WO2019224837A1

WO2019224837A1 - A detox water formulation and method for preparing the same

Info

Publication number: WO2019224837A1
Application number: PCT/IN2019/050399
Authority: WO
Inventors: Sushil Kumar; Ram Singh; Mamta Choudhary
Original assignee: Individual
Current assignee: Individual
Priority date: 2018-05-24
Filing date: 2019-05-19
Publication date: 2019-11-28
Anticipated expiration: 2020-11-24

Abstract

Disclosed here, an effective natural detox concentrate and formulation thereof in a form of drinking water and a method for preparing the same. The said formulation comprises of an aqueous extract from a group of herbs selected from Terminalia chebula, Terminalia bellirica, Ocimum sanctum/ Ocimum tenuiflorum/Ocimum basilicum, Aegle marmelos, Citrus limon, and Aloe vera in a combination of two or more, wherein said formulation is maintained at alkaline pH by optimizing the water formulation and wherein said formulation is devoid of any preservative.

Description

A DETOX WATER FORMULATION AND METHOD FOR PREPARING THE SAME

Field of the Invention

This invention, in general relates to a formulation to detoxify the human body by removing the toxic elements therefrom. More particularly, the present invention provides an effective natural detox concentrate and formulation thereof in a form of drinking water and a method for preparing the same.

Background of the Invention

Every day we are exposed to environmental pollutants, preservatives, heavy metals, pesticides, food adulterants and many disease-causing chemicals including cancer and dementia. These toxins are ingested by our bodies and get stored in the tissues and cells throughout our bodies which interfere with optimal functioning of our organs. Further, our demanding and stressful lifestyle and unhealthy eating habits and schedule also add to toxic agents in our body. These agents negatively impact human health for by causing diseases and disorders like constipation, bloating, gas, headaches, fatigue, aches and pains, nausea, belly fat, skin problems, food cravings, low energy, bad breath, mood swings, obesity, produce body odor, allergies, yeast infection, acid reflux and heartburn, high cholesterol, kidney stones etc.

Various approaches have been disclosed in the prior arts in relation to work performed on the above issues. Some ways involve adding food supplements to daily diet that detoxes the human body and cleanses the liver.

Detox drinks are introduced in the market, which help reduce inflammation naturally, boost energy, support digestion, cleanse the liver and promote healthy skin. Detox drinks help to avoid toxic overload before it becomes a major health problem.

A 201 1 study published in the Journal of Alternative and Complementary Medicine assessed the use of clinical detoxification therapies by licensed naturopathic doctors in the United States. One hundred ninety-six naturopathic doctors completed a survey about the use of clinical detoxification therapies. 92 percent of respondents reported using detox therapies and over 75 percent utilized detoxification to treat patients for environmental exposures, general cleaning and preventive medicine, gastrointestinal disorders and autoimmune diseases. In comparison to adding specific food or clinical detoxification therapy, detox drinks are simple to make and more user-friendly. Detox drinks are prepared using various fruits, vegetables and herbs that stimulate detoxification while providing vitamins and minerals that keep the body functioning properly.

There are number of diffusion bottles available in the market which can be used to prepare detox drink employing different fruits, vegetables, herbs combination.

However, currently available solutions as disclosed above have their own challenges such as the complex preparation process, shelf life of the drink, sometime unpleasant smell, not user friendly.

Generally, a detox water preparation requires an infusion process which may take 2 to 6 hours or sometime overnight to infuse the water. Further, to avoid decomposition of fruits in the water and create undesired flavors, the fruits are removed from the drink.

The infused water must usually be consumed on the same day or, if refrigerated, within 3 days of infusion. Infused waters are meant to be consumed fresh. Infused water looks or smells bad after a few days; hence it cannot be consumed.

Taste of the infused water is another challenge. Some cases for better detox infusion require more time which makes the drink too bitter to even sip.

Use of water is another challenge to prepare a detox water. At different locations pH and constituents of water may vary, which can affect the efficacy of detox water. Too hot or too cold water can destroy the fragile enzymes and vitamins in fruit that are beneficial to health. Further, the presently available detox water can’t be kept at room temperature for more than 4-5 hours because it becomes a breeding ground for mold and bacteria.

