WO2019230690A1 - Mosquito attractant, method for manufacturing mosquito attractant, and mosquito attractant composition - Google Patents

Abstract

The present invention addresses the problem of providing a mosquito attractant that exhibits high attractivity for both male mosquitoes and female mosquitoes. The present invention provides: a mosquito attractant having concentrated fruit juice having a Brix value of 15% or higher; a method for manufacturing a mosquito attractant, the method removing water from a fruit juice-containing liquid to increase the Brix value to 15% or higher; and a mosquito attractant composition comprising the mosquito attractant and an acrylate-based polymer.

Description

Mosquito attractant, method for producing mosquito attractant, and mosquito attractant composition

The present invention relates to a mosquito attractant, a method for producing a mosquito attractant, and a mosquito attractant composition, and more particularly, a mosquito attractant used for attracting, capturing or killing mosquitoes, and the mosquito The present invention relates to a method for producing an attractant and a mosquito attractant composition using the mosquito attractant.

蚊 When it is sucked by mosquitoes, itchy itch, and it causes rash and dermatitis at the site of blood sucking. In particular, some mosquitoes are considered to be hygienic and extremely harmful insects because they transmit pathogens such as dengue fever, zika fever, yellow fever, encephalitis, and malaria.

Mosquitoes include a thermal sensor that senses the body temperature of animals, a taste receptor that senses taste, an olfactory receptor that senses volatile substances such as body odor, and a carbon dioxide receptor that senses carbon dioxide, which is a highly volatile substance. Has an excellent chemoreception system and takes a variety of actions. For example, a female mosquito seeks blood before spawning, follows the carbon dioxide and body odor that the animal exhales by breathing, approaches the animal, senses the body temperature with a thermal sensor, detects the target animal, and sucks blood Are known. In addition, male mosquitoes that do not show blood-sucking behavior and female mosquitoes other than the spawning season feed on nectar, fruit juice, and sap to ingest sugar to ensure energy.

Conventionally, mosquitoes have been captured and killed using a trap, and various methods for attracting mosquitoes to the trap have been studied. For example, a mosquito trap that collects mosquitoes by utilizing the habit of generating carbon dioxide by a chemical reaction and collecting mosquitoes in carbon dioxide has been proposed (see Patent Document 1). In addition, the attractant is stored in the trap or adjacent to the trap, and the attracting component is gradually released into the air to obtain the attracting activity. As the attractant, vinegar and flavoring are used. A flying pest attractant containing an attracting substance as an active ingredient (see Patent Document 2), a mosquito attractant containing a mixture of lactic acid and methyl lactate as an active ingredient (see Patent Document 3), lysine, isovaleric acid, alanine, A mosquito attractant containing at least one of androstenol and sebum (see Patent Document 4) has been proposed.

Japanese Unexamined Patent Publication No. 2000-95623 Japanese Unexamined Patent Publication No. 2013-151470 Japanese Unexamined Patent Publication No. 6-65005 Japanese Patent Laid-Open No. 2002-241204

The method of attracting mosquitoes by generating carbon dioxide is effective for female mosquitoes, but is less attractive to male mosquitoes, and attractants that have been proposed in the past are sufficient. It cannot be said that it shows sex.
Therefore, the present invention is a mosquito attractant used for attracting, capturing or killing mosquitoes, and shows high attractability to both male and female mosquitoes. It is an object to provide an attractant.

As a result of intensive research, the present inventors have focused on increasing the attractiveness to mosquitoes by concentrating fruit juice, and concentrated fruit juice having a Brix value of 15% or more is obtained between male mosquitoes and female mosquitoes. The present inventors have found that the present invention exhibits excellent attractiveness in any case, and have completed the present invention.

That is, the present invention is characterized by the following (1) to (6).
(1) A mosquito attractant composed of concentrated fruit juice having a Brix value of 15% or more.
(2) The concentrated fruit juice is a fruit juice derived from at least one fruit selected from the group consisting of apple, orange, mandarin, oyster, fig, peach, grape, pear, pineapple and mango (1) The mosquito attractant described in 1.
(3) A method for producing a mosquito attractant comprising removing water from a fruit juice-containing liquid and setting the Brix value to 15% or more.
(4) The manufacturing method of the mosquito attractant as described in said (3) which removes the said water | moisture content by a boiling process.
(5) The fruit juice-containing liquid contains fruit juice derived from at least one fruit selected from the group consisting of apple, orange, mandarin orange, oyster, fig, peach, grape, pear, pineapple and mango (3) ) Or the method for producing a mosquito attractant according to (4).
(6) A mosquito attractant composition comprising the mosquito attractant of (1) or (2) and a water absorbent polymer, wherein the water absorbent polymer comprises an acrylate polymer.

