that for storing the digital information by using the DNA fragments by connecting the DNA fragments using a primer including a loop structure. The method of claim 1, The storing step And storing the digital information by using electrical characteristics of the DNA sequence of the DNA fragment. The method of claim 1, The DNA fragment A start sequence pattern indicating the start of the DNA fragment, a value sequence pattern corresponding to any one of the first value and the second value, an end sequence pattern indicating the end of the DNA fragment and A DNA molecule based information storage method comprising a linker sequence pattern for a ligation assay. The method of claim 1, The storing step And storing encoding information for decoding in the DNA fragment. The method of claim 1, Amplifying the DNA fragment storing the digital information using an amplification primer DNA molecule-based information storage method characterized in that it further comprises. A receiver for receiving digital information; And A storage unit for storing the received digital information using a pre-synthesized DNA fragment of a predetermined length corresponding to the first value and the second value of the digital data DNA molecule-based information storage device comprising a. The method of claim 8, Synthesis unit for synthesizing DNA fragments corresponding to the first value and the second value More, The DNA fragment And a predetermined sequence pattern corresponding to each of the first and second values so that the electrical properties of the DNA fragments are different. The method of claim 8, The storage unit A DNA molecule-based information storage device, characterized in that for storing the digital information by using the DNA fragments by connecting the DNA fragments using a primer (primer) comprising a loop structure. The method of claim 8, The storage unit The DNA molecule-based information storage device, characterized in that for storing the digital information by using the electrical properties of the DNA sequence of the DNA fragment. The method of claim 8, The DNA fragment A start sequence pattern indicating the start of the DNA fragment, a value sequence pattern corresponding to any one of the first value and the second value, an end sequence pattern indicating the end of the DNA fragment and A DNA molecule based information storage device comprising a linker sequence pattern for a ligation assay. The method of claim 8, The storage unit DNA molecule-based information storage device, characterized in that for additionally storing the encoding information for decoding in the DNA fragment. The method of claim 8, An amplification unit for amplifying the DNA fragment storing the digital information using an amplification primer DNA molecule-based information storage device further comprises.

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