Selection of Fruits is another challenge. There is a requirement of using fresh fruits only and that to organics ones, otherwise, it could potentially be adding pesticide residue along with nutrients, vitamins and antioxidants. Frozen fruits or dried fruits simply won’t give very good results or flavor.

Therefore, there still is an unmet need existing to provide a method or composition which enable to avoid drawback associated with prior arts and provide better results of the detox drink/water. Hence, it would be desirable to work on the alternative approach and provide an effective detox water composition and method for preparing the same.

Summary of the Invention

It is an objective of the present invention to overcome the limitations of the existing detox methodology/formulations, wherein the present invention provides an effective and user- friendly detox formulation which is in the form of natural or drinking water having selective herbal detoxifying agents along with required minerals in natural forms.

Another objective of the present invention is to provide a detox water formulation comprising a selective natural extract or synergistic combination of natural extracts which is effective to bind the toxic agents in the body and flush them out from the body.

Yet another objective of the present invention is to provide a detox water formulation wherein said water is good for detoxifying and hydrating the body by consuming less water than normal drinking water.

Yet another objective of the present invention is to provide a cost-effective methodology to develop a natural detox water without using chemical agents.

Yet another objective of the present invention is to provide a container to contain said detox water, wherein said container is uniquely designed and made by using good quality materials.

The above and other objectives are achieved in accordance with following embodiments; however, the present invention is not limited to the preferred embodiments described herein below.

In accordance with one preferred embodiment of the present invention, there is provided a synergistic detox water formulation comprising aqueous extract from a group of herbs selected from Terminalia chebula, Terminalia belleihca, Ocimum sanctum/ Ocimum tenuiflorum/ Ocimum basilicum, Aegle marmelos, Citrus limon, and Aloe vera in a combination of two or more, wherein said formulation is maintained at alkaline pH and wherein said formulation is devoid of any preservative. According to the present invention, alkaline pH is maintained by an alkalinizer and said alkalinizer is natural and of herbal origin adapted to achieve desired alkalinity of the water.

In accordance with one preferred embodiment of the present invention, there is provided a synergistic detox water formulation comprising aqueous extract from a group of herbs selected from Terminalia chebula, Terminalia bellirica, Ocimum sanctum/ Ocimum tenuiflorum/ Ocimum basilicum and their combination.

In accordance with one preferred embodiment of the present invention, there is provided a synergistic detox water formulation comprising aqueous extract from a group of herbs selected from Terminalia chebula, Terminalia bellirica, Ocimum sanctum / Ocimum tenuiflorum/ Ocimum basilicum in a combination, wherein said combination of aqueous herbal concentrate comprises Terminalia chebula, Terminalia bellirica, Ocimum sanctum / Ocimum tenuiflorum/ Ocimum basilicum in a ratio of about 2:1 to 1 :2.

In accordance with one preferred embodiment of the present invention, there is provided a synergistic detox water formulation comprising aqueous extract from a group of herbs selected from Terminalia chebula, Terminalia bellirica, Ocimum sanctum / Ocimum tenuiflorum/ Ocimum basilicum and a combination thereof, wherein said combination of aqueous herbal formulation is developed as a concentrate and further formulated in water formulation, wherein said herbal formulation is having an amount of about 5% to 95% by volume of Terminalia chebula or Terminalia bellirica or both; or an amount of about 5% to 95% by volume of Ocimum sanctum / Ocimum tenuiflorum/ Ocimum basilicum·, or an amount of about 5% to 95% by volume of Terminalia chebula or Terminalia bellirica or both, and an amount of about 5% to 95% by volume of Ocimum sanctum / Ocimum tenuiflorum/ Ocimum basilicum.