Since the mosquito attractant of the present invention has excellent attractability to mosquitoes, mosquitoes can be captured or killed efficiently using the mosquito attractant of the present invention. In addition, the mosquito attractant composition of the present invention has excellent formulation stability when exposed to high temperatures and over time.

FIG. 1 is a perspective view showing a configuration of an olfactometer used in the embodiment.

Hereinafter, the mosquito attractant of the present invention, its production method, and the mosquito attractant composition will be described in detail.

(Mosquito attractant)
The mosquito attractant of this embodiment consists of concentrated fruit juice having a Brix value of 15% or more. Mosquito attractant consisting of concentrated fruit juice with a Brix value of 15% or more affects appetite, which is a common behavioral desire for both male and female mosquitoes. Can be attracted without distinction of mosquitoes.

Examples of fruit juices that can be used to attract mosquitoes include apples, oranges, mandarin oranges, lemons, grapefruits, yuzu, strawberries, peaches, grapes, pineapples, mangoes, melons, bananas, oysters, pears and figs. The fruit juice derived is mentioned, These 1 type may be used independently and may be used in combination of 2 or more type. Among these, fruit juices derived from at least one fruit selected from the group consisting of apples, oranges, mandarin oranges, oysters, figs, peaches, grapes, pears, pineapples and mangos are preferred, are readily available throughout the year and are inexpensive. From the viewpoint of being, apples, oranges, mandarin oranges and grapes are more preferable.

As the mosquito attractant of this embodiment, a concentrated fruit juice obtained by concentrating the above fruit juice is used. As the concentrated fruit juice, a fruit juice concentrated by a known method can be used. Examples thereof include concentrated fruit juice concentrated by heating, concentrated fruit juice concentrated by membrane filtration, concentrated fruit juice concentrated by freezing, and concentrated fruit juice concentrated under reduced pressure. Among them, concentrated fruit juice obtained by heat treatment such as concentrated fruit juice evaporated and concentrated by heating or concentrated fruit juice heated and concentrated under vacuum (also simply referred to as “heated fruit juice”) is an aroma component of fruit juice by heating. Since the content of components (such as linalool and pinene) that repel insects in many plants and changes, the attractiveness to mosquitoes can be further increased, which is preferable.

Concentrated fruit juice has a high Brix value because it has a high sugar concentration. The Brix value means the solution-soluble solid content concentration, and is the ratio of the weight (g) of soluble solids per 100 g of solution. The Brix value can be measured by a saccharimeter, a refractometer or the like. For example, as a commercially available sugar content meter, a pocket sugar content meter “PAL-1” (product number) manufactured by Atago Co., Ltd. can be used.

The Brix value of the mosquito attractant of this embodiment is 15% or more, preferably 20% or more, and more preferably 30% or more. When the Brix value is 15% or more, excellent attractability is exhibited for both male mosquitoes and female mosquitoes, and an attractive effect in a wide range can be obtained. The upper limit of the Brix value is not particularly limited, but is preferably 70% or less, and more preferably 60% or less.

(Manufacturing method of mosquito attractant)
The manufacturing method of the mosquito attractant of this embodiment includes removing the water | moisture content of the juice containing liquid containing desired fruit juice, and making Brix value 15% or more. Examples of the method for removing moisture from the juice-containing liquid include concentration methods such as 1) a heat concentration method (evaporation method), 2) a membrane concentration method, 3) a freeze concentration method, and 4) a vacuum concentration method. Hereinafter, a method for producing a mosquito attractant by the heat concentration method (evaporation method) will be described.

The heat concentration method (evaporation method) is a method of removing water by heating a fruit juice-containing liquid, that is, by boiling.
The juice-containing liquid is not particularly limited as long as it is a solution containing fruit juice derived from a desired fruit. Specifically, at least one kind of fruit juice obtained from fruits such as apple, orange, orange, lemon, grapefruit, yuzu, strawberry, peach, grape, pineapple, mango, melon, banana, oyster, pear, fig, etc. A containing solution can be used.

As the juice-containing liquid, fruit juice squeezed from fruits, commercially available fruit juices, commercially available fruit-derived jams, and the like can be used. In addition, since it will take a long time to concentrate until it becomes a predetermined Brix value when there is too little content of fruit juice, it is preferable that fruit juice containing liquid contains 30 weight% or more of fruit juice.