In accordance with one preferred embodiment of the present invention, there is provided a synergistic detox water formulation comprising aqueous extract from a group of herbs selected from Terminalia chebula, Terminalia bellirica, Ocimum sanctum / Ocimum tenuiflorum/ Ocimum basilicum in combination, wherein said combination of aqueous herbal formulation is developed as a concentrate and further formulated in water formulation, wherein said herbal formulation is having an amount of about 5% to 95% by volume of Terminalia chebula or Terminalia bellirica and an amount of about 5% to 95% by volume of Ocimum sanctum / Ocimum tenuiflorum/ Ocimum basilicum. In accordance with one preferred embodiment of the present invention, there is provided a synergistic detox water formulation comprising aqueous extract from a group of herbs selected from Terminalia chebula, Terminalia bellirica, Ocimum sanctum/ Ocimum tenuiflorum/ Ocimum basilicum in a combination, wherein said combination of aqueous herbal formulation developed as a concentrate and further formulated in water formulation, wherein said herbal formulation is having an amount of about 10% to 80% by volume of Terminalia chebula or Terminalia bellirica or both and an amount of about 10 to 80% by volume of Ocimum sanctum / Ocimum tenuiflorum/ Ocimum basilicum.

In accordance with one preferred embodiment of the present invention, there is provided a synergistic detox water formulation comprising aqueous extract from a group of herbs selected from Terminalia chebula, Terminalia bellirica, Ocimum sanctum / Ocimum tenuiflorum/ Ocimum basilicum in a combination, wherein said combination of aqueous herbal formulation is developed as a concentrate and further formulated in water formulation, wherein said herbal formulation is having an amount of about 20% to 40% by volume of Terminalia chebula or Terminalia bellirica or both and an amount of about 20 to 40% by volume of Ocimum sanctum / Ocimum tenuiflorum/ Ocimum basilicum.

In accordance with one preferred embodiment of the present invention, there is provided a synergistic detox water formulation comprising aqueous extract from a group of herbs selected from Terminalia chebula, Terminalia bellirica, Ocimum sanctum / Ocimum tenuiflorum/ Ocimum basilicum in a combination, wherein said combination of aqueous herbal formulation is developed as a concentrate and further formulated in water formulation, wherein said herbal formulation is having an amount of about 10% to 80% by volume of Terminalia chebula or Terminalia bellirica or both and an amount of about 10 to 80% by volume of Ocimum sanctum / Ocimum tenuiflorum/ Ocimum basilicum.

In accordance with one preferred embodiment of the present invention, there is provided a synergistic detox water formulation comprising aqueous extract from a group of herbs selected from Terminalia chebula, Terminalia bellirica, Ocimum sanctum/ Ocimum tenuiflorum/ Ocimum basilicum, Aegle marmelos, Citrus limon, and Aloe vera in a combination of two or more, wherein said formulation is maintained at alkaline pH by optimizing the herbal formulation and water and wherein said formulation is devoid of any preservative, wherein optimization of pH is achieved by employing sea salt or natural alkalinizer. In accordance with one preferred embodiment of the present invention, there is provided a synergistic detox water formulation comprising aqueous extract of a group of herbs, comprising an amount of about 5% by volume Terminalia chebula, about 5% by volume Terminalia bellirica, about 5% by volume Aegle marmelos, about 5% by volume Citrus lirnon in a combination of two or more.

In accordance with one preferred embodiment of the present invention, there is provided a synergistic detox water formulation comprising aqueous extract from a group of herbs selected from Terminalia chebula, Terminalia bellirica, Aegle marmelos, Citrus lirnon and Aloe vera in a combination of two or more along with sea salt or natural alkalinizer.

In accordance with one preferred embodiment of the present invention, there is provided a synergistic detox water formulation comprising aqueous extract from a group of herbs selected from Terminalia chebula, Terminalia bellirica, Aegle marmelos, Citrus lirnon, and Aloe vera in a combination of two or more along with sea salt or natural alkalinizer, wherein said combination of aqueous herbal formulation is developed as a concentrate and further formulated in water formulation, wherein said herbal formulation is having an amount of about 5% by volume of Terminalia chebula, about 5% by volume of Terminalia bellirica, about 5% by volume of Aegle marmelos, about 5% by volume of Citrus lirnon, and about 5% by volume of Aloe vera in a combination of two or more.

According to the present invention, said formulation is having pH in a range of 7.2-7.9 and long self-life or shelf life of up to 6 months to 1 year.

According to the present invention, said water formulation is having total dissolved solutes (TDS) in a range of 80-150.

According to the present invention, wherein said formulation is having active ingredients which are capable of providing antioxidant effect of more than 16 times than the normal drinking water.

According to the present invention, wherein said formulation are having active ingredients which are capable of providing detoxification of the liver by 20-fold or more.