The means for heating the fruit juice-containing liquid is not particularly limited, and examples thereof include a method of boiling over a fire (boiling), a method of using a heating device such as a hot plate, an oven, and a heater. Among these, it is preferable to boil the juice-containing liquid by boiling. The juice-containing liquid is preferably heated with stirring in order to prevent the sugar contained in the fruit juice from being burnt, or to increase the heating efficiency by uniformly heating the juice-containing liquid.

The temperature condition for heating the fruit juice-containing liquid is preferably 70 to 90 ° C, more preferably 80 to 90 ° C. If the heating temperature is too low, the treatment takes too much time, which is economically undesirable. If the heating temperature is too high, the juice-containing liquid tends to burn and it is difficult to obtain a uniform concentrated fruit juice. By heating in the said temperature range, a water | moisture content can be efficiently evaporated, a burning can be suppressed, and uniform concentrated fruit juice can be obtained.

The heating time is not particularly limited as long as heating is performed until the Brix value of the fruit juice-containing liquid becomes 15% or more.

When the Brix value of the fruit juice-containing liquid reaches a desired value of 15% or more, the heat treatment is finished, and cooling may be performed by cooling or water cooling.

When concentrating with the heat concentration method, the aroma component of the juice contained in the juice-containing liquid changes due to the heating of the juice-containing liquid, and some mosquito attracting components increase or the components that inhibit mosquito attracting decrease. Therefore, it is estimated that the effect of attracting mosquitoes will be improved.

(Mosquito attractant-containing mixture)
The mosquito attractant of the present invention is a known attracting component, insecticidal component, antibacterial agent, preservative, coloring agent, solvent, ultraviolet absorber, retention agent, lubricant as long as it does not impair the mosquito attracting effect. It can be mixed with additives such as solvents, anti-corrosion inhibitors, antioxidants, surfactants, etc. to obtain a mosquito attractant-containing mixture.

The known attracting component is preferably a component that volatilizes at room temperature (20-30 ° C.). Examples of attracting ingredients include spirits such as brandy, whiskey, rum, vodka, shochu, and other sakes such as sake, wine (wine, cider), beer, shaoxing, etc .; black vinegar, red vinegar, Examples include brewed vinegars such as edible vinegar, apple vinegar and rice vinegar; sugars such as honey, liquid sugar and maple syrup; lactic acid products such as lactic acid beverages, yogurt and cheese; and fruity flavors. As the attracting component, one kind may be used alone, or two or more kinds may be used in combination. Among these, brewed liquor is preferred from the viewpoint of high attractability to mosquitoes and easy availability throughout the year.

Examples of the insecticidal component include dichlorvos, fenitrothion, IBTA, IBTE, transfluthrin, metflufluthrin, profluthrin, empentrin, propoxur, fenocarb, amidoflumet, dinotefuran, fipronil, hydramethylnon, carbaryl and the like.

Antibacterial agents or preservatives include, for example, chlorine dioxide, iodine, thymol, isopropylmethylphenol, formaldehyde, glutaraldehyde, ethanol, propyl alcohol, phenol, cresol, phenoxyethanol, cetylpyridinium chloride, potassium sorbate, sodium benzoate, benzoic acid Examples include acids, ε-polylysine, parabens and the like.

Examples of the colorant include blue No. 1 and yellow No. 4 synthetic colorants, natural pigments such as caramel pigments, and the like.

Examples of ultraviolet absorbers include trisresorcinol triazine compounds, benzotriazole compounds, hydroxyphenyl triazine compounds, and the like.

Examples of the solvent include organic solvents and water. Examples of the organic solvent include lower alcohols such as methanol, ethanol, propanol, 2-propanol, tert-butyl alcohol, ethylene glycol, and propylene glycol. Examples of water include distilled water, tap water, and deionized water. Can be mentioned.

Examples of the anti-fouling agent include denatonium benzoate and pepper extract.

Examples of the antioxidant include butylhydroxytoluene, green tea extract, tocopherol, sodium erythorbate, L-ascorbic acid and the like.

Examples of the surfactant include polyoxyethylene hydrogenated castor oil, glycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester and the like.

The mosquito attractant-containing mixture can be prepared by mixing and dissolving the mosquito attractant of the present invention and other components.

The content of the mosquito attractant contained in the mosquito attractant-containing mixture is not particularly limited as long as it does not impair the mosquito attracting effect, but is preferably 5 to 95% by weight, more preferably 10 to 95% by weight, 10 to 90% by weight is more preferable. The content of the mosquito attractant is preferably 5% by weight or more because the mosquito attractant can be continuously released until the end of use, and the content other than mosquito attractant is 95% by weight or less. Is preferable because it can be blended in an appropriate amount.