According to the present invention, wherein said herbal powder/extracts is obtained from any desired part of the plant/herbs/fruits, preferably fruits and more preferably large size ripe fruits. According to the present invention, wherein said formulation further comprises one or more of neutraceutically acceptable or food grade excipients and wherein said formulation is further formulated in any suitable oral formulation including tablet, capsule, chewing gum, candy lozenges, granules, powder or any liquid Forms and the like, wherein said liquid forms may include juices, soft drink, beverages etc.

According to the present invention, wherein said water formulation further comprises one or more flavoring agents.

According to an embodiment of the present invention, the water formulation is prepared by a method comprising extracting all or selected one or more plant parts of herbs by aqueous extraction, wherein the herbs used in concentrates are identified, isolated, extracted and characterized for the effective constituents present in said herbs, filtering the one or more plant extract, concentrating the plant extract to achieve aqueous concentrate of selected plants at optimum temperature and pH, blending the one or more plant aqueous concentrate to achieve a desired pH and stability of the herbal concentrate, further, synergistically blending with natural antioxidants to achieve homogeneously clear concentrate, and further developing the water formulation, wherein the resultant water formulation is having desired pH value within 6.5-8.5, falling within EPA standards.

In accordance with one embodiment of the present invention, there is provided a method for preparing the herbal detox water formulation, wherein the method comprising extracting all or selected one or more plant parts of herbs by aqueous extraction, wherein the herbs used in concentrates are identified, isolated, extracted and characterized for the effective constituents present in said herbs, filtering the one or more plant extract, concentrating the plant extract to achieve aqueous concentrate of selected plants at optimum temperature and pH, blending the one or more plant aqueous concentrate to achieve a desired pH and stability of the herbal concentrate, further, synergistically blending with natural antioxidants to achieve homogeneously clear concentrate, and further developing the water formulation, wherein the resultant water formulation is having desired pH value of 7.2-7.9.

These and other embodiments, processes and features of the invention will become more fully apparent when the following detailed description is read with the accompanying experiments. However, both the foregoing summary of the invention and the following detailed description of it as presented herein are understood to be illustrative of the present invention and not restrictive thereof and are non-limiting with respect to the scope of the invention.

Brief Description of Drawing Figures

To illustrate the solutions according to the embodiments of the present disclosure more clearly, the accompanying drawings needed for describing the embodiments are introduced below briefly. Apparently, the accompanying drawings in the following descriptions merely show some of the embodiments of the present disclosure, and persons skilled in the art may obtain other drawings according to the accompanying drawings without creative efforts.

Figure-1 : illustrating schematic graphical representation of antioxidant activity of test substances against Hydrogen peroxide induced toxification.

Figure-2: Bar graph illustrating antioxidant activity of Liquid sample in HepG2 cells pmole Glutathione/mg protein in HepG2 cells after treatment with Liquid sample in Hydrogen peroxide intoxicated HEPG-2 cells

Figure-3: Bar graph illustrating in vitro cytotoxicity studies on human glial cell line by MTT assay

Figure-4: Bar graph of densitometric analysis of gene transcripts, the relative level of CYP3A4 gene expression is normalized to GAPDH. Values shown depict arbitrary units Figure-5: RT-PCR profile of CYP3A4 gene amplified from drug treated HEp-G2 cell Line

Description of the Invention

In view of the foregoing disadvantages inherent in the known types of existing detox methodology/formulations in the prior art, the present invention provides an effective and user-friendly detox formulation which is in the form of natural water having selective herbal detoxifying agents along with required minerals in natural forms which synergistically overcomes the various drawbacks existing in the art.

Further, there has thus been broadly outlined the more important features of the invention in order to better understanding of the advantage and in order to better appreciation of contribution of present invention than the existing art.

The general purpose of the present invention, which is described subsequently in greater detail, is to provide an effective detox formulation and methods for making the same, wherein the invention provides an innovative detox formulation having selective herbal detoxifying agents along with required minerals in natural forms and method thereof is not anticipated, obvious, taught or suggested, or even implied by the prior art, either alone or in any combination thereof.

The solutions of the present disclosure are clearly described in the following with reference to the accompanying experimental supporting analysis and data. It is obvious that the embodiments to be described are only a part rather than all the embodiments of the present disclosure. All other embodiments obtained by persons skilled in the art based on the embodiments of the present disclosure without creative efforts shall fall within the protection scope of the present disclosure.