In addition, a non-volatile mosquito attractant that does not volatilize at room temperature (20 to 30 ° C.) so as not to impede the sustained release of the attractant and the liquid absorption of the mosquito attractant-containing composition as described below. The content in the containing mixture is preferably 0.1 to 60% by weight. When the content of the non-volatile component in the mosquito attractant-containing mixture exceeds 60% by weight, the non-volatile component is volatilized and the proportion of the non-volatile component retained on the carrier increases as the content decreases. Is likely to be hindered, the sustained release of the attracting component may not be maintained. The content of the nonvolatile component is more preferably 1 to 40% by weight.

(Mosquito attractant composition)
The mosquito attractant and the mosquito attractant-containing mixture of the present embodiment (hereinafter, the mosquito attractant and the mosquito attractant-containing mixture are also collectively referred to as “attractant liquid”) are in a liquid state, that is, a fluid state. However, a desired shape can be obtained in accordance with the usage form. For example, in addition to liquid, there may be mentioned a state of impregnation with a water-absorbing impregnation body such as paste, solid (jelly, powder, granular, etc.), volatile paper and the like. Specifically, by adding a gelling agent to a liquid attractant, impregnating a water-absorbing polymer with a liquid attractant, or carrying a liquid attractant on an inorganic carrier, paste-like or solid mosquitoes An attractant composition can be obtained.

Hereinafter, a mosquito attractant composition using a water-absorbing polymer will be described.

A water-absorbing polymer is a high-molecular material that swells and gels by absorbing water, and keeps the active ingredient inside by contacting it with a liquid containing a volatile active ingredient. The desired effect can be exhibited over a predetermined period.
As a preparation in which a volatile pest attracting component is retained in such a water-absorbing polymer, for example, in Japanese Patent Application Laid-Open No. 2012-143173, a liquid-absorbing polymer is added and the bulk density is 0.1 to A flying pest trapping tool comprising a drug carrier in which a liquid drug composition containing an insecticidal component and an attracting component is held in a wet state on a non-woven fabric carrier of 0.3 is described. No. 331625 describes a water-dispersed pheromone sustained-release preparation containing pheromone-containing polyacrylic acid copolymer particles and a pheromone release inhibitor.

Some attracting ingredients that attract mosquitoes include sugars and amino acids, and if the water-absorbing polymer retains the sugar- and amino acid-containing components, the water-absorbing properties will be increased when exposed to high temperatures or over time. The polymer may not maintain its shape and may lose its shape (specifically, the water-absorbing polymer dissolves and liquefies). If such a shape collapse occurs during the distribution of the preparation, the reliability of the product may be impaired, and if the shape collapse occurs during use, there may be a problem that the end of use cannot be confirmed.

Since the mosquito attractant of the present invention is obtained from fruit juice, it contains sugars and amino acids, but in the present invention, the above problems can be solved by using an acrylate polymer among water-absorbing polymers. .

The acrylate-based polymer has a repeating unit represented by the following formula (1) as a basic structure.

(In formula (1), M represents an alkali metal ion, and n is an integer representing the number of repeating units.)

Examples of the alkali metal ion of the acrylate polymer include sodium ion and potassium ion, and sodium ion is more preferable.

As the acrylate polymer, a sodium salt polymer is preferable because of its excellent transparency and easy availability. Examples of the acrylate polymer include a sodium acrylate copolymer and a crosslinked sodium salt of an acrylic acid polymer, and it is more preferable to use at least one of these.

In addition, the Maillard reaction by sugar and an amino acid arises by mix | blending sugar and an amino acid simultaneously. The Maillard reaction includes a redox reaction, and this redox reaction is presumed to have some influence on the structure of the water-absorbing polymer. However, the present inventors have found that, among water-absorbing polymers, acrylate-based polymers suppress the occurrence of shape loss even when an attracting component containing sugar and amino acid is retained, and the mechanism is not clear. However, it is considered that acrylate polymers are not easily affected by the oxidation-reduction reaction.

By containing an acrylate-based polymer as the water-absorbing polymer, the attracting liquid can be sufficiently absorbed by the water-absorbing polymer even when sugar and amino acids are contained in the attracting liquid. The water-absorbing polymer does not lose its shape when exposed to the bottom or with time. Furthermore, the end of use can be easily visually recognized with a decrease in the volume of the mosquito attractant composition.