The numerous specific details in the description are set forth in order to provide a thorough understanding of embodiments of the present invention. It will be apparent to one skilled in the art that embodiments of the present invention may be practiced without some of these specific details.

Various terms as used herein in the invention is not defined and it should be given the broadest definition persons in the pertinent art have given that term as reflected in printed publications and issued patents at the time of filing.

“Herbal detox formulation” in accordance with the present invention means the formulation is prepared by using selective herbs and formulated into a herbal concentrate which can be further developed in water formulation which elicit desired detoxification effect of selected herbs. Further the term‘formulation’ or‘solution’ or‘composition’ are interchangeably used in the specification.

Unless otherwise stated all weight percentages as mentioned herein are based on the total weight or volume of the composition.

It must be noted that, as used in the specification and the appended claims, the singular forms‘a’,‘an’ and‘the’ include plural referents unless the context clearly dictates otherwise.

The term‘about’ is used synonymously with the term‘approximately’. Illustratively, the use of a term‘about’ indicates and includes the values slightly outside the cited values i.e. plus or minus 0.1 to 10 %, which are also effective and safe. According to one non-limiting embodiment of the present invention, the present disclosure relates to a herbal concentrate, which is used to prepare a detox water formulation, wherein said herbal concentrate is prepared by identifying a selective herb, extracted and characterized the effective components in the herb, removing of undesired components while retaining desired and effective components, developing the concentrate by an innovative method by employing the desired amount of herbal extracts with aqueous media without adding any preservative.

In accordance with another embodiment of the present invention, there is provided a method to prepare detox concentrate formulation wherein said method comprises selecting the herbs and identifying the desired characteristics of said herbs, extracting the crude extract from the selected plant part using a solvent system in an effective ratio, preferably aqueous system, identifying and removing the undesired components, homogenously mixing the aqueous extract in aqueous medium by using on-off technique to develop a clear solution, intermittently adding selective natural antioxidants to enhance the detox effect of the concentrate.

In accordance with another embodiment of the present invention, there is provided a method to prepare detox concentrate formulation wherein said method comprises selecting the herbs and identifying the desired characteristics of said herbs, extracting the crude extract from the selected plant part using a solvent system in an effective ratio, preferably aqueous system, identifying and removing the undesired components, homogenously mixing the aqueous extract in aqueous medium by using on-off technique to develop a clear solution, intermittently adding selective natural antioxidants to enhance the detox effect of the concentrate, wherein suitable antioxidants are selected from a group of alkaline herbs, plants or fruits and wherein said formulation is having selective range of herbs and alkaline herbs/plant/fruits extract to enhance the detox effect of more than 95% with respect to antioxidant activity.

In accordance with one preferred embodiment of the present invention, there is provided a synergistic detox water formulation comprising aqueous extract from a group of herbs selected from Terminalia chebula, Terminalia belleihca, Ocimum sanctum/ Ocimum tenuiflorum/ Ocimum basilicum, Aegle marmelos, Citrus limon, and Aloe vera in a combination of two or more, wherein said formulation is maintained at alkaline pH by optimizing the herbal formulation and water and wherein said formulation is devoid of any preservative. The qualitative and quantitative studies of the concentrate and water formulation thereof have been done after proper selection of solvent system having aqueous environment and extraction method to give better output. The stability with respect to pH, chemical structure and antioxidant property of the concentrate have been optimized to make it suitable to act as detox material in drinking water without changing the colour and taste of the drinking water. According to the invention, proportionate use of herbal extract in detox concentrate imparting useful and synergistic characteristic include stable pH of the concentrate, effective detoxification of liver in detoxification process, better antioxidant than normal drinking water. The ratio of different herbal extract is pivotal on pH stability, antioxidant effect and detoxification effect.

In accordance with one other embodiment of the present invention, there is provided a detox water formulation comprising an aqueous concentrate having an herbal extract wherein the herbal extract is obtained by selecting herbs from a species of Terminalia, Citrus, Ocimum and Aloe preferably Terminalia chebula in a desired quantity and wherein the extract is obtained by analyzing selective varieties of Terminalia chebula and wherein concentrate is further synergistically comprises the natural antioxidants.