When the water-absorbing polymer is impregnated with physiological saline at room temperature and normal pressure (25 ° C., 0.10 MPa), the water absorption capacity is 10 to 300 times its own weight, that is, 10 to 300 g per 1 g of the water-absorbing polymer. It preferably has the ability to absorb physiological saline, more preferably has a liquid absorption ratio of 20 to 200 times, and further preferably has a liquid absorption ratio of 50 to 150 times. When the liquid absorption ratio is 10 times or more, since the swelling property after absorbing the attracting liquid is large, more attracting components can be retained. Further, when the liquid absorption ratio is 300 times or less, the attractive component can be stably and gradually released after liquid absorption.

The water-absorbing polymer used in the mosquito attractant composition of this embodiment is preferably granular. The average particle diameter of the water-absorbing polymer in the dry state is preferably 50 μm to 20 mm, more preferably 100 μm to 10 mm, and even more preferably 150 μm to 5 mm. When the average particle size in the dry state is 50 μm or more, it can be uniformly dispersed in the attracting liquid and absorbed, and when it is 20 mm or less, the mosquito attractant composition is stored in the container. When used, the compositions, that is, the water-absorbing polymers swollen by the attracting liquid are less likely to form a gap, and the attracting component can be stably and gradually released.

The average particle size of the water-absorbing polymer (that is, the mosquito attractant composition) after absorbing the attractant is preferably 200 μm to 30 mm, more preferably 800 μm to 20 mm, and even more preferably 1000 μm to 15 mm. Because the average particle size of the mosquito attractant composition is 200 μm or more, the mosquito attractant composition stored in the container is not easily spilled even if the container is tilted, and is 30 mm or less. Since the gap is not easily formed and the density of the polymer in the container is stable, the attracting component is stably volatilized.

Water-absorbing polymers having different particle diameters in the dry state may be used in combination, but it is preferable to use those having a close particle diameter in order to make the absorption speed of the attractant liquid constant and the end of use. For example, the maximum particle size of the water-absorbing polymer is preferably 1.5 times or less, more preferably 1.2 times or less with respect to the average particle size, and the minimum particle size of the water-absorbing polymer is preferably The average particle diameter is preferably 0.3 times or more, more preferably 0.5 times or more.

The water-absorbing polymer is preferably used in the range of 0.1 to 30 parts by weight, more preferably 1 to 20 parts by weight, and even more preferably 5 to 10 parts by weight with respect to 100 parts by weight of the attracting liquid. By making the blend ratio of the water-absorbing polymer and the attracting liquid fall within the above range, the attracting liquid can be absorbed into the water-absorbing polymer without loss.

In the present embodiment, as the water-absorbing polymer, a water-absorbing polymer other than the polyacrylate polymer may be used as long as the maintenance of the shape as described above is not hindered.
Examples of other water-absorbing polymers include synthetic polymers such as polysulfonate-based polymers, maleic anhydride-based polymers, polyacrylamide-based polymers, and polyvinyl alcohol-based polymers, starch-based polymers, cellulose-based polymers, and polyalginic acid-based polymers. And polymers derived from natural products such as

The method for producing the mosquito attractant composition of the present embodiment using the water-absorbing polymer includes causing the water-absorbing polymer to absorb the attracting liquid. Examples of the method of absorbing the attracting liquid in the water-absorbing polymer include a method of immersing the water-absorbing polymer in the attracting liquid and causing the water-absorbing polymer to swell, dripping, spraying, and applying the attracting liquid to the water-absorbing polymer to impregnate the attracting liquid. Although the method of swelling etc. is mentioned, Since the method of immersing a water absorbing polymer in an attractant is simple, it is preferable.

The mosquito attractant composition of the present embodiment thus obtained preferably contains 5 to 70% by weight of sugar. By containing 5% by weight or more of sugar, the mosquito attracting effect can be exerted, and by containing 70% by weight or less, the attracting component can be stably and gradually released until the end of use.
The sugar content in the mosquito attractant composition can be measured as a Brix value with a saccharimeter (for example, a pocket saccharimeter “PAL-1” (product number) manufactured by Atago Co., Ltd.).

(Use (attraction) method of mosquito attractant and mosquito attractant composition)
The mosquito attractant and mosquito attractant composition of the present embodiment are installed together with the trap, for example, by filling a container having at least a part of the container opened. Mosquitoes attracted by the mosquito attractant or mosquito attractant composition of the present embodiment are captured by a trap.
Examples of places of use include living rooms, bedrooms, guest rooms, kitchens, entrances, and toilets, gardens, outdoors such as parks, and around pet huts.

As the catcher, a conventionally known one can be used, and a catcher equipped with an adhesive sheet, a catcher using a suction fan that sucks and captures mosquitoes by wind attraction, and the like can be mentioned.

Examples of the mosquitoes to be attracted in the present embodiment include squids such as Akaieka, Kodaka Aikaeka, Chikaeka, etc., Aedes albopictus such as Aedes albopictus, Aedes albopictus, etc. It is not limited.

Hereinafter, the effects of the mosquito attractant of the present invention will be described based on specific test examples, but the present invention is not limited to the following examples.

<Test Example 1>
Brix value of apple juice A (made by Nippon Fruit Processing Co., Ltd., trade name “50 ° BX transparent apple juice” (concentrated juice)) and apple juice B (produced by Kyojin Makiba Co., Ltd., trade name “Carefully selected domestic apple juice straight 100)” Was measured with a pocket sugar content meter “PAL-1” manufactured by Atago Co., Ltd. For each apple juice, the Brix value was measured three times based on the instruction manual, and the average value was determined. Apple juice A had a Brix value of 50.7%, and Apple juice B had a Brix value of 12.8%. Apple juice contains sugar and amino acids.

Next, the mosquito attraction contrast test of apple juice A and apple juice B was performed using the olfactometer method.
An olfactometer 10 as shown in FIG. 1 was used. The olfactometer 10 includes a pair of transparent specimen boxes (first specimen box 1A and second specimen box 1B), and a transparent cylindrical attraction connected to each of the first specimen box 1A and the second specimen box 1B. The parts 2A and 2B and the transparent mosquito storage box 3 connected to the attracting parts 2A and 2B are provided. The connecting part between the mosquito storage box 3 and the attracting parts 2A, 2B is provided with a check valve 4 that prevents the mosquitoes that have entered the attracting parts 2A, 2B from entering the attracting part 2A, 2B from entering the mosquito receiving box 3 again. ing. Clean air is allowed to flow through the olfactometer 10 to generate airflow at 0.3 m / s from the specimen boxes 1A and 1B toward the mosquito-containing box 3.

As sample 1, 20 mL of apple juice A was measured in a plastic cup (diameter 6 cm, depth 5 cm) and placed in the first sample box 1A of the olfactometer 10. Similarly, 20 mL of apple juice B was measured as a sample 2 in a plastic cup and placed in the second sample box 1B of the olfactometer 10.
Thirty male adults of Aedes albopictus were released in the mosquito housing box 3 and left for 16 hours. Mosquitoes attracted to the attracting part 2A connected to the first specimen box 1A and the attracting part 2B connected to the second specimen box 1B were counted. The test was performed 3 times, and the average value was obtained. The results are shown in Table 1.

<Test Example 2>
50 g of apple juice B used in Test Example 1 was measured, and 59 g of granulated sugar was added and dissolved to prepare apple juice C. When the Brix value of apple juice C was measured by a pocket sugar content meter “PAL-1” manufactured by Atago Co., Ltd., it was 53%, which is the upper limit of the sugar content detector, and was confirmed to have a Brix value of 53% or more.

Next, using the olfactometer method, a mosquito attraction comparison test was performed on specimen 1 (using apple juice A) used in Test Example 1 and specimen 3 using apple juice C prepared above.
The olfactometer 10 similar to that used in Test Example 1 was used for the attraction contrast test. In the same manner as in Test Example 1, an attraction test for Aedes albopictus was performed, and mosquitoes attracted to each attracting part were counted. The test was performed 3 times, and the average value was obtained. The results are shown in Table 2.

From the results of Test Examples 1 and 2, it was found that mosquitoes are easily attracted to apple juice A (Sample 1). In particular, from the results of Table 2, the attractiveness was not improved even when apple juice C (specimen 3) was prepared by adding granulated sugar to apple juice B having a low attractiveness to increase the Brix value. However, it was found that concentrated juice showed a high attractiveness.

<Test Example 3>
Apple juice D (prepared by Kirin Beverage Co., Ltd., trade name “Tropicana 100% Apple”, Brix value 11.0%) was boiled to prepare apple juice D. When the Brix value of apple juice D was measured with a pocket sugar content meter “PAL-1” manufactured by Atago Co., Ltd., the Brix value was 42.4%. Apple juice contains sugar and amino acids.

Next, using the olfactometer method, a mosquito attraction comparison test was performed on specimen 4 using apple juice D prepared above and specimen 5 using apple juice E.
The olfactometer 10 similar to that used in Test Example 1 was used for the attraction contrast test. In the same manner as in Test Example 1, an attraction test for Aedes albopictus was performed, and mosquitoes attracted to each attracting part were counted. The results are shown in Table 3.