In accordance with one other embodiment of the present invention, there is provided a detox water formulation, wherein said formulation is having alginate, glycosides, coumarin conjugated with gallic acid, chebulosides as well as other phenolic compounds including ellagic acid, 2,4-chebulyl^-D-glucopyranose, chebulinic acid, gallic acid, ethyl gallate, punicalagin, terflavin A, terchebin, luteolin, and tannic acid. In a desired ratio which are effective to use for detoxifying and cleansing a body, particularly a human body.

According to the present invention, there are other natural antioxidants that are added synergistically to enhance the effect of said detox water, wherein said natural antioxidants are selected from a group comprising carotenoids, vitamin C, vitamin E, vitamin A, turmeric, ginger, rosemary extract, carrot, cucumber, berries etc. However, the addition of said natural oxidants are not limited to the disclosed antioxidants according to the present application.

The extract is aqueous extract of detoxifying agents and obtained from any desired part of the plant/herbs/fruits. Preferably fruits and more preferably large size ripe fruits are used for extraction.

According to the present invention, the detox water composition prepared herein is having an unique characteristic of binding and holding the bound toxins within the matrix, and has an effective detoxifying effect while requiring an appreciably smaller amount of the concentrate formulation/composition to be used, thus reducing undesirable side effects associated with the use of detoxifying agents.

According to the present invention, the detox water formulation prepared herein is completely natural and comprises of herbal concentrate along with other natural antioxidants and wherein the herbs used in concentrates are identified, isolated, extracted and characterized the effective constituent present in said herbs and wherein said constituents are further synergistically blended with natural antioxidants to achieve homogeneously clear concentrate. The resultant water is having desired pH value within 6.5-8.5, which falls within EPA standards. More preferably the pH of the water formulation is towards alkaline and good for human health. Further the resultant water formulation can be stored at room temperature.

The extracts used in the present invention is extracted by employing any parts of the herbs, preferably fruits, most preferably a specific variety of fruits.

The method of preparing the detox concentrate and water formulation thereof is unique, which enhance the effect of detoxifying agents via natural process.

The detox water according to the present invention is effective to strengthen the digestive system and the immune system and renewed internal energy with pure and clean body. It also provides clear and light mind.

According to the present invention, the container used in storing the detox water is preferably BPA free excellent quality plastic however it is not limited and can be developed by using any other desired metallic materials such as to any other desired container selected from the containers made by glass, steel, copper, brass etc.

According to the present invention, there is provided a detox water formulation which is very effective to remove toxins from the body and cleanse the liver, reduces disease-causing inflammation and swelling within the body, promote skin health, help to boost the metabolism, energy and mental alertness and also help in weight loss.

The detox formulation according to the present invention is able to solve the drawback associated with prior known detox formulations and methodology by avoiding freezing, time consuming and complex process for preparation, pungent smell if kept for longtime etc. The aqueous extract prepared using the isolation, extraction and purification methods has been evaluated for the presence or absence of different groups of compounds employing by available testing methods.

Below are some of exemplary embodiments, wherein detox formulation is prepared by using various aqueous extract in various synergistic ratio and employed for various test to analyze antioxidant and detox effect of said formulation.

Example 1

Blending homogenously an aqueous extract of fruits of herbs of Terminalia chebula and herbs Ocimum sanctum/ Ocimum tenuiflorum/ Ocimum basilicum in a ratio of 1 :1 , 1 :2, 2:1 , adding an UV radiated water and further increasing the percentage range of Ocimum sanctum to optimize the pH towards alkaline.

Example 2

Blending homogenously an aqueous extract of fruits of herbs of Terminalia chebula and herbs Ocimum sanctum / Ocimum tenuiflorum/ Ocimum basilicum, Aegle marmelos, Citrus limon, and Aloe vera in a ratio of 1 :1 :1 :1 :1 , 1 :2:1 :1 :2, 2:1 :1 :2:1 , adding an UV radiated water and further increasing the percentage range of Ocimum sanctum to optimize the pH towards alkaline.