From the results in Table 3, it was found that the mosquito attractability is enhanced by boiling and concentrating the unconcentrated apple juice E. From this result, it is presumed that by heating the fruit juice, the aroma component of the fruit juice changes and the attractiveness to mosquitoes increases.

<Test Example 4>
Orange fruit juice B (manufactured by Kirin Beverage Co., Ltd., trade name “Tropicana 100% Orange”, Brix value 11.2%) was boiled to prepare orange fruit juice A. When the Brix value of orange juice A was measured with a pocket sugar content meter “PAL-1” (product number) manufactured by Atago Co., Ltd., the Brix value was 47.8%. Orange juice contains sugar and amino acids.

Next, using the olfactometer method, the mosquito attraction comparison test was performed on the specimen 6 using the orange juice A prepared above and the specimen 7 using the orange juice B.
The olfactometer 10 similar to that used in Test Example 1 was used for the attraction contrast test. In the same manner as in Test Example 1, an attraction test for Aedes albopictus was performed, and mosquitoes attracted to each attracting part were counted. The results are shown in Table 4.

From the results in Table 4, it was found that the attractiveness of mosquitoes is enhanced by boiling and concentrating orange juice.

<Test Example 5>
Using the attracting liquid produced below, a change with time was confirmed when the water-absorbing polymer was impregnated with the attracting liquid.

1. Preparation of attractant liquid Apple juice A (produced by Nippon Fruit Processing Co., Ltd., trade name “50 ° BX apple transparent juice” (concentrated juice)), 50% by weight of Japanese sake (Takara Shuzo Co., Ltd., cooked sake, trade name “Sakana” ) Was mixed at a ratio of 50% by weight to prepare an attracting liquid. Note that apple juice and sake contain sugar and amino acids.

2. Preparation of mosquito attractant composition and test specimen (Sample 8)
100 g of the attracted liquid thus prepared was filled in a plastic cup (diameter φ8 cm, height 10 cm, capacity 200 mL). As a water-absorbing polymer, 5 g of an acrylic acid polymer partial sodium salt crosslinked product (trade name “Sunfresh ST-250”, average particle size: 510 μm, manufactured by SDP Global Co., Ltd.) is added to the water-absorbing polymer. Was impregnated to obtain a mosquito attractant composition.
The sugar content in the mosquito attractant composition was measured with a pocket sugar content meter “PAL-1” manufactured by Atago Co., Ltd. and found to be 31.1% by weight. Moreover, the average particle diameter of the mosquito attractant composition (water-absorbing polymer after liquid absorption) was 1.2 mm.
Thereafter, an aluminum film was thermally welded to the opening of the plastic cup, the inside of the cup was sealed, and a specimen 8 containing a mosquito attractant composition was obtained.

(Sample 9)
Sample 9 was the same as Sample 8 except that 10 g of a sodium acrylate copolymer (manufactured by AK ChemTech Co., Ltd., trade name “ASCO HISOBEAD”, average particle size: 2.8 to 4 mm) was used as the water-absorbing polymer. Was made.
In addition, the average particle diameter of the mosquito attractant composition (water-absorbing polymer after liquid absorption) was about 8 mm.

(Sample 10)
Specimen was the same as Specimen 8 except that 10 g of modified polyalkylene oxide (manufactured by Sumitomo Seika Co., Ltd., trade name “Aqua Coke TWB”, average particle size: 4 mm × 4 mm × 2 mm cuboid) was used as the water-absorbing polymer. 10 was produced.
The size of the mosquito attractant composition (water-absorbing polymer after liquid absorption) was 10 mm × 10 mm × 8 mm.

(Sample 11)
Sample 11 was prepared in the same manner as Sample 8, except that 5 g of a crosslinked isobutylene-maleic anhydride copolymer (Kuraray Co., Ltd., trade name “KI Gel-201K”, average particle size: 850 μm) was used as the water-absorbing polymer. Was made.
In addition, the average particle diameter of the mosquito attractant composition (water-absorbing polymer after liquid absorption) was 1.2 mm.

3. Test Each specimen was stored in a thermostat set at 40 ° C. for 3 months. The appearance of the specimen after storage was visually observed, and the change in shape and the presence or absence of browning were evaluated according to the following criteria.
Similarly, the specimen was stored in a thermostat set at 50 ° C. for 1 month, the appearance of the specimen after storage was visually observed, and the change in the appearance of the mosquito attractant composition was evaluated according to the following criteria.
The results are shown in Table 5.
[Evaluation criteria: Shape of mosquito attractant composition]
○: There is no change in the shape of the mosquito attractant composition compared to before storage.
X: The shape of the mosquito attractant composition is broken and liquefied.
[Evaluation criteria: Browning of mosquito attractant composition]
○: Browning of the mosquito attractant composition hardly occurs compared to before storage.
X: Browning so that a mosquito attractant composition can be judged visually.