Example 3

Referring Figure 1 , the resultant concentrate is formulated as a water formulation according to example above and tested for in vitro cytotoxicity studies against Human Hepatocytes cell line by MTT assay exposing the cells to different concentrations of test substance (1000 pg/ml to 62.5 pg/ml), and showed moderate toxicity. It clearly demonstrates that the liquid sample at 1000pg/ml have protective role against oxidative damage in the liver cells as shown in the figure below.

Example 4

Referring Figure 2, the resultant concentrate is formulated as a water formulation according to example above and tested for in vitro anti-oxidant activity of test substance was evaluated in human hepatocytes cell line. Test substance showed In vitro anti-oxidant activity by modulating GSH levels in dose dependent manner, where liquid sample at a concentration of 1000pg/ml significantly increased activities of GR compared to untreated cells, by elevating the levels of glutathione with 24.56 and 19.10 mM per mg of protein at test doses 1000 and 500 pg/ml, respectively.

Glutathione, a major non-protein thiol in living organisms, plays a central role in coordinating the body’s antioxidant defense processes. Excessive peroxidation causes increased glutathione consumption. Therefore, it clearly demonstrates that the liquid sample at 1000pg/ml have protective role against oxidative damage in the liver cells.

Example 5

Referring Figure 3, the resultant concentrate is formulated as a water formulation according to example above and tested for in vitro cytotoxicity studies on human glial cell line by MTT assay exposing the cells to different concentrations of test substance (1000 pg/ml to 62.5 pg/ml) and showed no toxicity. Wherein Cytoprotective activity of liquid sample against hydrogen peroxide induced toxicity on U-87 cell. The U-87 cells were cultured in 96 wells (1 x 104 cell/well in 10 mL of complete Ham F-12), for 24 hours, and pre-treated with liquid sample at the non-toxic concentrations for 24 hours prior to undergoing incubation with H202. Cell viability was then determined by MTT assay and expressed as the mean ± standard deviation (n = 3). So the two non-toxic concentrations (1000 pg/ml and 500 pg/ml) were taken for cytoprotective studies. In cytoprotetcive study test substance showed significant efficacy by offering 62.77±4.7and 31 .54±3.9 % protection against hydrogen peroxide induced toxicity at 1000 and 500 pg/ml, respectively.

Example 6

Referring figure 4 and figure 5, the resultant concentrate is formulated as a water formulation according to example above and tested for in vitro cytotoxicity studies on human hepatoma cell line by MTT assay exposing the cells to different concentrations of test substance (1000 pg/ml to 62.5 pg/ml) and showed no toxicity. So the two non-toxic concentrations (1000 pg/ml and 500 pg/ml) were taken for gene expression study. In cytochrome p450 modulatory study the test substance was evaluated for its In vitro Gene Expression activity in human hepatoma cell line. It showed significant efficacy in Cytochrome 3A4. The level of mRNA expression of different proteins involved in cell cycle regulation analyzed by reverse transcriptase PCR, test substance at 1000 pg/ml and 500 pg/ml showed up regulation on the levels of cytochrome p450-3A4 gene expression by 0.20 and 0.17 fold respectively as compared to control. In brief liquid sample regulated Cyp3A4 gene expression that shows it’s predominantly expressed in the Human Hepatoma cell. Based on the description of disclosed embodiments, persons skilled in the art can implement or apply the present disclosure. Various modifications of the embodiments are apparent to persons skilled in the art, and general principles defined in the specification can be implemented in other embodiments without departing from the spirit or scope of the present disclosure. Therefore, the present disclosure is not limited to the embodiments in the specification but intends to cover the most extensive scope consistent with the principle and the novel features disclosed in the specification.

Claims

We Claim:

1. A synergistic detox water formulation comprising aqueous extract from a group of herbs selected from Terminalia chebula, Terminalia bellirica, Ocimum sanctum / Ocimum tenuiflorum/ Ocimum basilicum, Aegle marmelos, Citrus limon, and Aloe vera in a combination of two or more, wherein said formulation is maintained at alkaline pH and wherein said formulation is devoid of any preservative.

2. The herbal detox water formulation as claimed in claim 1 , wherein alkaline pH is maintained by an alkalinizer, wherein said alkaliinizer is natural or of herbal origin adapted to achieve desired alkalinity of the water.

3. The herbal detox water formulation as claimed in claim 1 , wherein said formulation comprises an effective quantity of aqueous extract of the herbs selected from Terminalia chebula, Terminalia bellirica, Ocimum sanctum/ Ocimum tenuiflorum/Ocimum basilicum and a combination.

4. The herbal detox water formulation as claimed in claim 1 , wherein said combination of aqueous herbal formulation is developed as a concentrate and further formulated in water formulation, said water formulation comprising Terminalia chebula, Terminalia bellirica, Ocimum sanctum/ Ocimum tenuiflorum/Ocimum basilicum in a ratio of about 2:1 to 1 :2.

5. The herbal detox water formulation as claimed in claim 1 , wherein said combination of aqueous herbal formulation is developed as a concentrate and further formulated in water formulation, wherein said water formulation is having an amount about 5% to 95% of Terminalia chebula or Terminalia bellirica and an amount about 5% to 95% of Ocimum sanctum/ Ocimum tenuiflorum/Ocimum basilicum.

6. The herbal detox water formulation as claimed in claim 1 , wherein said combination of aqueous herbal formulation developed as a concentrate and further formulated in water formulation, wherein said herbal formulation is further comprising sea salt or natural alkalinizer.

7. The herbal detox water formulation as claimed in claim 1 , wherein said formulation comprising an effective quantity of aqueous extract of the herbs selected from Terminalia chebula, Terminalia bellirica, Aegle marmelos, Citrus limon in a combination of two or more along with sea salt or natural alkalinizer.

8. The herbal detox water formulation as claimed in claim 7, wherein said formulation comprising an amount of about 5% Terminalia chebula, about 5% Terminalia bellirica, about 5% Aegle marmelos, about 5% Citrus Umon in a combination of two or more.

9. The herbal detox water formulation as claimed in claim 1 , wherein said formulation comprising an effective quantity of aqueous extract of the herbs selected from Terminalia chebula, Terminalia bellirica, Aegle marmelos, Citrus limon, and Aloe vera in a combination of two or more along with sea salt or natural alkalinizer.

10. The herbal detox water formulation as claimed in claim 9, wherein said formulation comprising an amount of about 5% Terminalia chebula, about 5% Terminalia bellirica, about 5% Aegle marmelos, about 5% Citrus limon, and about 5% Aloe vera in a combination of two or more.

1 1 . The herbal detox water formulation as claimed in claim 1 , wherein said formulation is having long self-life up to 6 months to 1 year.

12. The herbal detox water formulation as claimed in claim 1 , wherein said formulation is having pH range of 7.2-7.9.

13. The herbal detox water formulation as claimed in claim 1 , wherein said formulation is having TDS in a range of 80-150.

14. The herbal detox water formulation as claimed in claim 1 , wherein said formulation is having active ingredients which are capable of providing antioxidant effect of more than 16 times than the normal drinking water.

15. The herbal detox water formulation as claimed in claim 1 , wherein said formulation are having active ingredients which are capable of providing detoxification of the liver by 20 fold or more.

16. The herbal detox water formulation as claimed in claim 1 , wherein said extracts is obtained from any desired part of the plant/herbs/fruits, preferably fruits and more preferably large size ripe fruits.

17. The herbal detox water formulation as claimed in claim 1 , wherein said formulation further comprises one or more of a neutraceutically acceptable or food grade excipient and wherein said formulation is further formulated in any oral formulation including tablet, capsule, chewing gum, candy lozenges, granules, powder or any liquid forms..

18. The herbal detox water formulation as claimed in claim 1 , wherein said water formulation further comprises one or more flavoring agents.

19. A method for preparing the herbal detox water formulation as claimed in claim 1 , wherein the method comprising:

extracting all or selected one or more plant parts of herbs by aqueous extraction, wherein the herbs used in concentrates are identified, isolated, extracted and characterized for the effective constituents present in said herbs;

filtering the one or more plant extract, concentrating the plant extract to achieve aqueous concentrate of selected plants at optimum temperature and pH;

blending the one or more plant aqueous concentrate to achieve a desired pH and stability of the herbal concentrate;

further, synergistically blending with natural antioxidants to achieve homogeneously clear concentrate, and

further developing the water formulation,

wherein the resultant water formulation is having desired pH value of 7.2-7.9.