From the results of Table 5, specimens 8 and 9 did not change with time, and the shape of the mosquito attractant composition could be maintained even at high temperature storage at 50 ° C. On the other hand, the specimen 10 did not change with time when stored at 40 ° C. for 3 months, but collapsed the water-absorbing polymer when stored at 50 ° C. for 1 month. And when the specimen 11 was stored at 40 ° C. for 3 months and stored at 50 ° C. for 1 month, the water-absorbing polymer was disintegrated and liquefied.
Therefore, the acrylate polymer can sufficiently absorb the attracting liquid containing the attracting component containing sugar and amino acid, and can gradually release the attracting component without causing the water-absorbing polymer to lose its shape over time. I understood.
Moreover, it was confirmed that the specimen 8 did not cause browning of the mosquito attractant composition during high-temperature storage, and the appearance of the mosquito attractant composition could be maintained well.

Here, the features of the embodiments of the mosquito attractant and the method for producing the mosquito attractant according to the present invention described above are briefly summarized in the following (1) to (11).
(1)
A mosquito attractant consisting of concentrated juice with a Brix value of 15% or more.
(2)
The concentrated juice is a juice derived from at least one fruit selected from the group consisting of apple, orange, mandarin orange, oyster, fig, peach, grape, pear, pineapple and mango. Mosquito attractant.
(3)
A method for producing a mosquito attractant comprising removing water from a fruit juice-containing liquid and setting the Brix value to 15% or more.
(4)
The manufacturing method of the mosquito attractant as described in said (3) which removes the said water | moisture content by a boiling process.
(5)
(3) or (3) above, wherein the juice-containing liquid comprises fruit juice derived from at least one fruit selected from the group consisting of apple, orange, mandarin, oyster, fig, peach, grape, pear, pineapple and mango. 4) A method for producing a mosquito attractant.
(6)
A mosquito attractant composition comprising the mosquito attractant of (1) or (2) and a water absorbent polymer, wherein the water absorbent polymer comprises an acrylate polymer.
(7)
The mosquito attractant composition according to (6), wherein the alkali metal ion of the acrylate polymer is sodium ion.
(8)
The mosquito attractant composition according to (7) above, wherein the acrylate polymer is at least one of a sodium acrylate copolymer and a crosslinked sodium salt of an acrylic acid polymer.
(9)
The mosquito attractant composition according to any one of (6) to (8), further comprising brewed liquor.
(10)
The mosquito attractant composition according to any one of the above (6) to (9), which contains 5 to 70% by weight of sugar.
(11)
The mosquito attractant composition according to any one of the above (6) to (10), wherein the water-absorbing polymer is in the form of granules having an average particle diameter of 50 μm to 20 mm in a dry state.

Although the present invention has been described in detail and with reference to specific embodiments, it will be apparent to those skilled in the art that various changes and modifications can be made without departing from the spirit and scope of the invention. This application is based on a Japanese patent application filed on May 29, 2018 (Japanese Patent Application No. 2018-102425) and a Japanese patent application filed on May 31, 2018 (Japanese Patent Application No. 2018-105381). Incorporated herein by reference.

1A 1st sample box 1B 2nd sample box 2A, 2B Attraction part 3 Mosquito accommodation box 4 Check valve 10 Olfactometer

Claims (6)

Mosquito attractant consisting of concentrated fruit juice with a Brix value of 15% or more. The mosquito according to claim 1, wherein the concentrated fruit juice is a fruit juice derived from at least one fruit selected from the group consisting of apple, orange, mandarin, oyster, fig, peach, grape, pear, pineapple and mango. An attractant. A method for producing a mosquito attractant, comprising removing moisture from a juice-containing liquid to make a Brix value 15% or more. The manufacturing method of the mosquito attractant of Claim 3 which removes the said water | moisture content by a simmering process. The fruit juice-containing liquid comprises fruit juice derived from at least one fruit selected from the group consisting of apple, orange, mandarin orange, oyster, fig, peach, grape, pear, pineapple and mango. The manufacturing method of mosquito attractant as described. A mosquito attractant composition comprising the mosquito attractant of claim 1 or 2 and a water absorbent polymer, wherein the water absorbent polymer comprises an acrylate polymer.

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